CyberColloids SeaRefinery Project 2019...CyberColloids Ltd, Unit 4A Site 13, Carrigaline Industrial...
Transcript of CyberColloids SeaRefinery Project 2019...CyberColloids Ltd, Unit 4A Site 13, Carrigaline Industrial...
CyberColloids Ltd, Unit 4A Site 13, Carrigaline Industrial Estate, Carrigaline, County Cork, Ireland. tel: +353-21-4375773 www.cybercolloids.net
TheSeaRefineryProject CyberColloidsJanuary2019
2019
TheSeaweedBiorefinery–forhighvalue-addedproducts• 3yearproject(November2015–October2018)• Budget~2.6millionEuros(~1.4millionEurogranted)• Consortiumof8partnersfromfiveEUcountries,
includingRTDsandSMEsandsupportfrom1largeenterprise
• FundedundertheMarineBiotechnologyEra-Netbyco-fundingagencies:
www.searefinery.eu
Theconsortium
• developanintegratedbiorefineryprocess• usingeco-friendlychemicalandenzymaticstep-wise
extractionandpurificationsteps• toproducehighvalue-addedcomponentsfromcultivated
brownseaweed(Sccaharinalatissima,Alariaesculenta)• includingantioxidants(phlorotannins),hydrocolloids
(alginate),andotherbioactives(laminarin,fucoidanandprotein)
• toevaluatetheseextractedcomponentsforuseinneutraceutical&functionalfoodingredients,cosmetic&medicalproductsandbiobasedmaterials
• demonstratethebiorefineryconceptatpilotscale
Projectaims
Theworkplan
CyberColloids’role
• labscaleextractionprocessesforpolyphenols,fucoidanandlaminarin
• labscaleprocessestoextractalginatefromwholeseaweedbiomassandfromextractionresidues
• labscaleprocessesfortheproductionofalginaterichseaweedfibresfromwholeseaweedbiomassandfromextractionresidues
• seasonalquantificationofalginatecontentinseaweeds• conceptforSeaRefinerymulti-streamextractionprocess• transferanddemonstrationofSeaRefineryatpilotscale
(600kg)
Labscaleextractionprocess
• processbasedonuseoffresh(orfrozen)seaweed• dryingtemperatures>400Cimpactonyieldsandefficacy• mildaqueousextractionprocessesdevelopedfor
polyphenols,laminarinandfucoidan
• NB:polyphenollevelsinkelpspecieslow(<2%inthiscase)
• typicalyieldsforsequentialextractionofIrishSaccharinalatissimagivenbelow(as%dryweightofstartingmaterial)
Starting seaweed 100Laminarin rich 51.6 ± 3.91Fucoidan rich 4.26 ± 0.45
Alginic acid 15.22 ± 2.94Residual insolubles 16.69 ± 1.78Soluble and losses 3.74 ± 1.05
Scaleupto600kgs
Top Driedstartingmaterial
Aqueousextractioninprogress Sampling
Middle
Solidfractionpostdecanting Loadingsolidsintoreactionvessel Aqueousextractioninprogress
Bottom
Liquidextractduringcentrifugation Separatingacidicsolids
Alginicacidrichseaweedfibre
Alginaterichseaweedfibres
• processesdevelopedforalginicacidrichandsodiumalginaterichseaweedfibres
• holisticprocessusingwholeseaweedbiomass• alginatefunctionalitypromotedinsitu–without
extraction• functionalityoffibresassessedvscommercial
comparators
Alginicacidrichfibres
• Waterbindingcapacity(WBC)assessed• fromacommercialperspective,newtextureingredients
withaWBCofgreaterthan10g/gpotentiallyinteresting• WBCofgreaterthan15g/gispreferable• alginicacidrichfibresfromAlariaandSaccharinahadWBC
of13g/gand18g/g,respectively• inrangewithcommerciallyavailablefunctionalisedfibre
products–seebelow• seaweed=redbars
Sodiumalginaterichfibres
• viscosityandgelstrengthassessed• viscosityofseaweedfibreswas656mPa.S(at1%)• typically,commercialalginatesusedinfoodgiveviscosityof
400-600(-1000)mPa.S(at1%)• Gelstrengthisrepresentedbytheforceingthatisrequired
tobreakthegel• thebreakstrengthfortheSaccharina sodiumalginatefibre
was426g• breakstrengthofcommercialcontrolswas314g–823g• Na alginate seaweed fibres have potential to replace Na
alginateona1:1basiswhichiscommerciallyinteresting
Applicationoffibres
• Na alginate seaweed fibres successfully used at 4-6% toproduce edible films – possibility to replaceNa alginate incasings
• Na alginate fibres successfully used as gelling agents inrestructuredvegetableandfishproducts
• seaweedfibresoutperformedtheNaalginatecontrols• Seaweedfibresusedintwodifferentfacemaskformulations
toreduce/replaceNaalginate
Keyoutcomes
• robust processes for lab scale biorefinery of Saccharinalatissima&Alariaesculenta
• multistreamextractionoffucoidan,laminarin&alginate• polyphenollevelstoolowtobeofinterestinthisproject• demonstratedtransferofbiorefinerytopilotscale(600kgs)• development of alginate rich seaweed fibres with
commerciallyinterestingproperties• demonstrated functionality in targeted food and cosmetics
andapplications