Department of Biopharmaceutics and PharmacodynamicsFaculty of Pharmacy, Medical University of Gdańsk

Head: Prof. Roman Kaliszan, Ph.D., D.Sc.Corresponding Member of the Polish Academy of Sciences

Prof. Antoni Nasal, Ph.D., Barbara Damasiewicz, Ph.D., Michał Markuszewski, Ph.D., Aleksandra Radwańska, Ph.D., Danuta Siluk, Ph.D., Tomasz Bączek, Ph.D., Teresa Frąckowiak, Ph.D., Anna Wojdełko, M.Sc., Michał Marszałł, M.Sc.,

Paweł Wiczling, M.Sc., Aleksandra Dąbrowska, Ing., Janina Kozłów

Address: Gen. J. Hallera 107, 80-416 Gdańsk

Tel.: ++48 58 3493260

Fax: ++48 58 3493262

e-mail: roman.kaliszan@amg.gda.pl

Wydział Chemii Uniwersytetu Gdańskiego

Instytut Farmaceutyczny w Warszawie

Instytut Leków w Warszawie

Zakład Chemii Środowiska Wydziału Chemii Uniwersytetu Mikołaja Kopernika w Toruniu

Instytut Rozrodu Zwierząt i Badań Żywności PAN w Olsztynie

Department of Chemistry, University of Edinburgh

Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe University, Frankfurt am Main

Forschungsinstitut für experimentelle Biologie und Medizin, Borstel

Department of Chemistry, Rutgers University, New Brunswick

Department of Oncology, McGill University, Montreal

Department of Chemistry, State University of New York in Binghamton

Institut für Organische Chemie, Abteilung Bioorganische Chemie, Universität Bremen

Department of Chemistry, University College London, London

Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest

Laboratory of Instrumental Analysis, University of Technology, Eindhoven

LC Resources Inc., Walnut Creek, CA, USA

School of Materials Science, Toyohashi University of Technology,

Department of Polymer Science and Engineering, Kyoto Institute of Technology

Faculty of Science, Himeji Institute of Technology

COLLABORATORS

PUBLICATION RECORDS

Books 10Review Articles 14Original Reports 160Full Papers in Symposium Proceedings 30Patents 6Symposium Abstracts 210Published book reviews, popularization of science, etc. 50

Σ 480

Σ IF>250Total number of citations 3.000

MEMBERSHIP OFEDITORIAL (ADVISORY) BOARDS

- Solubility Data Series (1985 - 1992)- Quantitative Structure-Activity Relationships (1988 - 1997)

-Biomedical Chromatography (since 1998)- Journal of Chromatographic Science (since 2000)

- Journal of Separation Science (since 2001) - Journal of Pharmaceutical and Biomedical Analysis (since 2001)

 - Acta Chromatographica (since 1995)-Acta Poloniae Pharmaceutica - Drug Research (since 1997)

- Annales Universitatis Mariae Curie-Skłodowska -Annales Academiae Medicae Gedanensis

-Farmacja Polska- Gazeta AMG

- Służba Zdrowia- Wiadomości Farmaceutyczne

About 400 reviews of manuscripts presented to some 30 international journals

CHOICE OF STRUCTURE

H

H

H

H

H

H

H

COOH

Example: S = 20; N = 8

X = SN = X = 208 = 25 600 000 000

N - number of substitution sites S - number of substituents

CA describes 12 000 000 compounds

Numerical descriptors of solute structures

Chromatographic retention parameters

Computerized statistical data processing

QSRR

Identification of informative descriptors

Determination of molecular separation

mechanism

Quantitative comparison of

columns

Retention prediction

Determination of complex physicochemical properties

Evaluation of relative biological activity

OOOOOOOOOOOOOOO

OOOOOOOOOOOOOOO

 

WATERI 

pH1 

Drug molecules I: 

neutral I + ionized I

 

WATERII 

pH1I 

Drug molecules II: 

neutral II + ionized II

Example:

Kanamycin: pKa = 7.2

Milk: pHI = 6.4

Blood: pHII = 7.4

Lipid

Drug:Water/lipid partition (log P, log kw);

Degree of ionization (pKa)

 

[Drug in milk]

[Drug in blood]

[1 +10 ]

[1 +10 ]

pK pH

pK pH

a milk

a blood

4 5.       

IMMOBILIZED ARTIFICIAL MEMBRANES

Structure of cell membrane

Hydrophobic region

Hydrophilic region

IAM.PC.(ether)IAM.PC.DD.IAM.PC.(ester)

SILICA SUPPORT

HNC O

NC O

HNC O

NC O

HNC O

HNC O

SILICA SUPPORTSILICA SUPPORT

H2NH2NOC

N

CO

OO

CO

OP O-

OO

N+H3C

H3CH3C

OP O-

OO

N+H3C

H3CH3C

OO

P O-O

O

N+H3C

H3CH3C

OCH3

CHROMATOGRAPHIC MODELING OF DRUG-PROTEIN INTERACTIONS (HSA, AGP, Melanin, Keratin, Collagen)

STERIC RESTRICTION

HYDROPHOBIC REGIONHYDROPHOBIC REGION

+

⊝⊝⊝ANIONIC REGION

FUTURE PROJECTS AND GOALS

- pH-Gradient HPLC: a new method of chromatographic analysis of

bioanalytes

- Metabolomics: metabolite profile differentiation using LC/CE/MS

and bioinformatics

-Analytical separation-identification platform LC-MC for the analysis

of proteomes

- Relationship between the GPIIIa gene A1/A2 polymorphism and

blood platelet response to aggregation agonists among the North

Poland population

SELECTED RECENT PUBLICATIONS•R. Kaliszan: "Quantitative Structure-Retention Relationships (QSRR) in Chromatography", In Encyclopedia of Separation Science, Vol III, I.D. Wilson (Ed.), Academic Press, San Diego, 2000, pp. 4063-4075 •D. Siluk, R. Kaliszan, P. Haber, J. Petrusewicz, Z. Brzozowski, G. Sut, Antiaggregatory activity of hypoglycaemic sulphonylureas, Diabetologia 45, 1034-1037 (2002) •R. Kaliszan, P. Haber, T. Bączek, D. Siluk, K. Valko, Lipophilicity and pKa estimates from

gradient high-performance liquid chromatography, J. Chromatogr. A 965, 117-127 (2002) •M. Markuszewski, R. Kaliszan, Quantitative structure-retention relationships in affinity high-performance liquid chromatography, J. Chromatogr. B 768, 55-66 (2002) •A. Nasal, D. Siluk, R. Kaliszan, Chromatographic retention parameters in medicinal chemistry and molecular pharmacology, Curr. Med. Chem. 10, 381-426 (2003)

•M.J. Markuszewski, P. Britz-McKibbin, S. Terabe, K. Matsuda, T. Nishioka, Determination of pyridine and adenine nucleotide metabolites in Bacillus subtilis cell extract by sweeping borate complexation capillary electrophoresis, J. Chromatogr. A 989, 293-301 (2003)

•T. Bączek, R. Kaliszan, Predictive approaches to gradient retention based on analyte structural descriptors from calculation chemistry, J. Chromatogr. A 987, 29-37 (2003)