DNA Necklac e Lab. Materials clear sports drink or 0.9% salt water Dixie cup Sharpie marker 15 mL...
-
Upload
brianna-mathews -
Category
Documents
-
view
214 -
download
1
Transcript of DNA Necklac e Lab. Materials clear sports drink or 0.9% salt water Dixie cup Sharpie marker 15 mL...
Materials• clear sports drink or 0.9% salt water• Dixie cup• Sharpie marker• 15 mL disposable test tube• test tube rack• microcentrifuge tube• transfer pipette• yarn (2 feet)• ice cold isopropyl, 70% ethanol,
or rubbing alcohol• diluted clear dish soap solution (50/50)
Slough off Cheek Cells
Swish the sports drink VIGOROUSLY for 60 seconds. You are trying to slough off as many cheek cells as possible.
Release the DNA from Cells
GENTLY mix the test tube by holding your thumb over the opening and inverting the test tube 5 times. DO NOT create bubbles.
5 times!
Release the DNA from Cells
The soap solution breaks the cell membranes and releases the DNA. Cell membranes are made of lipids (This is like soap breaking down grease on dishes.)
Separate out the DNA
Tilt the test tube.
GENTLY pour alcohol down the side of the test tube using your pipette until the total volume reads 13 mL.
You are trying to form 2 separate layers. DO NOT MIX!!!
Separate out the DNA
Let the test tube stand in the
rack for 10 minutes.
You will see white or clear
strands appearing – this is YOUR
DNA precipitating (solidifying)!
While waiting, get string and beads for your necklace.
(no more than 6 beads please)
Extract the DNAUse the pipette to GENTLY remove the strands of DNA and squirt it into your microcentrifuge tube.
Add a small amount of leftover liquid into the microcentrifuge tube to preserve your DNA.