min0 5 10

mAU

0

50

100

150

200

250

300

350DAD1 B, Sig=292,8

Ref=off (VAV0613\007-0901.D)

2.7

69 3

.129

3.4

49

7.1

59 7

.866

min0 5 10

mAU

0

50

100

150

200

250

300

350

400 DAD1 B, Sig=292,8Ref=off (VAV0613\006-0801.D)

2.76

23.

125

3.44

2

7.85

1

10.7

08

(a) (b)

0 2 4 6 8 10 12 14 16

Time (min)

0

10

20

30

40

50

60

70

80

90

100R

elat

ive

abun

danc

e

8.65

5.59

10.4

7

14.5

0

9.52

16.2

2

13.0

8

11.9

4

6.62

2.92 3.86

4.79

NL:3.64E7TIC MS2008-620

(a)

RT: 0.00-17.12 SM: 7G

8.03

8.63

5.58

10.4

6

14.4

8

16.6

9

15.7

1

13.0

7

9.51

11.6

9

6.61

3.71

4.78

0 2 4 6 8 10 12 14 16

Time (min)

0

10

20

30

40

50

60

70

80

90

100

Rel

ativ

e ab

unda

nce

NL:3.31E7TIC MS2008-619

(b)

RT: 0.00-17.16 SM: 7G

2.29

2-as

p2.

587-

glu

6.14

5-hi

s

4.48

1

5.35

8-se

r

6.91

6-gl

y7.

332-

thr

8.81

8-ar

g9.

604-

ala

12.0

89-t

yr

18.1

32-t

rp

19.0

32-[

he19

.464

-ile

20.7

69-l

eu

21.9

79-l

ys

28.1

64-p

ro

15.3

94-c

ys-s

16.3

54-m

et15

.977

-val

25

20

15

10

5

0

-5

Norm.

0 5 10 15 20 25 min

WWD1 A, wavelength = 338nm, TT (20080603\060330.D)

(a)

2.29

3-as

p 2.59

7-gl

u

5.35

7-se

r6.

148-

his

6.92

0-gl

y7.

345-

thr

9.60

9-al

a8.

821-

arg

12.0

91-t

yr

15.3

97-c

ys-s

16.9

83-v

al16

.358

-met

18.1

29-t

rp

19.0

26-p

he19

.458

-lie

20.7

58-l

ue

21.9

64-l

ys

28.1

52-p

re

0 5 10 15 20 25 min

17.5

15.0

12.5

10.0

7.5

5.0

2.5

0.0

-2.5

-5.0

Norm.WWD1 A, wavelength = 338 nm, TT (20080603\060331.D)

(b)

50

40

30

20

10

0

Tra

nsm

ittan

ce (%

)

3340

.26

2925

.92

1651

.37 15

36.2

7 1450

.94

1315

.94

1071

.97

780.

59

(a)

70

60

50

40

30

20

10

0

Tra

nsm

ittan

ce (%

)

3336

.22 29

57.2

6

2926

.97

1655

.39 15

35.4

0 1401

.06

1237

.66

1075

.55

4000 3000 2000 1000

Wave no. (G1)

(b)