DNA Methylation as a Biomarker for Early Life Adversity
-
Upload
beau-smith -
Category
Documents
-
view
24 -
download
0
Transcript of DNA Methylation as a Biomarker for Early Life Adversity
![Page 1: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/1.jpg)
![Page 2: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/2.jpg)
![Page 3: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/3.jpg)
![Page 4: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/4.jpg)
●○
●
●
![Page 5: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/5.jpg)
Limited access to brain tissues in humans difficulty identifying early-life adversity biomarkers
Past Research findings:
1. Early-life exposures are linked to epigenetic disruptions2. BPA has been shown to disrupt neurodevelopment in both animals and humans3. BDNF gene encoding brain-derived neurotrophic factor is essential in in
neurodevelopment
The main idea:Develop series of assays to test whether the methylation of the BNDF gene could serve as biomarker for early life adversity
![Page 6: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/6.jpg)
![Page 7: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/7.jpg)
Mice maintained in a caged environment- access to food, water, and normal light cycles
Variable group: treated with BPA in dissolved in corn oil Control group: treated with only corn oil
Allowed to mate → mice that became pregnant were placed in a new cage for the gestation period
![Page 8: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/8.jpg)
Day 1-6: Mother-infant interactions monitored and maternal behavior was noted: Licking, grooming, arched-back nursing
Day 28: 6 male and 6 female offspring were killed, whole brains extracted gene expression of neurotrophic factorsDNA methylation in hippocampus
Day 60: Object Recognition Test to Assess Behavior (Assessing Hippocampus)assess the time it takes exploring object and its preference for novel object
whole brains and whole-blood samples:Assess correlation between methylation in blood→ hippocampus
![Page 9: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/9.jpg)
![Page 10: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/10.jpg)
![Page 11: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/11.jpg)
●●
●
●
![Page 12: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/12.jpg)
●●
●
●
![Page 13: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/13.jpg)
●●
●
●
●
![Page 14: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/14.jpg)
●
●
●
●
![Page 15: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/15.jpg)
●
●●
●●
●●●
![Page 16: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/16.jpg)
![Page 17: DNA Methylation as a Biomarker for Early Life Adversity](https://reader031.fdocuments.in/reader031/viewer/2022030307/58ea200b1a28abf9018b4e1d/html5/thumbnails/17.jpg)
●
●
●
●
●