Automating SPE Extractions with the HT400E System
description
Transcript of Automating SPE Extractions with the HT400E System
Automating SPE Extractions with the HT400E System
by
William AmoyalDisruptive Technologies
Disruptive Technologies history
• Distribution company founded in 2005• Based in Villecresnes, France• Cover France, Belgium, Switzerland and other
countries depending on product range• Agilent Service Partner for UV/Vis
spectrophotometry, HPLC and CE• 2 people
– William Amoyal, Sales Manager– Franck Do Vale, Service Manager
Disruptive Technologies product range
• Sample Preparation– Nucleic acides, protein and
small molecules extraction from hard-to-lyse tissue samples
– Online SPE system (couples with LCMS)
• Dissolution/Formulation– Dissolution baths, friability and
disintegration instruments• Physico Chemistry
– HT Log P and pKa analyzer– HT oligonucleotides purity
analyzer– HT proteins analyzer– HT DNA analyzer– HT Chiral analyzer– Kinetic solubility instruments
• Service– Agilent Channel Partner to
service their range of HPLC, UV Spectroscopy and CE
• Rapid microbiology with Micro PRO
• Analytical/preparative– OPLC chromatography
solutions for semi preparative applications (OPLC systems, pumps, sample applicator, video imaging and densitometry instruments, reagent sprayer.
– Spotter for MALDI and tissue MALDI imaging
– ELSD detectors– Type-C silica hydride HPLC
columns Flat sorbent beds for OPLC
– Accessories and consumables for CE and HPLC
– Validation kits for HPLC systems
Benefits of HT400E system
• Reliable– Based on reliable technology of HTA autosampler already
used by Agilent, Shimadzu, Perkin etc ... In their GC systems
• Reproducible– Very low CV% inter-assay <2,5%
• Flexible– Accepts many types of SPE cartridge, sample and target
vials
• User friendly– Intuitive “drag-and-drop” software
• Competitive Price– Highest cost-to-performance SPE system available on the
market
HT400E system with cooling unit
Main features of HT400E system
• Can be used as an SPE system or HPLC autosampler
• 20/29 samples max per tray
• 4 solvents available (more on request – max 11)
• Syringe and needle washing
• Software manager QUICK-PREP
• Sample tray Peltier cooling unit (optional)
Sample tray configurations
• 20/29 samples per TRAY
• Sample volume: ∞ ml
• SPE– 1ml , 3ml standard– 6 ml optional
• Target Volume:– 2 ml– 4 ml
• Syringe:– 1 ml– 2.5 ml
• Other on request
Ideal workflow
• Cartridge activation– Volume, n. of solvent, n. of cycles
• Cartridge drying– # of cycles
• Sample loading– Volume, # of cycles
• Sample washing– Volume, # of solvent, # of cycles
• Elution– Volume, # of solvent, # of cycles, different target vials
• Reagent addition– Volume, # of reagent, different target vials
• Washing syringe and needle– # of cycles
• Post-washing cartridge to re-use it
Easy-to-use Quick-Prep software
• Drag-and-drop software– Tools such as Draw, Dispense,
Mixing, Elution, Drying, Cartridge Picking, Cartridge Carry, Injection, Valve Washing, Cartridge Conditioning etc ... can be dragged and dropped in method making it possible to write methods in minutes
• Easy and intuitive software
• Easy set-up of the workstation (WorkTable)
• Easy-write work-up process (WorkFlow)
• Possibility to link methods
Application examples and results
• The following evaluation has been made using the VMA kit of Chromsystems
• The procedure has been as requested by Chromsystems protocol
Chromatogramme of VMA separation
Minutes
0 2 4 6 8 10 12 14
mV
olts
0
100000
200000
300000
400000
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600000
mV
olts
0
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2,68
7 V
MA
3,68
3 is
o-V
MA
7,24
3
10,4
83 5
-HIA
A
13,8
17 H
VA
ECD - Analog Board 2
Retention TimeName
Reproducibility of VMA separation
Retention Time Area Name ISTD concentration Units
2,685 2177549 VMA 4,670 mg/L
3,680 3811619 iso-VMA 0,000 mg/L
10,478 4300813 5-HIAA 5,066 mg/L
13,837 3194021 HVA 4,826 mg/L
1 2 3 4 5 6 7 8 AVR. S.D. R.S.D
VMA 4,67 4,87 4,64 4,58 4,92 4,78 4,73 4,67 4,73 0,117 2,47%
5-HIAA 5,05 4,98 4,98 4,72 4,91 4,82 4,81 5,06 4,92 0,123 2,50%
HVA 4,83 4,66 4,87 4,73 4,74 4,73 4,64 4,83 4,75 0,083 1,75%
Carry-over on pathological control
Minutes
0 2 4 6 8 10 12 14
mV
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400000
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800000
mV
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2,5
85 V
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3,5
00 i
so
_V
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3,9
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9,0
50 5
-HIA
A
12
,172 H
VA
13
,043
EC Detector - Analog Board 2
Retention TimeName
Retention Time Area Name ISTD concentration
Units
2,585 6645979 VMA 14,150 mg/L
3,500 3968477 iso_VMA 0,000 mg/L
9,050 9851053 5-HIAA 15,390 mg/L
12,172 7550284 HVA 12,711 mg/L
Pathological Control
Carry-over on water
Retention Time Area Name ISTD concentration
Units
2,585 6645979 VMA 14,150 mg/L
3,500 3968477 iso_VMA 0,000 mg/L
9,050 9851053 5-HIAA 15,390 mg/L
12,172 7550284 HVA 12,711 mg/L
Minutes
0 2 4 6 8 10 12 14
mV
olts
0
100000
200000
300000
400000
500000
mV
olts
0
100000
200000
300000
400000
500000
3,4
97 iso
_V
MA
EC Detector - Analog Board 2
Retention TimeName
WATER
Specifications
HPLC
Sample volume: Fixed loop
Syringe sizes: 0.5ml, 1ml, 2.5ml, 10ml (2.5ml as standard)
Derivatization time: User defined
Derivatization function: Choice of reagents
Needle wash: With solvent/sample
Loop wash: With solvent/sample
HPLC tray option: 110 sample positions
SPE
SPE cartridge volumes available: 1ml, 3ml
Tray capacity: 29 tubes (20 as standard)
Tray temperature control option: Peltier system. 10°C below ambient to +40°C
Solvent lines: Up to 10 (4 as standard), plus one wash solvent
Physical Characteristics
Dimensions (W x H x D): 510 x 735 x 400mm
Mass: 15.0kg
Power: 110/220V AC, 50/60Hz
For more information on HT400E system
• To see a video, click on picture besides in Diaporama mode or copy following URL in your browser
http://www.youtube.com/watch?v=XApeb60kGUs
• To download a brochure click on or copy following URL
HT400E SPE system brochurehttp://wamoyal.free.fr/LabHut/SPE/HT400E%20SPE%20system.pdf
Contact details at Disruptive Technologies
Contact
William Amoyal, Sales Manager
Disruptive Technologies
3 Allée des Camélias
94440 Villecresnes
Mobile: +33 (0)6 98 64 98 81
Email: [email protected]
Web: www.disruptechno.com