COUNTRY REPORT - OIE · 1/1/2020 · Epidemiology of Classical Swine Fever •The first report...
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1 COUNTRY REPORT Regional Workshop on Swine Disease Diagnosis Beijing, P. R. China, 30 – 31 Oct 2019 The major swine diseases in Thailand and current diagnostic methods
Transcript of COUNTRY REPORT - OIE · 1/1/2020 · Epidemiology of Classical Swine Fever •The first report...
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COUNTRY REPORT
Regional Workshop on Swine Disease Diagnosis
Beijing, P. R. China, 30 – 31 Oct 2019
The major swine diseases in Thailand and current diagnostic methods
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Basic information
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Basic information
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Basic information
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Basic information
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Basic information
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Basic information
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Basic information
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Basic information
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Updates on disease situation
• Situation of major swine disease
o Porcine Reproductive and Respiratory Syndrome (PRRS)
o Classical Swine Fever (CSF)
o Porcine Epidemic Diarrhea (PED)
o African Swine Fever (ASF)
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Porcine Reproductive and Respiratory Syndrome (PRRS)
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• Seropositive animals could be traced back to as early as 1989.
• In 1996, the first Thai PRRSV was isolated and was identified as Type 2 PRRSV (Damrongwatanapokin, S. et al 1996)
• A few years later, Type 1 PRRSV was also reported in Thailand, and some was found to be co-circulating with Type 2 PRRSV within the same herd.
(Thanawongnuwech, R. et al 2004)
Epidemiology of PRRS in Thailand
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Type 1 Type 2
EU NA
Tun, et al. 2011
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Porcine Reproductive and Respiratory Syndromes
Genotype Clade/Lineage
Type 1 (European) Clade A, Clade D, Clade H
Type 2 (North America) L1, L5, L8, Orphanage Lineage
*Classification of PRRS isolate based on Shi et al. (2010) and Tun et al. (2011).
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Laboratory diagnosis• Multiplex qRT-PCR
• PRRSV primers and probes sequence
➢ Eu Fw 5’ GAT GAC RTC CGG CAY C 3’
➢ Eu Rev 5’ CAG TTC CTG CGC CTT GAT 3’
➢ Eu probe 5’ Fam-TGC AAT CGA TCC AGA CGG CTT-BHQ1
➢ NA_Fw 5' ATR ATG RGC TGG CAT TC 3'
➢ NA_Rev 5' ACA CGG TCG CCC TAA TTG 3'
➢ NA_probe Hex-TGT GGT GAA TGG CAC TGA TTG ACA-BHQ1
Kleiboeker SB, 2005
➢ CN_Fw 5’CCC AAG CTG ATG ACA CCT TTG3’
➢ CN_Rev 5’AAT CCA GAG GCT CAT CCT GGT3’
➢ CN_Probe Texas red-5’CGC GTA GAA CTG TGA CAA CAACGC TGA3’-BHQ2
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Laboratory diagnosis
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Classical Swine Fever (CSF)
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Epidemiology of Classical Swine Fever
• The first report case in Thailand occurred in 1950 in Bang ken district, Bangkok.
• Thailand genogroup.
1. Genotype 1 ( subgenotype 1.1 and 1.3)
2. Genotype 2 (subgenotype 2.1 and 2.2)
3. Genotype 3 ((subgenotype 3.3)
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Classical Swine Fever (CSF)
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Genogroup Period
1.1 1988-present
1.3 1988-1993
2.1 2001- present
2.2 1996-2008
3.3 1991-1996
Classical Swine Fever
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Porcine Epidemic Diarrhea (PED)
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Classical Swine Fever (CSF)
NPLAIPMA
Classical Swine Fever (CSF)
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Classical Swine Fever (CSF)
Immunoperoxidase monolayer assay (IPMA)
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Classical Swine Fever (CSF)
Immunoperoxidase monolayer assay (IPMA)
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Porcine Epidemic Diarrhea (PED)
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Porcine Epidemic Diarrhea
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Laboratory diagnosis
❑Real time PCR
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Primer and Probe
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African Swine Fever (ASF)
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Type of sample
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Porcine Epidemic Diarrhea (PED)
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Porcine Epidemic Diarrhea (PED)
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Porcine Epidemic Diarrhea (PED)
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Porcine Epidemic Diarrhea (PED)
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African Swine Fever (ASF)
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African Swine Fever (ASF)
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African Swine Fever (ASF)
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What worked well
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Proficiency testing for swine diseases
Supported by FAO
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Proficiency testing for ASF
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Challenges and possible solution
• Lack of swine sample.
• Lack of collaboration between pig farmers and
government agency.
• Have a good collaboration with laboratory
network.
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Thank you
