GENUS:GENUS:BORDETELLABORDETELLA

Prepared by:Prepared by:Salma MahmoudSalma Mahmoud

Medical Important Spp:Medical Important Spp:

1-B. pertussis1-B. pertussis 2-B. parapertussis2-B. parapertussis 3-B. broncoseptica3-B. broncoseptica

Normal HabitatNormal Habitat

B. pertussis and B. parapertussis B. pertussis and B. parapertussis found in respiratory tract of found in respiratory tract of infected person.infected person.

B.proncoseptica is present in B.proncoseptica is present in respiratory tract of animal.respiratory tract of animal.

Bordetella pertussisBordetella pertussis

Clinical Infection : Clinical Infection : Whooping Whooping coughcough

EpidemiologyEpidemiology Humans only reservoirHumans only reservoir Occur primarily in non immune Occur primarily in non immune

childrenchildren Misdiagnosed as cold or flu Misdiagnosed as cold or flu

Bordetella pertussisBordetella pertussis

Morphology and Staining:Morphology and Staining:

Small Gram Gram negative Small Gram Gram negative Coccobacilli, Pleomorphic, non Coccobacilli, Pleomorphic, non motile, and non Sporing, motile, and non Sporing, Capsulated, it may show bi polar Capsulated, it may show bi polar Staining.Staining.

Culture Characteristic:Culture Characteristic: B. pertussis are strict aerobes, optimal B. pertussis are strict aerobes, optimal

temperature for growth is 35 c .temperature for growth is 35 c . Selective and enriched media such as:-Selective and enriched media such as:- Bordet Gengou Penicillin medium.Bordet Gengou Penicillin medium. Require blood, starch or charcoal for Require blood, starch or charcoal for

growthgrowth The growth is detected after 72 Hrs as The growth is detected after 72 Hrs as

greish, white colonies, with a shiny greish, white colonies, with a shiny surface and high convex shape, Mercury surface and high convex shape, Mercury drop.drop.

Sensitiviy to Physical agents:Sensitiviy to Physical agents: B. pertussis is Fragile, Killed by B. pertussis is Fragile, Killed by

heat at 55 C for 30 min.heat at 55 C for 30 min.

Serological Character: Serological Character: B. pertussis have 3 major antigen B. pertussis have 3 major antigen

( 1,2,and 3)( 1,2,and 3)

B. parapertussisB. parapertussis Cause Mild form of Whooping Cause Mild form of Whooping

Cough, differ from B. pertussis Cough, differ from B. pertussis from some biochemical tests and from some biochemical tests and Culture Characteristics, and also Culture Characteristics, and also differ serologically using Anti differ serologically using Anti sera. sera.

B. bronchisepticaB. bronchiseptica It’s Motile and grow on Ordinary It’s Motile and grow on Ordinary

media without addition of blood.media without addition of blood.

It’s may causes secondary It’s may causes secondary respiratory tract infection in respiratory tract infection in rodents, in human it’s important rodents, in human it’s important causes of Pneumonia in Immune causes of Pneumonia in Immune compromised Patient.compromised Patient.

Lab diagnosis of Lab diagnosis of Whooping coughWhooping cough

Specimen Collection: Specimen Collection: Per nasal Swab, Cultured immediately Per nasal Swab, Cultured immediately

on BGA or placed in transport media.on BGA or placed in transport media. Culture of B. pertussis :Culture of B. pertussis : Culture on selective enriched media Culture on selective enriched media

BGA in humid atmosphere .BGA in humid atmosphere . Colonial Morphology:Colonial Morphology: Appearing after 3-5 days incubated at Appearing after 3-5 days incubated at

37C .37C .

Differential Differential Characteristics of Characteristics of Bordetella SpeciesBordetella Species

Serological TestsSerological Tests Detection of Bacterial AntigensDetection of Bacterial Antigens Bordetella antigens in serum and Bordetella antigens in serum and

using specific antiserum using specific antiserum Immunofluorescence TechniqueImmunofluorescence Technique

Bacteria in nasopharyngeal Bacteria in nasopharyngeal secretions labelled with secretions labelled with fluorescein-conjugated antiserum fluorescein-conjugated antiserum > examined with ultraviolet > examined with ultraviolet microscopy.microscopy.

Detection of Bordetella AntibodyDetection of Bordetella Antibody Sera and nasopharyngeal Sera and nasopharyngeal

secretions examined for Abs secretions examined for Abs using Agglutination Tests or using Agglutination Tests or ELISAELISA

TreatmentTreatment Erythromycin is drug of Choice Erythromycin is drug of Choice

for Whooping Cough.for Whooping Cough.

CONTROLCONTROL Vaccination:Vaccination: Vaccination (DPT –diphtheria, pertussis, Vaccination (DPT –diphtheria, pertussis,

tetanus)tetanus) Safe and >90% effective.Safe and >90% effective. Adsorbed vaccine (i.e. with adjuvant).Adsorbed vaccine (i.e. with adjuvant). Contains all 3 agglutinins.Contains all 3 agglutinins. Minimum 3 doses.Minimum 3 doses. Minor adverse reactions:Minor adverse reactions: Erythema and local swelling and slight feverErythema and local swelling and slight fever Acellular pertussis vaccineAcellular pertussis vaccine