Biohit Catalogue 2010

Liquid HandlingProduct Catalogue

2 Biohit Liquid Handling Products

The hummingbird

The sensitive and precise qualities of the hummingbird

symbolise Biohit’s product groups of liquid handling,

diagnostic products, and analysis systems.

Biohit’s liquid handling products based on the company’s

innovations and technologies are characterised by

accuracy, precision and quality, as well as ergonomics

and safety.

3Biohit Liquid Handling Products

Catalogue Contents

Pipettes 5 Products & Innovations 6Choosing the Right Pipette 7Mechanical PipettesmLINE Single & Multichannel Pipettes 8Proline Plus Single & Multichannel Pipettes 14Proline Plus Single-Channel Fixed Volume Pipettes 17Proline Single & Multichannel Pipettes 20Proline Single-Channel Fixed Volume Pipettes 23Electronic PipetteseLINE Single & Multichannel Pipettes 24Proline Single & Multichannel Pipettes 28 ePET Single & Multichannel Pipettes 32 Pipetting Modes 35 Safe-Cone Filters 36

Optifit Pipette Tips 37Non-Filtered Tips & SafetySpace™ Filter Tips 40Packaging Options 41Tip Specifications 42Pipette - Tip Compatibility Charts 44Premium Tip Quality 46

Dispensers & Tips 47eLINE Lite & Pro Dispenser 48 Mechanical Stepper 51 Dispenser Tips 52

Accessories 53Ergomate 54 Reagent Vessel 55 Proline Biocontrol 55 Pipette Stands 56

Maxi-Volume Liquid Handling 57Midi Plus 59Midi Pro 60Biofiller 61 Prospenser Bottle Top Dispenser 62Prospenser Bottle Top Dispenser, Basic 63Biotrate Digital Burette 64

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Custom-Made Solutions 65OEM Solutions 66rLINE Robotic Dispensing Module 68

Pipetting Academy 69Pipetting Academy - the Concept 70 Ergonomics 74Safety in Pipetting 75Accuracy & Precision 76Pipetting Preparations, Pipetting Action & Other Cautions 77

Pipette Calibration & Maintenance 79 Service Providers 81 Preventive Maintenance & Repair Service 82Calibration & Accredited Calibration Services 84Quanta - Service & Calibration Software 86 Pipette Decontamination Procedure 90 Autoclaving Instructions, Troubleshooting Guide 91

About Biohit 92

Quality Standards 93

Literature 94

Brochures 95

Pipettes/

Pipettes/


Pipettes

Enjoy your work!

Pipettes

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Founded in Finland in 1988, Biohit has established its position in the world market with its

innovative, high technology liquid handling products such as pipettes and pipette tips. The

majority of the electronic pipettes used in laboratories worldwide are developed by Biohit.

Combining performance, ergonomics and experience we are able to provide safe, accu-

rate and reproducible pipetting in the most demanding liquid handling applications.

Outstanding qualityWe can guarantee the accuracy and precision of our pipettes (see page 76), and help you optimize your re-sults, when you use them together with our Optifit tips (see pages 37-46).

Maximum comfortEver since we developed the first Proline pipette, we have aimed at improving ergonomics of pipette design and usability. Because we wish to reduce the risk of upper limb disorders, such as RSI, and let you enjoy your work. (see pages 69-74)

Enhanced safetySafety is not only about ergonomics and high-quality tools but also about protecting the tools and the sam-ple from contamination in order to obtain accurate and reliable results. (see pages 75-78)

Think economical, think ecologicalCost-efficient product features, service contracts and low lifetime costs of our products help you save money and get a high return on investment. We also aim to develop and manufacture products that will cause as little environmental loading as possible throughout their entire life cycles.

We offer more than just pipettes

Extended life-time We extend the life-time of our products trough our pro-fessional maintenance and calibration services that are provided by our certified service centres worldwide (see pages 79-91).

Sharing our knowledge and experience with youAfter more than 20 years in liquid handling, we firmly be-lieve we have gained quite a lot of experience and knowl-edge in the field. Pipetting Academy is the tool for us to help you learn ergonomics, proper tool selections and how to get the most accurate results. (see pages 69-73).

™Biohit CertifiedService Center

Innovating for Health since 1988

Enjoy your work!

Pipettes/

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Pipettes

Choosing the right pipette - selection guides

1 1000 µl / 1- channel model2 Available as accessories

Notes: 1 (dd) Available on all models exept the 1200 µl multichannel models,

2 (rP) Available on all models exept the 1200 and 5000 µl models,

3 (Sd) Only on the 1200 and 5000 µl models, 4 (Pm) Available in 1-ch models only, 5 (SA) Only on the 1200 µl models. 6 Except multichannel 1200 µl models

Mechanical Pipettes (page 8) (page 14) (page 20)

Functions mLINE® Proline®

PlusProline®

Fully autoclavable • •

Optiload all

models

multichannels

only

Safe-Cone filters • • •

Filter ejector •

Thermal insulation • •

Volume locking • click stops click stops

Pipetting force1 12 N 15 N 20 N

Weight1 77 g 82 g 84 g

Colour coding on display

• •

Colour coding caps • •2

ID tags •

Biohit 6 place Car-ousel Stand

• • •

Proline 5 place carousel stand

•

Biohit Linear Stand • • •

Pipette holder • •2 •

Warranty 3 years 3 years 3 years

CE/IVD marked • • •

Starter Kits avail-able

• •

Electronic Pipettes (page 24) (page 28) (page 32)

Functions eLINE® Proline® ePET®

Pipetting (P) • • •

Multiple Dispens-ing (d)

• • •

Diluting (dd) • •1 •

Reverse Pipetting

(rP)• •2

Sequential Dis-pensing (Sd)

• •3

Manual Pipetting

(Pm)•4

Automated Dis-pensing (Ad)

•

Multi Aspirating

(SA)• •5

Memory places for favourite programs

•

Post Delivery Mix-ing in P and dd

• • •

Speed Selection 9 speeds 5 speeds 5 speeds

Manual Tip Ejection • •

Electronic Tip Ejec-tion

•

Autoclavable lower part

•6

Low battery indica-tor

•

Single point cali-bration

• •

Optiload multichannels

only

Direct charging with AC-adaptor

Optional Included

Charging Stand (Holds 1 pipette)

• •

Charging Carousel (Holds 4 pipettes)

• •

Linear Stand • •

Warranty 2 years 2 years 2 years

CE/IVD marked • • •

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Effortless accuracy – meet the mLINE® family!Biohit’s most advanced mechanical pipette family – the mLINE® – offers a unique

selection of innovative & patented features for manual pipetting. Excellent performance,

smooth ergonomics and perfect safety makes the mLINE. It is comfortable to use, hour

after hour, day after day – without the risk of strains (RSI). Enjoy your work. With mLINE

pipettes you can. mLINE pipettes cover the full volume range of 0.1 µl to 10 ml.

Fully autoclavable

IVD

Effortless Tip Loading &

Eject

ion

Pipettes/

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Pipettes

Cat. No. Channels VolumeRange (µl)

Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)

Safe-Cone FiltersStandard Plus

Compatible Tip ID

725010 1 0.002 31.50.3

1.30 %2.40 %

10.00 %

0.80 %1.60 %6.00 %

- - A, B, L, M

725020 1 0.01 10 5 1

0.5

1.00 %1.50 %2.50 %

5.00 %

0.60 %1.00 %1.50 %4.00 %

- -

A, B, L, M

725030 1 0.02 20102

0.90 %1.20 %3.00 %

0.40 %1.00 %2.00 %

721014 - C, N

725050 1 0.10 1005010

0.80 %1.00 %2.00 %

0.15 %0.40 %1.00 %

721008 721018 C, D, O

725060 1 0.20 20010020

0.60 %0.80 %2.00 %

0.15 %0.30 %0.80 %

721007 721017 C, D, P, Q

725070 1 1.00 1000500100

0.60 %0.60 %1.00 %

0.20 %0.20 %0.40 %

721006 721016 E, G, S

725080 1 10.0 50002500500

0.50 %0.60 %2.00 %

0.20 %0.30 %0.60 %

721005 721015 J

725090 1 20.0 1000050001000

0.60 %1.20 %3.00 %

0.20 %0.30 %0.60 %

721005 721015 U

2-20 µl

0.5-10 µl

10-100 µl

20-200 µl

100-1000 µl

500-5000 µl

mLINE Mechanical Single-Channel Performance specifications and ordering details

The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice.

5 colour coding caps

Fast and light, stepless volume setting with small increments

Thumb activated filter ejection (page 9)

Ergonomic finger support, no need to squeeze the pipetteThermal insulation of the internal

components to improve accuracy

Clear and easy-to-read display with big numbers

Locking mechanism preventing accidential volume changes during pipetting

Volume range colour coding for easy selection of the right Biohit tip

Easy cleaning and maintenance without any opening tool

Improved tip fitting

All models > 10 µl offer replaceable Safe-Cone Filters and unique filter ejection to prevent internal contamination and damage

Fully autoclavable

0.1-3 µl


Eject

ion

1- 10ml

What’s the difference?

0.3/1.2 KG

0.5/1.5 KG

0.9/2.0 KG

Comparison of the pipetting/blow-out forces needed. See page 7 for the selection guide.

mLINE Proline Plus Proline

FINAS Accredited Calibration

Certificate (ISO 17025) available for new pipettes – see page 85 for details

™

Pipetting Academy on page 69

Recommended by Health & Safety Officers

Tips on pages 37-46

Filters on page 36

Stand options available on page 56

Ergomate on page 54

Ergonomics on page 74

Pipette selection guide on page 7

Autoclaving instructions on page 91

e

• PreventsRSIandimprovespipettingresults

– through the exceptionally light pipetting forces and ergonomic design

• Perfecttipsealing – with Optiload tip loading mechanism

• Contaminationfreepipetting – with Safe-Cone Filters

• Easiestpipettetomaintain – only three parts to disassemble

• Highlydurable,long-lasting – materials highly chemically and UV resistant – robust mechanics through innovative spring design

mLINE® - Recommended by health and safety officers around the globe

Pipettes/


Pipettes/


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

725120 8-channel 0.01 10 5 1

1.50 %2.50 %4.00 %

1.00 %2.50 %4.00 %

- - A, B, L, M

725130 8-channel 0.10 1005010

0.70 %1.00 %3.00 %

0.25 %0.70 %1.50 %

721008 721018 C, D, O

725140 8-channel 0.20 30015030

0.60 %1.00 %2.00 %

0.25 %0.50 %1.00 %

721007 721017 D, Q

725220 12-channel 0.01 10 5 1

1.50 %2.50 %4.00 %

1.00 %2.50 %4.00 %

- - A, B, L, M

725230 12-channel 0.10 1005010

0.70 %1.00 %3.00 %

0.25 %0.70 %1.50 %

721008 721018 C, D, O

725240 12-channel 0.20 30015030

0.60 %1.00 %2.00 %

0.25 %0.50 %1.00 %

721007 721017 D, Q

mLINE Mechanical Multichannel Performance specifications and ordering details


0.5-10 µl

5-100 µl

30-300 µl

0.5-10 µl

5-100 µl

30-300 µl

Fully autoclavable

Colour coding caps

Thumb activated filter ejectionLight plunger button action

Clear and easy-to-read display with big numbers and small increments

Locking mechanism preventing accidental volume changes during pipettingRotating dispensing head for

optimal pipetting convenience

Optiload tip loading mechanism for perfect tip sealing and light tip ejection

All models > 10 µl offer replaceable Safe-Cone Filters and unique filter ejection to prevent internal contamination and damage

Ergonomic finger support and ID tag for personal identification

Individual piston / tip cone assemblies for easy maintenance


Eject

ion

eNEW! Ergomate on page 54

Tips on pages 37-46

Filters on page 36


Ergomate on page 54




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Pipettes

Pipetting force comparison of mLINE (1000 µl model) and other major pipette brands. The mLINE provides significantly lighter pipetting force than other mechanical pipettes.

e Ergonomics on page 74

Filters on page 36

Unique protection with Safe-Cone Filters

To prevent contamina-

tion of the pipette and the

sample, the mLINE pipettes

feature replaceable Safe-

Cone Filters. The unique

Safe-Cone Filter ejection of

mLINE means that the fil-

ters can be easily and safely

removed by pressing the

plunger button. Safe-Cone

Filters are available for both

single- and multichannel

models > 10 µl.

Optiload tip loading and perfect tip sealingAll mLINE pipettes are equipped with spring loaded tip

cones – the Optiload mechanism developed

by Biohit, that

• Allows tips to be loaded with constant force

• Enables even and safe tip sealing to the tip cone(s)

with minimum force

• Makes tip ejection light and easy

• Is an advantage in multichannel models, in

particular, where even tip sealing is essential.

The mLINE pipette has a patented spring mechanism (plunger

technology) resulting in the exceptionally light pipetting forces.

This together with light tip ejection force and ergonomic design

of the handle reduces the risk of Repetitive Strain Injury (RSI).

Unique spring mechanism for lighter pipetting

“The qualities that really convinced me to switch toBiohitmLINEareitslightweight,lowestpipettingandtipejectionforces,easytoreaddisplay,lockingmechanismto prevent accidental volume changes and finally, itscompatibilitytotipscurrentlyusedatourcompany.”

RichardP.Roque, Preclinical Development,

a Seattle based biotechnology company, USA

No rocking needed


Eject

ion

Conventional pipettes17–43 N

Biohit mLINE

12 N

Compression spring

mechanism

Tension spring mechanism

Conventional pipettes use compression spring mechanism

resulting in heavy pipetting force. The tension spring mechanism

used in Biohit mLINE pipettes makes pipetting force almost 4

times lighter. Moreover, thanks to the unique mechanism the

starting force is always constant regardless of the volume.

UNIQUELYBIOHIT!

Force to the 2nd stop Force to the 1st stop

N50

45

40

35

30

25

20

15

10

5

Biohit mLINE Competitor Pipette A

Competitor Pipette B

Competitor Pipette C

Competitor Pipette D

Competitor Pipette E

Measurements have been carried out in Biohit R&D development by using force meas-urement equipment Mecmesin Imperial 2500

Pipettes/


Pipettes/

Cat. No. Item

725600 Carousel Stand for 6 pipettes

725610 Pipette Holder for one pipette

725620 Linear Stand

726203 Calibration tool/Tube opener

The mLINE should, like any other pipette, be stored in an

upright position. There are several different stand options for

mLINE:

• Handy holder that is included with each mLINE pipette

• Carousel stand for six pipettes

• Stylish linear stand that allows the pipette to be placed

on both sides of the stand

Easy maintenance and calibrationThe mLINE can be easily calibrated using the elegant

calibration tool. The tool also acts as a tube cap opener.

The mLINE has been designed for easy cleaning and

maintenance without any opening tool. Only 3 parts need to

be cleaned.

Stands & Accessories

Pipettes/

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Pipettes

Biohit mLINE Starter Kit 1 - Cat. No. 725651

• mLINE 0.5-10 µl• mLINE 10-100 µl• mLINE 100-1000 µl• Pipette Holder x 3• Colour Coding Caps • Biohit Pen

• Biohit Tip 0.2 –10 µl, Single Tray• Biohit Tip 0.5-300 µl, Single Tray• Biohit Tip 10-1000 µl, Single Tray• Calibration Tool/Tube Opener• Literature

Biohit mLINE Starter Kit offers an opportunity to test and get

started with mLINE. You can choose between two Starter Kits

with three single-channel mLINE pipettes. Both kits also include

Get started with mLINE Starter Kit!

Starter Kit

Biohit mLINE Starter Kit 2 - Cat. No. 725652

• mLINE 2-20 µl• mLINE 20-200 µl• mLINE 100-1000 µl• Pipette Holder x 3• Colour Coding Caps • Biohit Pen

• Biohit Tip 0.5-300 µl, Single Tray x 2• Biohit Tip 10-1000 µl, Single Tray• Calibration Tool/Tube Opener• Literature

colour coded tip racks for every pipette and many useful

accessories such as pipette holder, colour coding caps

and a calibration tool, which also acts as a tube opener.

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Pipettes/

Durable and dependable – Proline® PlusIn the new Proline® Plus pipette, Biohit has combined the basic functionalities of the

traditional Proline® mechanical with new, state-of-the-art pipette design and technology.

Particular emphasis has been put on safety, ergonomics, and efficiency in pipetting, as

well as on the high quality of the device. Proline Plus pipettes cover the full volume range

of 0.1µl-10ml, in both adjustable and fixed volume models.

Fully autoclavable

IVD


Eject

ion

Proline® Plus

Pipettes/

Pipettes/


Pipettes

Cat. No. Channels Volume range(µl)

Increment (µl)

Test volume (µl)

Inacc. (%)

Impr.(%)


Compatible Tip ID

728010 1 0.002 3.01.50.3

1.30 %2.40 %

10.00 %

0.80 %1.60 %6.00 %

- - A, B, L, M

728020 1 0.01 10 5 1

0.5

1.00 %1.50 %2.50 % 5.00 %

0.60 %1.00 %1.50 %4.00 %

- -

A, B, L, M

728030 1 0.02 20102

0.90 %1.20 %3.00 %

0.40 %1.00 %2.00 %

721014 - C, N

728050 1 0.10 1005010

0.80 %1.00 %2.00 %

0.15 %0.40 %1.00 %

721008 721018 C, D, O

728060 1 0.20 20010020

0.60 %0.80 %2.00 %

0.15 %0.30 %0.80 %

721007 721017 C, D, P, Q

728070 1 1.00 1000500100

0.60 %0.60 %1.00 %

0.20 %0.20 %0.40 %

721006 721016 E, G, S

728080 1 10.0 50002500500

0.50 %0.60 %2.00 %

0.20 %0.30 %0.60 %

721005 721015 J

728090 1 20.0 10000 50001000

0.60 %1.20 %3.00 %

0.20 %0.30 %0.60 %

721005 721015 U

2-20 µl

0.5-10 µl

10-100 µl

20-200 µl

100-1000 µl

500-5000 µl

Proline Plus Mechanical Single channel Performance Specifications and Ordering Details


0.1-3 µl



1-10 ml

Plus Is More!Volume settingwith click stops

Suitable for both left andright-handed users

Thermal insulation ofthe internal componentsensures that the warmth of the hand does not affect the pipetting results during long pipetting series

Good chemical &UV resistance

Colour coding ondisplay corresponds tothe colour coding of tiptrays

Fits the most commonly usedlab tubes thanks to the slimtip ejector collar

Safe-Cone Filters to prevent contamination

Robust design


0.3/1.2 KG

0.5/1.5 KG

0.9/2.0 KG

Comparison of the pipetting/blow-out forces needed. See page 7 for selection guide.

mLINE Proline Plus Proline

Fully autoclavable

e ™


Tips on pages 37-46

Filters on page 36


Ergomate on page 54




Proline Plus – the classic Biohit Proline reborn

• Robust design

• Good UV resistance

• Excellent chemical resistance

• Small increments

• Reduced pipetting and tip ejection force

• Fully autoclavable

Pipettes/


Pipettes/

Cat. No. Channels Volume range (µl)

Increment (µl)

Test volume (µl)

Inacc.(%)

Impr (%) Safe-cone filters Standard Plus

Compatible Tip ID

728120 8 0.01 10 5 1

1.50 %2.50 %4.00 %

1.00 %2.50 %4.00 %

- - A, B, L, M

728130 8 0.10 1005010

0.70 %1.00 %3.00 %

0.25 %0.70 %1.50 %

721008 721018 C, D, O

728140 8 0.20 30015030

0.60 %1.00 %2.00 %

0.25 %0.50 %1.00 %

721007 721017 D, Q

728220 12 0.01 10 5 1

1.50 %2.50 %4.00 %

1.00 %2.50 %4.00 %

- - A, B, L, M

728230 12 0.10 1005010

0.70 %1.00 %3.00 %

0.25 %0.70 %1.50 %

721008 721018 C, D, O

728240 12 0.20 30015030

0.60 %1.00 %2.00 %

0.25 %0.50 %1.00 %

721007 721017 D, Q

Proline Plus Mechanical Multichannel Performance specifications and ordering details


0.5-10 µl

10-100 µl

30-300 µl

0.5-10 µl

10-100 µl

30-300 µl

Fully autoclavable

Volume settingwith click stops

8- and 12-channel pipettes available

Robust design

Good chemical &UV resistance

Unique Optiload tip loading for easy tip loading and ejection, as well as excellent tip sealing in multichannel models

Suitable for both left andright-handed users

Thermal insulation ofthe internal componentsensures that the warmth ofthe hand does not affectthe pipetting resultsduring long pipettingseries

Safe-Cone Filters to prevent contamination


Optiload tip loading and perfect tip sealingAll Proline Plus multichannel pipettes are equipped

with unique spring loaded tip cones – the Optiload

mechanism developed by Biohit, that:

• Allows tips to be loaded with constant force

• Enables even and safe tip sealing to the tip

cones with minimum force

• Makes tip ejection light and easy

• Is an advantage in multichannel models,

where even tip sealing is essential


Eject

ion

NEW! Ergomate on page 54

Pipettes/

Pipettes/


Pipettes

Cat. No. Channels Volume range (µl)

Increment (µl)

Test volume (µl)

Inacc.(%)

Impr.(%)

Safe-cone filtersStandard Plus

Compatible Tip ID

728515 1 - 5 1.30 % 1.20 % - - A, B, L, M

728520 1 - 10 0.80 % 0.80 % - - A, B, L, M

728530 1 - 20 0.60 % 0.50 % 721014 - C, N

728535 1 - 25 0.50 % 0.30 % 721008 721018 C, O

728545 1 - 50 0.50 % 0.30 % 721008 721018 C, O

728550 1 - 100 0.50 % 0.30 % 721008 721018 C, D, O

728560 1 - 200 0.40 % 0.20 % 721007 721017 C, D, P, Q

728565 1 - 250 0.40 % 0.20 % 721006 721016 E, G, R, S

728567 1 - 500 0.30 % 0.20 % 721006 721016 E, G, R, S

728570 1 - 1000 0.30 % 0.20 % 721006 721016 E, G, S

728575 1 - 2000 0.30 % 0.15 % 721005 721015 J

728580 1 - 5000 0.30 % 0.15 % 721005 721015 J

728590 1 - 10000 0.60 % 0.20 % 721005 721015 U

10 ml

5000 µl

2000 µl

1000 µl

Proline Plus Single-Channel FIXED Volume Performance specifications and ordering details



The slim tip cone design fits also narrow laboratory tubes.

A wide range of fixed volume models available

Fully autoclavable

500 µl

250 µl

200 µl

100 µl

50 µl

25 µl

20 µl

10 µl

5 µl

e™


Tips on pages 37-46

Filters on page 36


Ergomate on page 54




Pipettes/


Pipettes/

Prevent contamination with Safe-Cone Filters

Proline Plus features unique Safe-Cone Filters that:

• Prevent fluids and liquid vapours from reaching the internal components of the pipette • Help you prevent contamination of your pipette and sample in your everyday work • Prolong the pipette life-time • Are replaceable • Can be removed by using convenient forceps • Are available for all models > 10 µl

Basic calibration and maintenance made easy

• No opening tools are needed

• Only 3 parts need to be cleaned

– piston, tip cone and tip ejector collar

• Calibration is easy with the tool included in the package

• If you you wish to leave it to a professional, Biohit offers

complete maintenance, repair and calibration services

Cat. No. Item

725600 Biohit Carousel Stand for 6 pipettes

725610 Biohit Pipette Holder for one Proline Plus/mLINE pipette

725620 Linear Stand for all Biohit pipettes

726203 Calibration tool/Tube opener

The Proline Plus pipette should be stored in an upright position.

There are several different stand options:

• Carousel stand for six pipettes

• Stylish linear stand that allows the pipette to be placed

on both sides of the stand

• A handy holder that is available as an accessory


Pipettes/

Pipettes/


Pipettes

Biohit Proline Plus Starter Kit offers an opportunity to test and get

started with Proline Plus. You can choose between four Starter

Kits with two or three single-channel Proline Plus pipettes. All of

Proline® Plus Starter Kit

Biohit Proline Plus Starter Kit 3 - Cat. No. 728652

• Proline Plus 2 - 20 µl• Proline Plus 20 - 200 µl• Proline Plus 100 - 1000 µl• Pipette Holder x 3

• 3 single-tray tip boxes• Calibration Tool/Tube Opener• Literature


• Proline Plus 500-5000 µl• Proline Plus 1000-10 000 µl• Pipette Holder x 2

• 2 bulk tip boxes• Calibration Tool/Tube Opener • Literature


• Proline Plus 0.1 - 3 µl • Proline Plus 0.5 -10 µl • Pipette Holder x 2



• Proline Plus 0.5 - 10 µl• Proline Plus 10 - 100 µl• Proline Plus 100-1000 µl• Pipette Holder x 3


the kits include many useful accessories such as a pipette holder,

and a calibration tool, which also acts as a tube opener, as well

as literature.

Get started with Proline® Plus Starter Kit!

Pipettes/


Pipettes/

The original Biohit mechanical pipette – Proline®

Proline

Almost 20 years in the top of the world of pipettes, designed and manufactured by

Biohit. In that time Proline® pipettes have become a synonym for high quality. Working

with Proline, you will get many years of reliable service. The Proline pipettes cover the

volume range 0.1 µl to 5000 µl with 8 different adjustable single-channel and 8 adjustable

multichannel models. Proline is also available in fixed volume single-channel models.

IVD

Pipettes/

Pipettes/


Pipettes

The original Biohit mechanical pipette – Proline®

Proline Mechanical Single-Channel Performance specifications and ordering details



Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

720005 1 0.05 2.51.250.25

2.50 %3.00 %

12.00 %

2.00 %3.00 %6.00 %

- -

A, B, L, M

720000 1 0.10 1051

1.00 %1.50 %2.50 %

0.80 %1.50 %1.50 %

- -

A, B, C, L, M, N

720080 1 0.50 20102

0.90 %1.20 %3.00 %

0.40 %1.00 %2.00 %

721008 721018 C, N, O

720020 1 0.50 50255

0.60 %0.90 %2.00 %

0.30 %0.60 %2.00 %

721008 721018 C, D, O

720050 1 1.00 1005010

0.80 %1.00 %3.00 %

0.20 %0.40 %1.00 %

721007 721017 C, D, O, P,

Q

720070 1 1.00 20010020

0.60 %0.80 %2.50 %

0.20 %0.30 %0.80 %

721007 721017 C, D, P, Q

720060 1 5.00 1000500100

0.60 %0.70 %2.00 %

0.20 %0.25 %0.70 %

721006 721016 E, G, S

720110 1 50.0 500025001000

0.50 %0.60 %0.70 %

0.20 %0.30 %0.30 %

721005 721015 J

5-50 µl

0.5-10 µl

0.1-2.5 µl

1000-5000 µl

100-1000 µl

20-200 µl

10-100 µl

2-20 µl

Tip ejector allows convenient one-handed operation

Finger support keeps the instrument in place with minimum user effort

Digital display clearly reads volume setting

Ejector collar and tip cone can be removed for easy cleaning and maintenance

Durable tip cone materials provide excellent chemical resistance

All models > 10 µl offer replaceable Safe-Cone Filters to prevent internal contamination and damage

Volume setting by click stops




Comparison of the pipetting/blow-out forces needed. See page 7 for selection guide.

mLINE Proline Plus Prolinee

Tips on pages 37-46

Filters on page 36


Ergomate on page 54



Proline - a good basic pipette• Designed for many different types of routine laboratory work

• Lightweight design

• Good accuracy and precision

• Volume setting by click stops

• Full documentation, warranties and certificates

• Safe-Cone Filters to prevent internal contamination and damage

0.3/1.2 KG

0.5/1.5 KG

0.9/2.0 KG

Pipettes/


Pipettes/


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

720210 8-channel 0.10 1051

1.50 %2.50 %4.00 %

1.50 %2.50 %4.00 %

- - A, B, L, M

720220 8-channel 0.50 50255

1.00 %1.50 %3.00 %

0.50 %1.00 %2.00 %

721014 - C, D, O, P, Q

720240 8-channel 5.00 30015050

0.70 %1.00 %1.50 %

0.25 %0.50 %0.80 %

721014 - D, Q

720310 12-channel 0.10 1051

1.50 %2.50 %4.00 %

1.50 %2.50 %4.00 %

- - A, B, L, M

720320 12-channel 0.50 50255

1.00 %1.50 %3.00 %

0.50 %1.00 %2.00 %

721014 - C, D, O, P, Q

720340 12-channel 5.00 30015050

0.70 %1.00 %1.50 %

0.25 %0.50 %0.80 %

721014 - D, Q


Proline Mechanical Multichannel Performance specifications and ordering details

5-50 µl

5-50 µl

0.5-10 µl

50-300 µl

50-300 µl

0.5-10 µl

Simply turn the plunger button for volume selection

Tip ejector works with dispensing head in any position

8- and 12-channel pipettes are available

Dispensing head rotates for optimum pipetting convenience

Individual piston/tip cone assemblies allow easy repair and maintenance

Sequential tip ejection allows easy one-hand tip ejection

Tip cone design allows easy tip loading from trays and visual seal verification

Convenient pipette holder included

with each pipette

Volume setting by click stops

Tips on pages 37-46

Filters on page 36


Ergomate on page 54



e

NEW! Ergomate on page 54

All models > 10 µl offer replaceable Safe-Cone Filters to prevent internal contamination and damage

Pipettes/

Pipettes/


Pipettes

Proline carousel stand for 5 pipettes


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

722001 1-channel - 5 1.30 % 1.20 % - - A, B, C, L, M, N

722004 1-channel - 10 0.80 % 0.80 % - - A, B, C, L, M, N

722010 1-channel - 20 0.60 % 0.50 % 721008 721018 C, D, N, O

722015 1-channel - 25 0.50 % 0.30 % 721008 721018 C, D, O

722020 1-channel - 50 0.50 % 0.30 % 721008 721018 C, D, O

722025 1-channel - 100 0.50 % 0.30 % 721007 721017 C, D, O, P, Q

722030 1-channel - 200 0.40 % 0.20 % 721007 721017 C, D, P, Q

722035 1-channel - 250 0.40 % 0.20 % 721006 721016 E, G, R, S

722040 1-channel - 500 0.30 % 0.20 % 721006 721016 E, G, R, S

722045 1-channel - 1000 0.30 % 0.20 % 721006 721016 E, G, S

722050 1-channel - 2000 0.30 % 0.15 % 721005 721015 J

722055 1-channel - 5000 0.30 % 0.15 % 721005 721015 J

20 µl

5000 µl

2000 µl

1000 µl

500 µl

250 µl

200 µl

100 µl

50 µl

25 µl

10 µl

5 µl

Proline Single-Channel FIXED Volume Performance specifications and ordering details


Cat. No. Item

721000 Proline Carousel Stand for 5 mechanical pipettes

725620 Linear Stand

721259 Proline Pipette Holder for 1 pipette

721234 Colour coding caps, 6 pcs

See Proline features

on pages 19–20

Fixed volume pipette has

white display to differentiate

it from adjustable volume

Proline pipettes




Tips on pages 37-46

Filters on page 36

Ergomate on page 54



e

Pipettes/


Pipettes/

When you need to pipette and dispense liquids as comfortably as possible, your choice

should be eLINE®. Twice as fast as a mechanical pipette, this honorary mentioned (Pro

Finnish Design 2001) electronic pipette takes analytical performance and ergonomic

innovation to totally new levels! Its fully electronic operation – including tip ejection –

gives you the chance to concentrate the work-effort to where it’s needed the most. eLINE

pipettes cover the full volume range of 0.2 to 5000 µl.

Leader in performance & ergonomics – eLINE®

Autoclavable lower parts

IVD

Pipettes/

Pipettes/


Pipettes

Clear LCD-display with all the information you need

Easy-to-use keypad enables fast programming

Warning when low battery

Electronic start button for all pipetting operations

Attractive ergonomic design Volume range colour coding for

easy pipette and tip selection

Ejector collar, tip cone and piston can be unscrewed for easy cleaning, autoclaving and maintenance

Replaceable Safe-Cone Filters help prevent contamination of the pipette and samples


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

73002X 1-channel 0.05 10 51

0.2

0.90 %1.00 %2.50 %

12.00 %

0.40 %0.70 %1.50 %

10.00 %

- - A, B, L, M

73004X 1-channel 0.50 12060125

0.40 %0.60 %2.00 %3.50 %

0.15 %0.20 %1.00 %

1.50 %

721008 721018 C, D, O

73006X 1-channel 1.00 3001503010

0.40 %0.60 %1.50 %3.50 %

0.15 %0.20 %0.80 %2.00 %

721007 721017 D, Qx

73008X 1-channel 5.00 100050010050

0.40 %0.60 %1.50 %2.00 %

0.15 %0.20 %0.50 %1.00 %

721006 721016

E, G, S

73010X 1-channel 10.0 50002500500

0.50 %0.80 %1.00 %

0.15 %0.20 %0.40 %

721006 721016 J

X: 0 =Without AC-adaptor, 1 = With universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs) * Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode. The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice.

eLINE Electronic Single-Channel Performance specifications and ordering details

0.2-10 µl

5-120 µl

10-300 µl

50-1000 µl

100-5000 µl*

Both left- and right-handed operation

Direct charging option with AC-adaptor with immediate pipetting possibility

Autoclavable lower parts

Unique electronic one-touch tip ejection

Single-point calibration for specific liquids



WATCH VIDEO ONwww.biohit.com/liquid-handling

e™


Tips on pages 37-46

Filters on page 36


Ergomate on page 54




eLINE - leader in performance and ergonomics• Excellent accuracy and precision with the unique DC motor concept

• Fully electronic operations guarantee user-independent results

• Makes pipetting faster and easier

• Built-in error control for more reliable results

• Easiest tip ejection ever with the unique electronic tip ejection

• Ergonomic design that suits both left- and right-handed

• You need only one pipette instead of two – one eLINE covers the volume ranges of two mechanical pipettes

• Comprehensive range of liquid handling protocols with easy programming (see page 27)

Recommended by Health & Safety Officers

Pipettes/


Pipettes/


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)

Safe-Cone Filters Standard Plus

Tip ID

73032X 8-channel 0.05 10 5 1

0.90 %1.50 %4.00 %

0.50 %0.80 %3.00 %

- - A, B, L, M

73034X 8-channel 0.50 120 60 12

0.50 %0.70 %2.00 %

0.20 %0.30 %1.50 %

721008 721018 C, D, O

73036X 8-channel 1.00 300 150 30

0.50 %0.70 %2.00 %

0.20 %0.30 %1.00 %

721007 721017 D, Qx

73039X 8-channel 5.00 1200 600 120

0.50 %1.00 %2.50 %

0.20 %0.30 %1.00 %

721006 721016 H, T, Z

73042X 12-channel 0.05 10 5 1

0.90 %1.50 %4.00 %

0.50 %0.80 %3.00 %

- - A, B, L, M

73044X 12-channel 0.50 120 60 12

0.50 %0.70 %2.00 %

0.20 %0.30 %1.50 %

721008 721018 C, D, O

73046X 12-channel 1.00 300 150 30

0.50 %0.70 %2.00 %

0.20 %0.30 %1.00 %

721007 721017 D, Q

73049X 12-channel 5.00 1200 600 120

0.50 %1.00 %2.50 %

0.20 %0.30 %1.00 %

721006 721016 H, T, Z

eLINE Electronic Multichannel Performance specifications and ordering details

X: 0 =Without AC-adaptor, 1 = With universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs) The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice.

50-1200 µl

10-300 µl

5-120 µl

0.2-10 µl

50-1200 µl

10-300 µl

5-120 µl

0.2-10 µl

Clear LCD-display informs and instructs the user during pipetting


Volume range colour coding for easy pipette and tip selection

Dispensing head rotates to both directions without accidental disassembling for optimum pipetting convenience


Optiload tip loading mechanism

Replaceable Safe-Cone Filters help prevent contamination of the pipette and samples

Optiload tip loading and perfect tip sealing• All eLINE multichannel models are equipped with

spring loaded tip cones - the Optiload mechanism

developed by Biohit

• Allows tip loading with an equal

constant force to every channel

• Enables perfect tip sealing to every

individual tip cone

Both left- and right-handed operation

Autoclavable dispensing head (excl. 1200 µl 8/12 models)



Eject

ion

eNEW! Ergomate on page 54

Tips on pages 37-46

Filters on page 36


Ergomate on page 54




Pipettes/

Pipettes/


Pipettes

Direct charging feature allows pipetting while charging

Cat. No. Item

730981 eLINE Charging Stand for one pipette, with Universal AC-adaptor*

730991 eLINE Charging Carousel for 4 pipettes, with Universal AC-adaptor*

725620 Linear Stand

731001 eLINE Replacement Battery

The eLINE electronic pipettes can be

charged with a 1-place charging stand,

a 4-place charging carousel or a direct

charging AC-adaptor. The variety of charging

options has been designed for optimal

utility. It is possible to continue pipetting

while the eLINE is charged through the AC-

adaptor. The compact design of the 4-pipette

charging carousel is ideal for saving bench

space in the laboratory and allows the

pipette to be rested between dispensing

routines with a tip attached. The 4-place

rotating head provides easy access to the

desired unit.

eLINE charging options

• 1-Place Charging Stand

• 4-Place Charging Carousel

• AC-adaptor

Tip ejection can be laborious

and at worst a contributing

factor to Repetitive Strain

Injury (RSI). With the eLINE

this is no longer the case,

as the unique electronic tip

ejection system allows tip

removal at the light touch of

a button.

The eLINE is the only pipette

in the market that offers

electronic tip ejection. This

feature has been designed for

both left- and right-handed

operation.

The patented and unique Biohit concept includes

the DC-motor, solenoid brake and error control.

Improved accuracy and precision

The solenoid brake of the eLINE enables over 10 times more

precise positioning of the piston compared to a stepper motor

driven system. This is a major advantage in multi-dispensing

mode where liquid column has to be cut sharply to have even

doses. This means superior accuracy and precision.

Built-in error control for more reliable resultsEven the best pipette may occasionally make errors.

Important is to be able to notice them. Biohit electronic

pipettes feature a built-in error control. The pipette controls

and monitors the piston movement in real time. If an error

occurs while pipetting, the pipette notifies the user, thus

allowing for more reliable results.

Patented Biohit DC-motor concept

Unique one-touch electronic tip ejection

“eLINEistheperfectpipettetakingcareofboththeworkandthepersonusingit”

LisbethLemmetti, Development Analyst, Biomedicine

Blood Centre at Sahlgrenska University Hospital, Sweden

* incl. Euro, US/Jpn, UK and China plugs

UNIQUELYBIOHIT!

WATCH VIDEO ONwww.biohit.com/liquid-handling

Tips on pages 37-46

Filters on page 36


Ergomate on page 54




eLINE operation modes

• Pipetting (P)

• Reverse Pipetting (rP)

• Pipetting with Mixing (P+Mixing)

• Pipetting with Cycle Counter (P count)

• Manual Pipetting *1 (P manual)

• Multiple Dispensing (d)

• Diluting (dd)

• Diluting with Mixing (dd+Mixing)

• Sequential Dispensing (Sd)

• Multi-Aspirating (SA)

• Automatic Multiple Dispensing (Ad)

• 6 memory places for your

favourite pipetting protocols

• 9 speeds for both aspiration and

dispensing

For pipetting modes, please go to page 35.*1 Note: Not available in eLINE multichannel models.

Pipettes/


Pipettes/

In 1991, Biohit introduced its first electronic pipettes. Since then, Biohit Proline®

electronic pipettes have offered effortless and rapid pipetting with high levels of accuracy

and precision. Some say that the Proline range offers complete solutions for all pipetting

needs. Yes, we think that too. Proline pipettes are ergonomically designed and the tip

ejection is easily operated with four fingers to avoid strain and fatigue during pipetting.

Proline electronic is available in single- and multichannel models, covering the volume

range of 0.2 to 5000 µl.

The original electronic for all pipetting needs

Proline

IVD

Pipettes/

Pipettes/


Pipettes


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

710520 1-channel 0.10 10 5 1

0.2

0.90 %1.00 %2.50 %12.00 %

0.50 %0.80 %1.80 %

10.00 %

- - A, B, L, M

710010 1-channel 1.00 100 50105

0.40 %0.70 %2.00 %2.50 %

0.15 %0.30 %1.00 %1.80 %

- - C, O

710030 1-channel 5.00 2501252510

0.40 %0.60 %2.00 %2.00 %

0.15 %0.20 %1.00 %1.00 %

- - C, D, Q

710100 1-channel 5.00 5002505010

0.40 %0.70 %1.50 %9.00 %

0.15 %0.20 %0.80 %2.00 %

721006 721016 E, G, R

710020 1-channel 10.0 100050010050

0.60 %0.80 %2.00 %2.00 %

0.15 %0.20 %0.60 %1.00 %

721006 721016 E, G, S

710040 1-channel 5.00 120060012050

0.40 %0.70 %2.00 %2.00 %

0.15 %0.20 %0.60 %1.00 %

721006 721016 H, T, Z

710500 1-channel 50.0 50002500500

0.50 %0.80 %0.80 %

0.15 %0.25 %0.60 %

721006 721016 J

Proline Electronic Single-Channel Performance specifications and ordering details

The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice.*1 Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode.Note: Proline electronic pipette needs a charging stand for operation.

Simple keypad allows easy, error-free selection of liquid handling modes, volumes and speeds

50-1200 µl

10-250 µl

100-5000 µl

50-1000 µl

10-500 µl

0.2-10 µl

5-100 µl

Induction charging provides corrosion-free mounting

*1

Models > 250 µl offer replaceable Safe-Cone Filters to help prevent contamination and damage



Computerized drive system with fast DC-motor lets you work more efficiently than with mechanical pipettes

Compound lever design and side position gives easy tip ejection

Feather-touch button activates all pipetting actions and reduces hand fatigue

Requires charging stand



e

Tips on pages 37-46

Filters on page 36


Ergomate on page 54



Proline electronic – for all pipetting needs• Lightweight ergonomic design

• Single-point calibration for special liquids

• 5 speeds for aspiration and dispensing

• Wide volume range

• Design that helps prevent Repetitive Strain Injury (RSI)

• Option of single- or 4-place carousel charging stands (either needed for operation)

• Comprehensive range of the most important liquid handling protocols (see page 31)

Pipettes/


Pipettes/


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)

Safe-Cone filtersStandard Plus

Tip ID

710200 8-channel 0.10 1051

0.90 %1.50 %4.00 %

0.50 %0.80 %4.00 %

- - A, B, L, M

710210 8-channel 1.00 10050105

0.50 %0.70 %2.50 %4.00 %

0.20 %0.30 %1.50 %2.50 %

721014 - C, D, O, P, Q

710220 8-channel 5.00 25012525

0.40 %0.60 %1.50 %

0.15 %0.20 %1.00 %

721014 - D, Q

710800 8-channel 10.0 120060012050

0.50 %1.00 %3.00 %8.00 %

0.15 %0.20 %1.20 %1.50 %

721006 721016 H, T, Z

710300 12-channel 0.10 1051

0.90 %1.50 %4.00 %

0.50 %0.80 %4.00 %

- - A, B, L, M

710310 12-channel 1.00 10050105

0.50 %0.80 %2.50 %4.00 %

0.20 %0.40 %1.50 %2.50 %

721014 - C, D, O, P, Q

710320 12-channel 5.00 25012525

0.40 %0.60 %1.50 %

0.15 %0.20 %1.00 %

721014 - D, Q

710810 12-channel 10.0 1200 60012050

0.80 %1.00 %3.00 %8.00 %

0.15 %0.20 %1.20 %1.50 %

721006 721016 H, T, Z

Models > 10 µl have replaceable Safe-Cone Filters to help prevent contamination and damage

Proline Electronic Multichannel Performance specifications and ordering

The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice. Note: Proline electronic pipette needs a charging stand for operation.

25-250 µl

25-250 µl

5-100 µl

5-100 µl

0.2-10 µl

50-1200 µl

50-1200 µl

0.2-10 µl

Clear LCD keeps you informed of the pipette’s current task

Feather-touch button activates all piston actions

Tip ejector allows effortless tip ejection even in 12-channel models




Requires charging stand


e

Tips on pages 37-46

Filters on page 36


Ergomate on page 54



Pipettes/

Pipettes/


Pipettes

Proline charging options• 1-Place Charging Stand

• 4-Place Charging Carousel

The Proline electronic pipettes can be charged with a 1-place

charging stand or a 4-place charging carousel.

The compact design of the 4-place charging carousel is ideal

for saving bench space in the laboratory and allows the pipette

to be rested between dispensing routines. The 4-place rotating

head provides easy access to the desired unit.

Cat. No. Item

510001 Proline Charging Stand for one pipette*

511601 Proline Charging Carousel for 4 pipettes*

711002 Proline/ePET Replacement Battery

* Incl. Universal AC-adaptor (with Euro, US/Jpn, UK and China plugs)

User-independent accuracy with Biohit electronic pipettes

Whilst experience and good pipetting practice are essential for

reproducible results with mechanical pipettes, the electronic

pipettes produce precise results regardless of user experience.

Proline, like all other Biohit’s electronic pipettes, features the

patented DC-motor concept, which enables more precise and

smooth piston movement compared to mechanical pipetting.

This means superior accuracy and precision, in all pipetting

actions. Results are always reproducible even with small vol-

umes independent on experience, as hand control is totally

eliminated.

Nine persons were divided into three groups according to their experi-ence in pipetting. Each person pipetted distilled water with the same Biohit Proline mechanical and electronic pipettes (using P-mode). The imprecision of ten subsequent results of volumes 10, 25, 50, 100, 200 and 1000 µl was recorded and the results are detailed in pictures on the right.

Volume (µl)

Volume (µl)

Volume (µl)

Impr

ecis

ion

(µl)

Impr

ecis

ion

(µl)

Impr

ecis

ion

(µl) 4

3

2

1

01000 200 100 50 25 10

1000 200 100 50 25 10

1000 200 100 50 25 10

Mechanical Electronic

The Effect of User Experience on Pipetting Precision

Expe

rts

Mod

erat

eEx

peri

ence

No

Expe

rien

ce

4

3

2

1

0

4

3

2

1

0

Experts

Moderate experience

No experience

Stands & AccessoriesTips on pages 37-46

Filters on page 36


Ergomate on page 54



Proline operation modes• Pipetting (P)

• Pipetting with mixing (P+Mixing)

• Reverse Pipetting (excl. 1200 and 5000 models) (rP)

• Multiple Dispensing (d)

• Diluting (excl. multichannel 1200 model) (dd)

• Diluting with mixing (excl. multichannel 1200 model)

(dd+Mixing)

• Sequential Dispensing (only 1200 and 5000 models) (Sd)

• Multi-Aspirating (only 1200 models) (SA)

For pipetting modes, please go to page 35.

Pipettes/


Pipettes/

Biohit ePET® electronic pipettes offer an easy and affordable access to electronic

pipetting. By using a direct charging system which requires no charging stand, ePET is a

very cost-effective choice for electronic pipetting! The ePET is available in single-channel

models covering the volume range of 0.2 to 5000 µl and multichannel models from 0.2 to

1200 µl.

The compact and economical alternative – ePET®

IVD

Pipettes/

Pipettes/


Pipettes

The compact and economical alternative – ePET®


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)


Tip ID

710521ET 1-channel 0.10 10 51

0.2

0.90 %1.00 %2.50 %

12.00 %

0.50 %0.80 %1.80 %

10.00 %

- - A, B, L, M

710011ET 1-channel 1.00 10050105

0.40 %0.70 %2.00 %2.50 %

0.15 %0.30 %1.00 %1.80 %

- - C, O

710021ET 1-channel 5.00 100050010050

0.40 %0.70 %1.50 %2.00 %

0.15 %0.20 %0.50 %1.00 %

721006 721016 E, G, S

710041ET 1-channel 5.00 120060012050

0.40 %0.70 %2.00 %2.00 %

0.15 %0.20 %0.60 %1.00 %

721006 721016 H, T, Z

710501ET 1-channel 50.0 50002500500

0.50 %0.80 %0.80 %

0.15 %0.20 %0.30 %

721006 721016 J

ePET Electronic Single-Channel Performance specifications and ordering details

50-1000 µl

0.2-10 µl

5-100 µl

100-5000 µl

50-1200 µl

IncludesUniversalAC-adaptor(incl.Euro,US/Jpn,UKandChinaplugs)The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice. *1 Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode.

Direct charging makes the ePET a cost-

effective alternative for electronic pipetting

Cat. No. Item

710999 ePET Stand for one pipette

725620 Linear Stand

711002 Proline/ePET Replacement Battery

*1

Direct charging using AC-adaptorClear LCD display keeps you informed of the pipette’s current task Simple keypad allows easy, error free

selection of liquid handling modes, volumes and speeds

Feather-touch button activates all piston actions

Compound lever design and side position provides easy tipejection Volume range colour coding



Tip cone design allows easy tip mounting from trays and visual seal verification

Models >100 µl offer replaceable Safe-Cone Filters to help prevent contamination and damage

Requires no charging stand




e

Tips on pages 37-46

Filters on page 36

Ergomate on page 54



ePET – compact and economical• Pipetting (P)

• Pipetting with Mixing (P+Mixing)

• Multi Dispensing (d)

• Diluting (dd)

• Diluting with Mixing (dd+Mixing)

• 5 speeds for aspirating and dispensing

Pipettes/


Pipettes/

ePET® Electronic Multichannel Pipettes

ePET multichannel pipette family


Increment(µl)

Test Volume (µl)

Inacc.(%)

Impr.(%)

Safe-Cone filtersStandard Plus

Tip ID

710201ET 8-channel 0.10 1051

0.90 %1.50 %4.00 %

0.50 %0.80 %4.00 %

- - A, B, L, M

710211ET 8-channel 1.00 10050105

0.50 %0.70 %2.50 %4.00 %

0.20 %0.30 %1.50 %2.50 %

721014 - C, D, O, P, Q

710221ET 8-channel 5.00 25012525

0.40 %0.60 %1.50 %

0.15 %0.20 %1.00 %

721014 - D, Q

710801ET 8-channel 10.0 120060012050

0.50 %1.00 %3.00 %8.00 %

0.15 %0.20 %1,20 %1.50 %

721006 721016 H, T, Z

710301ET 12-channel 0.10 1051

0.90 %1.50 %4.00 %

0.50 %0.80 %4.00 %

- - A, B, L, M

710311ET 12-channel 1.00 10050105

0.50 %0.80 %2.50 %4.00 %

0.20 %0.40 %1.50 %2.50 %

721014 - C, D, O, P, Q

710321ET 12-channel 5.00 25012525

0.40 %0.60 %1.50 %

0.15 %0.20 %1.00 %

721014 - D, Q

710811ET 12-channel 10.0 120060012050

0.80 %1.00 %3.00 %8.00 %

0.15 %0.20 %1.20 %1.50 %

721006 721016 H, T, Z

ePET Electronic Multichannel Performance specifications and ordering details

IncudesUniversalAC-adaptor(incl.Euro,US/Jpn,UKandChinaplugs)The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit’s continuous R&D, specifications may change without prior notice.

50-1200 µl

0.2-10 µl

50-1200 µl

5-100 µl

25-250 µl

0.2-10 µl

25-250 µl

5-100 µl

Direct charging feature allows pipetting while charging

Clear LCD-display keeps you informed of the pipette’s current task

Tip ejector allows effortless tip ejection even on 12-channel models

Volume range colour coding




Safe-Cone Filters for models > 10 µl


Requires no charging stand

Tips on pages 37-46

Filters on page 36

Ergomate on page 54



e

Pipettes/

Pipettes/


Pipettes

Tips on pages 37-46

Filters on page 36

Ergomate on page 54



Pipetting modes for Biohit electronic pipettes

Pipetting (P) Sequential Dispensing (Sd)

Program up to 12 aliquot volumes per aspirationA series of different volumes can be delivered in any desired

order. An extremely beneficial feature when performing

dilution series.

Automatic blow-outPipette a selected volume with automatic blow-out with

complete control of the piston movement. Post-delivery mixing

may be used in conjunction with this mode.

Reverse Pipetting (rP) Automatic Dispensing (Ad)

Efficient automatic dispensing with user-defined pace control This mode is similar to the multiple dispensing mode but has

the additional benefit of the dispensing cycles being under

automatic control at a user specified pace (0.1 – 9.9 seconds).

Optimises performance when pipetting viscous or biological fluidsA selected volume plus an excess is aspirated into the tip.

After delivery the excess volume remains in the tip and is

discarded.

Multiple Dispensing (d) Multi-Aspirating (SA)

Manual method for washing microplate stripsSelect the volume and the number of aspirations until the series

is completed, then discard the full aspirated volume in a single

step.

Quickly dispenses equal volumesAspirate the total volume plus an excess and repetitively

dispense equal volumes of liquid. The volume and number

of remaining aliquots are shown on the display.

Diluting (dd) Post Delivery Mixing (* or + Mixing)

User controlled mixing timeThe piston is automatically moved up and down to mix the

liquid in the delivery vessel. The mixing time is controlled by

the start button. The mixing function is available in the pipetting

or diluting modes (P, dd).

Dilution ratios 1:2 to 1:50Two different solutions separated by an air gap are aspirated and

then dispensed together with automatic blow-out. This mode

may be used also in conjunction with post-delivery mixing.

To see which pipette models offer these features, go to Selection Guide on page 7

Pipettes/


Pipettes/

Cat. No. Description Qty/Unit

721008 Standardø 2.51 mm

PE

50 Filters


PE

50 Filters


PE

50 Filters


PE

50 Filters


PE

50 Filters

721018 Plusø 2.51 mm

PE

50 Filters

721017 Plusø 3.15 mm

PE

50 Filters

721016 Plusø 5.33 mm

PE

50 Filters

721015 Plusø 6.73 mm

PE

50 Filters

Safe-Cone Filters Ordering details

Pipette contamination in pipette barrel when pipetting liquid culture of bacteria Micrococcus Luteus.

The interval of changing the filter depends completely on

the application and the sample. However, according to

studies the filter is recommended to be changed daily (af-

ter 50 to 250 pipettings) and immediately in case of over-

aspi ration.

No. of Pipettings

No Filter

Safe-Cone Filter

50 - -

100 + -

250 ++ -

500 +++ -

+: DNA (50 µl plasmid DNA 120 µg/ml) contamination in pipette barrel.

Number of pipettings

cfu

10x 100x 250x 500x

1000

0

2000

3000

4000

5000

6000

7000

8000

Filter

No filter

PE=polyethylene

Safe-Cone Filters

Why should you use Safe-Cone Filters?These unique and replaceable polyethylene (PE) filters act as

a final barrier to prevent any fluids and liquid vapours from

reaching the internal components of the pipette.

• Protect the pipette and sample from contamination

• Prolong the pipette’s lifetime

• Reduce maintenance intervals

• Cost-effective

UNIQUELYBIOHIT!

Change daily!

How often should you change?

How to change?

When should you use them?

Standard FilterFor general applications. Can be used in same type of work as the Plus filter is recommended for, but needs to be changed more frequently.

Plus Filter For more demanding applications such as radioactive work, cell culture, bacterial and virological work and molecular biology.

The ultimate pipette protectors are available in two types:

For more info, see literature on www.biohit.com and page 94.

To ensure the safety of the user, forceps should be used

to avoid touching the contaminated filters by hand or use

unique built-in filter ejector in mLINE. In addition, clean

the tip cone with ethanol (70%) prior to the assembly of

a new filter.

Built-in filter ejector in mLINE

Forceps included in each pipette package (excl. mLINE)

Pipettes/

Pipettes/


Dispensers & Tips

Optifit Pipette Tips

Pipettes/


Pipettes/

Biohit offers you precision-made pipette tips for use with all Biohit and most other

pipette makes providing excellent reproducibility and accuracy. You will find these virgin

polypropylene tips in single trays as non-sterile or pre-sterilized, in refills and in bulk

packaging. And last but not the least, Biohit tips are manufactured in a protected, ISO 8

Clean Room environment, following Good Manufacturing Practices (GMP), causing as little

environmental loading as possible.

Fully autoclavable

IVD

Environmentally friendly material and manufacturing

Biohit Optifit Tips - the perfect match for your pipette

Pipettes/

Pipettes/



Biohit Optifit Tips

Providing for all needs of pipetting• Both non-filtered and filtered tips available

• SafetySpace™ Filter Tips - whenever extra space is needed

between the sample and the filter

• Innovative and ecological packaging choices

• All non-filtered tips, trays and racks are autoclavable

Best fit - best possible accuracy• Perfect fitting and sealing with Biohit pipettes secures the

best possible accuracy and precision

• Ergonomic and light tip attachment and ejection with

Optiload in Biohit eLINE , mLINE and Proline Plus pipettes

• Colour coding of the tip trays allows for easy matching

with a suitable colour coded Biohit pipette

• Compatible with most other pipette makes (see

compatibility with other manufacturers’ pipettes at

www.biohit.com)

In liquid handling, the pipette and the tip always form a system.

Biohit tips have been developed to perfectly match Biohit pipettes.

By developing and producing both pipettes and tips Biohit is able

to offer the best possible compatibility and pipetting results.

For example, the Optiload tip loading mechanism developed by

Biohit in mLINE, Proline Plus and eLINE pipettes allows tips to

be loaded with constant force, which, in turn, secures optimal tip

sealing and minimum tip ejection force.

As the tip is an integral component of the pipetting system, its

shape, material properties and fit have a considerable influence

on pipetting accuracy. Variation in any of these will cause errors

in the volume pipetted.


Eject

ion

Optiload tip loading mechanism for perfect tip sealing and light tip ejection

Packaging options on page 41

Biohit Optifit Tips - the perfect match for your pipette

Premium quality and purity• Strict quality standards, ISO 9001 and ISO 13485,

followed from R&D to production and delivery

• CE/IVD certified

• Manufactured in ISO 8 classified clean room conditions

• The highest quality virgin polypropylene used as raw

material

• DNase, RNase and endotoxin free manufacturing process

- Single Trays and Refill Packs certified safe by lot number

• Pre-sterilized tips are e-beam irradiated

• All tips, including individual racks, are lot numbered for

full traceability

Pipettes/


Pipettes/

Especially useful in the following applications which require extra volume in the tip:• pipetting foaming liquids like buffers and proteins• pipetting solvents• multiple dispensing functions of electronic pipettes• reverse pipetting

Ideal tool in• molecular biology• microbiology• cell culture applications• radioactive work

The tips are packed in colour-coded single tray racks. They are

certified DNase, RNase and endotoxin free and are e-beam

pre-sterilized.

SafetySpace Filter Tips have more space between the sample and

the filter than conventional filter tips. With SafetySpace Filter Tips

you do not need to worry about the sample touching the filter

whatever pipetting technique or type of liquid you’re using.

SafetySpace™ Filter Tips for safer pipetting and better accuracyBiohit’s SafetySpace Filter Tips are made of virgin polypropylene

and feature filter (PE) barriers to prevent aerosol and liquid con-

tamination. This helps protect against the risk of cross-contami-

nation and reduces maintenance requirements of the pipette.

Optifit Tips, non-filteredBiohit’s non-filtered Tips are made of premium-

grade virgin polypropylene. The tips are metal-

free and available in autoclavable (121°C; 20 min; 1 bar) Single

Tray racks (as certified RNase, DNase and endotoxin free), in

space saving Eco Refill System and in Bulk packages. Electron

beam pre-sterilized tips in racks and Refill Packs are also avail-

able.

Non-Filtered & Filtered Tips

Pipettes/

Pipettes/



Packaging options

Single Refill Packs - ecological and contamination free• Materialusedintips,traysandcontainersarerecyclable- container lid is regular waste

• CompatiblewithSingleTrayracks

• Individually packed Single Tray racks

• DNase, RNase and endotoxin free certified by lot number

• Also available as e-beam pre-sterilized

• Covers most tip sizes: 10 µl, 300 µl, 350 µl, 1000 µl 1200 µl and 1200 µl extended

Refill Towers - ecological and space-saving• Completelyrecyclable

• CompatiblewithSingleTrayracks

• Covers the most popular, non-filtered tips sizes: 10 µl, 300 µl and 350 µl

Eco Refill System

Single Tray racks - for safer laboratory work• Materialusedintips,traysandracks(polypropylene)is100%recyclable

• Fully autoclavable (121º, 20 min) rackscanbereusedwithRefillTips

• Air-tight plastic wrapping for maximal purity - wrapping is regular waste

• Flip-top boxes can be easily opened and closed using a single hand

• Colour coding of the tip trays allows for easy matching with a suitable Biohit pipette

• RNase, DNase and endotoxin free certified by lot Number.

• Also available as e-beam pre-sterilized

• Filter Tips available as pre-sterilized only (non-autoclavable)

• New rack labels indicate tip volume, lot number, etc.

Bulk package - economic option• Completelyrecyclable

• Available for non-filtered tips in boxes with 100, 400 or 1000 tips

• Packed in plastic bag inside the cardboard box

• Manufactured according to the same quality requirements as all other Biohit Tips

Your ecological

choice!

Environmental facts:Materials are recyclable • Plastics can be incinerated as energy waste • Cardboard packaging is renewable

Eco Refill System is space saving and produces less waste

Environmentally friendly, modern manufacturing technology is used • Effective use of raw material • Less waste generated

Up to 61% less waste by using Refill Tips instead of racked tips

Non-Filtered & Filtered Tips

Pipettes/


Pipettes/

Optifit Tips, non-filtered

Wide Bore Tip

Extended

Extended

Tip Specifications

Tip Tip ID Volume Range Length Packaging Purity Level Qty/Unit Cat. No.

RNase, DNase, endotoxin free

Pre-Sterilized

A 0.1 - 10 µl 31.5 mm Single TraySingle TrayRefill Tower Refill PackBulk in Box

••

•

•

•

10 x 9610 x 9610 x 96 20 x 961000

790010790011790012790013781349

B 0.1 - 10 46 mm Single Tray Single Tray

•• •

10 x 96 10 x 96

783210 783211

C 0.5 - 300 µl 51 mm Single TraySingle TrayRefill TowerRefill PackBulk in Box

•• •

• •

10 x 9610 x 9610 x 9615 x 96 1000

790300790301790302 790303780011

D 5 - 350 µl 54 mm Single TraySingle TrayRefill TowerRefill PackBulk in Box

••

•

• •

10 x 9610 x 9610 x 9615 x 961000

790350790351790352790353780033

E 10 - 1000 µl 71.5 mm Single TraySingle TrayRefill PackRefill PackBulk in BoxBulk in Box

••••

• •

10 x 9610 x 9610 x 9610 x 96

4001000

791000791001791002791003780021780016

G 10 - 1000 µl 68.5 mm Single TraySingle TrayBulk in Box

••

•

10 x 9610 x 961000

791020791021780053

H

Z

50 - 1200 µl

50 - 1200 µl

71.5 mm

90 mm

Single TraySingle TrayRefill PackRefill PackBulk in Box

Single TraySingle TrayRefill PackRefill Pack

•••• ••••

• • • •

10 x 9610 x 9610 x 9610 x 961000

10 x 9610 x 9610 x 96 10 x 96

791200791201791202791203780043

791210791211791212 791213

J 100 - 5000 µl 150 mm Single TraySingle TrayBulk in BoxBulk in Carton

••

•

50501001000

780304780305780300780308

K 0.5 - 10 ml 242 mm Bulk in Box 100 780310

U 1.10 ml 161 mm Bulk in box 250 780315

Extra long Tip for XL and MidiPlus

Pipettes/

Pipettes/



SafetySpace™ Filter Tips

Filter Tips with regular air gap

For your guidance the tips are illustrated at actual size.


RNAse, DNase, endotoxin free

Pre-Sterilized

L 0.1 - 10 µl 32 mm Single Tray • • 10 x 96 790011F

N 0.5 - 20 µl 51 mm Single Tray • • 10 x 96 790021F

O 2 - 120 µl 51 mm Single Tray • • 10 x 96 790101F

P 5 - 200 µl 54 mm Single Tray • • 10 x 96 790201F

Q 5 - 300 µl 52.5 mm Single Tray • • 10 x 96 790301F

S 50 - 1000 µl 78 mm Single Tray • • 10 x 96 791001F

T 50 - 1200 µl 90 mm Single Tray • • 10 x 96 791211F


RNAse, DNase, endotoxin free

Pre-Sterilized

M 0.1 - 10 µl 46 mm Single Tray • • 10 x 96 783201

R 10 - 500 µl 78 mm Single Tray • • 10 x 100 783206

Empty Tip Boxes for Refill System Item Tip ID Tip Type (Standard Tips) Qty/Unit Cat. No.

Empty Tip Box for Refill System A, C, D 10, 300, 350 µl 10 790910

Empty Tip Box for Refill System E, H 1000, 1200 µl 10 790920

(tips and trays are not included)

RNase, DNase & Endotoxin Certificates for Single Tray Tips can be downloaded using the lot number* from www.biohit.com/tipquality* available for lots manufactured after June 2005

Pipettes/


10 10 Ext

300

350 10

0012

0050

0010

000 E

xt.


10 10 Ext.

**

20 120

200

300

500 *

*10

0012

0010

00 W

B


1200

Ext.

Electronic Pipettes

Compatibility charts

12-Channel

8-Channel

Cat. No. µl A* B C D E G H Z J K L* M N O P Q R S T

73002X 0.2-10 • • • •

73004X 5-120 • • •

73006X 10-300 • •

73008X 50-1000 • • •

73010X 100-5000 •

73032X 0.2-10 • • • •

73034X 5-120 • • •

73036X 10-300 • •

73039X 50-1200 • • •

73042X 0.2-10 • • • •

73044X 5-120 • • •

73046X 10-300 • •

73049X 50-1200 • • •

710520 0.2-10 • • • •

710010 5-100 • •

710030 10-250 • • •

710100 10-500 • • •

710020 50-1000 • • •

710040 50-1200 • • •

710500 100-5000 •

710200 0.2-10 • • • •

710210 5-100 • • • • •

710220 25-250 • •

710800 50-1200 • • •

710300 0.2-10 • • • •

710310 5-100 • • • • •

710320 25-250 • •

710810 50-1200 • • •

71052XET 0.2-10 • • • •

71001XET 5-100 • •

71002XET 50-1000 • • •

71004XET 50-1200 • • •

71050XET 100-5000 •

71020XET 0.2-10 • • • •

71021XET 5-100 • • • • •

71022XET 25-250 • •

71080XET 50-1200 • • •

71030XET 0.2-10 • • • •

71031XET 5-100 • • • • •

71032XET 25-250 • •

71081XET 50-1200 • • •

71090X 0.1-25 ml • •

71093X 1-100 ml • •

eLINE

Single Channel

12-Channel

8-Channel

Proline Single Channel

8-Channel

12-Channel

ePET

Single Channel

Proline XL

Midi Plus

* Extended inaccuracy and imprecision of the pipettes with pre-sterilized A tips.** Filter Tips with regular air gap

See tip compatibility with other manufacturers’ pipettes at www.biohit.com

Pipettes/

10 300

350

1000

1000

WB

5000

10 00

0


10 10 Ext.

**

20 120

200

300

500 *

*10

0010

Ext


Mechanical Pipettes

12-Channel

8-Channel

Cat. No. µl A* B C D E G J U L M N O P Q R S

725010 0.1-3 • • • •

725020 0.5-10 • • • •

725030 2-20 • •

725050 10-100 • • •

725060 20-200 • • • •

725070 100-1000 • • •

725080 500-5000 •

725090 1-10 ml •

725120 0.5-10 • • • •

725130 5-100 • • •

725140 30-300 • •

725220 0.5-10 • • • •

725230 5-100 • • •

725240 30-300 • •

728010 0.1-3 • • • •

728020 0.5-10 • • • •

728030 2-20 • •

728050 10-100 • • •

728060 20-200 • • • •

728070 100-1000 • • •

728080 500-5000 •

728090 1-10 ml •

728590 10 ml •

728120 0.5-10 • • • •

728130 10-100 • • •

728140 30-300 • •

728220 0.5-10 • • • •

728230 10-100 • • •

728240 30-300 • •

728515 5 • • • •

728520 10 • • • •

728530 20 • •

728535 25 • •

728545 50 • •

728550 100 • • •

728560 200 • • • •

728565 250 • • • •

728567 500 • • • •

728570 1000 • • •

728575 2000 •

728580 5000 •

728590 10 ml •

720005 0.1-2.5 • • • •

720000 0.5-10 • • • • • •

720080 2-20 • • •

720020 5-50 • • •

720050 10-100 • • • • •

720070 20-200 • • • •

720060 100-1000 • • •

720110 1000-5000 •

720210 0.5-10 • • • •

720220 5-50 • • • • •

720240 50-300 • •

720310 0.5-10 • • • •

720320 5-50 • • • • •

720340 50-300 • •

722001 5 • • • • • •

722004 10 • • • • • •

722010 20 • • • •

722015 25 • • •

722020 50 • • •

722025 100 • • • • •

722030 200 • • • •

722035 250 • • • •

722040 500 • • • •

722045 1000 • • •

722050 2000 •

722055 5000 •

mLINE

Single Channel

Proline Fixed VolumeSingle Channel

8-Channel

12-Channel

Proline Adjustable VolumeSingle Channel

* Extended inaccuracy and imprecision of the pipettes with pre-sterilized A tips.** Filter Tips with regular air gap

Proline Plus

Adjustable VolumeSingle Channel

8-Channel

12-Channel

Proline Plus

Fixed VolumeSingle Channel

Pipettes/


Pipettes/

Premium tip qualityISO 8 Clean Room manufacturing - contamination free tipsTo avoid contamination from human contact, we have automated the whole manufacturing process. Pure virgin polypropylene (PP) plastic is automatically fed from silos to moulding machines. The moulding machines and robots that are located in isolated clean cells load the tips automatically to the tip trays and packaging. HEPA filters and higher air pressure are applied for purity within the cell. All Biohit tip racks and Single Refill Packs are individually and automatically packed in air-tight plastic to rule out any danger of contamination.

Additionally, our highly experienced and trained personnel is equipped with specially designed clothing, masks, hair nets and gloves to further diminish the contamination risks.

An independent laboratory checks each Single Tray and Refill Pack tip lot for RNase, DNase and Endotoxins. The Certificate of Quality can be downloaded at www.biohit.com/tipquality

As the tip is an integral component of the pipetting sys-tem, its shape, material properties and fit have a consider-able influence on pipetting accuracy.

To ensure high quality and accurate pipetting we check each tip lot for• Geometry and shape of the tip• Occurence of scratches, mechanical

fails, air bubbles, plastic depris and impurities

• Sealing to tip cone• Functionality together with a pipette for

straight fluid flow• Cleanliness of the tip

All Biohit tips are fully traceable. Mould cavity identification markings are printed on each tip, and tip racks and packages are lot numbered.

Our quality management system follows not only ISO 9001 and ISO 14001, but also ISO 13485 (ISO standard for medical devices), which enables us to give CE / IVD (In Vitro Diagnostics) certification for both pipettes and pipette tips. The tip production also follows ISO 14644-1 standard to fulfill ISO Class 8 cleanroom conditions.

Traceability

Pipettes/

Pipettes/


Dispensers & Tips

Dispensers & Tips

Pipettes/


Pipettes/

A single innovation for repetitive dispensingseLINE Pro and Lite Dispensers hand you the most convenient and ergonomic approach to

multiple dispensing. The automated liquid handling operation offers accurate and repetitive

dispensing also with viscous, infectuous and high vapour pressure liquids. The unique TipGuide

selects and displays the most suitable tip size, which saves time and eliminates the possibility of

an incorrect tip selection. Additionally, a smart feature included in the whole eLINE product range

is the unique electronic tip ejection, which reduces the risk of RSI (Repetitive Strain Injury).

IVD

For

the

Best

Tip Choice

Tip G uid e

Pipettes/

Pipettes/


Dispensers & Tips

eLINE® Lite & Pro Dispensers

Both Dispensers offer• TipGuide – automatic guide for correct tip selection

• Outstanding ergonomics – dispensing with minimum force

• Unique electronic feather light tip ejection

• Contamination-free dispensing with positive displacement

principle

• Volume range from 1 µl to 50 ml

• 5 speeds for aspiration and dispensing

• Direct charging option enabling dispensing while charging

• Compatibility with eLINE charging stand and carousel

• Compatibility with Biohit Dispenser tips and Eppendorf

CombitipsPlus tips.

Operating modes eLINE Pro eLINE Lite

Multiple Dispensing with the Tip Guide (d) • •

Multiple Dispensing (d) •

Pipetting (P) •

Diluting (dd) •

Sequential Dispensing (Sd) •

Automatic Multiple Dispensing (Ad) •

Multi-Aspirating (SA) •

Custom mode (CST) - GL (select service intervals) - Sr (select the desired tip range) - SET (returns default settings)

•

5 speeds for aspirating and dispensing • •

eLINE Lite & Pro Dispenser Operating Modes

New member of the eLINE family!

eLINE Lite and eLINE Pro – what is the difference?

eLINE Lite Dispenser – for ease of use in multiple dispensingseLINE Lite is designed for Multiple Dispensing (d) mode

with the Tip Guide, and it is quick and simple to operate.

eLINE Pro Dispenser – for more versatile useeLINE Pro offers, in addition to the basic Multiple Dispensing

(d) mode, a comprehensive range of liquid handling protocols

with easy programming (see table below for all available

modes).

Pipettes/


Pipettes/

Direct charging option with AC-adaptor with immediate dispensing possibility

Clear LCD-display with charging indicator


Electronic start button for all pipetting operations

Attractive ergonomicdesign

Easy & light tip insertion

Both left- and right-handed

operation


Cat. No. Item

73070X Biohit eLINE Pro Dispenser

73080X Biohit eLINE Lite Dispenser

73098X Biohit eLINE Charging Stand, with AC-adaptor

73099X eLINE Charging Carousel for 4 pipettes, with AC-adaptor

731001 eLINE replacement battery

eLINE Dispenser Ordering details

X: 0 =Without AC-adaptor, 1 = With Universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs)Biohit has several worldwide patents and patents pending for its products.

Save your time, let the TipGuide make the tip choice for you• No time consuming volume calculations

• Just set the desired volume and number of dispens ings,

the TipGuide selects and displays the suitable tip size

For

the

Best

Tip Choice

Tip G uid e

Pipettes/

Pipettes/


Dispensers & Tips

Biohit Mechanical Stepper

The Biohit Mechanical Stepper is an easy to use positive

displacement dispenser which allows rapid multi-dispensing of

pre-set volumes up to 48 times in succession without refilling.

The unit comes complete with an adapter for 25 ml and 50 ml

tips.

Features include:

• Easy single handed operation

• New, more ergonomic design with improved

finger support

• Lightweight (105 g) yet robust construction

• Maintenance-free design

• Compatible with a wide range of dispenser tips

• Ideal for dispensing aqueous and viscous liquids

• Dispensing volumes from 1 µl to 5000 µl

(min. volume with Biohit Dispenser Tips is 2 µl)

• Its ergonomic design and positioning of the dosage

button in the upper part of the device allow single-handed

volume selection, loading and dispensing.

• 48 dispensing steps at intervals of 1 second without the

need to refill thus saving 90 % of the working time

required by the usual pipetting technique

• Tested according to ISO 8655-1

Cat. No. Description

725700 Biohit Mechanical Stepper including adapter for 25 ml and 50 ml syringe tips

Ordering Details

Tip volume (ml) Dosage volume Imprecision (%) Inaccuracy (%)

Adjustment on dispenser

1 2 3 4 5

Number of steps 48 23 15 11 8

0.10 2 µl 4 µl 6 µl 8 µl 10 µl < 1.6 % < ± 3.0 %

0.20 4 µl 8 µl 12 µl 16 µl 20 µl < 1.3 % < ± 2.0 %

0.50 10 µl 20 µl 30 µl 40 µl 50 µl < 0.5 % < ± 0.8 %

1.00 20 µl 40 µl 60 µl 80 µl 100 µl < 0.9 % < ± 0.9 %

2.50 50 µl 100 µl 150 µl 200 µl 250 µl < 0.4 % < ± 0.8 %

5.00 100 µl 200 µl 300 µl 400 µl 500 µl < 0.3 % < ± 0.4 %

10.00 200 µl 400 µl 600 µl 800 µl 1000 µl < 0.5 % < ± 0.6 %

25.00 500 µl 1000 µl 1500 µl 2000 µl 2500 µl < 0.3 % < ± 0.2 %

50.00 1000 µl 2000 µl 3000 µl 4000 µl 5000 µl < 0.2 % < ± 0.2 %

Biohit Mechanical Stepper and Biohit Dispenser Tip system Performance specifications

The data for tips 0.5 and 2.5 ml are based on a measurement carried out with a 100 µl pipette tip.The performance specifications apply to the Biohit Mechanical Stepper and Biohit Dispenser Tips (non-sterile) System.

More ergonomic finger support

Pipettes/


Pipettes/Biohit Dispenser Tips

Tip volume (ml)

Volume min/max (µl)

Increment (µl)

Step size max (µl)

Test volume (µl)

Inacc. (%)

Impr. (%)

Number of dispensings min/max

0.1 1/100 0.2 100 10 100

1.00 %1.00 %

2.00 %0.50 %

1/100

0.2 2/200 0.4 200 20 200

1.70 %1.00 %

2.50 %0.50 %

1/100

0.5 5/500 1 500 50 500

1.00 %0.80 %

3.00 %0.60 %

1/100

1.0 10/1000 2 1000 100 1000

0.50 %0.50 %

0.60 %0.20 %

1/100

2.5 25/2500 5 2500 250 2500

0.60 %0.60 %

0.40 %0.20 %

1/100

5.0 50/5000 10 5000 500 5000

0.60 %0.60 %

0.50 %0.15 %

1/100

10.0 100/10 000 20 10 000 1000 10000

0.40 %0.40 %

1.00 %0.20 %

1/100

25.0 500/25 000 50 25 000 2500 25000

0.50 %0.50 %

0.50 %0.15 %

1/50

50.0 1000/50 000 100 50 000 5000 50000

0.30 %0.30 %

0.70 %0.15 %

1/50

eLINE Pro and Lite Dispenser and Biohit Dispenser Tip system Performance specifications

The performance specifications apply to the eLINE Dispenser and Biohit Dispenser Tip System.Due to Biohit’s continuous R&D, specifications may change without prior notice.

Volume (ml) Package Qty/Unit Cat. No.

Starter Kit Includes 20 * 0.5 ml 20 * 1.0 ml 20 * 2.5 ml 20 * 5.0 ml 20 * 10.0 ml

100 792038

0.1 Non sterilePre-sterilized

100100

792017792026


100100

792018792027


100100

792019792028


100100

792020792029


100100

792021792030


100100

792022792031


100100

792023792032

25.0 Non sterile Pre-sterilized

25 25

792024 792033

50.0 Non sterile Pre-sterilized

25 25

792025 792034

Adapter for 25 and 50 ml tips, 3 pcs./unit 792036

Biohit Dispenser Tip package Ordering details

* Includes 1 adapter/unit

*

* *

*

IVD

Features:• Function according to positive displacement principle

• Contamination free dispensing – no aerosols are formed

• Made of virgin polypropylene (tip) and polyethylene (plunger)

• 9 different tip sizes from 0.1 to 50 ml

• DNA, RNase, ATP and Endotoxin free

• Pre-sterilized tips are individually wrapped

• Suitable for use together with eLINE Pro and Lite Dispensers

and Biohit Mechanical Stepper, as well as most other

mechanical steppers (Ripette (Ritter), HandyStep (Brand),

Minilab 100/101 (HTL), EasyStep)

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Dispensers & Tips

Accessories

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Ergomate – ergonomic pipetting system

Cat. No. Item

723100 Ergomate Starter Kit, includes 3 Ergomate Plate holders and grids

723101 Ergomate Micro Plate Grids double-sided pack of 10

Ergonomic angle for proper po-sitioning of wrist and hand for more comfortable pipetting.

Micro Plate Grids

Plate holder can be reversed and used for flat surface pipetting or as an incubation station with lid

Ideal for safe transporting of plates

When reversed plate holder creates an ideal aerosol barrier for use in hoods or on bench top surfaces

Microplate grids for quick identification and sample management

Double-sided. Reverse side featuring numbering from 1-12.

UNIQUELYBIOHIT!

When pipetting, ISO 8655 recommends touching off samples

on the wall of receiving vessels, especially with small volumes.

The new Biohit Ergomate removes the need to hold the plate at

any specific angle and allows for safe dispensing, resulting in

easier, safer, and more precise pipetting.

™


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Accessories

Cat. No. Qty/Unit Capacity

783500 16 120 ml

The autoclavable and durable reagent vessel is made from polypropylene and is chemically resistant to all common reagents.

Reagent Vessel

Biohit Proline® Biocontrol – decontamination solution

Biohit Proline Biocontrol is a safe, simple solution for de con ta mi -

na tion in the la bo ra tory. It is spe ci fi cally de signed for de con ta mi -

na ting la bo ra to ry instru ments.

The components of the pipette, which most often become con-

taminated, whether by incorrect pipetting tech niques or by

accident, are in the tip cone area.

Daily safety maintenanceLightly spray the outside of the pipette and wipe dry with a

clean cloth. Clean surfaces, such as incubators and benches

with no noticeable contamination such as blood stains may be

dampened using the Proline Biocontrol, left on for 2 minutes

and then wiped clean with an absorbent cloth.

Effective against:

•VirusesHepatitis A, B, C, D and E, HIV, Parvovirus, Newcastle Disease

•BacteriaPseudomonas sp., coli, Staph aureus, Proteus mirabilis,

Proteus vulgaris, Streptococcus sp., Bacillus sp., Salmonellae,

Klebsiella sp., Enterobacter sp., Serratia sp., Mycobacterium

sp., Listeria sp., Legionella pneumophilia

•FungiA. Niger, Penicillium sp., T. Mentagrophytes, Candida albicans

Cat. No. Qty/Unit Pack Format

724004 5 litre Economic Large Container

Biohit Proline® Biocontrol is:• Non-toxic and non-irritating to humans and animals

• Ready to use

• Lethal to a broad spectrum of viruses, bacteria and fungi

• BS 6471 Approved

• Non-corrosive to metals

• Non-fuming

• Odourless and non-staining

• Non-mutagenic

• Alcohol, aldehyde, phenol and chlorine free

• Effective at removing odours

• Environmentally friendly (biodegradable)

• Stable for long periods

See complete pipette decontamination procedure on page 83

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Cat. No. Item

725600 Biohit Carousel Stand for 6 mechanical pipettes

725610 Biohit Pipette Holder for one Proline Plus / mLINE pipette

721000 Carousel Stand for 5 Proline mechanical pipettes

725620 Linear Stand for all Biohit pipette models

* Includes Universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs)

Cat. No. Item

730981 eLINE Charging Stand for one pipette*

730991 eLINE Charging Carousel for 4 pipettes*

510001 Proline Charging Stand for one pipette*

511601 Proline Charging Carousel for 4 pipettes*

710999 ePET Stand for one pipette

When the pipette is not in use, it should be stored in an upright

position in order to avoid conta mination from dirty benches.

Biohit offers stands for all of its pipettes.

The stylish Biohit Linear Stand is designed for all Biohit

mechanical and electronic pipettes, and especially for mLINE,

Proline Plus, Proline mechanical and ePET electronic pipettes.

The stand is also compatible with a wide range of other pipette

makes.

The compact Carousel Stands are available in different models,

both for storage only and for charging.

Pipette Stands

eLINE Charging Carousel

eLINE Charging Stand

Proline Charging Carousel

Linear StandCarousel StandBiohit

Carousel Stand

Proline Charging Stand

ePETCharging Stand

Stands for mechanical pipettes

Stands for electronic pipettes

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Maxi-Volume Liquid Handling



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High performance pipetting aids for maximum volumes

Let the handling of larger volumes still be easy and lightweight by using Maxi-Volume

pipetting aids from Biohit! Biohit offers you a wide range of pipetting controllers and

fillers: Biofiller is a lightweight and easy-to-use pipette filler; Midi Plus and Midi Pro are

battery operated, rechargeable pipetting controllers; Prospenser Bottle-Top Dispensers

and Biotrate Digital Burette provide hassle-free and accurate dispensing.

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Feature Midi Plus

Batteries AA, 3 x 1.2 V, 270 mAh, NiCd

Charging batteries 14 hours for completely empty battery

Battery service life (from empty) Approx. 7 hours service life when fully charged

AC-adaptor Output voltage 9 V DC

Filters Hydrophobic 0.45 µm

Case material ASA + PC

Nose cone material PVDF (autoclavable)

Pipette holder Silicone (autoclavable)

Midi Plus Technical specifications

Midi Plus™ – for comfortable large volume pipettingBiohit Midi Plus is a lightweight cordless pipetting controller

which allows aspiration from bottles and tubes without the arm

and hand elevations required with serological or volumetric

pipettes. It fits all commonly used glass or plastic pipettes, but

can also be used with the Biohit 5 ml and 10 ml disposable tips.

The speed can be fine-tuned by applying varying finger pressure

to the operating buttons.

*Not available in units sold in the United States

Hydrophobic auto-clavable filter pre-vents over-aspiration

Fold-out stand*- Prevents pipette contamination- Saves space- Allows the unit to be rested on a table with pipette attached

Stepless speed control allows for the aspira-tion and dispensing speed to be adjusted easily and precisely to suit either large or small volume pipettes.

Feature Midi Plus™

Pipette types Plastic or glass 1-100 mlPasteur pipettes5 ml and 10 ml Biohit pipette tips

Rechargeable during use Yes

Adjustable speed Stepless speed control

Gravity dispensing Yes

Stand Attached support

Optional adapters for 5 & 10 ml pipette tips

Yes

Weight 207 g

Low battery indicator Yes

Autoclavable parts Nose cone, pipette holder and filter

Midi Plus Selection guide

Cat. No. Item Qty/Unit

710931 Midi Plus Pipetting Controller with Universal AC-adaptor*1

1

712912 Replacement filter, 0.45 µm, autoclavable

5

712913 Replacement filter, sterile, 0.45 µm 1

711015*2 Adapter set for 5 ml tip 1

711016*2 Adapter set for 10 ml tip 1

780300 Biohit tip 5 ml 100

780310 Biohit tip 10 ml 100

*1 Universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs)*2 Note: Adapter set includes both the nose cone (PVDF) and the silicon adapter.

Midi Plus & Accessories Ordering details

• Precise finger tip control of variable speed ranges

• Hydrophobic autoclavable filter prevents from overaspira-

tion

• Convenient fold-out bench stand supports the unit and

pipette when not in use*

• Linear speed control

• Low battery warning

• CE marked

• 2-year warranty Operating buttons

Fits all commonly used glass or plastic pipettes 1-100 ml

IVD

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71091X Midi Pro Pipetting Controller with AC-adaptor

1

721962 Replacement filter, 0.45 µm 5



721963 Replacement silicone pipette holder 1

Midi Pro & Accessories Ordering details

X: 1 = Euro AC-adaptor; 2 = U.S. AC-adaptor; 3 = U.K. AC-adaptor;4 = Japan AC-adaptor; 5 = Australian AC-adaptor (available only for MidiPro)

The Midi Pro is fitted with a 0.45µm filter and supplied complete with charger, two spare hydrophobic filters (1x 0.45µm and 1x 0.2µm), bench stand and instruction manual.

Operating buttons

Unique stand suitable for bench or wall mounting is supplied with the unit

Feature Midi Pro

Batteries 2 x 1.2V NiMH

Charging batteries 14 hours for completely empty battery

Battery service life (from empty)

500 – 1000 cycles

AC-adaptor Output 75/10 mA DC

Filters Hydrophobic 0.45 µm or 0,2 µm

Case material ASA

Nose cone material ASA

Pipette holder Silicone (autoclavable)

Midi Pro Technical specifications

Feature Midi Pro

Pipette types Plastic or glass 1-100 mlPasteur pipettes

Rechargeable during use Yes

Adjustable speed High and low speed modes

Gravity dispensing Yes

Stand Suitable for bench and wall mounting

Optional adapters for 5 & 10 ml pipette tips

No

Weight 180 g (without stand)

Low battery indicator Yes

Autoclavable parts Pipette holder

Midi Pro Selection guide

Midi Pro – to speed up large volume pipettingThe Midi Pro pipette controller delivers an efficient performance

with a powerful yet quiet motor to speed up pipetting of larger

volumes.

The Midi Pro fits the hand comfortably with the pipette holder

at an angle ideal for use within a laminar flow or microbiologi-

cal safety cabinet.

• Fills a 25ml pipette in under 3 seconds on its fastest setting

• Rechargable during use

• Powerful pump

• Low battery light

• Environmentally friendly nickel metal hydride battery

• Suitable for all types of pipettes 1-100ml

• Unique stand suitable for bench or wall mounting

• CE marked

• 2-year warranty

Selection of high or low modes allowing variable aspirate and dispense speeds (with blowout)

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Biofiller is a uniquely designed, lightweight and easy-to-use

pipette filler with an ultra-squeezable bulb to provide smooth,

manual pipetting control in both aspirating and dispensing,

using 1 ml to 100 ml pipettes.

Simply squeeze the large silicone bulb, and the thumb lever

controls both the aspirating and dispensing modes, with a

button to blow-out residual contents of pipette if required.


723039 Biofiller Pipette Filler with integral 0.45 µm filter

1

721963 Replacement silicone pipette holder 1




Biofiller Ordering details

Biofiller - for smooth manual pipetting

Midi Pro Technical specifications Blow-out button

Operating control

Biofiller offers:• Comfortable and simple to use

• Precise pipetting control

• Robust and lightweight

• Large capacity bulb

• Compatible with blow-out pipettes

• Simple thumb button operation

• Uses integral 0.45 µm, replaceable membrane filter to

ensure liquid is not accidentally drawn into the unit

• Autoclavable silicone pipette holder

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Prospenser Bottle-top Dispenser delivers safe and precise

dispensing of liquids including strong acids, bases and solvents.

It provides special features as easy-to-adjust calibration

mechanism and precision valve for enhanced accuracy and

usability.

• Dispensing directly from the supply bottle

• Easy to adjust calibration mechanism

• Fully autoclavable at 121˚C , 2 bar, 20 minutes

• Accuracy of delivery within ±0.3% on maximum delivery

• Chemically resistant fluid pathway

• Anti-drip precision valve mechanism ensures easy priming

and minimum waste with no leakage back into the

reservoir

• Easy to use volume adjustment for reproducible

dispensings

• Unlike other bottle-top dispensers, Prospenser’s glass

barrel can be disassembled from the pedestal for thorough

cleaning

• Wide range of adapters are included to fit the most

common bottle sizes

• Optional dispense tube extention allows fast and safe

dispensing even to narrow tubes

• Manufactured to ISO9002 standards, each unit is supplied

with an individual calibration certificate

Please note that the Prospenser is not supplied with a reservoir

Cat. No. Item Increment Max Volume Inacc. (%) Impr. (%)

723049 Prospenser 0.01–2.5 ml (with 38, 40 and 45 mm adaptors) 0.05 ml 2.5 ml 0.3 % 0.1 %

723050 Prospenser 0.1–5 ml (with 38, 40 and 45 mm adaptors) 0.1 ml 5 ml 0.3 % 0.1 %

723051 Prospenser 0.2–10 ml (with 38, 40 and 45 mm adaptors) 0.2 ml 10 ml 0.3 % 0.1 %

723052 Prospenser 1–30 ml (with 38, 40 and 45 mm adaptors) 1.0 ml 30 ml 0.3 % 0.1 %

723053 Prospenser 1–50 ml (with 38, 40 and 45mm adaptors) 1.0 ml 50 ml 0.3 % 0.1 %

721998 Dispense tube extension (see picture)

Prospenser Performance specifications and ordering details

Fully autoclavable

Easily removable PTFE piston for cleaning and smooth action

Easy to use volume adjustment

Precision valve mechanism ensures that the Prospenser stays fully primed all day

Set of Adaptors available

Borosilicate glass barrel protected with a transparent polypropylene sleeve

Easy to adjust calibration mechanism

Chemically resistant liquid pathway

Anti-drip tap valve

Bubble free dispensing

Optional dispense tube extension allows fast and safe dispensing even to narrow tubes

Prospenser Bottle-Top Dispenser with Anti-drip valve

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Anti-drip closure cap

Proline Prospenser has been designed for trouble-free and

reliable bottle-top dispensing of liquids, including concentrated

acids, bases, saline solutions, as well as many organic solvents.

• Dispensing directly from the supply bottle

• Fully autoclavable at 121˚C , 2 bar, 20 minutes

• Accuracy of delivery within ±0.5% on maximum delivery

• Chemically resistant fluid pathway

• Anti-drip closure cap included

• Easy to use volume adjustment for reproducible

dispensings

• Easy to dismantle for cleaning and maintenance

• Wide range of adapters are included to fit the most

common bottle sizes

• Optional flexible dispense tube extention (max length

800 mm) with safety handle permits fast and precise

dispensing even into narrow tubes

• Each unit is supplied with performance certificate and

tested according to ISO 8655

Cat. No. Item Increment Max Volume Inacc. (%) Impr. (%)

723045 Proline Prospenser 05-5 ml (with 25, 28, 32, 38 and 40 mm adaptors) 0.1 ml 2.5 ml 0.5 % 0.1 %

723046 Proline Prospenser 1-10 ml (with 25, 28, 32, 38 and 40 mm adaptors) 0.2 ml 5 ml 0.5 % 0.1 %

723047 Proline Prospenser 2.5-25 ml (with 32, 38 and 40 mm adaptors) 0.5 ml 10 ml 0.5 % 0.1 %

723048 Proline Prospenser 5-50 ml (with 32, 38 and 40 mm adaptors) 1.0 ml 30 ml 0.5 % 0.1 %

721633 Flexible dispense tube extension for 5 and 10 ml Proline Prospensers

721634 Flexible dispense tube extension for 25 and 50 ml Proline Prospensers

Prospenser Basic Performance specifications and ordering details

Fully autoclavable

Volume setting easy to adjust

Borosilicate glass barrel protected with a transparent polypropylene sleeve

Wide range of bottle adapters included

Chemically resistant liquid pathway

Proline® Prospenser - easy-to-use Bottle-Top Dispenser

Optional flexible dispense tube extension

• Coiled• Length 800 mm

Biotrate Digital Burettes

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The streamlined Biotrate digital burette and dispenser delivers

accurate, precise and convenient bottle-top titration, as well as

optimum operator safety.

• The head can be conveniently rotated 360º for operator

safety and comfort.

• See-through barrel ensures bubble-free titration.

• Automatic switch-off without losing the previous titrated

volume, when left unattended

• No dripping when in static mode

• A new safety pedestal valve allows the Biotrate fluid path

to be turned off manually to prevent accidental delivery.

Its right-angled spout is also designed to enhance safety in

use.

• Robust outer casing does not move during the priming or

dispensing sequences.

• PTFE plunger and borosilicate glass barrel allow

dispensing of aqueous solutions and some aggressive

liquids.

• Powered by a 3.6V lithium battery giving a minimum

life of 60,000 three-minute titrations. Automatic low

battery voltage is indicated on the large easy-to-read LCD

window.

Biotrate Digital Burettes 0–30 ml and 0–50 ml

Cat. No. Item Increment Inacc. (%) Impr. (%)

723054 Biotrate 0–30 ml (with 33, 38 and 45mm adaptors) 0.01 ml 0.2 % 0.1 %

723055 Biotrate 0–50 ml (with 33, 38 and 45mm adaptors) 0.01 ml 0.2 % 0.1 %

721998 Extendable delivery jet (see picture page 42)

Biotrate Performance specifications and ordering details

Simple user calibration and factory reset for ISO 9000 and GLP requirements

Visible borosilicate glass barrel protected with a transparent polypropylene sleeve allows inspection of liquid before dispensing

Chemically resistant and autoclavable liquid pathway

Bubble free dispensing

Anti-drip tap

Autoclavable liquid pathway

Please note that Biotrate is not supplied with reservoir

Continuous display of volume dispensed with zero reset feature for multiple titrations

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Custom-Made Solutions


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Yes, it is a true story. Biohit is a leading provider of tailor-made (OEM, Original Equipment

Manufacture) solutions to customers who require a little more from their liquid handling

devices. Thanks to our know-how, inventiveness and experience, we can take care of the

whole process from engineering concept to dispatch. Custom-made only for you.

IVD

Tailored solutions for customer-specific systems

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Biohit OEM Solutions – Safe, reliable and easy to useIn many applications, such as diagnostic test and analysis

systems, the accuracy of the results and reliability of the system

are mission-critical. This requires a lot from all components of

the system, also the pipette.

Biohit places a great emphasis on accuracy, precision, safety

and user-friendliness of its products. Its application-specific

solutions have been carefully manufactured according to the

requirements of international quality standards. As a customer

you can rest assured; Biohit solutions allow you to improve not

only the safety and reliability, but also the ease-of-use of your

system.

Custom-made for your needs

Biohit has developed different technologies that can be applied

in custom-made solutions. These include both electronic and

mechanical liquid handling pipette platforms. The Start mLINE,

eLINE, Proline and the new rLINE robotic dispenser module

families are easy-to-adapt platforms for your system. For more

specific needs, Biohit offers tailored solutions based on any of

its pipettes.

To learn more about Biohit’s OEM solutions, contact us at [email protected].

The Start mLINE is a pipette based on any mLINE model that is equipped with an on/off switch and cable. This can be used with coagulation analysers or any other instruments that require precise timing.

The RS eLINE platform enables the integration of the instrument and the pipette. A flexible cable between the instrument and the pipette provides both the power supply and the operational control via RS 232 communication. The solution is based on the Biohit single- and multichannel eLINE electronic pipettes.

For example, Pentapharm GmbH in Germany has developed a whole blood Point-of-Care coagulation system, the ROTEM® System, with the RS eLINE as a key component. The system is designed to reduce the risk of transfusion errors in critical surgery such as liver transplant, cardiac or vascular surgery, orthopaedic or trauma surgery.

Solutions for leading-edge companiesTailor-made solutions are a speciality of Biohit. Several leading-edge companies have for years been implementing variations of Biohit electronic and mechanical pipettes into their systems. Some of these internationally recognised companies have developed very specific applications with the R&D team of Biohit to exploit their own market niche.

The customer base includes global market leaders such as• Pentapharm (picture)• 3M• bioMérieux • Johnson & Johnson Group companies

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Biohit rLINE® – The ultimate OEM Dispenser Module The unique rLINE Robotic Dispenser Module has been

developed as an ideal front-end liquid handling tool for

robotic sample processing. This technology platform combines

numerous unique and innovative features, like electronic tip

ejection and Optiload tip pick-up to improve high quality

performance and functionality of sample processors and

automated instrumentation.

The rLINE Robotic Dispenser Module is offered as OEM platform

for tailored products ranging from 0.2 ml to 5 ml as single-

channel and 0.2 ml to 1200 ml as 8-channel. Additionally,

demo versions are available for evaluation and feasibility

purposes. A range of robotic tips is available on request.

The rLINE platform features and options: • Compact, space-saving design

• Fast and easy installation

• Zero cross contamination

• Integrated electronic tip ejector

• Versatile serial interface (RS-232 & RS-485 & CANBUS)

• Low voltage, low current consumption

• Liquid level sensor

• Micro volume dispensing options

For more information on rLINE and other customer-specific solutions, please visit www.biohit.com or contact Biohit at [email protected]

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Pipetting Academy

Pipetting Academy

™

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Training for better performance, ergonomics and safety

Are you concerned with the results of your work due to pipetting practices or RSI? Is your

hand, arm or back hurting when pipetting? Have you considered new or updated instru-

ments to improve your pipetting comfort or results? The Pipetting Academy seminar is

your key to better ergonomics, safety and pipetting results in your lab.

™

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Pipetting Academy

Why join the Pipetting Academy seminars?• Are you concerned with the results of your work due to

pipetting practices or RSI?

• Is your workstation comfortable and do you have the

right posture when pipetting?

• Have you considered that the pain in your arm and hand

may be related to the instruments or techniques you use?

• Do you know which technique to use with different types

of liquids?

• Do your results vary between different users?

• Does your equipment comply with tightening quality

regulations?

• Be guided through the essentials of laboratory

ergonomics

• Gain a better understanding of the ergonomic

risks in the laboratory environment and liquid

handling in particular

• Learn how to avoid these risks by choosing

the most appropriate working postures, liquid

handling devices and accessories

• Appreciate how you can contribute to savings

in both direct and indirect costs that are related

to bad ergonomics

• Better understand the influence of pipetting

techniques and environmental factors on the

testing results

• Be able to instruct your employees on all these

issues and make work not only more efficient

but also more enjoyable

• Get hands-on training to pipetting techniques

that will help you and your co-workers get

more accurate and precise results in the lab

What will you learn?

www.pipettingacademy.com

Pipetting should be easy and provide accurate results – but

often it can be tiring repetition causing RSI and inaccurate test

results.

To overcome these issues and to help you perform better with-

out compromises on your health and safety, we have developed

Pipetting Academy seminars with a comprehensive tutorial

material package. In the Pipetting Academy seminars you will

be able to increase your knowledge about ergonomics, safety

and pipetting performance in general.

The seminars are created by Biohit’s expert staff in cooperation

with customers and third party consultants, who have brought

together their experience and knowledge to promote better

pipetting.

To learn more about the Pipetting Academy and to participate,

contact your local Biohit representative.

At the Pipetting Academy seminars you will, among other

things, learn how to recognize the risk factors and how to select

the right tools and working postures.

The key topics will be delivered through easy-to-understand

videos and animations to visualize the important aspects of pi-

petting techniques and ergonomics at your work. These power-

ful tools aim to make an impact on your daily work, at your lab!

Pipetting Academy - for better health, safety and pipetting results

Seminars, How to attend

WATCH TRAILER ONwww.pipettingacademy.com

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Choosing the right seminarPipetting Academy offers you different seminars for different

purposes.

You may choose among:

Ergonomics

Learn about the optimal posture for pipetting and be familiar

with the tools that can help you work ergonomically and

efficiently. Know the risks and see the solutions.

Pipetting techniquesMaster your working tool. Handle the pipette correctly. Be guid-

ed through the several techniques your pipette is capable of.

Pipette calibrationLearn how to calibrate your pipette correctly to gain the most

accurate pipetting results.

ISO8655 quality standardsAll you need to know about the standards you face in your

everyday work.

• Seminars include both theory and practice!• Videos and ani-mations support learning

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Pipetting Academy

Get access to the educational material, videos and animations

Once you have signed up and participated the seminar,

you will automatically get access to educational videos,

animations and presentations about ergonomics, pipetting

techniques and calibration.

Through the animations you will be guided step by step in

correct handling of the pipette and in reverse pipetting, dilut-

ing and all other pipetting modes to make your work easier

and more efficient.

A Certified Professional Ergonomist will guide you through

the essentials in Ergonomics.

Presentations with explanations and illustrations about cali-

bration and quality standards are also available.

WATCH TRAILER ONwww.pipettingacademy.com

How to sign up for seminars?


Get certifi-cate of par-ticipation!

• To sign up for the seminar contact your local Biohit

representative

• The seminar is held in a location most suitable for

you and your colleagues

• The trainer will be certified by Biohit to hold the

Pipetting Academy seminar

• Each participate will receive a certificate of partici-

pation after the seminar

Participate in a seminar and get access to the

material on www.pipettingacademy.com

Screenshot from a Pipetting Academy animation

See you at Pipetting Academy!

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Did you know that...?• Pipetting over 1.5 h/day (300 h/year) exposes to RSI

(Repetitive Strain Injury)

• Lab professionals have twice as much WRULD (Work

Related Upper Limb Disorder) as employees in general

• 44 % of lab professionals suffer from disorders caused by

pipetting

• Pressing the plunger button as well as ejecting tips with a

conventional mechanical pipette together require a force

up to 70 N (7 kg), which corresponds to the force required

to lift 2 house bricks or 7 litres of milk

Comparison of pipetting work with electronic and mechanical pipettes Both the tip ejection force and the distance thumb has to travel to eject the tips have been measured.

mLINE and eLINE offer exceptionally light and ergonomic pipetting operations

– effortless accuracy• Light weight for easy liquid handling• Exceptionally light pipetting forces• Tip loading and ejection require only minimum force (Optiload)• Both left and right handed operation• Consistent results time after time

– leader in performance & ergonomics • Fully electronic pipetting operations• Multi-function device – a lot more pipetting modes than in a mechanical pipette• Less stages means time-savings especially in multi-dispensing (twice as fast as a mechanical pipette)• 30 times lighter pipetting than with a mechanical pipette• Unique electronic one touch tip ejection• Both left and right handed operation• User independent accuracy and precision

Mechanical Pipettes Electronic Pipettes

For easy and ergonomic manual pipetting, your first choice should be mLINE – as recommended by Health & Safety Officers of leading pharma ceutical companies.

When you need to pipette and dispense liquids as comfortably

as possible, your first choice should be eLINE. This Award

Winning pipette enables you to focus on your work instead of

worrying about Repetitive Strain Injury.

Follow the signs of the ergonomic pipette

Maximum comfort

Pay also attention to:• Weight and design of the pipette• Your seating position• Rest intervals• Bench layout & lighting• Number of repeated pipetting series

Clear & easy-to-read

display

Ergonomic handle design

Good finger support

Light weight

Light plunger movement

Light tip loading & ejection

Fast & light volume

adjustment

Optimal length

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Pipetting Academy

Pipetting with air displacement pipettes is one of the most

common laboratory tasks and includes a number of potential

risks. The risks can be divided into three defined areas:

1. Physical risk through stress (ergonomics)

2. Situations where the user is at risk of contamination

through handling of infectious or toxic agents

3. Situations where the sample or specimen is at risk through

contamination from other samples or environmental

factors.

Cleanliness is safety - protect your pipette and your sample from contamination

The user is often well protected by appropriate working

benches, seats, clothing and shields, but the pipette is forgotten.

Modern air displacement pipettes with disposable tips enable

fast and accurate pipetting, but contamination of the pipettes

may increase both the possibility of unreliable results and

health risks in laboratory work.

Organic and inorganic contamination can create problems for

the scientist. No matter whether the particles are viral, bacterial

ribonucleases or metal elements, in the testing environment they

may lead to erroneous results. It is the reduction in the numbers

of these particles and avoidance of cross contamination or

accidental addition to the sample or the equipment being used

that needs attention.

Corrosion of the pipette piston is another threat. The number

of corroded pistons that are replaced at service centres every

day, and pipettes with totally contaminated inner parts is

surprisingly high (see Figures A & C).

Finally, to ensure the safety, the laboratory worker should

also pay attention to the ergonomics at work (pipette and

environment), take care of the proper shielding and protection

(gloves, clothes) and follow good laboratory practice by

pipetting slowly and carefully to minimise aerosol formation

and foaming.

What can be done to enhance safety?

Fig A: Badly corroded and contaminated piston assemblies taken from a commonly used pipette

Fig B: Biohit pipette tip cone used with Biohit Safe-Cone Filter

Fig C: Tip cone taken from a commonly used brand of pipette after over-aspiration of blood-sample

Fig D: Clean tip cone

Fig A. Biohit mLINE features a unique tip cone filter ejection mechanism.

Fig B. The tip cone filter can be changed easily and safely with the use of forceps supplied with every Biohit pipette (excluding mLINE).

A B

Literature on www.biohit.com and page 94.


Biohit safety features include• Ergonomic pipette design for improved occupational health

• Accurate and precise results with the appropriate tools

• Cleanliness through Safe-Cone Filters, certified tips,

autoclavable pipettes and decontamination tools

• Appropriate storage with a wide range of pipettes stands

• High-quality after-sales services & accredited calibration

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Accuracy

It is possible to be very consistent, but consistently wrong.

Inaccuracy is the numerical difference between the mean

of a set of replicate measurements and the true value. High

accuracy, i.e., small inaccuracy means very little difference

between the mean sample and the true value. Accuracy is

achieved by careful calibration of a precise instrument.

Precision

Precision is an agreement between replicate measurements.

Precision has no numerical value, it is quantified by

imprecision. High precision, i.e., small imprecision means very

little variation between repeated measurements on the same

sample. To achieve it a precision instrument is required, but

also good laboratory practice must be followed.

When comparing the stepper motor technology and the DC-

motor technology for accuracy and precision, the dispensing

(d) mode is the best choice.

The DC-motor driven Biohit eLINE and a stepper motor driven

pipette were tested for linearity in the d-mode. The measurement

was done 5 times. The DC-motor driven Biohit electronic

pipette allows linear movement, - i.e. precise dispensing. The

tested stepper motor pipette generated a linear movement error,

which is equal to over ± 1.5 µl. This even exceeds the minimum

adjustable increment of the pipettes. In the d-mode, where even

dosing is important, this kind of an error is significant.

Biohit mLINE

Performance test of Biohit mLINE (violet line) and a conventional

pipette (blue line). Pipettes were tested over a 5-hour period

with sets of 5 repeat weighings of 500 microlitres 180 times.

The same trained operator was used. As shown, it is very hard

to meet the specifications (red line, ISO 8655-2) with a force

demanding pipette after prolonged pipetting hours, whereas

the light action of mLINE allows the same reproducibility over

the whole period.

Literature on www.biohit.com and page 94.

Ten times more precise dispensing with Biohit electronic pipettes – unbeatable linearity

One of the most common activities of laboratory workers is primary sample or reagent addition whilst performing a test. Some

laboratory workers perform thousands of tests daily. There are a number of factors that influence the pipetting performance. For

example, the quality of the products and tips, human error, pipetting techniques, and strain, as well as other factors, such as

environment (temperature, air pressure, humidity), type of sample and type of application.

The most difficult demand for accurate and precise pipetting results is the consistent handling and continual depression of the

plunger on pipettes.

How to get consistent results?

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22

0.08

0.06

0.04

0.02

0

-0.02

Erro

r (m

m)

Measuring points

Erro

r (m

m)

1 11 21 31 41 51 61 71 81 91 101 111

0.08

0.06

0.04

0.02

0

-0.02Measuring points

Consistent results time after time with Biohit mechanical mLINE pipettes

20 x 50 µl linearity measurement in d-mode.

DC-motor driven Biohit eLINE electronic pipette

Stepper-motor driven electronic pipette

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Pipetting Academy

Pipetting preparations

• Use the tip specified by the manufacturer.

• Ensure that the pipette and the tip have been tested according to ISO 8655 and the tip is seated correctly.

• Make sure pipettes have been correctly calibrated.

• Check that the pipette, tip and liquid are all at the same temperature.

• When pipetting liquids with temperatures different to the ambient temperature, do not pre-rinse the tip. Change the tip after each pipetting.

• Ensure that any fluid viscosity variations have been accounted for and the correct technique is employed, i.e. reverse pipetting.

• If handling infectious or radioactive agents make sure appropriate shielding and other precautions protect the operator. For information on safety accessories see pages 56–58.

• Use Safe-Cone Filter in the tip cone whenever possible.


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Pipetting action• Hold the pipette in a vertical position. Tilting the pipette at an angle causes a volume greater than the set volume of liquid to enter the tip.

• Pre-rinse of tip is always recommended.

• When aspirating fluid the pipette tip should normally be immersed to a depth of 2-3 mm.

• When using a mechanical pipette, operate piston with a smooth and consistent thumb action for repeated results without foaming or bubbles.

• It is recommended to pipette against the inside wall of the receiving vessel. Remove the tip by drawing it up against the inside wall.

• Ensure the pipette blow out action is fully activated.

• Ensure the volume is still set at the required position. Pipette with volume locking mechanism are recommended to avoid accidental volume change during pipetting.

• Avoid leaving the pipette on its side with liquid in the tip, which might seep back into the mechanism.

Other cautions• Rack the pipette when not in use – see product pages for more information on pipette stands. Electronic pipettes should be returned to their charging stands.

• Avoid dropping the pipette or allowing contact with dirt or grease.

• Change the Safe-Cone Filter regularly (recommendation after 50 to 250 pipetting cycles) and always in case of over-aspiration.

• Never strike the tip cone against the tip tray when loading the tip as this can damage the pipette.

• Avoid exposing the unit to extreme temperature changes, humidity and dust (operating temperature from 15°C to 40°C).

• Service the pipette regurarly.

• Clean the pipette thoroughly before sending to service. Decontaminating the pipette with Biohit Proline Biocontrol or 70% ethanol is a suitable procedure. Notify the service personnel for what purpose the instrument has been used. Postal services can refuse to deliver instruments used with hazardous materials. Make sure that a qualified person services the pipette.

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Pipette Calibration & Maintenance


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Beside pipetting, laboratories must pay attention also to the quality and performance

of the tools and equipment that are used in everyday work. To ensure the continuous

accuracy and precision of your liquid handling instruments we offer a wide range of

high quality services, such as quality control, repair services, preventive maintenance

and calibration services. And, when doing it regularly, you’ll keep your pipette in good

operating condition and also help extend its life-time.

Leading edge after-sales services by Biohit

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We provide preventive maintenance, repair, calibration and accredited calibration services through our global subsidiary and distributor network:

Through Biohit After-Sales Services you get• High quality maintenance, repair and calibration services

for all Biohit pipettes

• Good operating condition and extended life-time of

the pipettes thanks to scheduled preventive maintenance

programs and calibration services

• Cost savings with full spare part service with original spare

parts for Biohit pipettes

• Time savings through convenient and cost-efficient service

contracts, on-site services and fast turnaround times

• Detailed documentation on services performed

Global network of trained distributors and certified service centers

Biohit CertifiedService Center

Repair Service

PerformanceTesting

ProductAnd Service

Training

Full SparePart Service

AccreditedCalibration

Service

CalibrationService

PreventiveMaintenance

Service

3. Biohit Pipette Doctor Service Biohit Pipette Doctor Service Centers have expertise to

service and calibrate pipettes of all makes, all models,

and all volumes.

Pipette Doctor is the ultimate level of pipette service

within Biohit’s after-sales services and in the world. Pipette

Doctors are high performance service centers that have

deep technical knowledge and competence to service and

calibrate more than 800 different pipette models. Pipette

Doctor service is very practical if you have also other

than Biohit pipettes in your laboratory. Pipette Doctor

professional can service and calibrate them all. Advantages

are as follows:

• Professional one-stop pipette service for all makes

and models

• No need to send pipettes to many different locations

• Easy, simple and effortless service

• Cost savings through a single service contract for all

makes and models

• Please visit www.pipettedoctor.com for

your closest Pipette Doctor Service Center.

If such a service does not exist in your

area, please contact your Biohit distributor

for further actions

2.Biohit Certified Service Centers Biohit Certified Service Centers (BCSC) are highly

qualified for technical service and calibration. These

service centres are authorised, trained and regularly

audited by Biohit to offer you the highest quality of

service. They have also been tested and approved to

provide traceable calibration results by the Biohit

accredited (FINAS) calibration laboratory. The Biohit

Certified Service Centers maintain standard operating

procedures for technical service and calibration, test

equipment has been set up in accordance with ISO 8655

and the personnel have been carefully trained.

Please visit www.biohit.com for your nearest Biohit

Certified Service Center.

1. Technically trained distributors All Biohit distributors have been carefully trained to

maintain and calibrate liquid handling devices

manufactured by Biohit.

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Liquid handling devices, such as pipettes, are precision

instruments, that comprise a great variety of mechanical and

electronic parts. These parts are naturally subject to wear and

damage over time, for example:

• The piston surface can deteriorate, wear or corrode over

time.

• Tip cones are subject to chemical corrosion and wear,

especially in the tip sealing area.

• Batteries of electronic pipettes do not last forever and

must be replaced regularly.

All these problems can be overcome by carrying out regular

maintenance and calibration programs to ensure the

functionality and performance (inaccuracy and imprecision)

of the pipette. According to extensive experience and research

by Biohit, failure rates can be significantly reduced if a regular

and scheduled preventive maintenance program is carried out

on the pipettes. In addition, the pipette life-time can easily be

extended through regular service and replacement of critical

components.

Biohit offers scheduled Preventive Maintenance Programs for

service and calibration.

Biohit Preventive Maintenance and Repair Services

Procedure Benefits Suitable for

• Cleaning of the pipette

• Cleaning of the lower parts (ejector collars, piston, seal/o-ring, tip cone)

• Replacement of the critical parts, so- called Preventive Maintenance Packages if needed

• Re-greasing of the piston, seal or o-ring

• Pressure leakage test

• Calibration and performance testing according to the customer’s requirements (please see table on page 66)

• Detailed service documentation: service report and calibration report

• Improved pipette reliability

• Constant pipette performance level (inaccuracy and imprecision)

• Fewer failures due to unexpected malfunctions of the pipette

• Decreased pipette downtime as a result of preventive maintenance packages

• Longer lasting pipettes

• Lower number of pipettes that fail precalibration

• Less waste of time and money on exception reports

• Laboratories that have a strict quality system such as ISO 9001, ISO 17025, GLP or any other quality system imposed by local legislation, international standards or product/ service type.

• Laboratories that need to prove quality of their services or products to their customers.

• Laboratories following ISO 17025, - i.e. accredited laboratories

• Laboratories that are working with accredited laboratories but which are not necessarily accredited themselves

Biohit Preventive Maintenance Program

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• Product and technical service training is provided to customers who want to service and test their own pipettes in-house

• Biohit provides so-called preventive maintenance packages that include the most frequently used components for pipette service

• Your nearest Biohit distributor or Biohit Certified Service Center also provides the full range of original Biohit spare parts ensuring fast delivery. All spare parts are described and listed in instruction manuals and dedicated Technical Service Manuals

• Knowledge for service and test pipettes in-house

• Original spare parts

• Cost-effective

• Easy with preventive maintenance packages

• Customers who want to service and calibrate pipettes in-house: schools and universities, hospitals with technical maintenance departments etc.

• Customers who do not require traceable calibration of international measurement standards

Service portfolio listed above and offered by Biohit After-Sales Service centres may differ country by country. Please contact your local dealer for details.

Note: Before sending any instruments to technical

service, please ensure that they are freefromallconta-

mination. Please fill in decontamination certificate and

advise our Service Centers of any hazardous materials

which may have been used with your pipette.

For more information about Preventive Maintenance, Repair and Calibration services, please contact your local Biohit representative or go to www.biohit.com.


• Troubleshooting of the problem

• Disassembling the pipette: all parts are inspected for damage and wear

• Components are cleaned or replaced, whenever necessary

• Re-greasing of the piston, seal or o-ring

• Pressure leakage test

• Calibration and performance testing according to the customer’s requirements’ (please see table on page 66)

• Detailed service documentation: service repair report and calibration report

• Fully repaired and functional pipette

• Original spare parts

• Any customers with Biohit pipettes with damaged components or improper function and that need repair and calibration by qualified specialists

Biohit Repair Service

Biohit Maintenance by End-User & Full Spare Part Service

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The principles of Good Laboratory Practice (GLP) state that

laboratory equipment should be periodically inspected,

cleaned, maintained, and calibrated according to Standard

Operating Procedures (SOP). Records of these activities should

be maintained. Calibration should, where appropriate, be

The frequency of the testing and calibration depends on • Accuracy requirements of the application

• Frequency of use

• Number of operators using the pipette

• Nature of the liquid dispensed

• Specifications established by the user

• Requirements from GLP or quality systems

Calibration Servicestraceable to national or international standards of measurement.

Biohit is one of the few accredited pipette calibration labora tories

in the world today. To help you fulfil the GLP or quality system

requirements such as ISO 9001 and ISO 17025 we offer you:

Procedure What you get Suitable for

Pipette is tested for inaccuracy, imprecision and linearity to determine the uncertainties of measurement for each volume calibrated. Test volumes and data points are selected according to the customer requirements.

Accredited Calibration Certificate and uncertainty budget in accordance with ISO 17025. This is the highest level of calibration available.

Laboratories that are working with accredited laboratories or laboratories following ISO 17025, - i.e. accredited laboratories.

Calibration service portfolio listed above and offered by Biohit After-Sales Service centres may differ country by country. Please contact your local dealer for details. Please note: Test volumes 100%, 50% and 10% of the nominal volume are defined by ISO 8655:2002 standard and thus these volumes are also recommended by Biohit.


Basic checking of the inaccuracy and imprecision for the pipette: 5 readings measured at mid-volume (5x50%)

Performance Test Report This is a good basic “performance check” for your pipettes and can be used for laboratories working to GLP.


Pipette is tested for inaccuracy and imprecision and also for linearity: 5 readings measured at 10% of the maximum volume and maximum volume (5x100% and 5x10%)

Calibration Report detailing the inaccuracy and imprecision of each volume range tested

This calibration can be used if the pipette is used over the whole volume range. This can be used for GLP and as an entry level calibration for GMP as well.


Pipette is tested for inaccuracy, imprecision and linearity throughout its whole working range, including its mid-volume: 5 readings measured at its maximum, middle and 10% of the maximum volume (5x100%, 5x50% and 5x10%)

Calibration Report detailing the inaccuracy and imprecision of each volume range tested

This calibration can be used if the pipette is used over the whole volume range. This is suitable for customers working to GMP or laboratories where a higher level of confidence is required.


Pipette is tested for inaccuracy, imprecision and linearity throughout its whole working range, including its mid-volume: 10 readings measured at its maximum, middle and 10% of the maximum volume (10x100%, 10x50% and 10x10%)

ISO 8655 Level Calibration Report detailing the inaccuracy and imprecision of each volume range tested

10 readings at each volume ensure a high level of confidence. This is suitable for customers working to GMP or laboratories where a higher level of confidence is required.


Testing of the pipette prior to service and recalibration to establish how well the pipette has been performing “as found”. Same combination of data points as shown above.

Precalibration Report detailing the inaccuracy and imprecision of each volume range tested

Especially customers working to GMP

Basic Performance Testing (5.1)

Basic Calibration (5.2)

Enhanced Calibration (5.3)

ISO 8655 Level Calibration (10.3)

Accredited Calibration

Additional Service Precalibration (“As Found” Performance Test)

mLINE and Proline Plus Proline mechanical

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BIOHIT UNCERTAINTY BUDGET

Balance: Sartorius MC5, serial 11305654

Pipette: Biohit Proline 50 - 200 µl, serial AQ30836

50 µlytivitisneSdradnatS noitubirtsiDlavretnIretemaraP

)x(u*ctneiciffeocytniatrecnu -/+

balance uncertainty 8 µg rectangular 4,62 µg 1 nl/µg 4,62 nl

linearity 4 µg rectangular 2,31 µg 1 nl/µg 2,31 nl

1st value reproducibility 1 µg rectangular 0,58 µg 1 nl/µg 0,58 nl

2nd value reproducibility 1 µg rectangular 0,58 µg 1 nl/µg 0,58 nl

1st value resolution 1 µg rectangular 0,58 µg 1 nl/µg 0,58 nl

2nd value resolution 1 µg rectangular 0,58 µg 1 nl/µg 0,58 nl

temperature drift 1,00E-06 K-1 rectangular 5,77E-07 µg 1 nl/µg 5,77E-07 nl

water temperature 0,1 K rectangular 0,06 K 10 nl/K 0,58 nl

air temperature 0,1 K rectangular 0,06 K -0,23 nl/K -0,01 nl

pressure 10 hPa rectangular 5,77 hPa 0,06 nl/hPa 0,34 nl

relative humidity 10 % rectangular 5,77 % -0,01 nl/% -0,03 nl

delivering cubic

device expansion

coefficient 9,00E-05 K-1 rectangular 5,20E-05 K-1 -1,00E+05 nl*K -5,20 nl

temperature 2 K rectangular 1,15 K -4,5 nl/K -5,20 nl

standard uncertainty associated with the volume V20 9 nl

measured with the gravimetric measuring system

precision on the calibration 63 nl

standard uncertainty of the calibration 64 nl

Nowadays, many laboratories are accredited and work to strict

quality control guidelines and regulations. For this reason, the

calibration of laboratories’ instruments must be certified by an

independent body that is competent and qualified to perform

What you get Suitable for

Accredited Calibration Certificate (FINAS, ISO 17025) together with your

• new• serviced

Biohit pipettes.

Accredited Calibration Certificate supplied with your new or serviced Biohit pipette includes the Uncertainty Budget defining the uncertainties for the measurement involved.

As a result, you will know exactly what kind of uncertainty is due to the performance of the pipette and what is due for example, to the uncertainty of the balance.

Laboratories that are working with accredited laboratories or laboratories following ISO 17025, – i.e. accredited laboratories.

Ask for an additional Accredited Calibration Certificate from your nearest dealer next time you order a new pipette from Biohit. Price varies depending on pipette model.

Accredited calibration service for serviced pipettes is also available at Biohit UK (UKAS), Biohit France (COFRAC) and Biohit Germany (DKD).

service and calibration activities. Biohit is one of the few inter-

nationally accredited (ISO 17025) pipette calibration laborato-

ries in the world. We offer you accredited calibration for both

new and serviced Biohit pipettes.

An Accredited Calibration Certificate means that • Measurements are traceable to national and international

standards

• Calibration has been carried out to the highest standards

• Agreed and specified methods and procedures have been

followed

• The Biohit laboratory issuing the certificate has been

assessed by independent experts

Accredited Calibration Certificate (ISO 17025) for new and serviced Biohit pipettes

2-1513

Accredited Calibration Services

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As a leading designer and manufacturer of liquid handling products, Biohit recognises

the need to provide anyone who needs to check and calibrate pipettes, from end users to

pipette service companies, with a range of software to ensure the ongoing performance

of their pipettes. The Quanta range of software has been designed to offer a tailor-made

solution whatever your requirement for calibration and pipette service software.

Pipette Service and Calibration Software – Quanta

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Pipette Service and Calibration Software – Quanta

Biohit provides anyone who needs to check and calibrate

pipettes, from end users to pipette service companies, with

a range of software to ensure the ongoing performance of

their pipettes. Quanta schedules pipette calibrations, controls

and captures data from balances automatically, performs all

required calculations and produces reports such calibration

reports, service reports, overdue pipettes reports etc. Quanta

software is suitable for both single and multi-channel pipettes of

fixed and variable volume, manual and automatic dispensers,

burettes, dilutors and volumetric glassware.

Quanta range of software The suite of Quanta software:• Meets ISO 8655, DIN 12650, ISO 9000, ISO 17025 and

GLP requirements

• Can be used with your current calibration protocols

• Connects to any balance supporting RS232 interface;

controls and collects calibration data automatically from

the balance

• Converts automatically mass into volume by using

temperature and barometric pressure

• Provides automatic calculations on inaccuracy and

imprecision

• Produces calibration reports and optional reports

automatically

• Includes a database of 1500 pre-loaded test plans for all

major manufacturers pipettes and liquid handling

instruments

• Provides calibration specifications in accordance with

manufacturers specifications, ISO 8655:2002 maximum

permissible errors or user-defined specifications

• Allows you to service your pipettes via the software,

recording exactly what has been done to the pipette, what

has been fitted in the way of spares, what has been the

cost of maintenance etc.

• Maintains a complete service and calibration history

which can be accessed easily

• Provides full audit trail on service and calibration

measures - who, what, when and why

• Provides user assistance online (www.quantapro.net), user

wizard and user manual.

• Can be customized to your needs.

User-friendly and clear calibration interface

Instruments and equipment used within a laboratory need to

be routinely tested, validated and calibrated by laboratory

personnel or an external technically competent and authorized

calibration company.

Regularly checking the performance of your pipettes is vital to

ensure the ongoing quality of your results. End users may not

know that they are dispensing inaccurate or imprecise volumes

of liquid which in turn could lead to incorrect results. The more

frequently you test and calibrate your pipette, the sooner you

would detect these inaccuracies and imprecisions This will

maintain the integrity of your laboratory data and reduce the

likelihood of having to repeat your work.

Quality control in labs – why you need to do it

“Apparatus used in a study should be periodically inspected, cleaned, maintained, and calibrated according to Standard Operating Procedures (SOP). Records of these activities should be maintained. Calibration should, where appropriate, be traceable to national or international standards of measurement.”

(GLP, Good Laboratory Practice),

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FEATURES QuantaCal QuantaLab QuantaPro

Add/Edit Pipettes √ √ √

Personalised Information √ √ √

RS232 Balance Support √ √ √

Calibrate Pipettes √ √ √

Add/Edit Test Plans √ √ √

Multiple Departments √ √ √

Calibration Reports √ √ √

Multiple Customer Entry Optional √ √

Booking In/Dispatch System Optional √ √

Pipette Collection Requests * Optional √ √

Service Module Optional √ √

Spare Part Management ** Optional √ √

Due/Overdue Pipettes Report Optional √ √

Service/Calibration Recall Optional √ √

Email System Optional Optional √

Quality Control Optional Optional √

Report Editor Optional Optional √

Management Reporting Optional Optional √

Demo Equipment Management Optional Optional √

Quote Generator Optional Optional Optional

Mettler Multichannel Support Optional Optional Optional

Accredited Certificates (ISO17025) Optional Optional Optional

Multi-language Output Optional Optional Optional

Quanta Software Modules:

*Pipette Collection Request is a sub-module of the Booking in/Dispatch system. The Booking/Dispatch system has to be in place before installing Pipette Collection request. **Spare Parts Management is a sub-module of the Service Module. The Service Module has to be in place before installing Spare Parts Management

Modular structure – Quanta offers you what you need, not what you are given!

QuantaCal – Our entry level software. QuantaCal is designed

primarily for the end user that simply needs to maintain a

database of their pipettes, to be able check them against the

manufactures specification, ISO8655 or end user specification

and to produce a detailed calibration report that will satisfy

general GLP/GMP requirements.

QuantaLab – Our midrange software. QuantaLab is a unique

menu based software system, incorporating QuantaCal as

its base module and seven additional options (see table)

that enables you to control whole service and calibration

process and is ideally suited to the smaller service company

or organisation that tests and maintains their own pipettes in-

house.

QuantaPro – The most comprehensive service and calibration

software on the market. Developed for the professional high

output pipette service companies or large multi-site end user

accounts, it can develop all aspects of the service process from

receipt to dispatch.

The suite of Quanta software has been designed to offer a tailor-made solution whatever your requirement for service and calibration

software. The software has been developed in a modular way, enabling you to choose the options that you require to calibrate your

pipettes. Our standard tailored modules are as follows:

Please visit www.quantapro.net for further information on optional modules.

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CAT. NO. ITEM WHAT SUITABLE FOR

770020 QuantaCal Basic module of QuantaCal (see table on page 70)

End users and small service companies who wish to simply check pipettes on a regular basis

760005 QuantaLab Basic module of QuantaLab (see table on page 70)

End users and small/mid size service companies that need to have full control of service and calibration process

760002 QuantaPro Basic module of QuantaPro (see table on page 70)

High output service companies and demanding end users that require versatility from service and calibration software

770040 Validation package for Quanta Software

Installation, Operational and Performance Qualification documents (IQ, OQ and PQ)

Anyone who needs to validate calibration set up in accordance with prevailing standards

N/A For upgrades, optional modules and related catalogue numbers please see www.quantapro.net

Upgrade your software to your requirements

Ordering Details

Quanta software, developed by a professional pipette and service company, is truly the most comprehensive pipette service and calibration package available today.

How to order?• Please contact your local Biohit representative for

ordering information or

• Log on to www.quantapro.net and complete a

contact form which will be forwarded to your

nearest Biohit representative

Evalution VersionPlease download evaluation version from

www.quantapro.net or request a dvd from your Biohit

representative. Evaluation version allows for storing

information on and calibrating 25 pipettes.

Recommended System Requirements• 2GHz Pentium P4 Processor

• Windows: 2000/XP/Vista/Vista 64

• 256Mb RAM

• 100Mb Free Disk Space

Online Support and Additional InformationPlease visit www.quantapro.net for technical support

and further informationQuanta software produces individual calibration reports on each pipette tested

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Pipettes/Pipette decontamination procedure

1. Unscrew the tip ejector collar counterclockwise and

remove it.

Mechanical pipettes (mLINE + Proline Plus)

2. Unscrew the tip cone holder counterclockwise and

carefully remove it with the tip cone. Remove the Safe-Cone

Filter if fitted.

3. Unscrew the piston counterclockwise from the pipette.

4. Place the tip ejector collar, tip cone holder, tip cone and

tip cone cylinder into a beaker containing Biohit Proline

Biocontrol and leave for at least 30 minutes.

1. Unscrew the tip ejector collar counterclockwise and

remove it.

Electronic pipettes (eLINE)

2. Unscrew the tip cone holder counterclockwise and

carefully remove the tip cone holder, tip cone and spring.

Remove the Safe-Cone Filter if fitted.

3. Unscrew the piston counterclockwise from the pipette.

4. Place the tip cone, tip cone holder, tip ejector collar, piston

and spring into a beaker containing Biohit Proline Biocontrol

and leave for at least 30 minutes.

Note: When performing the decontamination procedure as a matter of routine, the o-ring seal should be checked for possible

wear at every sixth instance, and replaced if necessary. The calibration should also be checked at the same time.

5. After performing the procedure described above, remove

the components from the beaker and rinse them with distilled

water, then dry preferably with warm air, for at least an hour.

6. Re-grease the piston as described in the instruction manual.

Replace all components including new filter if fitted.

5. After performing the procedure described above, remove

the components from the beaker and rinse them with distilled

water, then dry preferably with warm air, for at least an hour.

6. Re-grease the piston as described in the instruction manual.

Replace all components including new filter if fitted.

Pipettes/

Pipettes/



Problem Cause Solution

Pipette performance outside given specs

• Unsuitable tip• Non-standard test conditions or calibration altered• Pipette has not been maintained regularly• Dirty Safe-Cone Filter• Pipette is leaking

• Test with original manufacturer´s tip• Perform test according to the ISO 8655 standard and recalibrate if necessary• Perform routine user maintenance and retest• Change Safe-Cone Filter• See instructions below

Pipette is leaking • Unsuitable tip• Tip incorrectly attached• Worn or dirty tip cone

• Worn or dried piston sealing

• Instrument damaged

• Use original manufacturer´s tip• Attach tip firmly• Clean tip cone• Change tip cone• Clean and re-grease o-ring• Change the o-ring• Send for Service

Operating button jammed or moves erratically

• Liquid has penetrated tip cone and dried inside• Safe-Cone filter has been contaminated• Insufficiant amount of grease on a piston and seal

• Clean and grease the piston/seal and the tip cone• Change Safe-Cone Filter• Grease accordingly

Pipette blocked, aspirated volume too small

• Liquid penetrated tip cone and dried • Clean and regrease o-ring and piston and clean tip cone

Tip ejector jammed or moves erratically

• Tip cone and/or ejector collar contaminated • Clean with soft cloth and mild detergent, Biohit Proline Biocontrol or 70% ethanol

Troubleshooting guide

mLINE & Proline Plus mechanical pipettesThe entire mLINE and Proline Plus mechanical pipette can be

steam sterilized by autoclaving at 121°C (252°F), 1 bar (15

p.s.i.) for 20 minutes. The dispensing head of the multichannel

pipettes must be unscrewed 360° counterclockwise before

autoclaving.

• Remove the Safe-Cone Filter (if fitted).

• Put the pipette into the sterilization bag and place it into

the autoclave.

• After autoclaving the pipette must be cooled down and

left to dry overnight before use.

It is recommended to check the performance of the pipette

after every autoclaving, and to grease the piston/seal of the

pipette after every 10th autoclaving.

eLINE electronic pipette, lower partThe dispensing head (tip ejector collar, tip cone holder, tip

cone, spring and piston) of the single-channel and multichannel

models (except for multichannel 1200µl) can be autoclaved

(121°C, 1 bar for 20 minutes). These parts can be autoclaved

as one unit or separately as individual parts. It is also possible

to clean the parts and grease the piston prior to autoclaving.

• Remove the Safe-Cone Filter (if fitted).

• Put the dispensing head into the sterilization bag

and place it into the autoclave.

• After autoclaving the parts must be cooled down

and left to dry before use.

It is recommended to check the performance of the pipette

after every autoclaving, and to grease the piston/seal of the

pipette after every 10th autoclaving.

Autoclaving instructions

Note:

• Excessive heat or length of time can damage the products.

Never put handle part of the eLINE into the autoclave.

• Lower ends of multichannel pipettes are not

interchangeable between 8- and 12-channel pipettes.

• The cover of the tray should be closed during

autoclaving.

Tips and tip boxes• Place the bulk tips into the

sterilization bag and the tip tray as

such in the autoclave.

• Autoclave for 20 minutes at a

temperature of 121°C under 1 bar

(15 p.s.i.).

• Cool down before use.


About BiohitBiohit was founded over 20 years ago, in 1988. The background

of the company, however, stretches back to the 1970s,

when Professor Osmo Suovaniemi (M.D, Ph.D.), founder of

Labsystems (1971), Eflab (1978) and Biohit (1988) together

with his team developed and commercialised the first air

displacement, adjustable single and multichannel pipettes that

made liquid handling both more convenient and safer than

before. These inventions included the first 8- and 12-channel

adjustable pipettes that were designed to aid the liquid

handling work in the vertical photometry based analysers,

such as the very first 96-well microplate reader (the Titertek

Multiskan), invented and developed by Professor Suovaniemi

(and his company Labsystems).

Over the years, Biohit’s liquid handling devices have taken their

place in laboratories worldwide as ergonomic, safe and reliable

solutions for all types of pipetting and dispensing.

Today, Biohit’s mission is to enhance the well-being and quality

of life of people by developing, manufacturing and marketing

liquid handling products and accessories as well as diagnostic

test systems for use in research, health care and industrial

laboratories.

In addition to liquid handling products and accessories, Biohit

also develops, manufactures and markets diagnostic test

systems for the screening, prevention, and diagnosis of diseases

of the gastrointestinal tract.

The company’s diagnostic tests are combined with pipettes,

instruments based on vertical photometry technology, and

related software to provide comprehensive analysing systems

for research and clinical diagnostics.

Biohit focuses on business areas in which it possesses a

strong foundation in research, technology, innovations and

patented products. The company’s products are designed and

manufactured according to the requirements of the ISO 9001,

ISO 13485, and ISO 14001 quality and environment standards.

Biohit also offers accredited pipette calibration according to

ISO 17025. Additionally, all products are CE/IVD marked.

Biohit has several worldwide patents and patents pending for

its products.

Biohit has a network of 200 distributors, including 8

subsidiaries, in 70 countries.

Further information on Biohit is available at www.biohit.com.


Our high quality standards

ISO 13485 quality standard is specific

to medical device quality systems and

supplements the more generic ISO 9001

standard that applies to many industries.

Additional ISO 13485 requirements include those that

are critical in the medical device industry, e.g. special

processes, design controls, process controls including

environmental, traceability, record retention and regulatory

actions.

ISO 8655 is an international quality standard for the

manufacture and testing of piston-operated volumetric

apparatus. ISO 17025 lists the general competence

requirements for carrying out tests and calibrations.

The IVD Directive (98/79/EC)1 is the third in

a series of European Directives on medical

devices. The purpose of the IVD Directive is to

ensure that only safe and functional products

are sold in the European market, with clearly

outlined regulations regarding manufacture, importing, and

marketing. The CE/IVD mark stands for excellent quality

and purity and thus guarantees maximum reproducibility

for experiments and analyses.

Ergonomic Design – Improving ergonomics

of pipette design and usability to reduce the

risk of work related upper limb disorders,

such as RSI (Repetitive Strain Injury). Biohit

is the global market leader in electronic

pipettes, which combine performance and ergonomics to

provide accurate, reproducible and safe pipetting in the

most demanding liquid handling applications.

Tip purity - All tip models available as DNase,

RNase and Endotoxin free certified to protect

the sample from contamination. Biohit’s tip

production is ISO 8 cleanroom classified.

Warranty – Biohit offers 3-year warranty for

all mechanical pipettes and 2-year warranty

for electronic pipettes. The low lifetime

cost of the products, warranty periods and

environmentally friendly products allow for

highreturnoninvestment.

FINAS is the Finnish national

accreditation body, which

operates independently as part

of the Measurement Technology Center (MIKES), and

who has given Biohit’s calibration laboratories in Finland

accreditation certificate. An accredited pipette calibration

laboratory calibrates pipettes according to precise technical

requirements. Our calibration laboratories in Germany,

France and the UK have obtained the same status by their

national accreditation bodies.

ISO9001•ISO13485•ISO14001•ISO17025

Biohit’s products are developed and manufactured according to the requirements of ISO

9001, ISO 13485 and ISO 14001 quality and environment standards. The tip production also

follows ISO 14644-1 Standard to fulfill ISO class 8 cleanroom conditions. Our accredited

pipette calibration follows ISO 17025. The products are CE/IVD marked and supplied with

individual QC certificates.

We aim to continuously develop our products and processes in order to meet – and

often exceed – the demands of regulatory authorities, environmental bodies and most

important, our customersmost important, our customers.

IVD


Literature

Ergonomics

• Vaughton, R. (2006) In case of emergency – break glass! Int. Labmate. (2006)

• Mannonen, S., Nieminen, P., Kaasinen, J., Andersin, K. (2004). Multichannel pipetting – how to

choose the correct pipette? Int. Biotech. Lab. 22(6), 12-14 and Am. Biotech. Lab 22(13), 12-14

• Mannonen, S., Nieminen, P., Kaasinen, J., Andersin, K. (2003). Raising the standard of mechanical

pipetting. Int. Labmate 28(5), 34-35.

• Mannonen, S., Hintikka, V. and Syrjä, K. (2002). Choosing the correct electronic pipette. Clin Lab

Int. 26(2), 29.

• Mannonen, S., Hintikka, V. and Syrjä, K. (2002). The benefits of electronic pipetting. How to choose

the correct pipette. Int. Labmate 27(4) Guide 2002/2003, 75-77.

• Mannonen, S., Hintikka, V., Kaasinen, J. and Ekholm, P. (2002). Reduced tip ejection and pipetting

forces prevent repetitive strain injuries (RSI). Biomed. Prod. 4, 40-42.

• Vaughton R. (Sep. 1999). Taking the Strain Out of Pipetting. International Biotechnology Laboratory.

• Hoskins D.B., Erickson J. (1998). Laboratory Ergonomics, the Wake-Up Call: A Case Study on How

One Company Relieved Stress and Strain on Its Employees, Chemical Health and Safety, January/

February.

• David G., Buckle P. (1997). A Questionnaire Survey of the Ergonomic Problems Associated with

Pipettes and Their Usage with Specific Reference to Work-Related Upper Limb Disorders. Applied

Ergonomics, Vol. 28, No. 4: 257-262.

• Hodgson E. (1996). Work Related Upper Limb Disorders and the Laboratory. World Directory of

Environmental Testing, Monitoring and Treatment.

• Fredriksson K. (1995). Laboratory Work with Automatic Pipettes: A Study on How Pipetting Affects

the Thumb, Ergonomics 38 (5): 1067-1073.

• McGlothlin J.D., Hales T.R. (1995). NIOSH (National Institute of Occupational Safety and Health)

Health Hazard Evaluation Report.

• Björkstén M.G., Almby B., Jansson E.S. (1994). Hand and Shoulder Ailments among Laboratory

Technicians Using Modern Plunger-Operated Pipettes. Applied Ergonomics 25: 88-94.

Safety

• Mannonen, S., Riikonen, S. (2006) In Vitro Diagnostics (IVD) compliance and what it means in practice.

Am. Biotech. Lab. 24(8), 18–20.

• Mannonen, S. (2005) Use of a certified pipette tip to protect samples. Am. Biotech Lab. 23(8), 23-24.

• Mannonen, S. (2005) Protecting your sample - Using a certified pipette tip. Int. Biotech. Lab. 23(3),

10-12.

• Mannonen, S. (2005) Pipette tip cone filters minimise the risk of contamination. Labasia 12(4), 24-25;

and Int. Labmate 30(5), 15-16.

• Mannonen, S. and Hintikka, V. (2004). Poorly selected tips can cause major error to pipetting accuracy.

Int. Labmate. 29(2), 55-56.

• Mannonen S., Syrjä K. (2000). Safety in Pipetting. International Labmate, 25(1), 39.

• Mannonen S., Tiusanen T., Suovaniemi O. (2000). Major Sources of Error of Air Displacement Pipettes.

Lab Asia, 7(2), 38-39.

• Kolari M., Mannonen S., Takala T., Saris P., Suovaniemi O., Salkinoja-Salonen M.S. (1999). The Effect of

Filters on Aseptic Pipetting Lifetime of Mechanical and Electronic Pipettes and Carryover during

Pipetting. Letters in Applied Microbiology 29: 123-129.

Service & Maintenance

• Hemmings, Ian (2009). The importance of Regular In-Lab Checking of Pipettes.

• Mannonen S., Riikonen S. (2000). Accredited Calibration and Future Demands for Pipettes.

International Biotechnology Laboratory, 18(2), 48-49.

For literature see also www.biohit.com.


Liquid Handling

Diagnostics

More publications and pdf of all publications can be downloaded at www.biohit.com

Brochures

Corporate

GERMANY Biohit Deutschland GmbHRaiffeisenstrasse 161191 Rosbach, GermanyTel: +49-6003-82 820Fax: +49-6003-828 [email protected]

JAPANBiohit Japan Co., Ltd.NB Building 6F2-15-10 Iwamoto-cho, Chiyoda-kuTokyo, 101-0032 JapanTel: +81-3-5822 0021Fax: +81-3-5822 [email protected]

INDIABiohit Biotech Systems (India) Private LimitedNo.10, Anna Avenue, Bhakthavatsalam Nagar,Adyar, Chennai 600020, India

U.K.Biohit Ltd. Unit 1, Barton Hill WayTorquay, Devon TQ2 8JG,United KingdomTel: +44-1803-315 900Fax: +44-1803-315 [email protected]

FRANCEBiohit SAS2 Rue du Grand Chene78830 Bonnelles, FranceTel: +33-1-3088 4130Fax: +33-1-3088 [email protected]

USABiohit Inc.3535 Route 66, Bldg. 4P.O. Box 308, Neptune, N.J. 07754-0308, U.S.A.Tel: +1-732-922 4900Fax: +1-732-922 [email protected]

RUSSIABiohit OOO, Saint-PetersburgVasiljevskij ostrov, line 5 -68 building 4, letter D 199178 Saint-Petersburg, RussiaTel: +7-812-327 5327Fax: +7-812-327 [email protected]

Biohit OOO, MoscowPetrovsko-Razumovsky av. 29, building 2103287 Moscow, RussiaTel: +7-495-614 9550Fax +7-495-613 [email protected]

CHINABiohit Biotech (Suzhou) Co Ltd.Room 501, Office BlockHotel Equatorial65 Yan An Xi LuShanghai, 200040P. R. ChinaTel: +86-21-6248 5589Fax: +86-21-6248 [email protected]

Contact details

For a list of distributors world wide go to www.biohit.com

Biohit – Finnish innovation, design and manufacture for laboratory professionals

490069.05 • 1/2010 ©

Biohit O

yj

All information contained in this publication is current at the time of publication and whilst the greatest care has been taken over its compilation, Biohit is not responsible for any errors or omissions and reserves the right to make changes without notice. All trademarks are Biohit property unless otherwise stated. Patents granted or pending.

HEADQUARTERSBIOHIT OYJ, Laippatie 100880 Helsinki, FinlandTel: +358-9-773 861Fax: +358-9-773 86 200E-mail: [email protected]

Biohit Catalogue 2010

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Transcript of Biohit Catalogue 2010