Capture the Essence - Laserchrom · Capture the Essence HPLC Columns and Instruments 2008 – 2010....

Capture the Essence

HPLC Columns and Instruments 2008 – 2010

http://www.shodex.com/

We provide a wide range of over 800 different products to meet your analytical needs,

from pretreatment and separation columns to HPLC related instruments.

Please visit our website to see actual application data and uses.

[Caution]

1. Please read the operation manual included with each product carefully before use.

2. For improvement purposes, some specifications are subject to change without notice.

3. The figures included in this catalogue are not guaranteed and should be used only as reference.

4. It is essential to take normal precaution when handling reagents and other chemical products even if safety

information is not included in the operation manual.

5. Products described in this brochure are not intended for clinical use.

Shodex website

Type of columns, packing material, functional group, ligandColumn selection by sample character and separation modeColumn selection by application

Polymer-based Packed Columns for Reversed Phase Chromatography : AsahipakPolymer-based Packed Columns for Reversed Phase Chromatography : RSpak and OthersPolymer-based Packed Columns for Normal Phase ChromatographySilica-based Packed Columns for Normal and Reversed Phase ChromatographyColumns for Cation Exchange ChromatographyColumns for Anion Exchange ChromatographyColumns for Hydrophobic Interaction ChromatographyColumns for Ion Chromatography (Cation analysis)Columns for Ion Chromatography (Anion analysis)Columns for Chiral SeparationColumns for Saccharides analysisColumns for Organic acids and Amino acids analysisColumn selection for Size Exclusion Chromatography (SEC)Applicability of SEC(GPC) columns to solvent replacementAqueous SEC(GFC) columns : Silica-basedAqueous SEC(GFC) columns : Polymer-basedMulti mode columnsAqueous/Organic SEC columnsOrganic SEC(GPC) columns : General AnalysisOrganic SEC(GPC) columns : Rapid Analysis, High performance analysisOrganic SEC(GPC) columns : HFIP solvent typeHigh temperature/Ultra high temperature analysis SEC(GPC) columnsOrganic SEC(GPC) columns : Linear calibration typeSolvent-peak separation columns for organic SEC (GPC)Columns for LC/MS and Rapid analysis of drugsColumns for Column Switching methodAffinity Chromatography columnsColumns for Exclusive UseColumns for Sample Clean-up

Semi-micro and micro columns

10~50mm I.D.Special sizes

Calibration Standards for SEC

Refractive Index Detector : Shodex RI-201, RI-201HRefractive Index Detector : Shodex RI-100 seriesEluent Degassing Device

Column Trouble Shooting ; Baseline, PressureUSP Column ListEP Column ListColumn WashingGeneral precautions for handling a columnHPLC System Trouble Shooting : Baseline, NoiseTechnical information list

Index by product name

234

68

101214161618202224283031323436384042444446464850525660

64

7681

82

848586

55626383838790

88

Column selection

Standard analysiscolumns

Preparative columns

Instruments

Information

Index

Calibration standards for SEC

Semi-micro and microcolumns

CONTENTS

2

Typ

es o

f colu

mns, p

ackin

g m

ate

rial, fu

nctio

nal g

rou

p, lig

an

dC

olu

mn se

lectio

n

Reversed & Normal

phase

(Polymer-based)

Reversed & Normal

phase

(Silica-based)

Cation-Exchange

Anion-Exchange

Hydrophobic

Ion Chromatography

Chiral Separation

Analysis of

Saccharides

Analysis of Organic acids

Aqueous SEC (GFC)

Multi Mode

Aqueous/Organic SEC

Organic SEC (GPC)

Affinity

Column Switching

Sample Clean-up

Asahipak

Asahipak

Asahipak

RSpak

RSpak

RSpak

RSpak

RSpak

Asahipak

RSpak

ODSpak

Silica

Silicapak

Silica

Silica

Silica

Silica

Silica

Silica

Silica

Silica

IEC

IEC

IEC

Asahipak

IEC

IEC

IEC

Asahipak

AXpak

HIC

IC

IC

IC

IC

IC

IC

ORpak

ORpak

ORpak

ORpak

ORpak

SUGAR

SUGAR

SUGAR

SUGAR

SUGAR

USPpak

EP

RSpak

CXpak

OHpak

PROTEIN

RSpak

RSpak

Asahipak

Asahipak

Asahipak

GPC

AFpak

MSpak

MSpak

Asahipak

MSpak

CLNpak

CLNpak

ODP-50, ODP-40

C8P-50

C4P-50

ODP2 HP

RP18, DS

DE, GOLF, CARB

DM-614

NN

JJ-50

NH2P-50

DC-613

F

C18M, C18P

E-411

5SIL

5C8

5C4

5TMS

5CN

5NH

5NPE

5PYE

SP-825

SP-420N

CM-825

ES-502C

QA-825

DEAE-825

DEAE3N

ES-502N

WA-624

PH-814

YS-50

YK-421

Y-521, T-613, R-613

NI-424, I-524A

SI

VH-anion

CDA-453 HQ

CDB-453 HQ

CDC-453 HQ

CDBS-453

CRX-853

SH

SC

SP0810

SZ5532

KS-800

MN-431

SC1011-7F

KC-811

P-421S

SB400

SB-800 HQ

KW400

KW-800

NN

JJ-50

GS-HQ

GS

GF-HQ

KF-800, KF-600, KF-400HQ, K-800, KD-800, HFIP-800, HFIP-600, LF, HT-800, UT-800, AT-806MS

32 kinds

PK

GF-310, GS-320

ODP-51

SP-80

EV

PAE

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyhydroxymethacrylate

Styrene divinylbenzene copolymer

Polymethacrylate



Polyvinyl alcohol

Polyvinyl alcohol


Silica

Silica

Silica

Silica

Silica

Silica

Silica

Silica

Silica

Silica

Silica




Polyvinyl alcohol




Polyvinyl alcohol



Polyvinyl alcohol

Silica



Polyvinyl alcohol

Polyvinyl alcohol




Silica













Silica

Silica


Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol



Hydrophilic copolymers containing N-vinyl acetoamide

Polyvinyl alcohol

Polyvinyl alcohol



Polyvinyl alcohol

6, 48

6

6

8, 48

8

8, 28, 48, 56, 58

8

8, 28, 48, 58

8, 48, 58

10, 25, 48

10

12

12, 58

12

12

12

12

12

12

12

12

12

14

14

14

14

16

16

16

16

16

16

18

18

18

20

20, 58

20, 58

22

22

22

22

22

24

24, 25

24

25

25

24, 56

24, 56

28, 58

28

34

34, 56

32

32

8, 28, 48, 58

8, 44, 58

25, 36

56

38, 58

40, 42, 44, 46, 48

22, 48, 56

50, 56

48, 50, 58

50

50

60

60

Octadecyl

Octyl

Butyl

—

—

—

—

Sulfo

Quaternary ammonium

Amino

Sulfo(Na+)

Octadecyl

Octadecyl

—

—

Octyl

Butyl

Trimethylsilyl

Cyanopropyl

Aminopropyl

Nitrophenylethyl

Pyrenylethyl

Sulfopropyl

Sulfopropyl

Carboxymethyl

Carboxymethyl

Quaternary ammonium

Diethylaminoethyl

Diethylaminoethyl

Diethylaminoethyl

Diethylaminoethyl

Phenyl

Carboxyl

Carboxyl

Sulfo

Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

α-Cyclodextrin derivative

β-Cyclodextrin derivative

γ-Cyclodextrin derivative


L-amino acid derivative

Sulfo

Sulfo(Ca2+)

Sulfo(Pb2+)

Sulfo(Zn2+)

Sulfo(Na+)

Sulfo(Ca2+)

Sulfo(Ca2+)

Sulfo

Sulfo(Na+)

—

—

Hydrophilic polymer

Hydrophilic polymer

Sulfo

Quaternary ammonium

—

—

—

—

32 kinds of ligand

—

—

Octadecyl

Sulfopropyl

—

—

Separation Type Products Base Material Functional Group Page

Types of columns, packing material, functional group, ligand

Column selection by sample character and separation mode

3

Colu

mn se

lectio

nC

olu

mn se

lectio

n b

y sam

ple

chara

cte

r and se

para

tion

mod

e

Aqueous-soluble

≧ 2,000

≧ 2,000

Size Exclusion

Organic-soluble

Size Exclusion

≦ 2,000

Size Exclusion

Reversed Phase

Normal Phase

≦ 2,000

Size Exclusion

Ligand Exchange

Ion Exclusion

Ion Exchange

Affinity

Normal Phase

Chiral Separation

Ion Chromatography

Reversed Phase

Ion Exchange

Ligand Exchange

Ion Exclusion

Hydrophobic Interaction

Reversed Phase

Affinity

KW series, SB series, GF series, GS series, KS series

KF series, K series, KD series, LF series, HFIP series, GF series, (SB series), UT series, HT series, AT-806MS

KF series, K series, KD series, LF series, GF series, (SB series)

DE series, DS series, DM-614, C18M, C18P, ODSpak, 5C8, 5C4, 5TMS

5SIL, 5NH, Silicapak

KW series, SB series, GF series, GS series, KS series

SC1011, SC1821, SC1211, SP0810, KS-801, KS-802, SZ5532, MN-431

KC-811, SH1011, SH1821

P-421S, WA-624, NH2P-50, NN series, JJ-50

NH2P-50, DC-613, SZ5532, SC1211, 5SIL, 5NH, Silicapak

AFpak

CDA-453 HQ, CDB-453 HQ, CDC-453 HQ, CDBS-453, CRX-853, ABA-894

NI-424, I-524A, Y-521, YK-421, YS-50, SI-90, SI-50, SI-91, SI-52, VH-anion, T-521, R-621

ODP2 HP, ODP-40, ODP-50, C8P-50, C4P-50, DE series, RP18-413, RP18-613, DS seriesC18M, C18P, ODSpak, 5C8, 5C4, 5TMS, 5CN

IEC series, ES series, WA-624

SC1821, KS-802

SH1821

PH-814

ODP-40, ODP-50, C8P-50, C4P-50, RP18-415

AFpak

24, 25, 32, 34, 36, 38, 48, 50, 56, 58

34, 38, 40, 42, 44, 46, 48, 50, 58

34, 38, 40, 42, 46, 48, 50, 58

8, 12, 18, 48, 56, 58

12

24, 32, 34, 36, 38, 48, 50, 56, 58

24, 25

24, 28, 58

8, 110, 16, 25, 28, 48, 56, 58

22, 52, 56

10, 12, 25

22

18, 20, 58

6, 8, 12, 18, 48, 50, 56, 58

14, 16

24

24

16

6, 8, 48, 50

22, 48, 56

Sample Solubility Sample MW Separation mode PageColumn

4

Colu

mn se

lectio

n b

y applic

atio

n N

ew

Mate

rials E

nviro

nm

en

t Bio

tech

nolo

gy, L

ife S

cie

nces

Colu

mn se

lectio

n

Column selection by application

New Materials

Environment

Biotechnology, Life Sciences

6, 8, 12, 48

38, 40, 42, 48

34, 38, 40, 48

44

20, 58

20

18

18

20, 58

20, 58

18

18

18

38, 48, 50, 58

12, 58

8, 48, 58

60

6, 8, 12, 48, 50

14, 16

6, 8, 48, 50

14, 16

14, 16

6, 8, 48, 50

16

52

52

52

36

6, 8, 48, 50

16

52

36

6, 12, 48, 50

16

34, 38, 48, 50

52

12

38, 42, 48, 50

6, 48, 50

34, 38, 48

Additives, Oligomer

Ion

Peptide

Protein

Nucleic acid

Phospholipid

Steroid

Surfactant

Pretreatment of PCBs, benzopyrene(GPC clean-up)

Synthetic polymer

Organic-soluble

Polar-organic soluble

Aqueous-soluble

Organic-soluble

Polar-organic soluble

High melting temperature

Organic-soluble

Anion

Low molecular

Glycoprotein

Protease

Oligo nucleic acid

DNA · RNA

Lipoprotein, Blood coagulation factor

Middle size molecular or Polymer

Multi mode

Reversed phase

Multi mode

Size exclusion

Reversed phase

Ion exchange

Reversed phase

Ion exchange

Size exclusion

Size exclusion

Ion exchange

Reversed phase

Hydrophobic interaction

Affinity

Affinity

Affinity

Multi mode

Reversed phase

Ion exchange

Affinity

Multi mode

Reversed phase

Ion exchange

Size exclusion

Affinity

Normal phase

Size exclusion

Reversed phase

Size exclusion

Oxyhalides

Transition metal ion

Rare earth ion

Anionic

Nonionic, Anionic, Cationic

Nucleobase,Nucleotide,Nucleoside

Alkali metal ion, Alkaline earth metal ion, Alkylamine

ODP2 HP, ODP-40, ODP-50, DS series, C18M, C18P, ODSpak

KF series, K series, GF series

KD series, SB series, GF series

SB series, GF series, GS series, KW series

LF series, KF series, K series, HFIP series, GF series

KD series, SB series, GF series

AT-806MS, HT series, UT series

SB series, GF series, GS series, KW series

SI-90 4E, SI-50 4E, SI-52 4E

I-524A, NI-424

YS-50

YS-50, Y-521, YK-421

SI-91 4C

SI-52 4E

T-521

YS-50

R-621

GF-310

C18M

JJ-50

EV series, PAE series

RP18-415, ODP-40, ODP-50, C8P-50, C4P-50, C18M, C18P, ODSpak


RP18-415, ODP-40, ODP-50, C8P-50, C4P-50


KW series, SB series, GF series, GS series

KW series, SB series, GF series, GS series


RP18-415, ODP-40, ODP-50, C8P-50, C4P-50

PH-814

ACA-894, AGA-894, ALC-894, ARC-894

AHR-894, ADS-894

APH-894, AAB-894, AAP-894, ALS-894, AST-894

GS-320

ODP2 HP, DE series, OODP-40, ODP-50, C8P, C4P

WA-624

AAF-894, AED-894, AIA-894, APB-894

GS-320

ODP-40, ODP-50, C8P, C4P, C18M, C18P, ODSpak

ES-502N, DEAE-825, DEAE3N

SB series, GF series

AAF-894, ALS-894

SIL

GF-310, KF-402HQ

ODP-40, ODP-50

SB-802.5 HQ, SB402.5-4E, GF-310

Reversed phase

Size exclusion

Size exclusion

Ion chromatography(suppressor method)Ion chromatography(non-suppressor method)Ion chromatography(suppressor method)Ion chromatography(non-suppressor method)Ion chromatography(post column method)Ion chromatography(suppressor method)Ion chromatography(post column method)

Ion chromatography(post column method)

Ion chromatography(non-suppressor method)

Separation mode Column

32, 34, 36, 40, 4838, 40, 42, 44, 46, 4834, 40, 42, 44, 46, 48

32, 34, 36, 38, 48

25, 32, 38, 42, 43, 48, 5025, 32, 38, 42, 43, 48, 50

Page

Separation mode Column Page


5

Colu

mn se

lectio

nC

olu

mn se

lectio

n b

y applic

atio

n F

oods M

ed

icin

e, C

osm

etic

, Meta

bolite

, Op

tically a

ctive

mate

rial

Column selection by application

Foods24, 25, 56

10, 25

24, 25, 56

24, 34

52

8, 24, 28

20

8, 48

6, 8, 48

10

8, 36, 48

6, 48

12

40, 42

8, 12, 48

12

40, 42, 48

56

28

28

6, 8, 12

10

60

4, 6, 8, 12, 48

8, 48

4, 36, 38, 48

8, 48

22

22, 52

22

8, 12, 48

12

38, 42, 48

12

38, 42, 48

Saccharide

Medicine, Metabolite

Optical active material

Fatty acid, Fatty acid amide

Glycerin fatty acid ester

Medicine, Metabolite in bio-fluid(serum · plasma · urine)

Organic acid

Vitamin

Fatty acid

Nucleic acid

Amino acid

Food additive

Pretreatment of residual pesticides(GPC clean-up)

Monosaccharide, Disaccharide Sugar alcohol

Oligosaccharide

Polysaccharide

Water-soluble

Fat-soluble

Ligand exchange

Normal phase

Size exclusion

Size exclusion

Affinity

Ion exclusion

Ion chromatography

Reversed phase

Reversed phase

Normal phase

Multi mode

Reversed phase

Normal phase

Size exclusion

Reversed phase

Normal phase

Size exclusion

Multi mode

Ion exchange

Multi mode

Reversed phase

Normal phase

Size exclusion

Reversed phase

Multi mode

Multi mode

Reversed phase

Chiral (Ligand exchange)

Chiral (Protein)

Chiral (host-guest type)

Reversed phase

Normal phase

Size exclusion

Normal phase

Size exclusion

NH2P-50, SZ5532, SC1211, DC-613

SB series, KS series

ACA-894, AGA-894, ALC-894, ARC-894

KC-811, SH1011, SH1821, NN series

SI-90 4E, SI-50 4E

ODP2 HP, DE series

ODP2 HP, DE series, ODP-40, ODP-50

NH2P-50

NN series, GS-320

ODP-40, ODP-50

SIL

KF-801, KF401HQ

DE series, RP18 series, C18M, C18P, ODSpak

SIL

GF-310, KF-402HQ

GS-320

P-421S

NN series

NH2P-50

EV series, PAE series

NN series

GF-310, GS-320, PK

ODP2 HP

CRX-853

ABA-894

CDA-453 HQ, CDB-453 HQ, CDC-453 HQ, CDBS-453

DE series, RP18 series, C18M, C18P, ODSpak

SIL

GF-310, KF-402HQ

SIL

GF-310, KF-402HQ

Medicine, Cosmetic, Metabolite, Optically active material



SP0810, SC1011, SC1821, KS-801, KS-802, SC1211, SZ5532

GS-220, SB-802 HQ, SB-802.5 HQ, SB401-4E,SB402.5-4E, KS-801, KS-802

ODS, ODP2 HP, DE series, ODP-50, C8P-50, C4P-50, DS series, C18M, C18P, ODSpak

ODP2 HP, DE series, ODP-40, ODP-50, C8P-50, C4P-50, DS series, C18M, C18P, ODSpak

Large

Small

High

SaccharidesSugar alcohols

Low molecular weight substances,medicines, organic acids, food additives, ets.

Polycyclicaromatic

compounds

Proteins

Peptides

Vitamins

LowPolarity of packing materials

Po

re s

ize

of

pac

king

mat

eria

ls

SilicapakSIL

5NH 5CN 5TMS 5C4 5C8 ODS

NH2P-50DC-613

DM-614 DS-413, 613RP18-413, 613

C4P-50 C8P-50

ODP2 HPDE

ODP-40, 50

RP18-415

6

Polymer-based Packed Columns for Reversed Phase Chromatography : Asahipak

Features

• The large pore size supports the analysis of proteins, peptides, and amino acids

• Wide pH range (pH2 to 13)

• Supports use in 100% water

• Especially good for the analysis of basic molecules

• Higher performance type of ODP-50

ODP-50, C8P-50, C4P-50

ODP-40

Polym

er-b

ase

d P

acked C

olu

mns fo

r Reve

rsed

Ph

ase

Ch

rom

ato

gra

ph

y : Asa

hip

ak

Sta

ndard

analysis

colu

mns

News No.1

F7621001

F7621002

F7620002

F7620001

F6710001

F6710023

F7620004

F7620003

F6710022

F7620009

F6713001

F7620006

F7620005

F6710002

F7620008

F7620007

F6710003

Asahipak ODP-40 4D

Asahipak ODP-40 4E

Asahipak ODP-50 6D

Asahipak ODP-50 6E

Asahipak ODP-50G 6A

Asahipak ODP-50 4B

Asahipak ODP-50 4D

Asahipak ODP-50 4E

Asahipak ODP-50G 4A

Asahipak ODP-50 2D

Asahipak ODP-50G 2A

Asahipak C8P-50 4D

Asahipak C8P-50 4E

Asahipak C8P-50G 4A

Asahipak C4P-50 4D

Asahipak C4P-50 4E

Asahipak C4P-50G 4A

4

4

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

H2O/CH3CN=35/65

Column Size(mm) I.D. x L

4.6 x 150

4.6 x 250

6.0 x 150

6.0 x 250

6.0 x 10

4.6 x 50

4.6 x 150

4.6 x 250

4.6 x 10

2.0 x 150

2.0 x 10

4.6 x 150

4.6 x 250

4.6 x 10

4.6 x 150

4.6 x 250

4.6 x 10

Plate Number(TP/column)

Product NameProduct

CodeParticle Size

(μm)

250

250

250

250

—

250

250

250

—

250

—

250

250

250

250

250

250

Pore Size(Å)

≧ 11,000

≧ 17,000

≧ 9,000

≧ 14,000

(guard column)

≧ 2,500

≧ 9,000

≧ 14,000

(guard column)

≧ 5,000

(guard column)

≧ 7,000

≧ 11,000

(guard column)

≧ 6,000

≧ 9,000

(guard column)

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octyl

Octyl

Octyl

Butyl

Butyl

Butyl

FunctionalGroup

Shipping Solvent

Standard Columns

* See page 64 for the semi-micro columns and micro columns for ODP-40.* See page 76 for the preparative columns.* Contact Shodex or our distributors near you for custom columns.

Base Material : Polyvinyl alcohol

Characteristics and Applications of Packing Materials for Normal & Reversed Phase Columns

0 5

5

2 4 6 8 10 12 14

10

15

10 15 20 25 min

elut

ion

volu

me

(mL)

carbon number (n)

EG

EG

EG

C4

C4

C4

C6

C6

C6

C8

C8

C8

C10

C10

C10

C12

C12

C12

C14

C14

C14

C4P-50

C4P-50

C8P-50

C8P-50

ODP-50

ODP-50

ODS-A

25m

M10

mM

1mM

0mM

ODS-B

0 10

0 min min10

0 10

0 10

0 10

adsorption

0 10

adsorption

ODP-50

min

0 10

0 10

0 10

0 10

pH3 pH7 pH11

0 20 min 0 20 min 0 20 min

1 1 1

3

3

3

2

22

1. Benzocaine

Sample :

H2N COOC2H5

2. Lidocaine

3. Tetracaine

CH3

C2H5

C2H5

CH3

COOCH2CH2N

NH(CH2)3CH3

NHCOCH2N

CH3

CH3

0 0

0 10 20 min

10 min

2

2

23

4

5 8

9

10

1112

6

7

1

1

13

4

5

67

5 10 min

CH2 CH

NH2

COOH

20%CH3CN

50%CH3CN

NH

N

CH2 CH

NH2CH3

COOHN

N

7

Sample : Ethyleneglycol, n-alkylalcohol

Column :Shodex Asahipak ODP-50 4D C8P-50 4D C4P-50 4DEluent :H2O/CH3OH=20/80Flow rate :0.6mL/minDetector :RIColumn temp.:30˚C

Alkylalcohols

Relationship between the Number of Carbon Atoms of Alkylalcohols and Elution Volume

Effect of Salt Concentration in the Analysis of Basic substance

Sample : Scopolamine 1.0mg/mL, 50μL

Column :Shodex Asahipak ODP-50 4D ODS-A ODS-BEluent :Phosphate buffer (pH7.0)/CH3CN=60/40Flow rate :0.6mL/minDetector :UV(254nm)Column temp.:30˚C

When using an ODS column, nonspecific adsorption of samples is observed under low ionic concentration conditions, and complete adsorption without any elution is achieved under the absence of coexisting ions.However, when using ODP-50, a polymer-based column, the sharp peak of scopolamine is observed regardless of the ionic concentration, even under the absence of coexisting ions, suggesting that the problematic nonspecific adsorption has been eliminated.The ODP columns are suitable for analysis using the ESI method (LC/MS) that is easily interfered with by the presence of salt.

Local Anesthetics

Column :Shodex AsahipakODP-50 4DEluent :25mM Phosphate buffer/CH3CN=60/40Flow rate :0.6mL/min Detector :UV(254nm) Column temp.:30˚C

Dissociation of tertiary amino groups contained in basic chemical substances can be suppressed by making the pH of the eluent higher than the pKa of the amino groups.This increases the relative hydrophobicity of the chemical substances, thereby allowing the column to hold the chemical substances more strongly and to separate them more clearly.

Sample : 25μg/mL each, 10μL

1. Histidine

Sample : 40μLSample : 6μL1. Substance P frg.1-4 25μg/mL2. Glu-Gly-Phe 25μg/mL3. Leu-Trp-Met-Arg 15μg/mL4. FMRF-Amide 20μg/mL5. Met-Enkephalin 15μg/mL6. α-Endorphin 30μg/mL7. Des-Arg9-Bradykinin 40μg/mL

Histidine and 3-Methylhistidine Peptides Gradient Analysis of Proteins and Peptides

Column :Shodex Asahipak ODP-50 6D Eluent :10mM Sodium phosphate buffer(pH7.0)Flow rate :0.5mL/minDetector :UV(210nm)Column temp.:30˚C

Column :Shodex Asahipak ODP-50 4D Eluent :0.05% TFA aq./CH3CN=82/18Flow rate :0.6mL/minDetector :UV(220nm)Column temp.:30˚C

Column :Shodex Asahipak ODP-50 6D Eluent : (A); 0.05% TFA aq./CH3CN=80/20 (B); 0.05% TFA aq./CH3CN=50/50 Linear gradient; (A) to (B), 20minFlow rate :1.0mL/minDetector :UV(220nm)Column temp.:30˚C

Sample MwRecovery

(%)

Lys-Bradykinin

Bradykinin

Met-Enkephalin

Neurotensin

Leu-Enkephalin

Substance P

Bacitracin

Insulin

Insulin B chain

Lysozyme

Mastoparan

Myoglobin

1

2

3

4

5

6

7

8

9

10

11

12

1188

1060

574

1673

556

1348

1450

5750

3496

14300

1479

17500

97

92

97

99

100

93

81

95

91

96

96

83

Sta

ndard

analysis

colu

mns

Polym

er-b

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d P

acked C

olu

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Ph

ase

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rom

ato

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hip

ak

2. 3-Methylhistidine

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

Polymer-based Packed Columns for Reversed Phase Chromatography : RSpak and Others

Features

F7622001

F7622002

F7622003

F6714010

F7622004

F7622005

F6714011

F6714012

F8700020

F7009000

F7009002

F7009001

F6709558

F7001001

F7001012

F6700140

F7001004

F7001005

F7009030

F7001006

F6700150

F7001007

F6700151

F7001002

F6700160

F7008140

F7008150

F6700510

F7008160

F7008240

F7008220

ODP2 HP-4B

ODP2 HP-4D

ODP2 HP-4E

ODP2 HPG-4A

ODP2 HP-2B

ODP2 HP-2D

ODP2 HPG-2A

ODP2 HP-4GC

HLD-4GC

RSpak RP18-415

RSpak RP18-613

RSpak RP18-413

RSpak RP18-G

RSpak DS-613

RSpak DS-413

RSpak DS-G

RSpak DE-613

RSpak DE-413

RSpak DE-413L

RSpak DE-413S

RSpak DE-G

RSpak DE-213

RSpak DE-SG

RSpak DM-614

RSpak DM-G

RSpak NN-814

RSpak NN-614

RSpak NN-G

RSpak NN-414

RSpak JJ-50 4D

RSpak JJ-50 2D

5

5

5

5

5

5

5

5

—

6

3.5

3.5

6

6

4

10

6

4

4

4

10

4

6

10

12

10

10

10

10

5

5

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O

—

H2O/CH3CN=5/95

H2O/CH3CN/THF=40/30/30






H2O

H2O/CH3CN=50/50

H2O/CH3CN=50/50

H2O/CH3CN=50/50

H2O

H2O/CH3CN=50/50

H2O/CH3CN=50/50

5mM H3PO4 aq.

5mM H3PO4 aq.

0.1M Sodium phosphate buffer(pH3.0)




H2O/CH3CN=40/60

H2O/CH3CN=40/60

Column Size(mm)

I.D. x L

4.6 x 50

4.6 x 150

4.6 x 250

4.6 x 10

2.0 x 50

2.0 x 150

2.0 x 10

4.0 x 10

—

4.6 x 150

6.0 x 150

4.6 x 150

4.6 x 10

6.0 x 150

4.6 x 150

4.6 x 10

6.0 x 150

4.6 x 150

4.6 x 250

4.6 x 50

4.6 x 10

2.0 x 150

2.0 x 10

6.0 x 150

4.6 x 10

8.0 x 250

6.0 x 150

6.0 x 50

4.6 x 150

4.6 x 150

2.0 x 150

Plate Number(TP/column)Product Name

ProductCode

ParticleSize(μm)

40

40

40

—

40

40

—

—

—

450

200

200

—

200

200

—

25

25

25

25

—

25

—

200

—

200

200

—

200

100

100

PoreSize(Å)

≧ 3,500

≧ 13,000

≧ 17,000

(guard column)

≧ 3,000

≧ 7,000

(guard column)

(cartridge guard)

(holder)

≧ 5,000

≧ 13,000

≧ 11,000

(guard column)

≧ 6,500

≧ 11,000

(guard column)

≧ 7,000

≧ 11,000

≧ 17,000

≧ 3,000

(guard column)

≧ 8,000

(guard column)

≧ 4,500

(guard column)

≧ 9,000

≧ 4,000

(guard column)

≧ 6,000

≧ 4,500

≧ 3,500

* See page 64 for the semi-micro columns and micro columns for DE-413 and NN-414.* See page 76 for the preparative columns for DE-613 and DM-614.* Contact Shodex or our distributors near you for custom columns.

pH range : RP18 1~13 DS, DE, NN 2~12 ODP2 3~12 DM, JJ 2~10

See also page 6 "Characteristics and Applications of Packing Materials for Normal & Reversed Phase Columns"

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

—

Sulfo

Sulfo

Sulfo

Sulfo

FunctionalGroup Base Material Shipping Solvent

• Improved theoretical plate number compared with general polymer-based columns, almost twice as good• Retain both polar and non-polar small molecules• No protein retention — can analyze small molecules in the presence of proteins• Ideal for LC/MS analysis — retain sharp peak shape even in low salt concentration

• For reversed phase analysis of proteins and peptides — large pore size

• For reversed phase analysis of highly hydrophilic substances, which cannot be retained well by ODS columns

• High-performance (high-theoretical-plate-number) version of DS-613 and DS-413

• Widest application because of similar polarity compared with ODS columns• Wide pH range enabling use in 100% water

• For the analysis of amino acids and water-soluble vitamins

• The packing material contains a trace amount of sulfo groups, it supports multi mode (reversed phase and cation exchange) analysis• Ideal for complex samples containing neutral and ionic substances

• The packing material contains a trace amount of quaternary ammonium groups, it supports multi mode (reversed phase and anion exchange) analysis• Ideal for complex samples containing neutral and ionic substances

ODP2 HP

RP18-415

DS-613, 413

RP18-613, 413

DE

DM-614

NN

JJ-50









—








Polymethacrylate

Polymethacrylate

Polymethacrylate

Polymethacrylate

Polymethacrylate

Polymethacrylate

Polymethacrylate







Polyvinyl alcohol

Polyvinyl alcohol

8

Polym

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Ph

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pak a

nd

Oth

ers

Sta

ndard

analysis

colu

mns

Notebook No.6 News No.30, 36

News No.14, 23, 24

News No.7

News No.7

Standard Columns

QuaternaryammoniumQuaternaryammonium

0 10 20 30 min min

2

13

4

5

6

0 5 10 15 20 25

1

45

6

7

78

2 3

0

1

2

5

34

5

6

10

7

8

15 20 min

ODP2 HP ODS

BarbitalPeak Area : 100%In

tens

ity

100002000030000400005000060000700008000090000

100000110000120000

0

Inte

nsity

100002000030000400005000060000700008000090000

100000110000120000

0

Inte

nsity

100002000030000400005000060000700008000090000

100000110000

0

Inte

nsity

100002000030000400005000060000700008000090000

100000110000

0

BarbitalPeak Area : 99%



0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5

Time (min)

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5

Time (min)

ODP2 HP-4D ODP-50 4D (existing)

112

2

3

34

4

N:11,400

N:5,700

0 5 10min

0 5 10min

0 20 40 60 80

5

4

1

2

3

32

1

6

0 5 10 15 minmin

12

3

4

5

6

0 2 4 6 8 10 min

Sample : 10μL1. Saccharin sodium 0.005%2. p-Hydroxybenzoic acid0.005%3. Sorbic acid 0.02%4. Benzoic acid 0.02%5. Methyl paraben 0.01%6. Dehydroacetic acid 0.01%7. Ethyl paraben 0.02%8. Propyl paraben 0.02%

Food additives (preservative)

Column :Shodex RSpak DE-413 Eluent : (50mM KH2PO4 + 0.1% H3PO4) aq. /CH3CN=65/35Flow rate :1.0mL/minDetector :UV(210nm)Column temp.:40˚C

1. Ribonuclease A

2. Insulin

3. Cytochrome c

4. Lysozyme

5. BSA

6. Myoglobin

7. Ovalbumin

93

98

100

100

98

108

－

Sample : 15μL1. Ethylene glycol 0.2%2. Methyl alcohol 1.0%3. Ethyl alcohol 0.4%4. Isopropyl alcohol 0.4%5. n-Propyl alcohol 0.4%6. tert-Butyl alcohol 0.4%7. sec-Butyl alcohol 0.4%8. n-Butyl alcohol 0.4%

Separation and recovery rate of standard protein by reversed phase Alkylalcohols

Column :Shodex RSpak RP18-415Eluent : (A); 0.1% TFA aq./CH3CN=99/1 (B); 0.1% TFA aq./CH3CN=5/95 Linear gradient; 20%(B) to 60%(B), 20min Flow rate :1.0mL/minDetector :UV(220nm)Column temp.:Room temp.

Column :Shodex RSpak DE-613Eluent :H2OFlow rate :1.0mL/minDetector :RIColumn temp.:60˚C

Barbital 500ng/mL

Barbital 500ng/mL in BSA 7.0mg/mL Barbital 500ng/mL in BSA 7.0mg/mL

Barbital 500ng/mL

Column :Shodex ODP2 HP-2B, ODSEluent :10mM CH3COONH4 aq./CH3CN=70/30Flow rate :0.2mL/minDetector :ESI-MS (SIM Negative : m/z 183)Column temp.:30˚CInjection vol. :10μL

Comparison of Recovery rate of Barbital in BSA between ODP2 HP-2B and ODS

Sample : 5μL1. Phenol 300mg/L2. Methyl benzoate 350mg/L3. Toluene 1000mg/L4. Naphthalene 150mg/L

Column :Shodex ODP2 HP-4D Eluent :H2O/CH3CN=55/45Flow rate :0.6mL/minDetector :UV(254nm)Column temp.:40˚C

Column :Shodex Asahipak ODP-50 4D Eluent :H2O/CH3CN=35/65Flow rate :0.6mL/minDetector :UV(254nm)Column temp.:40˚C

Comparison between ODP2 HP and Another Existing Column

Sample : 100mg/L each, 20μL1. p-Phenylenediamine2. m-Phenylenediamine3. o-Phenylenediamine

Sample : 20μL1. Aspartic acid2. Glycine3. Alanine4. Valine5. Methionine6. Isoleucine

Amino acid analysis using multimode Phenylenediamine Isomers analysis using multimode

Column :Shodex RSpak NN-814 Eluent :40mM H3PO4 aq.Flow rate :1.0mL/min Detector :RI Column temp.:40˚C

Column :Shodex RSpak JJ-50 4D Eluent :25mM Ammonium acetate buffer (pH9.2)/CH3CN=70/30 Flow rate :0.4mL/min Detector :UV(254nm) Column temp.:30˚C

Sample : 5μL1. m-Cresol 0.1%2. 2,4-Xylenol 0.1%3. Benzene 0.5%4. Toluene 0.5%5. Ethylbenzene 0.5%6. n-Propylbenzene 0.5%

Alkylbenzenes

Column :Shodex RSpak DS-613 Eluent :H2O/CH3CN/THF=30/40/30Flow rate :1.0mL/minDetector :UV(254nm)Column temp.:40˚C

9

Sta

ndard

analysis

colu

mns

For the LC/MS analysis of chemical substances containing proteins or other similar samples, it is generally said that an ODS column is susceptible to ion suppression due to the presence of proteins. However, ODP2 HP does not hold proteins and elutes them at one time, and thus is resistant to the effects of such ion suppression.

Polym

er-b

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Ph

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Ch

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ato

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pak a

nd

Oth

ers

Recovery of proteins (%)

F7630002

F7630001

F6710016

F7630006

F6713000

F6710100

F7001003

F6700170

Asahipak NH2P-50 4D

Asahipak NH2P-50 4E

Asahipak NH2P-50G 4A

Asahipak NH2P-50 2D


Asahipak NH2P-50 LF

RSpak DC-613

RSpak DC-G

10

Polymer-based Packed Columns for Normal Phase Chromatography

Features

• For the normal phase analysis of saccharides

• Polymer-based packing material provides excellent chemical stability and resistance to time degradation

• Washable with a basic solvent

• Wide pH range (pH2~13)

• For the normal phase analysis of saccharides

• Wide pH range (pH2~14)

NH2P-50

DC-613

Notebook No.2 News No.6, 14, 17

See also page 6, "Characteristics and Applications of Packing Materials for Normal & Reversed Phase Columns"

5

5

5

5

5

--—

6

10

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=30/70

H2O/CH3CN=30/70

Column Size(mm)

I.D. x L

4.6 x 150

4.6 x 250

4.6 x 10

2.0 x 150

2.0 x 10

8.0 x 75

6.0 x 150

4.6 x 10


Product NameProduct

Code

ParticleSize(μm)

100

100

—

100

—

—

100

—

PoreSize(Å)

≧ 5,500

≧ 7,500

(guard column)

≧ 3,500

(guard column)

(line filter)

≧ 5,500

(guard column)

* See page 72 for the semi-micro columns and micro columns for NH2P-50.* See page 85 for the preparative columns for NH2P-50 and DC-613.* Contact Shodex or our distributors near you for custom columns.

Amino

Amino

Amino

Amino

Amino

Amino

Sulfo(Na+)

Sulfo(Na+)

FunctionalGroup

Base Material Shipping Solvent

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol



Standard Columns

Polym

er-b

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d P

acked C

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r Norm

al P

hase

Ch

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ato

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ph

yS

tandard

analysis

colu

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NH2P-50 2D (2.0mm I.D. x 150mm) NH2P-50 4D (4.6mm I.D. x 150mm)

0 5 10 min

20 200 400

2

200000200000

400000

600000

1500

1000

500

0

12

14

10

8

0 10

1. Fructose2. Glucose3. Sucrose4. Maltose

1. Fructose2. Glucose3. Sucrose4. Maltose

0 10

16

20

18

0 50 100 150 2000.5 1

1

12

3

4

5

5

6

1

2

3

44

5

1

2

3

4

5

6

6

1

2 3

45

67

89

10

78

1

2

3

4

5

1

2

3

4

0 5 1510min

0 5 10 min 0 5 10 min

Erythorbic acidR2=0.9999

R2=0.9997L-Ascorbic acid

Erythorbic acid

12

14

10

8

16

20

18Maltose

Sucrose

Glucose

50 100 150 200

Fructose

Maltose

Sucrose

GlucoseFructose

L-Ascorbic acid

20 min 20 min

20

0 5 1510 20 min 0 5 10

0 10 20 min

4

3

After 180hrs

2

1

4

32

1

4 4

32

1

0 10 20 min

32

1

First use After 120hrsFirst use

NH2P-50 4E Silica-based amino column

15 min 0 10 20 30 min

Ve

(mL)

Time (hr) Time (hr)

Ve

(mL)

Pea

k he

ight

(μV

)P

eak

heig

ht(μ

V)

Sample concentration(μg/mL)

Sample concentration(μg/mL)

11

Ascorbic acid and Erythorbic acid Reproducibility with NH2P-50 column and silica-based amino column

Standard saccharides High sensitivity analysis of saccharides with semi-micro column

Sample : Oligogalacturonic acids1. Galacturonic acid2. Oligogalacturonic acid dimer3. Oligogalacturonic acid trimer4. Oligogalacturonic acid tetramer5. Oligogalacturonic acid pentamer6. Oligogalacturonic acid hexamer

Sample : Chitooligosaccharides 2%, 20μL1. D-Glucosamine2. Chitobiose hydrochloride3. Chitotriose hydrochloride4. Chitotetraose hydrochloride5. Chitopentaose hydrochloride6. Chitohexaose hydrochloride

Sample : 2.5% each, 15μL 1. Xylose 2. Fructose 3. Glucose 4. Galactose 5. Sucrose 6. Maltose 7. Lactose 8. Melibiose 9. Maltotriose10. Raffinose

Sample : 0.1% each, 20μL1. Fructose2. Sorbose3. Galactose4. Glucose5. Xylobiose6. Sucrose7. Lactose8. Maltose

Sample : 1. Fructose2. Glucose3. Sucrose4. Lactose5. MaltoseFructose: 8ng

Maltose :18ng

10mg/mL each, 2μL

Sample : 5μg/mL each, 10μg

1. Erythorbic acid

Oligogalacturonic acids Chitooligosaccharides Saccharides

Column :Shodex Asahipak NH2P-50 4E Eluent :0.3M Sodium phosphate buffer(pH4.4)Flow rate :1.0mL/minDetector :UV(210nm)Column temp.:40˚C

Column :Shodex Asahipak NH2P-50 4E Eluent :H2O/CH3CN=30/70Flow rate :1.0mL/min Detector :RI Column temp.:25˚C

Columnn :Shodex RSpak DC-613Eluent :H2O/CH3CN=20/80Flow rate :1.5mL/minDetector :RI Column temp.:70˚C

Column :Shodex Asahipak NH2P-50 4E�Eluent :20mM NaH2PO4 + 30mM H3PO4(pH2.2)/CH3CN=20/80Flow rate :1.0mL/minDetector :UV(254nm)Column temp.:30˚C

Column :Shodex Asahipak NH2P-50 4E Eluent :H2O/CH3CN=25/75Flow rate :1.0mL/minDetector :RIColumn temp.:30˚C

Column :Shodex Asahipak NH2P-50 2DEluent :H2O/CH3CN=25/75Flow rate :0.2mL/minDetector :RI(small cell volume) Column temp.:25˚C

Column :Shodex Asahipak NH2P-50 4DEluent :H2O/CH3CN=25/75Flow rate :1.0mL/min Detector :RI(conventional type)Column temp.:25˚C

Column :Shodex Asahipak NH2P-50 4E, Silica-based amino column Eluent :H2O/CH3CN=25/75Flow rate :1.0mL/minDetector :RIColumn temp.:30˚C

O=C

OHOC

HCHCOH

CH2OH

HOC

2. L-Ascorbic acidO=C

OHOC

HCHOCH

CH2OH

HOC

Detection limit (S/N=3)

Fructose:270ngMaltose :530ng

10.58mg/mL each, 10μL

Detection limit (S/N=3)

Sample offered by Prof. Yoshino in Kyoto Women's Univ.

For every kind of saccharide, the resistance to time degradation of our polymer-based amino column, NH2P-50, enables maintaining a stable retention time and obtaining sharp peaks.

Sta

ndard

analysis

colu

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Polym

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[ODS]

12

Silica-based Packed Columns for Normal and Reversed Phase Chromatography

Features

• Proven ODS column with a history of over 30 years

• Monomeric type ODS column realizing perfect end-capping with high purity silica (99.99% or higher)

• Polymereric type ODS column realizing perfect end-capping with high purity silica (99.99% or higher)• Excellent acid-resistance• Advantageous in separating planar and nonplanar compounds from each other

• For validation purposes (a set of three C18 4D columns with different lots of packing material)

F series

C18M

C18P

C18M 4D/V


F6604112

F6605110

F6604113

F6650040

F6650041

F6650042

F6650045

F6650046

F6650047

F6650064

ODSpak F-411

ODSpak F-511

ODSpak F-411/S

Silica C18M 4D

Silica C18M 4E

Silica C18M 2D

Silica C18P 4D

Silica C18P 4E

Silica C18P 2D

Silica C18M 4D/V

5

5

3

5

5

5

5

5

5

5

H2O/CH3OH=20/80

H2O/CH3OH=20/80

H2O/CH3OH=20/80

H2O/CH3OH=30/70

H2O/CH3OH=30/70

H2O/CH3OH=40/60

H2O/CH3OH=30/70

H2O/CH3OH=30/70

H2O/CH3OH=40/60

H2O/CH3OH=30/70

Column Size (mm)I.D. x L

4.6 x 150

4.6 x 250

4.6 x 100

4.6 x 150

4.6 x 250

2.0 x 150

4.6 x 150

4.6 x 250

2.0 x 150

4.6 x 150, 3pc


ProductCode

Particle Size(μm)

14

14

14

16

16

16

17

17

17

16

Carbon Load(%)

100

100

100

100

100

100

100

100

100

100

Pore Size(Å)

≧ 8,000

≧ 14,000

≧ 10,000

≧ 10,000

≧ 16,000

≧ 9,000

≧ 10,000

≧ 16,000

≧ 9,000

≧ 10,000

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

Octadecyl

FunctionalGroup

Shipping Solvent

Standard Columns

* See page 77 for the preparative columns for C18M.* Contact Shodex or our distributors near you for custom columns.

Base Material : SilicaSilica C18M 4D/V is a set of three columns.

[Other Silica type columns]

Features

• Silica-based column without any chemical modification

• High purity silica (99.99% or higher)• Like E-411, suitable for normal phase analysis using a nonpolar, organic solvent

• Retention capacity is lower than that of C18 but higher than that of 5C4 or 5TMS• Applicable to ion pair chromatography because of its high permeability and short time to achieve equilibrium

• Retention capacity is lower than that of C18 or C8

• Utilizes reversed phase interaction and p-p interaction to separate regioisomers, that typically cannot be separated with ODS, C8, or C4 columns

• Utilizes several kinds of interactions based on π-electrons to separate structural isomers

• For normal phase analysis of saccharides

E-411

5SIL

5C8

5C4 · 5TMS

5CN

5NPE · 5PYE

5NH


F6604110

F6650050

F6650051

F6650052

F6650053

F6650054

F6650055

F6650056

F6650057

F6650058

F6650059

F6650062

F6650063

F6650060

F6650061

Silicapak E-411

Silica 5SIL 4D

Silica 5SIL 4E

Silica 5C8 4D

Silica 5C8 4E

Silica 5C4 4D

Silica 5C4 4E

Silica 5TMS 4D

Silica 5TMS 4E

Silica 5CN 4D

Silica 5CN 4E

Silica 5NPE 4D

Silica 5PYE 4D

Silica 5NH 4D

Silica 5NH 4E

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

C6H14/C2H5OH=95/5

C6H14/C2H5OH=95/5

H2O/CH3OH=34/66

H2O/CH3OH=34/66

H2O/CH3OH=45/55

H2O/CH3OH=45/55

H2O/CH3OH=50/50

H2O/CH3OH=50/50

H2O/CH3OH=60/40

H2O/CH3OH=60/40

H2O/CH3OH=45/55

H2O/CH3OH=30/70

H2O/CH3CN=5/95

H2O/CH3CN=5/95


4.6 x 150

4.6 x 150

4.6 x 250

4.6 x 150

4.6 x 250

4.6 x 150

4.6 x 250

4.6 x 150

4.6 x 250

4.6 x 150

4.6 x 250

4.6 x 150

4.6 x 150

4.6 x 150

4.6 x 250


ProductCode

Particle Size(μm)

—

—

—

10

10

7

7

—

—

—

—

—

—

—

—

Carbon Load(%)

50

100

100

100

100

100

100

100

100

100

100

100

100

100

100

Pore Size(Å)

≧ 8,000

≧ 9,000

≧ 15,000

≧ 9,000

≧ 15,000

≧ 9,000

≧ 15,000

≧ 7,000

≧ 12,000

≧ 7,000

≧ 12,000

≧ 8,000

≧ 7,000

≧ 5,000

≧ 8,000

—

—

—

Octyl

Octyl

Butyl

Butyl

Trimethylsilyl

Trimethylsilyl

Cyanopropyl

Cyanopropyl

Nitrophenylethyl

Pyrenylethyl

Aminopropyl

Aminopropyl

FunctionalGroup

Shipping Solvent

Standard Columns

* See page 77 for the preparative columns for 5SIL, 5C8 and 5C4.* Contact Shodex or our distributors near you for custom columns.

Base Material : Silica

IPA/Dichloromethane/n-Hexane=1/50/49

Silic

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2 6

10 min255 15 2010

0 10

10 20

20 30 min

8

9

10

11

0 30 40 min

5010 min5 min

min min

Catechins in green tea

Aflatoxins

Column :Shodex Silica C18P 4D Eluent :0.1% H3PO4 aq./CH3OH=20/80Flow rate :1.0mL/minDetector :UV(254nm) Column temp.:30˚C

Sample : 1. Daidzin 1.0μg2. Genistin 0.5μg3. Daidzein 1.0μg4. Genistein 1.0μg

Sample : 1. Tartrazine 1.0μg 2. Amaranth 1.0μg 3. Indigo carmine 1.0μg 4. New coccine 1.0μg 5. Sunset yellow FCF 1.0μg 6. Fast green FCF 3.0μg 7. Brilliant blue FCF 3.0μg 8. Acid red 1.0μg 9. Erythrosine 1.0μg10. Phloxine 1.0μg11. Rose bengale 1.0μg

Sample : 1. (-)-Epigallocatechin 1.0μg2. (-)-Catechin 1.0μg3 (-)-Epigallocatechin gallate 0.5μg 4. (-)-Catechin gallate 1.0μg

Isoflavones Artificial colorants

Column :Shodex Silica C18P 4D Eluent :0.1% Acetic acid aq./CH3OH=70/30Flow rate :1.0mL/minDetector :UV(254nm) Column temp.:30˚C

Column :Shodex Silica C18P 4D Eluent : (A); 20mM Phosphate buffer(pH7.0) /CH3OH=90/10 (B); 20mM Phosphate buffer(pH7.0) /CH3OH=20/80 Linear gradient; 15%(B) to 100%(B)Flow rate :1.0mL/minDetector :UV(254nm) Column temp.:30˚C

Column :Shodex Silica C18M 4E Eluent :H2O/CH3OH=60/40Flow rate :0.8mL/minDetector :Fluorescence detector (Ex.365nm, Em.450nm)Column temp.:40˚C

Sample : 10μL1. Mandelic acid 17μg/mL2. Hippuric acid 17μg/mL3. o-Methylhippuric acid 17μg/mL4. p-Methylhippuric acid 17μg/mL5. m-Methylhippuric acid 17μg/mL6. Creatinine 33μg/mL

Sample : 20μL1. Derived Aflatoxin G12. Derived Aflatoxin B13. Aflatoxin G24. Aflatoxin B2

Column :Shodex ODSpak F-411 Eluent :〔20mM H3PO4 + 20mM β-Cyclodextin(pH2.5)〕/CH3CN=88/12 + 4.5mM Sodium dodecyl sulfateFlow rate :1.0mL/minDetector :UV(225nm)Column temp.:50˚C

Hippuric acids in urine

Sample : 1. Naphtalene2. 1,5-Dinitronaphtalene3. 1,5-Dinitronaphtalene4. 1,8-Dinitronaphtalene

Sample : 1. 2-Benzylpyridine2. 3-Benzylpyridine3. 4-Benzylpyridine

Benzylpyridine isomers Dinitronaphthalene isomers

Column :Shodex Silica 5PYE 4D Eluent :20mM KH2PO4 aq./CH3OH=40/60Flow rate :1.0mL/minDetector :UV(254nm) Column temp.:30˚C

Column :Shodex Silica 5NPE 4D Eluent :H2O/CH3OH=30/70Flow rate :1.0mL/minDetector :UV(254nm) Column temp.:30˚C

Sample : R1 R2

1. α-Tocopherol -CH3 -CH3

2. β-Tocopherol -CH3 -H3. γ-Tocopherol -H -CH3

4. δ-Tocopherol -H -H

Vitamin E

Column :Shodex Silica 5SIL 4D Eluent :n-Hexane/Isopropanol=99.5/0.5Flow rate :1.0mL/minDetector :UV(280nm) Column temp.:30˚C

R1

R2

HO

O

CH3CH3

CH3 CH3 CH3

CH3

13

Standard Urine(10-fold diluted)

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F6118250

F6113000

IEC SP-825

IEC SP-420N

Strong cation exchange resin ( Functional Group : Sulfopropyl )

14

Columns for Cation Exchange Chromatography

All Shodex IEC columns are packed with chemically stable polymers that can be used in pH2 to 12.

Features

• For the analysis of high-molecular-weight molecules, such as proteins, peptides, DNA and RNA

• Polyhydroxy methacrylate-based packing material supports the use in pH range of 2 to 12

• Semi-micro, micro and preparative columns are available

• Nonporous base material

• Rapid analysis

• Wide pH range (pH2 to 12)

• Polyvinyl alcohol-based packing material supports the use of eluent in a wide pH range (2 to 12)

• Less hydrophobic interaction with proteins allows milder analytical conditions

• Semi-micro, micro and preparative columns are available

SP-825, CM-825

SP-420N

ES-502C 7C

Standard Columns

0.4

0.3

8

2.5

5,000

—

Column Size(mm)

I.D. x L

8.0 x 75

4.6 x 35

Product NameProductCode

ParticleSize(μm)

Ion ExchangeCapacity(meq/g)

PoreSize(Å)




50mM Na2SO4 aq.

20mM Sodium acetate buffer

+ 0.5M Na2SO4 (pH5.0)

* See page 65 for the semi-micro columns and micro columns.* See page 78 for the preparative columns for SP-825.* Contact Shodex or our distributors near you for custom columns.

F6110002

F7640001

IEC CM-825

Asahipak ES-502C 7C

Week cation exchange resin ( Functional Group : Carboxymethyl )

0.4

0.55

8

9

5,000

2,000

Column Size(mm)

I.D. x L

8.0 x 75

7.5 x 100


ParticleSize(μm)


PoreSize(Å)



Polyvinyl alcohol

50mM Na2SO4 aq.

0.1M Sodium phosphate buffer

(pH4.4)

* See page 65 for the semi-micro columns and micro columns.* See page 78 for the preparative columns for CM-825 and ES-502C 7C.* Contact Shodex or our distributors near you for custom columns.

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11

1

2

2

2

3

3

3

33

31 1

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AS

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4 4 44

5

5 5

5

5

3

3

4

4

4

5

5

5

6 6

6

0 010 30 min 15 min 0 15 min 0 15 min 0

0 3 6 9 min

15 min

pH4.0 pH5.0 pH6.0 pH7.0 pH8.0

0 0 0 10 20 min10 20 30 min

1

2

3

4

1

2

3

20 min

CH2·CH·NH2

COOH

HOHO

CH·CH2·NH

CH3OH

OH

HOHO

CH·CH2·NH2HOHO

CH2·CH2·NH2HOHO

N NH3C CH3 2Cl-

2Br-

+ +

N++N

15

Effect of eluent pH using SP-825 Protein separation using SP-420N (nonporous type gel)

Protein separation using cation exchange column

Column : (Ⅰ) Shodex IEC CM-825 (Ⅱ) Shodex IEC SP-825 (Ⅲ) Shodex IEC SP-420N Eluent : (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl (Ⅰ,Ⅱ) Linear gradient; (A) to (B), 60min (Ⅲ) Linear gradient; (A) to (B), 10minFlow rate : (Ⅰ,Ⅱ) 1.0mL/min (Ⅲ) 1.5mL/minDetector :UV(280nm)Column temp.:Room temp.

Column : Shodex IEC SP-825 Eluent : (A); 20mM Sodium formate buffer(pH4.0) 20mM Sodium malonate buffer(pH5.0 and 6.0) 20mM Sodium phosphate buffer(pH7.0) 20mM HEPES(pH8.0) (B); (A) + 0.5M NaCl Linear gradient; (A) to (B), 20minFlow rate: 1.0mL/min

Column :Shodex IEC SP-420N Eluent : (A); 20mM MES* buffer(pH5.6) (B); (A) + 0.5M Na2SO4 Linear gradient; 5%(B) to 100%(B), 30minFlow rate :1.5mL/minDetector :VIS(415nm)Column temp.:Room temp.*MES: 2-(N-Morpholino)ethanesulfonic acid

Sample : 300μg/mL each, 10μL Sample : 10μL1. Angiotensin Ⅱ(human) Asp-Arg-Val-Tyr-lle-His-Pro-Phe

2. AngiotensinⅠ(human) Asp-Arg-Val-Tyr-lle-His-Pro-Phe- His-Leu

3. AngiotensinⅢ(human) Arg-Val-Tyr-lle-His-Pro-Phe

Sample : 20μLParaquat

Diquat

Sample :1. Myoglobin2. Trypsinogen 3. Ribonucrease A4. α-Chymotrypsinogen A 5. Cytochrome c 6. Lysozyme

Sample : 30μL1. Myoglobin 0.03%2. Ribonuclease A 0.17%3. α- Chymotrypsinogen A 0.08%4. Cytochrome c 0.08%5. Lysozyme 0.08%

Sample : 0.5% each, 2μL Hemoglobin A,F,S,C

Catecholamines Angiotensins Paraquat and Diquat

Column :Shodex Asahipak ES-502C 7CEluent :20mM Sodium malonate buffer + 0.5M NaCl(pH6.0) Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:30˚C

Column :Shodex Asahipak ES-502C 7CEluent :50mM Sodium maronate buffer(pH6.0) /CH3CN=80/20Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:30˚C

Column :Shodex Asahipak ES-502C 7CEluent :50mM Sodium phosphate buffer + 150mM NaCl(pH7.0) Flow rate :1.0mL/minDetector :UV(288nm)Column temp.:30˚C

（Ⅰ）CM-825 (weak cation ion exchange)

90μL injected

（Ⅱ）SP-825 (strong cation ion exchange)

30μL injected

（Ⅲ）SP-420N (strong cation ion exchange)

nonporous type gel

1. DOPA

2. Adrenaline

3. Noradrenaline

4. Dopamine

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NEWNEW

NEWNEW

50mM 1,3-Diaminopropane + 50mM NaCl (pH10.0)

H2O

0.1M Sodium phosphate buffer(pH3.0)/CH3CN=80/20

0.1M Sodium phosphate buffer(pH3.0)/CH3CN=80/20

16

Columns for Anion Exchange Chromatography

Columns for Hydrophobic Interaction Chromatography

All Shodex IEC columns are packed with chemically stable polymers that can be used in wide pH range.

Features

• For the analysis of high-molecular-weight molecules, such as proteins, peptides, DNA and RNA• Polyhydroxymethacrylate-based packing material supports the use in pH range 2 to 12• Semi-micro, micro and preparative columns are available

• Nonporous base material (pH2 to 12)• Rapid analysis

• Polyvinyl alcohol-based packing material supports the use in pH range 2 to 12• Less hydrophobic interaction with proteins allows milder analytical conditions• Semi-micro, micro and preparative columns are available

• For anion exchange analysis of small molecules such as nucleotides (pH3~12)

QA-825, DEAE-825

DEAE3N-4T

ES-502N 7C

WA-624

PH-814

Features

• Separates proteins without denaturation

• Applicable to sample after treatment of ammonium sulfate fractions (pH2~12)

F6110011 IEC QA-825

Strong anion exchange resin ( Functional Group : Sulfopropyl )

Standard Columns

Standard Columns

0.45 12 5,000

Column Size(mm)

I.D. x L

8.0 x 75

Product NameProduct

Code

ParticleSize(μm)


PoreSize(Å)


Polyhydroxymethacrylate 50mM Na2SO4 aq.

* See page 66 for the semi-micro columns and micro columns.* See page 78 for the preparative columns.* Contact Shodex or our distributors near you for custom columns.

F6110003 HIC PH-814 Phenyl 10 2,000

Column Size(mm)

I.D. x L

8.0 x 75

Product NameProduct

CodeFunctional

Group

ParticleSize(μm)

PoreSize(Å)


Polyhydroxymethacrylate H2O

* Contact Shodex or our distributors near you for custom columns.

F6118255

F6112100

F7640002

F6356240

F6700245

IEC DEAE-825

IEC DEAE3N-4T

Asahipak ES-502N 7C

AXpak WA-624

AXpak WA-G

Weak anion exchange resin ( Functional Group : Diethylaminoethyl )

0.6

0.4

0.55

1.2

(guard column)

8

2.5

9

10

10

5,000

—

2,000

2,000

—

Column Size(mm)

I.D. x L

8.0 x 75

4.6 x 35

7.5 x 100

6.0 x 150

4.6 x 10

Product NameProduct

Code

ParticleSize(μm)


PoreSize(Å)




Polyvinyl alcohol



50mM Na2SO4 aq.

* See page 66 for the semi-micro columns and micro columns for DEAE-825, DEAE3N-4T and ES-502N 7C.* See page 78 for the preparative columns for DEAE-825 and ES-502N.* Contact Shodex or our distributors near you for custom columns.

News No.33

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0

0 0 10 15 20 0 20 400 20 40 60 min

60 min25 min

0 10 20 min

15 30 min

0 5 10 15 min

0 5 10 15 min 0 5 10 15 min 0 5 10 15 min

0 5 10 15 min

1

1

pH6.0

pH8.0

pH7.0

pH9.0 pH10.0

2 2

43

1

24

3

1

2

43

1,2

4

3

43

CH3

HO

HOOC COOH

CH3

HO

HOOC

1. endo GA14

2. GA14

COOH

1

1

2

34

5

1

2

3

6

2

3

4

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7

8 9

10

0 5 10 15 20 25 30 min

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0 5 min

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17

Protein separation using QA-825

Sample : 1. 5'CMP 2. 5'UMP 3. 5'TMP 4. 3'CMP 5. 5'ADP 6. 3'UMP 7. 5'GMP 8. 2'AMP 9. 3'AMP10. 3'GMP

Sample : 370μL1. Cytochrome c 0.03%2. Myoglobin 0.08%3. Ribonuclease A 0.16%4. Ovalbumin 0.16%5. Lysozyme 0.04%6. α Chymotrypsinogen 0.05%

Sample : Egg white 1%, 200μL1. Conalbumin2. Ovalbumin3. Lysozyme

Sample : 100μL1.Conalbumin 0.02%2.Transferrin 0.17%3.Ovalbumin 0.14%4.Trypsin inhibitor 0.17%

Nucleotides Protein separation using hydrophobic interaction chromatography

Egg white protein separation using hydrophobic interaction chromatography

Column :Shodex AXpak WA-624Eluent :0.35M Ammonium acetate buffer (pH4.3)Flow rate :1.0mL/minDetector :UV(260nm)Column temp.:60˚C

Column :Shodex HIC PH-814 Eluent : (A); 1.8M Ammonium sulfate + (B) (B); 0.1M Phosphate buffer(pH7.0) Linear gradient; (A) to (B), 60minFlow rate :1.0mL/min Detector :UV(280nm) Column temp.:Room temp.

Column :Shodex HIC PH-814 Eluent : (A); 1.8M Ammonium sulfate + (B) (B); 0.1M Phosphate buffer(pH7.0) Linear gradient; (A) to (B), 60minFlow rate :0.5mL/min Detector :UV(280nm) Column temp.:Room temp.

Sample : Gibberellin

Comparison data between porous DEAE-825 and non-porous DEAE3N-4T Gibberellin Isomers

Column :Shodex IEC QA-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0) (B); (A) + 0.5M NaCl Linear gradient; 100%(A) to 50%(B), 30minFlow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Column : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0) (B); A + 0.5M NaCl Linear gradient; (A)→(B), 60minFlow rate : 1.0mL/min Detector : UV (280nm) Column temp.: Room temp.Injection vol. : 100μL

Effect of eluent pH using DEAE-825

Sample : 100μL1. Conalbumin 0.06% 2. Transferrin 0.27% 3. Ovalbumin 0.33% 4. Trypsin inhibitor 0.33%

Column :Shodex IEC DEAE-825 Eluent :(A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Tris-Trispropanol-HCl buffer(pH7.0) 20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0) 20mM 1,3-Diaminopropane-HCl buffer(pH10.0) (B); (A) + 0.5M NaCl Linear gradient; (A) to (B), 20minFlow rate :1.0mL/min

Column :Shodex Asahipak ES-502N 7C Eluent :Acetic acid/H2O/CH3OH =0.1/0.4/99.5Flow rate :1.5mL/minDetector :UV(210nm)Column temp.:50˚C

Data was provided by Prof. Yamaguch, Faculty of Agliculture, University of Tokyo.

Sample : 1. Conalbumin 0.4mg/mL2. Ovalbumin 1.2mg/mL3. Trypsin inhibitor 1.0mg/mL

Column : Shodex IEC DEAE3N-4TEluent : (A); 25mM Piperazine-HCl buffer(pH6.0) (B); A + 0.5M NaCl Linear gradient; (A)→(B),10minFlow rate : 1.5mL/min Detector : UV (280nm) Column temp.: Room temp.Injection vol. : 20μL

porous gel DEAE-825 nonporous gel DEAE3N-4T

: Diethylaminoethyl : Diethylaminoethyl

IEC DEAE3N-4T is a weak anion exchange column. Diethyaminoethyl group is introduced on the surface of a non-porous gel. Rapid analysis of proteins and peptides are possible with non-porous packing materials. Its peak shape is sharp, allowing analysis of a small sample size.

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NEWNEW

NEWNEW

NEWNEW

18

Columns for Ion Chromatography (Cation analysis)

Features

• Higher performance type of YK-421

• Applicable to analysis with or without suppressor method

• Improved peak shape especially of divalent cations

• Supports the analysis of alkylamines and transition metals

• Cation analysis with non-suppressor method

• Simultaneous analysis of monovalent and divalent cations

• For the analysis of alkyl amines

• Cation analysis with non-suppressor method

• For analysis of monovalent or divalent cations respectively

• T-521 and R-621 are applicable to the analysis of transition metal and rare-earth metal ions respectively

• High sensitivity achieved by using post-column fluorescence method

YS-50

YK-421

Y-521

T-521, R-621

Standard Columns

Line Filter for IC

F7122000

F6700530

F7120012

F6709608

F6995210

F6700230

IC YS-50

IC YS-G

IC YK-421

IC YK-G

IC Y-521

IC Y-G

For cation

≧ 5,500

(guard column)

≧ 2,800

(guard column)

≧ 3,000

(guard column)

5

5

5

5

12

12

Column Size(mm)

I.D. x L

4.6 x 125

4.6 x 10

4.6 x 125

4.6 x 10

4.6 x 150

4.6 x 10



ParticleSize(μm)

FunctionalGroup

Shipping Solvent

Carboxyl

Carboxyl

Carboxyl

Carboxyl

Sulfo

Sulfo

Base Material

Polyvinyl alcohol

Polyvinyl alcohol

Silica

Silica



4mM Methanesulfonic acid aq.

4mM Methanesulfonic acid aq.

4mM HNO3 aq.

4mM HNO3 aq.


5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.

5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.

F6995250

F6700412

IC T-521

IC T-G

For transition metal ion

≧ 3,000

(guard column)

12

12

Column Size(mm)

I.D. x L

4.6 x 150

4.6 x 10



ParticleSize(μm)

FunctionalGroup

Shipping Solvent

Sulfo

Sulfo

Base Material



3mM HNO3 aq.

3mM HNO3 aq.


F6998000

F6709090

F8500630

F8500640

F8500650

F8500660

F6700240

For general purposes (not for the substitution of Y-521L)

Replace filter for IC FL-1

non-metal type (not for the substitutionof Y-521L)


For the analysis of monovalent cations (used with Y-521)

one holder and one filter

5 filters


4 filters

—

IC R-621

IC R-G

IC FL-1

IC FL-1 filter

IC FL-2

IC FL-2 filter

IC Y-521L

For rare earth metal ion

≧ 1,000

(guard column)

5

5

Column Size(mm)

I.D. x L


6.0 x 50

4.6 x 10

—

—

—

—

8.0 x 50

Product Name

Product Name Application Contents

ProductCode

ProductCode


ParticleSize(μm)

FunctionalGroup

Shipping Solvent

Sulfo

Sulfo

Base Material



H2O

H2O


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Notebook No.4 News No.28

0 5 10 15 20 min

min

1

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3.0

5

5

1 2 3

4

1

2

2

1 2 3 4 5 6 7 8 9 10 min

3

4

1

Cu2+

Zn2+

Co2+

Mg2+

Mn2+Cd2+

Pb2+Ca2+

Sr 2+

Fe2+

Ni2+

2

3

4 5

1

2

3

4

5

6

6

2

0

4

6

7

5

6

6

6

4 5

4

4

5

2

3

4

56

1

2

4

3

5

6

YS-50 YK-421

0 5 10

1 2 3 4 5 6 7 8 9 10 min

15 20 min

0 5 10 15 20 min

0 1284 242016 28 min

min

20 864 10 min

0 5 10 15 min

5μ

S/c

m

5μ

S/c

m

6mM

3mM

1mM

0mM

18-C

row

n 6-

ethe

r co

nc.

3.5 4.0 4.5

19

Common cations (YS-50 and YK-421)

Sample : 1. Li+ 2mg/L2. Na+ 10mg/L3. NH4

+ 10mg/L4. K+ 20mg/L5. Mg2+ 10mg/L6. Ca2+ 20mg/L

Sample : 10μLSeawater (100 fold diluted)1. Na+ 98.0mg/L2. NH4

+ 0.4mg/L3. K+ 3.6mg/L4. Mg2+ 11.1mg/L5. Ca2+ 3.4mg/L

Sample : 50μL1. NH4

+ 5mg/L2. Methylamine 5mg/L3. Dimethylamine 5mg/L4. Trimethylamine 10mg/L5. Ethylamine 10mg/L6. Propylamine 10mg/L7. Butylamine 10mg/L

Sample : 100μL1. Mg2+ 20mg/L2. Ca2+ 50mg/L3. Sr2+ 100mg/L4. Ba2+ 200mg/L

Sample : 70μLRed wine (diluted 2-fold followed by addition of 40mL HCl) 1. Fe3+

2. Zn2+

3. Co2+

4. Fe2+

5. Mn2+

Sample : 10μL1. Li+ 2mg/L2. Na+ 10mg/L3. NH4

+ 10mg/L4. K+ 20mg/L5. Mg2+ 10mg/L6. Ca2+ 20mg/L

10μL inj. 15μL inj.

Alkylamines Alkaline earth metal ions Transition metal ions in red wine

Column :Shodex IC YK-421 Eluent :4mM H3PO4 aq. + 10% CH3CN Flow rate :1.0mL/minDetector :Non-suppressed conductivityColumn temp.:25˚C

Column :Shodex IC Y-521Eluent :4mM HNO3 aq.Flow rate :1.5mL/minDetector :Non-suppressed conductivityColumn temp.:20˚C

Column :Shodex IC T-521 Eluent :6mM Oxalic acid + 3mM Citric acid (pH4.1 adjusted by KOH) + 0.1mM NaN3 + 0.1mM NaHSO3Reagent :0.2mM *PAR + 3M NH4OH + 1M Acetic acid aq. [Mixing coil (0.5mm I.D. x 1m)]Flow rate :1.0mL/minDetector :VIS(530nm)Column temp.:40˚C

Column :Shodex IC YS-50Eluent :4mM Methanesulfonic acid aq.Flow rate :1.0mL/minDetector :Non-suppressed conductivityColumn temp.:40˚C

Column :Shodex IC YK-421Eluent :5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.Flow rate :1.0mL/minDetector :Non-suppressed conductivityColumn temp.:40˚C

100-fold dilution of sea water

Column :Shodex IC YS-50Eluent :4mM Methanesulfonic acid aq.Flow rate :1.0mL/minDetector :Non-suppressed conductivityColumn temp.:40˚C

Effect of adding crown ether to eluent

Column : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid + 18-Crown 6-ether aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp.: 40˚C

YS-50 is a high-performance column whose number of theoretical plates is approximately twice as large as that of YK-421. In particular, the peak shape and quantitative performance for divalent ions have been improved.The quantitative performance for NH4

+ in a system containing Na+ at a high concentration has been enhanced as well

A crown ether molecule forms complex with a cation. The elution of cations (in particular, K+) can be controlled utilizing the difference in the ability of complex formation

Resolution factor of Na+and NH4

+

YS-50

TP

YK-421

YS-50 YK-421

2.3

7,000 3,200

6,900 3,300

1.8

Mg2+

Ca2+

*PAR : 4-(2-Pyridylazo)resorcinol

Sta

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analysis

colu

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hro

mato

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phy (C

atio

n a

nalysis )

NEWNEW

NEWNEW

NEWNEW

20

Columns for Ion Chromatography (Anion analysis)

Features

• Anion analysis with non-suppressor method• NI-424 supports the simultaneous analysis of fluoride and phosphate ions

• Anion analysis with suppressor method• Suitable for the determination of fluoride ions• SI-50 separates the target substance from an organic acid well• Not affected by carbonate system peaks

• Anion analysis in wine with suppressor method

• For analysis of oxyhalides• SI-52 supports simultaneous analysis of halogen oxides and general inorganic ions• SI-91 is used with post-column method

NI-424, I-524A

SI-90, SI-50

VH-anion

SI-52, SI-91

News No.15

News No.5

News No.37

News No.34

Standard Columns

Line Filter for IC

F6995243

F6709616

F6995240

F6700400

IC NI-424

IC NI-G

IC I-524A

IC IA-G

For anion ( non-suppressor method )

≧ 5,000

(guard column)

≧ 2,000

(guard column)

5

5

12

12


4.6 x 100

4.6 x 10

4.6 x 100

4.6 x 10



Particle Size(μm)

Shipping SolventFunctional Group

Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

2.5mM Phthalic acid aq.

2.5mM Phthalic acid aq.

* Contact Shodex or our distributors near you for custom columns. Base Material : Polyhydroxymethacrylate

8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris + 2mM Phenylboronic acid + 0.005mM CyDTA aq.

8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris + 2mM Phenylboronic acid + 0.005mM CyDTA aq.

F6995244

F6995245

F6709620

F6995245

F6709620

IC SI-90 4E

IC SI-50 4E

IC SI-90G

IC VH-anion 4D

IC VH-anionG 4A

For anion ( suppressor method )

≧ 5,000

≧ 10,000

(guard column)

≧ 7,000

(guard column)

9

5

9

5

9


4.0 x 250

4.0 x 250

4.6 x 10

4.6 x 150

4.6 x 10



Particle Size(μm)


Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

1.8mM Na2CO3 + 1.7mM NaHCO3 aq.





F8500630

F8500640

F8500650

F8500660

IC FL-1

IC FL-1 filter

IC FL-2

IC FL-2 filter


ContentsApplication

For general purposes (not for the substitution of Y-521L)


non-metal type (not for the substitutionof Y-521L)



5 filters


4 filters

* Contact Shodex or our distributors near you for custom columns. Base Material : Polyvinyl alcoholHousing material : PEEK

F6995260

F6995280

F6709620

IC SI-52 4E

IC SI-91 4C

IC SI-90G

For oxyhalides

≧ 14,000

≧ 2,500

(guard column)

5

9

9


4.0 x 250

4.0 x 100

4.6 x 10



Particle Size(μm)


Quaternary ammonium

Quaternary ammonium

Quaternary ammonium

3.6mM Na2CO3 aq.



* Contact Shodex or our distributors near you for custom columns. Base Material : Polyvinyl alcoholHousing material : PEEK

Colu

mns fo

r Ion C

hro

mato

gra

phy (A

nio

n a

nalysis )

Sta

ndard

analysis

colu

mns

0 2 4 6 8 10 12 14 min 0 2 4 6 8 10 12 14 16 min

2 4 6 8 10 12 14

0

0 5 10 min

5 10 15 20 25 30 min

16 18 20 22 24 26 28 30 min0 10 min5

1

3

4

5

6

7

,2

1

3

45

6

7

2

1

34

5

6

6

7

8

2

1

3 4

5 78

9

10

11

2

6

1

1

3

3

4

4

5

7 8

9

10

11

2

2

I-524A NI-424

21

Anion analysis with non-suppressor method (NI-424 and I-524A)

Sample : 50μL1. H2PO4

- 20mg/L2. F- 　　�1.5mg/L3. CI- 2.5mg/L4. NO2

- 6mg/L5. Br- 10mg/L6. NO3

- 10mg/L7. SO4

2- 10mg/L

Sample : 20μL1. F- 2mg/L2. Cl- 3mg/L3. NO2

- 5mg/L4. Br- 10mg/L5. NO3

- 10mg/L6. HCO3

- 300mg/L7. PO4

3- 15mg/L8. SO4

2- 15mg/L

Sample : 20μL 1. F- 2mg/L 2. Acetic acid 10mg/L 3. Formic acid 2mg/L 4. Methacrylic acid 10mg/L 5. Cl- 3mg/L 6. NO2

- 5mg/L 7. Br- 10mg/L 8. NO3

- 10mg/L 9. PO4

3- 15mg/L10. SO4

2- 15mg/L11. Oxalic acid 15mg/L

Sample : 50μL 1. F- 2mg/L 2. ClO2

- 1mg/L 3. BrO3

- 1mg/L 4. Cl- 10mg/L 5. NO2

- 5mg/L 6. Br- 10mg/L 7. ClO3

- 1mg/L 8. Dichloroacetate 1mg/L 9. NO3

- 30mg/L10. PO4

3- 15mg/L11. SO4

2- 40mg/L

Sample : 20μL (50-hold dilution)1. Cl- 289mg/L2. PO4

3- 621mg/L3. SO3

2- 1840mg/L4. SO4

2- 815mg/L

Oxyhalides analysis with suppressor method using SI-52 4E

Column :Shodex IC SI-52 4EEluent :3.6mM Na2CO3 aq.Flow rate :0.8mL/minDetector :Suppressed conductivityColumn temp.:45˚C

Column :Shodex WINE VH-anionG 4A + Shodex WINE VH-anion 4DEluent :5mM Na2CO3 + 12mM NaHCO3 + 3% AcetoneFlow rate :0.8mL/minDetector :Suppressed conductivityColumn temp.:30˚C

Column :Shodex IC I-524A Eluent :2.5mM Phthalic acid + 2.3mM Tris(pH4.2)Flow rate :1.2mL/minDetector :Non-suppressed conductivityColumn temp.:40˚C

Column :Shodex IC NI-424 Eluent :8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris + 2mM Phenylboronic acid + 5μM *CyDTA(pH4.2)Flow rate :1.0mL/minDetector :Non-suppressed conductivityColumn temp.:40˚C

Anion analysis with suppressor method using SI-90 4E

Column : Shodex IC SI-90 4EEluent : 1.8mM Na2CO3 + 1.7mM NaHCO3 aq.Flow rate : 1.2mL/minDetector : Suppressed conductivityColumn temp.: 25˚C

Anion analysis with suppressor method using SI-50 4E

Column : Shodex IC SI-50 4E Eluent : 3.2mM Na2CO3 + 1.0mM NaHCO3 aq.Flow rate : 0.7mL/minDetector : Suppressed conductivityColumn temp.: 25˚C

NI-424 is a high-performance column whose number of theoretical plates is approximately twice as large as than that of I-524A.

<Features>(1) Enables the separation of H2PO4

- and F- ions which were difficult to separate with I-524A.

(2) The shape of each peak is sharper, and the separation balance is favorable. Improvement has been made especially in the separation of Cl- and NO2

- ions.

SI-50 4E is a high-performance version of SI-90 4E. Acetic acid, formic acid, and methacrylic acid are eluted more slowly than F- ion and faster than Cl- ion. The carbonate system peak appears between NO2

- and Br- peaks.

SI-52 4E is a high-resolution column exhibiting a number of theoretical plates of 14,000 or higher. It supports the simultaneous analysis of halogen oxides and inorganic anions as well as the high-sensitivity analysis of halogen oxides based on the suppressor method without using post-column detection. The recommended temperature for these analyses is 45˚C.

*CyDTA :

trans-1,2-Diaminocyclohexane-N,N,N',N'-tetra acetic acid

Sta

ndard

analysis

colu

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nio

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Anion analysis with suppressor method using VH-anion 4D

Courtesy of Mr. Heidger, Institut Heidger, Kesten, Germany

CH2OHCH2 CH2

CH2

OH

n=6,n=7,n=8,

α-CDβ-CDγ-CD

OH

O

On

OH OH

OH OH OHOH

OH

OH

OO

O

O

Structure of CD

22

Columns for Chiral Separation

Features

• α-, β- and γ-cyclodextrin derivatives bonded

• Separates chiral isomers based on conformity to cyclodextrin derivatives steric structure

• Silica-based and polymer-based packing materials

• General columns for chiral separation

• L-amino acid derivative bonded

• Separates chiral isomers by difference capacity of metal complex formation between sample and eluent

• For the separation of amino acids, hydroxylic acids, and derivatives thereof

• Bovine serum albumin (BSA) bonded

• Separates chiral isomers based on interactions between the isomers captured by the hydrophobic pockets of

BSA and surrounding amino acid residue

• For the separation of chiral isomers of amino acids and carboxylic acids

CD

CRX-853

ABA-894

Standard Columns

F7146000

F7146001

F7146002

F7146003

F6709250

F7140040

F6709300

F7118960

6

6

6

3

6

6

6

6

Column Size(mm)

I.D. x L

4.6 x 150

4.6 x 150

4.6 x 150

4.6 x 150

4.6 x 10

8.0 x 50

4.6 x 10

8.0 x 50

ORpak CDA-453 HQ

ORpak CDB-453 HQ

ORpak CDC-453 HQ

ORpak CDBS-453

ORpak CD-G

ORpak CRX-853

ORpak CRX-G

AFpak ABA-894


ParticleSize(μm)

Functional Group

α-Cyclodextrin derivative


γ-Cyclodextrin derivative


(guard column)

L-amino acid derivative

(guard column)

Bovine serum albumin

Base Material




Silica





Shipping Solvent

H2O

H2O

0.25mM CuSO4 aq.

0.25mM CuSO4 aq.


1.7% TEA-acetate buffer + 0.1M NaCl(pH4.0)/CH3CN=80/20

1.0% Acetic acid + 0.2M NaCl aq. /CH3CN=90/10

1.0% Acetic acid + 0.2M NaCl aq. /CH3CN=70/30

50mM Tris-HCl buffer +150mM NaCl+ 0.02% NaN3(pH7.4)

Colu

mns fo

r Chira

l Separa

tion

Sta

ndard

analysis

colu

mns

0 20 40 mim

0 20 40 mim

0 10 20 30 mim 0

1

2

3

10 20 30 mim

（Ⅰ）

（Ⅱ）

S-Warfarin（125μg）

4-(1-Oxo-2-cyclohexylmethyl)-benzoic acid

R-Warfarin（125μg）

Warfarin

OH

HO

HO

HO

OH

*

N

N

CH

6-Fluorotryptophan

Alanine-β-naphthylamide

MTH-Phenylalanine

DNS-Leucine

3.Didesmethyl-chlorpheniramine

1.Chlorpheniramine

2.Monodesmethyl-chlorpheniramine

CH3

CH3

CI

OH

CH3NH

O O

O O

OO

CHCH2COCH3*

CHCH2COCH3*

CHCH2COCH3*

6-hydroxy Warfarin（each 2.5μg）

6-hydroxy Warfarin7-hydroxy Warfarin（each 2.5μg）

7-hydroxy Warfarin

N CH

CH3

H

CI

N CH

H

H

CI

HN F

H2N CH CH2

H2N CH CONH

CH3

COOH

CH3

H3C

CH3

CH2

SO2NHCH COOH

CH

H3C

H3C

CH2O *

*

S

N

N

NH

*

*

NH2

Phenylalanine

DNS-Valine

Mandelic acid

CH2CH COOH

H3C CH3CH3

SO2NHCH COOH

CHH3C

*

N

1.98 1.33 AORpak

CDA-453 HQ

ORpakCDB-453 HQ

ORpakCDB-453 HQ

ORpakCDC-453 HQ

2.43 1.60 B

3.30 2.0 C

2.79 1.97 D

2.17 2.26 W

D

D

RS

L

L

ORpakCRX-853

AFpakABA-894

ORpakCRX-853

AFpakABA-894

1.58 1.43 X

2.30 1.44 Y

1.48 1.41 Z

OH

O

*

CH COOH

COOH

*

A : H2OB : [1.4% Acetic acid + 0.7% TEA + 0.1M NaCl(pH3.9)]/CH3CN=80/20C : (1.8% Acetic acid + 0.1M NaCl) aq./CH3CN=80/20D : (1.1% Acetic acid + 0.2M NaCl) aq./CH3CN=90/10

W : 0.5mM CuSO4 aq./CH3CN=60/40X : 25mM Phosphate buffer(pH7.0) + 0.5% Isopropanol Y : 0.25mM CuSO4 aq.Z : 50mM Sodium phosphate buffer(pH7.0)

N

N

Sample :

Sample : Epinephrine

Optical isomers Optical isomers

Am

ino

aci

ds

Am

ino

aci

ds

Hyd

roxy

aci

dsC

arbo

xylic

aci

ds

*

23

Medicinal chemicals Warfarin

Column :Shodex ORpak CDBS-453Eluent :(1% Acetic acid + 0.2M NaCl) aq./CH3CN=80/20Flow rate :0.4mL/minDetector :(Ⅰ) UV(310nm) (Ⅱ) Fruorescence(Ex 335nm, Em 415nm)Column temp. : 16.5˚C

Column :Shodex ORpak CDBS-453Eluent :(A) (1.1% Acetic acid+0.2M NaCl) aq. /CH3CN=90/10 (B) 2.2% Acetic acid+1.2% TEA + 0.2M NaCl aq. (C) (1.1% Acetic acid+0.2M NaCl) aq. /CH3CN=70/30Flow rate :(A, C) 0.5mL/min, (B) 1.0mL/minDetector :UV(254nm)Column temp.:20˚C

Chlorpheniramine and its methabolites

Amino acids, Hydroxy acids and Carboxylic acids

Rs ColumnEluentα

Rs eluentsample α

* Two asymmetric centers exist

Alprenolol

Brompheniramine

Caprofen

Chlorpheniramine

Chlorthalidone

Clidiniumbromide

Homatropine

Indapamide

Laudanosoline

Nefopam

Norlaudanosoline

Orphenadrine

Oxyphencyclimine

Procyclidine

Promethazine

Propranolol

Terbutaline

Tolperizone

Trihexyphenidyl

Valethamate*

　　　〃 *

Warfarin

Zopiclone

1.05

1.35

1.09

1.30

1.29

1.06

1.27

1.18

1.32

1.29

1.39

1.07

1.11

1.09

1.06

1.12

1.19

1.19

1.14

1.07

1.08

1.12

1.22

0.7

4.2

1.4

1.4

3.8

0.8

2.5

2.3

2.0

3.4

2.8

1.2

1.6

1.2

1.0

1.3

1.8

1.6

1.8

0.9

1.0

1.2

1.8

k,

3.3

4.4

4.5

0.6

6.8

9.8

4.3

10.6

7.6

2.1

7.6

16.0

17.4

5.8

15.5

18.6

1.5

21.3

6.1

3.2

3.9

3.1

1.8

A

A

C

C

A

A

B

A

B

A

B

A

C

C

A

B

A

B

C

C

C

C

A

Epinephrine

Column :Shodex ORpak CDBS-453Eluent : (0.05% Acetic acid + 0.2M NaCl) aq./CH3CN=99/1 Flow rate :0.5mL/minDetector :UV(254nm)Column temp.:10˚C

Rs ColumnEluentα

Column :Shodex ORpak CDBS-453Eluent : (1.0% Acetic acid + 0.2M NaCl) aq./CH3CN=90/10 Flow rate :0.5mL/minDetector :UV(254nm)Column temp.:30˚C

Amino acids

Courtesy of Dr.Sakurai,Tokyo University of Pharmacy and Life Science

Sta

ndard

analysis

colu

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tion

24

Columns for Saccharides analysis

Shodex analytical columns for saccharides analysis in ligand exchange, size exclusion, normal phase, and other chromatographic modes.* The list of saccharide elution volume for our columns is available.

Please refer to our website (http://www.shodex.com/) or technical notebook (No.2 and 3).

Features

• For simultaneous analysis of saccharides and organic acids• Separates saccharides in size exclusion mode, and organic acids in ion exclusion mode• Suitable for the analysis of uronic and aldonic acids

• Separates saccharides by combining ligand exchange and size exclusion modes• Three kinds of counter ions are available: Ca2+, Pb2+, and Na+ ions• Solvents other than water are unnecessary

• For the analysis of mannitol in conformity to USP (23rd ed.)

• Suitable for analyzing polysaccharides in size exclusion mode

• Separation is made by combining ligand exchange and normal phase modes• SC1211 can be used for the separation of sugar alcohols• SZ5532 is recommended for the separation of disaccharides and trisaccharides

• Polymer-based amino column is excellent in durability• The normal phase mode is ideal for the analysis of monosaccharides, oligosaccharides, and sugar alcohols• Suitable also for use with an evaporative light scattering detector and LC/MS

• Analyzes oligosaccharides in the size exclusion mode• For the analysis of small, water-soluble dietary fiber

SH1011SH1821

SC1011, 1821SP0810KS-801, 802

MN-431

KS-803~807

SC1211SZ5532

NH2P-50

GS-220 HQ


Notebook No.3

Notebook No.3

News No.6


News No.6, 14, 17, 32

Notebook No.2, 3

Notebook No.3

F6378100

F6378101

F6700080

SUGAR SH1011

SUGAR SH1821

SUGAR SH-G

6

6

10

H2O

H2O

H2O


8.0 x 300

8.0 x 300

6.0 x 50


ProductCode

Particle Size(μm)

Exclusion Limit(Pullulan)

≧ 17,000

≧ 17,000

(guard column)

1,000

10,000

—

Sulfo(H+)

Sulfo(H+)

Sulfo(H+)

CounterIon Shipping Solvent

Standard Columns

* Contact Shodex or our distributors near you for custom columns. Base Material : Styrene divinylbenzene copolymer

Size exclusion and Ion exclusion

F6378102

F6378103

F6700090

F6378105

F6700081

F6378010

F6378020

F6378025

F6378035

F6378050

F6378060

F6700020

F6378070

F6700021

F6379230

F6379300

SUGAR SC1011

SUGAR SC1821

SUGAR SC-LG

SUGAR SP0810

SUGAR SP-G

SUGAR KS-801

SUGAR KS-802

SUGAR KS-803

SUGAR KS-804

SUGAR KS-805

SUGAR KS-806

SUGAR KS-G

SUGAR KS-807

SUGAR KS-807G

USPpak MN-431

EP SC1011-7F

6

6

10

7

10

6

6

6

7

17

17

10

30

30

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O


8.0 x 300

8.0 x 300

6.0 x 50

8.0 x 300

6.0 x 50

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

6.0 x 50

8.0 x 300

8.0 x 50

4.0 x 250

7.5 x 300


ProductCode

Particle Size(μm)


≧ 13,000

≧ 13,000

(guard column)

≧ 11,000

(guard column)

≧ 17,000

≧ 17,000

≧ 17,000

≧ 17,000

≧ 9,000

≧ 9,000

(guard column)

≧ 4,000

(guard column)

1,000

10,000

—

1,000

—

1,000

10,000

50,000

400,000

5,000,000

(50,000,000)*

—

(200,000,000)*

—

Sulfo(Ca2+)

Sulfo(Ca2+)

Sulfo(Ca2+)

Sulfo(Pb2+)

Sulfo(Pb2+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)

Sulfo(Na+)


* See page 79 for the preparative columns for KS-800* Contact Shodex or our distributors near you for custom columns.

*( )Estimated valueBase Material : Styrene divinylbenzene copolymer

Ligand exchange and Size exclusion

Colu

mns fo

r Saccharid

es a

nalysis

Sta

ndard

analysis

colu

mns

See page 58 and 59 for details

See page 58 for details

25

Elution volume of various columns for saccharides analysis[Partial list only; refer to website for complete list]

Column : SUGAR SP0810, SC1011,KS-801Eluent : H2OFlow ｒate : 1.0mL/minDetector : RIColumn temp.: 80˚C

Column : SUGAR SZ5532Eluent : H2O/CH3CN=25/75Flow ｒate : 1.0mL/minDetector : RIColumn temp.: 60˚C

Column : SUGAR SC1211Eluent : H2O/CH3CN=65/35Flow ｒate : 1.0mL/minDetector : RIColumn temp.: 70˚C

Column : Asahipak NH2P-50 4EEluent : H2O/CH3CN=25/75Flow ｒate : 1.0mL/minDetector : RIColumn temp.: 30˚C

SP0810 SC1011 KS-801 SZ5532 SC1211 NH2P-504E

D(+)-Arabinose

D-Arabitol

2-Deoxy-D-glucose

Dulcitol

meso-Erythritol

D(—)-Fructose

D(+)-Fucose

D(+)-Galactose

Gentiobiose

Glucose

myo-Inositol

Isomaltose

Isomaltotriose

1-Kestose

Kojibiose

Lactitol

Lactose

Lactulose

Maltitol

Maltose

Maltotriose

8.86

10.42

15.86

8.83

20.18

12.70

11.05

10.48

9.74

7.22

8.63

12.77

7.68

7.09

6.79

7.56

13.27

8.05

9.13

12.23

7.85

7.48

7.75

8.91

11.33

7.58

12.76

10.09

8.85

8.84

7.98

6.08

7.30

8.86

6.26

5.75

5.75

6.21

8.09

6.51

6.99

8.26

6.34

5.89

6.68

8.21

7.63

7.15

7.40

7.86

7.71

8.09

7.58

5.75

7.17

7.99

5.95

5.34

5.26

5.88

6.13

5.99

6.19

6.03

5.94

5.38

—

5.11

7.27

4.34

9.46

5.73

5.37

4.50

6.46

10.50

5.87

12.63

10.57

21.17

13.09

9.65

16.35

10.12

9.16

13.04

8.67

13.79

4.10

5.56

8.16

4.02

11.28

6.27

5.90

4.96

4.98

*

4.76

7.87

*

*

*

*

6.67

4.07

4.65

6.77

*

*

6.66

6.18

6.29

6.02

7.45

5.43

6.75

5.43

8.10

16.36

8.61

9.96

15.18

27.55

20.11

14.82

11.82

13.27

10.72

11.82

14.24

24.96

N-Acetyl-α-D-glucosamine Mannitol

D-Mannose

Melibiose

Nystose

Palatinit

Palatinose

Panose

D(+)Raffinose

D(+)-Rhamnose

D(—)-Ribose

D(—)-Sorbitol

D(+)-Sorbose

Stachyose

Sucrose

α-D-Talose

Trehalose

Trehalulose

Xylitol

Xylobiose

D(+)-Xylose

D-Xylulose

15.80

10.72

8.16

11.13

6.38

2peaks

7.84

7.14

7.14

9.77

19.35

21.61

9.67

6.82

7.54

21.33

7.62

8.92

19.87

8.16

9.21

10.64

11.10

8.17

6.45

8.87

5.45

2peaks

6.45

5.78

5.78

8.23

13.66

13.31

8.03

5.57

6.29

12.59

6.27

6.95

13.14

6.68

7.09

9.02

7.23

7.64

5.98

7.78

4.93

5.90

5.89

5.32

5.29

7.37

9.04

7.42

7.38

4.97

5.87

8.76

5.78

6.10

7.94

6.40

7.71

8.04

8.75

5.83

11.69

3.99

20.05

2peaks

8.08

16.87

16.36

3.93

4.82

9.79

5.12

—

7.91

5.69

10.85

9.54

7.77

5.65

4.55

4.06

9.03

5.01

4.23

4.39

*

2peaks

3.99

*

*

4.43

8.64

11.88

4.92

*

*

8.51

*

4.78

10.16

*

4.48

5.07

7.39

7.84

14.70

4.71

31.90

12.73

12.12

25.60

20.25

5.52

5.45

7.09

7.35

36.22

11.87

6.47

13.25

11.68

6.10

9.05

6.58

5.41

Methyl-α-D-mannopyranoside

(—) Not detected (*) Overlap with solvent peak

SubstabcesElution Volume (mL)

SubstabcesElution Volume (mL)

F7001400

F6700120

F7001300

F6700110

SUGAR SC1211

SUGAR SC-G

SUGAR SZ5532

SUGAR SZ-G

6

10

6

6

H2O/CH3CN=75/25

H2O/CH3CN=75/25

H2O/CH3CN=30/70

H2O/CH3CN=30/70


6.0 x 250

4.6 x 10

6.0 x 150

4.6 x 10


ProductCode

Particle Size(μm)

≧ 5,500

(guard column)

≧ 5,500

(guard column)

Sulfo(Ca2+)

Sulfo(Ca2+)

Sulfo(Zn2+)

Sulfo(Zn2+)


* Contact our distributors near you for custom columns. Base Material : Styrene divinylbenzene copolymer

Ligand exchange and Normal phase

F7630002

F7630001

F6710016

F7630006

F6713000

F6710100

Asahipak NH2P-50 4D

Asahipak NH2P-50 4E


Asahipak NH2P-50 2D


Asahipak NH2P-LF

100

100

—

100

—

—

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75

H2O/CH3CN=25/75


4.6 x 150

4.6 x 250

4.6 x 10

2.0 x 150

2.0 x 10

8.0 x 75


ProductCode

Pore Size(Å)

5

5

5

5

5

—

Particle Size(μm)

≧ 5,000

≧ 7,500

(guard column)

≧ 3,500

(guard column)

(line filter)

Amino

Amino

Amino

Amino

Amino

Amino

FunctionalGroup Shipping Solvent

* See page 64 for the semi-micro columns and micro columns.* See page 77 for the preparative columns.* Contact our distributors near you for custom columns.


Normal phase

F7600005

F6710019

Asahipak GS-220 HQ

Asahipak GS-2G 7B

150

—

H2O/CH3OH=70/30

H2O/CH3OH=70/30


7.5 x 300

7.5 x 50


ProductCode

Pore Size(Å)

6

9

Particle Size(μm)

≧ 19,000

(guard column)

3,000

—

Exclution(Pullulan) Shipping Solvent

* See page 70 for the semi-micro columns and micro columns.* See page 80 for the preparative columns.* Contact our distributors near you for custom columns.


Size exclusion

SP0810 SC1011 KS-801 SZ5532 SC1211 NH2P-504E

(—) Not detected (*) Overlap with solvent peak

Sta

ndard

analysis

colu

mns

Colu

mns fo

r Saccharid

es a

nalysis

0

12

34

5

6・8

7

1

23

456

7

81

2 34

56

7

8

10 20 min 0 10 20 min

0 10 20 30 40 min

0 2

1

2

3

4

6 4 108 12 min

1

2

34

5

1

2

3

ClCH2OH

CH2Cl

CH2Cl

O

O

OOH

OH OH

OH

Sucralose

4

5

0

RI

UV（254nm）

10 15 min 5 10 20 min15

0

（A）70˚C

（B）25˚C

Glucose Galactose Mannose Xylose Arabinose

2 4 6min 0 2 4 6min 0 2 4 6min 0 2 4 6min 0 2 4 6 8min

Sample : 5μL1. Pullulan P10 0.5%2. Maltotriose 1.0%3. Maltose 1.0%4. Glucose 1.0%5. Sorbitol 1.0%6. Fructose 1.0%7. Glycerol 1.0%8. Ethanol 2.0%

Sample : 15μL1. Glucose 1.0% 2. Maltose 1.0% 3. Isomaltose 0.6% 4. Maltotriose 1.0%

β

β

β β

β

α

α

α

α

α

KS-801

SP0810

SC1011

12

3

4

0 5 10 15 20 min

26

Maltose and isomaltose Acesulfame K and saccharides Sucralose and saccharides

Column :Shodex SUGAR SZ5532 Eluent :H2O/CH3CN=25/75Flow rate :0.9mL/minDetector :RIColumn temp.:60˚C

Column :Shodex SUGAR SC1011Eluent :10mM CaSO4 aq.Flow rate :1.0mL/minDetector :RI, UV(254nm)Column temp.:80˚C

Column : Shodex SUGAR SP0810 Eluent : H2O Flow rate : 1.0mL/min Detector : RI Column temp.: 80˚C

Comparison of saccharides analysis based on different counter ions

Column :Shodex SUGAR SP0810 SC1011 KS-801Eluent :H2OFlow rate :0.6mL/minDetector :RIColumn temp.:80˚C

Effect of the separation of sugar anomers Pyridylaminated monosaccharides

Column :Shodex SUGAR SC1211 Eluent :H2O Flow rate :0.7mL/minDetector :RI Column temp.: (A)70˚C, (B)25˚C

Column :Shodex Asahipak NH2P-50 4E Eluent :H3PO4/H2O/CH3CN=1/14/85Flow rate :0.5mL/minDetector :Fluorescence (Ex : 310nm , Em : 380nm)Column temp.:40˚C

Saccharides may affect a chromatogram through anomer separation. When using a SUGAR column to analyze saccharides, performing the analysis at high temperatures would suppress the influence of anomer separation and result in a favorable chromatogram.

In the ligand exchange mode, the elution pattern depends on the kind of counter ion. Therefore, select the column applicable to the combination of saccharides to be analyzed.Refer list of "Elution volume of various columns for saccharides analysis" (p25)

Sample : 10μL1. Acesulfame K 0.005% 2. Sucrose 0.5% 3. Glucose 0.5% 4. Fructose 0.5%

Sample : 1% each, 10μL1. Sucrose2. Glucose3. Fructose4. meso-Erythritol5. Sucralose

Sample : 5μL 1. 2-Aminopyridine 0.1pmol/μL 2. PA-Rhamnose 1pmol/μL 3. PA-Fucose 1pmol/μL 4. PA-Ribose 1pmol/μL 5. PA-Xylose 1pmol/μL 6. PA-N-Acetylglucosamine 1pmol/μL 7. PA-N-Acetylgalactosamine 1pmol/μL 8. PA-Mannose 1pmol/μL 9. PA-Glucose 1pmol/μL10. PA-Galactose 1pmol/μL

0

1

2

3

4

56

7

9

8

10

20 40 60 min

Ketohexose

Column :Shodex SUGAR SP0810Eluent :H2OFlow rate :1.0mL/minDetector :RIColumn temp.:80˚C

Sample : 0.025% each, 10μL 1. Sorbose2. Fructose3. Tagatose4. Psicose

Sample : 1% each, 5μL

Colu

mns fo

r Saccharid

es a

nalysis

Sta

ndard

analysis

colu

mns

0 5

5

4

3

2

16

15 20 25 min10

10

0min

5 10 15 0

Dp=18

min5

H2O/CH3CN=50/50

(A) Isocratic elution (B) Gradient elution

H2O/CH3CN=50/50

H2O/CH3CN=43/57

10 15

15 20

10

Dp-10

Dp-5

Dp-1

15 20 min

25 30 35 40 50 min4

Mol

ecul

ar W

eigh

t (P

ullu

lan)

KS-801

KS-802

KS-805

KS-803

KS-804

KS-806

6 8

Elution volume (mL)

Volume（mL）

HA30 chromatogram by RI

MW data by MALS



HA12

127.0

—

116.5

90.9

Mw(*104)

recovery(%)

KS-807x2batch methodZimm plot

Mol

ar M

ass（

g/m

ol）

102

103

104

105

106

107

10 1245

16.0 20.0 24.012.01.0x103

1.0x104

1.0x105

1.0x106

1.0x107

1.0x108

1.0x109

HA20

208.5

—

191.5

89.1

Mw(*104)

recovery(%)


HA30

314.4

—

305.4

94.5

Mw(*104)

recovery(%)


27

Column :Shodex Asahipak NH2P-50 4EEluent :H2O/CH3CN=30/70Flow rate :1.0mL/minDetector :RIColumn temp.:25˚C

Fructooligosaccharide syrup Hydrolyzed dextran

Column : Shodex Asahipak NH2P-50 4EEluent : (A) H2O/CH3CN=50/50 (B) Linear gradient; H2O/CH3CN=43/57 to 50/50, 0 to 7min H2O/CH3CN=50/50, 7 to 16min Flow rate : 1.0mL/minDetector : ELS (Evaporative Light Scattering)Column temp.: 30˚C

Calibration curves for KS-800 using pullulan

Molecular weight distribution of Hyaluronic acids

Low molecular weight, water-soluble dietary fiber

Column :Shodex Asahipak GS-220 HQ x 2 Eluent :H2OFlow rate :0.5mL/min Detector :RIColumn temp.:60˚C

Column :Shodex SUGAR KS-802 x 2Eluent :H2OFlow rate :1.0mL/minDetector :RIColumn temp.:80˚C

Column :Shodex SUGAR KS-807 x 2Eluent :0.2M NaCl aq.Flow rate :0.5 mL/minDetector :RI, MALS (Multi Angle Laser Light Scattering) Column temp.:Room temp. (25˚C)

Sample : 100μLHyaluronic acid HA12 (Mw 1.2 x 106) 0.028% Hyaluronic acid HA20 (Mw 2.0 x 106) 0.026%Hyaluronic acid HA30 (Mw 3.0 x 106) 0.027%

Column :Shodex SUGAR KS-800 seriesEluent :H2ODetector :RIColumn temp.:80˚C

Hydrolyzed starch

NH2P-50 is excellent in durability and thus capable of carrying out gradient elution. The gradient elution enables rapid and clear separation of oligosaccharides.

Monosaccharides, disaccharides, and sugar alcohols are eluted with retention times larger than that indicated by the arrow. This facilitates the separation of those substances from indigestible fractions, making the determination of small, water-soluble dietary fiber more efficient.

KS-807 has an exclusion limit of 2·108, thereby enabling the determination of molecular weight distribution of hyaluronic acid that has a molecular weight of 1 million or larger. The collection rate is as good as approximately 90% or higher.

Sample : 20μLFructooligosaccharide syrup 2.5% 1. Fructose2. Glucose3. Sucrose 4. 1-Kestose5. Nystose6. 1-Fructofuranosyl-D-nystose

Sample : 20μLHydrolyzed starch 1%

Sample : Hydrolyzed dextran 0.5%, 20μL

Hyaluronic acid sample were offered by SHISEIDO

Sta

ndard

analysis

colu

mns

Colu

mns fo

r Saccharid

es a

nalysis

NEWNEW

28

Columns for Organic acids analysis

Features

• Ion exclusion mode (and reversed phase mode)• Separates a broad range of organic acids• Highly selective separation with post column method• KC-811 6E supports the analysis of cyanide ions and cyanogen chloride in accordance with the Japanese Water Supply Law

• For the analysis of highly hydrophobic organic acids

• Organic acids analysis in the reversed phase mode

KC-811

NN-814

DE-413

Standard Columns

F6378030

F6378033

F6700030

F6700010

F7008140

F6700510

F7001005

F6700150

RSpak KC-811

RSpak KC-811 6E

RSpak KC-G

RSpak KC-LG

RSpak NN-814

RSpak NN-G

RSpak DE-413

RSpak DE-G

≧ 17,000

≧ 13,000

(guard column)

(guard column)

≧ 9,000

(guard column)

≧ 11,000

(guard column)

6

6

10

12

10

10

4

10

Column Size(mm)

I.D. x L

8.0 x 300

6.0 x 250

6.0 x 50

8.0 x 50

8.0 x 250

6.0 x 50

4.6 x 150

4.6 x 10



ParticleSize(μm)

FunctionalGroup

Shipping Solvent

Sulfo

Sulfo

Sulfo

Sulfo

Sulfo

Sulfo

—

—

Base Material







Polymethacrylate

Polymethacrylate

0.1% H3PO4 aq.

0.1% H3PO4 aq.

0.1% H3PO4 aq.

0.1% H3PO4 aq.



H2O/CH3CN=50/50

H2O

* See page 64 for the semi-micro columns and micro columns for NN-814.* See page 79 for the preparative columns for KC-811.* Contact Shodex or our distributors near you for custom columns.

* When analyzing organic acids with KC-811, use KC-LG for samples with relatively low purity and KC-G for samples with relatively high purity as a guard column.

Notebook No.3 News No.6, 34

News No.7

News No.7

Columns for Amino acids analysis

Features

• Strong cation exchange mode• Supports the simultaneous analysis of different amino acids

• Multi mode (reversed phase and cation exchange) column that contains packing material with a trace amount of sulfo groups• Suitable for the analysis of selected amino acids• Amino acids are eluted in the order of acidity

P-421S

NN-814

Standard Columns

F6354211

F6700210

F7008140

F6700510

CXpak P-421S

CXpak P-G

RSpak NN-814

RSpak NN-G

≧ 3,500

(guard column)

≧ 9,000

(guard column)

6

6

10

10

Column Size(mm)

I.D. x L

4.6 x 150

4.6 x 10

8.0 x 250

6.0 x 50



ParticleSize(μm)

FunctionalGroup

Shipping Solvent

Sulfo(Na+)

Sulfo(Na+)

Sulfo

Sulfo

Base Material





H20

H20



* See page 64 for the semi-micro columns and micro columns for NN-814.* Contact Shodex or our distributors near you for custom columns.

News No.38

News No.7

Colu

mns fo

r Org

anic

acid

s analysis C

olu

mn

s for A

min

o a

cid

s an

alysis

Sta

ndard

analysis

colu

mns

10

10

9

8

7

6542

1

3

98765

42

1

3

11

12

13 14

20 30 min

10

987

6

5

4

2

1

3 987

5

6

4

21

3

810

7

6

542

3108

74 5

6

2

1

1

3

98

7

6

5

4

2

1

398

7

6

5

4

2

1

3

50 2015 25

30 min2520

30 35 40 min2520

98

7

6

5

4

21

3

0 1 2 3 4 5 6 7 8 min15

Eluent = 2mM HClO4 aq.

To shorten analysis time

Eluent = 2mM HClO4/CH3CN=90/10

Sample : 100μL1. Phosphoric acid etc.2. Citric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Lactic acid7. Fumaric acid8. Acetic acid9. Pyroglutamic acid

Sample : 2.5nmol each, 100μLSample : Soy-source (3 fold dilluted), 20μL 1. Phosphoric acid etc.2. Citric acid3. Pyruvic acid4. Malic acid5. Succinic acid

Sample : 1. Cl-,etc.2. Oxalic acid3. Maleic acid4. Citric acid5. Malic acid6. Vitamin C7. Succinic acid8. Formic acid9. Acetic acid

min1050 2015

min

100 20 30 40 50 min

Asp

Thr

Ser

Glu

Pro

Gly

Cys

Ala

Val

Met

IleLu

e Tyr

Phe

Lys

His

NH

3 Arg

1510 25 3020

25

min1510 25 3020

min

1050 2015 25min

105 20 min15

6. Lactic acid 7. Formic acid 8. Acetic acid 9. Levulinic acid10. Pyroglutamic acid

29

Organic acids in Japanese sake Organic acids and vitamin C in fruit juice

Column :Shodex RSpak KC-LG + KC-811 x 2Eluent :4.8mM HClO4 aq.Flow rate :1.0mL/minDetector :VIS(430nm) post column methodColumn temp.:63˚C

Column :Shodex RSpak KC-LG + KC-811 x 2Eluent :1mM HClO4 aq.Flow rate :1.0mL/minDetector :VIS(430nm) post column methodColumn temp.:40˚C

Standard organic acids

Fine rice wine

Highly hydrophobic organic acids Organic acids

Column :Shodex RSpak KC-811 x 2Eluent :6mM HClO4 aq.Flow rate :1.0mL/min Detector :VIS(430nm) post column methodColumn temp.:50˚C

Column :Shodex RSpak KC-LG + KC-811 x 2Flow rate :1.0mL/minDetector :VIS(430nm) post column methodColumn temp.:47˚C

Column : Shodex RSpak DE-413 Eluent : 10mM H3PO4 aq.Flow rate : 1.0mL/minDetector : RIColumn temp.: 50˚C

Sample : 5μL1. Glyoxylic acid 1.78mg/mL2. Tartaric acid 1.95mg/mL3. Malic acid 2.06mg/mL4. Lactic acid 2μL/mL5. Malonic acid 1.95mg/mL6. Acetic acid 2μL/mL7. Succinic acid 2.05mg/mL8. Levulinic acid 1.95mg/mL9. Propionic acid 2μL/mL

Organic acids in soy sauce Standard amino acids

Column :Shodex RSpak KC-LG + KC-811 x 2Eluent :4.8mM HClO4 aq.Flow rate :1.0mL/minDetector :VIS(430nm) post column methodColumn temp.:50˚C

Column :Shodex CXpak P-421S Eluent :No.1 0.12M Sodium citrate buffer(pH3.3) No.2 0.13M Sodium citrate buffer(pH3.2) No.3 0.11M Sodium citrate buffer(pH4.0) No.4 1.02M Sodium citrate buffer(pH4.9) No.5 0.2M NaOH aq./Ethanol=90/10 (Rinse solution) Low pressure gradient; No.1 (0min), No.2 (1.2min), No.3 (10.0min), No.4 (21.2min), No.5 (40min)Flow rate :0.5mL/minDetector :VIS(570nm) post column method (ninhydrin reaction 0.35mL/min, 120˚C)Column temp.:63˚C

Sample : 1. Citric acid 2. Tartaric acid 3. Pyruvic acid 4. Malic acid 5. Succinic acid 6. Glycolic acid 7. Lactic acid 8. Fumaric acid 9. Acetic acid10. Levulinic acid11. Pyroglutamic acid12. Propionic acid13. Isobutyric acid14. n-Butyric acid

Sample :1. Succinic 2. Lactic3. Formic4. Acetic5. Propionic6. Isobutyric7. n-Butyric8. Isovaleric9. n-Valeric

Pure rice wineFermented liquor

Light soy sauce Strong soy sauce

Sta

ndard

analysis

colu

mns

Colu

mns fo

r Org

anic

acid

s analysis C

olu

mn

s for A

min

o a

cid

s an

alysis

Polarity of Solvent

HighLow

Guideline of Availability

30

H2O Methanol DMSO DMF THF Chloroform TolueneAcetonitrile

KW-800 series, KW400 series

SB-800 HQ series, SB400 series

GS-HQ series

KD series

KF, K, LF series

GF-HQ series

44

50

44

38

38

48

48

48

48

48

46

46

44

42

Application Solvent PageColumn

Organic SEC(GPC)

Aqueous SEC(GFC)

Aqueous/Organic SEC

biological macromolecule (protein, peptide, nucleic acid etc.)

general synthetic polymer

polar polymer(polyimide, polyvinylpyrrolidone etc.)

analysis at high temperature (polyethylene, polypropylene etc.)

engineering resin analysis at room temperature[polyamide(nylon), polyethylene terephthalate (PET) etc.]

water-soluble polymer (polyacrylamide, polyethylenimine etc.), polysaccharide

biological macromolecule (high MW range)

oligosaccharide, polysaccharide

buffer etc. KW-800 series

KW400 series

SB-800 HQ series

SB400 series

SB-800 HQ series

KF-800 series

KF-600 series

KF-400HQ series

K-800 series

KD-800 series

SB-800 HQ series

SB400 series

HT-800 series

UT-800 series

AT-806MS

HFIP-800 series

HFIP-600 series

GF-HQ series

LF series

SB400 series

buffer etc.

buffer etc.

THF

THF

THF

THF

DMF

DMF

DMF

ODCB etc.

ODCB etc.

ODCB etc.

HFIP

HFIP

chloroform

H2O, bufferaqueous solution etc.

H2O, aqueous solution etc.

buffer etc.

36

36

38

38

38

38

28

40

Column selection for Size Exclusion Chromatography (SEC)

High performance (solvent-saving)



High linearity of calibration curve

Rapid analysis (solvent-saving)


Rapid analysis (solvent-saving)

KS-800 series

GS-HQ series

Colu

mn se

lectio

n fo

r Size

Exc

lusio

n C

hro

mato

gra

ph

y (SE

C)

Sta

ndard

analysis

colu

mns

31

Column Name

Shipping Solvent : THF

Shipping Solvent : Chloroform Shipping Solvent : THF

Shipping Solvent : DMF

THF

Chloroform

Carbon tetrachloride

Benzene

Toluene

p-Xylene

o-Dichlorobenzene(ODCB)

Trichlorobenzene(TCB)

Dioxane

Diethyl ether

Ethyl acetate

Acetone

Methyl ethyl ketone

Dimethylformamide(DMF)

Dimethylacetamide(DMAc)

Hexafluoroisopropanol(HFIP)

m-Cresol

o-Chlorophenol

Quinolin

N-Methylpyrrolidone(NMP)

Dimethylsulfoxide(DMSO)

30% m-Cresol/Chloroform

30% o-Chlorophenol/Chloroform

30% HFIP/Chloroform

Hexane

Acetonitrile

Methanol

Water

KF-804

KF-805

KF-806

KF-807

KF-806M

KF-805L

KF-806L

KF-807L

KF-601

KF-602

KF-602.5

KF-401HQ

KF-402HQ

KF-402.5HQ

KF-403HQ

KF-404HQ

KF-405LHQ

KF-406LHQ

KF-603

KF-604

KF-605

KF-606

KF-607

KF-606M

LF-804

LF-604

LF-404

KD-801

KD-802

KD-802.5

KD-804

KD-805

KD-806

KD-807

KD-806M

KD-803KF-801

K-801 K-803

KF-803KF-802

KF-802.5

KF-803L

KF-804L

K-802

K-802.5

K-803L

K-804L

K-804

K-805

K-806

K-807

K-806M

K-805L

K-806L

K-807L

*1

*1

*1

*1

*1

*1

*1

*1

*1

*1

*1

*1

*1

*1

Applicable to solvent replacementApplicable to solvent replacement but may cause the column performance to deteriorate.Use at 40˚C or higher.Inapplicable to solvent replacement

*1

Solvent

Applicability of SEC (GPC) columns to solvent replacement

Sta

ndard

analysis

colu

mns

Applic

ability o

f SE

C (G

PC

) colu

mns to

solve

nt re

pla

cem

en

t

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

32

400

1,000

1,500

—

150,000

700,000

(1,000,000)*

—

60,000

170,000

500,000

—

5

5

7

7

F6989000

F6989103

F6989104

F6700131

PROTEIN KW-802.5

PROTEIN KW-803

PROTEIN KW-804

PROTEIN KW-G

Aqueous SEC (GFC) columns : Silica-basedKW-800 are silica-based columns that have proven performance in the SEC analysis of proteins

KW400 columns are high-performance semi-micro columns with smaller particle size

Features

• Silica-based packing material for the aqueous SEC analysis.

• Suitable for the analysis of proteins and enzymes

• Reduced particle size has enhanced column performance.

• Three or four times higher sensitivity than KW-800 columns

• KW405-4F is applicable to samples with a molecular weight higher than 1 million.

KW-800

KW400

Standard Columns

Column Size(mm)

I.D. x LProduct Name

Exclusion Limit

(Pullulan) (Protein)ShippingSolvent

ProductCode

Particle Size(μm)

MaximumPore Size

(Å)

Particle Size(μm)

MaximumPore Size

(Å)


* See page 79 for the preparative columns.* Contact Shodex or our distributors near you for custom columns.

Base Material : SilicaUsable pH range : pH3.0~7.5The maximum usable concentration is 100% for both methanol and acetonitrile.


8.0 x 300

8.0 x 300

8.0 x 300

6.0 x 50

H2O

H2O

H2O

H2O

≧ 21,000

≧ 21,000

≧ 16,000

(guard column)

1,500(1,000,000)*50,000 7F6989106 PROTEIN KW-604S

For Column switching methods

Column Size(mm)


Exclusion Limit


ProductCode

Particle Size(μm)

MaximumPore Size

(Å)

* Contact Shodex or our distributors near you for custom columns. *( )Estimated value

6.0 x 50 H2O

400

800

1,500

2,000

—

150,000

600,000

(1,000,000)*

(20,000,000)*

—

60,000

150,000

500,000

1,300,000

—

3

3

5

5

5

F6989201

F6989202

F6989203

F6989204

F6700132

KW402.5-4F

KW403-4F

KW404-4F

KW405-4F

KW400G-4A

High performance semi-micro columns

Column Size(mm)


Exclusion Limit


ProductCode


* See page 67 for the semi-micro columns and micro columns.* Contact Shodex or our distributors near you for custom columns.

Base Material : SilicaUsable pH range : pH3.0~7.5The maximum usable concentration is 100% for both methanol and acetonitrile.*( )Estimated value

4.6 x 300

4.6 x 300

4.6 x 300

4.6 x 300

4.6 x 10

H2O

H2O

H2O

H2O

≧ 35,000

≧ 35,000

≧ 25,000

≧ 25,000

(guard column)

Aqueous S

EC

(GFC

) colu

mns : S

ilica-b

ase

dS

tandard

analysis

colu

mns

See page 82 forCalibration Standards

CalibrationStandards

(A) KW402.5-4F 12

3N=17,000

N=8,200

4

5

1 2 3 4

5

(B) KW-802.5

0

SB-803 HQ

GF-510 HQ

KW-803

0 5 10 15 20 25 3011 13 15 17 19 21 min

35

5 10 20 30 4015 min 50 min 0 5 10 min

1

23 4

5

102

1.5 2.52.0 3.0 4.03.5 4.5

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar W

eigh

t (P

rote

in)

5 7 9 116 8 10 12 13

Elution volume (mL)

Mol

ecul

ar W

eigh

t (P

rote

in)

KW404-4F

KW402.5-4F

KW403-4F

KW405-4F

KW-804

KW-802.5

KW-803

A

A

A

B

B

B

C

C

C

D

3

1

2

3

4

5

6

7

8

6

D

D

E

MW

104

105

106

E

E

min

SB-803 HQGF-510 HQ

KW-803

10 15 10 15 2020 min 30 min

102

103

104

105

106

107

33

Lectins

Recovery rate of proteins

Column :Shodex KW402.5-4FEluent :50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)Flow rate :0.33mL/minDetector :UV(220nm) (small cell volume)Column temp.:30˚C

Sample : 10μL1. Blue dextran 2000 0.2mg/mL2. γ-Globulin 0.8mg/mL3. Ovalbumin 0.8mg/mL4. Myogrobin 0.56mg/mL5. Uridine 0.04mg/mL

Sample : A : Thyroglobulin (bovine)B : γ-Globulin (bovine)C : Ovalbumin (chicken)D : Myoglobin (horse)E : Cyanocobalamin

Sample : Whey, 5μL Sample : 5μL1. Lectin from Soybean 0.6mg/mL2. Lectin from Arachis hypogaea 1.1mg/mL3. Lectin from Canavalia ensiformis (Con A) 0.9mg/mL4. Lectin from Lens culinaris (LCA) 0.7mg/mL5. Lectin from Triticum vulgaris (WGA) 0.8mg/mL

Sample : Human serum 0.1% each1. Fibrinogen 50μL2. α2 -Macroglobulin 50μL3. IgG 50μL4. Transferrin 50μL5. Plasminogen 50μL6. Albumin 100μL7. Antitrypsin 100μL8. Hemoglobin 100μL

Comparison of KW402 and KW-802.5 Milk whey in yogurt

Column : (A) Shodex KW402.5-4F (B) Shodex PROTEIN KW-802.5Eluent :50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)Flow rate : (A) 0.33mL/min, (B) 1.0mL/min Detector :UV(280nm) (small cell volume)Column temp.:25˚C

Column :Shodex OHpak SB-803 HQ, Asahipak GF-510 HQ, PROTEIN KW-803 Eluent :0.2M Phosphate buffer(pH6.9)Flow rate :0.5mL/minDetector :UV(280nm)Column temp.:30˚C

Column :Shodex PROTEIN KW-803 x 2Eluent :50mM Phosphate buffer+ 0.3M NaCl(pH7.0) Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Column :Shodex KW402.5-4F + KW403-4F Eluent :50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)Flow rate :0.20mL/minDetector :UV(280nm) (small cell volume)Column temp.:30˚C

Column :Shodex KW402.5-4F, KW403-4F Eluent :50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)Flow rate :0.33mL/min Detector :UV(280nm) (small cell volume)Column temp.:25˚C

Column :Shodex KW400-4F series, Shodex PROTEIN KW-800 seriesEluent :50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0) Flow rate : (KW400) 0.33mL/min (KW-800) 1.0mL/minDetector : (KW400) UV(280nm) (small cell volume) (KW-800) UV(280nm) (conventional type)Column temp.:25˚C

Calibration curves for KW400 series and KW-800 series

Proteins in human blood serumComparison of standard protein separation using various GFC columns

ProteinRecovery (%)

γ- Globulin


Ovalbumin

Myoglobin

Cytochrome c

Lysozyme

α-Chymotrypsinogen A

98

89

89

90

92

87

95

96

96

97

89

92

98

94

KW402.5-4F KW403-4F

SEC columns for an aqueous system, i.e., SB-803 HQ, GF-510 HQ and KW-803, were compared with each other in terms of the separation of standard proteins. The results demonstrate that KW-803, a silica-based column, is the most suitable for such an application.

KW400 series is high-performance semi-micro columns that have a number of theoretical plates approximately 1.5 times larger and a detection sensitivity (peak height) that is 3 to 4 times higher than what KW-800 columns have.

Sta

ndard

analysis

colu

mns

Aqueous S

EC

(GFC

) colu

mns : S

ilica-b

ase

d

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

NEWNEW

34

Aqueous SEC (GFC) columns : Polymer-basedSB-800 HQ are polyhydroxymethacrylate-based SEC columns for the molecular weight distribution analysis of water-soluble polymers.The solvent can be substituted with DMF (in columns other than SB-802 HQ and SB-807 HQ), enabling the analysis of polar polymers.

Features

• Polymer-based SEC (GFC) column for an aqueous system• Supports a wide range of molecular weight• SB-807 HQ with an exclusion limit of 500,000,000 is available for the analysis of macromolecules.• The solvent can be substituted with DMF (in columns other than SB-802 HQ and SB-807 HQ), enabling the analysis of polar polymers

• Three or four times higher sensitivity than the SB-800 HQ columns• SB401-4E can analyze small molecules with MW of under 1,000

SB-800 HQ

SB400

F6429100

F6429101

F6429102

F6429103

F6429104

F6429105

F6429106

F6709430

F6429108

F6709431

OHpak SB-802 HQ

OHpak SB-802.5 HQ

OHpak SB-803 HQ

OHpak SB-804 HQ

OHpak SB-805 HQ

OHpak SB-806 HQ

OHpak SB-806M HQ

OHpak SB-G

OHpak SB-807 HQ

OHpak SB-807G

Standard Columns

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

6.0 x 50

8.0 x 300

8.0 x 50

Product NameProduct

Code Shipping Solvent

0.02% NaN3 aq.

0.02% NaN3 aq.

0.02% NaN3 aq.

0.02% NaN3 aq.

0.02% NaN3 aq.

0.02% NaN3 aq.

0.02% NaN3 aq.

0.02% NaN3 aq.

H2O

H2O

* See page 68, 69 for the semi-micro columns and micro columns.* See page 79 for the preparative columns.* Contact Shodex or our distributors near you for custom columns.

Base Material : PolyhydroxymethacrylateUsable pH range : pH3~10*( )Estimated value

News No.39



≧ 12,000

≧ 16,000

≧ 16,000

≧ 16,000

≧ 12,000

≧ 12,000

≧ 12,000

(guard column)

≧ 1,500

(guard column)

4,000

10,000

100,000

1,000,000

(4,000,000)*

(20,000,000)*

(20,000,000)*

—

(500,000,000)*

—

8

6

6

10

13

13

13

10

35

35

100

200

800

2,000

7,000

15,000

15,000

—

30,000

—


Particle Size(μm)

MaximumPore Size

(Å)

F6429111

F6429112

F6429113

F6429114

F6709432

SB401-4E

SB402.5-4E

SB403-4E

SB404-4E

SB400G-4A

SB401-4E

SB402.5-4E

SB403-4E

SB404-4E

SB400G-4A

SB-802 HQ

SB-802.5 HQ

SB-803 HQ

SB-804 HQ

SB-805 HQ

SB-806 HQ

SB-806M HQ

SB-G

SB-807 HQ

SB-807 G

High performance semi-micro columns

Usable concentration of organic solvents

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 10

Product Name

Product NameMethanol Acetonitrile DMF

The maximum usable concentration (%)

ProductCode

Shipping Solvent

H2O

H2O

H2O

H2O

H2O

* Contact Shodex or our distributors near you for custom columns. Base Material : PolyhydroxymethacrylateUsable pH range : pH3~10



≧ 5,000

≧ 20,000

≧ 20,000

≧ 20,000

(guard column)

1,000

10,000

100,000

1,000,000

—

10

6

6

7

7

40

200

800

2,000

—


Particle Size(μm)

MaximumPore Size

(Å)

Standard columns High performance semi-micro columns

0

100

100

75

75

75

75

75

30

30

0

75

75

75

75

75

75

75

30

30

0

100

100

100

100

100

100

100

0

0

Product NameMethanol Acetonitrile DMF

The maximum usable concentration (%)

0

100

100

75

75

10

75

75

75

75

0

100

100

100

100

Aqueous S

EC

(GFC

) colu

mns : P

olym

er-b

ase

dS

tandard

analysis

colu

mns



SB-806 HQ

Mw: 5,500,000

4.0

1.0x105

1.0x106

6.0 8.0 10.0 12.0 14.0

Volume(mL)

1.0x107

1.0x108

Mol

ar M

ass(

g/m

ol)

SB-807 HQ

8.0

0.2 M

0.1 M 0.02 M

0.05 M

12.0 16.0 20.0 24.0

0

min

CH3

CH3

Volume (mL)

min

5

8 12 16 20 min

0 2 4 6 810

3

21

10 12 14 16 18 20 22 24 26 28 30

O O

On

CC

4 5 6 7 8 9 10 11 12

103

102

104

105

106

107

Elution volume (mL)

SB-806 HQSB-805 HQSB-804 HQ

SB

-806M H

Q

SB-803 HQ

SB-802.5 HQSB-802 HQSB401-4E

SB402.5-4E

SB403-4E SB

404-4E

Mol

ecul

ar w

eigh

t (P

ullu

lan)

102

4 5 6 7 8 9 10 11 12

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

EG

/PE

O) SB

-806 HQ

SB-805 HQSB-804 HQSB

-806M H

Q

SB-803 HQ

SB-802.5 HQ

102

0.5 1.5 2.51.0 2.0 3.0 3.5

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

ullu

lan)

Mw=101 x 104

Mn=10.5 x 104

Mw/Mn=9.6

min

0 10 20 30 40 50

3

4521

35

Sample : 10μL1. Gly-Gly-Gly2. Gly-Gly3. Glycine

Sample : Polycarbonate

Sample : Polyarylamine 0.2%, 100μL

Sample : Carboxymethylcellulose　　　　 (Medium viscosity) 0.1%, 200μL

Polycarbonate Polyacrylamide

Column :Shodex SB400 series, Shodex OHpak SB-800 HQ seriesEluent :H2O Flow rate :0.3mL/min, 1.0mL/minDetector : (SB400) RI(small cell volume) (SB-800 HQ) RI(conventional type)Column temp.:Room temp.

Column :Shodex OHpak SB-800 HQ series Eluent :20mM LiBr in DMFFlow rate :0.8mL/minDetector :RIColumn temp.:40˚C

Column :Shodex SB401-4EEluent :50mM CH3COONH4 aq. /CH3CN=90/10Flow rate :0.2mL/minDetector :UV(210nm) (small cell volume) Column temp.:25˚C

Column :Shodex OHpak SB-806M HQ x 2 Eluent :0.5M Acetic acid + NaNO3 aq.Flow rate :1.0mL/min Detector :RI Column temp. : 40˚C

Column :Shodex OHpak SB-805 HQ + SB-802.5 HQ Eluent :5mM LiBr in DMFFlow rate :1.0mL/minDetector :RIColumn temp.:40˚C

Column :Shodex OHpak SB-807 HQ, SB-806 HQEluent :0.2M NaCl aq.Flow rate :0.5mL/minDetector :RI MALS (Multi angle laser light scattering) Column temp. : 30˚C

Column :Shodex OHpak SB-806M HQ x 2Eluent :0.1M NaCl aq.Flow rate :1.0mL/minDetector :RIColumn temp.:40˚C

In the analysis of cationic polymers such as polyarylamine, the concentration of sodium nitrate in the eluent being 0.02 M would allow the column to adsorb the main chain of polyarylamine, resulting in the complete prevention of the elution of polyarylamine. However, if the concentration is 0.1 M or higher, adsorption of the sample is suppressed and a favorable chromatogram can be obtained.

Oligopeptides Carboxymethylcellulose

Calibration curves for SB400 series and SB-800 HQ series using pullulan (H2O)

Sample : 5μL1. Maltopentaose2. Maltotetraose3. Maltotriose4. Maltose5. Glucose

Column :Shodex SB401-4E x 2Eluent :H2OFlow rate :0.1mL/minDetector :RI(small cell volume) Column temp.:70˚C

Maltooligosaccharide

Sta

ndard

analysis

colu

mns

Aqueous S

EC

(GFC

) colu

mns : P

olym

er-b

ase

d

Effect of sodium nitrate concentration in eluent on the analysis of polyallylamine

Calibration curves for SB-800 HQ series using PEG and PEO (DMF)

36

Asahipak GS-220 HQ

Asahipak GS-320 HQ

Asahipak GS-520 HQ

Asahipak GS-620 HQ

Asahipak GS-2G 7B

Multi mode columns

Multi mode GS columns separates by SEC, distribution/adsorption and ion exchange modes, depending on

the eluent conditions.

Features

• Polyvinylalcohol-based SEC column for aqueous system

• For the separation of peptides or nucleic acids that have similar molecular weights

• For desalting a sample or substituting buffer in protein analysis.

GS-HQ

Standard Columns

Product Name

F7600005

F7600006

F7600007

F7600008

F6710019

ProductCode

News No.19, 20


≧ 19,000

≧ 19,000

≧ 18,000

≧ 18,000

(guard column)


Base Material : Polyvinyl alcoholUsable pH range : pH2~12(GS-120 HQ, GS-220 HQ : pH 2~9)Usable concentration of methanol is up to 100% (GS-220 HQ up to 30%)Usable concentration of acetonitrile is up to 50%* PEG equivalent**( )Estimated value

7.5 x 300

7.5 x 300

7.5 x 300

7.5 x 300

7.5 x 50

Shipping Solvent

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30


3,000*

40,000

300,000

(2,000,000)**

—

6

6

7

7

9

150

400

2,000

7,000

—


Particle Size(μm)

MaximumPore Size

(Å)

Multi m

ode c

olu

mns

Sta

ndard

analysis

colu

mns



GFC

Mol

ecul

ar w

eigh

t

3

2

1

0 10 20 30 min

0

1

2 3

4

5

7

6

8

9

10

10 20 30 min 10

1

2 3

15 20 25 min 10

1

2

34 5

6

20 30

0.03mV

0.02mV

0.1mV

min

min

0 5 10 15 20 25

15 20 25

1

1

103

102

23

4

5

6

2

3

4

5

6

10 15 20 25 min

GS320A-1E

GS320A-2E

GS-320 HQ

min

102

4 5 6 7 8 9 10 11 12

103

104

105

106

Elution volume (mL)

Mol

ecul

ar w

eigh

t

GS-220 HQ

GS-320 HQ

GS-520 HQ

GS-620 HQ

PullulanPEG

37

Sample : 1. ATP 2. ADP 3. AMP 4. IMP 5. GMP 6. Adenine 7. Adenosine 8. Inosine 9. Guanine10. Hypoxanthine

Sample : 100mg/L each 1. 2'AMP2. 3'AMP3. cAMP

Sample : Polyphosphates1. Sodium methaphosphate, (NaPO3)n2. Sodium tetrapolyphosphate hexabasic, Na6P4O133. Sodium triphosphate pentabasic, Na5P3O104. Sodium pyrophosphate tetrabasic, Na4P2O75. Sodium phosphate tribasic, Na3PO46. NaCl

Nucleobases, nucleosides and nucleotides cyclic AMP and AMPs Polyphosphates

Column :Shodex Asahipak GS-320 HQEluent :200mM Sodium phosphate buffer (pH3.0)Flow rate :0.6mL/minDetector :UV(260nm)Column temp.:30˚C

Column :Shodex Asahipak GS-320 HQ Eluent :200mM Sodium phosphate buffer (pH5.0)Flow rate :1.0mL/minDetector :UV(260nm)Column temp.:30˚C

Column :Shodex Asahipak GS-220 HQ x 2Eluent :50mM NaCl aq.Flow rate :0.6mL/minDetector :RIColumn temp.:60˚C

Sample : 0.5% each, 20μL1. β-Endorphin2. α-Endorphin3. γ-Endorphin

Sample : Uracil 10mg/L, 1μL

peak heightHQ : 2E : 1E = 1 : 12 : 36

Sample : 100nLData was provided by Dr.Ogra, Chiba University.

Σf : Hydrophobic parameterpl : Isoelectric point

Endorphin

Column :Shodex Asahipak GS-320 HQEluent :0.1% TFA aq./CH3CN=95/5Flow rate :0.8mL/minDetector :UV(210nm)Column temp.:30˚C

Column :Shodex Asahipak GS-HQ seriesEluent : H2OFlow rate :0.6mL/minDetector :RIColumn temp.:30˚C

Peptides

Column : Shodex Asahipak GS-320 HQEluent : 50mM Ammonium acetate buffer(pH7.0)Flow rate : 0.5mL/minDetector : UV(220nm)Column temp.: 30˚C

Eluent : 10mM Ammonium formate buffer (pH4.0)Detector : UV(260nm)

Column : Shodex GS320A-M8E Eluent : 50mM Ammonium acetate buffer (pH6.5) Flow rate: 5.5μL/minDetector : ICP-MS(Se at m/z=82)

GS-HQ columns can be used not only for SEC (GFC) in an aqueous system, but also for multimodal analysis where hydrophobic interaction and ionic interaction are used together as separation criteria, under certain conditions of the eluent. This realizes unprecedented separation analysis.GS-HQ columns are excellent in the performance of separating hydrophilic peptides, in particular, acidic or basic peptides, from each other.

plΣf

Val-Glu-Glu-Ala-Glu

Glu-Ala-Glu

Val-Glu-Ser-Glu

Arg-Asp

Gly-His-Lys

Arg-Pro-Lys-Pro

1.78

0.39

0.83

0.68

0.29

3.24

3.02

3.12

3.12

6.75

9.95

11.44

MW

576

347

390

289

340

497

1

2

3

4

5

6

No.

Sample :

Comparison for baseline

GS320A-1E(1.0mmIDx250mm)Flow rate:10μL/min

GS320A-2E(2.0mmIDx250mm)Flow rate:40μL/min

GS-320 HQ(7.5mmIDx300mm)Flow rate:600μL/min

NH2

NH2

HOOC

HOOC

MeSeCys

SeMet

Methylselenocysteine (MeSecys)

Selenomethionine (SeMet)

Se

Se

Sta

ndard

analysis

colu

mns

Multi m

ode c

olu

mns

Calibration curves for GS-HQ series using pullulan and PEG

Comparison of semi-micro columns for sensitivity

High sensitive analysis of metal-biding amino acids

38

0~0.5M

0~100%

0~100%

0~100%

0~100%

0~100%

0~100%

0~100%

0~100%

0~50%

51~100%

Asahipak GF-310 HQ

Asahipak GF-510 HQ

Asahipak GF-710 HQ

Asahipak GF-7M HQ

Asahipak GF-310 HQ 8D

Asahipak GF-1G 7B

Water (sodium concentration)

Methanol

Ethanol

Acetonitrile

THF

DMF

Acetone

Chloroform

Ethyl acetate

DMSO

DMSO

Aqueous/Organic SEC columns

The GF series are SEC columns with excellent solvent resistance.Both water and organic solvents can be used. High performance analysis of samples containing hydrophilic and hydrophobic parts, such as surfactants.

Features

• Polymer-based SEC column with excellent solvent resistance

• Supports both aqueous and organic solvent systems

GF-HQ

Standard Columns

Usable solvents

Product Name

F7600001

F7600002

F7600003

F7600004

F7600090

F6710018

ProductCode

News No.40


≧ 19,000

≧ 19,000

≧ 11,000

≧ 13,000

≧ 10,000

(guard column)


Base Material : Polyvinyl alcoholUsable pH range : pH2~9*( )Estimated value

7.5 x 300

7.5 x 300

7.5 x 300

7.5 x 300

8.0 x 150

7.5 x 50

Shipping Solvent

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30

H2O/CH3OH=70/30


40,000

300,000

(10,000,000)*

(10,000,000)*

40,000

—

5

5

9

9

5

9

400

2,000

10,000

10,000

400

—


Particle Size(μm)

MaximumPore Size

(Å)

Aqueous/O

rganic

SE

C c

olu

mns

Sta

ndard

analysis

colu

mns



0 10 20 30 40 min

0 10 20 30 40 min

0 10 20 30 40 min

1

2

3

4

5 6

1

2

3

4

56

1

23

4

56

0 20 min 0 20 min 0 20 min

0 10 20 30 min

0 10 20

0

5 10 0 5 10 15 20 25 30 35 40 min15 min

10 20 30 min

0

1

1

2

2

3

3

4

5 10 15 min

min

102

4 5 6 7 8 9 10 11

103

104

105

106

Elution volume (mL)

Mol

ecul

ar w

eigh

t PullulanPEG

GF-310 HQ

GF-510 HQ

GF-7M

HQ

GF-710 HQ

102

4 5 6 7 8 9 10 11

103

104

105

106

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

olys

tyre

ne)

GF-710 HQ

GF-310 HQ

GF-510 HQ

GF-7M

HQ

O

O OCHC

HO CH2OH

O

OCHO

CH2OH

O

OC

CH2OH

39

Sample : 0.25% each, 100μL 1. Tristearin2. Trilaurin3. Tributylin4. Triacetin

Sample : 1mg/mL each, 50μLPolystyrene1. MW :1,090,0002. MW : 190,0003. MW : 37,9004. MW : 9,1005. MW : 2,9806. MW : 500

Sample : 1mg/mL each, 50μLPoly(sodium styrenesulfonate)

Sample : 50μL1. Aldosterone 0.125mg/mL2. Corticosterone 0.25mg/mL3. Deoxycorticosterone 0.25mg/mL

Sample : Polyacrylonitrile 0.1%(w/v), 100μL

Adrenal cortical hormone Triglyceride Polyacrylonitrile

Column :Shodex Asahipak GF-510 HQ Eluent :H2O/CH3CN=55/45Flow rate :0.5mL/minDetector :UV(240nm)Column temp.:30˚C

Column :Shodex Asahipak GF-310 HQEluent :THFFlow rate :0.6mL/minDetector :RIColumn temp.:30˚C

Column :Shodex Asahipak GF-710 HQ x 2Eluent :20mM LiBr in DMFFlow rate :0.6mL/minDetector :RIColumn temp.:40˚C

Comparison of standard polystyrene separation using various solvent Poly (sodium styrenesulfonate)

Column :Shodex Asahipak GF-310 HQ + GF-510 HQ Eluent : (A): THF, (B): Chloroform, (C): DMFFlow rate :0.5mL/minDetector : (A),(B) UV(254nm), (C) UV(270nm)Column temp.:30˚C

Column :Shodex Asahipak GF-510 HQ Eluent :50mM LiCl aq./CH3CN =60/40Flow rate :0.6mL/minDetector :UV(254nm)Column temp.:30˚C

Polymers having both hydrophobic and hydrophilic moieties may exhibit hydrophobic interaction with packing materials. When using such polymers, SEC analysis can be performed by eliminating the hydrophobic interaction through the addition of organic solvent to the eluent.

Although GF-HQ columns operate mainly in the SEC mode, they may also be used for the separation of substances that have similar molecular weights based on the strength of hydrophobic interaction under

certain conditions of the eluent.

N-methoxymethyl Nylon

Column :Shodex Asahipak GF-HQ seriesEluent :H2OFlow rate :0.6mL/minDetector :RIColumn temp.:30˚C

Column :Shodex Asahipak GF-HQ seriesEluent :ChloroformFlow rate :0.6mL/minDetector :UV(254nm)Column temp.:30˚C

Column :Shodex Asahipak GF-710 HQ x 2Eluent :50mM LiCl in CH3OHFlow rate :0.5mL/minDetector :RIColumn temp.:30˚C

(A) THF (B) Chloroform (C) DMF

Mw=63,000Mn=18,000Mw/Mn=3.4

Mw=52,000Mn=17,000Mw/Mn=3.1

Mw=29,000Mn=11,000Mw/Mn=2.7

Sample : 10mg/mL each, 60μLN-Methoxymethyl Nylon®

~CH2 CH2~

CH2OCH3

CH2 CH

n SO3Na

N C

O

MW:104,000

MW:38,000

MW:8,000

1. Aldosterone

2. Corticosterone

3. Deoxycorticosterone

Sta

ndard

analysis

colu

mns

Aqueous/O

rganic

SE

C c

olu

mns

Calibration curves for GF-HQ series using pullulan and PEG

Calibration curves for GF-HQ series using polystyrene

40

Organic SEC (GPC) columns : General AnalysisThis series includes standard SEC columns with proven performance. Columns are shipped in tetrahydrofuran (THF), chloroform, or dimethylformamide (DMF).

Standard Columns (8.0 mm I.D. x 300 mm)

GPC KF-801

GPC KF-802

GPC KF-802.5

GPC KF-803

GPC KF-803L

GPC KF-804

GPC KF-804L

GPC KF-805

GPC KF-805L

GPC KF-806

GPC KF-806M

GPC KF-806L

GPC KF-807

GPC KF-807L

GPC KF-G

GPC KF-800D

Product Name

F6028010

F6028020

F6028025

F6028030

F6027030

F6028040

F6027040

F6028050

F6027050

F6028060

F6028090

F6027060

F6028070

F6027070

F6700300

F6709350

Product CodePlate Number(TP/column)

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 11,000

≧ 11,000

≧ 11,000

≧ 13,000

≧ 11,000

≧ 6,000

≧ 6,000

(guard column)

(solvent-peak separation column)


1,500

5,000

20,000

70,000

70,000

400,000

400,000

4,000,000

4,000,000

(20,000,000)*

(20,000,000)*

(20,000,000)*

(200,000,000)*

(200,000,000)*

—

—

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

4.6 x 10

8.0 x 100


6

6

6

6

6

7

7

10

10

10

10

10

18

18

8

10

Particle Size(μm)

50

150

300

500

500

1,500

1,500

5,000

5,000

10,000

10,000

10,000

20,000

20,000

—

—

MaximumPore Size

(Å)

MaximumPore Size

(Å)

MaximumPore Size

(Å)

KF-800 series : Tetrahydrofuran(THF)

GPC K-801

GPC K-802

GPC K-802.5

GPC K-803

GPC K-803L

GPC K-804

GPC K-804L

GPC K-805

GPC K-805L

GPC K-806

GPC K-806M

GPC K-806L

GPC K-807

GPC K-807L

GPC K-G

GPC K-800D

Product Name

F6028110

F6028120

F6028125

F6028130

F6028194

F6028140

F6028195

F6028150

F6028196

F6028160

F6028190

F6028197

F6028170

F6028198

F6700401

F6709450


≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 11,000

≧ 11,000

≧ 11,000

≧ 13,000

≧ 11,000

≧ 6,000

≧ 6,000

(guard column)



1,500

5,000

20,000

70,000

70,000

400,000

400,000

4,000,000

4,000,000

(20,000,000)*

(20,000,000)*

(20,000,000)*

(200,000,000)*

(200,000,000)*

—

—

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

4.6 x 10

8.0 x 100


6

6

6

6

6

7

7

10

10

10

10

10

18

18

8

10

Particle Size(μm)

50

150

300

500

500

1,500

1,500

5,000

5,000

10,000

10,000

10,000

20,000

20,000

—

—

K-800 series : Chloroform*( )Estimated value

*( )Estimated value

GPC KD-801

GPC KD-802

GPC KD-802.5

GPC KD-803

GPC KD-804

GPC KD-805

GPC KD-806

GPC KD-806M

GPC KD-807

GPC KD-G

Product Name

F6028210

F6028220

F6028225

F6028230

F6028240

F6028250

F6028260

F6028290

F6028270

F6700411


≧ 17,000

≧ 17,000

≧ 17,000

≧ 17,000

≧ 17,000

≧ 11,000

≧ 11,000

≧ 13,000

≧ 6,000

(guard column)


2,500

5,000

20,000

70,000

400,000

4,000,000

(20,000,000)*

(20,000,000)*

(200,000,000)*

—

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

4.6 x 10


6

6

6

6

7

10

10

10

18

8

Particle Size(μm)

50

150

300

500

1,500

5,000

10,000

10,000

20,000

—

KD-800 series : Dimethylformamide(DMF)

*( )Estimated value* See page 50 for the solvent-peak separation columns.* See page 80, 81 for the preparative columns.* Contact Shodex or our distributors near you for custom columns.

Product names with "L" represent "linear columns," which exhibit linearity in the calibration curve over a wide range of molecular weights and thus are easy-to-use SEC columns. The use of "linear columns" is recommended when compared to other columns that have the same exclusion limit.

Org

anic

SE

C (G

PC

) colu

mns : G

enera

l Analysis

Sta

ndard

analysis

colu

mns

102

105 6 7 8 9 10 11 12 13

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

olys

tyre

ne)

KF-801

KF-802

KF-802.5

KF-803

KF-803L

KF-804L

KF-807L

KF-806L

KF-805L

KF-804

KF-805

KF-806

KF-807

KF-806M

102

1065 7 8 9 10 11 12

103

104

105

106

107

Elution volume (mL)M

olec

ular

wei

ght

(Pol

ysty

rene

)

13

102

106 7 8 9 10 11 12

103

104

105

106

107

108108108

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

EG

/PE

O)

KD-801

KD-802

KD-802.5

KD-803

KD-804

KD-805

KD-807KD-806KD-806M

25 min 15 20 300 10 20 30minmin

201510

(A)

(B)

(C)

25 min201510

20

min min

251515 20 25 30

41

Column :Shodex GPC KF-800 seriesEluent :THFFlow rate :1.0mL/minDetector :RIColumn temp.:Room temp.

Column :Shodex GPC KF-800L series Eluent :THFFlow rate :1.0mL/minDetector :RIColumn temp.:Room temp.

Column :Shodex GPC KD-800 seriesEluent :DMFFlow rate :1.0mL/minDetector :RIColumn temp.:50˚C

Silicon oil Polycarbonate resin Polyvinylpyrrolidone

Column :Shodex GPC K-806M x 2 Eluent :TolueneFlow rate :1.0mL/min Detector :RI Column temp.:45˚C

Column :Shodex GPC KF-806L x 2Eluent :THFFlow rate :1.0mL/min Detector :RI Column temp.:40˚C

Column :Shodex GPC KD-806M x 2Eluent :10mM LiBr in DMF Flow rate :1.0mL/min Detector :RI Column temp.:50˚C

Melamine formaldehyde Phenoxy resin Bionolle® (Polybutylene succinate/adipate)

Column :Shodex GPC KD-802 x 2 Eluent :10mM LiBr in DMFFlow rate :1.0mL/min Detector :RI Column temp.:50˚C

Column :Shodex GPC KD-806M x 2 Eluent :10mM LiBr in DMF Flow rate :1.0mL/min Detector :RI Column temp.:50˚C

Column :Shodex GPC K-806M x 2 + K-801Eluent :ChloroformFlow rate :1.0mL/min Detector :RI Column temp.:40˚C

Sample : Polyvinylpyrrolidone(PVP), 0.2% each (A) PVP(K-30), 400μL(B) PVP(K-60), 500μL(C) PVP(K-90), 500μL

Sample : Silicone oil 0.1%, 200μL

Sample : 30μLMelamine formaldehyde resin 1%

Sample : 250μLPhenoxy resin 0.2%

Sample : Polycarbonate resin 0.1%, 100μL

Sample : 100μLPolybutylene succinate/adipate 0.2%

Silicone oil

CH3

CH3 O C O C

O n

Sta

ndard

analysis

colu

mns

Org

anic

SE

C (G

PC

) colu

mns : G

enera

l Analysis

Calibration curves for KF-800 series using PS standard

Calibration curves for KF-800L (linear type) series using PS standard

Calibration curves for KD-800 series using PEG/PEO standard

42

Org

anic

SE

C (G

PC

) colu

mns : R

apid

Analysis, H

igh

perfo

rman

ce a

nalysis

Sta

ndard

analysis

colu

mns

Organic SEC (GPC) columns : Rapid Analysis, High performance analysisKF-600 and KF-400HQ columns use packing material that can be easily substituted with other solvents.

These eco-friendly SEC columns use about one third of solvent amount compare to standard columns.

Features

• Approximately half of the analysis time compared to standard columns

• The amount of solvent used is reduced to about one third.

• About 1.5 times better separation performance standard columns, higher resolution

• About 4 times better sensitivity than standard columns, supports high sensitivity analysis

• The amount of solvent used is reduced to about one third.

KF-600

KF-400HQ

News No.3, 10

Rapid analysis downsized columns (6.0 mm I.D. x 150 mm)

GPC KF-601

GPC KF-602

GPC KF-602.5

GPC KF-603

GPC KF-604

GPC KF-605

GPC KF-606

GPC KF-606M

GPC KF-607

GPC KF-G

GPC KF-600D

Product Name

F6028091

F6028092

F6028093

F6028094

F6028095

F6028096

F6028097

F6028098

F6028099

F6700300

F6709351


≧ 17,000

≧ 17,000

≧ 17,000

≧ 17,000

≧ 16,000

≧ 7,000

≧ 7,000

≧ 8,000

≧ 5,000

(guard column)


Exclusion Limit(PS)

1,500

5,000

20,000

70,000

400,000

4,000,000

(20,000,000)*

(20,000,000)*

(200,000,000)*

—

—

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

4.6 x 10

6.0 x 50


3

3

3

3

3

10

10

10

18

8

3

Particle Size(μm)

50

150

300

500

1,500

5,000

10,000

10,000

20,000

—

—

MaximumPore Size

(Å)

MaximumPore Size

(Å)

KF-600 series

Shipping solvent : tetrahydrofuran(THF)*( )Estimated value

* See page 50 for the solvent-peak separation columns.* Contact Shodex or our distributors near you for custom columns.

High performance semi-micro columns (4.6 mm I.D. x 250 mm)

GPC KF-401HQ

GPC KF-402HQ

GPC KF-402.5HQ

GPC KF-403HQ

GPC KF-404HQ

GPC KF-405LHQ

GPC KF-406LHQ

GPC KF-G

GPC KF-400D

Product Name

F6028111

F6028112

F6028114

F6028116

F6028118

F6028119

F6028122

F6700300

F6709356


≧ 25,000

≧ 25,000

≧ 25,000

≧ 25,000

≧ 25,000

≧ 10,000

≧ 10,000

(guard column)


Exclusion Limit(PS)

1,500

5,000

20,000

70,000

400,000

4,000,000

(20,000,000)*

—

—

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 250

4.6 x 10

4.6 x 100


3

3

3

3

3

10

10

8

3

Particle Size(μm)

50

150

300

500

1,500

5,000

10,000

—

—

KF-400HQ series

Shipping solvent : tetrahydrofuran(THF)*( )Estimated value

* See page 50 for the solvent-peak separation columns.* Contact Shodex or our distributors near you for custom columns.



102

101.0 2.01.5 3.02.5 4.03.5

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

olys

tyre

ne)

KF-401HQ

KF-402HQ

KF-402.5HQ

KF-403HQ

KF-404HQ

10 15 20 25 min

21mL

5.7mL

6.0mL

0 5

6 8 10 0 5 10 1512 14

8

1

2

3

4

5

6 7 8 9

12 16 20 minmin min

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

olys

tyre

ne)

102

103

104

105

106

107

KF-601

KF-602

KF-602.5

KF-603

KF-604

KF-606M

KF-605

KF-606

1.51.0 2.0 2.5 3.0 3.5 4.0

43

Comparison of standard, rapid analysis type and high performance type

Column :Shodex GPC KF-600 seriesEluent :THFFlow rate :0.5mL/minDetector :UV(254nm)Column temp.:Room temp.

Column :Shodex GPC KF-802.5 x 2 Shodex GPC KF-402.5HQ x 2 Shodex GPC KF-602.5 x 2Eluent :THFFlow rate : (KF-802.5) 1.0mL/min (KF-402.5HQ) 0.3mL/min (KF-602.5) 0.6mL/minDetector : (KF-802.5) RI(conventional type) (KF-402.5HQ, KF-602.5) RI(small cell volume) Column temp.:40˚C

Column :Shodex GPC KF-400HQ series Eluent :THFFlow rate :0.3mL/minDetector :UV(254nm)Column temp.:Room temp.

Styrene-Acrylonitrile copolymer Polyimide Phthalates

Column :Shodex GPC KF-606M x 2 Eluent :10mM LiBr in DMFFlow rate :0.5mL/min Detector :RI(small cell volume) Column temp.:40˚C

Column :Shodex GPC KF-606M x 2Eluent :10mM LiBr in NMPFlow rate :0.5mL/minDetector :RI(small cell volume)Column temp.:60˚C

Column :Shodex GPC KF-401HQ x 2Eluent :THFFlow rate :0.3mL/minDetector :UV(254nm) (small cell volume) Column temp.:40˚C

Sample : Styrene-Acrylonitrile (30:70) copolymer Sample : Polyimide 0.1%, 10μL Sample : 10μL1. Dioctylphthalate 0.1%2. Dibutyllphthalate 0.1%3. Dipropylphthalate 0.1%4. Diethylphthalate 0.1%5. Dimethylphthalate 0.1%6. Propylbenzene 0.2%7. Ethylbenzene 0.2%8. Toluene 0.2%9. Benzene 0.2%

Sample : EPON1001, 0.2%

Reducing the analysis time to less than half of that for the standard column (KF-802.5) requires KF-602.5 which enables high-speed analysis. On the other hand, KF-402.5HQ has a number of theoretical plates 1.5 times larger than that of the standard column, thereby improving resolution especially in the analysis of molecules that have a small to medium molecular weight. Both columns spend solvent amounting to only about one third of the quantity spent by the standard column and are eco-friendly.

Standard type

KF-802.5 x 250μL injection

Semi-micro high performance type

KF-402.5HQ x 210μL injection

Rapid analysis type

KF-602.5 x 210μL injection

Sta

ndard

analysis

colu

mns

Org

anic

SE

C (G

PC

) colu

mns : R

apid

Analysis, H

igh

perfo

rman

ce a

nalysis

Calibration curves for KF-600 series using PS standard

Calibration curves for KF-400HQ series using PS standard

44

Org

anic

SE

C (G

PC

) colu

mns : H

FIP

solve

nt typ

e H

igh

tem

pera

ture

/Ultra

hig

h te

mp

era

ture

analysis S

EC

(GP

C) c

olu

mn

sS

tandard

analysis

colu

mns

Organic SEC (GPC) columns : HFIP solvent typeSEC analysis using hexafluoroisopropanol (HFIP) as eluent enables analyzing engineering plastics, such as polyamides (Nylon) and polyethylene terephthalate, at room temperatures.

Feature

• High performance column for exclusive use with hexafluoroisopropanol (HFIP)

• Rapid analysis, solvent-saving type

HFIP-800 series

HFIP-600 series

Standard Columns (8.0 mm I.D. x 300 mm)

GPC HFIP-803

GPC HFIP-804

GPC HFIP-805

GPC HFIP-806

GPC HFIP-806M

GPC HFIP-807

GPC HFIP-LG

Product Name

F6028530

F6028540

F6028550

F6028560

F6028590

F6028570

F6700500


≧ 12,000

≧ 12,000

≧ 10,000

≧ 10,000

≧ 10,000

≧ 4,000

(guard column)

Exclusion Limit(PMMA)*

30,000

100,000

1,000,000

(10,000,000)**

(10,000,000)**

(100,000,000)**

—

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 50


10

7

10

10

10

18

15

Particle Size(μm)

500

1,500

5,000

10,000

10,000

20,000

—

MaximumPore Size

(Å)

MaximumPore Size

(Å)

MaximumPore Size

(Å)

HFIP-800 series

*PMMA : polymethylmethacrylate**( )Estimated valueShipping solvent : hexafluoroisopropanol (HFIP)


Features

• Supports a wide range of molecular weights.

• Dedicated to SEC analysis at high/ultrahigh temperatures with a maximum usable temperature of 210˚C.• Suitable for the analysis of supermolecule-containing samples

HT-800 series

UT-800 series

Standard Columns

GPC HT-803

GPC HT-804

GPC HT-805

GPC HT-806

GPC HT-806M

GPC HT-807

GPC HT-G

GPC UT-802.5

GPC UT-806M

GPC UT-807

GPC UT-G

GPC AT-806MS

GPC AT-G

Product Name

F6208700

F6208710

F6208720

F6208730

F6208740

F6208770

F6709410

F6208600

F6208610

F6208620

F6709400

F6208390

F6700280


≧ 7,000

≧ 7,000

≧ 7,000

≧ 7,000

≧ 7,000

≧ 4,000

(guard column)

≧ 4,400

≧ 4,400

≧ 3,300

(guard column)

≧ 6,000

(guard column)


Usable Temperature( ˚C)

70,000

400,000

4,000,000

(20,000,000)**

(20,000,000)**

(200,000,000)**

—

20,000

(20,000,000)**

(200,000,000)**

—

(200,000,000)**

—

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 50

8.0 x 300

8.0 x 300

8.0 x 300

8.0 x 50

8.0 x 250

8.0 x 50


13

13

13

13

13

18

13

30

30

30

30

12

15

100 — 150

100 — 150

100 — 150

100 — 150

100 — 150

100 — 150

100 — 150

100 — 210

100 — 210

100 — 210

100 — 210

Ta — 150***

Ta — 150***

Particle Size(μm)

500

1,500

5,000

10,000

10,000

20,000

—

300

10,000

20,000

—

10,000

—

Shipping solvent : toluene*Exclusion limit was measured with THF

**( )Estimated value*** Ta : ambient temperature


High temperature/Ultra high temperature analysis SEC (GPC) columnsThe SEC analysis of insoluble polymers, such as polyethylene (PE) and polypropylene (PP), at high temperatures (100˚C or higher).

Rapid analysis downsized Columns (6.0 mm I.D. x 150 mm)

GPC HFIP-603

GPC HFIP-604

GPC HFIP-605

GPC HFIP-606

GPC HFIP-606M

GPC HFIP-607

GPC HFIP-G

Product Name

F6021030

F6021040

F6021050

F6021060

F6021080

F6021070

F6700511


≧ 12,000

≧ 12,000

≧ 5,000

≧ 5,000

≧ 6,000

≧ 3,000

(guard column)


30,000

100,000

1,000,000

(10,000,000)**

(10,000,000)**

(100,000,000)**

—

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

6.0 x 150

4.6 x 10


3

3

10

10

10

18

8

Particle Size(μm)

500

1,500

5,000

10,000

10,000

20,000

—

HFIP-600 series

*PMMA : polymethylmethacrylate**( )Estimated valueShipping solvent : hexafluoroisopropanol (HFIP)


102

1065 7 8 9 10 11 12

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

MM

A)

HFIP-803

HFIP-804

HFIP-805

HFIP-806M

HFIP-806

12

3

45

6

7

8

9

102

1065 7 8 9 10 11 12 13

103

104

105

106

107

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

olys

tyre

ne)

HT-803

HT-804

HT-805

HT-806

HT-806M

40 min3530252015

1

2

1

2

12

25 min2015 25 min151010

min5 150 10

min5 150 10

min5 15 150 10 20 30 min

1.0 1.5 2.0 2.5 3.0 3.5 4.0

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

MM

A)

102

103

104

105

106

HFIP-603

HFIP-604

HFIP-605 H

FIP-606

HFIP

-606M

45

Polyethylene terephthalate (PET)

Column :Shodex GPC HFIP-800 seriesEluent :HFIPFlow rate :1.0mL/minDetector :RIColumn temp.:Room temp.

Column :Shodex GPC HFIP-600 seriesEluent :5mM CF3COONa in HFIPDetector :RI(small cell volume)Column temp.:40˚C

Column :Shodex GPC HFIP-606M x 2 Eluent :5mM CF3COONa in HFIPFlow rate :0.6mL/minDetector :RI(small cell volume) Column temp.:40˚C

Polyamide (Nylon 6/10 and Nylon 6) polybutylene terephthalate (PBT)

Column :Shodex GPC HFIP-606M x 2Eluent :5mM CF3COONa in HFIPFlow rate :0.6mL/min Detector :RI(small cell volume) Column temp.:40˚C

Column :Shodex GPC HFIP-805 + HFIP-803Eluent :5mM CF3COONa in HFIP Flow rate :1.0mL/min Detector :RI Column temp.:40˚C

Effect of the Particle Size of Material Packed in High Temperature SEC Columns

Column :Shodex GPC HT-800 seriesEluent :THFFlow rate :1.0mL/minDetector :RIColumn temp.:Room temp.

Column :Shodex GPC UT-806M HT-806M, AT-806MS Eluent :0.1% BHT in ODCB Flow rate :1.0mL/min Detector :RI Column temp.:140˚C

Sample : PBT 0.05%, 500μL Sample : Nylon® 6/10 0.2%, 20μL Sample : Nylon® 6 0.2%, 20μL

Sample : 1. Polystyrene (MW : 20,000,000)2. HDPE-A

For the analysis of samples containing macromolecules, GPC UT columns are recommended because of their ability to prevent the breakage of molecular chains. High temperature SEC columns are suitable for the analysis of insoluble polymers, such as polyethylene and polypropylene.

Mw=19,300

Sample : PET 0.2%, 20μL

Mw=29,300 Mw=38,100

Column : UT-806M x 3(Partical size : 30μm)

Column : HT-806M x 2(Partical size : 13μm)

Column : AT-806MS x 2(Partical size : 12μm)

Vo

VoShear degradation

Shear degradationVo

Sta

ndard

analysis

colu

mns

Org

anic

SE

C (G

PC

) colu

mns : H

FIP

solve

nt typ

e H

igh

tem

pera

ture

/Ultra

hig

h te

mp

era

ture

analysis S

EC

(GP

C) c

olu

mns

Calibration curves for HFIP-800 series using polymethylmethacrylate (PMMA)

Calibration curves for HT-800 series using PS

Calibration curves for HFIP-600 series using polymethylmethacrylate (PMMA)

46

Org

anic

SE

C (G

PC

) colu

mns : L

inear c

alib

ratio

n typ

e S

olve

nt-p

eak se

para

tion

colu

mn

s for o

rganic

SE

C (G

PC

)S

tandard

analysis

colu

mns

3,000

3,000

3,000

—

2,000,000

2,000,000

2,000,000

—

6

6

6

6

F6021041

F6021042

F6021043

F6709621

GPC LF-804

GPC LF-604

GPC LF-404

GPC LF-G

Organic SEC (GPC) columns : Linear calibration typeLF series have linear calibration curves over a wide molecular weight range.

Its special packing material has a wide pore size distribution designed for high linearity of calibration curve.

Solvent-peak separation columns for organic SEC(GPC)

Solvent-peak separation columns retard elution of low-molecular-weight monomers, and polymer additives,

that may interfere with the molecular weight distribution determination of polymers or oligomers.

Use this type of column in combination with a linear column.

Features

• Enables analysis over a broad range of molecular weights (1 to 2 million)

• Highly linear calibration curve without inflection points

• Molecular weight distribution can be determined with precision

• Downsized columns (LF-604 and LF-404)

• High-speed column (LF-604) and high-resolution column (LF-404) are also available

LF series

Standard Columns

Column Size(mm)


Exclusion Limit(PS)Product Code

Particle Size(μm)

MaximumPore Size

(Å)


* Contact Shodex or our distributors near you for custom columns. Shipping solvent : tetrahydrofuran(THF)

Notebook No.28, 29

News No.31

8.0 x 300

6.0 x 150

4.6 x 250

4.6 x 10

≧ 17,000

≧ 9,000

≧ 14,000

(guard column)

10

10

3

3

F6709350

F6709450

F6709351

F6709356

KF-805L, 806L, 806M, 807L

K-805L, 806L, 806M, 807L

KF-606M

KF-405LHQ, 406LHQ

THF

Chloroform

THF

THF

GPC KF-800D

GPC K-800D

GPC KF-600D

GPC KF-400D

Solvent-peak separation columns

Column Size(mm)

I.D. x LProduct NameProduct Code Column Combination Shipping SolventParticle Size

(μm)


8.0 x 100

8.0 x 100

6.0 x 50

4.6 x 100



102

65 7 8 9 10 11 12

103

104

105

106

107

108

Elution volume (mL)

Mol

ecul

ar w

eigh

t

1.0 2.01.5 3.0 3.52.5 4.0

Elution volume (mL)

Mol

ecul

ar w

eigh

t (P

olys

tyre

ne)

102

103

104

105

106

107

LF-404

LF-604

LF-804

50 10 15 20 25 30 min

1512 18 21 24 27 30 33 36 39min

50 10 15 20 50 10 15

5 10 15 20 25min

0 5 10 15 20 25min

0 5 10 15 min

0 5 10 min

min min

1

1

1

2

2

2

3

3

3

4

4

4

5

5

5

6

6

6

7

7

7

47

Column :Shodex GPC LF-804 Eluent :THFFlow rate :1.0mL/minDetector :RIColumn temp.:40˚C

Column :Shodex GPC LF-604, LF-404Eluent :THFFlow rate : (LF-604) 0.5mL/min (LF-404) 0.3mL/minDetector :RI(small cell volume) Column temp.:40˚C

Column :Shodex GPC LF-404 x n Eluent :THFFlow rate : 0.3mL/min Detector :RI(small cell volume) Column temp.:40˚C

Polyvinyl formal Styrene-Butyl methacrylate copolymer Phenoxy resin

Column :Shodex GPC LF-804 x 3Eluent :THFFlow rate :1.0mL/minDetector :RIColumn temp.:40˚C

Column :Shodex GPC LF-404 x 2Eluent :THFFlow rate :0.3mL/minDetector :RI(small cell volume) Column temp.:40˚C

Column :Shodex GPC LF-604 x 2 Eluent :DMF Flow rate :0.5mL/min Detector :RI(small cell volume) Column temp.:50˚C

Polylactic acid Polyamide (Nylon6/6) Effects of solvent separation column

Column :Shodex GPC LF-604 x 2 Eluent :ChloroformFlow rate :0.3 mL/minDetector :RI(small cell volume)Column temp.:40˚C

Column :Shodex GPC LF-404 Eluent :5mM CF3COONa in HFIPFlow rate :0.15 mL/minDetector :RI(small cell volume) Column temp.:40˚C

Column : (1) Shodex GPC KF-806L (2) Shodex GPC KF-806L + KF-800DEluent : THF Flow rate: 1.0mL/min Detector : RI

Sample : Phenoxy resin 0.2%, 20μL

Sample : 1. PS Mw : 1,030,0002. PS Mw : 152,0003. PS Mw : 66,0004. PS Mw : 22,0005. PS Mw : 5,0506. PS Mw : 5807. Ethylbenzene

Sample : Polyvinyl formal 0.2%, 50μL Sample : 10μLStyrene-Butyl methacrylate copolymer 0.2%

Sample : Polyvinyl chlorideSample : Polylactic acid 0.25%, 20μL Sample : Nylon® 6/6 0.1%, 20μL

LF-404 x 1

LF-404 x 2

LF-404 x 3

(1)KF-806L

DOP(Dioctyl phthalate)

(2)KF-806L + KF-800D

Sta

ndard

analysis

colu

mns

Org

anic

SE

C (G

PC

) colu

mns : L

inear c

alib

ratio

n typ

e S

olve

nt-p

eak se

para

tion

colu

mn

s for o

rganic

SE

C (G

PC

)

Calibration curve for LF-804 using PS standard Calibration curves for LF-604 and LF-404 using PS standard

Comparison of polystyrene separation withdifferent numbers of LF-404

48

Colu

mns fo

r LC

/MS

and R

apid

analysis o

f dru

gs

Sta

ndard

analysis

colu

mns

Columns for LC/MS and Rapid analysis of drugs

Reversed phase : polymer-based

5

5

5

5

5

4

4

F7622004

F7622005

F6714011

F6710023

F7620009

F7001006

F7001007

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

ODP2 HP-2B

ODP2 HP-2D

ODP2 HPG-2A

Asahipak ODP-50 4B

Asahipak ODP-50 2D

RSpak DE-413S

RSpak DE-213

Column Size(mm) I.D. x LProduct NameProduct Code Shipping SolventParticle Size

(μm)

2.0 x 50

2.0 x 150

2.0 x 10

4.6 x 50

2.0 x 150

4.6 x 50

2.0 x 150


≧ 3,000

≧ 7,000

(guard column)

≧ 2,500

≧ 5,000

≧ 3,000

≧ 8,000

Multi-mode

5

5

10

F7008240

F7008220

F7008160

H2O/CH3CN=40/60

H2O/CH3CN=40/60


RSpak JJ-50 4D

RSpak JJ-50 2D

RSpak NN-414


(μm)

4.6 x 150

2.0 x 150

4.6 x 150


≧ 4,500

≧ 3,500

≧ 6,000

Organic solvent SEC

3

3

3

F6028111

F6028112

F6028114

THF

THF

THF

GPC KF-401HQ

GPC KF-402HQ

GPC KF-402.5HQ


(μm)

4.0 x 250

4.0 x 250

4.0 x 250


≧ 25,000

≧ 25,000

≧ 25,000

Direct analysis of drug or metabolite in biofluid

5

5

5

5

5

5

5

6

6

6

F7622004

F7622005

F6714011

F7600100

F7600110

F7600024

F7600120

F7600130

F7600140

F7600150

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O/CH3CN=55/45

H2O

H2O

H2O

H2O

H2O

H2O

H2O

ODP2 HP-2B

ODP2 HP-2D

ODP2 HPG-2A

MSpak GF-310 4B

MSpak GF-310 4D

MSpak GF-310 4E

MSpak GF-310 2D

MSpak GS-320 4B

MSpak GS-320 4D

MSpak GS-320 2D


(μm)

2.0 x 50

2.0 x 150

2.0 x 10

4.6 x 50

4.6 x 150

4.6 x 250

2.0 x 150

4.6 x 50

4.6 x 150

2.0 x 150


≧ 3,000

≧ 7,000

(guard column)

≧ 3,000

≧ 10,000

≧ 16,000

≧ 5,500

≧ 2,000

≧ 7,000

≧ 4,000

Saccharides analysis (normal phase mode)

5F7630006 H2O/CH3CN=25/75Asahipak NH2P-50 2D


(μm)

2.0 x 150


≧ 3,500

Acetaminophen

Switch valve

Introduce to MS

Acetaminophen

Theophylline

Theophylline

DisopyramideLidocaine

1ppm IRGANOX 1010 m/z=1176 (Negative)

m/z=473.4 (Negative)

m/z=435.4 (Positive)

m/z=448.2 (Positive)

m/z=219.2 (Negative)

m/z=529.2 (Positive)100ppm IRGANOX 1076

10ppm IRGAFOS 168

1ppm TINUVIN 234

2.5ppm BHTdimer

50ppm BHT

Disopyramide

Lidocaine

Carbamazepine

Carbamazepine

Phenobarbital

PhenobarbitalPhenytoin

Phenytoin

Propranolol

Propranolol

Chlorpheniramine

Lidocainem/z 235

m/z 275

m/z 260

m/z 256

m/z 289

m/z 455

m/z 281

Diphenhydramine

Bupivapacaine

Verapamil

Imipramine

Propranolol

Ethenzamide

5 7.5 10 12.5 152.50min

5 7.5 10 12.5 152.50min

5

1

2

3

10 15 200min

5 6 7 8 9 10 11 12 13 14 15 161 2 3 40min

5 6 7 8 9 10 11 12 13 141 2 3 40min

5 6 7 8 9 10 11 12 13 14 151 2 3 40min

5 6 7 8 9 10 11 12 13 14 15 161 2 3 40min

6 8 10

11.77

12.81

13.01

13.50

14.41

15.03

12 14 162 40min

49

LC/MS analysis of drugs in BSA LC/MS analysis of Nonylphenol ethoxylates

Column : Shodex MSpak GF-310 4DEluent : (A); CH3CN, (B): H2O A/B=30/70(7min)→(23min)→A/B=90/10Flow rate: 0.5mL/minDetector : ESI-MS(SIM Positive)

Column :Shodex ODP2 HP-2BEluent :10mM CH3COONH4 aq./CH3CN=70/30Flow rate :0.2mL/minDetector :UV(280nm) ESI-MS(SIM Positive)Column temp.:40˚C

Drugs against Helicobacter pylori

Column :Shodex MSpak GF-310 2DEluent :50mM Ammonium acetate buffer (pH7.0)/CH3CN=70/30 Flow rate :0.1mL/min Detector :EC(Electrode: Glassy carbon, 900mV SCE) Column temp.:45˚C

Column :Shodex MSpak GF-310 4B, GS-320 4BEluent :30mM CH3COONH4 aq./CH3CN=70/30Flow rate :0.25mL/minDetector :UV(220nmn)Column temp.:40˚C

LC/MS analysis of additives (Irganox1010) in polymer

Column :Shodex GPC KF-402HQ x 2Eluent :THFFlow rate :0.3mL/minDetector :UV(210nm), APSI-MS(SIM)Column temp.:40˚C

Sample : Nonylphenol ethoxylates, 20μL

Sample : 10mg/L each , 10μL1. Amoxicillin2. Clarythromycin3. Lansoprazole

Sample : drugs 500ng/mL each in BSA 7.0mg/mL, 5μL

TDM drugs using GF and GS columns

Yoshinari Kobuke, Hyogo Pref. Inst. of Enviro. Sci.,10th Symposium on Environmental chemistry program abstracts, (2001) 542-543

The analysis of the additives that are present in polymer usually includes a pretreatment step. However, our SEC columns can separate the additives from the polymer and introduce only the low-molecular-weight fraction containing the additives into MS, thus enabling easy and highly sensitive analysis without any pretreatment step.

The material packed in the GF/GS columns is highly hydrophilic polyvinylalcohol. Although these columns operate mainly in the SEC mode, they may be used also for the rough separation of chemical substances that have similar molecular weights with the use of the slight hydrophobic interaction the packing material exhibits.

Sample : Cup of instant noodles (styrene foam) 1000mg/L, 5μL

GF-310 4B

GS-320 4B

GF-310 4B

GS-320 4B

GF-310 4B

GS-320 4B

GF-310 4B

GS-320 4B

GS-320 4B

GS-320 4B

GF-310 4B

GF-310 4B

IRGANOX 1010

not detected

m/z=1176(Negative)

UV(210nm)

UV (280nm)

Sta

ndard

analysis

colu

mns

Colu

mns fo

r LC

/MS

and R

apid

analysis o

f dru

gs

Simultaneous analysis of various additives in polystyrene

50

Colu

mns fo

r Colu

mn S

witc

hin

g m

eth

od

Sta

ndard

analysis

colu

mns

30

30

30

30

—

2

5

2

5

1

30

30

30

30

—

F8700000

F8700011

F8700012

F8700013

F8700001

MSpak PK-2A 2p

MSpak PK-2A 5p

MSpak PK-4A 2p

MSpak PK-4A 5p

MSpak HLD

Columns for Column Switching method

Pretreatment columns for column switching enable online concentration of trace substances and online removal of high-molecular-weight foreign substances (e.g., proteins).The use of this type of column is recommended when analyzing chemical substances/metabolites contained in biological fluids.

Features

• Traps both hydrophilic and hydrophobic substances at a high removal rate

• Remove proteins

• Higher protein removal rate than PK columns

• Supports a wider pH range (2 to 12) than ODS-based pretreatment columns

• For trapping cationic chemical substances

PK series

GF/GS series

ODP-51

SP-80

Cartridge columns and holder for column switching method

Column Size(mm)

I.D. x LProduct Name Shipping SolventProduct Code Particle Size

(μm)Pore Size

(Å)

* PK columns are cartridge columns and thus should be installed in a column holder "MSpak HLD" before use.

Base Material : Hydrophilic copolymers containing N-vinyl acetoamide

News No.11

2.0 x 10

2.0 x 10

4.0 x 10

4.0 x 10

(Holder for PK)

H2O

H2O

H2O

H2O

—

* GF/GS columns remove proteins well but are not suitable for trapping hydrophilic substances. Use the PK columns for this purpose.

Pcs/box

—

—

—

—

—

—

—

Octadecyl

Sulfopropyl

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol

Polyvinyl alcohol


F8700015

F7600100

F7600110

F7600120

F7600130

F7600140

F7600150

F6710032

F6118251

MSpak GF-4A

MSpak GF-310 4B

MSpak GF-310 4D

MSpak GF-310 2D

MSpak GS-320 4B

MSpak GS-320 4D

MSpak GS-320 2D

Asahipak ODP-51 4B

MSpak SP-80 4B

Columns for column switching method

Column Size(mm)

I.D. x LProduct Name Base Material Shipping SolventProduct Code Functional

Group

9

5

5

5

6

6

6

5

8

ParticleSize(μm)

400

400

400

400

400

400

400

100

5,000

PoreSize(Å)


4.6 x 10

4.6 x 50

4.6 x 150

2.0 x 150

4.6 x 50

4.6 x 150

2.0 x 150

4.6 x 50

4.6 x 50

H2O

H2O

H2O

H2O

H2O

H2O

H2O

H2O/CH3CN=35/65

50mM Na2SO4 aq.


—

≧ 3,000

≧ 10,000

≧ 5,500

≧ 2,000

≧ 7,000

≧ 4,000

≧ 2,000

≧ 800

Eluent 1

Eluent 2

Pump 1

Pump 2

Proteins etc. in sample

Injector

Pretreatment column

Analytical column

Detector

PK-2A

Res

idua

l BS

A (μ

g)

GF-2A Company-A0

100

200

300

400

500

1st time 150th time

Caffeine Caffeine 0

00

5

10

15

20

50 100 150

260000

270000

280000

290000

300000

50 100 150

Pea

k ar

eaR

eten

tion

time

(min

)

Injection times

Injection times

Reproducibility of the peak area

Reproducibility of retention time (RT)

10 12 14 16 18 min 10 12 14 16 18 min

1 m/z=181ESI (Positive)

m/z=195ESI (Positive)

2 3

System diagram for column switching

(Adsorption)Eluent :10mM Ammonium acetate buffer(pH7.0)Flow rate :0.5mL/min

(Elution)Eluent :10mM Ammonium acetate buffer(pH7.0)/CH3CNFlow rate :0.5mL/minDetector :UV(220nm)Column temp. :Room temp.Switching time:5min

Absolute Recovery of Medical Compounds using PK-2A column

Removal rate of proteins in pretreatment columns

(Pretreatment)Eluent :10mM Ammonium acetate buffer(pH7.0)Flow rate :0.5mL/min Column temp. :Room temp.

(Analysis)Column :Shodex PROTEIN KW-604SEluent :0.1% TFA in (H2O/CH3CN=50/50) Flow rate :0.5mL/min Detector :UV(280nm) Column temp. :Room temp. Switching time:5min

Analysis of caffeine in BSA using column switching method

Sample : 20μLCaffeine 1μg/mL in BSA 200mg/mL

Column :(Pretreatment) Shodex MSpak PK-4A (Analysis) Shodex Asahipak ODP-40 4DEluent :(Pretreatment) 20mM Ammonium acetate buffer(pH5.0) (Analysis) 10mM Ammonium acetate buffer(pH5.0)/CH3CN=90/10Flow rate :1.0mL/minDetector :UV(254nm)Column temp. :Room temp.Switching time :10min

Column :(Pretreatment) Shodex MSpak PK-4A (Analysis) Shodex RSpak DE-213 Eluent :(Pretreatment) 10mM Ammonium acetate buffer(pH4.5) (Analysis) 10mM Ammonium acetate buffer(pH4.5)/CH3CN=90/10Flow rate :(Pretreatment) 1.0mL/min (Analysis) 0.2mL/minColumn temp. :40˚CSwitching time :5min

Analysis of methylxantines using column switching method

Sample : 5μL, Methylxantines 250ng/mL each in BSA 200mg/mL1. Theophylline2. Theobromine 3. Caffeine

PK-2A

GF-2A*

Company-A

*GF-2A: Custom-made column of GF-4A (2.0mmI.D. x 10mm)

99.62%

99.80%

97.68%

Acetaminophen

Caffeine

Barbital

Primidone

Trimethadione

Metharbital

Bromvalerylurea

Ethenzamide

Phenobarbital

Phenacetin

Flumazenil

Warfarin

Bromazepam

Chlormezanone

Pentobarbital

Amobarbital

Acetylpheneturide

Carbamazepine

Clofedanol

4-Hydroxytriazolam

Secobarbital

1-Hydroxymethyltriazolam

Mephobarbital

Glutethimide

Estazolam

Indomethacin

Alprazolam

Oxazepam

Triazolam

Chlordiazepoxide

Phenytoin

Flurazepam

Hypaconitine

Desipramine

Etizolam

Aconitine

Brotizolam

Nitrazepam

Maprotyline

Nortriptyline

Biperiden

Imipramine

Propericiazine

Trihexyphenidyl

Clonazepam

Flunitrazepam

Benzoylaconitine

Benzoylhypaconine

Chlorpheniramine

Propranolol

Mesaconine

Nimetazepam

Amitriptyline

Sildenafil citrate

Hydroxyzine

Fludiazepam

Trimipramine

Chlorpromazine

Haloperidol

Diazepam

Levomepromazine

Bromperidol

Clomipramine

Mianserin

Cloxazolam

Clotiazepam

Promethazine

Haloxazolam

Carpipramine

Perfenazine

Oxazolam

Timiperone

Lofepramine

Zotepine

Thioridazine

Medazepam

Clocapramine

Bromocriptine

Diphenhydramine

115

106

94

91

137

94

94

98

96

108

93

81

102

92

93

93

92

97

91

91

97

90

99

93

101

93

99

97

96

133

99

106

97

109

105

110

97

97

90

86

99

97

90

91

96

97

96

83

111

97

118

90

93

95

99

97

107

77

99

97

96

89

95

92

91

96

92

93

99

86

99

88

65

92

97

91

95

80

93

51

Sample Recovery(%) Sample Recovery

(%)

Column Removal rate of proteins

Sta

ndard

analysis

colu

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Colu

mns fo

r Colu

mn S

witc

hin

g m

eth

od

52

①

②

③

③

③

④

⑤

⑥

⑦

⑧

⑨

⑩

④

③

⑪

⑨

⑫

③

③

①

⑬

⑭

③

③

③

⑮

③

⑯

①

⑰

⑱

⑲

100μmol

10μmol

10μmol

5mg

5mg

20mg

—

15mg

40μmol

30mg

30μmol

—

30mg

50μmol

5mg

70μmol

6-7mg

—

10μmol

10mg

4mg

800μmol

40-60μmol

—

4-5mg

50μmol

4-5mg

20mg

20mg

14mg

—

—

18

18

18

18

18

6

18

18

18

18

18

18

18

18

18

18

18

18

18

18

18

10

18

18

18

18

18

18

18

18

18

18

① 0.1M Sodium acetate buffer + 0.5M NaCl + 0.02% NaN3(pH7.0)② 0.1M Ethyl morpholine-acetic acid buffer + 0.02% NaN3(pH7.0)③ 10mM Sodium phosphate buffer + 0.15M NaCl + 0.02% NaN3(pH7.4)④ 50mM Tris-HCl buffer + 0.15M NaCl + 0.02% NaN3(pH7.0)⑤ 10mM Sodium acetate buffer + 0.02% NaN3(pH6.5)⑥ 50mM Tris-HCl buffer + 0.15M NaCl + 0.5mM MnCl2 + 0.5mM CaCl2 + 0.02% NaN3(pH7.4)⑦ 0.1M Potassium phosphate buffer + 0.02% NaN3(pH5.0)⑧ 50mM Sodium phosphate buffer + 0.02% NaN3(pH7.4)⑨ 50mM Ethyl morpholine-acetic acid buffer + 0.02% NaN3(pH6.0)⑩ 10mM Tris-HCl buffer + 0.02% NaN3(pH8.0)

⑪ 10mM Tris-HCl buffer + 10mM NaCl + 0.02% NaN3(pH7.4)⑫ 50mM Tris-HCl buffer + 0.1M NaCl + 1mM MnCl2 + 1mM CaCl2 + 0.2M Glucose + 0.01% Merthiolate(pH7.2)

⑬ 0.1M Sodium phosphate buffer + 0.5M NaCl + 0.02% NaN3(pH7.0) ⑭ 10mM Sodium phosphate buffer + 0.1M NaCl + 0.02% NaN3(pH7.0)⑮ 20mM Sodium phosphate buffer + 0.02% NaN3(pH6.0)⑯ 0.1M Sodium phosphate buffer + 0.15M NaCl + 0.2M Galactose + 0.02% NaN3(pH7.4)⑰ 0.1M Tris-HCl buffer + 0.15M NaCl + 0.2M N-Acetylglucosamine + 0.02% NaN3(pH7.4)⑱ 10mM Phosphate buffer + 1.0M NaCl(pH7.4)⑲ 50mM Phosphate buffer + 0.15M NaCl(pH6.0)

● Shipping Solvent

Affin

ity Chro

mato

gra

phy c

olu

mns

Sta

ndard

analysis

colu

mns

Affinity Chromatography columnsFeatures

• Rigid polymer-based packing material enables high-speed analysis

• Chemically stable bonding groups (spacers) between ligands and substrates

• Minimizes detachment of ligands, ensuring highly reproducible analysis

AFpak

Standard ColumnsColumn Size(mm) I.D. x L

Shipping Solventsee below table

F7118948

F7118940

F7113000

F7112000

F7118963

F7118960

F7118955

F7118943

F7118942

F7118954

F7118949

F7118947

F7118952

F7113010

F7118945

F7118944

F7113080

F7118962

F7113020

F7118958

F7118946

F7118950

F7113030

F7113070

F7113050

F7118957

F7113040

F7118956

F7118953

F7118959

F7118964

F7111000

Aminobezamidine

Acriflavine

5'AMP

Aprotinin

Avidin


Biotin

Concanavalin A

Cibacron blue

Dextran sulfate

Ethylenediaminediacetic acid

N-acetylglucosamine

Gelatin

Glutathione

Heparin

Iminodiacetic acid

Lentil lectin

Lysine(LCA)

Nicotinamide Adenine Dinucleotide (NAD)

Ovomucoid

Protein A

Aminophenylboronic acid

Procion red

Phosphorylethanolamine

Protein G

Phenylalanine

Protamine

Ricinus communis agglutinin I (RCA-I) lectin

Soybean trypsin inhibitor

Wheat germ agglutinin (WGA)

Choline oxydase, acetylcholine esterase

Glucose oxydase

AFpak AAB - 894

AFpak AAF - 894

AFpak AAM - 894

AFpak AAP - 894

AFpak AAV - 894

AFpak ABA - 894

AFpak ABT - 894

AFpak ACA - 894

AFpak ACB - 894

AFpak ADS - 894

AFpak AED - 894

AFpak AGA - 894

AFpak AGE - 894

AFpak AGT - 894

AFpak AHR - 894

AFpak AIA - 894

AFpak ALC - 894

AFpak ALS - 894

AFpak ANA - 894

AFpak AOV - 894

AFpak APA - 894

AFpak APB - 894

AFpak APD - 894

AFpak APE - 894

AFpak APG - 894

AFpak APH - 894

AFpak APR - 894

AFpak ARC - 894

AFpak AST - 894

AFpak AWG - 894

AFpak ACH - 494

AFpak AGO - 494

Product Name LigandLigand Load / gel

(g)Product Code

Particle Size(μm)

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

8.0 x 50

4.6 x 10

4.6 x 10

53

Application

Capacity

AFpak AAB-894

AFpak AAF-894

AFpak AAM-894

AFpak AAP-894

AFpak AAV-894

AFpak ABA-894

AFpak ABT-894

AFpak ACA-894

AFpak ACB-894

AFpak ADS-894

AFpak AED-894

AFpak AGA-894

AFpak AGE-894

AFpak AGT-894

AFpak AHR-894

AFpak AIA-894

ApplicationProduct Name ApplicationProduct Name

Serine protease, etc

RNA, DNA, vitamins, etc

Serine protease, etc

Biotin, Biotin derivatives

Chiral separation (amino acid), etc

Avidin, etc

Glycoproteins, polysaccharides, etc

Albumin, NAD dependent enzymes, etc

Lipoproteins, blood coagulation factors, etc

Nucleic acids, serum proteins, etc.

Lectins, carbohydrate metabolizing enzymes, etc

Fibronectin, etc

Enzymes related to glutathione, etc

Lipoproteins, blood coagulation factors, etc

Interferon, serum proteins, etc

Glycoproteins, polysaccharides, etc

Plasminogen, Plasminogen activator, RNA, DNA, etc

NAD- dependent dehydrogenase, etc

Tripsin-like protease, etc

IgG, immune complexes, etc

Nucleic acids, catecholamines, etc

NAD, NADP-dependent enzymes, interferon, etc

C-reactive proteins, etc

IgG, immune complexes, etc

Alkalophilic protease, etc

IgM, etc

Glycoproteins, polysaccharides

Tripsin-like protease, etc

Glycoproteins, polysaccharides

Glucose (Enzyme-substrate immobilized column)

AFpak ALC-894

AFpak ALS-894

AFpak ANA-894

AFpak AOV-894

AFpak APA-894

AFpak APB-894

AFpak APD-894

AFpak APE-894

AFpak APG-894

AFpak APH-894

AFpak APR-894

AFpak ARC-894

AFpak AST-894

AFpak AWG-894

AFpak ACH-494

AFpak AGO-494

AFpak AAB-894

AFpak AAF-894

AFpak AAM-894

AFpak AAP-894

AFpak AAV-894

AFpak ABA-894

AFpak ABT-894

AFpak ACA-894

AFpak ACB-894

AFpak ADS-894

AFpak AED-894

AFpak AGA-894

AFpak AGE-894

AFpak AGT-894

AFpak AHR-894

AFpak AIA-894

—

ATP Na 1.8mg/column

Lactic dehydronase 1.5mg/g

Trypsin 3mg/g

Biotin 8 micro-g/column

—

Avidin 9mg/g

—

BSA 20mg/column

LDL 5mg/g

—

Lysozyme 0.6mg

Fibronectin 150mg/g

—

Lysozyme 4mg/column

BSA 70mg/column

AFpak ALC-894

AFpak ALS-894

AFpak ANA-894

AFpak AOV-894

AFpak APA-894

AFpak APB-894

AFpak APD-894

AFpak APE-894

AFpak APG-894

AFpak APH-894

AFpak APR-894

AFpak ARC-894

AFpak AST-894

AFpak AWG-894

AFpak ACH-494

AFpak AGO-494

—

Plasminogen 54micro-g/g

Lactic dehydronase 1.4mg/g

Trypin 100mg/g

IgG Human 13mg/g

Sorbitol 0.2mg/column

BSA 72mg/g

C-reactive protein 0.91mg/g

IgG Humnan 10mg/g

Subtilisin Carlsberg 9mg/g

IgM Human 1.9mg/column

—

Trypin 100mg/g

—

—

—

Sta

ndard

analysis

colu

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Affin

ity Chro

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phy c

olu

mns

NAD-dependent dehydrogenase, ATP-dependent kinase, etc

Choline, acetylcholine (Enzyme-substrate immobilized column)

CapacityProduct Name CapacityProduct Name

Sample :Trypsin(Sigma)

Sample :1. p-Nitrophenyl-α-D-galactoside2. p-Nitrophenyl-α-D-glucoside3. p-Nitrophenyl-α-D-mannoside

Sample :Lipoproteins(Sigma)

Sample : Fibrinogen(Sigma) Sample : L-Lactic dehydrogenase

Ligand : Aminobenzamide

Ligand : Lentil lectin Ligand : NAD(Nicotinamide-adenie dinucleotide)

Ligand : Soybean trypsin inhibitor

Ligand : Concanavalin A

Ligand : Dextran sulfate

Sample : Chymotrypsin(Sigma)

0 10 20 30 min

0 20

1

2

3

40 min

0 20 40 min

0 20 3010 40 min

0 2010 30 min 0 2010 30 min

0 20 40 60 min0 5 10 15 20 min

0 20 40 60 min

Sampleinjection

Adsorption of thetarget substance

Elution of the target substance

target substanceligandspacer

base gel

Cleaning

Washing ElutionAdsorption

54

Principle of affinity chromatography Biotin 4-aminobenzoic acid

Column :Shodex AFpak AAV-894Eluent : (A); 0.01M Sodium acetate buffer + 0.15M NaCl(pH7.0) (B); 0.1M Acetic acid aq./Isopropanol =50/50 Step gradient; (A) to (B) Flow rate :1.0mL/min Detector :UV(260nm) Column temp.:Room temp.

Trypsin p-Nitrophenol-glucoside Lipoproteins in plasma

Column :Shodex AFpak AAB-894 Eluent : (A); 0.1M Sodium acetate buffer + 0.5M NaCl(pH 7.0) (B); 0.1M Sodium acetate buffer + 0.5M NaCl(pH 2.8) Step gradient; (A) to (B)Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Column :Shodex AFpak ACA-894Eluent :0.02M Tris-HCl buffer + 0.25M NaCl(pH7.4)Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Column :Shodex AFpak ADS-894Eluent : (A); 0.05M Sodium phosphate buffer (pH7.4) (B); (A) + 1.0M NaCl Step gradient; (A) to (B)Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Fibrinogen L-Lactic dehydrogenase Chymotrypsin

Column :Shodex AFpak ALC-894 Eluent : (A); 50mM Tris-HCl buffer + 0.15M NaCl + 0.5mM CaCl2 + 0.5mM MnCl2(pH7.4) (B); (A) + 0.1M Methyl mannoside Step gradient; (A) to (B)Flow rate :0.5mL/minDetector :UV(280nm)Column temp.:Room temp.

Column :Shodex AFpak ANA-894Eluent : (A); 10mM Potassium phosphate buffer(pH7.0) (B); (A) + 0.5M KCl Step gradient; (A) to (B)Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Column :Shodex AFpak AST-894Eluent : (A); 0.1M Sodium acetate buffer + 0.5M NaCl(pH7.7) (B); 0.1M Sodium acetate buffer + 0.5M NaCl(pH2.8) Linear gradient; 0 to 20min, (A) 20 to 40min, (A) to (B) after 40min (B) Flow rate :1.0mL/minDetector :UV(280nm)Column temp.:Room temp.

Sample :Biotion 4-aminiobenzoic acid

Ligand : Avidine

Eluent A Eluent B

Eluent A Eluent B

Eluent A

LDL

HDL

VLDL

Eluent B

Eluent A Eluent B

Eluent A Eluent B

Eluent A Eluent B

Eluent A Eluent B

Eluent A

Eluent B

Affin

ity Chro

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Sta

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analysis

colu

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55

Info

rmatio

nC

olu

mn T

rouble

Shootin

g

Column Trouble ShootingSome common causes in chromatogram abnormality

Unstable baseline values

Peak tailing

Peakleading

Broad peak

Ghost peak

No peak

Peak split,shoulders

Selection of column and/or eluent appropriate

Strong sample retention

Void existing at top of column Replace column

Change column and/or eluent

······Main cause ······Frequent problem······Solution

Prepare sample again

Wash flow line of HPLC

Adsorption of impurities in column

Channeling in column Replace column

Leading caused by sample properties (ionic and micellar properties)

Change column and/or eluent

· Wash column· Check column performance· Replace column

· Wash column· Check column performance· Replace column

N ON O

YESYESYES

Problem solved by reducing injection volume

Salt in eluent precipitated

· Adsorption of impurities in column· Deterioration of clumn

Sample overload Reduce injection volume

N ON O

YESYESYES

N ON O

N ON O

N ON O

N ON O

Pressure still high when C disconnected

YESYESYES

YESYESYES

YESYESYES

Pressure still high after washing

N ON OColumnblocked

N ON OInjector blocked

Deterioration of column

N ON O

Column end-fittingblocked Replace column

· Check column performance· Replace column

· Change eluent composition· Wash system before analysisSalting out

· Clean up detector· Replace piping

Detector piping blocked

· Clean up injector· Replace injector

· Wash line filter· Replace line filter

N ON OPump piping blocked

· Replace filter in pump· Clean up pump· Replace pump piping

YESYESYES

YESYESYES

Pressure still high when B disconnected

Pressure under maximum column pressure

Screen eluent and sample through 0.45μm filter

YESYESYES

Pressure still high when A disconnected

YESYESYES

Line filter used

Line filter blocked

YESYESYES

Contamination

Impurities existing in eluent Replace eluent

System trouble· Eluent not flowing· Injector at wrong position (load/inject)· Detector deactivated

· Column equilibration insufficient

Sample adsorption in column Repeat sample injection

Check system

Pressure elevation······Main cause

······Frequent problem

······SolutionSTART

Pump Column

Injector

A B C Detector

Column Trouble Shooting

NEWNEW

56

Colu

mns fo

r Exc

lusive

Use

Sta

ndard

analysis

colu

mns

Columns for Exclusive UseRemoval of surfactants

• Removes surfactant from protein or peptide solutions• Washable with organic solvent

PK, GF-4A

H2O

H2O

—

H2O


F8700000

F8700012

F8700001

F8700015

protein or peptide solutions containing surfactant




MSpak PK-2A 2P

MSpak PK-4A 2P

MSpak HLD

MSpak GF-4A

Product NameProduct Code Application Shipping Solvent

2.0 x 10

4.0 x 10

(holder of PK)

4.6 x 10

News No.12

Gelatin

• Molecular weight distribution determination of gelatin for photo film (PAGI method ver 10)• Mean molecular weight distribution determination of gelatin for photo film (PAGI method ver 10)

GS-620 7G-PSB-804 HQ, SB-805 HQ

H2O/CH3OH=70/30

H2O/CH3OH=70/30

0.02％ NaN3 aq.

0.02％ NaN3 aq.

0.02％ NaN3 aq.


F7600023

F6710019

F6429104

F6429105

F6709430

Molecular weight distribution determination of gelatin for photo film (PAGI method)

Mean molecular weight distribution determination of gelatin for photo film (PAGI method)

Mean molecular weight distribution determination of gelatin for photo film (PAGI method)

Asahipak GS-620 7G-P

Asahipak GS-2G 7B

OHpak SB-804 HQ

OHpak SB-805 HQ

OHpak SB-G


7.5 x 500

7.5 x 50

8.0 x 300

8.0 x 300

6.0 x 50

Taste components

• Multi-mode column mainly used in the SEC mode• For the analysis of nucleic acids in taste components with isocratic elution.

GS-320 7G

H2O/CH3OH=70/30

H2O/CH3OH=70/30


F7610005

F6710019

taste components (nucleic acids)

(guard column)

Asahipak GS-320 7E

Asahipak GS-2G 7B


7.5 x 250

7.5 x 50

Mannitol

• Ca2+-type ligand exchange column• For the analysis of mannitol according to USP (23rd ed.)• Solvents other than water are unnecessary.

MN-431

H2O


F6379230 mannitolUSPpak MN-431


4.0 x 250

Sugar alcohols and Saccharides

• Ca2+-type ligand exchange column• For the analysis of sugar alcohols and saccharides according to European Pharmacopoeia Method (EP)

EP SC1011-7F

H2O


F6379300 sugar alcohols and saccharidesEP SC1011-7F


7.8 x 300

High sensitivity analysis of choline and acethyl-choline

• Reversed phase column with polymer-based packings

• Choline oxydase and acethyl-choline estelaze were introduced• Converts cholines and acetylcholines into hydrogen peroxide.

DE-413

ACH-494

H2O/CH3CN=50/50

H2O/CH3CN=50/50


F7001005

F6700150

F7118964

choline and acethyl-choline

(guard column)

choline and acethyl-choline

RSpak DE-413

RSpak DE-G

AFpak ACH-494


4.6 x 150

4.6 x 10

4.6 x 10 10mM Phosphate buffer (pH7.4)+1.0M NaCl

(guard column)

(guard column)

0 1.0 2.0 3.0 4.0 5.0 min

0 2

2

1

2

3

4

5

6

78

9

1

4

min

6

2

1

3

DE-413

ACH-494

50 10 min

100 20

5 10 15 20 25 30 35 40

30 min

8

0 1.0 2.0 3.0 4.0 5.0 min

BSA+Tween 202

1

1

1

1

1

1

1

1

BSA+Triton X-1003

BSA+SDS4

BSA+CHAPS

GS-620 7G-P x 2

SB-805 HQ x 3 + SB-804 HQ

BSA+Tween 20

BSA+Triton X-100

BSA+SDS

BSA+CHAPS

5

Surfactants are removed by PK-2A

Recovery rate of BSAmore than 97%

KW-604S only KW-604S + PK-2A

Eluent

Pump

Injector

PK-2A

Protein analysis column

Only surfactants are removed

Detector

Sample : 100μlGelatin（Koepff 16922）, 0.2%

Sample : 1. IMP 2. GMP 3. AMP 4. Inosine 5. Hypoxanthine 6. Guanosine 7. Guanine 8. Adenosine 9. Adenine

Eluent

Degas Pump

ECD

Collumn oven

57

Mannitol Choline and Acetylcholine

Effect of surfactants removal by PK-2A

Column : (surfactants removal) Shodex MSpak PK-2A (analysis) Shodex PROTEIN KW-604SEluent :50mM Phosphate buffer + 0.15M NaCl(pH7.0)Flow rate :0.5mL/minDetector :RIColumn temp.:Room temp.

Column :Shodex USPpak MN-431 Eluent :H2OFlow rate :0.5mL/minDetector :RI(small cell volume) Column temp.:60˚C

Column :Shodex RSpak DE-413 Post column :Shodex AFpak ACH-494 Eluent :0.1M Phosphoric acid + 300mg/L Sodium 1-decansulfonate + 65mg/L Telramethylammonium chloride (pH8.0 adjusted by 1M NaOH)Flow rate :1.0mL/minDetector :EC(Electrode: Pt, 350mV SCE)Column temp.:37˚C

Sample : BSA 0.1% + surfactants 0.1%, 50μL1. BSA2. Tween 203. Triton X-1004. SDS5. CHAPS

Sample : 10μL1. Choline 5mg/L2. Ethylhomocholine 10mg/L3. Acetylcholine 10mg/L

Sample : 10μL1. Mannitol 0.5%2. Sorbitol 0.5%

Taste components Gelatin analysis with PAGI method

Column :Shodex Asahipak GS-320 7EEluent :10mM Sodium phosphate buffer(pH5.0)Flow rate :1.0mL/minDetector :UV(260nm)Column temp.:30˚C

Column :Shodex Asahipak GS-620 7G-P x 2 Shodex OHpak SB-805 HQ x 3 + SB-804 HQEluent :0.1M KH2PO4 aq./0.1M Na2HPO4 aq.=50/50Flow rate :1.0mL/minDetector :UV(230nm)Column temp.:50˚C

Online removal of surfactants can be done by just connecting the PK column prior to an analytical column in the same manner as a guard column. The PK columns are cartridge-based columns and thus are easily replaced.

For the analysis of mannitol in conformity to USP (United States Pharmacopoeia 23rd ed.), a column that exhibits a resolution of 2.0 or higher for the combination of mannitol and sorbitol, an internal standard, is required.USPpak MN-431 is a column for mannitol analysis satisfying this requirement.

A multi-mode column GS-320 7E supports the easy and simultaneous analysis of major nucleic acids that are present in taste components, i.e., IMP, GMP, and AMP, and derivatives thereof with isocratic elution.

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Columns for Exclusive UseSurfactants

• SEC columns usable in both water and organic solvents• Excellent solvent compatibility for the analysis of surfactant• Use in quantitative to qualitative analysis

• The packing material contains trace amount of quaternary ammonium groups and supports multimodal (reversed phase and anion exchange) analysis• Suitable for the analysis of anionic surfactants

GF-310 series

JJ-50 series

H2O/CH3OH=70/30

H2O

H2O

H2O/CH3CN=40/60

H2O/CH3CN=40/60

H2O/CH3CN=40/60


F7600001

F7600024

F7600100

F7008240

F7008220

F6650041

Anionic, cationic and nonionic surfactants

and these degradation product

Anionic surfactants like

nonylphenoxycarboxylic acids etc.

Anionic surfactant

Asahipak GF-310 HQ

MSpak GF-310 4E

MSpak GF-310 4B

RSpak JJ-50 4D

RSpak JJ-50 2D

Silica C18M 4E


7.5 x 300

4.6 x 250

4.6 x 50

4.6 x 150

2.0 x 150

4.6 x 250

Notebook No.7

Pesticides

• Reversed phase column with polymer-based packings • Wide usable pH range (pH2~12)• For the analysis of metal coordination compounds such as oxine-copper

GOLF-413CARB-413

H2O/CH3CN=50/50

H2O/CH3CN=50/50

H2O/CH3CN=50/50

H2O/CH3CN=50/50

H2O/CH3CN=50/50


F7009010

F7009040

F7009041

F6700152

F6700150

asulam, oxine-Cu

N-methylcarbamates type pesticids

(line filter for CALB-413, 413L)

(guard column)

RSpak GOLF-413

RSpak CARB-413

RSpak CARB-413L

RSpak CARB-LF

RSpak DE-G


4.6 x 150

4.6 x 150

4.6 x 250

6.0 x 10

4.6 x 10

Oxyhalides

• Supports simultaneous analysis of oxyhalides and common inorganic anions

• High-speed column suitable for postcolumn detection

SI-52 4E

SI-91 4C

3.6mM Na2CO3 aq.




F6995260

F6995280

F6709620

simultaneous analysis of oxyhalides and common inorganic ions.

postcolumn method conforming to the Water Supply Law

(guard column)

IC SI-52 4E

IC SI-91 4C

IC SI-90G


4.0 x 250

4.0 x 100

4.6 x 10

News No.34

News No.34

Cyanide and cyanogen chloride

• For postcolumn detection

• Column for analysis of total cyanide used in combination with an electrochemical detector

KC-811 6E

DEAE-825

0.1% H3PO4 aq.

50mM Na2SO4 aq.


F6378033

F6118255

postcolumn method conforming to the Water Supply Law

total cyanide used in combination with an electrochemical detector

RSpak KC-811 6E

IEC DEAE-825


6.0 x 250

8.0 x 75

News No.34

Arsenic and paraquat

• The packing material containing trace amount of sulfo groups supports multi mode (reversed phase and cation exchange) analysis.

NN series




F7008150

F7008160

morphology of arsenic, paraquat and diquatRSpak NN-614

RSpak NN-414


6.0 x 150

4.6 x 150

（a）

(CH2)11CH3

（CH2CH2O）

1. IO3-

2. CIO2-

3. BrO3-

（b）

DTMAM. Shibukawa. K. Oguma. et al., J. Chromatogr. A. 830. 321（1999）

DTMA+

CTMA+

TDDBA+

TMSA+

CTMA

TDDBA

TMSA

H3C

C9H19 CH2COOHO n

12

345

678

9

10

1

23

4

5

6

7

8 9

CH3

CH3

N+

(CH2)15CH3H3C

CH3

CH3

N+

(CH2)13CH3

CH3

CH3

N+

(CH2)17CH3H3C

CH3

CH3

N+

0 2 4 6 8

20 40 60 80 min

0

12

3

45

6

1

2

3

4

1

2

3

4

5 6

100 200 500 600 700 800 900 1000

1000

2000

12001100300 400

100 200 500 600 700 800 900 1000 12001100300 400

min3.0

1

2

3

3

Standard arsenic compounds

Urine from a healthy personUrine from an arsenic poisoning patient

5.04.02.01.00.0

0 10 20

0

1

2 3

5

7

6

8

10

9

11

4

10 15 20 25 305min

30 min

10 12 min

Sample : �0.1ppm each, 20μL1. Oxamyl2. Methiocarb sulfoxide3. Aldicarb4. Methiocarb sulfone5. Bendiocarb7. Carbaryl8. Fenobucarb9. Methiocarb

0 100 200 500 600 700 800 900 1000

1000

2000

1000

2000

12001100300 400

mineral water (added IO3-, CIO2

-, BrO3- 10μg/L )

BrO3- standard (10μg/L)

2 4 6 8 10 min 2 4 6 8 10 min

CN

CNCl

59

Sample : 50μL 1. F- 2mg/L 2. ClO2

- 1mg/L 3. BrO3

- 1mg/L 4. Cl- 10mg/L 5. NO2

- 5mg/L 6. Br- 10mg/L 7. ClO3

- 1mg/L 8. Dichloroacetate 1mg/L 9. NO3

- 30mg/L10. PO4

3- 15mg/L11. SO4

2- 40mg/L

Sample : 50μL (a) 5μL each (b) 0.5μL each

Sample : 5μLMixed Nonylphenoxy carboxylic acids 1mg/L1. Nonylphenoxy acetic acid2. Nonylphenoxylic carboxylic acids (n=1)3. (n=2), 4. (n=3), 5. (n=4), 6. (n=5)7. (n=6), 8. (n=7), 9. (n=7), 10. (n=9)

Sample : 200μL

Sample : 10μg/L each, 100μL

Oxyhalides analysis with post-column method Oxyhalides analysis with suppressor method

Column :Shodex IC SI-91 4CEluent :1.8mM Na2CO3+1.7mM NaHCO3 aq.Reagent : (A); 1.0M KBr+1.5M H2SO4 aq. (B); 1.2mM NaNO2 aq.Flow rate : (Eluent) 1.0mL/min (Reagent A) 0.4mL/min (Reagent B) 0.2mL/minDetector :UV(268nm)Column temp.:40˚C

Column :Shodex RSpak KC-811 6EEluent :1.0mM H2SO4 aq.Reagent A :Chloramine T solutionReagent B :4-Pyridinecarboxylic acid-Pyrazolone solutionFlow rate :(Eluent) 1.0mL/min (Reagent) 0.5mL/min eachDetector :VIS(638nm) Column temp. :40˚CReaction temp.:(Reagent A) 40˚C (Reagent B) 80˚C

Column :Shodex IC SI-52 4EEluent :3.6mM Na2CO3 aq.Flow rate : 1.0mL/minDetector :Suppressed conductivityColumn temp.:45˚C

IC SI-52 4E is a high-performance column whose number of theoretical plates is at least 2.5 times larger than what the standard column SI-90 4E has. The analysis of halogen oxides and typical anions can be performed in the suppressor method, in which electric conductivity is used as a separation criteria.

Cationic surfactants Anionic surfactants N-methylcarbamate pesticides

Column :Shodex Asahipak GF-310 HQ*Eluent :0.4mM 4,4'-Dipyridil-0.8mM HCl (CH3CN 27%)Flow rate :0.6mL/minDetector :Suppressed conductivityColumn temp.:40˚C

*special size (4.6mmI.D. x 100mm)

Column :Shodex RSpak JJ-50 4D Eluent : (A); 25mM Ammonium acetate buffer(pH9.5)/CH3CN=85/15 (B); 25mM Ammonium acetate buffer(pH9.5)/CH3CN=70/30 Linear gradient: (A) to (B), 75minFlow rate :0.6mL/min Detector :Fluorescence(Ex: 225nm Em: 300nm) Column temp.:40˚C

Column :Shodex RSpak CARB-413Eluent :CH3CN/H2O=37/63Flow rate :0.8mL/minReagent : (A); 50mM NaOH (B); 0.05% o-Phthalaldehyde + 0.1% MercaptoethanolDetector :Fluorescence(Ex: 340nm, Em: 450nm)Column temp.:40˚C

Sample : Arsenic compounds, 50μL1. Monomethylarsonic acid, MMAA2. Arsinic acid, As(III)3. Dimethylarsinic acid, DMAA4. Arsenobetaine, AB5. Tetramethylarsonium, TeMA6. Trimethylarsine oxide, TMAO

Cyan and cyanogen chloride with post-column method Morphology of arsenic compounds

Column :Shodex RSpak NN-614 Eluent :5mM HNO3/8mM NH4NO3 aq.Flow rate :0.8mL/minDetector : IC-ICP-MS(SIM m/z=75)

Noriko TSUNODA, National Reseach Institute of Police Science, Pharmacia, vol.34 No.12 (1998), p1237-12421

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16

16

16

16

16

30

30

(guard column)

30

(guard column)

F6090005

F6090001

F6090002

F6090003

F6090004

CLNpak EV-200

CLNpak EV-2000

CLNpak EV-G

CLNpak EV-2000 AC

CLNpak EV-G AC

Columns for Sample Clean-up

The CLNpak series are pretreatment columns packed with rigid polymer-based gel.

Target sample is cleaned up from a complex matrix in size exclusion (SEC) mode.

Features

• For the separation of residual pesticides in food

• Adopted as the purification method in test solution preparation in Notification No. 1003001 of the Dept. of

Food Safety, MHLW, Section 2 "Simultaneous GC/MS (LC/MS) Analyses of Agricultural Chemicals in

Livestock and Marine Products"

• For the clean-up of samples in water-rich matrix such as blood and bottom sediment

• Highly effective in the separation of endocrine disruptors in environmental samples

EV series

PAE series

GPC Clean-up for residual pesticides in food

Column Size(mm)


(μm)Pore Size

(Å)

* Contact Shodex or our distributors near you for custom columns. Base Material : Styrene divinylbenzene copolymer

2.0 x 150

20.0 x 300

20.0 x 100

20.0 x 300

20.0 x 100

Ethylacetae/Cyclohexane=3/7



Acetone/Cyclohexane=3/7

Acetone/Cyclohexane=3/7

5

5

9

5

5

9

400

400

(guard column)

400

400

(guard column)

F7600025

F6810022

F6714007

F7600026

F6810023

F6714008

CLNpak PAE-800

CLNpak PAE-2000

CLNpak PAE-G

CLNpak PAE-800 AC

CLNpak PAE-2000 AC

CLNpak PAE-G AC

GPC Clean-up for phthalic acid esters in sediments, biological samples and blood.

Column Size(mm)


(μm)Pore Size

(Å)

* Contact Shodex or our distributors near you for custom columns. Base Material : Polyvinyl alcohol

8.0 x 300

20.0 x 300

8.0 x 50

8.0 x 300

20.0 x 300

8.0 x 50

Acetonitrile

Acetonitrile

Acetonitrile

Acetone

Acetone

Acetone

*1 purification with Ethylenediamine-N-propylsilyled silica-based gel mini-column modified *2 purification with silica gel mini-column

liver, kidney

acetone/n-hexane (1:2)

Ext

ract

ion

Pur

ifica

tion

fraction-1

[*1] [*1] [*1]

[*2]

fraction-2 pesticidefraction

pesticidefraction

GPC

test sample for GC/MS

GC/MS、 LC/MS

GPC

acetonitrile

salting-out

muscle, fat, fish milk, egg honey

Acrinathrin (5mg/L) (marker pesticide)

pesticidefraction

fraction-2

fraction-1

muscle, fat, fish, milk, egg

liver, kidney

Tricyclazole (5mg/L) (marker pesticide)

0 5 10 15 20 25 30 35 min

Acrinathrin (5mg/L)

GPC Clean-ups of Fatty Tuna

Tricyclazole (5mg/L)

GPC Clean-ups of Beef Tallow

→ →

0 5 10 15 20 25 30 35 min

former elution fraction

Acrinathrin

Bisphenol A

Courtesy of Prof. Kazuichi Hayakawa, Faculty of Pharmaceutical Sciences, Kanazawa University.

Benzo[a]fluoranthene

Chinomethionate

Tricyclazole

current elution fraction

Sample :

40mg/L each

RI Corn oil Sulfer

Di-2-ethylhexyl adipate

Phthalic acid esters

4-Nonylphenol

DDT

Bisphenol A

Anthracene

H2O

CO2

RI

RI

RI

RI

UV(330nm)

UV(330nm)

0 15 20 25

min

min

30

10 20

14 15 16 17 18 19 20 min

56

Time

Volume 60 64 68 72 76 80 mL

30

35

Fluvalinate

1,3-Dinitropyrene

Benzo[a]pyrene

Hydroxybenzo[a]pyrene mixture PCBs

C19 C12

PCBs

Compound Rt(min) Compound Rt(min)

AnthraceneChryseneFluorantheneBenzo[a]flucrantheneBenzo[b]flucrantheneBenzo[k]flucranthenePyrene1-Hydroxypyrene

16.618.017.117.718.618.417.919.7

19.923.0

16.116.218.618.618.918.7

Benzo[a]pyreneHydroxybenzo[a]pyrene compoundBisphenol A1,3-Dinitronaphthalene1-Nitropyrene1,3-Dinitropyrene1,6-Dinitropyrene1,8-Dinitropyrene

Chlordane, Toxaphene

Polychlorinated naphthalene

Polybrominated biphenylether

Aliphatic hydrocarbonMethylanthracene

Anthracene

Source: “Studies on the Pre-treatment of Polychlorinated Biphenyls (PCBs) Analysis in Waste Oil,”Koichi SASAKI et al., analytical Center, Kokan Keisoku Corp., 9th Symposium on Environmental Chemistry Program and Abstracts (2000), pp. 240-241

Elution Positions of Phthalic Acid Esters measured using PAE-800 AC

GPC Clean-ups of Carcinogens in Diesel Dust measured using PAE-800 AC Pretreatment of PCBs in Waste Oil

Column :Shodex CLNpak PAE-800 ACEluent :AcetoneFlow rate :0.5mL/minDetector :UV(330nm), RIColumn temp.:Room temp.

Column :Shodex CLNpak PAE-800 ACEluent :Acetone Flow rate :0.8mL/min Detector :UV(210nm) Column temp.:Room temp.

Column : Shodex CLNpak PAE-G AC + PAE-2000 ACEluent : Acetone Flow rate : 4.0mL/min Detector : UV(213nm)

Preparation of Test Solutions Used in Simultaneous GC/MS and LC/MS Analyses of Agricultural Chemicals in Livestock and Marine Products (Outline) GPC Clean-up of Pesticide Residues using PAE-800 AC

Column :Shodex CLNpak EV-G AC + EV-2000 AC Eluent :Acetone/Cyclohexane=1/4Flow rate :5mL/minDetector :UV(254nm)Column temp.:40˚CInjection vol. :5mL

GPC Clean-ups of Fatty Tuna and Beef Tallow Fractionation Standards



[Extraction]

1) Weigh 20.0 mg of the sample (5.0g for fat). 2) Add 20 mL of water and homogenize. 3) Add 100 mL of acetone/n-hexane (1/2)

and homogenize. 4) Centrifuge (2,500 rpm, 5 min.) and collect

the organic layer (1). 5) Add 50 mL of n-hexane to the residue

and homogenize. 6) Centrifuge (2,500 rpm, 5 min.) and collect

the organic layer (2). 7) Combine (1) and (2) and add anhydrous

sodium sulfate for dehydration. 8) Remove anhydrous sodium sulfate by

filtering and concentrate the filtrate at 40˚C or lower.

9) Remove the solvent.10) Weigh the residue (extracted fat).11) Dissolve in acetone/cyclohexane (1/4).

61

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No. Packing material Recommended Column Page

Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.5 to 10μm in diameter, or a monolithic rod.

Octylsilane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameter.

An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3 to 10μm in diameter.

Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11μm in diameter.

Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9μm in diameter.

Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10μm in diameter.

A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10μm in diameter.

A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10μm in size.

An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quartenary ammonium groups, about 10μm in size.

Butyl silane chemically bonded to totally porous silica particles, 3 to 10μm in diameter.

Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 daltons. It is spherical, silica-based, and processed to provide pH stability.

Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, about 9μm in diameter.

Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 daltons. It is a polymethacrylate gel.

A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.

Beta cyclodextrin bonded to porous silica particles, 5 to 10μm in diameter.

Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 7 to 11μm diameter.

Packing having the capacity to separate proteins by molecular weight over the range of 10 to 500 kDa. It is spherical (10μm), silica-based, and processed to prodive hydrophilic characteristics and pH stability.

A methacrylate-based size-exclusion packing for water-soluble samples.

Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable.

Trimethylsilane chemically bonded to porous silica particles, 3 to 10μm in diameter.

Phenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameter.

Nitrile groups chemically bonded to porous silica particles, 3 to 10μm in diameter.

Porous silica particles, 5 to 10μm in diameter.

PROTEIN KW-803

Silica C18M

Silica C18P

ODSpak F-411

Silica 5SIL

Silica 5C8

Silica 5NH

Silica 5CN

Silica 5NPE

Silica 5TMS

SUGAR SH1011 andRSpak KC-811

24, 28,58

SUGAR SC1011

USPpak MN-431

PROTEIN KW-800 series

RSpak DS-613

RSpak RP18-415

SUGAR SH1011

RSpak DC-613

RSpak KC-811

SUGAR SP0810

SUGAR SC1011

SUGAR KS-800 series

USPpak MN-431

IEC QA-825

OHpak SB-802 HQ

Silica 5C4

PROTEIN KW-802.5

PROTEIN KW-803

SUGAR SP0810

OHpak SB-803 HQ

OHpak SB-800 HQ series

OHpak SB-800 HQ series

RSpak DM-614

ORpak CDBS-453

32

12, 58

12

12

12

12

12

12

12

12

24

24, 56

32

8

8

8

GPC, KF, K, KD, LF series 40, 46

24

10

28, 58

24

24

24

24, 56

16

34

24

12

34

34

34

34

34

8

33

246-17μm SUGAR KS-800 series may be suited

L 1

L 3

L 7

L 8

L 10

L 11

L 13

L 17

L 19

L 20

L 21

L 22

L 23

L 25

L 26

L 33

L 34

L 37

L 38

L 39

L 45

L 58

L 59

(3.5μm RSpak DS-413 and3.5μm RSpak RP18-413may be suited)

US

P C

olu

mn L

istIn

form

atio

n

USP Column List

63

Version dateReferenceNumber Monograph

Recommended column

Product name Product code Page

Info

rmatio

nE

P C

olu

mn L

ist

EP Column List

Calcium Lactate Monohydrate

Calcium Lactate Pentahydrate

Calcium Lactate Trihydrate

Calcium Lactate, Anhydrous

Diethylene Glycol Palmitostearate

Enalapril Maleate

Erythritol

Ethylene Glycol Monopalmitostearate

Fish Oil, Rich in Omega-3-acids

Formoterol Fumarate Dihydrate

Glycerol Dibehenate

Glycerol Distearate

Glycerol Monolinoleate

Glycerol Mono-Oleate

Glycerol Monostearate 40-55

Haemofitration and For Haemodiafiltratin, Solutions For

Heparins, Low-Molecular-Mass

Human Albumin Solution

Human Normal Immunoglobulin

Human Normal Immunoglobulin for Intravenous Administration

Human Plasma (Pooled and treated for Virus Inactivation)

Insulin Aspart

Insulin Lispro

Insulin Preparations, Injectable

Insulin, Bovine

Insulin, Human

Insulin, Porcine

Interferon Gamma-1b Concentrated Solution

Interferon Gamma-1b Concentrated Solution

Isomalt

Lactitol Monohydrate

Macrogol 15 Hydroxystearate

Malic Acid

Maltitol, Liquid

Mannitol

myo-Inositol

Olsalazine Sodium

Omega-3-acid Ethyl Esters 60




Omega-3-acid Triglycerides

Omega-3-acid Triglycerides

Pefloxacin Mesilate Dihydrate

Peritoneal Dialysis, Solutions For

Propylane Glycol Monopalmitostearate

Propylene Glycol Dilaurate

Propylene Glycol Monolaurate

Sorbitol

Sorbitol, Liquid (Crystallising)

Sorbitol, Liquid (Non-Crystallising)

Sorbitol, Liquid, Partially Dehydrated

Somatropin

Somatropin Bulk Solution

Somatropin for Injection

24, 56

8

24, 56

24, 56

40

8

24, 56

40

40

6

40

40

40

40

40

24, 56

32

32

32

32

24, 56

32

32

32

32

32

32

32

14

24, 56

24, 56

32

28

24, 56

24, 56

24, 56

28

40

40

40

40

40

40

6

24, 56

40

40

40

24, 56

24, 56

24, 56

24, 56

32

32

32

EP SC1011-7F

EP SC1011-7F

EP SC1011-7F

EP SC1011-7F

KF-802

DS-413

EP SC1011-7F

KF-802

KF-802

ODP-50 4D

KF-802

KF-802

KF-802

KF-802

KF-802

EP SC1011-7F

KW-802.5

KW-803

KW-803

KW-803

EP SC1011-7F

KW-802.5

KW-802.5

KW-802.5

KW-802.5

KW-802.5

KW-802.5

KW-803

SP-825

EP SC1011-7F

EP SC1011-7F

KW-802.5

KC-811

EP SC1011-7F

EP SC1011-7F

EP SC1011-7F

KC-811

KF-802

KF-803

KF-802

KF-803

KF-802

KF-803

ODP-50 6D

EP SC1011-7F

KF-802

KF-802

KF-802

EP SC1011-7F

EP SC1011-7F

EP SC1011-7F

EP SC1011-7F

KW-802.5

KW-802.5

KW-802.5

F6379300

F6379300

F6379300

F6379300

F6028020

F7001012

F6379300

F6028020

F6028020

F7620004

F6028020

F6028020

F6028020

F6028020

F6028020

F6379300

F6989000

F6989103

F6989103

F6989103

F6379300

F6989000

F6989000

F6989000

F6989000

F6989000

F6989000

F6989103

F6118250

F6379300

F6379300

F6989000

F6378030

F6379300

F6379300

F6379300

F6378030

F6028020

F6028030

F6028020

F6028030

F6028020

F6028030

F7620002

F6379300

F6028020

F6028020

F6028020

F6379300

F6379300

F6379300

F6379300

F6989000

F6989000

F6989000

04/2005

04/2005

04/2005

04/2005

07/2005

01/2005

01/2005

01/2005

01/2005

07/2006

04/2005

04/2007

01/2005

01/2007

04/2007

01/2005

01/2005

01/2006

01/2006

01/2006

01/2007

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

07/2006

01/2007

07/2007

01/2005

01/2005

01/2005

01/2006

01/2006

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2005

01/2006

01/2006

01/2006

2117

0468

0469

2118

1415

1420

1803

1421

1912

1724

1427

1428

1429

1430

0495

0861

0828

0255

0338

0918

1646

2084

2085

0854

1637

0838

1638

1440

1440

1531

1337

2052

2080

1236

0559

1805

1457

2063

2063

1250

1250

1352

1352

1460

0862

1469

2087

1915

0435

0436

0437

2048

0951

0950

0952

EP : European Pharmacopoeia Method

Semi-micro and micro columns

64

Polymer-based Packed Columns for Reversed and Normal Phase Chromatography

ODP40-2E

ODP40-2D

ODP40-2B

ODP40-2T

ODP40-1E

ODP40-1D

ODP40-1B

ODP40-1T

ODP40-M8E

ODP40-M8D

ODP40-M8B

ODP40-M8T

ODP40-M5E

ODP40-M5D

ODP40-M5B

ODP40-M5T

ODP40-M3E

ODP40-M3D

ODP40-M3B

ODP40-M3T

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product Name Length(mm)I.D.

250

150

50

35

250

150

50

35

250

150

50

35

250

150

50

35

250

150

50

35

F7838021

F7838022

F7838023

F7838024

F7838031

F7838032

F7838033

F7838034

F7838041

F7838042

F7838043

F7838044

F7838051

F7838052

F7838053

F7838054

F7838061

F7838062

F7838063

F7838064

Product Code

NN414-2D

NN414-2B

NN414-2T

NN414-1D

NN414-1B

NN414-1T

NN414-M8D

NN414-M8B

NN414-M8T

NN414-M5D

NN414-M5B

NN414-M5T

NN414-M3D

NN414-M3B

NN414-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7860122

F7860123

F7860124

F7860132

F7860133

F7860134

F7860142

F7860143

F7860144

F7860152

F7860153

F7860154

F7860162

F7860163

F7860164

Product Code

* See page 6 for Asahipak ODP-40 series.* See page 76 for the preparative columns.

* See page 8 for RSpak DE-413 series.* See page 76 for the preparative columns.

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 8 for RSpak NN-414.

Asahipak ODP-40 semi-micro and micro type

NH2P40-2E

NH2P40-2D

NH2P40-2B

NH2P40-2T

NH2P40-1E

NH2P40-1D

NH2P40-1B

NH2P40-1T

NH2P40-M8E

NH2P40-M8D

NH2P40-M8B

NH2P40-M8T

NH2P40-M5E

NH2P40-M5D

NH2P40-M5B

NH2P40-M5T

NH2P40-M3E

NH2P40-M3D

NH2P40-M3B

NH2P40-M3T

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm


250

150

50

35

250

150

50

35

250

150

50

35

250

150

50

35

250

150

50

35

F7858021

F7858022

F7858023

F7858024

F7858031

F7858032

F7805033

F7805034

F7858041

F7858042

F7858043

F7858044

F7858051

F7858052

F7858053

F7858054

F7858061

F7858062

F7858063

F7858064

Product Code

* See page 10 for Asahipak NH2P-50 series.* See page 77 for the preparative columns.

Asahipak NH2P semi-micro and micro typeRSpak NN-414 semi-micro and micro type

DE413-2E

DE413-2D

DE413-2B

DE413-2T

DE413-1E

DE413-1D

DE413-1B

DE413-1T

DE413-M8D

DE413-M8B

DE413-M8T

DE413-M5D

DE413-M5B

DE413-M5T

DE413-M3D

DE413-M3B

DE413-M3T

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm


250

150

50

35

250

150

50

35

150

50

35

150

50

35

150

50

35

F7840121

F7840122

F7840123

F7840124

F7840131

F7840132

F7840133

F7840134

F7840142

F7840143

F7840144

F7840152

F7840153

F7840154

F7840162

F7840163

F7840164

Product Code

RSpak DE-413 semi-micro and micro type

Polym

er-b

ase

d P

acked C

olu

mns fo

r Reve

rsed

an

d N

orm

al P

hase

Ch

rom

ato

gra

ph

yS

em

i-mic

ro a

nd

mic

ro c

olu

mn

s

65

Cation Exchange Chromatography

SP8-2D

SP8-2B

SP8-2T

SP8-1D

SP8-1B

SP8-1T

SP8-M8D

SP8-M8B

SP8-M8T

SP8-M5D

SP8-M5B

SP8-M5T

SP8-M3D

SP8-M3B

SP8-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7940122

F7940123

F7940124

F7940132

F7940133

F7940134

F7940142

F7940143

F7940144

F7940152

F7940153

F7940154

F7940162

F7940163

F7940164

Product Code

SP3N-2B

SP3N-2T

SP3N-1B

SP3N-1T

SP3N-M8B

SP3N-M8T

SP3N-M5B

SP3N-M5T

SP3N-M3B

SP3N-M3T


50

35

50

35

50

35

50

35

50

35

F7950123

F7950124

F7950133

F7950134

F7950143

F7950144

F7950153

F7950154

F7950163

F7950164

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 14 for IEC SP-825.* See page 78 for the preparative columns.

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 14 for IEC SP-420N.

CM8-2D

CM8-2B

CM8-2T

CM8-1D

CM8-1B

CM8-1T

CM8-M8D

CM8-M8B

CM8-M8T

CM8-M5D

CM8-M5B

CM8-M5T

CM8-M3D

CM8-M3B

CM8-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7940222

F7940223

F7940224

F7940232

F7940233

F7940234

F7940242

F7940243

F7940244

F7940252

F7940253

F7940254

F7940262

F7940263

F7940264

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 14 for IEC CM-825.* See page 78 for the preparative columns.

IEC CM-825 semi-micro and micro type

CM9A-2D

CM9A-2B

CM9A-2T

CM9A-1D

CM9A-1B

CM9A-1T

CM9A-M8D

CM9A-M8B

CM9A-M8T

CM9A-M5D

CM9A-M5B

CM9A-M5T

CM9A-M3D

CM9A-M3B

CM9A-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7960222

F7960223

F7960224

F7960232

F7960233

F7960234

F7960242

F7960243

F7960244

F7960252

F7960253

F7960254

F7960262

F7960263

F7960264

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 14 for Asahipak ES-502C 7C.* See page 78 for the preparative columns.

Asahipak ES-502C 7C semi-micro and micro type

IEC SP-825 semi-micro and micro type IEC SP-420N semi-micro and micro type

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

sC

atio

n E

xchange C

hro

mato

gra

phy

66

* See page 16 for IEC DEAE-825.* See page 78 for the preparative columns.

Anion Exchange Chromatography

QA8-2D

QA8-2B

QA8-2T

QA8-1D

QA8-1B

QA8-1T

QA8-M8D

QA8-M8B

QA8-M8T

QA8-M5D

QA8-M5B

QA8-M5T

QA8-M3D

QA8-M3B

QA8-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7940322

F7940323

F7940324

F7940332

F7940333

F7940334

F7940342

F7940343

F7940344

F7940352

F7940353

F7940354

F7940362

F7940363

F7940364

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

DEAE8-2D

DEAE8-2B

DEAE8-2T

DEAE8-1D

DEAE8-1B

DEAE8-1T

DEAE8-M8D

DEAE8-M8B

DEAE8-M8T

DEAE8-M5D

DEAE8-M5B

DEAE8-M5T

DEAE8-M3D

DEAE8-M3B

DEAE8-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7940422

F7940423

F7940424

F7940432

F7940433

F7940434

F7940442

F7940443

F7940444

F7940452

F7940453

F7940454

F7940462

F7940463

F7940464

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 16 for IEC QA-825.* See page 78 for the preparative columns.

IEC QA-825 semi-micro and micro type IEC DEAE-825 semi-micro and micro type

* See page 16 for Asahipak ES-502N 7C.* See page 78 for the preparative columns.

DEAE9A-2D

DEAE9A-2B

DEAE9A-2T

DEAE9A-1D

DEAE9A-1B

DEAE9A-1T

DEAE9A-M8D

DEAE9A-M8B

DEAE9A-M8T

DEAE9A-M5D

DEAE9A-M5B

DEAE9A-M5T

DEAE9A-M3D

DEAE9A-M3B

DEAE9A-M3T


150

50

35

150

50

35

150

50

35

150

50

35

150

50

35

F7960122

F7960123

F7960124

F7960132

F7960133

F7960134

F7960142

F7960143

F7960144

F7960152

F7960153

F7960154

F7960162

F7960163

F7960164

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Asahipak ES-502N 7C semi-micro and micro type

DEAE3N-2B

DEAE3N-2T

DEAE3N-1B

DEAE3N-1T

DEAE3N-M8B

DEAE3N-M8T

DEAE3N-M5B

DEAE3N-M5T

DEAE3N-M3B

DEAE3N-M3T


50

35

50

35

50

35

50

35

50

35

F7950223

F7950224

F7950233

F7950234

F7950243

F7950244

F7950253

F7950254

F7950263

F7950264

Product Code

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 16 for IEC DEAE3N-4T.

IEC DEAE3N-4T semi-micro and micro type

Anio

n E

xchange C

hro

mato

gra

phy

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

s

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 32 for KW402.5-4F.

67

Aqueous SEC(GFC) column ; Silica-based

KW402.5-4D

KW402.5-4B

KW402.5-2E

KW402.5-2D

KW402.5-2B

KW402.5-1E

KW402.5-1D

KW402.5-1B

KW402.5-M8E

KW402.5-M8D

KW402.5-M8B

KW402.5-M5E

KW402.5-M5D

KW402.5-M5B

KW402.5-M3E

KW402.5-M3D

KW402.5-M3B


150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7781212

F7781213

F7781221

F7781222

F7781223

F7781231

F7781232

F7781233

F7781241

F7781242

F7781243

F7781251

F7781252

F7781253

F7781261

F7781262

F7781263

Product Code

KW402.5-4F semi-micro and micro type

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm

* See page 32 for KW403-4F.

KW403-4D

KW403-4B

KW403-2E

KW403-2D

KW403-2B

KW403-1E

KW403-1D

KW403-1B

KW403-M8E

KW403-M8D

KW403-M8B

KW403-M5E

KW403-M5D

KW403-M5B

KW403-M3E

KW403-M3D

KW403-M3B


150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7781312

F7781313

F7781321

F7781322

F7781323

F7781331

F7781332

F7781333

F7781341

F7781342

F7781343

F7781351

F7781352

F7781353

F7781361

F7781362

F7781363

Product Code

KW403-4F semi-micro and micro type

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


KW404-4D

KW404-4B

KW404-2E

KW404-2D

KW404-2B

KW404-1E

KW404-1D

KW404-1B

KW404-M8E

KW404-M8D

KW404-M8B

KW404-M5E

KW404-M5D

KW404-M5B

KW404-M3E

KW404-M3D

KW404-M3B


150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7781412

F7781413

F7781421

F7781422

F7781423

F7781431

F7781432

F7781433

F7781441

F7781442

F7781443

F7781451

F7781452

F7781453

F7781461

F7781462

F7781463

Product Code


2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


KW405-4D

KW405-4B

KW405-2E

KW405-2D

KW405-2B

KW405-1E

KW405-1D

KW405-1B

KW405-M8E

KW405-M8D

KW405-M8B

KW405-M5E

KW405-M5D

KW405-M5B

KW405-M3E

KW405-M3D

KW405-M3B


150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7781512

F7781513

F7781521

F7781522

F7781523

F7781531

F7781532

F7781533

F7781541

F7781542

F7781543

F7781551

F7781552

F7781553

F7781561

F7781562

F7781563

Product Code


Sem

i-mic

ro a

nd

mic

ro c

olu

mn

sA

queous S

EC

(GFC

) colu

mn ; S

ilica-b

ase

d

68

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm

Aqueous SEC(GFC) columns ; Polymer-based

OHpak SB-802 HQ semi-micro and micro type

SB802-4E

SB802-4D

SB802-4B

SB802-2E

SB802-2D

SB802-2B

SB802-1E

SB802-1D

SB802-1B

SB802-M8E

SB802-M8D

SB802-M8B

SB802-M5E

SB802-M5D

SB802-M5B

SB802-M3E

SB802-M3D

SB802-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7770111

F7770112

F7770113

F7770121

F7770122

F7770123

F7770131

F7770132

F7770133

F7770141

F7770142

F7770143

F7770151

F7770152

F7770153

F7770161

F7770162

F7770163

Product Code

* See page 34 for OHpak SB-802 HQ.* See page 79 for the preparative columns.

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm

OHpak SB-802.5 HQ semi-micro and micro type

SB802.5-4E

SB802.5-4D

SB802.5-4B

SB802.5-2E

SB802.5-2D

SB802.5-2B

SB802.5-1E

SB802.5-1D

SB802.5-1B

SB802.5-M8E

SB802.5-M8D

SB802.5-M8B

SB802.5-M5E

SB802.5-M5D

SB802.5-M5B

SB802.5-M3E

SB802.5-M3D

SB802.5-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7770211

F7770212

F7770213

F7770221

F7770222

F7770223

F7770231

F7770232

F7770233

F7770241

F7770242

F7770243

F7770251

F7770252

F7770253

F7770261

F7770262

F7770263

Product Code

* See page 34 for OHpak SB-802.5 HQ.* See page 79 for the preparative columns.

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


SB803-4E

SB803-4D

SB803-4B

SB803-2E

SB803-2D

SB803-2B

SB803-1E

SB803-1D

SB803-1B

SB803-M8E

SB803-M8D

SB803-M8B

SB803-M5E

SB803-M5D

SB803-M5B

SB803-M3E

SB803-M3D

SB803-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7770311

F7770312

F7770313

F7770321

F7770322

F7770323

F7770331

F7770332

F7770333

F7770341

F7770342

F7770343

F7770351

F7770352

F7770353

F7770361

F7770362

F7770363

Product Code


2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


SB804-4E

SB804-4D

SB804-4B

SB804-2E

SB804-2D

SB804-2B

SB804-1E

SB804-1D

SB804-1B

SB804-M8E

SB804-M8D

SB804-M8B

SB804-M5E

SB804-M5D

SB804-M5B

SB804-M3E

SB804-M3D

SB804-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7770411

F7770412

F7770413

F7770421

F7770422

F7770423

F7770431

F7770432

F7770433

F7770441

F7770442

F7770443

F7770451

F7770452

F7770453

F7770461

F7770462

F7770463

Product Code


Aqueous S

EC

(GFC

) colu

mns ; P

olym

er-b

ase

dS

em

i-mic

ro a

nd

mic

ro c

olu

mn

s

69

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


SB805-4E

SB805-4D

SB805-4B

SB805-2E

SB805-2D

SB805-2B

SB805-1E

SB805-1D

SB805-1B

SB805-M8E

SB805-M8D

SB805-M8B

SB805-M5E

SB805-M5D

SB805-M5B

SB805-M3E

SB805-M3D

SB805-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7770531

F7770532

F7770533

F7770521

F7770522

F7770523

F7781431

F7781432

F7781433

F7770541

F7770542

F7770543

F7770551

F7770552

F7770553

F7770561

F7770562

F7770563

Product Code


2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


SB806-4E

SB806-4D

SB806-4B

SB806-2E

SB806-2D

SB806-2B

SB806-1E

SB806-1D

SB806-1B

SB806-M8E

SB806-M8D

SB806-M8B

SB806-M5E

SB806-M5D

SB806-M5B

SB806-M3E

SB806-M3D

SB806-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7770611

F7770612

F7770613

F7770621

F7770622

F7770623

F7770631

F7770632

F7770633

F7770641

F7770642

F7770643

F7770651

F7770652

F7770653

F7770661

F7770662

F7770663

Product Code


Sem

i-mic

ro a

nd

mic

ro c

olu

mn

sA

queous S

EC

(GFC

) colu

mns ; P

olym

er-b

ase

d

70

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm

Multi mode Columns

Asahipak GS-220 HQ semi-micro and micro type

GS220A-4E

GS220A-4D

GS220A-4B

GS220A-2E

GS220A-2D

GS220A-2B

GS220A-1E

GS220A-1D

GS220A-1B

GS220A-M8E

GS220A-M8D

GS220A-M8B

GS220A-M5E

GS220A-M5D

GS220A-M5B

GS220A-M3E

GS220A-M3D

GS220A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7750211

F7750212

F7750213

F7750221

F7750222

F7750223

F7750231

F7750232

F7750233

F7750241

F7750242

F7750243

F7750251

F7750252

F7750253

F7750261

F7750262

F7750263

Product Code

* See page 36 for Asahipak GS-220 HQ.* See page 80 for the preparative columns.

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


GS320A-4E

GS320A-4D

GS320A-4B

GS320A-2E

GS320A-2D

GS320A-2B

GS320A-1E

GS320A-1D

GS320A-1B

GS320A-M8E

GS320A-M8D

GS320A-M8B

GS320A-M5E

GS320A-M5D

GS320A-M5B

GS320A-M3E

GS320A-M3D

GS320A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7750311

F7750312

F7750313

F7750321

F7750322

F7750323

F7750331

F7750332

F7750333

F7750341

F7750342

F7750343

F7750351

F7750352

F7750353

F7750361

F7750362

F7750363

Product Code


2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


GS520A-4E

GS520A-4D

GS520A-4B

GS520A-2E

GS520A-2D

GS520A-2B

GS520A-1E

GS520A-1D

GS520A-1B

GS520A-M8E

GS520A-M8D

GS520A-M8B

GS520A-M5E

GS520A-M5D

GS520A-M5B

GS520A-M3E

GS520A-M3D

GS520A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7750511

F7750512

F7750513

F7750521

F7750522

F7750523

F7750531

F7750532

F7750533

F7750541

F7750542

F7750543

F7750551

F7750552

F7750553

F7750561

F7750562

F7750563

Product Code


2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


GS620A-4E

GS620A-4D

GS620A-4B

GS620A-2E

GS620A-2D

GS620A-2B

GS620A-1E

GS620A-1D

GS620A-1B

GS620A-M8E

GS620A-M8D

GS620A-M8B

GS620A-M5E

GS620A-M5D

GS620A-M5B

GS620A-M3E

GS620A-M3D

GS620A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7750611

F7750612

F7750613

F7750621

F7750622

F7750623

F7750631

F7750632

F7750633

F7750641

F7750642

F7750643

F7750651

F7750652

F7750653

F7750661

F7750662

F7750663

Product Code


Multi m

ode C

olu

mns

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

s

71

Aqueous/Organic SEC Columns

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm

Asahipak GF-310 HQ semi-micro and micro type

GF310A-4E

GF310A-4D

GF310A-4B

GF310A-2E

GF310A-2D

GF310A-2B

GF310A-1E

GF310A-1D

GF310A-1B

GF310A-M8E

GF310A-M8D

GF310A-M8B

GF310A-M5E

GF310A-M5D

GF310A-M5B

GF310A-M3E

GF310A-M3D

GF310A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7760311

F7760312

F7760313

F7760321

F7760322

F7760323

F7760331

F7760332

F7760333

F7760341

F7760342

F7760343

F7760351

F7760352

F7760353

F7760361

F7760362

F7760363

Product Code

* See page 36 for Asahipak GF-310 HQ.* See page 80 for the preparative columns.

2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


GF510A-4E

GF510A-4D

GF510A-4B

GF510A-2E

GF510A-2D

GF510A-2B

GF510A-1E

GF510A-1D

GF510A-1B

GF510A-M8E

GF510A-M8D

GF510A-M8B

GF510A-M5E

GF510A-M5D

GF510A-M5B

GF510A-M3E

GF510A-M3D

GF510A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7760511

F7760512

F7760513

F7760521

F7760522

F7760523

F7760531

F7760532

F7760533

F7760541

F7760542

F7760543

F7760551

F7760552

F7760553

F7760561

F7760562

F7760563

Product Code


2.0mm

4.6mm

1.0mm

0.8mm

0.5mm

0.3mm


GF710A-4E

GF710A-4D

GF710A-4B

GF710A-2E

GF710A-2D

GF710A-2B

GF710A-1E

GF710A-1D

GF710A-1B

GF710A-M8E

GF710A-M8D

GF710A-M8B

GF710A-M5E

GF710A-M5D

GF710A-M5B

GF710A-M3E

GF710A-M3D

GF710A-M3B


250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

250

150

50

F7760711

F7760712

F7760713

F7760721

F7760722

F7760723

F7760731

F7760732

F7760733

F7760741

F7760742

F7760743

F7760751

F7760752

F7760753

F7760761

F7760762

F7760763

Product Code


Sem

i-mic

ro a

nd

mic

ro c

olu

mn

sA

queous

/Org

anic

SE

C C

olu

mns

72

* See page 36 for AFpak AAB-894.

Affinity Chromotography Columns

AFpak AAB-2B

AFpak AAB-2T

AFpak AAB-1B

AFpak AAB-1T

AFpak AAB-M8B

AFpak AAB-M8T

AFpak AAB-M5B

AFpak AAB-M5T

AFpak AAB-M3B

AFpak AAB-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970123

F7970124

F7970133

F7970134

F7970143

F7970144

F7970153

F7970154

F7970163

F7970164

Product Code

AFpak AAB-894 semi-micro and micro type

* See page 52 for AFpak AAF-894.

AFpak AAF-2B

AFpak AAF-2T

AFpak AAF-1B

AFpak AAF-1T

AFpak AAF-M8B

AFpak AAF-M8T

AFpak AAF-M5B

AFpak AAF-M5T

AFpak AAF-M3B

AFpak AAF-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970223

F7970224

F7970233

F7970234

F7970243

F7970244

F7970253

F7970254

F7970263

F7970264

Product Code

AFpak AAF-894 semi-micro and micro type

* See page 52 for AFpak AAM-894.

AFpak AAM-2B

AFpak AAM-2T

AFpak AAM-1B

AFpak AAM-1T

AFpak AAM-M8B

AFpak AAM-M8T

AFpak AAM-M5B

AFpak AAM-M5T

AFpak AAM-M3B

AFpak AAM-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970323

F7970324

F7970333

F7970334

F7970343

F7970344

F7970353

F7970354

F7970363

F7970364

Product Code

AFpak AAM-894 semi-micro and micro type

* See page 52 for AFpak AAP-894.

AFpak AAP-2B

AFpak AAP-2T

AFpak AAP-1B

AFpak AAP-1T

AFpak AAP-M8B

AFpak AAP-M8T

AFpak AAP-M5B

AFpak AAP-M5T

AFpak AAP-M3B

AFpak AAP-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970423

F7970424

F7970433

F7970434

F7970443

F7970444

F7970453

F7970454

F7970463

F7970464

Product Code

AFpak AAP-894 semi-micro and micro type

* See page 52 for AFpak AAV-894.

AFpak AAV-2B

AFpak AAV-2T

AFpak AAV-1B

AFpak AAV-1T

AFpak AAV-M8B

AFpak AAV-M8T

AFpak AAV-M5B

AFpak AAV-M5T

AFpak AAV-M3B

AFpak AAV-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970523

F7970524

F7970533

F7970534

F7970543

F7970544

F7970553

F7970554

F7970563

F7970564

Product Code

AFpak AAV-894 semi-micro and micro type

* See page 52 for AFpak ABA-894.

AFpak ABA-2B

AFpak ABA-2T

AFpak ABA-1B

AFpak ABA-1T

AFpak ABA-M8B

AFpak ABA-M8T

AFpak ABA-M5B

AFpak ABA-M5T

AFpak ABA-M3B

AFpak ABA-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970623

F7970624

F7970633

F7970634

F7970643

F7970644

F7970653

F7970654

F7970663

F7970664

Product Code

AFpak ABA-894 semi-micro and micro type

* See page 52 for AFpak ABT-894.

AFpak ABT-2B

AFpak ABT-2T

AFpak ABT-1B

AFpak ABT-1T

AFpak ABT-M8B

AFpak ABT-M8T

AFpak ABT-M5B

AFpak ABT-M5T

AFpak ABT-M3B

AFpak ABT-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970723

F7970724

F7970733

F7970734

F7970743

F7970744

F7970753

F7970754

F7970763

F7970764

Product Code

AFpak ABT-894 semi-micro and micro type

* See page 52 for AFpak ACA-894.

AFpak ACA-2B

AFpak ACA-2T

AFpak ACA-1B

AFpak ACA-1T

AFpak ACA-M8B

AFpak ACA-M8T

AFpak ACA-M5B

AFpak ACA-M5T

AFpak ACA-M3B

AFpak ACA-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970823

F7970824

F7970833

F7970834

F7970843

F7970844

F7970853

F7970854

F7970863

F7970864

Product Code

AFpak ACA-894 semi-micro and micro type

Affin

ity Chro

moto

gra

phy C

olu

mns

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

s

73

* See page 52 for AFpak ACB-894.

AFpak ACB-2B

AFpak ACB-2T

AFpak ACB-1B

AFpak ACB-1T

AFpak ACB-M8B

AFpak ACB-M8T

AFpak ACB-M5B

AFpak ACB-M5T

AFpak ACB-M3B

AFpak ACB-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7970923

F7970924

F7970933

F7970934

F7970943

F7970944

F7970953

F7970954

F7970963

F7970964

Product Code

AFpak ACB-894 semi-micro and micro type

* See page 52 for AFpak ADS-894.

AFpak ADS-2B

AFpak ADS-2T

AFpak ADS-1B

AFpak ADS-1T

AFpak ADS-M8B

AFpak ADS-M8T

AFpak ADS-M5B

AFpak ADS-M5T

AFpak ADS-M3B

AFpak ADS-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971023

F7971024

F7971033

F7971034

F7971043

F7971044

F7971053

F7971054

F7971063

F7971064

Product Code

AFpak ADS-894 semi-micro and micro type

* See page 52 for AFpak AED-894.

AFpak AED-2B

AFpak AED-2T

AFpak AED-1B

AFpak AED-1T

AFpak AED-M8B

AFpak AED-M8T

AFpak AED-M5B

AFpak AED-M5T

AFpak AED-M3B

AFpak AED-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971123

F7971124

F7971133

F7971134

F7971143

F7971144

F7971153

F7971154

F7971163

F7971164

Product Code

AFpak AED-894 semi-micro and micro type

* See page 52 for AFpak AGA-894.

AFpak AGA-2B

AFpak AGA-2T

AFpak AGA-1B

AFpak AGA-1T

AFpak AGA-M8B

AFpak AGA-M8T

AFpak AGA-M5B

AFpak AGA-M5T

AFpak AGA-M3B

AFpak AGA-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971223

F7971224

F7971233

F7971234

F7971243

F7971244

F7971253

F7971254

F7971263

F7971264

Product Code

AFpak AGA-894 semi-micro and micro type

* See page 52 for AFpak AGE-894.

AFpak AGE-2B

AFpak AGE-2T

AFpak AGE-1B

AFpak AGE-1T

AFpak AGE-M8B

AFpak AGE-M8T

AFpak AGE-M5B

AFpak AGE-M5T

AFpak AGE-M3B

AFpak AGE-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971323

F7971324

F7971333

F7971334

F7971343

F7971344

F7971353

F7971354

F7971363

F7971364

Product Code

AFpak AGE-894 semi-micro and micro type

* See page 52 for AFpak AGT-894.

AFpak AGT-2B

AFpak AGT-2T

AFpak AGT-1B

AFpak AGT-1T

AFpak AGT-M8B

AFpak AGT-M8T

AFpak AGT-M5B

AFpak AGT-M5T

AFpak AGT-M3B

AFpak AGT-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971423

F7971424

F7971433

F7971434

F7971443

F7971444

F7971453

F7971454

F7971463

F7971464

Product Code

AFpak AGT-894 semi-micro and micro type

* See page 52 for AFpak AHR-894.

AFpak AHR-2B

AFpak AHR-2T

AFpak AHR-1B

AFpak AHR-1T

AFpak AHR-M8B

AFpak AHR-M8T

AFpak AHR-M5B

AFpak AHR-M5T

AFpak AHR-M3B

AFpak AHR-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971523

F7971524

F7971533

F7971534

F7971543

F7971544

F7971553

F7971554

F7971563

F7971564

Product Code

AFpak AHR-894 semi-micro and micro type

* See page 52 for AFpak AIA-894.

AFpak AIA-2B

AFpak AIA-2T

AFpak AIA-1B

AFpak AIA-1T

AFpak AIA-M8B

AFpak AIA-M8T

AFpak AIA-M5B

AFpak AIA-M5T

AFpak AIA-M3B

AFpak AIA-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971623

F7971624

F7971633

F7971634

F7971643

F7971644

F7971653

F7971654

F7971663

F7971664

Product Code

AFpak AIA-894 semi-micro and micro type

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

sA

ffinity C

hro

moto

gra

phy C

olu

mns

74

* See page 52 for AFpak ALC-894.

AFpak ALC-2B

AFpak ALC-2T

AFpak ALC-1B

AFpak ALC-1T

AFpak ALC-M8B

AFpak ALC-M8T

AFpak ALC-M5B

AFpak ALC-M5T

AFpak ALC-M3B

AFpak ALC-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971723

F7971724

F7971733

F7971734

F7971743

F7971744

F7971753

F7971754

F7971763

F7971764

Product Code

AFpak ALC-894 semi-micro and micro type

* See page 52 for AFpak ALS-894.

AFpak ALS-2B

AFpak ALS-2T

AFpak ALS-1B

AFpak ALS-1T

AFpak ALS-M8B

AFpak ALS-M8T

AFpak ALS-M5B

AFpak ALS-M5T

AFpak ALS-M3B

AFpak ALS-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971823

F7971824

F7971833

F7971834

F7971843

F7971844

F7971853

F7971854

F7971863

F7971864

Product Code

AFpak ALS-894 semi-micro and micro type

* See page 52 for AFpak ANA-894.

AFpak ANA-2B

AFpak ANA-2T

AFpak ANA-1B

AFpak ANA-1T

AFpak ANA-M8B

AFpak ANA-M8T

AFpak ANA-M5B

AFpak ANA-M5T

AFpak ANA-M3B

AFpak ANA-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7971923

F7971924

F7971933

F7971934

F7971943

F7971944

F7971953

F7971954

F7971963

F7971964

Product Code

AFpak ANA-894 semi-micro and micro type

* See page 52 for AFpak AOV-894.

AFpak AOV-2B

AFpak AOV-2T

AFpak AOV-1B

AFpak AOV-1T

AFpak AOV-M8B

AFpak AOV-M8T

AFpak AOV-M5B

AFpak AOV-M5T

AFpak AOV-M3B

AFpak AOV-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972023

F7972024

F7972033

F7972034

F7972043

F7972044

F7972053

F7972054

F7972063

F7972064

Product Code

AFpak AOV-894 semi-micro and micro type

* See page 52 for AFpak APA-894.

AFpak APA-2B

AFpak APA-2T

AFpak APA-1B

AFpak APA-1T

AFpak APA-M8B

AFpak APA-M8T

AFpak APA-M5B

AFpak APA-M5T

AFpak APA-M3B

AFpak APA-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972123

F7972124

F7972133

F7972134

F7972143

F7972144

F7972153

F7972154

F7972163

F7972164

Product Code

AFpak APA-894 semi-micro and micro type

* See page 52 for AFpak APB-894.

AFpak APB-2B

AFpak APB-2T

AFpak APB-1B

AFpak APB-1T

AFpak APB-M8B

AFpak APB-M8T

AFpak APB-M5B

AFpak APB-M5T

AFpak APB-M3B

AFpak APB-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972223

F7972224

F7972233

F7972234

F7972243

F7972244

F7972253

F7972254

F7972263

F7972264

Product Code

AFpak APB-894 semi-micro and micro type

* See page 52 for AFpak APD-894.

AFpak APD-2B

AFpak APD-2T

AFpak APD-1B

AFpak APD-1T

AFpak APD-M8B

AFpak APD-M8T

AFpak APD-M5B

AFpak APD-M5T

AFpak APD-M3B

AFpak APD-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972323

F7972324

F7972333

F7972334

F7972343

F7972344

F7972353

F7972354

F7972363

F7972364

Product Code

AFpak APD-894 semi-micro and micro type

* See page 52 for AFpak APE-894.

AFpak APE-2B

AFpak APE-2T

AFpak APE-1B

AFpak APE-1T

AFpak APE-M8B

AFpak APE-M8T

AFpak APE-M5B

AFpak APE-M5T

AFpak APE-M3B

AFpak APE-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972423

F7972424

F7972433

F7972434

F7972443

F7972444

F7972453

F7972454

F7972463

F7972464

Product Code

AFpak APE-894 semi-micro and micro type

Affin

ity Chro

moto

gra

phy C

olu

mns

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

s

75

* See page 52 for AFpak APG-894.

AFpak APG-2B

AFpak APG-2T

AFpak APG-1B

AFpak APG-1T

AFpak APG-M8B

AFpak APG-M8T

AFpak APG-M5B

AFpak APG-M5T

AFpak APG-M3B

AFpak APG-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972523

F7972524

F7972533

F7972534

F7972543

F7972544

F7972553

F7972554

F7972563

F7972564

Product Code

AFpak APG-894 semi-micro and micro type

* See page 52 for AFpak APH-894.

AFpak APH-2B

AFpak APH-2T

AFpak APH-1B

AFpak APH-1T

AFpak APH-M8B

AFpak APH-M8T

AFpak APH-M5B

AFpak APH-M5T

AFpak APH-M3B

AFpak APH-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972623

F7972624

F7972633

F7972634

F7972643

F7972644

F7972653

F7972654

F7972663

F7972664

Product Code

AFpak APH-894 semi-micro and micro type

* See page 52 for AFpak APR-894.

AFpak APR-2B

AFpak APR-2T

AFpak APR-1B

AFpak APR-1T

AFpak APR-M8B

AFpak APR-M8T

AFpak APR-M5B

AFpak APR-M5T

AFpak APR-M3B

AFpak APR-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972723

F7972724

F7972733

F7972734

F7972743

F7972744

F7972753

F7972754

F7972763

F7972764

Product Code

AFpak APR-894 semi-micro and micro type

* See page 52 for AFpak ARC-894.

AFpak ARC-2B

AFpak ARC-2T

AFpak ARC-1B

AFpak ARC-1T

AFpak ARC-M8B

AFpak ARC-M8T

AFpak ARC-M5B

AFpak ARC-M5T

AFpak ARC-M3B

AFpak ARC-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972823

F7972824

F7972833

F7972834

F7972843

F7972844

F7972853

F7972854

F7972863

F7972864

Product Code

AFpak ARC-894 semi-micro and micro type

* See page 52 for AFpak AST-894.

AFpak AST-2B

AFpak AST-2T

AFpak AST-1B

AFpak AST-1T

AFpak AST-M8B

AFpak AST-M8T

AFpak AST-M5B

AFpak AST-M5T

AFpak AST-M3B

AFpak AST-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7972923

F7972924

F7972933

F7972934

F7972943

F7972944

F7972953

F7972954

F7972963

F7972964

Product Code

AFpak AST-894 semi-micro and micro type

* See page 52 for AFpak AWG-894.

AFpak AWG-2B

AFpak AWG-2T

AFpak AWG-1B

AFpak AWG-1T

AFpak AWG-M8B

AFpak AWG-M8T

AFpak AWG-M5B

AFpak AWG-M5T

AFpak AWG-M3B

AFpak AWG-M3T

Length(mm)

2.0mm

1.0mm

0.8mm

0.5mm

0.3mm

Product NameI.D.

50

35

50

35

50

35

50

35

50

35

F7973023

F7973024

F7973033

F7973034

F7973043

F7973044

F7973053

F7973054

F7973063

F7973064

Product Code

AFpak AWG-894 semi-micro and micro type

Sem

i-mic

ro a

nd

mic

ro c

olu

mn

sA

ffinity C

hro

moto

gra

phy C

olu

mns

76

Polymer-based Packed Columns for Reversed Phase Chromatography

Contact Shodex or our distributors near you for larger preparative columns.

F6820001

F6820035

F6820019

F6710004

F6714029

Asahipak ODP-50 10E

Asahipak ODP-90 20F

Asahipak ODP-130 28F

Asahipak ODP-130G 7B

Asahipak ODP-130G 20C

5

9

13

13

13

10.0 x 250

20.0 x 300

28.0 x 300

7.5 x 50

20.0 x 100

Product Code Product Name Plate Number (TP/column)



Particle Size (μm)

≧ 10,000

≧ 9,000

≧ 9,000

(guard column)

(guard column)

* See page 6 for Asahipak ODP-50.* See page 64 for the semi-micro columns and micro columns.

Preparative columns (I.D. 10mm~50mm)

Asahipak ODP-50 Preparative type

F6513013

F6700190

F6513015

F6700191

RSpak DE-2013

RSpak DE-LG

RSpak DE-5013

RSpak DE-LLG

12

12

12

12

20.0 x 300

8.0 x 50

50.0 x 300

20.0 x 100



Particle Size (μm)

≧ 10,000

(guard column)

-—

(guard column)

* See page 8 for RSpak DE-613.* See page 64 for the semi-micro columns and micro columns.

RSpak DE-613 Preparative type

F6514014

F6700404

F6514022

F6700162

RSpak DＭ-2014

RSpak DＭ-LG

RSpak DM-5014

RSpak DM-LLG

12

12

12

12

20.0 x 300

8.0 x 50

50.0 x 300

20.0 x 100



Particle Size (μm)

≧ 5,000

(guard column)

-—

(guard column)

* See page 8 for RSpak DM-613.

RSpak DM-614 Preparative type

F6820003

F6714004

Asahipak C8P-50 10E

Asahipak C8P-50G 7B

5

5

10.0 x 250

7.5 x 50


Particle Size (μm)

≧ 8,000

(guard column)

* See page 6 for Asahipak C8P-50.

Asahipak C8P-50 Preparative type


F6820005

F6714005

Asahipak C4P-50 10E

Asahipak C4P-50G 7B

5

5

10.0 x 250

7.5 x 50


Particle Size (μm)

≧ 7,000

(guard column)

* See page 6 for Asahipak C4P-50.

Asahipak C4P-50 Preparative type

Polym

er-b

ase

d P

acked C

olu

mns fo

r Reve

rsed

Ph

ase

Ch

rom

ato

gra

ph

yP

repara

tivecolu

mns

77

Silica-based Packed Columns for Reversed Phase Chromatography

Polymer-based Packed Columns for Normal Phase Chromatography

Silica-based Packed Columns for Normal Phase Chromatography

F6830001

F6830031

F6830007

F6710017

Asahipak NH2P-50 10E

Asahipak NH2P-90 20F

Asahipak NH2P-130 28F

Asahipak NH2P-130G 7B

5

9

13

13

10.0 x 250

20.0 x 300

28.0 x 300

7.5 x 50



Particle Size (μm)

≧ 10,000

≧ 10,000

≧ 1,000

(guard column)

* See page 10 for Asahipak NH2P-50.* See page 64 for the semi-micro columns and micro columns.

Asahipak NH2P-50 Preparative type

F6514013

F6700402

F6514021

F6700172

RSpak DC-2013

RSpak DC-LG

RSpak DC-5013

RSpak DC-LLG

10

10

10

10

20.0 x 300

8.0 x 50

50.0 x 300

20.0 x 100



Particle Size (μm)

≧ 6,000

(guard column)

-—

(guard column)

* See page 10 for RSpak DC-613.

RSpak DC-613 Preparative type

F7560040

F7560041

Silica C18M 10E

Silica C18M 20E

5

5

10.0 x 250

20.0 x 250



Particle Size (μm)

≧ 16,000

≧ 16,000

* See page 12 for Silica C18M.

Silica C18M Preparative type

F7560062

F7560063

Silica 5C8 10E

Silica 5C8 20E

5

5

10.0 x 250

20.0 x 250



Particle Size (μm)

≧ 15,000

≧ 15,000

* See page 12 for Silica 5C8.

Silica 5C8 Preparative type

F7560054

F7560055

Silica 5C4 10E

Silica 5C4 20E

5

5

10.0 x 250

20.0 x 250



Particle Size (μm)

≧ 15,000

≧ 15,000

* See page 12 for Silica 5C4.

Silica 5C4 Preparative type

F7560060

F7560061

Silica 5NH 10E

Silica 5NH 20E

5

5

10.0 x 250

20.0 x 250



Particle Size (μm)

≧ 8,000

≧ 8,000

* See page 12 for Silica 5NH.

Silica 5NH Preparative type

F7560050

F7560051

Silica 5SIL 10E

Silica 5SIL 20E

5

5

10.0 x 250

20.0 x 250



Particle Size (μm)

≧ 15,000

≧ 15,000

* See page 12 for Silica 5SIL

Silica 5SIL Preparative type

Pre

para

tivecolu

mns

Polym

er-b

ase

d P

acked C

olu

mns fo

r Norm

al P

hase

Ch

rom

ato

gra

ph

yS

ilica-b

ase

d P

acked C

olu

mns fo

r Reve

rsed P

hase

Ch

rom

ato

gra

ph

y Silic

a-b

ase

d P

acked

Colu

mns fo

r Norm

al P

hase

Chro

mato

gra

phy

78

Columns for Anion Exchange Chromatography

Columns for Cation Exchange Chromatography

F6548002

F6548022

F6548052

F6709604

IEC SP-2025

IEC SP-2825

IEC SP-5025

IEC SP-LG

20

37

37

20

20.0 x 150

28.0 x 250

50.0 x 300

8.0 x 50

Product Code Product Name Column Size (mm)I.D. x L

Particle Size (μm)

* See page 14 for IEC SP-825.* See page 65 for the semi-micro columns and micro columns.

IEC SP-825 Preparative type

F6548003

F6548023

F6548053

F6709605

IEC CM-2025

IEC CM-2825

IEC CM-5025

IEC CM-LG

20

37

37

20

20.0 x 150

28.0 x 250

50.0 x 300

8.0 x 50


Particle Size (μm)

* See page 14 for IEC CM-825.* See page 65 for the semi-micro columns and micro columns.

IEC CM-825 Preparative type

F6548000

F6548020

F6548050

F6709602

IEC QA-2025

IEC QA-2825

IEC QA-5025

IEC QA-LG

20

37

37

20

20.0 x 150

28.0 x 300

50.0 x 300

8.0 x 50


Particle Size (μm)

* See page 16 for IEC QA-825.* See page 66 for the semi-micro columns and micro columns.

IEC QA-825 Preparative type

F6548001

F6548021

F6598051

F6709603

IEC DEAE-2025

IEC DEAE-2825

IEC DEAE-5025

IEC DEAE-LＧ

20

37

37

20

20.0 x 150

28.0 x 250

50.0 x 300

8.0 x 50


Particle Size (μm)

* See page 16 for IEC DEAE-825.* See page 66 for the semi-micro columns and micro columns.

IEC DEAE-825 Preparative type

F6840004

F6710021

Asahipak ES-502N 20C

Asahipak GS-20G 7B

13

20

20.0 x 100

7.5 x 50


Particle Size (μm)

* See page 16 for Asahipak ES-502N.* See page 66 for the semi-micro columns and micro columns.

Asahipak ES-502N Preparative type

F6840003

F6710021

Asahipak ES-502C 20C

Asahipak GS-20G 7B

13

20

20.0 x 100

7.5 x 50


Particle Size (μm)

* See page 14 for Asahipak ES-502C.* See page 65 for the semi-micro columns and micro columns.

Asahipak ES-502C Preparative type

Colu

mns fo

r Catio

n E

xchange C

hro

mato

gra

ph

y Colu

mn

s for A

nio

n E

xch

an

ge C

hro

mato

gra

phy

Pre

para

tivecolu

mns

79

Columns for Saccharides Analysis

KS-801

KS-802

KS-803

KS-804

KS-805

KS-806

(guard column)

* See page 20 for SUGAR KS-800.

Aqueous SEC(GFC) columns ; Polymer-based

Aqueous SEC(GFC) columns ; Silica-based

Columns for Organic Acids Analysis

F6502007

F6502008

F6502009

F6502010

F6502011

F6502012

F6700002

Product Code

SUGAR KS-2001

SUGAR KS-2002

SUGAR KS-2003

SUGAR KS-2004

SUGAR KS-2005

SUGAR KS-2006

SUGAR KS-LG

Product Name Standard Column

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

8.0 x 50


13

13

13

18

18

18

13

Particle Size (μm)

SUGAR KS-800 series Preparative typePlate Number (TP/column)

≧ 7,000

≧ 7,000

≧ 8,000

≧ 6,000

≧ 6,000

≧ 6,000

(guard column)

SB-802 HQ

SB-802.5 HQ

SB-803 HQ

SB-804 HQ

SB-805 HQ

SB-806 HQ

SB-806M HQ

(guard column)

* See page 34 for OHpak SB-800 HQ.* See page 67 for the semi-micro columns and micro columns.

F6516011

F6516012

F6516013

F6516014

F6516015

F6516016

F6516017

F6709555

Product Code

OHpak SB-2002

OHpak SB-2002.5

OHpak SB-2003

OHpak SB-2004

OHpak SB-2005

OHpak SB-2006

OHpak SB-2006M

OHpak SB-LG


20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

8.0 x 50


15

10

10

18

20

20

13

18

Particle Size (μm)

OHpak SB-800 HQ series Preparative typePlate Number (TP/column)

≧ 9,000

≧ 12,000

≧ 12,000

≧ 12,000

≧ 12,000

≧ 12,000

≧ 12,000

(guard column)

KW-802.5

KW-803

KW-804

(guard column)

* See page 32 for PROTEIN KW-800.* See page 67 for the semi-micro columns and micro columns.

F6505020

F6505021

F6505022

F6709556

Product Code

PROTEIN KW-2002.5

PROTEIN KW-2003

PROTEIN KW-2004

PROTEIN KW-G


20.0 x 300

20.0 x 300

20.0 x 300

8.0 x 50


5

5

7

7

Particle Size (μm)

PROTEIN KW-800 series Preparative typePlate Number (TP/column)

≧ 17,000

≧ 17,000

≧ 14,000

(guard column)

* See page 24 for RSpak KC-811.

F6505012

F6700010

Product Code

RSpak KC-2011

RSpak KC-LG

Product Name

20.0 x 300

8.0 x 50


13

13

Particle Size (μm)

RSpak KC-811 Preparative typePlate Number (TP/column)

≧ 8,000

(guard column)

Pre

para

tivecolu

mns

Colu

mns fo

r Saccharid

es A

nalysis C

olu

mn

s for O

rgan

ic A

cid

s An

alysis A

qu

eou

s SE

C(G

FC

) colu

mns ; S

ilica-b

ase

dA

queous S

EC

(GFC

) colu

mns ; P

olym

er-b

ase

d

80

Multi mode columns

Aqueous/Organic SEC columns

Organic SEC (GPC) columns

GS-220 HQ

GS-320 HQ

GS-520 HQ

GS-620 HQ

GS-220 HQ

GS-320 HQ

GS-520 HQ

GS-620 HQ

(guard column)

* See page 36 for Asahipak GS-HQ.* See page 70 for the semi-micro columns and micro columns.

F6810017

F6810018

F6810019

F6810020

F6810034

F6810035

F6810036

F6810037

F6710021

Product Code

Asahipak GS-220 20F

Asahipak GS-320 20F

Asahipak GS-520 20F

Asahipak GS-620 20F

Asahipak GS-220 20G

Asahipak GS-320 20G

Asahipak GS-520 20G

Asahipak GS-620 20G

Asahipak GS-20G 7B


20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

7.5 x 50


13

13

13

13

13

13

13

13

20

Particle Size (μm)

Asahipak GS-HQ series Preparative typePlate Number (TP/column)

≧ 8,000

≧ 8,000

≧ 8,000

≧ 8,000

≧ 14,000

≧ 14,000

≧ 14,000

≧ 14,000

(guard column)

GF-310 HQ

GF-510 HQ

GF-710 HQ

GF-7M HQ

GF-310 HQ

GF-510 HQ

GF-710 HQ

GF-7M HQ

(guard column)

* See page 38 for Asahipak GF-HQ.* See page 71 for the semi-micro columns and micro columns.

F6810030

F6810031

F6810032

F6810033

F6810038

F6810039

F6810040

F6810041

F6710020

Product Code

Asahipak GS-310 20F

Asahipak GS-510 20F

Asahipak GS-710 20F

Asahipak GSM-700 20F

Asahipak GS-310 20G

Asahipak GS-510 20G

Asahipak GS-710 20G

Asahipak GSM-700 20G

Asahipak GS-10G 7B


20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

7.5 x 50


13

13

13

13

13

13

13

13

20

Particle Size (μm)

Asahipak GF-HQ series Preparative typePlate Number (TP/column)

≧ 8,000

≧ 8,000

≧ 8,000

≧ 8,000

≧ 14,000

≧ 14,000

≧ 14,000

≧ 14,000

(guard column)

K-801

K-802

K-802.5

K-803

K-804

K-805

K-806

K-806M

(guard column)

* See page 40 for GPC K-800.

F6102301

F6102312

F6102315

F6102303

F6102304

F6102305

F6102306

F6102309

F6700407

Product Code

GPC K-2001

GPC K-2002

GPC K-2002.5

GPC K-2003

GPC K-2004

GPC K-2005

GPC K-2006

GPC K-2006M

GPC K-LG


20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

8.0 x 50


6

6

6

6

7

10

10

10

15

Particle Size (μm)

GPC K-800 sereis Preparative typePlate Number (TP/column)

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 14,000

≧ 10,000

≧ 10,000

≧ 10,000

(guard column)

Multi m

ode c

olu

mns A

queous/O

rganic

SE

C c

olu

mn

s Org

an

ic S

EC

(GP

C) c

olu

mn

sP

repara

tivecolu

mns

81


Custom-made Preparative Columns


KF-801

KF-802

KF-802.5

KF-803

KF-804

KF-805

KF-806

KF-806M

(guard column)

* See page 40 for GPC KF-800.

F6102401

F6102402

F6102425

F6102403

F6102404

F6102405

F6102406

F6102409

F6700406

Product Code

GPC KF-2001

GPC KF-2002

GPC KF-2002.5

GPC KF-2003

GPC KF-2004

GPC KF-2005

GPC KF-2006

GPC KF-2006M

GPC KF-LG


20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

20.0 x 300

8.0 x 50


6

6

6

6

7

10

10

10

15

Particle Size (μm)

GPC KF-800 series Preparative typePlate Number (TP/column)

≧ 18,000

≧ 18,000

≧ 18,000

≧ 18,000

≧ 14,000

≧ 10,000

≧ 10,000

≧ 10,000

(guard column)

K-801

K-802

K-802.5

K-803

K-804

K-805

K-806

K-806M

(guard column)

* See page 40 for GPC K-800.

F6102001

F6102002

F6102025

F6102003

F6102004

F6102005

F6102006

F6102009

F6700310

Product Code

GPC H-2001

GPC H-2002

GPC H-2002.5

GPC H-2003

GPC H-2004

GPC H-2005

GPC H-2006

GPC H-2006M

GPC H-LG


20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

20.0 x 500

8.0 x 50


15

15

15

15

15

15

15

15

15

Particle Size (μm)

GPC K-800 series Preparative typePlate Number (TP/column)

≧ 13,000

≧ 13,000

≧ 13,000

≧ 13,000

≧ 13,000

≧ 13,000

≧ 13,000

≧ 12,000

(guard column)

KF-801

KF-802

KF-802.5

KF-803

KF-804

(guard column)

* See page 40 for GPC KF-800.

F6108010

F6108020

F6108025

F6108030

F6108040

F6700408

Product Code

GPC KF-5001

GPC KF-5002

GPC KF-5002.5

GPC KF-5003

GPC KF-5004

GPC KF-LLG


50.0 x 300

50.0 x 300

50.0 x 300

50.0 x 300

50.0 x 300

20.0 x 100


15

15

15

15

15

15

Particle Size (μm)

GPC KF-800 series Preparative type

Pre

para

tivecolu

mns

Org

anic

SE

C (G

PC

) colu

mns C

usto

m-m

ade P

rep

ara

tive C

olu

mn

s

CH2C4H9 H

n

CH

CH2OO

O

HH H

HH

HOH

OH

OH

CH2OHO

HH

H OH

OH

CH2OHO

HH

H OHn

OHO

H H H

82

F8604075 STANDARD M-75

M-949M-451M-139M-53M-21M-7.1M-1.9

949,000451,000139,000

52,60020,800

7,1001,890

1.05 1.021.05 1.02 1.03 1.08 1.10

Std. No. Mp Mw/Mn

Note : Molecular weights (Mw/Mn and Mp) may vary depending on production lots even in the same type of Shodex STANDARD.

SL-105

F8601105

F8602105

F8603075

0.5g x 10 kinds

0.5g x 10 kinds

0.5g x 7 kinds

0.5g x 7 kinds

0.2g x 8 kinds

STANDARD SL-105

STANDARD SM-105

STANDARD SH-75

Structure formula of S series

Structure formula of P series

S-20S-11S-6.9S-5.0S-4.4S-2.9S-1.9S-1.2S-1.0S-0.5

19,80010,7006,9305,0304,4302,9001,9301,2001,050

580

1.021.021.031.031.031.031.051.071.071.12

Std. No. Mp Mw/MnS-3730S-2480S-1230S-579S-197S-55.1S-31.4S-12.8S-3.95S-1.20

3,730,0002,480,0001,230,000

579,000197,000

55,10031,40012,8003,9501,200

1.041.051.051.021.021.031.021.021.031.07

Std. No. Mp Mw/MnS-7450S-3790S-3250S-2220S-1820S-1060S-591

7,450,0003,790,0003,250,0002,220,0001,820,0001,060,000

591,000

1.071.051.041.031.041.031.03

Std. No. Mp Mw/Mn

Note : Molecular weights (Mw/Mn and Mp) may vary depending on production lots even in the same type of Shodex STANDARD.

5,000～800,000F8400000 STANDARD P-82

P-800P-400P-200P-100P-50P-20P-10P-5

788,000404,000212,000112,000

47,30022,80011,800

5,900

1.23 1.13 1.13 1.12 1.06 1.07 1.10 1.09

Std. No. Mw708,000375,000200,000107,000

47,10021,10011,100

5,900

MpMw/Mn

Note :Molecular weights (Mw, Mw/Mn and Mp) may vary depending on production lots even in the same type of Shodex STANDARD.

Calib

ratio

n S

tandard

s for S

EC

Calibration standards

for SEC

Calibration Standards for SECIt is necessary to plot a calibration curve using the known molecular weight of a standard substance to determine the molecular weight distribution of polymers in size exclusion chromatography (SEC).Shodex STANDARD is recommended as the standard substance.Shodex STANDARD are stable standard polymer with a very narrow molecular weight range and minimal side chains. Three kinds of compounds are available: polystyrene (PS), polymethylmethacrylate (PMMA), and pullulan.

Features

• For organic SEC(GPC)• High linearity polystyrene with anionic polymerization• Soluble in tetrahydrofuran (THF), chloroform, toluene, and o-dichlorobenzene

SL-105, SM105, SH-75

[Polystyrene (PS)]

Features

• For organic SEC(GPC)• Narrow molecular weight range• Soluble in hexafluoroisopropanol (HFIP) and dimethyformamide (DMF).

M-75

[Polymethylmethacrylate (PMMA)]

Product Name Contents MW RangeProduct Code



500～22,000

1,200～3,800,000

590,000～7,500,000

SM-105 SH-105

1,800～950,000

Features

• For aqueous SEC(GPC)• Has no side chains• High solubility in water eliminates the possibility of recrystallization.

P-82

[Pullulan]

An altered peak shape and elution time or an elevated column pressure may be resolved by washing the column. This section describes typical warning signs of a deteriorating column and the method for washing column. For detailed washing procedure, refer to the corresponding operation manual.

General precautions for handling a columnFor the proper use of a column, follow the instructions described below.

83

Typical findings observed in a deteriorated (contaminated) column

Column attachmentFlush your HPLC system (flow lines, sample loop, degasser etc.) with new eluent before connecting the column to it. (When the new eluent is not miscible with the solution that is present in the system or causes salt to precipitate, flush with a solution that is compatible with both before flushing the eluent.)Connect the column to the system while flushing with eluent at a low flow rate. Make sure that the eluent flows in the direction indicated by the arrow of the flow mark.When warming the column, use a low flow rate until the temperature reaches a desired level and then slowly increase the flow rate to the measurement flow rate.

Column detachment and storageWhen the column has been warmed, do not turn off the pump suddenly. Wait until the column has cooled to room temperature while keeping the flow rate low.Turn off the pump after the column has cooled to room temperature.

Column storageFor long-term storage, substitute the solution remaining in the column with the shipping solvent and remove the column from the system, then cap both ends.Store the column in a temperature-stabilized place.For the long-term storage of SEC columns, it is recommended that you store the column in a fluid. * See the operation manual for the method of storing the column in a fluid.

OtherAvoid impacts to the column. Be careful not to drop the column from a higher position.Do not bend the column.Avoid opening the end fitting, column performance may deteriorate.

Reversed phase columns

To wash the column efficiently, connect the column inversely and reduce the flow rate to one third of the usual value.

Ion exchange columns(includeing IC columns)

Sugar analysis columns

Hydrophobic interaction columns

Aqueous SEC (GFC) columns

* In general, washing with solvent by 5 to 10 times of column volume is recommended. * Avoid pressure elevation during washing. * A seriously contaminated column will not achieve complete recovery.

For your informationOne cause of elevated column pressure could be clogging of solid substances in the sintered filter mounted at the opening of the column.In this case, connect the column inversely and reduce the flow rate to one third of the usual one or lower. This may decrease the pressure.

Flush with a solvent containing a high content of methanol, acetonitrile, THF, or other organic solvent.(Check salt concentration before washing. Flushing too high concentration of organic solvent may cause the salt to precipitate.)

For ionic adsorption ··················· Flush with a solution containing a high content of salt or having a pH different from that of the solution used in the analysis.For hydrophobic adsorption ······· Flush with a solvent containing a small percentage of organic solvent.For proteins ······························· Inject 1 to 2 mL of 0.1 M NaOH or 30% acetic acid several times.

[SUGAR series ; ligand exchange columns]For counter ion detachment·········Flush with a salt solution containing the same counter ion or inject such a solution several times.

[NH2P-50 ; polymer-based amino columns]For amino group modifications·····Flush 0.1 M perchloric acid, 0.1 M NaOH, and eluent while allowing water to pass through the column ahead of each solution.

For proteins : Inject 1 to 2 mL of 0.1 M NaOH or 30% acetic acid several times.

For ionic adsorption ···················· Flush with a solution containing a high content of salt or a pH different from that of the solution used in the analysis.For hydrophobic adsorption ······ Flush with a solvent containing a small percentage of organic solvent.(Check salt concentration before washing. Flushing too high concentration of organic solvent may cause the salt to precipitate.)

1. Elevated column pressure

2. Abnormal peak shapes (broad, leading, or tailing) and split peaks

3. Altered retention times

4. Unstable baseline values

The solvent should be capable of dissolving the adsorbed substances (contaminants).

The solvent should be able to pass through the relevant column at a high flow rate.

* Use only solvents specified as usable solvents in the operation manual.

* See operation manual before using column

Column Washing Info

rmatio

nC

olu

mn W

ash

ing G

enera

l pre

cautio

ns fo

r han

dlin

g a

colu

mn

Points to consider in the selection of a washing solvent

Standard washing methods

* Use only solvents specified as usable solvents in the operation manual.

1.0

1.1

1.2

1.3

1.4

1.5

Sucrose (20ng)

Sucrose (20ng)

Fructose (20ng)

Fructose (20ng)

mV

mV

-0.1

0.0

0.1

0.2

0.3

0.4

NEW

84

Product Code

Model

Flow cell type

Measurement method

Refractive Index range

Measurement range

Drift *

Linearity range

Noise **

Response

Auto zero

Auto zero range

Off-set range

Off-set resolution

Cell volume

Flow rate (Usual)

(Max.)

Maximum back pressure

Internal volume

Recorder output

External input

External Output

Temperature control

Communication port

Operator support function

Wetted materials

Power source, Power consumption

Dimension, Weight

Accessories

3 chamber-type

Deflection type

1.00~1.75

0.125~256μRIU

0.1μRIU/h

≧ 300μ RIU

≦ 1nRIU

0.1, 0.25, 0.5, 1, 1.5, 2, 3, 6sec

Full auto zero

All range

5μRIU

25nRIU

DC 0~1V(4mV/μRIU, 16mV/μRIU)

8μL

0.2~3.0mL/min

10mL/min (solvent ; pure water)

50kPa

IN→Cell ; 80μLCell→OUT ; 600μLAll (Cell→OUT) ; 690μL

0~10mV/FS

—

Analysis

2 chamber-type

F4010105 F4010106

Shodex RI-201 higher performance

reasonable price

Refractive Index Detector

A new refractive index (RI) detector series is now available.RI-201 is a highly sensitive RI detector incorporating a three-chamber flow cell.

NEW

Shodex RI-201HRefractive Index Detector

• A novel optical system (three-chamber flow cell) provides twice or better sensitivity than that of our previous detectors.

• The two-step temperature control method significantly reduces drift due to the fluctuation of room temperature.

• The detection limit for saccharides is approximately 2 ng.

In our previous optical system, a measurement

light passing through the flow cell is refracted

only once. The new three-chamber flow cell

allows the light to be refracted twice, thereby

increases sensitivity at least twice. This doubles

the degree of polarization light and thus halves

the noise and the influence of drift.

3 chamber-type flow cell

3 chamber-type flow cell

Application

Current 2 chamber-type flow cell

Current 2 chamber-type flow cell

sample cell

reference cell

θ θ2θ

RI-201 RI-100 series, RI-201H

Refra

ctive

Index D

ete

cto

r : Shodex R

I-201 S

hod

ex R

I-20

1H

Instru

me

nts

Features

• Adopts the same optical system as that of RI-101. (2 chamber-type)• Reasonable price

Features

Principle of new optical system

0.25~512μRIU

0.2μRIU/h

≧ 600μ RIU

≦ 2.5nRIU

10μRIU

50nRIU


IN→Cell ; 60μLCell→OUT ; 600μLAll (Cell→OUT) ; 670μL

① READY (temperature control)② LEAK ③ ERROR (ROM, RAM, PARAMETER, HOME-POSITION, OVER-HEAT, OPT.-BALANCE, INTENSITY)

OFF, 30~50˚C (1˚C step), 77˚C Temp. fuse (Double Temperature control)

USB

None

Stainless steel 316, Teflon, Quartz Glass

AC100~240V±10%, 50/60Hz, 150VA max

W260 x D400 x H150 (mm), ca. 12kg

Power cable, signal code, connector tube, fuse, operation manual

*Pure water 1mL/min, PURGE OFF**Pure water, response : 1.5sec

RI-201 RI-201H

Integrator output(Sensitivity)

85

The Shodex RI-100 series includes versatile and highly sensitive RI detectors equipped with a color liquid crystal display, an automatic start-up function, and a validation wizard, and can be used with various HPLC systems.

• Equipped with a color liquid crystal display, the detector monitors the status of the chromatograph in real time.

• The automatic start-up function automates complicated operations such as the substituting eluent in the reference cell and checking the baseline stabilization.

• The validation wizard achieves component validation with ease.• Optimization of the temperature control method shortens the time

for stabilization after the start-up and improves baseline stability.• The leak sensor automatically stops the pump in case of solvent

leakage.• External input and output terminals and RS232C communication

ports permit advanced automation of the system.

Features

*Pure water 1mL/min, PURGE OFF**Pure water, response : 1.5sec

Product Code

Model

Flow cell type

Measuring method

Refractive Index range

Measurement range

Drift *

Linearity range

Noise **

Response

Auto zero

Auto zero range

Off-set range

Off-set resolution

Cell volume

Flow rate (Usual)

(Max.)

Maximum back pressure

Internal volume

Recorder output

External input

External output

Temperature control

Communication port

Operational support functions

Wetted materials

Power source, Power consumption

Dimensions, Weight

Accessories

① READY (Automatic start-up)② LEAK ③ ERROR (OVER HEAT/LOW LIGHT INTENSITY/NULL GLASS HOME POSITION/LOST PARAMETERS/OPTICAL BALANCE) (Contact capacity : DC24V 0.1A max.)

F4010101

RI-101

Analysis

2 chamber-type

Deflection type

1.00~1.75

0.25～512μRIU

0.2μRIU/h

≧ 600μRIU

≦ 2.5nRIU

0.1, 0.25, 0.5, 1, 1.5, 2, 3, 6sec

Full auto zero

All range

0~500mV (Same as integrator output)

10mV (Same as integrator output)

F4010104

RI-104

Semi-micro

F4010102

RI-102

Preparative

0.25～512μRIU

0.2μRIU/h

≧ 600μRIU

≦ 5nRIU

2.5～5120μRIU

2μRIU/h

≧ 6000μRIU

≦ 25nRIU


8μL

0.2~3.0mL/min


50kPa

0~10mV/FS

Purge On/Off, Auto Zero, Marker


2.5μL

0.2~1.0mL/min

1.0mL/min (solvent ; pure water)

DC 0~1V（0.2mV/μRIU, 0.8mV/μRIU）

8μL

1.0～50mL/min


IN→Cell : ca. 60μLCell→OUT : ca. 600μLAll (Cell→OUT) : ca. 670μL



Shodex RI-100 seriesRefractive Index Detector Instru

me

nts

Refra

ctive

Index D

ete

cto

r : Shodex R

I-100 se

ries

OFF, 30~50˚C (1˚C step), 77˚C Temp. fuse

RS232C

Stainless steel 316, Teflon, Quarts glass

AC100~240±10%, 50/60Hz, 150VA max

W260 x D400 x H200 (mm), ca.13kg

Power cable, signal code, connector tube, fuse, operation manual

① Automatic start-up (Start Up Sequence) ② Span/Validation Guide ③ Real Time Baseline Monitor

Integrator output(Sensitivity)

86

Instru

me

nn

ts

The liquid to be degassed runs through a membrane made of special synthetic resin membrane in a vacuum chamber. Dissolved gas in the liquid passes through the membrane and eliminated from the liquid because of its small molecular size, higher mobility than that of the liquid, and higher affinity with the special synthetic resin membrane.

Principle

• Pressure sensor and leak monitor assure high safety.• Dead volume is minimized to 7mL/channel.• Any pump can be used because differential pressure is low.• Continuous degassing mode helps eluent preparation for high-sensitivity analysis.• EMC and LVD compliance, with CE marking

Features

Product Code

Model

Solvent Channels

Degassing capacity

Internal volume

Max. Flow Rate

External output

Dimensions

Power source

Functions, Displays

Weight

DEGASSER® ERC-3000α seriesERC-3115α, 3215α, 3315α, 3415α

Eluent Degassing Device

*[DEGASSER®] is the registered trademark of E.R.C. Co., Ltd.

Feed pump to outlet

Eluent Vacuum pump

Membrane degasser

Vacuum chamber Pressure sensor

Control board

Three-way solenoid valve

Y4617007

ERC-3115α

1 channel

7mL/Channel

20mL/min for each channel (Eluent : 25˚C Pure water)

W71 x D310 x H136 (mm)

AC100V~AC240V, 50/60Hz

When ion-exchanged water saturated with air at 25˚C is put through at a flow rate of 3 mL/min, no bubble is observed at the outlet of the apparatus.(Measured dissolved oxygen level at the outlet: 2 ppm at flow rate of 3 mL/min)

• Power On/Off display : "POWER" LED lights, when the power is supplied. • Status monitoring function : "READY" LED lights, when the internal pressure in the vacuum chamber is below a predetermined limit. • Pressure monitoring function : "PRES" LED lights, when the internal pressure in the vacuum chamber does not reach a predetermined level within a predetermined time. • Leak monitor : "LEAK" LED lights, when the liquid leaks in the apparatus. • Self cleaning : The vacuum line is cleaned by air suction. • Vacuum pump operation switching function: NORM. : Controlled operation in normal run CONT. : Continuous operation in case of high degree of degassing

An open connector signal is delivered to the external output signal terminal, when "PRES" and "LEAK" LEDs light.

Y4617000

ERC-3215α

2 channels

Y4617002

ERC-3315α

3 channels

Y4617004

ERC-3415α

4 channels

ca. 5.0kg ca. 5.1kg ca. 5.4kg ca. 5.5kg

* For use of special solvents, such as fluorinated organic solvents, please contact Shodex or our distributors near you.

Elu

ent D

egassin

g D

evic

e D

EG

AS

SE

R® E

RC

-30

00α

serie

s ER

C-3

11

5α, 3

21

5α, 3

31

5α, 3

41

5α

HPLC System Trouble Shooting

87

Info

rmatio

nH

PLC

Syste

m T

rouble

Shootin

g

Common cause of abnormal chromatograms

Instable baseline values

······Main cause ······Frequent problem······Solution

······Main cause ······Frequent problem······SolutionNoise

Column Pressurenormal Malfuct ion of check valve

Deterioration of detector lamp

Temperature fluctuation

· Replace check valve· Clean up check valve

Replace lamp

· Warm up detector sufficiently· Shield detector from wind· Check column oven· Check detector temperature control

N ON O

N ON O

N ON O

N ON OInsufficient grounding Establish sufficient grounding

Malfunct ion of recorder Repair recorder

YESYESYES

YESYESYES

Baseline stable when pump stopped Monotonic drift

YESYESYES

Leakage observed

YESYESYES

Flow rate fluctuation

Tighten connections

START

Baseline stable when pump stopped Irregular noise

N ON O

N ON O

N ON O

YESYESYES

Spike noise found from begining

Problem solved by applying back pressure to column(*)

Shodex DEGAS used

YESYESYES

YESYESYES

YESYESYES

Noise shape leading

Bubbles in flow cell

YESYESYES

START

HPLC System Trouble Shooting

Insufficient grounding Establish sufficient grounding

Deterioration of detector lamp Replace detector lamp

N ON OInsufficient degassing eluent Use Shodex DEGAS

· Check column performance· Replace column

Reduce temperature of column oven and/or post-column reactioncoil

N ON OContamination of flow cell Wash flow cell

Repair detector

Malfunct ion of recorder

Air in column

Column temperature exceeding eluent boiling point

Spike noise

Repair recorder

Bubbles in flow cell Wash inside of detector

Contamination of flow cell Wash inside of detector

Insufficient eluent replacement

(with different solvent, salt or others)

Wash flow line of HPLC

Contamination of column· Wash column· Check column performance· Replace column

Pulsation of pump Use damper

Deterioration of eluent · Use eluent containing stabilizer (for THF, use THF containing BHT)

OK

(*) Do not apply back pressure to detector

88

AFpak Asahipak AXpak CLNpak CXpak EP GPC HIC

IC IEC MSpak ODSpak OHpak ORpak PROTEIN RSpak

Silica Silicapak STANDARD SUGAR USPpak WINE

[Series name]

AAB-894AAF-894AAM-894AAP-894AAV-894ABA-894ABT-894ACA-894ACB-894ACH-494ADS-894AED-894AGA-894AGE-894AGO-494AGT-894AHR-894A I A -894ALC-894ALS-894ANA-894AOV-894APA-894APB-894APD-894APG-894APH-894APR-894ARC-894AST-894AT-806MS, AT-GAWG-894

A

52, 53, 5452, 5352, 5352, 53

52, 53, 5422, 23, 52, 53

52, 5352, 53, 54

52, 5352, 53, 56, 57

52, 53, 5452, 5352, 5352, 5352, 5352, 5352, 5352, 53

52, 53, 5452, 53

52, 53, 5452, 5352, 5352, 5352, 5352, 5352, 5352, 5352, 53

52, 53, 5444, 4552, 53

DC-613, DC-GDE-213, DE-613DE-413DE-G, DE-SGDEAE3N-4TDEAE-825DM-614, DM-GDS-413, DS-613, DS-G

D

10, 118, 9, 48, 51

8, 9, 28, 48, 56, 578, 56, 58

16, 1716, 17, 58

88, 9

HFIP-603~607, HFIP-606M, HFIP-GHFIP-803~807, HFIP-806M, HFIP-LGHLDHLD-4GCHT-803~806, HT-806M, HT-G

H

44, 4544, 4550, 56

844, 45

E-411ERC-3115α, -3215α, -3315α, -3415αES-502C 7CES-502N 7CEV-200EV-2000, EV-GEV-2000 AC, EV-G AC

E

1286

14, 1516, 17

6060

60, 61

F-411, F-511, F-411/SFL-1FL-2

F

12, 1318, 2018, 20

GF-1G 7BGF-310 HQGF-310GF-4AGF-510 HQGF-710 HQGF-7M HQGOLF-413GS-220 HQGS-2G 7BGS-320 HQGS-320GS-520 HQGS-620 HQGS-620

G

3838, 39, 58, 59

48, 49, 50, 58, 5950, 5638, 3938, 3938, 39

5825, 36, 3725, 36, 56

36, 3748, 49, 50, 56, 57

3636

56, 57

C18MC18PC4P-50, C4P-50G 4A5C45C8C8P-50, C8P-50G 4ACARB-413, CARB-413LCARB-LFCDA-453 HQCDB-453 HQCDBS-453CDC-435 HQCD-GCM-8255CNCRX-853、CRX-G

C

12, 13, 5812, 13

6, 71212

6, 758, 5958, 5922, 2322, 2322, 2322, 23

2214, 15

1222, 23

INDEXColumns are listed under the product name excluding series name.

Instruments are indicated by bold letters.

Index b

y pro

duct n

am

eIn

de

x

89

I-524A, IA-G

I

20, 21 QA-825

Q

16, 17

JJ-50

J

8, 9, 48, 58, 59

LF-404, LF-604, LF-804, LF-G

L

46, 47

M-75MN-431

M

8224, 56, 57

5NHNH2P-50, NH2P-50GNH2P-LFNI-424, NI-GNN, NN-G5NPE 4D

N

1210, 11, 24, 25, 26, 48

1020, 21

8, 9, 28, 48, 58, 5912, 13

ODP2 HPODP-40ODP-50, ODP-GODP-51 4B

O

8, 9, 48, 496, 7, 516, 7, 48

50

P-421S, P-GP-82PAE-2000, PAE-GPAE-2000 AC, PAE-G ACPAE-800PAE-800 ACPH-814PK-2A, PK-4A5PYE 4D

P

28, 29, 488260

60, 6160

60, 6116, 17

50, 51, 56, 6712, 13

K-801~807, K-803L~807L, K-806M, K-GK-800DKC-811, KC-G, KC-LGKD-801~807, KD-806M, KD-GKF-400D, KF-600DKF-401HQ~404HQ, KF-405LHQ, KF-406LHQKF-601~607, KF-606MKF-801~807, KF-803L~807L, KF-806MKF-GKS-801~806, KS-GKS-807, KS-807GKW402.5-4F~KW405-4F, KW400G-4AKW-604SKW-802.5~804, KW-G

K

40, 4140, 46

28, 29, 5840, 4142, 46

42, 43, 48, 4942, 43

40, 41, 43, 4740, 42

24, 25, 26, 2724, 2732, 33

32, 51, 5732, 33

R-621, R-GRP18-413, RP18-415, RP18-613, RP18-GRI-101, RI-102, RI-104RI-201, RI-201H

R

188, 98584

SB401-4E~404-4E, SB400G-4ASB-802 HQ~806 HQ, SB-806M HQ, SB-GSC1011, SC1211, SC1821, SC-G, SC-LGSC1011-7FSH1011, SH1821, SH-GSH-75SI-90 4E, SI-50 4ESI-91 4C, SI-52 4ESI-90G5SILSL-105SM-105SP0810, SP-GSP-420NSP-80 4BSP-825SZ5532, SZ-G

S

34, 3534, 35, 56, 57

24, 25, 2624, 56

24, 25, 26, 5682

20, 2120, 58, 59

20, 5812, 13

8282

24, 25, 26, 2714, 15

5014, 1524, 25

T-521, T-G5TMS

T

18, 1912

VH-anion 4D, VH-anionG

V

20, 21

UT-802.5, UT-806M, UT-807, UT-G

U

44, 45

WA-624, WA-G

W

16, 17

Y-521, Y-521L, Y-GYK-421, YK-GYS-50, YS-G

Y

18, 1918, 1918, 19

Ind

ex

Index b

y pro

duct n

am

e

90

No. Title

1

2

3

No. Apprication Sample Column

1

Shodex GPC LF series columnsLinear calibration curves over awide molecular weight range

High performance reversed phase column; polymer-based column Asahipak ODP-40

Catecholamine, Alkaline medical compounds Asahipak ODP-40 Series

2Direct analysis of medical compounds in serum without sample pretreatment Propranolol, Medical compound for TDM, Haroperidol MSpak GF-310 series,

MSpak GS-320 series

7Multi-mode polymer based column with reversed phase and ionic interaction

Arsenic compounds, Water soluble Vitamins,Derivation of surfactants, Haroaceric acids

RSpak NN seriesRSpak JJ-50

8Ultra-high molecular weight and water-soluble polymer can be analyzed with Shodex SUGAR KS-807

Hyaruonic acid, Polyacrylamide SUGAR KS-807, KS-800 series

9Direct analysis of proteins and peptides in surfactants using surfactant removal column MSpak PK series.

Tryptic digest of Myoglobin, BSA, Surfactants MSpak PK series, MSpak GF-4A

3 Semi-micro GPC columns with high resolution Polystyrene, Additives GPC KF-400HQ series

4 Linear calibration curves by GPC LF-804 Phenol resin, Polystyrene GPC LF-804

10 Direct analysis of additives in polymer with SEC/MS IRGANOX, TINUVIN, BHT GPC KF-400HQ series

12 Column selection for proteins and peptides analysis Protein, Peptides, Surfactants SEC, Reversed phase, Ion exchangem Affinity columns

13 Analysis of Hyaluronic acid with ultra-high molecular weight in serum

Hyaruonic acid SUGAR KS-807, Asahipak GS-620HQ

14 Water-Soluble Vitamins Niacinamide, Vitamin B1, B2, B6, C Asahipak NH2P 50 4E,RSpak DE-413

19 New Micro column seriesMetal-binding peptides, Isopropyl methylphosphonate,Staphylococcal Enterotoxin B GS320A-M5D, GS520A-M5D

11 Concentration of the hydrophilic medical compounds existing with proteins Medical compounds MSpak PK series

20 New Micro column series;similar separation with standard column and high sensitivity

Metal combined Amino acid, Staphylococcal Enterotoxin B (toxic protein)

GS-320 HQ, GS320A-2E, GS320A-1E

24 Analaysis of amines and amides with RSpak DE seriesCholine, Ethylhomocholine, Acetylcholine, Catecholamines, Norepinephrine, Epinephrine, Dopamine, Formamide, Acetamide, Propionnamide

Rspak DE-613,DE-413,AFpak ACH-494

23Various application with Shodex Rspak DE series; high pH, 0% organic eluent

Benzocaine, Lidocaine, Tetracaine, Procainamaide, N-Acetylprocainamide, N-Propylprocainamide, Lactitol, meso-Erythritol, Hinokitiol, Oxine-Cu

Rspak DE series

5 New IC columns for suppressed ion chromatography lnorganic anions Organic acids IC SI-90 4E, SI-50 4E

15 Anion analysis with non-suppressor method Anions IC NI-424

30 New reversed phase column ODP2 HP Uracil, Caffein, barbital in BSA ODP2 HP series

32 High sensitive analysis of pyridylaminated saccharide pyridylaminated sugar chain, pyridylaminated saccharide Asahipak NH2P-50

31 New protein analysis column KW400 Blue dextran, γ-Globulin, Ovalbumin, Myoglobin, Uridine, Control serum KW400 series

33 New Cation Exchange column; DEAR3N-4T with nonporous gelConalbumin, Ovalbumin, Trypsin inhibitor, BSA, α Lactoalbumin, β Lactoglobulin IEC DEAE3N-4T

34 Oxyhalides and Cyan in Drinking waters Oxyhalides, Anion, Cyan ion (CN-), Cyanogen chloride(CNCl), Tap water

IC SI-52 4E, IC SI-91 4C, RSpak KC-811 6E

35 Shellfish Poisoning and pesticides Diarrhetic Shellfish Poisoning and fat-soluble poisoning, Ethoxyquin

RSpak DE413-2B, Asahipak ODP-50 4E

36 Suitable column for LC/MS application; ODP2 HP BSA, Barbital, Lidocaine, Chlorpheniramine, Propranolol,Diphenhydramine, Bupivacaine, Verapamil, Imipramine ODP2 HP -LCMS

16 SEC analysis of proteins with PROTEIN KW-800 series Proteins, Human serum PROTEIN KW-800 series

22 Online Removal of Surfactants in Protein Analysis BSA, Surfactants MSpak PK-2A, PROTEIN KW-604S

18 Analysis of quite small amount of sample with micro-column Metal-binding amino acids Shodex Micro Column series

21 Shodex Affinity column series Globulin Bovine γ-globulin Affinity column : AFpak series

17 Saccharides and sugar alcohols with Asahipak NH2P-50 column Saccharides, Sugar alcohols Asahipak NH2P-50

25Simultaneous analysis of monosaccharide and organic asid is possible with SUGAR SH1011

N-Acetylneuraminic acid(Sialic acid), Glucose, Mannose, Fucose, N-Acetyl-D-glucosamine, N-Acetyl-D-galactosamine,

SUGAR SH1011

26 ORpak CDBS-453; Chiral column for the analysis of drugs and their metabolites.

Chlorpheniramine, Monodesmethyl-chlorpheniramine, Didesmethyl-chlorpheniramine ORpak CDBS-453

27 Analysis of Chiral medicine Warfarin and its metabolites with CDBS-453. Warfarin, Lorazepam, Epinephrine, Valetamate ORpak CDBS-453

28 Cations Analysis column "IC YS-50" for Suppressor and Non-suppressor system Cations, Alkylamines, Transition metals ions IC YS-50, IC YK-41

29 Water-soluble ultra-high Molecular compounds can be analyzed with OHpak SB-807HQ Polyacrylamide, Hyaluronic acid OHpak SB-807HQ,

SB-806HQ

6 Food additives Sugars,Vitamin C,Organic acidsSUGAR SP0810, SC1011,Asahipak NH2P-50 4E,RSpak KC-811

Shodex NH2P-50 series columnsAnalysis of saccharides in food industry

Food Analysis with Shodex columnsSaccharides, Organic acids, Vitamins, Fatty acids and Amino acids

No. Title

4

5

6

Shodex IC YS-50 columnSimultaneous analysis of monovalent and divalent cations at a higher sensitivity

Shodex KW400 series columnsHigh performance and downsized column for protein analysis

Shodex ODP2 HP series columnsBetter retention of highly polar substances

Technical Notebook

Chromato News

*Contact Shodex or our distributors near you for these application information.

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al in

form

atio

n list

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rmatio

n

Technical information list

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Capture the Essence - Laserchrom · Capture the Essence HPLC Columns and Instruments 2008 – 2010....

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