CO-OP in EMD Serono - Merck Group€¦ · CO-OP in EMD Serono. 2 Title of Presentation | DD.MM.YYYY...
Transcript of CO-OP in EMD Serono - Merck Group€¦ · CO-OP in EMD Serono. 2 Title of Presentation | DD.MM.YYYY...
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Lande Fu
Jun 6, 2018
Enter the world of crystallography
CO-OP in EMD Serono
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2 Title of Presentation | DD.MM.YYYY
Before I start...
Try to find a question
I learnt the word crystallography for the first time when I was doing the interview.
Because I have no background on it, I don’t even have questions.
Just read the text book
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In the first few months...
Watch and Learn—wet lab experiment
Set up drops
Freeze crystals
Soak
Data collection
?
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Title of Presentation | DD.MM.YYYY4
I didn’t try to understand everything at once.
Instead I watch Ariele’s operation closely.
Look at other related experiments on the Internet.
In the first few months...
Watch and Learn—wet lab experiment
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5 Title of Presentation | DD.MM.YYYY
At the same time...
Watch and learn—CCP4 refinemnt
Learn CCP4
Learn COOT
Understand the protein structure
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6 Title of Presentation | DD.MM.YYYY
At the same time...
Watch and learn—CCP4 refinemnt
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Set up drops
Proteins grow in buffer
Soaking
Compound binds the proteins
Freeze cyrstals
Send to X-ray souce
Data colleciton
Get diffraction data
Refinement
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ARI
Gryphon
Make the plate automatically Take images of each drops in
schedule.
Scoring the drops
Rock
Imager
First project…
Optimization, setup the automation
Title of Presentation | DD.MM.YYYY8
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Second project…
Antibodies screening—find the right conditions
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10 Title of Presentation | DD.MM.YYYY
Extra project
Python script for PyMOL
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Extra project
Python script for PyMOL
Learn Python
Learn PymOL
Understand the Antibodies
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12 Title of Presentation | DD.MM.YYYY
Extra project
Python script for PyMOL