Dehydrated Culture Media - ESI.infocms.esi.info/Media/documents/ColeP_microbiology_ML.pdf ·...

Subhead, sub-subhead1/sub-subhead2MI Microbiology

902 l U.S. 800-323-4340 toll-free, 847-549-7600 (outside the U.S.), www.coleparmer.com Canada 800-363-5900 l India 91-22-6716-2222 l UK 0500-345-300

902

Table-of-ContentsWebRegistration

Catalog number Media Description Size (g) PriceKH-14200-10 Agar, bacteriological This clear gel agar is high grade and manufactured using the ice method 500 KH-14202-00 Azide dextrose broth Use for detection of gecal Streptococci in water and sewage 500 KH-14202-01 Baird parker agar Use for the selective isolation of coagulase positive Staphylococci in food 500

KH-14202-02 Blood agar base with low pH Use to cultivate a wide variety of bacteria. It is usually prepared with blood to observe hemolytic reactions 500

KH-14202-03 Bile Esculin agar This is used as a differential medium in the isolation and presumptive identification of enterococci/group D streptococci 500

KH-14200-12 Brain heart infusion agar Use to cultivate a wide spectrum of bacteria, yeasts, and molds 500 KH-14202-05 Brain heart infusion broth Use to cultivate fastidious aerobic and anaerobic bacteria 500 KH-14200-14 Brilliant green bile broth A standard methods medium for confirmed and completed tests for coliforms in water 500 KH-14202-08 Buffered peptone water Helps in the recovery of Salmonella from foods after preservation techniques 500 KH-14200-52 Cetrimide agar Use for the selective isolation of Pseudomonas aeruginosa 500

KH-14202-11 D/E neutralizing Broth This broth is especially suited for environmental sampling where neutralization of the chemical is important to determining the bactericidal activity. 500

KH-14202-12 EC Use to isolate E. coli from water, milk, and shellfish; and for differentiation of coliform bacteria at 37°C and E. coli at 45.5°C 500

KH-14202-14 Eosin methylene blue agar, Levine Use this selective medium to detect gram negative enteric bacteria. Contains double the concentration of lactose found in eosin methylene blue (HH&T), and no sucrose. 500

KH-14202-16 Fluid thioglycollate medium Use to test sterile materials for the presence of aerobic, anaerobic, and microaerophilic microorganisms 500 KH-14202-18 Fraser broth base Use to isolate Listeria sp. from food and environmental materials 500 KH-14202-20 GC agar An enriched medium for the growth of fastidious bacteria when used with supplements and hemoglobin 500 KH-14202-22 Hektoen enteric agar Use this selective, differential medium to isolate Salmonella and Shigella from clinical materials 500 KH-14202-24 Lactobacilli MRS broth Use to cultivate Lactobacilli from dairy products and other food materials 500 KH-14202-26 Lactose broth A standard methods medium for the growth of coliform bacteria from water, food, and dairy products 500 KH-14202-30

Lauryl tryptose broth A standard methods medium for the growth of coliform bacteria in water and food500

KH-14202-32 2 kg KH-14202-34 Letheen broth base, AOAC Use to test items sanitized with quaternary ammonium compounds 500 KH-14202-36 Listeria enrichment broth A selective enrichment of Listeria monocytogenes from milk 500 KH-14202-37 m-endo agar Recommended media for the enumeration of coliforms in water by membrane filtration. 500 KH-14202-38 m-Enterococcus agar Use this highly selective medium to isolate Enterococci from water, food, and other materials 500 KH-14200-44 m-FC agar Use to detect fecal coliforms at elevated temperatures by membrane filter technique 500 KH-14200-22 MacConkey agar Use as a selective and differential medium for the detection of coliforms and other enteric organisms 500

KH-14202-44 Mannitol salt agar Use this selective medium to isolate and enumerate Staphylococci. Recommended by the USP for microbial limit tests 500

KH-14202-46 Mueller Hinton agar Use for the determination of antibiotic susceptibility in the disk-agar diffusion method 500 KH-14200-24 Nutrient agar A general purpose medium for the cultivation of nonfastidious organisms 500 KH-14202-50 Nutrient gelatin Use to detect gelatin liquefaction 500 KH-14200-26 Pancreatic digest of casein Use this general-purpose peptone to promote growth in various media formulations 500 KH-14202-54 Pancreatic digest gelatin Use this general-purpose peptone to promote growth in various media formulations 500 KH-14200-54 Phosphate buffer; pH 7.2 Use to prepare dilution blanks for samples of water, food, and dairy products 500 KH-14200-28 Potato dextrose agar Use this standard methods medium to culture yeasts and molds from dairy and other food products 500 KH-14202-60 Rappaport-Vassiliadis Use in the AOAC method to rapidly detect motile Salmonella in food products 500 KH-14202-62 Sabouraud dextrose agar Use this USP medium to perform total combined yeast and mold counts (microbial limit tests) 500

KH-14202-64 Selenite cystine broth Use this USP and AOAC enrichment medium to detect Salmonella in food and dairy products, and other materials 500

KH-14202-66 Skim milk Use as a media ingredient 500 KH-14200-32 Standard methods agar Use this standard methods medium to analyze water, food, and dairy products 500

KH-14200-48 Tetrathionate broth base Use this standard methods medium for the enrichment of Salmonella from samples of water, food, and clinical material 500

KH-14202-72 Triple sugar iron agar Use to identify gram negative enteric organisms based on carbohydrate fermentation and H2S production 500

KH-14200-34Tryptic soy agar Use this USP medium for the total aerobic portion of microbial limit testing.

Also use as a primary media for preparing blood plates500

KH-14202-75 2 kg KH-14200-36

Tryptic soy broth A USP soybean-casein digest broth for sterility testing and general-purpose use500

KH-14202-77 2 kg KH-14202-80 UVM listeria broth A selective enrichment medium for Listeria monocytogenes 500

KH-14202-82 Violet red bile agar Use this standard methods medium for the selective detection of coliforms in water, dairy products, and other food materials. 500

KH-14202-84 XLT4 agar A selective medium with Tergitol 4 added for the detection and isolation of nontyphi Salmonella 500 KH-14202-86 Yeast extract This is the water soluble portion of autolysed yeast; a water soluble, clear solution after autoclaving 500

Dehydrated Culture MediaEconomically priced media cultures are tested and standardized to ensure consistent and reliable performance

ColeParmer.com/Microbiologyfor our most up to date product selection.

Each order includes a certificate of compliance and quality control that shows lot numbers, expiration dates, and a shelf-life guarantee. Wide mouth openings on bottles let you scoop out powder efficiently with-out dusting the countertop. The lightweight plastic bottles have indented hand grips for steady handling. Wide, flat cap allows you to stack containers and save space. Media are formulated to dissolve quickly.

Subhead, sub-subhead1/sub-subhead2MIMicrobiology


903


Media Broth AmpulesThese presterilized plastic ampules contain 2 mL of broth for convenient direct-plating of samples using our standard monitors (sold above). We recommend keeping a 6-to 8-week supply on hand—refrigerate until use. Box of 50.

Microbiological monitors

Media broth ampules

Catalog number Description Price/bxStandard monitors

KH-29823-10 Monitor with white grid filter membrane KH-29823-14 Monitor with black grid filter membrane KH-29823-12 Monitor with white grid filter membrane, pre-plugged and wrapped

Monitors with removable filtersKH-29823-15 Monitor with white grid filter membrane, pre-plugged KH-29823-16 Monitor with black grid filter membrane, pre-plugged

*The m-Endo broth comes in a plastic-covered glass ampule for stability.

Catalog number Broth Applications Shelf life Price/bx

KH-14020-00 m-Endo* Selective enumeration of E. coli and coliform bacteria in water and food 12 mo

KH-14020-01 m-FC Detection and enumeration of fecal coliform organisms 12 mo

KH-14020-02 m-Green yeast and mold

Nonselective yeast and mold detection for soft drinks and other fluids 12 mo

KH-14020-03 m-TGE total count

Nonselective, general determination of bacterial counts in water, milk, and other fluids 12 mo

KH-14020-04 MacConkey with MUG

Selective determination of E. coli populations in water, wastewater, and food 6 mo

KH-14020-05 m-Staphylococcus Isolation of pathogenic staphylococci (High selectivity restricts growth of most other bacteria.) 12 mo

KH-14020-06 TCBS (Vibrio spp)

Selective growth of Vibrio cholerae and Vibrio parahaemolyticus 12 mo

KH-14020-08 m-Enterococcus Selective isolation/enumeration of enterococci in water, food, and fluids. Highly selective for fecal streptococci. 9 mo

KH-14020-09 Cetrimide Selective isolation/identification of Pseudomonas aeruginosa 9 mo

KH-14020-10 Tergitol 7 with TTC

Selective identification/enumeration of coliform bacteria 18 mo

KH-14020-11 VRBG Selective enumeration of coliform bacteria in food/dairy products 6 mo

KH-14020-12 Selective yeast and mold

Selective determination of yeast and mold counts in fluids. (Chloramphenicol inhibits most bacteria.) 12 mo

KH-14020-13 Tryptic soy General purpose cultivation of fastidious and non-fastidious microorganisms 12 mo

Microbiological Monitors and Media Broth AmpulesAll-in-one filtration and incubation device for convenient direct plating

Microbiological MonitorsEach monitor features a 100-mL graduated funnel for filtering up to 100 mL of sample. Once sample is filtered, monitor converts to a petri dish for incubation—simply add media broth (sold separately below). Monitors include a 47-mm diameter membrane filter with 0.45 µm pore size. Monitors require media broth ampules below for testing. All monitors are single-use, presterilized filtering units with welded fixed-membranes and culturing devices. Order monitors with removable filters to culture bacteria on agar plates. Box of 50.

Note: Monitors require a no. 8 stopper and a manifold for filtration application; order vacuum filtration manifold separately from our “Filtration—Laboratory” section on pages 445–470.

Analytical Funnels Analytical funnels are ready-to-use 100-mL filtration units with removable membrane and culturing devices. After filtration, the membrane of the analytical funnel can be used for a wide range of qualitative and quantitative biological analysis. Once sample is filtered, remove the upper part from the base and put the base on the membrane lifting device 14010-16. Separate the membrane from the pad and transfer the membrane into a petri dish with a sterile pad.

Catalog number Description Qty/box Price/bxKH-14010-10 Analytical funnel, 0.2 µm, white/black grid 50 KH-14010-12 Analytical funnel, 0.45 µm, white/black grid 50 KH-14010-14 Analytical funnel, 0.45 µm, black/white grid 50

KH-14010-16 Membrane removal/lifting device KH-02902-50 Filter forceps; flat tip, 41⁄2"L, 304 stainless steel

Work Flow I—Microbiological Monitors1. Sample filtration2. Remove funnel3. Add 2 mL of microbiological media4. Replace the lid and incubate

Work Flow—Analytical Funnels1. Sample filtration2. Remove the upper funnel body from the base3. Place the base on the membrane lifting device4. Separate the membrane from the pad and

transfer the membrane into a petri dish with a sterile pad

Analytical funnelUse removal/lifting device

14010-16 to transfer membrane after filtration.

Upper funnel body

Lifting device

Filter forcep



904


BioSart® 100 Monitors and 250 Funnels Designed for colony and particle counting BioSart products are specially designed for microbiological testing and analytical quality assurance. Typical applications include fast filtration required in the routine testing of water, food and beverages, pharmaceutical, and cosmetic products.

BioSart 100 is an easy-to-handle, 100-mL disposable monitor with polystyrene housing, polyethylene adapter, a 47-mm cellulose nitrate membrane, and cellulose pad. It is a ready-to-use filter unit designed to be placed onto the base of a vacuum manifold. Available in three filter colors for ease of identification. Gamma irradiated; individually sterile packaged.

BioSart 250 funnel is made of polypropylene and is sufficiently elastic for optimal sealing with a bayonet-type closure. It fits 47-mm diameter filter membranes (not included). Graduations are marked at 50, 100, 150, and 200 mL for exact sample volumes. The large inner diameter ensures a high flow rate. No liquid is retained in the filter funnel after filtration. ETO presterilized.

Catalog number Description Capacity Filter

diameterPore size

Filter area Qty/pk Price/pk

BioSart 100 monitors with filter membraneKH-81055-00 Monitor with white/black grid 100 mL 47 cm 0.2 µm 14.5 cm2 48 KH-81055-02 Monitor with white/black grid 100 mL 47 cm 0.45µm 14.5 cm2 48 KH-81055-04 Monitor with green/dark green grid 100 mL 47 cm 0.45µm 14.5 cm2 48 KH-81055-06 Monitor with gray/white grid 100 mL 47 cm 0.45µm 14.5 cm2 48

BioSart 250 funnelsKH-81055-08 Sterile packaged 250 mL 47 cm — 12.5 cm2 50 KH-81055-10 Individually sterile packaged 250 mL 47 cm — 12.5 cm2 50

Specifications & Ordering Information

81055-00

81055-08

Nutrient Pad Sets Dehydrated media pads in petri dishes, with matching membrane filters l Economical, time-saving microbiological quality controll Room temperate storage with 9 to 24 months of shelf lifeEach set consists of a 50-mm pad, evenly impregnated with nutrient media, packed dry and sterile in a petri dish, plus a suitable 47-mm diameter membrane filter, sterile and individually packed. They are ready to use immediately after they have been reactivated by wetting them with 3.5 mL of sterile, demineralized, or distilled water. Available in a variety of types of culture media, nutrient pad sets come with the appropriate membrane filters to ensure optimal retention and colony growth. Each pack contains 100 sterile nutrient pad sets.

Major application includes microbiological testing of relatively large sample volumes that have a low number of microbes using the membrane filtration method. a high flow rate. No liquid is retained in the filter funnel after filtration. ETO presterilized.

Catalog number Determination detection Media typeIncluded membrane filter

Price/pk of 100Grid Pore size

KH-81055-20 Pseudemonas aeruginosa Cetrimide White/green 0.45 µm KH-81055-22

E. coli and coliforms

Endo

White/green 0.45 µm

KH-81055-24 M-FC KH-81055-32 Teepol KH-81055-34 Tergitol TTC KH-81055-26

Colony count

R2A

Green/dark green 0.45 µm

KH-81055-28 Standard KH-81055-30 Standard TTC KH-81055-36 TGE

KH-81055-38 Lactic acid bacteria and other beverage spoilers Wallerstein White/black 0.45 µm

KH-81055-40 Yeast and moulds Wort Gray (black after wetting)/white 0.65 µm


81055-20

Autoclavable Dosing SyringeThe dosing syringe is perfectly suitable in connection with a filter holder or a syringe filter for rapid, simple filtration. Ideal for moistening the nutrient pad sets with sterile water. Syringe features a three-way valve that allows the gun to pull in buffers or water and dispense at the same time. Volume can be infinitely adjusted from 0.5 to 5.0 mL by turning the screw at the handle.

Catalog number Description Volume PriceKH-81055-90 Dosing syringe 0.5 to 5.0 mL

Specifications & Ordering Information81055-90

Colony count is the quantitative determination of microorganisms present in a sample. The quantity can represent either the total bioburden or the detection and quantification of specific microorganisms. The counts are expressed in colony-forming units per 1 mL sample volume (CFU/mL).

Syringe filters are not included. Order separately

from filtration section pages 445–471.



905


37-mm Monitors for Microbial/Contamination AnalysisIdeal for bacterial, yeast, mold and other contamination analysis Pre-assembled two- and three-piece units contain a built-in, 37-mm membrane filter and support pad. Monitors are suitable for field collection and transportation of samples as well as analysis in the laboratory. Samples collected on the press-fit membrane can be cultured in place or removed for further analysis. Monitors come presterilized and individually packed. Monitor sets include a two-piece monitor, plug, sampling tube, and three-way connector.

Catalognumber Analysis type

MembraneSterile Qty/

pkPrice/

pkSurface Pore size ColorThree-piece monitors

KH-02935-00 Aerosol, contamination Plain 0.45 µm White No 50 KH-02935-02 Bacterial, contamination Gridded 0.45 µm White Yes 50 KH-02935-04 Black Yes 50 KH-02935-06

Yeast/mold

Plain 0.80 µm White No 50 KH-02935-08

Gridded 0.80 µm White Yes 50 KH-02935-10 No 50 KH-02935-12 Black No 50 Two-piece monitorsKH-02935-14

Bacterial, contamination Gridded 0.45 µmWhite Yes* 50

KH-02935-16 Yes 50 KH-02935-18 Black Yes* 50 KH-02935-20 Yes 50 Two-piece monitor setsKH-02935-22 Bacterial, contamination Gridded 0.45 µm White Yes* 20 KH-02935-24 Black Yes* 20

*With hydrophobic edge.

Materials Housing: styrene acrylonitrile Membrane: mixed cellulose ester (nitrocellulose) Support pad: pure cotton cellulose

Filtration area: 9.0 cm2 (6.7 cm2 for hydrophobic edge type)

Maximum temperature: 45°C


02935-00

Water and Surface Microbial Testing Incubator/AutoanalyzerComplete system automatically provides quick, accurate results l Run two different test simultaneously with results provided

in a matter of hoursl Displays in direct reading CFUs

Incubator/autoanalyzer features self-test, automatic incubation control, end of test detection, and an easy-to-clean membrane keypad. Results display in 0.1 order of magnitude increments over the testing range of 10 to 108 cfu. Additional features include an integrated RS-232 port, multi-level incubations, bacteria growth curves plotting, and calibration curves for IME Test ampoules. In the event of power loss, system memory retains test status so that it can be continued at another time. Use with IME Test™ kits, sold separately at right.

Test parameter Range (cfu/mL*) Catalog number Price/pk of 30Total viable microbes, chemically treated samples 10 to 108 EK-99554-02

Coliform (total and fecal) 2 to 2.5 x 106 EK-99554-04

E. Coli (total and fecal) 0 to 18 or 1.0 x 102 to 9.9 x 106 EK-99554-06

Sulfur microbes 0 to 1,000,000 EK-99554-08 Sulfur microbes, chemically treated samples 0 to 1,000,000 EK-99554-10

Test KitsWhile submerged in liquid sample, snap the tip of the ampoule against the inside edge of the test kit sample cup. The proper sample amount is automatically drawn—reducing the chance of accidental cuts and contamination. Order dry surface sampler (99554-12) and food and objects sampler (99554-14) separately to test solid samples.

What’s included: 30 test ampoules, one sample cup, and one color standard/results chart. Kits 99554-02 through -06 also include dechlorinating solution.

*cfu/mL = colony forming units per milliliter

EK-99553-50 Battery pack. Includes 9 V battery, charger, and 12 VDC adapter EK-99553-60 Carrying bag, small; 11"W x 91⁄4"H x 41⁄2"D EK-99553-65 Carrying bag, large; 15"W x 91⁄4"H x 8"D EK-99554-12 Dry surface sampler, sterile. Includes sponge and rinse solution EK-99554-14 Food and objects sampler, sterile. Includes sponge and rinse solution

Number of cells: twoDisplay: LCD, 2 x 16 charactersResolution: 0.1 cfu/mLSource lamp: LEDDetector: silicon diodeOutput: RS-232

Operating ambient: 35 to 95°F (2 to 35°C)

Power: 110 VAC, 9 V battery, or 12 VDC (battery pack and 12 VDC adapter sold separately)

Dimensions: 41⁄8"L x 51⁄8"W x 61⁄2"H


Catalog number Description PriceKH-99533-40 Incubator/autoanalyzer with 110 VAC adapter

99533-40

For a complete selection of colony counters.

913–914

For a complete selection of water sampling equipment.

2002–2014

For a complete selection of water testing equipment.

2015–2034



906


Gridded Membrane FiltersThese gridded membrane filters are made of cellulose nitrate—a material which ensures effective retention with high flow rates and optimal colony growth.

Sterile Filters are individually packed to avoid the possibility of contamination. These filters conform to EPA and GLP, having filter identification and lot number printed on each individual envelope. The included certificate documents quality assurance tests and compliance of the 0.45 µm membrane filter with ISO 7704.

Nonsterile Filters are not individually packed. They are used for particle testing, microscopy, and microbiology.

Membrane: cellulose nitrateFilter diameter: 47 mm

Membrane thickness: 90 to 140 µm

Catalog number Application Grid Pore size Qty/pk Price/pkSterile gridded membrane filters

KH-81055-50

Bacteriological research White/black0.80 µm 100

KH-81055-52 0.45 µm 100 KH-81055-54 0.20 µm 100 KH-81055-60 Green/dark green 0.45 µm 100 KH-81055-56 Yeast and mold detection Gray*/white 0.80 µm 100 KH-81055-58 0.45 µm 100

KH-81055-62 E. coli, coliform, and other bacteria detection White/green 0.45 µm 100

KH-81055-64Use with auto dispenser

White/black 0.45 µm 300 KH-81055-66 Gray*/white 0.45 µm 300 KH-81055-68 Green/dark green 0.45 µm 300

Nonsterile gridded membrane filtersKH-81055-70

General purposeWhite/black

1.2 µm 100 KH-81055-72 0.80 µm 100 KH-81055-74 0.45 µm 100 KH-81055-76 Gray*/white 0.80 µm 100 KH-81055-78 0.45 µm 100 KH-81055-80 Quality assurance of antibiotics White/black; with 3-mm

pink, hydrophobic edge0.45 µm 100

KH-81055-82 0.20 µm 100 *Black after wetting


Mixed cellulose ester membranes are intended for use in the biological analysis of water and beverages. Presterilized, 47-mm dia. Order membrane with absorbent pads for use with broth media. Gridded.

Sterile Mixed Cellulose Ester Filter Membranes

Catalog number Pore size Absorbent pads Packaging Qty/pk Price/pkMixed cellulose ester membranes

EK-06644-08 0.45 µm0.45 µm0.65 µm

IncludedNot included

Included

10 membranesper envelope

100200100

EK-06644-10EK-06644-06EK-06644-14 0.45 µm

0.45 µm0.65 µm

Not includedIncluded

Not included

1 membraneper envelope

100100100

EK-06644-16EK-06644-12

Whatman mixed cellulose ester membranesEK-29877-01 0.45 µm Included 1 membrane

per envelope1001000 EK-29811-52


06644-08

06644-14

Colony count is the quantitative determination of microorganisms present in a sample. The quantity can represent either the total bioburden or the detection and quantification of specific microorganisms. The counts are expressed in colony-forming units per 1 mL sample volume (CFU/mL).A membrane filter of the appropriate pore size is placed in a filter holder, and the sample is then filtered. In this process microorganisms in the test sample are retained on the filter surface by the screening action of the membrane filter. For a simple evaluation of the results, the grid divides the filtration area into 130 squares, each measuring 3.3 x 3.1 mm.

81055-52

81055-62

81055-60

81055-58



907


Disposable Absorbent Pads with Dispenser, Sterile

Easy to dispense, ETO presterilized These 1.4-mm thick absorbent pads are made of cellulose. Wetted with liquid culture medium before a membrane filter is placed on them. They come presterilized in 10 plastic tubes. Each tube includes 100 pads and two manual dispensing devices.

Specifications & Ordering InformationCatalog number Description Dia Qty/tube Qty/bx Price/bx

KH-81055-42 Disposable absorbent pads with dispenser 47 mm 100 1000

81055-42


MI Agar and MI BrothFor the simultaneous detection of total coliforms and E. coli in waterl EPA approved for quantitative and traditional presence/absence testing (method 1604)l Rapid process, no confirmation stepl Positive results in as few as 16 hours

MI agar is the only membrane filtration method that is US EPA approved for use under both the Total Coliform Rule for drinking water and the Surface Water Treatment Rule for source water. MI agar and MI broth offer a quicker, easier, and more accurate solution to simultaneous determination and enumeration of coliforms and E. coli. Get your results in hours, not days; results are easy to interpret and with 95% accuracy and no confirmation step. Positive identification of total coliforms is indicated by the development of fluorescence under UV light; E. coli is indicated by the development of fluorescent blue colonies.

14030-3014030-28

Catalog number Description Size Qty/pk Price/pkKH-14030-26 Ampouled media 2 mL 50 KH-14030-28 Bottled agar 50 mL 1 KH-14030-30 Bottled broth 50 mL 1 KH-14030-32 Dehydrated broth 500 g 1

14030-26

A

A Gram Stain Set (with stabilized iodine)

Designed for staining microorganisms from cultures or specimens by the differential Gram method. Packaged in squeezable bottles with screw-on dispenser caps. Set includes one 250-mL bottle each of stabilized iodine, crystal violet, safranin, and decolorizer.

B Gram Stain Replacements

Description250-mL bottles 1-gal. bottles

Cat. no. Price/pk of 4 Cat. no. Price/eaGram iodine, stabilized — — KH-14204-38 Gram iodine, traditional KH-14204-30 KH-14204-40

Gram crystal violet KH-14204-24 KH-14204-34 Gram safranin KH-14204-32 KH-14204-42

Gram decolorizer — — KH-14204-36

ACCRA LAB® Gram StainsProduce brilliantly stained specimensl Ultrastable and ready to useACCRA LAB® Gram stain reagents offer purity, stability, and superior quality. These Gram stains eliminate interference from background staining, producing brilliantly stained specimens. Manufactured according to traditional formulations that are used by most laboratories. Decolorizer is an acetone/isopropanol (1:4) mixture. All stains are packaged in polyethylene containers. Store at controlled room temperature; 24-month shelf life.

14204-20

Catalog number Description Price/stKH-14204-20 Stabilized Gram stain set

B

Disposable 50-mm Petri Dishes with Absorbant PadsEK-14005-20 Disposable 50-mm Petri Dishes with absorbant pads; 100/cs



908


Sterile Contact Plates, PS

For determining surface microbial contamination. Dishes have a raised agar bed for surface contact. The grid consists of sixteen 10 x 10 mm squares marked numerically in one direction and alphabetically in the other. Disposable polystyrene. Radiation sterilized. Supplied 20 per polyethylene sleeve.

Brand 3160 Petri DishesFlat, clear dishes will withstand repeated sterilization (wet or dry). The covered dish is not airtight. The tops are marked in blue enamel and the bottoms in white enamel to make sorting easier. Bottoms also have a triangular enamel reference point for serial dilutions.

34551-03

Chemware® Petri Dishes, PFA

Ideal for contamination-free biological applications. Feature an inert, nonstick, noncontaminating surface. Lids not included. Autoclavable.

Catalog number Capacity Dia x H (mm) Qty/pk Price/pkEK-06148-00 60 mL 50 x 15 1 EK-06148-02 100 mL 100 x 15 1

Autoclavable Petri Dishes, PMP

Transparent, shatterproof dishes are nontoxic. Raised stacking ridges on the base prevent dishes from sticking to wet surfaces. Polymethylpentene dish has superior chemical resistance and resists cell adhesion. Autoclavable.

06143-20

06148-00

Catalog number Dia x H (mm) Qty/pk Price/pkKH-06143-20 100 x 15 10

Sterile Petri Dishes, PS

Disposable polystyrene (PS) dishes have convenient stacking ridges. Sterilized by gamma irradiation; packaged in easy-open bags of 20. 06139-00 06139-01

06139-02

Catalog number Dia x H (mm) Qty/pk Price/pkKH-06139-01 60 x 15 500 KH-06139-00 100 x 15 500 KH-06139-02 150 x 20 100

Catalog number Dia x H (mm) Qty/cs Price/csKH-14006-50 65 x 15 500

14006-50

Handbook of Microbiological MediaA compilation of formulations, methods of preparation, and uses for several thousand different media. Includes media for several specific methods, plus lists of classic and modern media used for identification, cultivation, and maintenance of diverse bacteria.

EK-15316-08 Handbook of Microbiological Media, 2nd Edition R. Atlas, 1997. 1079 pp. ISBN 0-8493-2638-9.

Dia x H(mm)

Catalog number

Qty/pk

Price/pk

Catalognumber

Qty/cs

Price/cs

60 x 15 EK-34551-01 12 EK-34551-21 72 100 x 10 EK-34551-02 12 EK-34551-22 72 100 x 15 EK-34551-03 12 EK-34551-23 72 100 x 20 EK-34551-04 12 EK-34551-24 72 150 x 15 EK-34551-05 12 EK-34551-25 24 150 x 20 EK-34551-06 12 EK-34551-26 24



909


Petri Dish RacksSpace-saving, acrylic racks permit easy viewing of incubating petri dishes. Polycarbonate posts. For use with plastic dishes only.

Catalog number Capacity Dimensions (L x W x H) Price/eaKH-06146-00 56 dishes, 60 mm dia 101⁄2" x 61⁄2" x 51⁄2" KH-06146-10 24 dishes, 100 mm dia 81⁄8" x 4" x 8" KH-06146-30 60 dishes, 100 mm dia 13" x 77⁄8" x 73⁄4"

Lab-Tek® Sterile Petri Dishes, PS

These sterile and disposable polystyrene (PS) dishes are ideal for culturing fungi, bacteria, and other microorganisms. Dishes work well in robotic dispensers due to flatness and uniform height. Model 14007-10 is square in shape. Model 14007-04 is 10 mm deeper than standard dish depth to accommodate longer culture periods. Sterilized by gamma irradiation.

Catalog number Description Dia x H (mm) Qty/cs Price/csKH-14007-03

Standard 60 x 20 400

KH-14007-01 100 x 15 320 KH-14007-05 140 x 20 180 KH-14007-04 Deep 100 x 25 300 KH-14007-10 Square 100 x 15 500

Disposable 50-mm Petri Dishes with Absorbent PadsIdeal for culturing microorganisms in water/wastewater testingStandard 50 x 11 mm polystyrene dishes preloaded with 47 mm absorbent cellulose pad (0.85±0.17 mm thick). Pads are tested for neutrality and for absorption volume of 1.8 to 2.0 mL of Endo medium. Individual presterilized pads can also be purchased separately below. Manufacturer’s certification of compliance available upon request.

Catalog number Description Dia x H (mm) Qty/cs Price/csKH-14005-20 Petri dish with pads 50 x 11 100

KH-14005-22 Presterilized absorbent pads, box of 100 individual package

06146-30

Catalog number Fits flask size Qty/pk Price/pkEK-06299-00 125 to 2000 mL 10

Sterile Venting Closure, Silicone

Ribbed, vented closure offers greater convenience and durability than cotton or foam plugs, with none of the preparation time associated with cotton. The silicone closure has a hydrophobic glass microfiber filter—polyester reinforced for long life. The filter prevents microorganisms and contaminants from entering the culture while allowing free gas/air exchange. Stopper materials withstand 50 autoclave cycles.

14005-20

01929-01

14005-22

Sterile Bioassay Dishes, PS

Large, square dishes feature flat bases for uniform distribution of media. Bioassay dish is ideal for agar diffusion assays or storage use in screening libraries. Growth area is 500 cm2; suggested working volume is 135 mL. Available with or without cell culture treatment. Dishes feature stacking ridges and include lids. Sterilized by irradiation.

Catalog number Description L x W x H (mm) Qty/cs Price/csKH-01929-00 Standard bioassay dish 245 x 245 x 25 16 KH-01930-37 Cell culture treated dish 245 x 245 x 25 16 KH-01929-01 Low profile bioassay dish 234 x 234 x 17 20

06299-00



910


Sterile Disposable Flasks, PETG

l Reduce cross-contamination suspension in cell cultures, media preparation, or storageSterile, disposable Erlenmeyer flasks offered with either a plain or baffled base. Plain bottom is ideal for containing, mixing and storage of solutions; baffled bottom is designed for use on shaker tables. Flasks are graduated for quick volume assessment. Clear polyethylene terephthatlate co-polyester plastic (PETG) with leakproof, polyethylene screw closures.

06112-54

06112-62

Brand 4980 Flasksl Erlenmeyer, narrow mouth, heavy-duty rim, graduatedApproximate graduations are in durable white enamel. An extra large marking label is also provided. Heavy, durable top tooling.

34503-05

34503-04

Cap.(mL)

Stopperno.

Graduationrange (mL)

Graduated(mL)

Catalognumber

Qty/pk

Price/pk

Catalognumber

Qty/cs

Price/cs

10 00 5 to 10 5 — — — EK-34503-21 12 25 0 10 to 25 5 EK-34503-02 12 EK-34503-22 48 50 1 20 to 50 10 EK-34503-03 12 EK-34503-23 48 125 5 50 to 125 25 EK-34503-04 12 EK-34503-24 48 250 6 50 to 200 25 EK-34503-05 12 EK-34503-25 48 300 6 100 to 300 25 EK-34503-06 12 EK-34503-26 48 500 7 100 to 500 50 EK-34503-07 6 EK-34503-27 36

1000 9 250 to 1000 50 EK-34503-08 6 EK-34503-28 24 2000 10 600 to 1800 200 EK-34503-09 1 EK-34503-29 8 4000 10 1000 to 4000 500 EK-34503-10 1 EK-34503-30 4 6000 10 1500 to 6000 500 EK-34503-11 1 EK-34503-31 4

Starter set contains one each of 50 mL, 125 mL, 250 mL, 500 mL, and 1000 mL — — — EK-34503-90 5

Sterile Disposable Flasks, PC

Erlenmeyer, sterile, nonpyrogenic These graduated, wide-mouth flasks are manufactured from optically clear polycarbonate and can be used for cell culture or storing media. Two different styles of caps are offered— a standard two-position polypropylene screw cap or a vented cap that features a hydrophobic membrane to allow gas exchange. The 2000- and 3000-mL flasks are offered with either a flat or baffled bottom; choose a baffled bottom for improved mixing. The 3000-mL flasks are Fernbach style for increased surface area. All flasks are individually wrapped.

Catalog number Capacity (mL) Graduations (mL) Bottom shape Qty/cs Price/csFlasks with two-position screw caps

EK-06114-52 125 25

Flat

50 EK-06114-54 250 25 50 EK-06114-56 500 50 25 EK-06114-58 1000 50 25

Flasks with vented capsEK-06114-60 125 25

Flat

50 EK-06114-62 250 25 50 EK-06114-64 500 50 25 EK-06114-66 1000 50 25 EK-06114-68 2000 50 Flat 6 EK-06114-70 Baffled 6 EK-06114-72 3000 — Flat 4 EK-06114-74 Baffled 4

06114-64

06114-70

Catalognumber

Capacity(mL)

Bottomshape

Qty/pk

Price/pk

EK-06112-60 125

Flat

24 EK-06112-62 250 12 EK-06112-64 500 12 EK-06112-66 1000 6 EK-06112-68 2000 4 EK-06113-50 125

Baffled

24 EK-06113-52 250 12 EK-06113-54 500 12 EK-06113-56 1000 6 EK-06113-58 2000 4



911


Culture Tube Closuresl Closures minimize evaporation while

allowing necessary gas exchangel Positive-grip closures of stainless steel or

autoclavable polypropyleneThese tube closures ensure the viability of microbiological cultures by protecting solid and broth media from excessive evaporation. They fit snugly on rimless culture tubes and flasks while allowing for gas exchange and ventilation.

Stainless Steel Closures

Capacity(mL)

Approximate OD x length

(mm)

Catalognumber

Qty/pk

Price/pk

Catalognumber

Qty/cs

Price/cs

0.5 6 x 50 EK-34570-12 72 EK-34570-32 720 3 10 x 75 EK-34570-01 72 EK-34570-21 720 5 12 x 75 EK-34570-02 72 EK-34570-22 720 9 13 x 100 EK-34570-03 72 EK-34570-23 720 11 16 x 100 EK-34570-04 72 EK-34570-24 576 15 16 x 125 EK-34570-05 72 EK-34570-25 576 20 16 x 150 EK-34570-06 72 EK-34570-26 576 27 18 x 150 EK-34570-07 72 EK-34570-27 576 34 20 x 150 EK-34570-08 72 EK-34570-28 576 50 22 x 175 EK-34570-09 72 EK-34570-29 432 55 25 x 150 EK-34570-10 72 EK-34570-30 288 70 25 x 200 EK-34570-11 48 EK-34570-31 192

34570-10

34570-03

l DisposableHeavy-walled tubes resist breakage. Choose from clear polystyrene (PS) or natural polypropylene (PP). The 12 x 75 mm tubes have a volume of 6 mL; the 17 x 100 mm tubes have a volume of 16 mL. Tubes are sterile; include two-position PE caps.

Brand 9820 Culture TubesThese tubes are rimless for convenient plugging and rack storage. Walls and bottoms are of uniform thickness. Ends are fire polished. The 6-mm, 22-mm, and 25-mm OD tubes do not have marking spots.

Sterile Culture Tubes, PS and PP

Sterile culture tubes come with

two-position caps

PP

PS

Fits tube OD Color Catalog number Qty/cs Price/cs13 mm16 mm16 mm16 mm

White KH-04501-00 1000 Black KH-04501-01 1000 Red KH-04501-02 1000

Yellow KH-04501-03 1000 16 mm16 mm25 mm25 mm

Blue KH-04501-04 1000 White KH-04501-05 1000 Red KH-04501-06 500

Yellow KH-04501-07 500 25 mm25 mm38 mm

White KH-04501-08 500 Natural KH-04501-09 500 White KH-04501-10 250

Kap-Uts™ Polypropylene Closures

Fits tube OD Color Catalog number Qty/cs Price/cs13 mm — KH-04500-00 144 16 mm — KH-04500-01 144 18 mm — KH-04500-02 144 25 mm — KH-04500-03 72 38 mm — KH-04500-04 36

63600-30

06339-90

04501-06 04501-07

04500-04


Disposable Sterile Culture Tubes, PS

These tubes are manufactured from optically clear polystyrene and supplied with threaded plug seal caps to prevent leakage. Untreated tubes have a hydrophobic surface that significantly reduces the attachment of most cells. Treated tubes have a hydrophilic and ionic surface that allows optimal cell attachment. Sterilized by gamma radiation and certified nonpyrogenic.

34534-30Catalog number Surface Capacity (mL) Size (mm) Qty/pk Qty/cs Price/cs

EK-34534-30 Untreated 15 16 x 125 25 500 EK-34534-32 Treated 50

Catalognumber

Size(mm) Material Color Qty/

pkPrice/

pkEK-06339-90 12 x 75 PP Natural 1000 EK-06339-95 12 x 75 PS Clear 1000 EK-63600-20 17 x 100 PP Natural 500 EK-63600-30 17 x 100 PS Clear 500



912


EZ Flip™ Conical Centrifuge TubesLeakproof, one-handed opening and closingl Airtight, leakproof, and sterilel Hinged, flip-cap design for single-handed

manipulation at an affordable pricel Improves ergonomics and versatility without

compromising aseptic conditions or cap loss

Cat. no. Capacity (mL) Tube material Dia x H (mm) Max RCF (x g) Cap included Sterile Packaging Qty/cs Price/csEK-17411-30 15 PP 17 x 120 8500 Yes Yes 10 bags of 50 500 EK-17411-32 10 racks of 50 EK-17411-34 50 PP 30 x 115 9500 Yes Yes 20 bags of 25 500 EK-17411-36 20 racks of 25

Specifications & Ordering Info

Half racks save space—they’re half as long as full-size unwire racks with the same durable, polyketone (PK) construction. Choose from four test tube sizes. Autoclavable.

Unwire™ Half Racks, PK

04501-06

04501-07

Sampling SwabslIdeal for sample collection, cleaning, and microbial analysisl Sterile swabs come individually wrapped

Wood Spatulas are made from close-grained, northern white birch for a smooth, splinter-free finish. Spatulas have no taste or odor— perfect for spreading, stirring, and mixing food samples.

Cotton Swabs feature an oversized cotton tip. Cotton is carded and spun, then bonded securely to a wood shaft.

Rayon Swabs have tips made of the finest USP grade of rayon fiber. They are disposable and come with either a wood or plastic shaft.

Dacron® Swabs are inert and come with either a plastic or wood shaft. Swabs 14001-57 come with reusable transport tubes. Simply place the swabs back in the tube after use, then seal for transport to the lab.

Calcium Alginate Swabs have tips made of organic, biodegradable fiber. Select from plastic or wood shaft.

Inert Dacron swabs with wood

or plastic shaft

14001-52

14001-57

USP grade rayon swabs with wood

or plastic shaft

14001-56

Organic calcium alginate swabs with wood or plastic shaft

14001-40

14001-51


Description Shaft SizeNon-sterile Sterile

Cat. no. Qty/bx Price Cat. no. Qty PriceWood spatulas Wood 6"L x 11⁄16"W KH-14001-50 1000 /bx KH-14001-51 1000 /csCotton swabs Wood 6"L x 1⁄12" dia KH-14001-52 500 /bx — — —

Rayon swabs Wood 6"L x 1⁄12" dia KH-14001-53 1000 /bx KH-14001-55 1000 /csPlastic 6"L x 1⁄10" dia KH-14001-54 1000 /bx KH-14001-56 1000 /cs

Dacron swabs

Plastic 6"L x 1⁄10" dia — — — KH-14001-10 1000 /csWood 6"L x 1⁄12" dia — — — KH-14001-20 1000 /cs

Plastic (in transport tube) 6"L x 1⁄10" dia — — — KH-14001-57 30 /bx

Calcium alginate swabs

Plastic 6"L x 1⁄10" dia — — — KH-14001-30 1000 /csWood 6"L x 1⁄12" dia — — — KH-14001-40 1000 /cs

17411-3417411-30

Catalognumber

Tubesize

Tubes held(array)

DimensionsW x H x D (mm) Color Qty/

pkPrice/

pkEK-06738-50 13 mm 36 (6 x 6) 102 x 56 x 102 Red 1 EK-06738-55 13 mm 36 (6 x 6) 102 x 56 x 102 White 1 EK-06738-60 16 mm 36 (6 x 6) 126 x 68 x 126 Red 1 EK-06738-65 16 mm 36 (6 x 6) 126 x 68 x 126 White 1 EK-06738-70 20 mm 20 (4 x 5 128 x 83 x 103 Red 1 EK-06738-75 20 mm 20 (4 x 5) 128 x 83 x 103 White 1 EK-06738-80 25 mm 16 (4 x 4) 122 x 75 x 122 White 1 EK-06738-83 25 mm 16 (4 x 4) 122 x 75 x 122 Blue 1



913


Nobuto Blood Filter StripsIdeal for blood collection applicationsCollect blood on the narrow end of the strip and place on the drying rack for easy and efficient sample handling. Each strip can hold approximately 0.1 mL of blood or 0.04 mL of serum. Strips are produced with high-purity cellulose paper with a fine, textile like structure—ideal for uniform sampling. Typical blood collection applications are: toxoplasmosis (HA), hanta virus, new castles disease (HI), canine distemper (VN), measles (Rubeola), Japanese encephalitis (HI, VN), mycoplasmosis (AG), and hog cholera (VN). Dried samples can be analyzed, stored, or mailed without refrigeration.

AccessoriesKH-06644-42 Filter strip drying rack

Catalog number Description Qty/pk Price/pkKH-06644-40 Nobuto blood filter strips 100


Nobuto blood filter strip 06644-40 with drying rack 06644-42

DNA Sponge™ Sampling SwabsSterile and individually wrappedSampling swabs are made of urethane foam with a polystyrene (PP) shaft, and are manufactured under the strictest quality controls in ISO 9002 registered facility. Sterile and for single use only. Ideal for wildlife forensic biology, DNA collection, Buccal cell collection, rapid diagnostic, paternity, and evidence collection. The urethane foam swab has been proven to be more effective than cotton swabs for sampling for DNA in blood.

Catalog number Description Package size PriceKH-14001-62 Regular foam tip on 6" PP shaft 100/bx KH-14001-60 1000/cs KH-14001-66 Round foam tip on 5" PP shaft 50/bx KH-14001-64 500/cs


14001-64

14001-60

20610-20

20610-22

Catalog number Description PriceKH-20610-20 Electronic tally counter, add only KH-20610-22 Electronic tally counter, add/subtract

Specifications & Ordering InformationDisplay: 4-digit LCD, 0.35"H Power: AA battery (included)Battery life: 250 days (without sound) Operating temperature: 32 to 122°F (0 to 50°C)

Electronic Tally CountersLarge, 4-digit LCDl Beep at every count with the option of switching the sound off for silent operationl Reset protection prevents accidental reset—hold on/off/reset button for 3 seconds to resetl Long battery life—250 days using silent operationThese counters offer an electronic version of the hand tally counter. Counters are input using large positive-action buttons. Model 20610-20 has a single count button and model 20610-22 has two separate count buttons. The "+" button (green) will add a count to the total and the “–” button (red) will subtract a count from the total. When activated, an audible “beeper” sounds every count to verify that a count has been registered. All electronic components provide a long life counter with no moving parts to wear out. All counters are manufactured from impact-resistant plastic, combining lightweight with outstanding durability.

What’s included: carrying cord and battery.



914


This easy-to-use counter records up to 9999 units. Ideal for multiple applications—use it to count blood cells, bacterial colonies, or food portions. The high-impact plastic case encloses an electronic counter with no mechanical parts to wear out. Features four buttons including ON/OFF, STEP, START, and COUNT keys. Key chain provides portability and easy access. Replaceable button-style 1.5 V battery has an estimated life span of 250 days.


Economical Colony-Counting SystemEnhanced viewing system ensures accurate countsA Handheld Electronic Colony Counter simultaneously marks and counts bacterial colonies; audible signal confirms contact for accurate counts. Selector switch lets you count up or count down to verify totals. Oil- and water-resistant felt-tip marker works on plastic and glass dishes. Counter includes two markers (red and black) and 3 V battery.

B Mini Light Box is ideal for viewing slides, mini-gels, and petri dishes. Operates on four C batteries (not included) or optional 110 VAC adapter. Includes a removable counting grid. Dimensions: 61⁄2"W x 13⁄4"H x 53⁄8"D.

C Mini Magnifier offers 1.75x magnification. Unique stand accommodates mini light box (above) for hands-free magnification. Overall dimensions: 71⁄2"W x 63⁄4"H x 43⁄8"D (lens measures 55⁄8"W x 41⁄8"D).

D Complete Counting System includes counter 14211-02, mini light box 14211-04, 110 VAC adapter 14211-62, and mini magnifier 14211-64 described at above.

A

B

CD

Key Description Catalog number PriceA Handheld electronic colony counter KH-14211-02 B Mini light box KH-14211-04 C Mini magnifier KH-14211-64 D Complete counting system KH-14211-00

Counting system 14211-00

This colony counter increases versatility and operator comfort for quick, accurate microbial counts. Counter features a 120-mm dia, 10-mm deep well with integral pressure sensor—just touch the petri dish with any marker to activate the counting mechanism. Counter confirms each mark with an audible signal.

Counter well accommodates 70-mm and 100-mm petri dishes and features a Wolffhügel grid. Ring-shaped lamp provides uniform illumination in both dark- and light-field modes—select direct illumination for dark nutrient agars, indirect for lighter agars. Flexible-arm 3x magnifier (100-mm dia) lets you position lens for optimal viewing.

Counter also features an input port for optional counter marker 14015-50 to count colonies on plates outside the well.

What’s included: 20 W ring lamp, magnifying lens with flexible arm, and Wolffhügel grid.

Deluxe Colony Counter Adjust pressure sensitivity, illumination, and magnification to your marking style

KH-14015-50 Counter marker with closing contact for external counting

14015-00

Digital counter well accommodates 70-mm and

100-mm dia petri dishes.

Catalog number Description Power Dimensions (W x H x D) PriceKH-14015-00 Deluxe colony counter 110 VAC, 50/60 Hz 113⁄4" x 31⁄2" x 123⁄4" KH-14015-05 220 VAC, 50/60 Hz


Mechanical Tally CountersThe laboratory standard tool for counting bacterial colonies and blood cells or tallying any repetitive event. Four-digit counters feature a quick reset knob and a lens that makes the digits very easy to read. No-fail mechanism and tough, corrosion- proof stainless steel case ensure years of use.

Handheld model 20610-00 has a finger ring for a secure, comfortable grip. Single desk mounted style 20610-12 has plastic base with mounting holes. Multiple desk units 20610-14 mounted on a single base with a common reset.

Cat. no. Description PriceKH-20610-00 Handheld tally counter KH-20610-12 Desk tally counter KH-20610-14 Versa tally, 4 units

Electronic Hand Tally

20610-1520610-00

20610-14

Catalog number Description PriceKH-20610-15 Electronic hand tally

KH-09377-14 Replacement batteries, 1.5 V. Pack of 6

Accessories for colony counter

KH-14211-50 Replacement tip, black KH-14211-54 Replacement tip, red KH-14211-56 Replacement battery, 3 V button cell

Accessories for mini light box

KH-14211-62 Adapter, 110 VAC KH-09376-02 Batteries; 1.5 V, size C. Pack of fourKH-14211-60 Replacement grids. Pack of five



915


E

C

D

Inoculation Loops and Needles

E Insulated Brass Loop and Needle HolderHolder is constructed of durable, heat-resistant brass with insulating sleeve. Works with all gauges of Oxford and Nichrome V loops and needles.

KH-14203-34 Insulated brass holder. Measures 8"L

C Nichrome® V Inoculating Loops

Catalog number Description Inside dia. Handle length and type B&S wire gauge Qty/pk Price/pkKH-14203-24

Loop

2 mm 8", insulated 26 6 KH-14203-23 3 mm 8", insulated 26 6 KH-14203-20 3 mm 6", noninsulated 24 12 KH-14203-22 3 mm 8", noninsulated 25 12 KH-14203-21 4 mm 8", noninsulated 24 12 KH-14203-32 Needle — 6", noninsulated 24 12 KH-14203-33 — 8", insulated 24 6

These loops are manufactured to very close tolerance levels and can be used for semi-quantitative counting of bacteria. Calibrated loops meet APHA standards. Made of 26-gauge wire. Order insulated loop and needle holder for individual loop separately below (“E”).

Ideal for milk and urine cultures. The 1-µL loops are made of 26-ga B&S platinum wire with 15% iridium. The 10-µL loops are calibrated to American Public Health Association (APHA) standards and made of 19-ga B&S platinum wire with 5% rhodium. Order insulated loop and needle holder separately below.

Catalog number Description Loop

lengthContained

volume Price

Nichrome V calibrated inoculating loopsKH-14203-31 No handle 3" 1 µL /eaKH-14203-25 6" insulated

handle 13⁄4" 1 µL /eaKH-14203-26 10 µL /ea

Nichrome V inoculating loopsKH-14203-27

No handle

2 mm — /pk of 12KH-14203-28 3 mm — /pk of 12KH-14203-29 4 mm — /pk of 12KH-14203-30 5 mm — /pk of 12

A Oxford® Platinum Calibrated Loops

F Nunc® Inoculating Loops and NeedlesThese sterile, polystyrene loops and needles are calibrated using a standard urea solution. A Nunc calibration certificate can be obtained upon request. With no need to flame, these loops and needles save you time. Instruments are disposable, eliminating the risk of contamination. The surface of each loop and needle receives a special hydrophilic treatment to enhance the adhesion of droplets and prevent accidental discharge. Loops are color coded for easy identification.

F

Catalog number Contained volume Inside dia Length Price/eaKH-01850-00 1µL 1.45 mm 70 mm KH-01850-02 10µL 4 mm 25 mm

Use for transferring bacterial cultures. These loops and needle are made of B&S platinum wire with 15% iridium added for rigidity, permitting a thinner wire for delicate work. Loops are fused to form a "perfect circle" with an inside tolerance of ±0.02 mm to ensure accurate delivery. Order insulated loop and needle holder separately below.

B Oxford® Platinum Loops and Needle

Catalog number Description Inside dia B&S guage Length Price/eaKH-01850-04

Loop

2 mm24 45 mm

KH-01850-06 3 mm KH-01850-08 5 mm KH-01850-10 3 mm

26

KH-01850-12 3 mm 70 mm KH-01850-14 Needle —

A

B

Ideal for use in semi-quantitative procedures, these loops and needles are 23⁄4"L with 6" or 8" handle. The 24 gauge is the most durable; the 25 gauge is for general use; and 26 gauge is designed for rapid cooling. Choose from insulated or noninsulated aluminum handles.

D Nichrome V Inoculating Loops and Needles with Handles

See next page for more...

Catalog number Description Color Contained volume Qty/pk Price/pkKH-14213-01

LoopBlue 10 µL 600

KH-14213-00 1000 KH-14213-11 Clear 1 µL 600 KH-14213-10 1000 KH-14213-21 Needle Yellow — 600 KH-14213-20 1000



916


Catalognumber Description Fits plate

diameterSpreadingbar width Price

KH-14212-00 Small cell spreader 60 mm 16 mm KH-14212-02 Large cell spreader 90 mm 25 mm

Stainless Steel Cell SpreadersStainless steel cell spreaders have a smooth triangular end with straight spreading bar. Both small and large sizes are 302 mm (8") long. Autoclave or flame sterilize.

G Inoculating Needle and Loop

Flexible polystyrene inoculating device has needle on one end, loop on the other—ideal for streaking bacterial cultures and picking colonies. Sterile and disposable to eliminate cross- contamination. Color coded for easy identification. Case of 1000 (20 per bag, 50 bags per case).

Prevent the risk of cross-contamination with these sterile, disposable needles and loops. Color coded for easy identification. Box of 960 (32 per bag, 30 bags per box).

H Combination Inoculating Needle/Loop

14200-00

06231-series

G H

Oxford® Bacti-Cinerator® IVl Sterilizes platinum loops and needles safely and convenientlyl Prevents infectious spatter and cross contaminationThe Bacti-Cinerator IV utilizes infrared heat to incinerate organic material deep within the ceramic funnel tube. Sterilization takes only 5 to 7 seconds at optimum sterilizing temperature of 1500ºF. Unit can be used in anaerobic chambers. See our selection of inoculation loops and needles on page 915.

KH-01850-26 Replacement heater element for 01850-22 KH-01850-28 Replacement heater element for 01850-24



Disposable Cell SpreadersMade of durable polystyrene, these sterile, disposable spreaders provide safe, convenient sample plating without flaming. L-shaped design offers easy spreading; simply turn petri dish 360º for smooth and even distribution. Slightly upturned end won’t cut into agar.

Catalog number Description Qty/pk Qty/cs Price/csKH-14201-00 Disposable cell spreaders 10 3000 KH-14201-10 1 2000

Inoculating TurntablesProduce evenly distributed bacterial colonies—every time

These hand-operated inoculating turntables produce nearly concentric circles of evenly distributed bacterial colonies from dish edge to center. Deposit your bacterial sample in the center of the petri dish. Rest your spreader on the surface of the agar and gently spin the turntable to distribute the sample into concentric circles. Evenly distributed samples promote identification and ensure uniform exhaustion of agar nutrients.

The inoculating turntables come in two sizes. Model 14212-10 sits flat on the benchtop and accommodates 100- and 150-mm diameter petri dishes. Model 14212-20 comes with a tripod and accommodates 100-mm diameter petri dishes. Both units feature a baked-on acrylic enamel finish with a black enamel weighted base, a raised gripper lip, and a recessed center disc covered with nonskid rubber.

Turntable 14212-2014212-10

Catalog number

DimensionsH x dia

Dish size(mm dia) Price

KH-14212-10 11⁄4" x 6" (30 mm x 150 mm) 100 and 150 KH-14212-20 3" x 41⁄2" (80 mm x 110 mm) 100

01850-22

14212-02

14212-00

See previous page for more...

Catalog number Description Color Dimensions Price/bxKH-06231-11 Needle White 9"L KH-06231-21 1 µL loop Blue 9"L x 1 mm ID loop KH-06231-31 10 µL loop Yellow 9"L x 4 mm ID loop

Catalog number Description Capacity Color Dimensions Price/csKH-14200-00 Needle/loop 10 µL Blue 73⁄4"L x 4 mm ID loop KH-14200-01 Needle/loop 1 µL Clear 73⁄4"L x 1 mm ID loop

Catalog number Description Power

(VAC,Hz) Price

KH-01850-22 Bacti-Cinerator IV 120, 60 KH-01850-24 220, 50/60



917


AccessoriesKH-39458-50 Printer, 115 VAC. Operates on rechargeable battery pack. Includes battery recharger and printer cable KH-39458-52 RS-232 cable for PC output KH-39458-54 Tube adapter for 10- and 12-mm tubes

Wavelength: fixed at 600 nmPhotometric range: 0.3 to 1.99 APhotometric accuracy:

±0.02 A at 1.0 A

Bandwidth: 10 nmOutput: RS-232Power: 115 VAC, 50/60 HzDimensions: 57⁄8"W x 23⁄8"H x 71⁄8"D

Cell Density PhotometerLightweight and portable— fits crowded benchtops or sterile hoodsl Easily measure the density

of cells in suspensionl Take immediate measurements—

no warm-up time requiredl Internal memory can store up

to 99 readingsThis cell density photometer is suitable for measuring the growth rates of many types of cells, including E. coli and yeast. The LED light source works instantaneously allowing measurements to be taken immediately after power-up. The meter detects light at 600 nm and displays results in Absorbance units on the LCD. Rechargeable battery pack provides added convenience for on-the-spot readings minimizing sample transport. Store up to 99 readings or download your data to a PC or printer via the RS-232 interface. Order optional printer or RS-232 cable below.

What’s included: rechargeable battery and external power adapter/recharger for 115 VAC operation.

39458-00

Catalog number Capacity Pathlength Qty/pk Price/pkKH-39458-60 1.5 mL to 3.0 mL

10 mm 100

KH-39458-62 70 µl to 1.8 mL KH-39458-64 70 µl to 2.1 mL

Catalog number Description Price/pkKH-39459-00 Cell density photometer

UV-Transparent Disposable Cuvettes; 10-mm pathlength


Replica-Plating ToolUse this economical device to accurately replicate colonies grown on 100-mm petri dishes. A locking aluminum ring (102 mm dia) secures a sterile velveteen square onto a PVC cylinder for easy control of the contact surface.

To replicate colonies, invert master petri dish over device to transfer colony pattern to fabric, then remove and lower device onto fresh agar plate. Disinfect between uses with a brief rinse in ethanol or chlorine bleach. Cylinder is 55 mm H with 80-mm and 88-mm dia ends; tool includes 12 sterile velveteen squares.

Multi-Grid Replica-Plating UnitUniquely designed unit contains nine of the most popular grid patterns for molecular biology and microbiology experiments— plus one blank sheet for custom patterns—all in one convenient viewing stand.

Stand is slightly angled for optimal viewing and features a locking band to securely hold any 100-mm petri dish. Grid patterns are printed on clear acrylic sheets for viewing on light box. Patterns include 25-, 50-, 70-, and 100-square numbered grids; 150- and 200-square grid; 100-square black grid; and 6- and 12-section circles. Overall dimensions: 4"W x 11⁄2"H x 41⁄2"D. Available in four colors

KH-14002-51 Replacement locking band0.00KH-14002-52 Replacement grid booklet00.00

Catalog number Description PriceKH-14210-00 Replica-plating tool

KH-14210-50 Replacement velveteen squares, 152 mm x 152 mm (6" x 6"). Sterile 100% cotton squares are packed two per bag. Box of 18 bags (36 squares)

14210-00

Full-Range ColorimeterFlexible tool for wide variety of analysesl Excellent for education and basic colorimetry applicationsl Easy output of reaction kinetics This versatile colorimeter is designed for easy wavelength adjustments to span common visible spectrum applications. Eight-filter wheel turns to exhibit desired color-coded wavelength— 440, 470, 490, 520, 550, 580, 590, or 680 nm. Results can be displayed as absorbance units or % transmission. Use the kinetics mode to study rates of reaction and enzyme kinetics—outputs one reading per second.

Measure samples in either standard 10-mm pathlength cuvettes (regular or semi-micro) or in 16-mm diameter test tubes. Large digital display and simple push-button controls. Designed to withstand rugged use and clean up easily. Individual filters are replaceable. Analog and RS-232 outputs enable data transmission to PC, printer, or recorder.

Both models accommodate power sources of 110 to 250 VAC. Model 39459-62 also includes a built-in NiMH rechargeable battery, enabling on-the-spot analysis in any location.


39459-60

Catalog number KH-39459-60 KH-39459-62Operational modes Absorbance, transmission, kinetics

Photometric range Absorbance: –0.3 to 1.99A % transmission: 0 to 199%T

Wavelength range Standard gelatin filters: 440, 470, 490, 520, 550, 580, 590, 680 nmBandwidth 40 nmWavelength accuracy ±2.5 nmRepeatability ±0.02 at 1A using cuvettesOutputs RS-232, 0 to 2 VDimensions 6"W x 21⁄2"H x 7"D

Power 110 to 250 VAC, 50/60 Hz 110 to 250 VAC, 50/60 Hz or rechargable battery

Price

Catalog number Stand color PriceKH-14002-01 Blue KH-14002-02 Red KH-14002-03 Yellow KH-14002-04 Black



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VERIcleenTM Rapid Surface Hygiene Indicator KitDetects invisible food residue without an instrument—in only 10 to 60 secondsVERIcleen is sensitive to food residues including meat, dairy, bakery, beverages, eggs, fish, etc. It is an inexpensive rapid hygiene program for the food service, retail, and institutional industries. Applications include HACCP programs, hand washing hygiene compliance, and overall sanitation monitoring.

Application is extremely simple, just spray to wet dry surface and swab. A “clean surface” shows no purple after three minutes. A “not clean” surface shows purple in 10 to 60 seconds.

What’s included: includes 60 tests and a bottle of wetting liquid with atomizer.

14040-30

Ordering InformationCatalog number Description Contents Price

KH-14040-30 VERIcleen rapid surface hygiene indicator kit 60 tests

Ordering InformationCatalog number Description Contents Price

KH-36152-00 HY-RiSE rapid hygiene test kit 50 tests 36152-00

Hy-LiTE® 2 Luminometer SystemPortable luminometer for hygiene detection in food industryl Test processing surfaces or rinse water controll Automatic temperature compensationl Build-in printerThe Hy-LiTE 2 system is robust, lightweight and fully portable. It detects ATP (adenosine triphosphate), a substance found in all living cells and in most biological material. The luminometer has a large, easy-to-read graphic display and a built-in printer. It can be operated as a test only device, with direct printout of results; as a test and store device, storing up to 2000 results for later printing or for downloading to a PC; or as part of an HACCP Plan using TREND 2 software, a powerful analysis and data presentation package running under Windows 3.1 or Windows 95/NT.

The HY-LiTE® 2 sampling pen is a prepared, one-shot device available in two formats: for surface testing (36153-02) or for rinse water control (36153-04). Each pen is a combined sampling device, test chamber and reagent dispenser in one. Pans contain all the reagents necessary in the correct amounts.

What’s included: Hy-LiTE 2 luminometer, quality assurance certificate and test report, mains power supply and adapters for international use, laminated instruction sheet for correct pen use, lithium activation card, battery holder, four AA alkaline batteries, printed paper roll, TREND 2 Windows Software for PCs, and PC connector cable.

Catalog number Description Qty PriceKH-36153-00 Hy-LiTE 2 Compact kit 1 KH-36153-02 Refill pack with integrated pen 100/pk KH-36153-04 Sampling pens 50/pk KH-36153-10 Paper rolls 1 KH-36153-14 Shoulder bag 1

36153-00

Fireflies and food…Luciferase is an enzyme derived from fireflies that is highly sensitive to the adenosine triphosphate (ATP) present in food residues and in all bacteria, yeast, and mold cells. Bioluminescence tests use luciferase to activate the ATP in samples collected using a specialized swab. The swab emits light in proportion to the amount of ATP in the sample. A luminometer measures that light output, converts it to a numeric value, then stores and displays the results.

HY-RiSE® Rapid Hygiene Test KitFor assessing the cleanliness of surfaces and CIP rinse waterl This easy-to-use, instrument-free kit includes 50 individually wrapped test strips.l Ideal for dry surface testing, hand testing and CIP rinse water testing used in

the food, pharmaceutical, and manufacturing industries. l Use NAD as bioindicator which provides ATP sensitivity but is more cost effective.Application is extremely simple. For dry, smooth surfaces and hand testing, add one drop of wetting solution to the small pad on the end of the strip. For liquid testing, swipe the testing area with the wetted pad side; no need for reagent. To test the CIP rinse water, dip the padded end into the sample and then add substrate solution immediately followed by one drop of enzyme solution. After four to five minutes of reaction, any visible discoloration means that there are residues present and further cleaning is necessary.



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Hygiene Monitoring SwabCheck KitsMonitor and control microbiological contamination of surface areas in the food industryl Excellent for production plants and laboratoriesl Qualitative and semi-quantitative hygiene controll Sterile packed—ready for immediate use

These kits are very easy to use! The surface is wiped with a cellulose swab and any bacteria collected is transferred into a tube containing a special medium with indicator dye and then incubated. Even a single bacterium is sufficient to cause a color change meaning that SwabChecks are about 1000 times more sensitive than the conventional ATP method. This accuracy is particularly important in the food industry. With this simple method, it is possible to identify microorganisms such as Listeria monocytogenes which must not be present at any concentration in food or beverages.

Listeria SwabCheck KitDesigned to be used alongside traditional selective methods to improve the quality system and minimize the risk of Listeria contamination. This simple-to-use diagnostic test can be applied anywhere in the environment and on foodstuffs where the presence of Listeria species would be critical. The test works on an enhanced Esculin media formulation: the hydrolysis of Esculin produces in a distinctive black/brown precipitate. Each kit includes a swab and culture medium.

E. coli SwabCheck KitUsed for the detection of E. coli on surfaces. The visual indicator of fluorescence using a long-wave UV light source confirms the presence of E. coli, and any further confirmation is not required. MUG detects anaerogenic strains that may not be detected in a conventional procedure. This kit detects the existence of ß-glucuronidase, possessed by most E. coli and a few strains of Salmonella, Shigella, and Yersinia. The presence of E. coli is detected by the appearance of fluorescence throughout the tube. Each kit includes a swab and culture medium.

Yeast and Mold SwabCheck KitUsed for the enumeration of yeast and molds on surfaces in accordance with HACCP guidelines. M-Green yeast and mold is an improved modification of the liquid medium, m-yeast and mold broth, and was developed to improve efficiency of detection and enumeration of fungi in sugar-based drinks using the membrane filtration method. The result is very easy to interpret: green opaque colonies against a yellow background are indicative of the growth of yeast. Mold colonies are green and filamentous. Each kit includes swabs and culture medium, packaged with a membrane device, providing a quantitative result.

14019-2014019-1014019-00

Ordering InformationCatalog number Description Qty/pk Price/pk

KH-14019-00 Listeria SwabCheck kit 25 KH-14019-10 E. coli SwabCheck kit 125 KH-14019-20 Yeast and mold SwabCheck kit 30

Rapid Bacterial Test KitsFast and reliable tests for detecting and identifying bacteriaThese test kits provide a quick and easy way to determine the presence of different types of bacteria in food samples, environmental samples and sample colonies. All tests kits are simple to use and produce easy to identify positive or negative results. Kits contain everything to test cultured bacterial colonies.

Cat. no. Tests for Description Includes Price

KH-36151-00 Salmonella Uses anti-Salmonella antibody as a visual indicator for positive ID band on assay strip. 20 tests

KH-36151-02 Salmonella Uses latex particles sensitive to Salmonella. Agglutination occurs when Salmonella are present. 30 tests

KH-36151-04 E. Coli 0157 Uses anti-E.coli 0157 :H7 antibody as a visual indicator for positive ID band on assay strip. 20 tests

KH-36151-08 ListeriaUses simple 20 minute EIA color change for positive result. Positive and negative controls included. AOAC approved method for Listeria spp. in foods.

50 tests

KH-36151-10 StaphylococcusUses rabbit plasma as coagulase to identify pathogenic Staphylococcus species. Clotting signifies positive result after 1 to 4 hours incubation.

6 x 3 mL coagulase

KH-36151-12 Staphylococcus aureus

Uses plasma coated latex beads that react with Clumping Factor and Protein A found in Staphylococcus aureus. Test takes less than 1 minute for positive ID.

50 tests

KH-36151-14 Indole reactionUses Kovacs tube method. Indole combines with tryptophan in the reagent to produce a pink to red color for positive ID.

15 mL reagent

KH-36151-16 Cytochrome oxidase activity

Uses OxiDrops, oxidase reagent to test for oxidase activity. Place one drop on suspect colony directly on the plate. Positives turn purple.

15 mL reagent

KH-36151-18 Catalase reaction Uses a hydrogen peroxide reagent to identify and differentiate catalase-positive and negative colonies.

15 mL reagent

Presence of E. Coli 0157 is confirmed.

Purple indicates cytochrome oxidase

activity.



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36152-4036152-30

36152-2036152-60

Specially adapted enrichment media guarantees precise and reliable test results.

36152-70

No band next to the T is a negative result indicating absence

of the pathogen. (C = control band)

A band next to the T is a positive result

indicating presence of the pathogen.

(C = control band)

Specification and Ordering Information for all rapid pathogen test kits

Listeria test

Campylobacter test

Catalog number Procedure Description Contents PriceKH-36152-72

EnrichmentBuffered peptone water (BPW) 500 g

KH-36152-74 Salmonella enrichment broth according to Rappaport-Vassiliadis (RVS) 500 g

KH-36152-76 Isolation media RAMBACH agar 4 x 1 L KH-36152-70 Confirmation Salmonella test chip 25 tests

Salmonella test

E. coli 0157 test

Verotoxin-producing E. coli test

Rapid Pathogen Test KitsLateral flow tests designed for the rapid detection of food pathogensl Rapidly and reliably confirms the presence of five important

pathogens in foodl Definite test results with an additional positive controlThese rapid pathogen test kits use gold labeled immunoSorbent assay for the qualitative detection of bacteria in food samples. No incubation is required after sample enrichment; no equipment to buy or maintain. Simply add sample and read off the result in just 20 minutes. You’ll get a distinct positive or negative test results with a built-in positive control. As sensitive as the classical culture media methods. Exceeds the performance criteria of USDA-FSIS.

Order enrichment broths, isolation media, and confirmation test chips separately below.

Catalog number Procedure Description Contents PriceKH-36152-62 Enrichment Bolton broth 500 g KH-36152-64 Bolton broth selective supplement 16 vials KH-36152-66 Isolation media Campylobacter blood-free selective agar base 500 g KH-36152-68 CCDA selective supplement 16 vials KH-36152-60 Confirmation Campylobacter test chip 25 tests

Catalog number Procedure Description Contents PriceKH-36152-22 Enrichment mEC selective broth with Novobiocin 500 g KH-36152-24 mTSB selective broth with Novobiocin 500 g KH-36152-26 Isolation media SMAC agar 500 g KH-36152-28 CT-supplement 16 vials KH-36152-20 Confirmation E. coli 0157 test chip 25 tests


Enrichment

mEC selective broth with Novobiocin 500 g KH-36152-24 mTSB selective broth with Novobiocin 500 g KH-36152-32 CAYE broth mod. according to Evans 100 g KH-36152-34 CAYE broth supplement (Carbadox) 16 vials KH-36152-26 Isolation media SMAC agar 500 g KH-36152-28 CT-supplement 16 vials KH-36152-30 Confirmation Verotoxins test chip 25 tests


EnrichmentListeria enrichment broth 500 g

KH-36152-44 Listeria selective enrichment supplement 16 vials KH-36152-46 FRASER Listeria selective enrichment broth 500 g KH-36152-48

Isolation media

FRASER Listeria supplement 16 vials KH-36152-50 OXFORD Listeria agar 500 g KH-36152-52 OXFORD Listeria selective supplement 13 vials KH-36152-54 Palcam Listeria agar according to van Netten et al. 500 g KH-36152-56 Palcam Listeria selective supplement 16 vials KH-36152-40 Confirmation Listeria test chip 25 tests



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14006-00

Microbial Air SamplerCost-effective sampler for monitoring airborne bacteria, viruses, molds, and fungi

AccessoriesKH-14006-50 Contact plate, 65 x 15 mm. Case of 500KH-33687-52 Petri head adapter for 100 x 15 mm petri plates KH-33687-50 Tripod; includes adapter for quick release of air sampler

SAS Super 100 Digital Microbial Air Samplers


Catalog number Description Dimensions Power PriceKH-14006-00 Microbial air sampler 43⁄8"W x 111⁄4"H x 61⁄8"D 110 VAC

Use this air sampler to periodically monitor levels of airborne microbes in processing facilities and lab areas. Impaction-style sampler features a self-regulating drive that provides constant 100 L/min aspiration. Airflow programmable from 10 to 1800 liters of air, defaults to last volume sampled. Specially designed intake holes create laminar air flow. Airborne microorganisms are driven onto the surface of a contact plate (sold separately) for incubation and enumeration; order contact plate 14006-50 separately below. Sampler features low-battery indicator, ergonomic handle, and a shatter-proof stainless steel head.

What’s included: a rechargeable battery pack with power supply.

Set parameters and view readings on the easy-to-read digital display; unit retains previous sample volume. Sample 45,000 liters of air per battery charge. Microbes are driven onto the surface of contact plate for incubation and analysis (order contact plate 14006-50 separately below).

Air sampler with stainless steel head is ideal for cleanroom, pharmaceutical, and electronics applications; meets material requirements for cleanrooms. Includes a sturdy aluminum carrying/transport case.

Air sampler with aluminum head is ideal for food testing and processing applications; includes a soft-sided carrying case.

What’s included: a rechargeable NiMH battery pack, battery charger, carrying case, and integrated RS-232 port for downloading data.

Sampler with stainless steel head 33687-02

Impaction-style SAS air samplers let you accurately collect and quantify airborne bacteria, viruses, molds, and fungil For cleanrooms and process areas in pharmaceutical,

electronics, and food technical environmentsl Choose from eight preset sample volumes (10, 20, 30, 50,

100, 200, 500, or 1000 liters) or program up to eight custom sample volumes

l Detachable metal cover with specially designed intake holes creates laminar air flow; flow rate is 100 L/min

Specifications & Ordering Information Cat. no. Head material Dimensions Power Price

KH-33687-02 Stainless steel 123⁄8"W x 41⁄8"H x 41⁄4"D 115 VAC KH-33687-12 Aluminum

Readycult® Coliforms TestsFor the rapid visual determination of coliforms and E. coli in water samples

36152-10Specifications & Ordering Information

Accessories

Using the Readycult identification method, you will save both time and expense by avoiding the traditional Coliform & E. coli testing methods. You'll also be able to respond more quickly to potentially dangerous situations when Coliforms and E. coli are found to be present in your water samples. As an added bonus, you'll never have to worry about product integrity because the resealable packs do not require refrigeration and have a shelf life of 3 years (post manufacturing date).

Application is extremely simple. Indole test 36152-18 can be directly performed in the same broth for E. Coli confirmation.

l EPA-approved testing protocoll Rapid, one-step method with a 24-hour incubation response time for total coliform presence l A quick second step for confirmation of the presence of E. coli

Catalog number Description Qty PriceKH- 36152-14 Sterile sample bottle, 150 mL 345/cs KH- 36152-16 Sterile sample bottle with dechilorinating agent 345/cs KH- 36152-18 Indole test 30-mL dropper bottle

KH-14006-50 Contact plate, 65 x 15 mm. Case of 500

Catalog number Description Quantity/pk Price/pkKH-36152-10 Readycult Coliforms test 20 snap packs KH-36152-12 200 snap packs



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The MAS 100 was developed on the Andersen Air Sampler Principle with collection apertures that correspond to Andersen Level 5 for excellent recovery of viable particles as small as 1 µm. All models include a built-in calibration reminder that can be set between 1 and 12 months, and calibration is made easy with the digital anemometer 39182-82 (sold separately) which work with both the MAS100Eco and MAS100 Air Sampler. Internal software is easily accessed and controlled using single touch “yes or no” responses and pre-programmed volumes ensure reproducible results.

Air flow is 100 liters/minute. The unit and lid cover are both made of anodized aluminum; lids are autoclavable. Power is supplied via NiMH rechargeable batteries. The display is 16-digit, alphanumeric LCD with real time and date.

MAS 100Eco™ Air Samplerl Microbiological air sampler designed for the

food and beverage industry

l Lightweight unit offers an easy, low-cost method for air monitoring

l Handle doubles as a stand to allow different angles for testingThere are 6 preset volumes: 10, 20, 50, 100, 200, and 500 liters. Users can also reset any of the presets to any volume between 0 to 1000 liters. Run time for rechargeable NiMH batteries is 3 hours and maximum time for complete recharge is 9 hours.

MAS 100 Air Samplerl Samples up to 45,000 liters of air or 7.5 hours before battery

needs to be recharged This model offers a built-in anemometer which compensates for different sampling heads, changes in temperature/pressure, and variably filled petri dishes. Collection volumes of 50, 100, 250, 500, and 1000 liters are preset for selection and can be deactivated or reactivated as needed. A specific volume between 0 and 2000 liters can also be selected.

MAS 100 Air SamplersResults you can trust in monitoring microbial air qualityl Programmable sampling delay up to 59 minutes—

eliminates remote control devices l Use standard 100 mm Petri dishes

Specification & Ordering InformationCatalog number Description Price

KH-39152-80 MAS-100Eco air sampler KH-39182-90 MAS-100 air sampler KH-39182-82 DA-100 Digital anemometer for on site calibration

Optional Accessories

MAS 100Eco AIR SAMPLER

39152-80

MAS 100 Air Sampler

39182-90

MAS 100 AIR SAMPLER 39182-90 with included carrying case

Catalog number Description PriceKH-39182-84 Replacement aluminum preforated lid KH-39182-86 Replacement dustcover KH-39182-92 Battery charger, 110 to 240 VAC KH-39182-94 Replacement battery pack KH-39182-88 MAS-100Eco tripod adapter KH-39182-96 MAS-100 tripod adapter

Call us now to place your order!

847-549-7600

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