Basics: TMA · What is TMA? (1) •Combines tens to hundreds of paraffin embedded tissue specimens...
Transcript of Basics: TMA · What is TMA? (1) •Combines tens to hundreds of paraffin embedded tissue specimens...
Basics: TMA
Ákos Ágyi
Technical sales representative
What is TMA? (1)
• Combines tens to hundreds of paraffin embedded
tissue specimens into a single paraffin block
• Tissue cores are extracted from one or more
representative regions of a paraffinembedded
tissue block (donor block)
• Then precisely arrayed into a new “recipient”
paraffin block
What is TMA? (2)
• Tissue microarray
How to make a TMA Block? (1)
How to make a TMA Block? (2)
• Manual moulding
• Manual punching
• Time consuming
• Low density TMA
How to make a TMA Block? (3)
How to make a TMA Block? (5)
How to make a TMA Block? (4)
How to make a TMA Block? (6)
• Computer controlled
• Drilling
• Punching
• Sample insertion
• Fast and precise
• High density TMA
TMA sectioning and staining
Analysis of TMA slides (1)
• Computer image
scoring
• Easy comparison of
tissue samples
• Digital imaging help
to alleviate previous
bottlenecks, in the
research pipeline
Analysis of TMA slides (2)
Applications of Tissue microarrays (1)
• All the histochemical
and molecular detection
techniques (developed
for regular sections)
works with TMA
sections
Applications of Tissue microarrays (2)
• In cancer research:
• Prevalence TMAs
• analyze the frequency of a molecular alteration in different tumor types
• Progression TMAs
• study the role of a marker in tumor initiation, progression, or metastatic growth
• Prognosis TMAs
• contain tissues with patient follow-up data.
• TMAs are used for many noncancer applications:
• Alzheimer's research
• Inflammatory disease research
Applications of Tissue microarrays (3)
• Clinical pathology workflow, ex. routine diagnostics
of breast pathology
• Immunohistochemistry quality control and quality
assurance
• Biomarker discovery and validation processes
• Discovery of potential molecular targets for gene-
specific therapy
Possible customers for TMA products
• Bio-banks
• Research laboratories
• Pathology departments in hospitals
• Pharmaceutical companies
TMA Laboratory equipment from 3DHISTECH
Tissue Microarrayers
Tissue preparation tools
Digital slide scanners and TMA analysis software
Tomorrow morning, 09:15 - 10:00
• Recent developments
in TMA Grand Master
and TMA Master
control software
Thank you for your kind attention!