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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Breeding and Producing

    Elite Seedlingsfor Jatropha Plantation

    Breeding and Producing

    Elite Seedlingsfor Jatropha Plantation

    Dr. HONG YanGeneral manager (Research and Operation)Email: [email protected]

    www.joil.com.sg

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    A small tree or large shrub originated from central America Widely introduced into tropical and subtropical countries It adapts well in marginal and degraded soils, can survive arid

    and semi arid conditions Traditional uses:

    Living fence and hedge plants Reclaiming eroded land Folk medicine Soap making, lighting and cooking fire Cake as a good fertilizer

    Holds great potential as a biodiesel feedstock plant

    Introduction to Jatropha curcas

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Feedstock AttributesAttribute Rapeseed Palm Soya JatrophaFit for EU SustainabilityCostAvailabilityGHG FootprintPerennialNon-FoodFreezing Point - 10 oC o10C - 2 oC - 2 oC*Jet Fuel

    X X X

    X X X

    X X X

    X X XXX

    X

    X X

    X X

    X

    X X

    X

    X

    XX

    X

    X

    Jatropha curcas , a good choice ofbiodiesel feedstock plant

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    JOil (S) Pte. Ltd.JOil (S) Pte. Ltd.

    A joint venture private limited company incorporated by TLL, TATAchemicals, Toyota Tsusho and other partners

    Headquartered in Singapore, the company will have its business invarious countries and regions suitable for jatropha plantation

    Members of Roundtable for Sustainable Biofuel (RSB) and Jatropha Alliance

    Core activities : R&D to improve Jatropha through traditional breeding and genetic

    modification Jatropha tissue culture technology Production and sale of elite Jatropha seedlings Consulting services for Jatropha plantations

    Has the exclusive commercial rights for jatropha R&D in TLL

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Jatrophatoday

    Positives Negatives

    J A T R O P H A

    T o x i c i t y i s s u e s

    U n c e r t a i n e c o n o m i c s d u e t o l o w p r o d u c t i v i t y

    R u r a l d e v e l o p m e n t

    C a n g r o w o n m a r g i n a l l a n d s ( f o o d v s . F u e l )

    H a r v e s t i n g & S t o r a g e i s s u e s

    N o n e d i b l e ( f o o d v s . F u e l )

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Jatropha Value Chain

    PlantingmaterialsPlanting

    materialsLand

    management

    Plantation &crop

    maintenance

    Harvesting Oil ExtractionBiodiesel

    Manufacturing

    7%7% 33%33% 32%32%

    7%7% 4%4% 19%19%

    Plantingmaterial

    plays a keyrole in

    success ofentire

    value chain

    Plantingmaterial

    plays a keyrole in

    success ofentire

    value chain

    Basis : Cost Allocation based on test plantations

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    What Biotechnology can do to Jatropha.. Developing good hybrids

    Accessions from all over the world, comparison of performance and selection

    of best performing accessions Controlled pollinations

    Marker assisted breeding using genetic markers

    Tissue culture propagation of elite hybrids Addresses uniformity

    Fast multiplication for large scale production

    Shortens breeding cycle

    Genetic modification (GM)

    Pathogen tolerance, higher seed yield, lower toxicity, higher oil

    content

    JOil focuses on all the three aspects

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Strategy I: Combining the best traits

    First generationvarieties :

    Variety I:

    #High number of flowers butmedium level of female flowers#Soft branch

    Variety II:

    #Higher female flower ratio#vigorous growth with strong branc

    x

    Second generationhybrids:

    #high number of flowers +high female flower ratio

    #stronger branches

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Strategy II: Introgression breeding throughinterspecies hybridization

    1.1 m

    One BC2F1 plant (~9 mth old)- Compact size with strong branches-Very high female flower ratio,-Flowering bunches are close-Clearly identifiable traits

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Strategy III: Improving local varieties

    Cross pollinationbetween our bestmaterial with localvarieties collected fromour markets

    To create hybrids ofhigher productivity, alsowith local adaptation

    Hybrid vigor foundbetween our best hybrid(SEA origin) and Indian,Chinese and Africancollections

    A hybrid with Indian heritage

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Strategy III: Improving local varieties

    A hybrid with African heritage

    A hybrid with Chinese heritage

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    How to provide plantation materials?

    Propagationmethods

    Advantages Disadvantages

    Seeds

    -Easy to carry aroundand storage

    -Cost effective

    -Variable performance in field due tosegregation

    -Potential inbreeding depression with pure line approach

    -Long breeding cycle for hybridapproach

    -loss of viability

    Stem cutting -Cost effective-Easier to practice

    -Slow and less efficient propagation

    -Weaker rooting

    Tissue culture(our choice)

    -High propagation ratio-True-to-type cloning

    -Strong rooting -Short breeding cycle

    -Technical demanding -Higher cost

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Oil proprietaryatropha curcas

    in vitro propagation

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Jatropha clones 4month after

    transplanting

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Jatropha tissue culturetechnology of JOil

    A leader in commercial scale production To avoid natural segregation in seed derived plants Fast, with high propagation ratio and wide

    applicability to various jatropha varieties

    Produces hundreds of thousands of true to typeclones from a single elite hybrid plant within a year

    Features of the phase 1 varieties Natural branching Early flowering and Good fruiting

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    TLL/JOil jatrophaExperimentStation inSingapore

    2009

    2010

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Global (Asia and Africa) Operations

    IndiaLab PuneTrials - Aurangabad

    ChinaLab Shanghai

    PhillipinesLab Davao

    KenyaLab NairobiTrial Kajiado

    ThailandLab Bangkok

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    JOil : Key Considerations

    Superior technology Reputed shareholders Committed management team Global operations

    Key success factors Leveraging on strong partners Performance backed products (through field trials, demo

    projects)

    Continuous Research & Development

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    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    How we can work togetherto make Jatropha viable

    Introduction of new varieties, collaboration in field trials Technical services:

    Due diligence services for existing Jatropha plantations Technical consulting for undertaking new Jatropha plantations Technical consulting for improving existing Jatropha plantations Seed quality traits analysis

    Licensing in, licensing out for new variety and technology OEM production and contract research

    Tissue culture facilities (purchase / lease / JV) Many more

    JOil presentation for JATROPHAWORLD 2010, Rotterdam

    Thank You!