Basics of Chrom

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  • Basics of ChromatographySeparation of AnalytesType of Equilibrium

  • Basics of ChromatographyAbility to dissolve in mobile phaseInteraction with stationary phaseTime on X axisResponse on Y

  • Chromatography Types

  • Separation dependent on time on the column (along with other factors)

  • Retention time of the analyteRetention time of the Mobile Phase

  • Basics of ChromatographyAverage linear flow rateAverage linear flow of mobile phase

  • Plate Height Theoretical Measure of Efficiency

  • Fig. 26-6Determining standard deviation from a peakStd dev = W = 4

  • Basics of ChromatographyCapacity FactorSelectivity Factor

  • Basics of Chromatography

    The Van Deemter Equation

    H = A + B/u + Cu

  • Multiple Flow PathsTortuosity

  • Components of the VanDeemter

  • Van Deemter Plot

  • How Plate Height is effected by particle size in solid packing

  • Basics of ChromatographyColumn Efficiency

  • Resolution

  • Basics of ChromatographyResolution

  • The Effect of Mobile Phase on Retention

  • More on Resolution