Chromatography Columns and Supplies · 2017-05-31 · Chromatography Columns and Supplies Hichrom...

HICHROMChromatography Columns and Supplies

Hichrom Limited1 The Markham Centre, Station RoadTheale, Reading, Berks, RG7 4PE, UK

Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484Email: [email protected] www.hichrom.co.uk

Catalogue 9

LC COLUMNSYMC

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YMC

YMC-Triart phases are based on a multi-layered hybrid material, manufactured using innovative micro-reactor technology. The combination of this layered hybrid particle with the precise microreactor granulation and the multi-stage endcapping procedures, produces a versatile material with outstanding chemical and physical durability, plus excellent chromatographic performance with acidic, basic and neutral compounds.

• Multi-layer hybrid material• Innovative micro-reactor technology• Multi-stage endcapping• High pH and temperature stability• 1.9, 3 and 5μm particle sizes

YMC-Triart

Phase Specifications1

YMC-Triart C18 YMC-Triart C8 YMC-Triart Diol-HILIC

Base Material Organic/inorganic silica Organic/inorganic silica Organic/inorganic silica

Ligand C18 C8 Diol

Particle Size (µm) 1.9, 3, 5 1.9, 3, 5 1.9, 3, 5

Pore Size (Å) 120 120 120

Bonding Polymeric type Polymeric type -

Endcapping Multi-stage hybrid groups Multi-stage hybrid groups -

Carbon Load (%) C18: 16 (20% in total) C8: 7 (11% in total) -

pH Range 1-12 1-12 2-10

Temperature Range pH 1-7:70°C, pH 7-12: 50°C pH 1-7:70°C, pH 7-12: 50°C 50°C

Features of YMC-Triart• Homogeneous and uniform particles. Use of microreactor technology produces homogeneous and uniform particles that result in low operating pressure and reproducible surface modification, leading to excellent column-to-column and lot-to-lot reproducibility.

• Multi-stage endcapping. YMC-Triart uses an innovative endcapping procedure for surface modification. Using a stepwise, multi-stage process with a number of compounds with different reactivities, the maximum number of silanols can be capped.

• Balanced hydrophobicity. YMC-Triart exhibits a balanced hydrophobicity, making it more retentive than other hybrids.

• Excellent durability. YMC-Triart C18 and C8 show excellent chemical stability. Even at high pH (up to pH 12) or high temperature conditions (up to 70°C), the column lifetime is greater than for conventional C18 and C8 columns.

• Method transfer between HPLC ↔ UHPLC. YMC-Triart phases provide matching chromatographic behaviour for all particle sizes. This ensures identical selectivity and excellent peak shape for basic compounds for all particle sizes. YMC-Triart allows predictable scale-up from UHPLC to conventional HPLC and even to semi-preparative LC and vice versa. Figure 1 illustrates method transfer from a 5µm, 150 x 2.0mm YMC-Triart C18 to a 1.9µm, 50 x 2.0mm column. Resolution is maintained but run time has been decreased from 55mins to 6mins.

Figure 1. Method transfer between HPLC and UHPLC

Columns: A: YMC-Triart C18 (5µm, 150 x 2.0mm) B: YMC-Triart C18 (1.9µm, 50 x 2.0mm)Eluent: A: 0.1% formic acid in H

2O

B: 0.1% formic acid in CH3CN

Temperature: 40°CDetection: UV, 240nm

1. Oxine copper2. Asulam3. Thiram4. Triclopyr5. Mecoprop6. Flazasulfuron7. Siduron8. Halosulfuron-methyl9. Azoxystrobin

1 Other Triart phases are available. See page 6

4.5 MPa (650 psi)5% B (0-3 min)5-30% B (3-10 min)30-45% B (10-55 min)0.2 ml/min

37 MPa (5400 psi)5% B (0-0.3 min)5-30% B (0.3-1.0 min)30-45% B (1.0-6.0 min)0.6 ml/min

0 1 2 3 4 5 Mins6

0 10 20 30 40 50Mins 55

1

243

5 6

7

8 9

1

2 4

3

5

6

7

8 9

A

B

55 min

6 min

inorganic siloxane layer

organic siloxane layer

LC Columns – YM

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www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660 267

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YMC-Triart (continued)

Features of YMC-Triart (continued)

• Improvement of loadability. YMC-Triart C18 can tolerate larger injection volumes containing strong solvents (eg. acetonitrile), whilst maintaining better peak shape than traditional silica-based columns. This can be important for difficult to solubilise samples that need higher concentrations of organic solvent for solubilisation prior to analysis. Figure 2 illustrates the influence of injection volume on peak shapes.

• LC-MS compatibility. Extremely low levels of column bleed lead to reduced background noise and hence lower levels of detection. In Figure 3, no bleed is observed in the test of total ion current measured by LC-MS with blank (no column) or with YMC-Triart C18.

Hardware for YMC-Triart 1.9μm columnsYMC-Triart columns packed with 1.9µm particle size phases are provided in PT-hardware, which is compatible with Waters UPLC® systems and Agilent equipment. This enables optimised chromatographic separations to be achieved when using these and other instruments.

Ordering InformationWhen ordering please replace ‘XX’ with TA for Triart C18, TO for Triart C8 and TDH for Triart Diol-HILIC.

1.9μm YMC-Triart C18, C8 and Diol-HILIC Columns4

Column i.d. (mm)Column Length1 (mm) Guard Cartridges2

(5mm length)20 30 50 100 150

2.0XX12SP9-0202PT

£469XX12SP9-0302PT

£454XX12SP9-0502PT

£469XX12SP9-1002PT

£483XX12SP9-1502PT

£528XX12SP9-E5Q1CC

£409

3.0 -XX12SP9-0303PT

£469XX12SP9-0503PT

£469XX12SP9-1003PT

£483-

XX12SP9-E503CC£409

3μm and 5μm YMC-Triart C18, C8 and Diol-HILIC Columns4

Column i.d. (mm) Column Length1 (mm) Guard Cartridges3

(10mm length)3μm Particle Size 50 100 150 250

2.0 XX12S03-0502WT £320 XX12S03-1002WT £320 XX12S03-1502WT £379 XX12S03-2502WT £394 XX12S03-01Q1GC £231

3.0 XX12S03-0503WT £298 XX12S03-1003WT £342 XX12S03-1503WT £387 - XX12S03-0103GC £231

4.6 XX12S03-0546WT £305 XX12S03-1046WT £357 XX12S03-1546WT £417 XX12S03-2546WT £454 XX12S03-0104GC £231

5μm Particle Size 50 100 150 250

2.0 XX12S05-0502WT £312 XX12S05-1002WT £357 XX12S05-1502WT £372 XX12S05-2502WT £394 XX12S05-01Q1GC £231

3.0 XX12S05-0503WT £298 XX12S05-1003WT £335 XX12S05-1503WT £357 XX12S05-2503WT £402 XX12S05-0103GC £231

4.6 XX12S05-0546WT £305 XX12S05-1046WT £350 XX12S05-1546WT £379 XX12S05-2546WT £431 XX12S05-0104GC £2311 Other dimensions available 3 5/pk. Requires holder XPGCH-Q1 (£132) - see p. 272 for further information 2 3/pk. Requires holder XPCHUHP (£364) 4 YMC-Triart Phenyl and PFP also available - please enquire

YMC-Triart preparative 10 and 15µm bulk material also available – please enquire.

Figure 2. Influence of injection volume on peak shapes* Figure 3. LC-MS compatibility*

Column: 5µm, 50 x 2.0mmEluent: A: Water – formic acid (100:0.1) B: CH

3CN – formic acid (100:0.1)

5 to 100% B from 0.5 to 2.5minFlow rate: 0.4ml/minTemperature: 40°CDetection: UV, 275nm

YMC-Triart C18

No column

YMC-Triart C18

Silica-based C18

Solvent for sample: acetonitrileInjection volume: 1.0µl, 1.5µl, 2.0µl, 3.0µl

Column: YMC-Triart C18 (5µm, 50 x 2.0mm)Eluent: A: 0.1% formic acid in H

2O

B: 0.1% formic acid in CH3CN

Gradient: 5% B (0-1min), 5-100% B (1-5min), 100-5% B (10-10.1min), 5% B (10.1- 12.5min)Flow rate: 0.4ml/minTemperature: 40°CDetection: ESI +ve, TIC (mass range 50-1000)

*The comparative separations and data presented here may not be representative for all applications.

1. Propranolol2. Verapamil

Please contact Hichrom for current prices. Tel: +44 (0)118 930 3660 Email: [email protected]

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The ProFamily is YMC’s range of state-of-the-art ultra high purity silica phases (99.999%). They are manufactured using high specification silica with extremely low individual metal ion contents (typically <10ppm). Test certificates supplied for both the bulk material and the column characterise the quality and reproducibility of these products.

• Ultra high purity silica• C18, C8 and C4 bonded phases• Hydrosphere for aqueous stability• ProC18 and Hydrosphere C18 in 2, 3 and 5μm phases

YMC ProFamily

YMC ProFamily Phases

YMC Phase Code DescriptionParticle Size

(μm)Pore Size

(Å)Carbon Load

(%)pH Range Applications

ProC18 ASUltrapure silica base with low residual non-specific interactions

21, 3, 5 120 16 2-8Fat soluble vitamins, antioxidants, acidic, neutral, basic and chelating compounds

ProC18RS RSHigh carbon load with polymeric bonding C18

3, 5 80 22 1-102 Wide range of samples including acidic and basic compounds

Hydrosphere C18 HSCan be used in 100% aqueous eluent

21, 3, 5 120 12 2-8Strongly polar compounds, antibiotics, catecholamines, nucleic acids, water soluble vitamins

ProC8 OSLatest generation C8 with very low residual non-specific interactions

3, 5 120 10 2-7.5Acidic, neutral, basic and chelating compounds, drugs and metabolites

ProC4 BSUltrapure silica base, endcapped

3, 5 120 7 2-7.5Polar acidic, basic and chelating compounds, polar peptides

1 YMC-UltraHT phases 2 It is recommended to use at least 10% organic solvent composition near the pH limits and over 50% at pH levels above pH 9.0 to preserve column lifetimes

Phases in the YMC ProFamily are ideal for applications in both the pharmaceutical and biotechnology industries. ProC18RS is specifically designed for improved retention of non-polar, hydrophobic compounds. The high carbon load and surface area (510m2/g) and extended pH stability provide further selectivity options for demanding separations, including LC-MS and method development. The enhanced steric selectivity of this phase enables positional isomers to be separated. Hydrosphere C18 contains a hydrophilic C18 surface for enhanced polar selectivity and operation under 100% aqueous eluent conditions. Typical applications include nucleic acid bases, nucleotides, organic acids, catecholamines, vitamins etc.

Figure 4 compares the selectivity characteristics of the three C18 phases using hydrophobicity and hydrogen-bonding ability as indicators. This plot highlights the contrasting separation features of these phases. Figure 5 shows a comparison of the differing hydrophobicities and hydrogen bonding capacities of each phase in combination with basic substances.

Figure 4. Comparison of YMC ProFamily separation selectivity

Figure 5. Comparison of characteristics of ProFamily phases

Column: 50 x 2.0mmEluent: CH

3OH - 10mM CH

3COONH

4 (55:45)

Flow rate: 0.2ml/minTemperature: 37˚CDetection: UV at 254nm

1. Toluene2. Amitriptyline HCl3. Progesterone4. Propylbenzene5. Acenaphthene

ProC4

ProC8ProC18

ProC18RS

Hydrosphere C18

0 10 20 30 40 Mins0 10 20 30 Mins0 10 Mins 0 10 20 Mins 0 10 20 Mins

1

2

4

3

5

1

2 5,4

3

1

2

4

3

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12

4

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lowest hydrophobicity balanced hydrogen bonding, capacity and hydrophobicity

lower hydrophobicity highest carbon load high polar recognition

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The YMC-UltraHT ProC18 and Hydrosphere C18 bonded phases are based on high efficiency 2µm spherical silica particles, of the same pore size, surface area and carbon content as the equivalent 3 and 5µm products (see page 268). They are specifically designed for high speed and high throughput analyses in various fields including pharmaceuticals, foods and environmentals. YMC-UltraHT columns exhibit low pressure drop and high flow rates can be achieved without generating excessive back pressure and without the need for specialised equipment.

Due to the production process used to manufacture the YMC ProFamily, methods can easily be downscaled without changes to selectivity. Figure 6 shows the transfer of a separation of a polar isoflavone mixture from a 5µm, 150 x 4.6mm i.d. YMC Hydrosphere C18 column to a 2µm, 50 x 2.0mm i.d. YMC-UltraHT Hydrosphere C18 column.

• ProC18 and Hydrosphere C18 phases• Same selectivity as the corresponding 3 and 5μm phases• Use with UHPLC or conventional HPLC systems• Fully downscalable from 3 and 5μm to 2μm

YMC-UltraHT (2μm)

Ordering Information – YMC ProFamily

YMC Phase Column Dimensions2 (mm) Guard Cartridges1 (5/pk, 10 x 4mm)

3μm 50 x 2.1 100 x 2.1 150 x 2.1 50 x 4.6 100 x 4.6 150 x 4.6 -

ProC18AS12S03-05Q1QT

£335AS12S03-10Q1QT

£335AS12S03-15Q1QT

£402AS12S03-0546WT

£320AS12S03-1046WT

£305AS12S03-1546WT

£446AS12S03-0104GC

£245

ProC18RSRS08S03-05Q1QT

£312RS08S03-10Q1QT

£335RS08S03-15Q1QT

£402RS08S03-0546WT

£312RS08S03-1046WT

£357RS08S03-1546WT

£431RS08S03-0104GC

£245

Hydrosphere C18HS12S03-05Q1QT

£312HS12S03-10Q1QT

£335HS12S03-15Q1QT

£402HS12S03-0546WT

£320HS12S03-1046WT

£372HS12S03-1546WT

£446HS12S03-0104GC

£245

ProC8OS12S03-05Q1QT

£312OS12S03-10Q1QT

£335OS12S03-15Q1QT

£402OS12S03-0546WT

£312OS12S03-1046WT

£357OS12S03-1546WT

£431OS12S03-0104GC

£245

ProC4BS12S03-05Q1QT

£312BS12S03-10Q1QT

£335BS12S03-15Q1QT

£402BS12S03-0546WT

£312BS12S03-1046WT

£327BS12S03-1546WT

£431BS12S03-0104GC

£2455μm 50 x 2.1 150 x 2.1 250 x 2.1 50 x 4.6 150 x 4.6 250 x 4.6 -

ProC18AS12S05-05Q1QT

£327AS12S05-15Q1QT

£387AS12S05-25Q1QT

£431AS12S05-0546WT

£320AS12S05-1546WT

£387AS12S05-2546WT

£454AS12S05-0104GC

£245

ProC18RSRS08S05-05Q1QT

£312RS08S05-15Q1QT

£387RS08S05-25Q1QT

£409RS08S05-0546WT

£312RS08S05-1546WT

£379RS08S05-2546WT

£454RS08S05-0104GC

£245

Hydrosphere C18HS12S05-05Q1QT

£312HS12S05-15Q1QT

£387HS12S05-25Q1QT

£409HS12S05-0546WT

£320HS12S05-1546WT

£402HS12S05-2546WT

£454HS12S05-0104GC

£245

ProC8OS12S05-05Q1QT

£312OS12S05-15Q1QT

£387OS12S05-25Q1QT

£409OS12S05-0546WT

£312OS12S05-1546WT

£379OS12S05-2546WT

£454OS12S05-0104GC

£245

ProC4BS12S05-05Q1QT

£312BS12S05-15Q1QT

£387BS12S05-25Q1QT

£409BS12S05-0546WT

£312BS12S05-1546WT

£379BS12S05-2546WT

£454BS12S05-0104GC

£245

YMC-UltraHT Phase Column Dimensions (mm)2μm 30 x 2.0 50 x 2.0 75 x 2.0 100 x 2.0 150 x 2.0

£537 £512 £529 £537 £678

ProC18 AS12S02-0302WT AS12S02-0502WT AS12S02-L502WT AS12S02-1002WT AS12S02-1502WT

Hydrosphere C18 HS12S02-0302WT HS12S02-0502WT (£521) HS12S02-L502WT HS12S02-1002WT HS12S02-1502WT

30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0

- £521 £529 £537 £711

ProC18 - AS12S02-0503WT AS12S02-L503WT AS12S02-1003WT AS12S02-1503WT

Hydrosphere C18 - HS12S02-0503WT HS12S02-L503WT HS12S02-1003WT HS12S02-1503WT1 Use with universal holder XPGCH-Q1 (£132) or direct connect holder XPGCS-Q1 (£132) – see p. 272. 2.1mm i.d. guard cartridges also available 2 Other dimensions (eg. 1.0mm i.d.) also available

A. Conventional LC method YMC Hydrosphere C18 5μm B. Ultra fast LC method YMC-UltraHT Hydrosphere C18 2μm

Figure 6. Downscaling of isoflavone separation

Column: YMC Hydrosphere C18 (5µm, 150 x 4.6mm)Eluent: A: H

2O - CH

3CO

2H (100:3)

B: CH3CN - CH

3CO

2H (100:3)

Gradient: 15 - 35% B in 30 minsFlow rate: 1.0ml/minTemperature: 35°C

Column: YMC-UltraHT Hydrosphere C18 (2µm, 50 x 2.0mm)Eluent: A: H

2O - CH

3CO

2H (100:3)

B: CH3CN - CH

3CO

2H (100:3)

Gradient: 12.5 - 30% B in 3.3 mins.Flow rate: 0.9ml/minTemperature: 35°C

1. Daidzin2. Glycitin3. Genistin4. 6’-O-Malonyldaidzin5. 6’-O-Malonylglycitin6. 6’-O-Acetyldaidzin7. 6’-O-Acetylglycitin8. 6’-O-Malonylgenistin9. Daidzein10. Glycitein11. 6’-O-Acetylgenistin12. Genistein

0 6 12 18 24 Mins 0 1 2 3 Mins

12

4

3

56 7 8

109

11

121

2

4

3

56 7 8

109

11

12


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YMC manufacture a wide range of ‘classical’ and speciality bonded phases for reversed-phase, normal-phase and HILIC methods. The traditional ODS-A, ODS-AL, ODS-AM and ODS-AQ phases are well documented in the scientific literature. The series of three J’sphere phases offers alternative hydrophobicities due to variation in degree of ODS substitution. The Polyamine ll material is a mixed secondary and tertiary amino derivative, covalently bonded onto 5µm particle size silica. This phase offers increased lifetime and alternative selectivity for carbohydrate applications compared with conventional primary amino materials. The PVA-SIL material is prepared by bonding the silica support with a monomolecular polymer coating of vinyl alcohol. It offers an excellent alternative selectivity to silica and can be used over a wider pH range.

YMC ‘Classical’ Phases

YMC Phase Code DescriptionParticle1 Size (μm)

Pore Size (Å)

Carbon Load (%)

pH Range Applications

Carotenoid CTC30 ligands. Proprietary polymeric bonding chemistry

3, 5 proprietary - 2-6Isomeric carotenes, retinols, steroids, fat-soluble vitamins

ODS-A AA Traditional high performance C18 3, 5120, 200,

30017, 12,

72-7.5

Pharmaceuticals, vitamins, amino acids, peptides, general purpose phase

ODS-AM AMStrict QC controlled production provides high performance C18 column for validated methods

3, 5 120 17 2-7.5Purines, phenols, PTC-amino acids, angiotensins, alkaloids

ODS-AQ AQ

‘Hydrophilic’ endcapping for 100% aqueous eluents, increased retention of polar compounds

3, 5 120, 200 14, 11 2-7.5Strong polar compounds, pharmaceuticals, antibiotics, peptides and proteins, nucleic acids

ODS-AL ALTraditional C18 for ‘mixed-mode’ separations

3, 5 120 17 2-7.5Tocopherols, fat soluble vitamins, disinfectants

J’sphere JH, JM, JLC18 family with different hydrophobicity

4 8022, 14, 9

(JH, JM, JL)1-9 (JH),

2-7.5 (JM, JL)Positional isomers, complexing agents, pharmaceuticals

Polymer C18 PCPolymethacrylate matrix, stable towards shrinking and swelling, wide pH applicability

6 proprietary C18=10% 2-13Phenols, anilines, peptides in high pH, pharmaceuticals, quaternary amines

C8 OCHigh coverage monomeric C8 bonding

3, 5120, 200,

30010, 7,

42-7.5

Proteins and peptides, estrogens, general purpose phase

YMCbasic BASpecifically designed for separation of basic compounds

3, 5 proprietary 8 2-7.5Basic molecules without modifiers, anilines, alkaloids, antidepressants

Phenyl PHMonomeric phenyl bonding, endcapped

3, 5 120, 300 9, 3 2-7.5 Phenols, fullerenes, sweeteners

C4 BUC4, fully endcapped, high coverage monomeric bonding

3, 5120, 200,

3007, 5,

32-7.5

Biological separations, polar compounds

Protein RP PRDesigned to withstand exposure to TFA, good recovery rates

5 proprietary 4 1.5-7.5 Proteins, peptides

C1 TMTrimethylsilane, excellent hydrolytic stability

3, 5 120, 300 4, 3 2-7.5 Water soluble vitamins

PVA-SIL PVPolyvinyl alcohol bonded on silica support, suitable for NP or RP applications

5 120 - 2-9Proteins, phospholipids, retinoids, lipids

Polyamine ll PB

Improved hydrolytic stability over conventional amino phase, increased lifetime, rapid equilibration time

5 120 - 2-9Maltooligosaccharides, tocopherols, nucleotides, sugars

Amino NHPrimary amino, high coverage monomeric bonding chemistry

3, 5 120 3 2-7.5Saccharides, nucleotides, water soluble vitamins

Cyano CNFully endcapped, also useful for SFC applications

3, 5 120, 300 7, 2.5 2-7.5 Proteins, steroids, catechols

Diol DLFor aqueous GPC or normal- phase applications, high recovery for biological material

560, 120, 200, 300

9, 7, 5, 3

2-7.5Peptides, proteins, maltooligosaccharides

Silica SLUltra high purity, high mechanical stability

3, 560, 120, 200, 300

- 2-7.5Small organic molecules, fat soluble vitamins, tocopherols

1 See page 274 for YMC preparative grade materials

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YMC ‘Classical’ Phases (continued)

YMC C18 ODS-A This phase is the traditional workhorse for most reversed-phase applications. Endcapping is exhaustive and this greatly reduces the activity of silanol groups and minimises non-specific secondary interactions.

YMC C18 ODS-AM YMC C18 ODS-AM is a high specification C18 material produced to meet the stringent demands for validated analytical HPLC processes. All columns have to pass additional tests to meet the narrow quality specifications range with regards to selectivity pattern, column resolution, absolute retention and peak symmetry.

YMC C18 ODS-AL This phase is a non-endcapped ODS. Non-endcapping imparts some additional secondary normal-phase adsorption interaction characteristics.

YMC ODS-AQODS-AQ differs from traditional C18 phases in that after initial octadecylsilyl bonding, the material is treated to produce a surface that can be wetted with polar eluents. In highly aqueous eluents ODS-AQ gives longer retention times for polar compounds than conventionally endcapped ODS packings.

YMC Polymer C18Polymer C18 is a reversed-phase material which provides a broad range of solvent choices over a pH range of 2 – 13, due to its resistance to shrinking and swelling. It consists of a highly cross-linked spherical hydrophilic methacrylate polymer which is functionalised with C18 groups. Polymer C18 provides similar, but not identical, selectivity to standard silica-based ODS materials. It has slightly greater retention for aromatic compounds than even highly retentive ODS columns. It is significantly less retentive than styrene/divinylbenzene based HPLC columns.

YMCbasicYMCbasic is an early base deactivated phase, widely used for the analysis of pharmaceutical and other basic compounds. The phase consists of monomerically bonded alkyl chains with different chain lengths of C8 and smaller, leading to high surface coverage. Its selectivity is similar to that expected for conventionally endcapped C8 reversed-phase media.

YMC J’sphereIn the YMC J’sphere series of phases, instead of varying the alkyl group chain length in order to produce phases of different polarity, the surface coverage of C18 is varied to produce three phases of graduated hydrophobicity and hydrogen bonding characteristics. The stepwise decrease of hydrophobicity in the J’sphere ODS-H80, M80 and L80 series is accompanied by a corresponding increase in the H-bonding capacity.

YMC Carotenoid The YMC Carotenoid (C30) phase is very lipophilic with exceptional selectivity towards isomeric structures. The C30 ligands provide sufficient phase thickness to enhance interaction with long chained molecules. Geometrical and positional isomers of conjugated double bond systems can be recognised and resolved. The phase is particularly suited for the separation of carotenoids (see Figure 7). Other potential applications include the separation of prostaglandins and leukotrienes.

Figure 7. Extract of SRM 2383, NIST food standard

Column: YMC Carotenoid (5µm, 250 x 4.6mm)Eluent: A: CH

3OH - MTBE - H

2O (81:15:4)

B: CH3OH - MTBE - H

2O (6:90:4)

Gradient A to B in 90minsFlow rate: 1ml/minTemperature: AmbientDetection: UV, 450nm

1. Lutein 2. Zeaxanthin 3. ß-Cryptoxanthin 4. 13-cis ß-Carotene 5. α-Carotene6. trans ß-Carotene7. 9-cis ß-Carotene8. Lycopene

12

4

3

5

6

7

8

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Ordering Information - YMC ‘Classical’ 120Å Phases

YMC2 Phase Column Dimensions4 (mm) Guard Cartridges1 (5/pk, 10 x 4mm)

3μm Particle Size 50 x 3.0 100 x 3.0 150 x 3.0 50 x 4.6 100 x 4.6 150 x 4.6 -

ODS-AQAQ12S03-0503QT

£339AQ12S03-1003QT

£355AQ12S03-1503QT

£405AQ12S03-0546WT

£339AQ12S03-1046WT

£405AQ12S03-1546WT

£413AQ12S03-0104GC

£273

ODS-AAA12S03-0503QT

£314AA12S03-1003QT

£347AA12S03-1503QT

£388AA12S03-0546WT

£347AA12S03-1046WT

£380AA12S03-1546WT

£405AA12S03-0104GC

£273


J’sphere ODS-L805 JL08S04-0503QT £355

JL08S04-1503QT £455

JL08S04-2503QT £537

JL08S04-0546WT £372

JL08S04-1546WT £496

JL08S04-2546WT £521

JL08S04-0104GC £273

J’sphere ODS-M805 JM08S04-0503QT £355

JM08S04-1503QT £455

JM08S04-2503QT £537

JM08S04-0546WT £372

JM08S04-1546WT £496

JM08S04-2546WT £521

JM08S04-0104GC £273

J’sphere ODS-H805 JH08S04-0503QT £355

JH08S04-1503QT £455

JH08S04-2503QT £537

JH08S04-0546WT £372

JH08S04-1546WT £496

JH08S04-2546WT £521

JH08S04-0104GC £273


ODS-AQAQ12S05-0503QT

£314AQ12S05-1503QT

£380AQ12S05-2503QT

£413AQ12S05-0546WT

£339AQ12S05-1546WT

£405AQ12S05-2546WT

£430AQ12S05-0104GC

£273

ODS-AAA12S05-0503QT

£314AA12S05-1503QT

£380AA12S05-2503QT

£405AA12S05-0546WT

£306AA12S05-1546WT

£372AA12S05-2546WT

£405AA12S05-0104GC

£273

ODS-AMAM12S05-0503QT

£314AM12S05-1503QT

£380AM12S05-2503QT

£413AM12S05-0546WT

£339AM12S05-1546WT

£405AM12S05-2546WT

£430AM12S05-0104GC

£273

ODS-ALAL12S05-0503QT

£339AL12S05-1503QT

£380AL12S05-2503QT

£430AL12S05-0546WT

£339AL12S05-1546WT

£413AL12S05-2546WT

£446AL12S05-0104GC

£273


Polymer C18PC99S06-0503QT

£372PC99S06-1503QT

£496PC99S06-2503QT

£537PC99S06-0546WT

£413PC99S06-1546WT

£545PC99S06-2546WT

£521PC99S06-0104GC

£273

YMC2, 3 5μm PhaseColumn Dimensions4 (mm) Guard Cartridges1

(5/pk, 10 x 4mm)100 x 4.6 150 x 4.6 250 x 4.6

Carotenoid (C30) CT99S05-1046QT £413 CT99S05-1546QT £496 CT99S05-2546QT £554 CT99S05-0104GC £273

C8 OC12S05-1046WT £388 OC12S05-1546WT £413 OC12S05-2546WT £446 OC12S05-0104GC £273

Basic BA99S05-1046WT £413 BA99S05-1546WT £446 BA99S05-2546WT £504 BA99S05-0104GC £273

C4 BU12S05-1046WT £388 BU12S05-1546WT £413 BU12S05-2546WT £446 BU12S05-0104GC £273

TMS TM12S05-1046WT £405 TM12S05-1546WT £405 TM12S05-2546WT £446 TM12S05-0104GC £273

Phenyl PH12S05-1046WT £405 PH12S05-1546WT £405 PH12S05-2546WT £446 PH12S05-0104GC £273

Protein RP PR99S05-1046WT £521 PR99S05-1546WT £521 PR99S05-2546WT £562 PR99S05-0104GC £273

SIL SL12S05-1046WT £347 SL12S05-1546WT £372 SL12S05-2546WT £405 SL12S05-0104GC £273

PVA-SIL PV12S05-1046WT £504 PV12S05-1546WT £479 PV12S05-2546WT £554 PV12S05-0104GC £273

Polyamine ll PB12S05-1046WT £413 PB12S05-1546WT £446 PB12S05-2546WT £504 PB12S05-0104GC £273

NH2 NH12S05-1046WT £405 NH12S05-1546WT £405 NH12S05-2546WT £446 NH12S05-0104GC £273

CN CN12S05-1046WT £405 CN12S05-1546WT £405 CN12S05-2546WT £446 CN12S05-0104GC £273

Diol - - DL12S05-2546WT £504 -1 Use with universal holder XPGCH-Q1 (£132) or direct connect holder XPGCS-Q1 (£132). 3mm i.d. guard cartridge also available 4 Other dimensions (eg 2.1 and 1.0mm i.d.) also available2 60Å, 200Å or 300Å pore sizes also available for selected materials 5 80Å3 Available in 3µm particle size for selected materials

YMC Guard Cartridge Holders For 4.6mm i.d. analytical columns, the universal holder XPGCH-Q1 is required for 10mm length guard cartridges and XPGCH-Q2 for 20mm length guard cartridges (see figure 8). For 1.0 to 4.0mm i.d. guard cartridges, the customer can choose between this universal holder or an integral (direct connect) holder XPGCS-Q1 for 10mm length cartridges and XPGCS-Q2 for 20mm length guard cartridges (see figure 9). The integral holder replaces the inlet endfitting on the column with a hand-tightened holder which allows for a very low dead volume connection.

Figure 8. Universal holder XPGCH-Q1 or XPGCH-Q2 Figure 9. Integral holder XPGCS-Q1 or XPGCS-Q2


LC Columns – YM

C


Hichrom Lim

itedYMC

• Porous and non-porous hydrophilic polymer• Ion-exchange phases• Excellent binding capacity and recovery• High resolution

YMC-BioPro is a series of porous and non-porous ion-exchange phases based on a hydrophilic polymer with low non-specific adsorption. The phases are designed for the analysis and purification of peptides, proteins, nucleic acids and other biomolecules. They also show higher binding capacity and higher recovery of biomolecules compared to conventional ion-exchange columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high efficiency and symmetrical peak shapes.

YMC-BioPro

YMC-BioPro Phases

The porous YMC-BioPro phases show high dynamic binding capacity and excellent recovery, making them useful for semi-preparative separations of proteins and antibodies. Figure 10 shows a loading study for proteins on a YMC-BioPro QA column. Figure 11 shows the high resolution separation of DNA fragments achievable with a YMC-BioPro QA-F column.

YMC Phase QA SP QA-F SP-F

Pore Size (Å) 1000 1000 Non-porous Non-porous

Particle Size (μm) 5 5 5 5

Charged Group -CH2N+(CH

3)3

-CH2CH

2CH

2SO

3-CH

2N+(CH

3)3

-CH2CH

2CH

2SO

3

Counter Ion Cl- Na+ Cl- Na+

pH Range 2-12 2-12 2-12 2-12

Dynamic Binding Capacity 110-150mg BSA/ml resin 70-100mg human IgG/ml resin 12-20mg BSA/ml resin 10-18mg human IgG/ml resin

Ordering Information1

Column Dimensions (mm) YMC-BioPro Phase

QA SP QA-F SP-F

30 x 4.6 - - QF00S05-0346WP £760 SF00S05-0346WP £760

50 x 4.6 QAA0S05-0546WP £926 SPA0S05-0546WP £926 QF00S05-0546WP £893 SF00S05-0546WP £893

100 x 4.6 QAA0S05-1046WP £992 - QF00S05-1046WP £1,017 SF00S05-1046WP £1,0171 Preparative grade YMC-BioPro also available as bulk material

Figure 10. Loading study for YMC-BioPro QA

Column: YMC-BioPro QA (5μm, 50 x 4.6mm)Eluent: A. 20mM Tris-HCl (pH 8.1) B. 20mM Tris-HCl (pH 8.1) containing 0.5M NaCl 10-80% B over 30 minutesFlow rate: 0.5ml/minTemperature: 25°CDetection: UV, 280nm

1. Ovalbumin2. Trypsin inhibitor (soybean)

Figure 11. DNA fragments analysed on YMC-BioPro QA-F

Column: YMC-BioPro QA-F (5μm, 100 x 4.6mm)Eluent: A. 20mM Tris-HCl (pH 8.1) containing 0.5M NaCl B. 20mM Tris-HCl (pH 8.1) containing 1.0M NaCl 40-100% B over 30 minutesFlow rate: 0.5ml/minTemperature: 25°CDetection: UV, 260nm

1kb DNA ladder (75-12,216 bp)

0 10 20 30Mins

500µg

250µg

100µg

50µg

0 5 10 15 20Mins

1

2


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YMC Capillary Columns

YMC manufacture a range of LC-MS compatible nano and capillary columns of 75, 100, 300 and 500μm i.d. and lengths of 50, 75, 100 and 150mm. For highly contaminated samples, the use of pre-columns is recommended. These connect to the capillary column with a pre-column coupler which is included. Pre-columns can also be used as trapping columns.

YMC Preparative Separations

YMC preparative columns, packed with a variety of YMC materials, are available in a wide range of column sizes to accommodate virtually any preparative separation and direct scale-up from analytical and semi-preparative separations. Standard column dimensions range from 50 to 200mm i.d. and 250 to 1000mm in length. Matching YMC preparative guard columns are available. Please contact Hichrom for further information.

Figure 12. Capillary column with pre-column attached

YMC Chiral Columns

YMC manufacture a range of chiral columns, based on different ligand types, suitable for a wide range of chiral separations. See also page 5 for the new YMC CHIRAL polysaccharide series of columns.

YMC Chiral PhasesYMC Phase Ligand Particle Size (μm) Pore Size (Å) Recommended pH Range

Chiral NEA (R) or (S) 1-Naphthylethylamine 5 300 2 - 6.5 (RP)

CD BR (α, β or γ) Bromide derivative of cyclodextrin (α, β or γ) 5 120 3.5 - 6.5

Sumichiral OA Series Various – 17 different types 5 120 -

YMC Chiral NEA (R) and (S)YMC Chiral NEA consists of 1-naphthylethylamine bonded to wide pore spherical silica. (R) and (S) versions are available for elution order reversal. YMC Chiral NEA columns can be used in normal-phase and reversed-phase modes.

YMC Chiral CD BRYMC Chiral CD BR phases consist of bromide derivatives of α-, β- or γ-cyclodextrin, in which the primary hydroxyl groups at carbon 6 are substituted for bromine. These phases are used in reversed-phase mode to separate a wide range of polar, water-soluble compounds and positional isomers of substituted aromatic compounds.

YMC Sumichiral OASeventeen different phases of Sumichiral OA columns are available. These are based on different types of covalent linkage, including amide, urea, ligand exchange type, cyclodextrin type and crown ether phases.

Please contact Hichrom for ordering information for YMC chiral columns.

• Diol • PVA-Sil• Amino • Polyamine ll• Cyano • Silica

For preparative SFC, YMC has developed special column hardware in order to meet the highest demands in quality, safety and reliability.

Please contact Hichrom for further information on YMC SFC columns.

YMC SFC Columns

In addition to its application in enantiomer purification, SFC is gaining importance in the separation of achiral compounds. YMC offer six different selectivity phases for achiral SFC:

YMC Chiral β-CD BR

LC Columns – YM

C


Hichrom Lim

itedYMC

• Purification and isolation of maximum sample quantity• Savings in time and costs• Excellent durability and efficiency

YMC-Actus semi-preparative/preparative columns use modified hardware and packing technology. These columns are specifically designed for fast semi-preparative/preparative chromatography and are available in a range of dimensions from 50 x 20mm i.d. to 250 x 50mm i.d. A uniform packing density results in a longer lifetime than conventional semi-preparative/preparative columns. YMC-Actus columns provide reliable results, even after exposure to severe, rapid gradient conditions and multiple injections. Figure 13 illustrates the excellent durability and efficiency of the purification of glycyrrhizic acid from a herbal medicine achieved under fast gradient conditions at high flow rate.

YMC-Actus for Fast Semi-preparative/Preparative Separations

Ordering Information1 (5μm, 50 x 20mm i.d. Columns)Phase Pore Size (Å) Part Number Price

Triart C18 120 TA12S05-0520WX £959

Triart C8 120 TO12S05-0520WX £959

ProC18 120 AS12S05-0520WX £959

Hydrosphere C18 120 HS12S05-0520WX £959

Phase Pore Size (Å) Part Number Price

ProC18RS 80 RS08S05-0520WX £959

ODS-A 120 AA12S05-0520WX £959

ODS-AQ 120 AQ12S05-0520WX £901

ProC8 120 OS12S05-0520WX £9591 Other phases and dimensions available. Please contact us for details.

YMC supply a wide range of bulk media for scale-up to preparative dimensions and beyond. These include materials for reversed-phase, normal-phase, SEC, ion-exchange and chiral. A selection of media is listed below. Please contact us for media not listed.

Material Particle Size (μm) Pore Size1 (Å)

Triart Prep C18, C8 10, 15, 20, 50 120, 200

ProC18 10 120

ODS-A-HG, ODS-AQ-HG, C8-HG, C4-HG, NH2-HG, CN-HG, Diol-HG, SIL-HG, Ph-HG, TMS-HG 10, 15, 20, 50 80, 120, 200, 300

Diol 10, 15, 20 120, 200, 300

BioPro QA, SP 10, 30, 75 1000

Chiral Prep CD ST and CD PM 10, 20, 50 120

Chiral Prep NEA 10 300HG stands for ‘High Grade’ 1 Not all combinations of particle size and pore size are available

Eluent: A: Water – acetic acid (99:1) B: Methanol – acetic acid (99:1)Gradient: 20% B (0 – 2mins), 20-45% B (2 – 7 mins), 45% B (7 – 10mins)Temperature: Ambient Detection: UV, 260nmSample: Water – methanol – acetic acid extract of commercially available herbal medicine (0.1g/ml)

YMC-Pack ODS-AQ, 50 x 4.6 mm i.d., 5μm3ml/min, 10μl injection

0 5 10 Mins

Figure 13. Purification of glycyrrhizic acid from herbal medicine

0 5 10 Mins

After purification

99.5%

Analysis

Purification

YMC-Actus ODS-AQ, 50 x 20 mm i.d., 5μm60ml/min, 500μl injection

Glycyrrhizic acid

Glycyrrhizic acid

Glycyrrhizic acid

0 5 10 Mins

Bulk Material


Chromatography Columns and Supplies · 2017-05-31 · Chromatography Columns and Supplies Hichrom...

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