CommonCommon--use Data System for Food use Data System for Food Animal Genetics in TaiwanAnimal Genetics in Taiwan

MingMing--Che WuChe Wu

Taiwan Livestock Research InstituteTaiwan Livestock Research Institute

Food security and mileage (Sustainability)Food security and mileage (Sustainability)

A reservoir of genetic adaptability acts a buffer A reservoir of genetic adaptability acts a buffer against potentially harmful environmental and against potentially harmful environmental and economic change.economic change.

System for Food Animal Genetics System for Food Animal Genetics in Taiwanin Taiwan

●● Conservation farmsConservation farms

●● Web siteWeb site www.angrin.tlri.gov.twwww.angrin.tlri.gov.tw

●● CryopreservationCryopreservation Bank Bank

●● Molecular techniquesMolecular techniques

●●Genetic resources Genetic resources exchangeexchange

Phase I (1987~1992)Phase I (1987~1992)：：Conservation farmsConservation farms

Phase II(1993~1998)Phase II(1993~1998)：：Web siteWeb site

Phase III(1999~2004)Phase III(1999~2004)：：Conservation centerConservation center

Phase IV(2005~2008)Phase IV(2005~2008)：：Research and UtilizationResearch and Utilization

Phase V(2009~2012):Phase V(2009~2012):Global Global BioidentificationBioidentification SystemSystem

CommonCommon--useuse•• DNA in food chainDNA in food chain•• Biogenetic banks in Biogenetic banks in

supporting scientific supporting scientific researchresearch

•• Genomic selection on Genomic selection on farm levelfarm level

•• WebWeb--based barcode based barcode system with food mileagesystem with food mileage

乳乳 MilkMilk

Egg Egg 蛋蛋

Meat Meat 肉肉DNA in food chainDNA in food chain

Origin Origin assuranceassurance-- market to farmmarket to farm

Web site: www.angrin.tlri.gov.tw

ConservationConservation

SelectionSelection

Genomic breedingGenomic breeding

SpeciesSpecies

ExportExport

BiodiversityBiodiversity

TrackingTracking

DemandDemandValue addValue add

Species Species verificationverification

New New breed/linebreed/line

Genetic Genetic improvementimprovement

BioUtilizationBioUtilization

(source:FAO, 2007)(source:FAO, 2007)

(milk)(milk)

(milk)(milk)(milk)(milk)

(pork)(pork)(pork)(pork)

(pork)(pork)

(chicken meat)(chicken meat)(chicken egg)(chicken egg)

Global Movement of Global Movement of AnGRAnGR

(fur sheep)(fur sheep)

Biogenetic resources are parts of our life.Biogenetic resources are parts of our life.

From Farm to DNA BankFrom Farm to DNA Bank

Genetic diversity is threatened by Genetic diversity is threatened by -- genetic erosiongenetic erosion-- habitat losshabitat loss-- social value sinkingsocial value sinking-- economic setbackeconomic setback-- food print mileagefood print mileage

Genetic Genetic BankingBanking

Living formLiving formCell/tissueCell/tissue

DNA/RNA/mRNA/DNA/RNA/mRNA/cDNA/proteincDNA/protein

Lanyu

Taiwan

Development of miniature pig for biomedical research in TaiwanDevelopment of miniature pig for biomedical research in TaiwanMing-Che Wu, PhDTaiwan Livestock Research Institutewww.angrin.tlri.gov.twmcwu@mail.tlri.gov.tw

Hybrid

Hybrid

InbreedingInbreeding

LanyuLanyu 200200

Spotty Lanyu(Lanyu 100)

Mitsae (Lanyu 50)

DurocDuroc

GPI-CRC-PGD Genotyped Line (Lanyu 300) (50% Lanyu + 50% Duroc)

Development of miniature pig for biomedical research in TaiwanDevelopment of miniature pig for biomedical research in TaiwanMing-Che Wu, PhDTaiwan Livestock Research Institutewww.angrin.tlri.gov.twmcwu@mail.tlri.gov.tw

19891990

1991

1992

1993

1994

1995((LanyuLanyu 5050)

MitasiMitasi minipigminipig was developed from sibwas developed from sib--mating on hybrid of mating on hybrid of DurocDurocand and LanyuLanyu breed.breed.

19

FullsibFullsib Mating on Mating on coat color selectioncoat color selection

Conservation to BioConservation to Bio--utilization (1987utilization (1987--2008)2008)

LanyuLanyu WhiteWhite20052005

LanyuLanyuBlackBlack19801980

Spotty Spotty LanyuLanyu19931993

Development of miniature pig for biomedical research in TaiwanDevelopment of miniature pig for biomedical research in TaiwanMing-Che Wu, PhDTaiwan Livestock Research Institutewww.angrin.tlri.gov.twmcwu@mail.tlri.gov.tw

Lanyu 300Lanyu 50Lanyu 100

Lanyu 200

Lanyu 100Lanyu 50

Lanyu 300Lanyu 100Lanyu 400

Ex situ conservation of minipig

Semen collection and storage

TTaiwan aiwan AAnimal nimal GGermplasm ermplasm CCenterenterhttp://www.angrin.tlri.gov.tw/http://www.angrin.tlri.gov.tw/

•• Cell BankCell Bank-- Sperm bank, Sperm bank, OocyteOocyte bank, Embryo bankbank, Embryo bank

•• DNA BankDNA Bank

TTaiwan aiwan AAnimal nimal GGermplasm ermplasm CCenterenterhttp://www.angrin.tlri.gov.tw/http://www.angrin.tlri.gov.tw/

Cryotanks: 24K*5 + 38K*17 + 80K*2 = 926K(CryoArk for Taiwan: 46,000 sp. in total)

CryoArk for Taiwan

OocyteOocyteSpermSperm

EmbryoEmbryo

Cell BankCell Bank

Small LN Tank Small LN Tank

--8080℃℃ Freezer Freezer

--185185℃℃ Vapor Vapor storagestorage

•K Series - Liquid nitrogen storage with controllable temperatures between (-100°C to -196°C)

•designed for large vial capacities up to 6000 vials (2 ml vial) in box type racks.

--185185℃℃ Vapor Vapor storagestorage

•designed for large vial capacities up to 6000 vials (2 ml vial) in box type racks.

BioBio--securitysecurity

Cattle 6180Water Buffalo 58Goat 3127Sheep 14Horse 14Rabbit 88Swine 25129Chicken 11650Muscovy 151Duck 689Hybrid Duck 128Goose 431Turkey 14Black Swan 135Quail 50Total 47858

DNA Banking 2007

Gene BankingGene BankingLiving form/Cell/TissueLiving form/Cell/Tissue

DNA/RNA/mRNA/cDNA/ProteinDNA/RNA/mRNA/cDNA/Protein

OocyteOocyte

EmbryoEmbryo

LandraceLandrace Taoyuan

Berkshire

Meishing

Mitsae

YorkshireYorkshire

DurocDuroc

Hampshire

Lanyu

Lee-Sung TLRI Black

LYLY

LYDLYD HogHog

Spotty

Inbreeding

Pig BreedingPig Breeding

2.00

2.05

2.10

2.15

2.20

2000 2001 2002 2003 2004 2005 2006 2007

Duroc Landrace Yorkshire

Birth year of boar

Feed efficiency(Feed/Gain) from 40 to 110 kg of body weight

Impact of commonImpact of common--use breeding data on production efficiencyuse breeding data on production efficiency

Growth performance test for breeding pigGrowth performance test for breeding pig

AuctionAuction

Elite animal labeled with the breeder name Elite animal labeled with the breeder name prior to auctionprior to auction

Performance dataPerformance data of individual pig with its own of individual pig with its own pedigreepedigree along with along with genotypegenotypeinformationinformation

Auction price of each pig and buyer soon after soldAuction price of each pig and buyer soon after sold

OnOn--line Statistics for Auction Data of Elite Pig line Statistics for Auction Data of Elite Pig

Price (NT$)AuctionYear No. of Breeder and Buyer

Cow Milk Production in Dairy Herd Improvement System Cow Milk Production in Dairy Herd Improvement System

Regional Statistics on Dairy Farming SectorRegional Statistics on Dairy Farming Sector

County No. of Farmers Cow Milking Performance

Profiles of daily milk yield per cow in average and number of DHProfiles of daily milk yield per cow in average and number of DHI farms I farms (1990 ~ 2008) (1990 ~ 2008)

15

16

17

18

19

20

21

22

23

24

25

1999

0719

9911

2000

0320

0007

2000

1120

0103

2001

0720

0111

2002

0320

0207

2002

1120

0303

2003

0720

0311

2004

0320

0407

2004

1120

0503

2005

0720

0511

2006

0320

0607

2006

1120

0703

2007

0720

0711

2008

03

100

150

200

250

300

350

400

Daily Milk Yield (kg)DHI Farm (N)

11.21.41.6

1.82

2.22.42.62.8

33.2

3.43.63.8

4

22.07

22.14

22.32

22.3722

.3922.5922.7722

.86 23.1

23.15

23.39

23.45

23.52

23.55

23.76

23.81

24.0424.1124.2424

.3624

.6224

.6924

.7924

.9225

.0725

.1725

.2425.5225.9726.2826.5126

.5727

.2827

.4227

.7127

.7428

.0529.230.1634.51

2.6

2.8

3

3.2

3.4

3.6

3.8

4

4.2

4.4

4.6

Protein % Protein % (milk yield adjusted) Profile on milk quality in Profile on milk quality in protein percentage from protein percentage from those farms having more those farms having more than 22 kg of milk yield than 22 kg of milk yield recorded in 2007.recorded in 2007.

Regulation for genomic breeding Regulation for genomic breeding -- Animal Industry Act Animal Industry Act Article 17Article 17The competent authority may dispatch inspector to examine or tesThe competent authority may dispatch inspector to examine or test t the breeding flock, breeding stock, facilities, pedigree registry and related recorbreeding stock, facilities, pedigree registry and related records of breeders, and ds of breeders, and the breeders shall not evade, interfere with or object to such ethe breeders shall not evade, interfere with or object to such examination or testing.xamination or testing.Breeding flock or breeding stock found to have contracted notifiBreeding flock or breeding stock found to have contracted notified disease or have ed disease or have hereditary diseasehereditary disease during the aforesaid examination or testing shall be during the aforesaid examination or testing shall be banned from from breeding. The aforesaid inspectors shall present their identificbreeding. The aforesaid inspectors shall present their identification when carrying out ation when carrying out their duty.their duty.

PigPig CowCow GoatGoat ChickenChicken DuckDuck GooseGoose DeerDeerPRLPRLESRESRZWZWmtDNAmtDNA

IGF2IGF2XYXYD5FADD5FADmtDNAmtDNA

RabbitRabbit

GeneGeneHalHal--18431843ESRESRHFABPHFABP--HHHFABPHFABP--adadIGF2IGF2--In3In3IGF2IGF2--In7In7D5FADD5FADDD--LoopLoopSW18SW18SW1943SW1943SW2588SW2588PRLRPRLR

BLADBLADDUMPDUMPCITRCITRCVMCVMXYXYGHGHmtDNAmtDNA

G6SG6SIGF2IGF2mtDNmtDNAA

HSP70HSP70PRLRPRLRPRLPRLESRESRmtDNAmtDNAZWZW

PRLPRLESRESRZWZWmtDNAmtDNA

GPIGPIPGDPGDXYXYIGF2IGF2mtDNAmtDNA

DNA barcodingDNA barcoding for pigsfor pigsHalHal--1843 gene for stress (AB), ESR gene for prolificacy 1843 gene for stress (AB), ESR gene for prolificacy (MN) and 16,613bp mtDNA for maternal origin, (MN) and 16,613bp mtDNA for maternal origin, especially on Despecially on D--loop region loop region ((1,175bp) of 29 SNPs and 1,175bp) of 29 SNPs and UTRn UTRn ((tacacgtgcgtacacgtgcg 10bp)10bp)17~27 17~27 in various breeds of pigs.in various breeds of pigs.

AB+MN+18AB+MN+18SNPSNP++UTRUTR+11+11SNPSNPmtDNA DmtDNA D--LoopLoop

A0021A0021--02 Lanyu02 Lanyu AAMNcaattgctcgtttctcag24tgctcgaaaccAAMNcaattgctcgtttctcag24tgctcgaaaccT0596T0596--01 Taoyuan01 Taoyuan AAMMcaattgctAAMMcaattgctttgtttgttttctccag24tgctcgaaaccag24tgctcgaaacttL0074L0074--10 Landrace 10 Landrace AAMNAAMNttgccatttgccattctcaccctcaccctccacaaa2727tgcttgcttataaaaaaattttY0150Y0150--03 Yorkshire03 Yorkshire AAMNcaattgcAAMNcaattgcctctgtttgttttctccagcag2525ttcccccccgaaacccgaaaccD0167D0167--05 Duroc05 Duroc AANNAANNttgccatttgccattctcaccctcaccctccacaaa2626tgcttgcttataaaaaaatctc

DNADNA

MMFFAARRMMFFAARR22--33--55--ZZ

PRLPRL--ESRESR--HSP70HSP70--PRLRPRLR

NNEEBBPPNNEEBBPP

MMMMEEEEAAAAPPPP CCGGTCCCCGGTCC

22--33--55--ZZ--mtDNAmtDNAPRLPRL--ESRESR--HSP70HSP70--PRLRPRLR--CoICoI

Native Chicken BarcodeNative Chicken Barcode

(Red feather)(Red feather)

(Black feather)(Black feather)

(Silky feather/Black meat)(Silky feather/Black meat)

BroilerBroiler

Chicken genom

e

CommonCommon--useuse•• DNA in food chainDNA in food chain•• Biogenetic banks in Biogenetic banks in

supporting scientific supporting scientific researchresearch

•• Genomic selection on Genomic selection on farm levelfarm level

•• WebWeb--based barcode based barcode system with food mileagesystem with food mileage

Acknowledgements •• Wu LabWu Lab– Mr. Yun-Yu Lai– Dr. Ren-Bao Liaw– Dr. Yuchia Huang– Dr. Ter-Yu Lin– Ms. Hsiu-Rong Tsai– Mr. Hsenn-Ben Chu

•• CollaboratorsCollaborators– Prof. Hsiu-Luan Chang– Dr. Lih-Ren Chen– Prof. Feng-Pang Cheng– Prof. Larry Schook, UIUC– Prof. Harris Lewin, UIUC– Dr. Le Thi Thuy, Vietnam– Prof. Rangsun Parnpai, Thailand– Dr. Libertado C. Cruz, Philippines– Prof. Chis Maxwell, Australia – Prof. Locksley E. McGann, Canada – Prof. Hoon Taek Lee, Korea

• Funding Sponsors–– COACOA–– TLRITLRI–– NAIFNAIF