Chung, K Et Al - Structural And Molecular Interrogation of Intact Biological Systems_ Nature 2013

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    ARTICLEdoi:10.1038/nature12107

    Structural and molecular interrogation of

    intact biological systemsKwanghun Chung1,2, Jenelle Wallace1, Sung-Yon Kim1, Sandhiya Kalyanasundaram2, Aaron S. Andalman1,2,Thomas J. Davidson1,2, Julie J. Mirzabekov1, Kelly A. Zalocusky1,2, Joanna Mattis1, Aleksandra K. Denisin1, Sally Pak1,Hannah Bernstein1, Charu Ramakrishnan1, Logan Grosenick1, Viviana Gradinaru2 & Karl Deisseroth1,2,3,4

    Obtaining high-resolution information from a complex system, while maintaining the global perspective needed tounderstand system function, represents a key challenge in biology. Here we address this challenge with a method(termed CLARITY) for the transformation of intact tissue into a nanoporous hydrogel-hybridized form (crosslinked toa three-dimensional network of hydrophilic polymers) that is fully assembled but optically transparent andmacromolecule-permeable. Using mouse brains, we show intact-tissue imaging of long-range projections, local

    circuit wiring, cellular relationships, subcellular structures, protein complexes, nucleic acids and neurotransmitters.CLARITY also enables intact-tissue in situ hybridization, immunohistochemistry with multiple rounds of staining andde-staining in non-sectioned tissue, and antibody labelling throughout the intact adult mouse brain. Finally, we showthat CLARITY enables fine structural analysis of clinical samples, including non-sectioned human tissue from aneuropsychiatric-disease setting, establishing a path for the transmutation of human tissue into a stable, intact andaccessible form suitable for probing structural and molecular underpinnings of physiological function and disease.

    The extraction of detailed structural and molecular information fromintact biological systems has long been a fundamental challengeacross fields of investigation, and has spurred considerable technolo-gical innovation18. The study of brain structurefunction relation-ships in particular may benefit from intact-system tools912, and ingeneral, much valuable information on intra-system relationships

    and joint statistics will be accessible from full structural analysis ofintact systems rather than piecemeal reconstruction across prepara-tions. Yet even tissue structure in itself provides only a certain level ofinsight without detailed molecular phenotyping13,14, which is difficultto achieve within intact tissue.

    Current pioneering methods suitable for the mammalian braineither involve sectioning andreconstruction, or areincompatible withmolecular phenotyping, or both. Automated sectioning methods havebeen successfully used to map structure4,5,1518, in some cases withmolecular labelling. However, detailed reconstruction has typicallybeen limited in application to small volumes of tissue. On the otherhand, intact-imaging methods that extend the depth of light micro-scopy by reducing light scattering have emerged1921, but these pre-parations are incompatible with intact-tissue molecular phenotyping,

    and require many weeks of preparation to achieve partial tissue clear-ing. Studying intact systems with molecular resolution and globalscope remains an unmet goal in biology.

    We set ourselves the goal of rapidly transforming intact tissue intoan optically transparent and macromolecule-permeable constructwhile simultaneously preserving native molecular information andstructure. We took note of the fact that packed lipid bilayers areimplicated in rendering tissue poorly accessibleboth to molecularprobes and to photonsby creating diffusion-barrier properties rele-

    vant to chemical penetration, as well as light-scattering properties atthe lipidaqueous interface22,23. If lipid bilayers could be removed non-destructively, light and macromolecules might penetrate deep into thetissue, allowing three-dimensional imaging and immunohistological

    analysis without disassembly. But removing lipid membranes thatprovide structural integrity and retain biomolecules would inevitablydamage tissue with profound loss of cellular and molecular informa-tion. Therefore the provision of a physical framework would first berequiredto physically support the tissue and secure biologicalinforma-tion. We have developed and used such a technology, which we term

    CLARITY, that addresses these challenges.

    Hydrogel-electrophoretic tissue transmutationWe began by infusing hydrogel monomers (here, acrylamide andbisacrylamide), formaldehyde and thermally triggered initiators intotissue at4 uC (Fig. 1).In this step, formaldehyde notonly crosslinksthetissue, but also covalently links the hydrogel monomers to biomole-culesincluding proteins,nucleic acids and small molecules. Next,poly-merization of the biomolecule-conjugated monomers into a hydrogelmesh was thermally initiated by incubating infused tissue at 37 uC for3 h, at which point the tissue and hydrogel became a hybrid construct.Thishydrogeltissue hybridization physically supports tissue structureand chemically incorporates biomolecules into the hydrogel mesh.Importantly, lipids and biomolecules lacking functional groups for

    conjugation remain unbound and therefore can be removed fromthe hybrid. To extract lipids efficiently, we developed an ionic extrac-tion technique rather than using hydrophobic organic solubilization,for two mainreasons. First, althoughorganic solvents can extract lipidsor reduce refractive-index variations19,20,24, these solvents quenchfluorescence, thereby limiting imaging time. Light-sheet microscopyhas been used to image benzyl alcohol/benzyl benzoate(BABB)-treatedsamples while fluorescence persists19,20, but this approach is less com-patible with slower high-resolution imaging. Moreover, instability ofnative fluorescence in BABB constrains imaging of fine neuronal pro-

    jections or othermodest signals thatcan be easily quenched.Conversely,in the hydrogeltissue hybrid all fluorescent proteins tested, includ-ing green, yellow and red fluorescent proteins (GFP, YFP and RFP,

    1Department of Bioengineering, Stanford University, Stanford, California 94305, USA. 2CNC Program, Stanford University, Stanford, California 94305, USA. 3Department of Psychiatry and Behavioral

    Sciences, Stanford University, Stanford, California 94305, USA. 4Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA.

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    respectively), were robust to ionic detergent extraction (Fig. 2, Sup-plementary Fig. 1 and Supplementary Video 1). Second, whereas passivediffusion of detergent micelles would take many months to completelyextract lipids from the adult mouse brain, we developed an active-transport organ-electrophoresis approach, which we term electro-phoretic tissue clearing (ETC), that capitalizes on the highly chargednature of ionic micelles. This method expedites the extraction byordersof magnitude (Fig. 1 andSupplementaryFig. 2). We anticipated

    that the hydrogel would secure biomolecules and fine structural fea-tures such as membrane-localized proteins, synapses and spines inplace, whereas membranous lipids that cause light scattering and pre-

    vent penetration of macromolecules would be actively removed,leaving behind an otherwise fully assembled biological system suitablefor labelling and imaging intact.

    Whole adult mouse brain imagingTo test anticipated features of the technology, we processed withCLARITY (hereafter clarified) the brain of a 3-month-old line-Hmouse, in which the cytosolic fluorescent protein enhanced YFP(eYFP), under control of the Thy1 promoter sequence region, isexpressed in a subset of projection neurons25,26. Within 8 days, theintact adult brain was transmuted into a lipid-extracted and struc-

    turally stable hydrogeltissue hybrid. Scattering still occurs owing to

    heterogeneouslydistributed protein andnucleic acidcomplexes in thehybrid; however, after immersion in refractive-index-specified solu-tions matching the CLARITY hybrid (for example, 85% glycerol orFocusClear, both with a refractive index of,1.45), the intact brain(including heavily myelinated white matter, thalamus and brainstem)becomes uniformly transparent (Fig. 2ac and Supplementary Videos25). ETC is crucial: passive refractive-index matching of non-ETC-processed brain provided little clearing (Supplementary Fig. 3),whereas full CLARITY processing enabled imagingof the entire intactmouse brain at cellular resolution even using single-photon micro-scopy (Fig. 2df and Supplementary Videos 25); this is important asmany dyes and fluorescent proteins are better suited to single-photonrather than two-photon illumination27,28).

    Because imaging depth in clarified tissue appeared to be limitedonly by the working distance of the objective (here 3.6 mm, although

    8-mmworking distance/0.9 numerical aperture objectives are available),

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    presence of hydrogel monomers (blue), covalently linking tissue elements tomonomersthat arethenpolymerizedintoa hydrogel mesh (followedby a day-4wash step;Methods). Electric fields applied across the sample in ionicdetergentactively transport micelles into, and lipids out of, the tissue, leaving fine-structure and crosslinked biomolecules in place. The ETC chamber is depictedin the boxed region (Supplementary Fig. 2).

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    Figure 2 | Intact adult mouse brain imaging. Imaging was performed inadult mouse brains (3 months old). a, Cajal quote before CLARITY. b, Cajalquote after CLARITY: Thy1eYFP line-H mouse brain after hydrogeltissuehybridization, ETC and refractive-index matching (Methods). c, Fluorescenceimage of brain depicted in b. d, Dorsal aspect is imaged (single-photon (1p)microscopy), then brain is inverted and ventral aspect imaged. e, Three-dimensional rendering of clarified brain imaged (310 water-immersion

    objective; numerical aperture, 0.3; working distance, 3.6 mm). Left, dorsalhalf (stack size, 3,100 mm; step size, 20 mm). Right, ventral half (stack size,3,400mm; step size, 20 mm). Scale bar, 1 mm (Supplementary Videos 35).f, Non-sectioned mouse brain tissue showing cortex, hippocampus andthalamus (310 objective; stack size, 3,400mm; step size, 2 mm). Scale bar,400mm (Supplementary Video 2). gl, Optical sections from fshowingnegligible resolution loss even at ,3,400-mm deep: z5 446mm (g, h),z5 1,683mm (i, j) and z5 3,384mm (k, l). h, j and l, boxed regions ing, i and k, respectively.Scale bars,100 mm. m, Cross-section of axonsin clarifiedThy1channelrhodopsin2 (ChR2)eYFP striatum: membrane-localizedChR2eYFP (1-mm-thick coronal block;363 glycerol-immersion objective;numerical aperture, 1.3; working distance, 280mm). Scale bar, 5mm.n, Dendrites and spines of neurons in clarified Thy1eYFP line-H cortex(1-mm-thick coronal block; 363 glycerol objective). Scale bar, 5 mm.

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    to image the 56-mm-thick adult mouse brain, we next imaged thedorsal half ofthe brainfollowed by inversionand imaging ofthe ventralhalf (Fig. 2d, e). Figure 2f shows a volume of unsectioned mouse brainwith visualization through the cortex, hippocampus and thalamus(Fig. 2gl and Supplementary Video 2). We observed that tissueexpanded with ETC and returned to its original size after refractive-index matching; thistransient change did not cause net tissue deforma-tion, andremaining secured in place were fine structural details such as

    membrane-localized proteins (Fig. 2m), dendritic spines (Fig. 2n) andsynaptic puncta(Fig. 3eg).We found that clarifiedtissue may be com-patible withdownstreamelectron microscopy,retaining key ultrastruc-tural features such as synapses and postsynaptic densities, althoughwith current methods not all relevant ultrastructures are apparent,probably owing to lack of membrane lipids (Supplementary Fig. 4).CLARITY thus allows rapid high-resolution optical access to denseintact tissue and, if needed, subsequent electron microscopy analysis.We next sought to determine whether this design enabled intact-tissuemolecular phenotyping.

    Molecular phenotyping of intact-tissue volumesInterrogating molecular features at subcellular resolution in an intactbrain with known global wiring properties may be of value; however,

    conventional labelling techniques involve: (1) loss of native moleculesafter the permeabilization required for access to intracellular targets29;(2) time-intensive thin sectioning and reconstruction5; or (3) damagewhen multiple rounds of labelling are attempted due to harsh probe-removal processes5,3032. We anticipated that CLARITY could allowintact-tissue and multiple-round molecular phenotyping by over-coming these three main difficulties. First, CLARITY may preservenative antigens with unusual completeness owing to the hydrogel-hybridization process. To quantify molecular preservation associatedwithtissuehydrogelfusion, we comparedprotein lossin clarified mousebrain to loss from conventional methods (Fig. 3a). Of total protein,,65% was solubilized (lost) when conventionally paraformaldehyde(PFA)-fixed tissue blocks were cleared by 4% SDS for 1 week. Scale, atissue-clearing method using 4 M urea, allowed ,41% protein lossover the same interval. Even PFA-fixed tissue treated only with 0.1%Triton X-100 in PBS buffer, a mild detergent-based permeabilizationsolution used in conventional histology, allowed,24%loss of protein.However, when hydrogel-hybridized tissue was cleared with the strin-gent 4% SDS solution of CLARITY, only,8% protein loss was seen,indicating that chemical tethering of biomolecules into hydrogel meshcan enhance the preservation of molecular tissue components.

    Second, we found that CLARITY, which increases tissue permeab-ility by replacing lipid bilayers with nanoporous hydrogel, enabledrapid diffusion of molecular probes deep into intact tissue, and there-fore allows access to preserved biomolecules without sectioning. In a1-mm-thickclarified coronal block of mouse brain, uniformly antibodystained over 3 days (Fig. 3b and Supplementary Video 6), quantitativeco-localization analysis revealed that eYFP fluorescence and anti-GFPstaining overlapped throughout the block (Fig. 3c, d). We next wantedto know whether whole-mount mouse brain immunolabelling waspossible; although the lipid-cleared brain is highly permeable, it waspossible that antibodies would notdiffuse throughoutthetissue on prac-tical timescales. We conducted immunohistochemistry for tyrosinehydroxylase in clarified intact mouse brain, and found that 2 weeksof incubation eachfor primaryand secondary antibody enabled2.5 mmof penetration from each surface, enough to stain,5-mm-thick adultmouse brains (Supplementary Fig. 5 and Supplementary Video 7).Tyrosine-hydroxylase-positive cell bodies and projections werereadily

    visualized throughout the brain, and only at the deepest point wasmoderate signal attenuation seen, indicating that antibody penetra-tion was not quite maximized and may be further enhanced by tuningconditions.

    To investigate axonal projections of the tyrosine hydroxylase neu-

    rons further, we clarified, tyrosine-hydroxylase-stained and imaged

    1-mm-thick coronal blocks of mouse brain using a high numericalaperture objective (0.95). As shown in Fig. 3j, k, Supplementary Figs68 and Supplementary Video 8, projections of tyrosine-hydroxylase-

    positive fibres were readily visualized in the neocortex, nucleus

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    Figure 3 | Molecular phenotyping in intact tissue. a, Protein loss in clarifiedmouse brain compared to conventional methods (see SupplementaryInformation for more details); error bars denote s.e.m.; n5 4 for eachcondition. b, Renderingof a 1-mm-thicknon-sectioned coronal blockof Thy1eYFP mouse brain immunostained for GFP. The tissue was ETC-cleared(1 day), immunostained (3 days) andimaged (310 water-immersion objective;single-photon excitation). Left, eYFP (green); middle, anti-GFP (red); right,overlay. Scale bar, 500 mm (Supplementary Video 6). c, Three-dimensionalrendering of the boxed region in the cortex in b shows eYFP fluorescence (left)and anti-GFP staining (right). d, Left, co-localization: Manders overlapcoefficient plotted versus depth43. Right, optical sections at different depths inthree-dimensional rendering. Scale bar, 100mm. e, f, 500-mm-thick block ofline-H mouse brain (2 months old) clarified for 1 day and immunostained forsynapsin I (red) and PSD-95 (blue) for 3 days (Methods) (363 glycerolobjective; single-photon excitation). e, Left, optical sections (z5 20mm, z5200mm). Right, enlarged images of boxed regions on left. Individual synapticpuncta resolved throughout depth. White depicts eYFP staining. f, Averageimmunofluorescence cross-section of PSD-95 puncta at z5 20mm (top) andz5 200mm (bottom). g, Full width at half maximum (FWHM) of averageimmunofluorescence cross-section of PSD-95 puncta versus depth. Insets,average puncta at z5 20mm and z5 200mm. h, Hippocampal staining. Left,GABA; middle, parvalbumin (PV); right, overlay. 500-mm-thick block of wild-type mouse brain (3 months) clarified (1 day) and immunostained (3 days)

    (3

    25 water-immersion objective; numerical aperture, 0.95; workingdistance, 2.5 mm; single-photon excitation). Scale bar, 20mm. i, in situhybridization. Clarified 500-mm mouse brain block showing dopaminereceptor D2 (Drd2) mRNA in the striatum. LV, lateral ventricle. Blue, DAPI.50-base-pair RNA probes for Drd2 visualized with FastRed (325 water-immersion objective; single-photon excitation (555 nm) for FastRed, two-photon excitation (720 nm) for DAPI). Scale bars: left, 100mm; right, 20 mm.

    j, k, Axonalfibres of tyrosine hydroxylase (TH)-positive neurons in the nucleusaccumbens (NAc) and caudateputamen (CPu). j, Three-dimensionalrendering of 1-mm-thick clarified mouse brain block stained for tyrosinehydroxylase (red) and DAPI (green). aca, anterior commissure. Scale bar,500mm. k, Maximum projection, NAc/aca volume in j. Scale bar, 50 mm.

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    accumbens, caudateputamen and amygdala. Interestingly, certaincells in the infralimbic cortex and amygdala appeared much moreheavily invested by tyrosine hydroxylase fibres than neighbouring cells(Supplementary Figs 6f and 8 and Supplementary Video 8). Furthersupporting specificity of staining in clarified tissue (Fig. 3j, k andSupplementary Fig. 7), visualized tyrosine hydroxylase projectionsspared anterior commissure while heavily investing the surroundingnucleus accumbens and caudateputamen as expected.

    We next considered that CLARITY could potentially enable ana-lysis of subcellular molecular architecture in large volumes with reso-lution at the diffraction limit of light microscopy33, in an approachcomplementary to thin mechanical sectioning and three-dimensionalreconstruction5,34,35. Resolving submicron-scale structures deep intissue poses a challenge as the high numerical aperture objectivesusedfor high-resolution imaging are susceptible to optical aberrationcaused by refractive-index variations in tissue, which blurs imagesand impairs differentiation of adjacent fluorescent signals, such aspre- and postsynaptic puncta. But when a clarified 500-mm-thickblock of Thy1eYFP mouse brain was immunolabelled for synapsinI (presynaptic) and PSD-95 (postsynaptic) and imaged with a 363glycerol-immersion objective (numerical aperture, 1.3; working dis-tance, 280 mm), throughout the tissue volume we could unequivocally

    identify individual paired pre- and postsynaptic puncta, molecu-larly defining identity and position of putative excitatory synapses(Fig. 3eg). The full-width at half-maximum of the point-spreadfunction was uniform throughout the block, indicating that loss ofresolution is negligible even near the diffraction limit of conventionallight microscopy (Fig. 3f, g and Supplementary Figs 9 and 10) anddemonstrating another feature of the technique: removal of lipidmembranes ensures that tissue refractive index remains nearly con-stant throughout large volumes, allowing high-resolution imaging.We also found that the CLARITY hydrogel-conjugation processpreserves small molecules such as the neurotransmitter GABA (c-aminobutyric acid, Fig. 3h) and (for in situ hybridization) messengerRNAs (Fig. 3i), paving theway formultimodal combinatorial labellingwithin unsectioned tissue.

    Finally, CLARITY was found to enable multi-round molecularphenotyping; the stable framework allowed effective removal ofantibodies without fine structural damage or degraded antigenicity.Traditional elution requires incubation in acidic buffer with potas-sium permanganate (KMnO4) oxidation or heat, harsh treatmentsthat cause antigen loss and fluorescence quenching5,3032. But in thehydrogel context of increased structural/chemical stability, the ionicdetergent (4% SDS/neutral-pH buffer) used initially to create clarifiedtissue can be re-used to denature antibodies and disrupt binding,and the permeability of clarified tissue can facilitate rapid antibodyexchange. We performed three consecutive rounds of staining in1-mm-thick coronal blocks from a Thy1eYFP H-line mouse brain(Fig.4 and Supplementary Videos 1014), observingeffectiveantibodyremoval and preserved eYFP-positive neuronal morphology as wellas re-staining capability. Although further quantification would berequired to fully map the extent to which CLARITY secures molecularinformation, this result shows that elution in clarified tissue largelypreserves integrity of tissue structure, cellular architecture, fluores-cence signals (Fig. 4df) and 49,6-diamidino-2-phenylindole (DAPI)DNA staining (Fig. 4g). Moreover, repeated tyrosine hydroxylasestaining in the first and third rounds maintained signal pattern andintensity, confirming that antigenicity is retained throughout multiplerounds of staining and elution.

    Human tissue imaging and molecular phenotypingCLARITY is, in principle, applicable to any tissue: adult zebrafishimaging and phenotyping are demonstrated36 in SupplementaryFig. 12, and we also tested post-mortem human brains. With impor-tant and rare tissue from clinical brain banks residing in formalin, one

    concern is that these maximally crosslinked samples could be poorly

    suited to CLARITY, and certainly the mapping of connectivity in thehuman brain (ref. 37 and http://www.neuroscienceblueprint.nih.gov/connectome/) at cellular resolution has been hampered by lack of

    methods for labelling individual neurites over distances, as the injec-tion of dyes into living brain or in vivo genetic labelling are feasibleonly in animals38,39. If extensively fixed human tissues were suitablefor CLARITY, new avenues would be opened for the study of clinicalsamples.

    We found that CLARITY wassuitablefor such long-banked humanbrain, allowing immunohistological visualization and identificationof neurons and projections over large volumes (Fig. 5ag and Sup-plementary Videos 1517). In 0.5-mm-thick blocks of frontal lobefrom an autistic patient, stored in formalin for .6 years, we wereable to stain for axons with neurofilament protein and myelin basicprotein, and trace individual fibres (Fig. 5e and Supplementary Video15). In addition, by staining for parvalbumin it was possible to visua-lize the distribution of parvalbumin-positive neurons in the neo-

    cortex over large volumes (6.73 4.73 0.5 mm), and trace individual

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    Figure 4 | Multi-round molecular phenotyping of intact tissue. a, Firstround. Rendering of 1-mm-thick Thy1eYFP block immunostained fortyrosine hydroxylase in non-sectioned form. ETC-cleared (1 day) andimmunostained (6 days). Scale bar, 500 mm (Supplementary Video 10).b, Antibodies eluted from block in a(4% SDS, 60 uC for 0.5 days). Tyrosinehydroxylase signal was removed and eYFP fluorescence retained(Supplementary Video 11). c, Second round. Three-dimensional rendering ofsame block now immunostained for parvalbumin (red), glial fribrillary acidicprotein (GFAP) (blue) and DAPI (white) (Supplementary Video 12).

    df, Maximum projections of 100 mm volume of yellow-boxed regions ina, b and c, respectively.eYFP-positiveneuronspreserved. cp, cerebral peduncle;SNR, substantia nigra. Scalebar, 100mm. g, Optical section of white/dotted-boxregion in c showing DAPI. CA, cornu ammonis; DG, dentate gyrus. Scale bar,100mm. h, i, Tyrosine hydroxylase channel of white box regions in a(h) and

    j (i). Tyrosine hydroxylase antigenicity preserved through multiple elutions.Scale bar, 100mm. j, Third round. Block in ac immunostained for tyrosinehydroxylase (red)and choline acetyltransferase (ChAT)(blue) (SupplementaryVideo 13). k, Three-dimensional view of hippocampus in c showing eYFP-expressing neurons (green), parvalbumin-positive neurons (red) and GFAP(blue). Alv, alveus. Scale bar, 200 mm (Supplementary Video 14).

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    parvalbumin-labelled processes (Fig. 5gn and Supplementary Videos16 and 17).

    Unlike mechanical sectioning methods that may involve defor-mation of tissue and uncertainty in registration across sections,CLARITY preserves continuity of structure, which not only allows

    tracing of neurites over distances, but also provides a class of distinct

    information about three-dimensional and topological morphology oftraced neurons. As one example, we found that many parvalbumin-positive interneurons in thishuman sample, particularlyin deep layers,showed isoneuronal and heteroneuronal dendritic bridges (Fig. 5gm,Supplementary Fig. 13, Supplementary Video 17 and SupplementaryTable 2). These ladder-like connections, not typical of age- and sex-matched normal brain (Supplementary Fig. 14), instead resembleabnormalities observed with mutations in the Down syndrome cell-

    adhesion molecule (Dscam) protein40

    or protocadherins41

    ; mutationsin the latter family are associated with autism-spectrum disorder42.Although extensive work would be required to define the incidenceand implications of abnormal parvalbumin neurons, the observationillustrates the structural and molecular capability that clarified tissueprovides by virtue of volumetric continuity, which may help to shedlight on structural underpinnings of neuropsychiatric disease.

    DiscussionWith this hydrogeltissue fusion and electrophoretic-clearing tech-nology, intact tissue can be rapidly transformed into an optically andchemically accessible form while retaining structural and molecularinformation, thereby enabling the imaging of entire adult vertebratebrains as well as multiple-round molecular phenotyping without thin

    sectioning. Currently, the non-corrected water-immersion objectivesused, rather than clarified tissue properties, represent the main limit-ing factor for maximizing imaging depth and resolution; specializedadaptive optics or CLARITY-optimized long-working-distance objec-tives will further enhance the acquisition of integrated structural andmolecular information from intact systems.

    Turningimmense data setsinto useful insights remainsa keychallenge.Computational approaches to image segmentation, three-dimensionalregistration and automated tracing require further development.Moreover, for the subset of applications relevant to circuit connec-tivity,CLARITYdoes not in itself supplant electron microscopy. How-ever, the potential electron microscopy compatibility of CLARITY(Supplementary Fig. 4) may offer a two-step approach in probingconnectivitywith synaptic resolution; CLARITY withlight microscopymay first be used to visualize and trace fluorescently labelled neuronsand projections in the whole brain, followed by electron microscopyanalysis of small volumes to define the patterns andrules (for example,postsynaptic cell target type) followed by axon terminals and synapticcontacts. Notably, owing to the absence of lipids, osmium tetroxide/uranyl acetate staining alone with electron microscopy does not pro-

    vide enough contrast to identify all ultrastructural boundaries, andadditional methodological tissue-preparation work is indicated.

    Although much remains to be refined, newly enabled access tointact-system properties maydovetailwith existingoptogenetic-controlor activity-imaging methods. For example, cells studied in local andglobal structural detail with CLARITY could be the same cells previ-ously visualized for activity, or controlled to modulate performance,during behavioural assays in the same organism with proper registra-tion. Moreover, efficient molecular phenotyping applied to immediate

    early gene products may help to map populations with altered activitydownstream of directly modulated populations, across the same intactbrain and in the context of global wiring maps. Together with its capa-bilityfor intersectionaldefinition through multiplerounds of labelling,and broadapplicationdomain (includingmouse, zebrafish and human),these data suggest that CLARITY provides access to structural andmolecular information that may help to support integrative under-standing of large-scale intact biological systems.

    METHODS SUMMARYCLARITY. CLARITY refers to transformation of intact biological tissue into ahybrid form in which specific components are replaced with exogenous elementsthat provide new accessibility or functionality. Initially, the term was an acronymto describe the Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel. However,

    CLARITY is more inclusively defined because infused elements need not be

    PV

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    Figure 5 | Human brain structural/molecular phenotyping. Human BA10500-mm-thick intact blocks clarified (1 day) and immunostained (3 days) (325water-immersion objective). a, Optical section: myelin basicprotein(MBP) andparvalbumin staining. White arrowheads indicate membrane-localized myelinbasic protein around parvalbumin-positive projections. Scale bar, 10 mm.b, Tyrosine hydroxylase and parvalbumin staining (maximum projection;

    120m

    m volume; step size, 0.5m

    m). Scale bar, 50m

    m. c, Optical section:neurofilament (NP) and GFAP. Scale bar, 20 mm. d, Somatostatin andparvalbumin staining (maximum projection; 63mm volume; step size, 0.5 mm).Scale bar, 20mm. e, Rendering of neurofilament-positive axonal fibres. Red,traced axon across volume. Scale bar, 500 mm. Inset: boxed region. Scale bar,20mm (Supplementary Video 15). f, Visualization of parvalbumin-positiveneurons in theneocortexof autismcase;layers identified as describedin ref. 44.Scale bar, 500mm (Supplementary Video 16). g, Yellow-boxed region infshowing parvalbumin-positive cell bodiesand fibresin layers4, 5 and6. Threerepresentative parvalbumin-positive interneurons in layer 6 with ladder-shaped hetero- or iso-neuronal connections were traced (green, purple, blue).Scale bar, 100mm (Supplementary Video 17). h, Three-dimensional renderingof abnormal neurons in g; yellow arrowheads (1, 2) indicate ladder-shapedstructures shown below in i and k. Scale bar, 80mm. i, Zoomed-in maximumprojection of 8 mm volume showing morphology of ladder-shaped structureformed by neurites from a single neuron. Scale bar, 10 mm. j, Tracing of

    structure in i. k, Maximum projection of 18mm volume showing ladder-shapedstructure formed by neurites from two different neurons. Scale bar, 10 mm.l, Tracing of structure in k. m, Iso- and hetero-neuronal dendritic bridges perneuron. Neurons selected randomly and traced in software (Methods);dendritic bridges were manually counted. **P, 0.05; error bars denote s.e.m.n5 6 neurons for both superficial and deep layers of autism case and n5 4neurons for both superficial and deep layers of control case. n, Three-dimensional reconstruction of a neuron in layer 2 (superficial) of the autismcase. Typical avoidance of iso-dendritic contact was observed.

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    exclusively hydrogel monomers or acrylamide-based, and the properties ofinfused elements may be adjusted for varying degrees of clarity, rigidity, mac-romolecule-permeability or other functionality.CLARITY for mouse brain. Adult mice (412 weeks old) were anaesthetizedwith Beuthenasia-D and perfused transcardially with a mixture of 4% (wt) PFA,4% (wt/vol) acrylamide, 0.05% (wt/vol) bis-acrylamide, 0.25% (wt/vol) VA044and PBS. Brains were then extracted and incubated in the same solution at 4 uCfor 3 days. Solutiontemperaturewas thenincreased to 37 uC to initiatepolymeriza-tion. After 3 h at 37 uC, hydrogel-embedded brains were placed in a custom-built

    organ-electrophoresis chamber. While sodium borate buffer (200mM, pH 8.5)containing 4% (wt/vol) SDS was circulated through the chamber, 1060V wasapplied across the brains at 3750 uC for 2 days. After clearing, brains were incu-bated in PBS at 37 uC for 2 days to remove SDS. To prepare 1-mm-thick coronalblocks of mouse brain for immunostaining, hydrogel-embedded and clarifiedbrains were cut into 1-mm-thick blocks using a mouse brain matrix (Ted Pella).Blocks were then cleared by electrophoresis for 1 day as described above.CLARITY for human brain. Frontal lobe (BA10) from post-mortem humanbrain tissue was sliced into 500-mm-thick blocks using vibratome. Blocks wereincubated in 4% (wt) PFA, 4% (wt/vol) acrylamide, 0.25% (wt/vol) VA044 andPBS at 4 uC for 1 week. The temperature of the solution was then increased to37 uC toinitiatepolymerization.After 3 h incubation at 37uC, hydrogel-embeddedtissues were placed in the custom-built electrophoresis chamber. While sodiumboratebuffer(200 mM,pH 8.5)containing4% (wt/vol) SDS circulatedthrough thechamber, 1060 V was applied at 3750uC for 1 day. Tissues were then incubatedin PBS at 37 uC for 1 day to remove SDS.

    Full Methods and any associated references are available in the online version ofthe paper.

    Received 1 September 2012; accepted 20 March 2013.

    Published online 10 April 2013.

    1. Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescencemicroscopy. Science 248, 7376 (1990).

    2. Carmeliet, P. & Tessier-Lavigne, M. Common mechanisms of nerve and bloodvessel wiring. Nature 436, 193200 (2005).

    3. Helmchen, F. & Denk, W. Deep tissue two-photonmicroscopy. Nature Methods 2,932940 (2005).

    4. Denk, W. & Horstmann, H. Serial block-face scanning electron microscopy toreconstruct three-dimensional tissue nanostructure. PLoS Biol. 2, e329 (2004).

    5. Micheva,K.D. & Smith,S. J.Arraytomography:a newtoolforimagingthemoleculararchitecture and ultrastructure of neural circuits. Neuron 55, 2536 (2007).

    6. Livet,J. et al. Transgenic strategies for combinatorial expression of fluorescent

    proteins in the nervous system. Nature 450, 5662 (2007).7. L i, A . etal. Micro-optical sectioning tomography to obtain a high-resolutionatlas of

    the mouse brain. Science 330, 14041408 (2010).8. Botcherby, E. J. et al. Aberration-free three-dimensional multiphoton imaging of

    neuronal activity at kHz rates. Proc. Natl Acad. Sci. USA 109, 29192924 (2012).9. DeFelipe,J. From the connectome to the synaptome: an epic love story. Science

    330, 11981201 (2010).10. Petersen, C. C. H. The functional organization of the barrel cortex. Neuron 56,

    339355 (2007).11. Kasthuri, N. & Lichtman, J. W. The rise of the projectome. Nature Methods 4,

    307308 (2007).12. Lichtman,J. W.,Livet,J. & Sanes,J. R. A technicolour approachto theconnectome.

    Nature Rev. Neurosci. 9, 417422 (2008).13. Mombaerts,P. etal. Visualizingan olfactory sensory map. Cell 87, 675686 (1996).14. Insel, T. R. & Young,L. J. The neurobiology of attachment. Nature Rev. Neurosci. 2,

    129136 (2001).15. Micheva, K. D., Busse, B., Weiler, N. C., ORourke, N. & Smith, S. J. Single-synapse

    analysis of a diverse synapse population: proteomic imaging methods andmarkers. Neuron 68, 639653 (2010).

    16. Bock,D. D. etal. Networkanatomyand invivo physiologyof visualcortical neurons.Nature 471, 177182 (2011).

    17. Briggman, K. L., Helmstaedter, M. & Denk, W. Wiring specificity in the direction-selectivity circuit of the retina. Nature 471, 183188 (2011).

    18. Ragan, T. et al. Serial two-photon tomography for automated ex vivo mousebrainimaging. Nature Methods 9, 255258 (2012).

    19. Dodt, H. U., Leischner, U. & Schierloh, A. Ultramicroscopy: three-dimensionalvisualization of neuronal networks in the whole mouse brain. Nature Methods 4,331336 (2007).

    20. Erturk, A., Mauch, C., Hellal, F. & Forstner, F. Three-dimensional imaging of theunsectioned adult spinal cordto assess axon regeneration and glial responsesafter injury. Nature Med. 18, 166171 (2012).

    21. Hama, H. et al. Scale: a chemical approach for fluorescence imaging andreconstruction of transparentmouse brain.NatureNeurosci. 14,14811488 (2011).

    22. Cheong, W., Prahl, S. & Welch, A. A review of the optical properties of biologicaltissues. IEEE J. Quant. Electron. 26, 21662185 (1990).

    23. Sykova, E. & Nicholson, C. Diffusion in brain extracellular space. Physiol Rev. 88,12771340 (2008).

    24. Becker, K., Jahrling, N., Saghafi, S., Weiler, R. & Dodt, H.-U. Chemical clearing anddehydration of GFP expressing mouse brains. PLoS ONE7, e33916 (2012).

    25. Porrero, C., Rubio-Garrido, P., Avendano, C. & Clasca, F. Mapping of fluorescentprotein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice. Brain Res. 1345, 5972 (2010).

    26. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiplespectral variants of GFP. Neuron 28, 4151 (2000).

    27. Shaner, N. C., Steinbach, P. A. & Tsien, R. Y. A guide to choosing fluorescentproteins. Nature Methods 2, 905909 (2005).

    28. Drobizhev, M., Makarov, N. S., Tillo, S. E., Hughes, T. E. & Rebane, A. Two-photonabsorptionpropertieso f fluorescent proteins.NatureMethods 8, 393399 (2011).

    29. Goldenthal, K. L., Hedman, K., Chen, J. W.,August, J. T. & Willingham,M. C.Postfixation detergent treatment for immunofluorescences uppresses localizationof some integral membrane proteins.J. Histochem.Cytochem. 33,813820 (1985).

    30. Tramu, G. An efficient method of antibody elution for the successive orsimultaneous localizationof two antigensby immunocytochemistry.J. Histochem.Cytochem. 26, 322324 (1978).

    31. Wahlby, C., Erlandsson, F., Begntsson, E. & Zetterberg, A. Sequentialimmunofluorescence staining and imageanalysis for detectionof largenumbersof antigens in individual cell nuclei. Cytometry71, 3241 (2002).

    32. Kolodziejczyk, E. & Baertschi, A. J. Multiple immunolabeling in histology: a newmethod using thermo-inactivation of i mmunoglobulins.J. Histochem. Cytochem.34, 17251729 (1986).

    33. Zhou, Q., Homma, K. J. & Poo, M. Shrinkage of dendritic spines associated withlong-term depression of hippocampal synapses. Neuron 44, 749757 (2004).

    34. Harlow, M. L., Ress, D., Stoschek, A., Marshall, R. M. & McMahan, U. J. Thearchitecture of active zone material at the frogs neuromuscular junction. Nature409, 479484 (2001).

    35. Chen, X. et al. Organizationof the corestructureof the postsynapticdensity. Proc.

    Natl Acad. Sci. USA 105, 44534458 (2008).36. Macdonald, R. Zebrafish immunohistochemistry. Methods Mol. Biol. 127, 7788

    (1999).37. Sporns,O., Tononi,G. & Kotter,R. Thehuman connectome: a structural description

    of the human brain. PLOS Comput. Biol. 1, e42 (2005).38. Wickersham, I. R.,Finke,S., Conzelmann,K.-K.& Callaway,E. M. Retrogradeneuronal

    tracing with a deletion-mutant rabies virus. Nature Methods 4, 4749 (2007).39. Miyamichi, K. et al. Cortical representations of olfactory input by trans-synaptic

    tracing. Nature 472, 191196 (2011).40. Matthews, B. J. et al. Dendrite self-avoidance is controlled by Dscam. Cell 129,

    593604 (2007).41. Lefebvre, J. L.,Kostadinov,D., Chen, W. V.,Maniatis,T. & Sanes,J. R. Protocadherins

    mediate dendritic self-avoidance in the mammaliannervous system. Nature 488,517521 (2012).

    42. Morrow, E. M. et al. Identifying autism loci and genes by tracing recent sharedancestry. Science 321, 218223 (2008).

    43. Dunn, K. W., Kamocka, M. M. & McDonald, J. H. A practical guide to evaluatingcolocalization in biological microscopy. Am. J. Physiol. Cell Physiol. 300,

    C723C742 (2011).44. Conde, F.,Lund,J. S.,Jacobowitz, D.M., Baimbridge,K. G.& Lewis,D. A.Local circuitneurons immunoreactive for calretinin, calbindin D-28k or parvalbumin inmonkey prefrontal cortex: distribution and morphology.J. Comp. Neurol. 341,95116 (1994).

    Supplementary Information is available in the online version of the paper.

    Acknowledgements This work was funded by a National Institutes of Health (NIH)DirectorsTransformativeResearchAward(TR01) to K.D.fromNIMH,as wellas byNSF,the Simons Foundation, and the President and Provost of Stanford University. K.D. isalso funded by NIDA, the DARPA REPAIR program, and the Wiegers, Snyder, Reeves,Gatsby, and Yu Foundations. K.C. is supported by the Burroughs Wellcome FundCareerAwardat theScientific Interface.S.-Y.K. is supported bya SamsungScholarship,A.S.A. by the Helen Hay Whitney Foundation, K.A.Z. and A.K.D. by an NSF GraduateResearch Fellowship and J.M. by the NIH MSTP. We acknowledge H. Vogel, L. Luo,L. Schwarz, M. Monje, S. Hestrin and D. Castaneda for advice, and the Autism TissueProgramfor providing human brain tissue,as wellas J. J. Perrino,J. Mulholland andtheCellSciences Imaging Facilityat Stanford forelectron microscopy imaging andadvice.

    We would also like to thank the entire Deisseroth laboratory for discussions andsupport. CLARITY resources and protocols are freely supported online (http://CLARITYresourcecenter.org).

    Author Contributions K.C. and K.D. conceived and designed the experiments andwrote the paper. K.C. led development of the CLARITY technology and itsimplementation.K.C. andS.K. clarified samples.K.C. imaged samples.K.C.,S.-Y.K., S.K.,J.W., K.A.Z., S.P., J.J.M., J.M., V.G. and H.B. prepared animals. J.W. performed tracing.T.J.D. and A.S.A. performed image processing. K.C., J.W., J.J.M. and A.K.D. wrote theCLARITY protocol. A.K.D. created AutoCAD drawings. K.C. and L.G. createdSupplementary Videos. A.S.A. contributed to in situ and zebrafish data. C.R., L.G. andV.G. contributed to set-up of the relevant laboratory infrastructure. K.D. supervised allaspects of the work.

    Author Information Reprints and permissions information is available atwww.nature.com/reprints.The authors declare competing financial interests: detailsare available in the online version of the paper. Readers are welcome to comment onthe online version of the paper. Correspondence and requests for materials should beaddressed to K.D. ([email protected]) .

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    http://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/doifinder/10.1038/nature12107http://clarityresourcecenter.org/http://clarityresourcecenter.org/http://www.nature.com/reprints.Thehttp://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/doifinder/10.1038/nature12107mailto:[email protected]:[email protected]://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/reprints.Thehttp://clarityresourcecenter.org/http://clarityresourcecenter.org/http://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/doifinder/10.1038/nature12107http://www.nature.com/doifinder/10.1038/nature12107
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    METHODSCLARITY. CLARITY refers to transformation of intact biological tissue into ahybrid form in which specific components are replaced with exogenous elementsthat provide new accessibilityor functionality. Initially, the term was an acronymto describe the Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel. However,CLARITY is more inclusively defined because infused elements need not beexclusively hydrogel monomers or acrylamide-based, and the properties ofinfused elements may be adjusted for varying degrees of clarity, rigidity, mac-

    romolecule-permeability or other functionality.Immunostaining of CLARITY-processed mouse braintissue.For GFP stainingshown in Fig. 3bd, the clarified 1-mm-thick block of Thy1eYFP line-H mouse(2 months old) brain was incubated at 37 uC for 2 days in 0.1% Triton X-100 (wt/

    vol), anti-GFP antibody conjugated with Alexa Fluor 594 (Invitrogen, 1:50 dilu-tion), 1 M sodium borate buffer solution, pH 8.5, followed by wash at 37 uC for1 day with 0.1% (wt/vol) Triton X-100 and 1 M sodium borate buffer, pH 8.5. Fortyrosine hydroxylase staining of whole mouse brain in Supplementary Fig. 4, theclarifiedC57BL/6mouse(4 weeksold) brain wasincubatedat 37 uCfor2weeksin0.1% (wt/vol) Triton X-100, primary antibody (dilution, 1:50), 0.5 M sodiumborate buffer solution, pH 8.5, followed by wash at 37 uC for 1 week with 0.1%(wt/vol) Triton X-100 and 0.5 M sodium borate buffer, pH 8.5. The tissue wasthen incubated at 37 uC for 2 weeks in 0.1% (wt/vol) Triton X-100, secondaryantibody (dilution, 1:50) and 0.5 M sodium borate buffer solution, pH 8.5, fol-lowed by wash at 37 uC for 1 week with 0.1% (wt/vol) Triton X-100 and 0.5 M

    sodium borate buffer, pH 8.5. For other staining, the clarified 1-mm-thick blockof Thy1eYFP H-line mouse (2 months old) brain was incubated at 37 uC for2 days in 0.1% (wt/vol) Triton X-100, primary antibody (dilution, 1:501:100)and 1 M sodium borate buffer solution, pH 8.5, followed by wash at 37 uC for1 daywith 0.1% (wt/vol) TritonX-100 and1 M sodiumboratebuffer, pH 8.5. Thetissue was then incubated at 37 uC for 1 day in 0.1% (wt/vol) Triton X-100,secondary antibody (dilution, 1:50 1:100) and 1 M sodium borate buffer solu-tion, pH 8.5, followed by wash at 37uC for 1 day with 0.1% (wt/vol) Triton X-100and 1 M sodium borate buffer, pH 8.5.

    Immunostaining of CLARITY-processed post-mortem human brain tissue.Clarified 500-mm-thick blocks were incubated at 37 uC for 1 day in 0.1% (wt/vol)Triton X-100, primary antibody (dilution, 1:501:100) and 1 M sodium boratebuffer solution, pH 8.5, followed by wash at 37 uC for 12 h with 0.1% (wt/vol)Triton X-100 and 1 M sodium borate buffer, pH 8.5. The tissue was then incu-bated at 37 uC for 1 day in 0.1% (wt/vol) Triton X-100, secondary antibody(dilution, 1:501:100) and 1 M sodium borate buffer solution, pH 8.5, followed

    bywashat37uC for 12h with0.1%(wt/vol)TritonX-100 and 1 M sodiumboratebuffer, pH 8.5.

    Imaging of CLARITY-processed mouse brain tissue. For imaging the intactThy1eYFP H-line mouse brain (Fig. 2e), the clarified brain (3 months old) wasincubatedin FocusClear, a water-based immersionmedium, for2 days. Thebrainwasthen enclosed between twocoverglass-bottom Petri dishes. Thedorsalhalf ofthe brain was first imaged (stack size, 3.1 mm; step size, 20mm) using a Leica SP5system equipped with the 310 water-immersion objective (Leica HCX AOP L;numerical aperture, 0.30; working distance, 3.6) and 514-nm excitation. Themounted brain was then inverted and the ventral half was imaged in the sameway. To obtainthe 3.4-mm-thick volumeimage visualizing from the cortexto thethalamus (Fig. 2fl),the intactline-Hmousebrainwas mountedas describedandimaged using the310 objective and 514-nm excitation (stack size, 3.4 mm; stepsize, 2mm). The immunostained 1-mm-thick coronal blocks were incubated inFocusClear for 1 day and enclosed between the coverglass-bottom Petri dish and

    slide glass. The mounted samples were imaged using the3

    10 objective andsingle-photon excitation (514 nm, 591 nm and 654 nm).

    Imaging of CLARITY-processed post-mortem human brain tissue. Clarifiedand immunostained tissues were incubated in FocusClear for 1 day and mountedas described above. The tissues were then imaged using the Leica SP5 systemequipped withthe325 water-immersionobjective (Leica HCX IRAPOL; numeri-cal aperture, 0.95;working distance, 2.4mm; stack size,500 mm; step size, 0.5mm).

    Protein-loss measurement. Six PFA-fixed adult mouse (4 weeks old) brains (for4% SDS, Scale and Triton X-100 treatments) and two (4 weeks old) PFA-fixed,hydrogel-embedded brains (for CLARITY) were cut into 1-mm-thick coronalblocks. One half of each PFA-fixed brain was weighed and placed in 2.5 ml of 4%SDS, ScaleU2 (a mixture of 4 M urea and 30% glycerol) or 0.1% Triton X-100solution. One half of each PFA-fixed, hydrogel-embedded brain was placed in2.5ml of 4% SDS solution. The tissues were allowed to clearfor 1week at 37uC inthe respective solutions and quantity of protein lost from tissue by diffusing intosolution was measured using the BCA (bicinchoninic acid) protein assay; total

    protein inmousebrain wasestimatedat 10%(wt)on thebasis ofpreviouswork45,46.

    Experimental subjects. Animal husbandry and all aspects of animal care andeuthanization as described were in accordance with guidelines from the NationalInstitutes of Health and have been approved by members of the StanfordInstitutional Animal Care and Use Committee. Use of post-mortemhuman tissuewas in accordance with guidelines from the National Institutes of Health andapproved by the Stanford Institutional Review Board.

    Neurite tracing. Manual tracing of individual neurons was performed usingImaris software (Bitplane). Neurons with cell bodies located in the middle150mm of the z-stack were randomly sampled and chosen for tracing. The cell

    body was reconstructed semi-automatically through a user-defined thresholdthat included as much of the cell body as possible but less than 5mm of anydendrite. All neurites originating from the cell body were traced manually inshort segments in the Surpass mode, and each segment was automaticallycentred (with the opportunity for user corrections) before being connected.The number of interconnections between filaments of the same cell, and thenumber of interconnections between a filament from the traced cell and that ofany other cell, was counted manually. Separately, for Fig. 5hn neurons withinteresting structures were chosen non-randomly and traced using the samemethod described above.

    Basic CLARITY protocol. Hydrogel solution preparation: (1) Combine and mix40 ml of acrylamide (40%), 10 ml of bis-acrylamide (2%), 1g of VA-044 initiator(10%wt),40 ml of310PBS, 100ml of16%PFA and 210ml ofdH2O with specialattention to temperature andsafety precautions. Keep allcomponents on iceat alltimes to prevent polymerization. Caution: PFA is an irritant, sensitizer, carcino-gen and toxin. Acrylamide is a potent neurotoxin, a respiratory and skin sen-

    sitizer, carcinogen,irritant,mutagen, teratogen and reproductive hazard.Many ofthechemical constituentsof hydrogelsthat could be used forCLARITY would fallinto oneor more of these categories. Therefore, to avoidskin contactor inhalationof monomers and/or crosslinkers (for example, acrylamide or PFA), solutionpreparation and all subsequent handling of hydrogel solution and polymer mustbe conducted in a fume hood with personal protective equipment including faceshield, laboratory coat, gloves and closed-toe shoes.

    Saponin is a widely used mild non-ionic surfactant often used to permeabilizecellular membranes in conventional immunohistochemistry. In CLARITY,saponin can be used in the hydrogel monomer infusion process to facilitatediffusion of the hydrogel monomer and initiator into the tissue, particularly forsamples in which cardiac perfusion is not feasible, such as post-mortem humantissues and zebrafish brains. Saponin shortens incubation time required in thehydrogel monomer infusion process. However, bubbles may form that could belinked to saponin use, so routine saponin is not suggested. (2) Distribute 40-mlaliquots into 50-ml conical tubes on ice. Each tissue sample will require the useof one 40-ml tube: 20ml for perfusion and the remaining 20ml for sampleembedding. (3) Seal tubes tightly and keep in secondary containment (on ice)beforeremoving them from thehood. Transfer aliquots from iceto220 uC. Storethese solutions at 220 uC until they are ready to be used. They can be stored at220 uC indefinitely if all components were kept sufficiently cold during thepreparation process.

    Solution preparation: (1) Combine and mix 123.66 g boric acid, 400g sodiumdodecyl sulphate, and 9 l dH2O. To avoid skin contact or inhalation, preparesolution in a fume hood in proper PPE. Paying special attention to safety, com-bine water, boric acid and SDS while stirring. Add dH2O to 10 l and add NaOHuntil the pH has reached 8.5. This solution can be made, stored and used at roomtemperature (20 uC). Caution: SDS is a toxin and irritant to the skin and respir-atory system.

    Transcardial perfusion with hydrogel solution: (1) Before perfusing, thaw thefrozen hydrogel monomer solution in the refrigerator or on ice. (2) When the

    solution is completely thawed and transparent (but still ice cold), gently invert tomix. Make sure thatthereis noprecipitate andavoidintroducing anybubbles intothe solution. (3) Prepare perfusion materials within a fume hood. (4) Deeplyanaesthetize adult mouse with Beuthanasia-D. (5) Perfuse the animal transcar-dially, first with 20ml of ice-cold 13 PBS and then 20 ml of the ice-cold hydrogelsolution. Maintain a slow rate of perfusion (about 2 min for the 20 ml of eachsolution). (6) Immediately place the tissue (for example, brain) in 20 ml coldhydrogel solution in a 50-ml conical tube. Keepthe samplein solution on iceuntilit can be moved to a 4 uC refrigerator. (7) Cover sample in aluminium foil if itcontains fluorophores and incubate at 4 uC for 23 days to allow for furtherdiffusion of the hydrogel solution into the tissue.

    Hydrogel tissue embedding: (1) De-gas the 50-ml conical tube containing thesamplein the desiccationchamber (in a fume hood) toreplace all ofthe gas inthetube with nitrogen (as oxygen impedes hydrogel formation), as follows: place the50-ml conical tube on a rack in the desiccation chamber; twist the 50-ml conicaltube open sufficientlyto allow gas exchange; turn on the nitrogen tankand adjust

    the control valve such that the inlet to the desiccation chamber fills with nitrogen

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