Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format –...
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Transcript of Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format –...
Automated Immunoassays
• Multi-test immunoassays are the most widely used rapid test format – worldwide– ELISA / EIA (Assurance EIA, TECRA)– ELFA (Vidas)
• Benefits– Low cost/test– Low labor cost with automation– Capable of high throughput– Validated, proven technology
Full automation of EIA procedure
Sample dispensing
Reagent delivery
Washing
Incubation
Photometric reading
Results interpretation
BioControl EIA AutomationBioControl EIA Automation
Continuous Loading
Bi-directional LIMS Compatibility
High Throughput Up to 186 samples and controls per run Continuous loading
Highly adaptable open platform• Pre-programmed for TRANSIA® PLATE• Pre-programmed for Assurance EIA®
• Compatible with all EIA assays
BioControl EIA Automation BioControl EIA Automation OptionsOptions
• BioControl EIA’s
Final Enrichment
Transfer 1 mL to Cluster tubes
Inactivationheating block
Load tubes and ReagentOn Gemini
• Vidas
Final Enrichment
Transfer 0.5 mL toVidas Test Strip
InactivationHeating Block
Load Test Strips andSPRs on Vidas
Co
mp
ara
ble H
and
s-On T
ime
Comparison of Through-put
Throughput of 1 Gemini = 3 Vidas Systems
Gemini Vidas
8 h 480 samples 168 - 280 samples
24 h 1600 samples 560 - 896 samples
Gemini vs VidasCapability Gemini Vidas Winner
Max Capacity 288 samples / controls
30 samples / controls
Gemini
Continuous Loading Yes No Gemini
Multiple Assays per Run Yes Yes – limited by capacity
Gemini
Hands-on Time Minimized by high sample
capacity
Increased by low sample
capacity
Gemini
Individual Run Time Approx 2 hr Approx 1 hr Vidas
Overall Run Time Minimized by high sample
capacity
Increased by low sample
capacity
Gemini
Calibration 1 / year 28 / year Gemini
Barcode Reader Yes Yes Equivalent
LIMS Capability Yes Yes Equivalent
• Automates each and every step
WASH
Washer • 8 channels • Aspirate / dispense needles• 3 wash buffers • Customized wash patterns
PIPET
Pipettor • Disposable tips• Capacitive liquid level detection• Pipetting pressure monitoring• 300 µL tips (10-300 µL) – Samples• 1100 µL tips (301-1000 µL) - Reagents
• Automates each and every step
INCUBATE
2 incubator chambers • Independently controlled• Shaking capacity• Temperatures up to 45°C
3 ambient temperature positions
Unique modular design •Easy service & Maintenance•Minimizes down time•Reduces repair costs
READ
Absorbance reader • 400-700 nm wavelength• Single or dual wavelength• Range 0.0 – 3.0 OD
• On-board Computer
Computer Ports • 3 USB ports for external peripheral
keyboard, mouse, printer, etc…• VGA monitor port • LAN cable connection• PS2 port
Touch-Screen Interface• Full function user interface
• Automates each and every step and MORE!
Multiple Assays• Same plate –common protocol• Different plates – common protocol or
different protocols
Continuous loading of resources• Plates and tips• Samples, and reagents• Instrument will show when Plate 3 can be loaded
Continuous operation• Initiate new assays during run
• Flexible to meet your high throughput needs
Accepts individual sample tubes or cluster tubes
12 loading bay lanes• Accepts both sample and reagent racks
Accepts reagent bottles directly from kit
• No reagent transfer
Automated resource tracking• Reagent volume tracking• Disposable tip tracking
Resource verification• Are reagents present • Sufficient reagent volume• Sufficient tips
Automatic schedule optimization• Multiple plates / assays• Addition of new samples/ assays
Assurance EIA Pathogen TestsAssurance EIA Pathogen Tests
• TRANSIA® PLATE Salmonella Gold
• TRANSIA® PLATE Listeria
• TRANSIA® PLATEListeria
monocytogenes• TRANSIA® PLATE
Staphylococcal Enterotoxins
TRANSIA PLATE EIA Pathogen TRANSIA PLATE EIA Pathogen TestsTests
*TRANSIA® is also available for Allergen and Chemical Residue detection