Potential uses of Stem Cell

Potential uses of Stem Cell

African Journal of Internal Medicine Vol. 1 (1), pp. 010- 016, 2012. International Scholars JournalsFull Length Research PaperAutologus MSC bone marrow stem cell and allogenic pancreatic stem cell for repair of beta pancreatic cell in experimental diabetes mellitusPurwati Armand1,2, Fedik. A. R2, Sony Wibisono1, Anas. P2, Eric. H2, Helen. S2, Deya. K21Department of Internal Medicine, Faculty of Medicine, Airlangga University Dr. Soetomo Hospital, Surabaya, Indonesia.2Stem Cell Laboratory, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia.*Corresponding author. E-mail: purwatipanpan@yahoo.comReceived 23 October, 2012; Accepted 19 December, 2012AbstractAlternative therapies in diabetes mellitus (DM) management include the use of stem cells. Stem cells derived from the bone marrow and pancreatic cells from allogenic donors were used in this experimental animal model to restore glucose control. DM was induced in Wistar rats with 50 mg/kg alloxan. DM rats were divided into 4 treatment groups, Group 1 was transplanted with autologous bone marrow derived mesenchymal stem cells (MSC) by intraperitoneally injection. Group 2 was given allogenic pancreatic cells intraperitoneally. Group 3 was given insulin subcutaneously, and Group 4 served as control (no treated). The dosage was 200,000 cells/rat. Post therapy results in group 1 revealed significant decrease of blood sugar levels, an increase in insulin levels, and increased C peptide levels. In group 2, there were more pronounced changes, improvements in glucose control compared to group 1. Those receiving only insulin the levels of blood sugar decreased but less so compared to those receiving MSC or pancreatic cells (p = 0.002). In a DM Wistar rat model the intraperitoneal administration of pancreatic cells resulted in better restoration of glucose control than intraperitoneally of bone marrow derived MSC, which in turn was better than only insulin.Key words: Diabetes mellitus (DM), stem cell, allogenic, autologous. Induced Monocytes derived HSCs (CD34+) with LPS accelerated Homing Rat-Bone Marrow Mesenchymal (CD105) in Injured Pancreas 1,2Fedik A. Rantam, 1,3Purwati, 6Budi Setiawan, 3Sony Wibisono.,1,4Ferdiansyah.,1.5Joni Wahyuhadi., 1.4 Edward Mouly., 1.4 Dwikora N. Utomo., 1.4 Heri Suroto 1 Stem Cell Lab. Institute of Tropical Disease, Airlangga University Indonesia; 2Virology and Immunology Lab., Dep. of Microbiology, Faculty of Veterinary Medicine, Airlangga University; 3 Tropic and Infection Division of Internal Medicine, School of Medicine, Airlangga University; 4Regenerative Medicine, Department of Orthopedic, Dr. Soetomo Teaching Hospital/ School of Medicine Airlangga University Surabaya; 5 Dep. of Neurosurgary, Dr. Soetomo Teaching Hospital/ School of Medicine Airlangga University Surabaya; 6Animal Surgary, Animal Hospital, Faculty of Vet. Medicine Airlangga University, Surabaya-Indonesia. ABSTRACT Investigating the function of combining induced rat monocytes derived bone marrow-haemopoietic stem cell (rat BM-HSCs) with LPS and rat bone marrow- mesenchymal stem cell (rat BM-MSCs) was to use for acceleration of homing process mechanism in injured pancreas. Mononucleate stem cells were isolated from aspirated whole rat BM using ficoll and cultured in -MEM complete growth medium in 10 cm petridish. Two days, adherents cell after twice washing in petridish were added -MEM growth medium and then mesenchymal cells were characterized using CD105 marker in third passage. Then for haemopoietic Stem Cell (HSCs) were isolated from rat bone marrow with magnetic beads CD34+ and differentiated in vitro then induced monocyte with LPS. Animal experiments were used 28 male wistar rat, and devided into 4 groups. After transplantation with combined stem cells were analyzed the expression of pair box gen 4 (Pax4), pancreatic and duodenal homeobox (Pdx1), c-Peptide used immunocytochemistry and immunohistochemistry, and secreted insulin, c-peptide used indirect ELISA. Results showed that the expression of Pax4, Pdx1, c-peptide found in the surface membrane cell of pancreatic cell, and secreted c-peptide and insuline showed significant P