Bloodborne Pathogens

Edgar Romo, REHSEnvironmental Health & Biosafety Specialist

Janette De La Rosa Ducut, Ed.D.Training Manager

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1. Access the Bloodborne Pathogens Standard2. Identify epidemiology and symptoms

3. Explain the modes of transmission4. Locate the Exposure Control Plan at UCR

5. Recognize tasks/activities

6. Identify methods of prevention

7. Decontaminate and dispose of PPE

Objectives

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8. Select proper PPE

9. Obtain, or decline, the Hep B vaccine10. Follow emergency procedures11. Take action in an exposure incident12. Complete post-exposure evaluation13. Recognize signs and labels

14. Manage biohazardous waste

15. Obtain answers to your questions

Objectives

4

Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Bloodborne Pathogens Standard

The StandardApply to all work where occupational exposure to bloodborne pathogens is reasonably anticipated View online at

http://ehs.ucr.edu/biosafety

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Bloodborne Pathogens Standard

Bloodborne PathogensMicroorganisms that are carried in blood

that can cause disease

HBV HIV

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Epidemiology and Symptoms

EpidemiologyPlan and evaluate strategies to prevent illness by studying how diseases occur.

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Modes of Transmission

Routes of exposure Ways toxins enter the body Inhalation Absorption

Injection Ingestion

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Modes of Transmission

Mucous Membranes

Injection Non-Intact Skin

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Modes of Transmission

Potentially infectious materialsTransmission of a biohazardous agent via body fluid

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Modes of Transmission

Potentially infectious materials

urinefeces

vomit

tears

sweat

sputum

nasal secretions

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Exposure Control Plan

Exposure Control Plan

Review online at http://ehs.ucr.edu/biosafety

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Exposure Control Plan

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Exposure Control Plan

Biological Use Authorization (BUA)Review the conditions / restrictions for use

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Tasks and Activities

Determine if your tasks, activities, or job description involves exposure and protect yourself.

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Tasks and Activities

Follow precautions when handling…Syringes, needles, or sharpsEmergency responseBody fluidsSpill clean-ups

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Tasks and Activities

Job DescriptionsPhysicians and NursesAthletic TrainersLifeguardsEmergency Responders (i.e., UCPD)

Laboratory workersPhlebotomistsBiohazardous waste workersCustodiansGroundskeepers

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Methods of Compliance

Methods ofCompliance

Universal PrecautionsHousekeepingEngineering ControlsWork PracticesPPE

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Methods of Compliance

Universal PrecautionsHandle as if items were known to be infectious

Wash hands and use protective barriers (i.e., PPE) for direct contact with blood and other potentially infectious materials.

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Methods of Compliance

HousekeepingDecontaminate with a 10% bleach solution(1 part bleach to 9 parts water) or other approved disinfectant for at least 15 minutes

Sanitize with 1:10 bleach solution

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Methods of Compliance

Engineering ControlsEliminate or reduce exposure through use or substitution of

machinery or equipment.

Dispose of sharps in a puncture-

proof container

Needleless System Use aerosols in a Biological Safety

Cabinet

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Methods of Compliance

Work Practices

Implement safe ways of performing your tasks

One-Hand Technique

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Methods of Compliance

HygieneAvoid contactCheck for open wounds Wash hands

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EatingDrinkingSmoking…in the laboratory

Methods of Compliance

NO

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Methods of Compliance

PPECheck for defects, remove properly, and replace immediately.

Do not reuse.

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

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PPESelection

PPE

Gloves: Latex or NitrileEyes: Safety Glasses,

Goggles or Face Shield

Body: Lab CoatFeet: Closed-Toe Shoes

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

PPEDecontamination and Disposal

After usingDecontaminate (i.e., wash hands after removing gloves)

Dispose of in biohazard containers

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Properly remove PPE

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Hepatitis B Vaccination

Hep B Vaccine

Participate in a free series of three shots (80-95% effective at preventing infection)

Hepatitis B Vaccination

Hep B Vaccine

You may elect to decline the vaccine

Hepatitis B Vaccination

Hep B Vaccine

Receive the vaccine, OR Complete the Declination Form

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Emergency Procedures

In Case of an AccidentObtain medical care and report the injury or illness

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Emergency Procedures

SpillsIsolate, decontaminate, and wash hands

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Emergency Procedures

Review the Emergency Procedures online at http://ehs.ucr.edu/emergency/procedures

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Exposures

ExposureContact between eyes, mouth, mucous membranes, skin cuts, or abrasions

Inju

ry R

epo

rt F

orm

Exposures

NotificationYou complete an Injury Report Form

UCR records exposures on a Sharps Injury Log.

Sh

arp

s In

jury

Lo

g

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Exposures

EmergencyProcedures

Wash skin immediately.Flush eyes with water for 15 minutes.Call 911 for help.Notify supervisor and EH&S.

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Post-ExposureEvaluation and Follow-Up

Medical EvaluationRoutes of exposureSource individualBlood testingProphylaxis (if applicable)

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Signs and Labels

Biohazard SymbolFlourescent orange or orange-red in color, with contrasting lettering

Signs and Labels

Affix biohazard label

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Waste

Biohazardous WasteBiologically contaminated waste that could potentially cause harm to human health, animal health, or the environment.

Examples: cell cultures, transgenic plants, recombinant DNA, and waste from production of bacteria / virus / fungi / parasites.

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Waste

LiquidWaste

The three types of biohazardous waste generated on campus are contaminated sharps, red bag waste, and liquid waste.

ContaminatedSharps

Red BagWaste

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Contaminated Sharps

WasteContaminated Sharps

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Label with the word “Biohazard” and the biohazardous symbol

WasteContaminated Sharps

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Store in approved sharps container

WasteContaminated Sharps

LabelsLabels

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Store until the container reaches the fill line.

WasteContaminated Sharps

Do not exceed fill line

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Decontamination is not necessary(unless you plan to dispose as physically hazardous waste)

WasteContaminated Sharps

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Dispose within the appropriate time after generation

WasteContaminated Sharps

Within 90 daysif stored below 0˚C

Within 7 daysif stored above 0˚C

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Submit a waste pickup request online by visiting www.ehs.ucr.edu and clicking on “Waste Pickup”

WasteContaminated Sharps

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WasteContaminated Sharps

Click on the link “Biohazardous or Radioactive Waste”

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WasteContaminated Sharps

Enter information and click on “Submit”

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WasteRed Bag Waste

Red Bag Waste

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Label with:1. “Biohazard” word2. Symbol3. Building

4. Room Number

WasteRed Bag Waste

Hinderaker 4108

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Label with:1. “Biohazard” word2. Symbol3. Building

4. Room Number

WasteRed Bag Waste

Hinderaker 4108

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WasteRed Bag Waste

Store in a labeled and leak-resistant secondary container

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WasteRed Bag Waste

Close containers when not in use.

Labels

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Treat within the appropriate time after generation by autoclaving

WasteRed Bag Waste

Within 90 daysif stored below 0˚C

Within 7 daysif stored above 0˚C

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Red Bag WasteWaste

Autoclave tape

Place indicator tape on the red bag

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Red Bag WasteWaste

Autoclave

Follow safe autoclave procedures

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Red Bag WasteWaste

Dispose of autoclaved waste in the building dumpster.

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Red Bag WasteWaste

Submit a waste pickup request online when autoclaving is not possible

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Go to www.ehs.ucr.edu and click on “Waste Pickup”

WasteContaminated Sharps

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WasteContaminated Sharps

Click on the link “Biohazardous or Radioactive Waste”

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WasteContaminated Sharps

Enter information and click on “Submit”

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LiquidWaste

WasteLiquid Waste

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Label with:1. “Biohazard” word2. Symbol

WasteLiquid Waste

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WasteLiquid Waste

Store in a clear, non-breakable, container with positive closure

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Treat within the appropriate time after generation by disinfecting

WasteLiquid Waste

Within 90 daysif stored below 0˚C

Within 7 daysif stored above 0˚C

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Mix 1 part bleach to 9 parts water

WasteLiquid Waste

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9

8

7

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5

4

3

2

1

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Add to liquid waste and wait for 30 minutes

WasteLiquid Waste

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Pour down drain(only if decontaminated)

WasteLiquid Waste

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Waste

Mixed waste must be treated in the following order: 1. Radioactive 2. Chemical 3. Biohazardous

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Waste

Mixed WasteWaste that contains radioactive, chemical, and biohazardous components

32P

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Waste

Mixed WasteWaste that contains radioactive and biohazardous components32P

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Waste

Mixed WasteWaste that contains chemical and biohazardous components.

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Topics

1. Bloodborne Pathogens Standard

2. Epidemiology and Symptoms

3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of

Compliance7. PPE

(Selection)

8. PPE (Decontamination and Disposal)

9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure

Evaluation 13. Signs and Labels14. Waste15. Questions and Answers

Questions and Answers

Biosafety Officer

Email ehs@ucr.edu Call (951) 827-5528Visit http://ehs.ucr.edu/biosafety

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1. Access the Bloodborne Pathogens Standard2. Identify epidemiology and symptoms

3. Explain the modes of transmission4. Locate the Exposure Control Plan at UCR

5. Recognize tasks/activities

6. Identify methods of prevention

7. Decontaminate and dispose of PPE

Summary

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8. Select proper PPE

9. Obtain, or decline, the Hep B vaccine10. Follow emergency procedures11. Take action in an exposure incident12. Complete post-exposure evaluation13. Recognize signs and labels

14. Manage biohazardous waste

15. Obtain answers to your questions

Objectives

117

For more information

ehs.ucr.edu

951 827 5528

ehs@ucr.edu