BMEN 423/621 Laser Microscopy
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BMEN 423/621 Laser Microscopy. Alvin T. Yeh [email protected] http://biomed.tamu.edu/tml. K. Konig J. Microsc. 200: 83-104 (2000). Microscopic Imaging. Light- vs. Dark-field imaging Stained vs. unlabeled Vital vs. fixed. Increasing use of Laser Microscopy. - PowerPoint PPT Presentation
Transcript of BMEN 423/621 Laser Microscopy
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K. Konig J. Microsc. 200: 83-104 (2000).
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Microscopic ImagingLight- vs. Dark-field imaging
Stained vs. unlabeledVital vs. fixed
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Increasing use of Laser Microscopy
W.R. Zipfel, R.M. Williams, W.W. Webb Nat. Biotechnol. 21(11): 1369-1377 (2003).
•commercialization of laser microscopy systems•increased technical savvy of biologists
•benefit to biomedical studies
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Technically Demanding
W.R. Zipfel, R.M. Williams, W.W. Webb Nat. Biotechnol. 21(11): 1369-1377 (2003).
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Laser-Tissue Interaction
K. Konig J. Microsc. 200: 83-104 (2000).
Does this schematic capture photophysics correctly?
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