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M. Amit, J. Itskovitz-Eldor, Technion – Israel Institute of Technology, Haifa, Israel (Eds)

Atlas of Human Pluripotent Stem CellsDerivation and Culturing

Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research.

Features 7 Provides up-to-date techniques in the field of embryonic and induced pluripotent stem cells 7 Commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass blastocyst stage embryos 7 Describs the principles of karyoty-ping and fluorescent in situ hybridization (FISH) methods in the field of pluripotent stem cells

Contents Methods for the Derivation of Human Embryonic Stem Cell Lines.- Morphology of Human Embry-onic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders.- Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Condi-tions.- Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Sus-pension and in Dynamic Cultures.- Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bo-dies.- Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation.- Immunostaining.- Ka-ryotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines.- Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes.

Fields of interestBiological Microscopy; Stem Cells; Cell Biology

Target groupsProfessional/practitioner

Discount group P

 

Due December 2011

2012. X, 154 p. 105 illus., 88 in color. (Stem Cell Biology and Regenerative Medicine) Hardcover7 $139.00ISBN 978-1-61779-547-3

9   

R. B. Ashman, University of Queensland, Brisbane, QLD, Australia (Ed.)

LeucocytesMethods and Protocols

Contents ENU Based Phenotype Driven Screening.- Detec-tion and Quantification of Cytokines and Other Biomarkers.- FLOW CYTOMETRY ANALYSIS OF CELL CYCLING AND PROLIFERATION IN MOUSE HEMATOPOIETIC STEM AND PRO-GENITOR CELLS.- Flow Cytometry Measure-ment of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo.- Ana-lyzing Cell Death Events in Cultured Leukocytes.- Towards a four-dimensional view of neutrophils.- Isolation of Human and Mouse Neutrophils Ex vivo and In vitro.- MEASUREMENT OF OXIDA-TIVE BURST IN NEUTROPHILS.- Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates.- The Macrophage.- Gene-ration and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice.- Generation of Mouse Bone Marrow-derived Macrophages.- Iso-lation and Differentiation of Monocytes-macro-phages from Human Blood.- In Vitro Measure-ment of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages.- Measuring the Inflammasome.- Arginine and Macrophage Acti-vation.- Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation.- Detection of Human and Mouse Granzyme B Activity in Cell Extracts.- T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation.- Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry.

Fields of interestCell Biology; Developmental Biology

Target groupsProfessional/practitioner

Discount group P

 

Due January 2012

2012. XVI, 320 p. 62 illus., 24 in color. (Methods in Molecular Biology, Volume 844) Hardcover7 $119.00ISBN 978-1-61779-526-8

9   

M. L. Badenes, Instituto Valenciano de Investigaciones Agrarias (IVIA), Valencia, Spain; D. H. Byrne, Texas A&M University, College Station, TX, USA (Eds)

Fruit BreedingFruit Breeding is the eighth volume in the Hand-book of Plant Breeding series. Like the other volu-mes in the series, this volume presents informati-on on the latest scientific information in applied plant breeding using the current advances in the field, from an efficient use of genetic resources to the impact of biotechnology in plant breeding. The majority of the volume showcases individual crops, complemented by sections dealing with important aspects of fruit breeding as trends, mar-keting and protection of new varieties, health be-nefits of fruits and new crops in the horizon. The book also features contributions from outstanding scientists for each crop species.

Features 7 Presents the latest scientific information in applied plant breeding 7 Features the current advances in the field, from an efficient use of genetic resources to the impact of biotechnology in plant breeding 7 Illustrated with both detailed line drawings and color photos

Contents Trends in Fruit Breeding.- Developing Fruit Cultivars with Enhanced Health Properties.- Intellectual Property Protection and Marke-ting of New Fruit Cultivars.- Emerging Fruit Crops.- Blackberry.- American Cranberry.- Gra-pe.- Raspberry.- Strawberry.- Apple.- European Pear.- Apricot.- Cherry.- Peach.- Plum.- Citrus.- Persimmon.- Almond.- Chestnut.- Pecan.- Pista-chio.- Walnut.

Fields of interestPlant Genetics & Genomics; Plant Sciences; Agriculture

Target groupsResearch

Discount group P

Due December 2011

2012. XV, 825 p. 83 illus., 41 in color. (Handbook of Plant Breeding, Volume 8) Hardcover7 approx. $239.00ISBN 978-1-4419-0762-2

9   

www.springer.com/978-1-61779-547-3www.springer.com/978-1-61779-526-8www.springer.com/978-1-4419-0762-2

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E. Biancardi, Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Rovigo, Italy; L. W. Panella, USDA-ARS, Fort Collins, CO, USA; R. T. Lewellen, USDA-ARS, Salinas, CA, USA

Beta maritimaThe Origin of Beets

Along the undisturbed shores, especially of the Mediterranean Sea and the European North Atlantic Ocean, is a quite widespread plant called Beta maritima by botanists, or more commonly sea beet. Nothing, for the inexperienced observer‘s eye, distinguishes it from surrounding wild vegeta-tion. Despite its inconspicuous and nearly invisible flowers, the plant has had and will have invaluable economic and scientific importance. Indeed, ac-cording to Linnè, it is considered „the progenitor of the beet crops possibly born from Beta mariti-ma in some foreign country“.  Recent molecular research confirmed this lineage. Selection applied after domestication has created many cultivated types with different destinations. The wild plant always has been harvested and used both for food and as a medicinal herb.

Features 7 Narrative experiences of researchers that have investigated the sea beet populations around the world and worked with the wild plant 7 One-of-a-kind text entirely devoted to sea beet 7 Illus-trated with both detailed line drawings and color photos

Contents Foreword.- Preface.- Acknowledgments.- History and current importance.- Range of distribution.- Morphology, physiology, and ecology.- Taxono-my.- Uses.- Source of useful traits.- Cultivated offspring.- The Future.- Appendix.

Fields of interestPlant Sciences; Plant Breeding/Biotechnology; Plant Anatomy/Development

Target groupsResearch

Discount group P

Due December 2011

2012. XVIII, 312 p. 126 illus., 81 in color. Hardcover7 $209.00ISBN 978-1-4614-0841-3

9   

A. C. Brand, D. M. MacCallum, University of Aberdeen, UK (Eds)

Host-Fungus InteractionsMethods and Protocols

Contents Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy.- Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans.- Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans.- Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi.- Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers.- Multiple Gene Deletion in Cryptococ-cus neoformans Using the Cre--lox System.- Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast.- Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach.- Gene Disruption in Coccidio-ides Using Hygromycin or Phleomycin Resistance Markers.- RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum.- RNA Interference in Cryptococcus neoformans.- Gene Knock-Down in Paracoccidioides brasilien-sis Using Antisense RNA.- Tetracycline-Inducible Gene Expression in Candida albicans.- Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii.- Modular Gene Over-Expression Stra-tegies for Candida albicans.- Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans.- Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans.- Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils. [...]

Fields of interestMicrobiology; Pathology; Laboratory Medicine

Target groupsProfessional/practitioner

Discount group P

 

Due January 2012

2012. XVII, 598 p. 104 illus., 34 in color. (Methods in Molecular Biology, Volume 845) Hardcover7 $159.00ISBN 978-1-61779-538-1

9   

J. M. Dunwell, A. C. Wetten, The University of Reading, UK (Eds)

Transgenic PlantsMethods and Protocols

Contents Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation.-Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed.-Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants.-Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii.-A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.).-Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene.-Visual Selection in Rice—a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products.-Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System.-Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments.-Agrobac-terium-mediated Transformation of Sorghum bicolor Using Immature Embryos.-Split-transgene Expression in Wheat.-Agrobacterium-mediated Transformation of Brachypodium Distachyon.-Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules.-Biolistic Mediated Production of Transgenic Oil Palm.-Transformation of Oil Palm using Agrobacterium tumefaciens.- Highly Effici-ent Transformation Protocol for Plum (Prunus domestica L.).-Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes.-Initiation and Transforma-tion of Grapevine Embryogenic Cultures. [...]

Fields of interestPlant Sciences; Plant Genetics & Genomics; Transgenics

Target groupsProfessional/practitioner

Discount group P

 

Due February 2012

2nd ed. 2012. X, 574 p. 96 illus., 53 in color. (Methods in Molecular Biology, Volume 847) Hardcover7 $159.00ISBN 978-1-61779-557-2

9   

www.springer.com/978-1-4614-0841-3www.springer.com/978-1-61779-538-1www.springer.com/978-1-61779-557-2

Life Sciences. springer.com/NEWSonline

80

R. C. Elston, Case Western Reserve University, Cleveland, OH, USA; J. M. Satagopan, Memorial Sloan-Kettering Cancer Center, New York, NY, USA; S. Sun, Case Western Reserve University, Cleveland, OH, USA (Eds)

Statistical Human GeneticsMethods and Protocols

Contents Genetic Terminology.-Identification of Geno-type Errors.-Detecting Pedigree Relationship Errors.-Identifying Cryptic Relationships.-Estimating Allele Frequencies.-Testing Departure from Hardy-Weinberg Proportions.-Estimating Disequilibrium Coefficients.-Detecting Familial Aggregation.-Estimating Heritability from Twin Studies.-Estimating Heritability from Nuc-lear Family and Pedigree Data.-Correcting for Ascertainment.-Segregation Analysis using the Unified Model.-Design Considerations For Gene-tic Linkage And Association Studies.-Model-based Linkage Analysis of a Quantitative Trait.-Model-based Linkage Analysis of a Binary Trait.-Model-free Linkage Analysis of a Quantitative Trait.-Mo-del-free Linkage Analysis of a Binary Trait.-Single Marker Association Analysis for Unrelated Samples.-Single Marker Family-based Association Analysis Conditional on Parental Information.-Single Marker Family-based Association Analysis not Conditional on Parental Information.-Al-lowing for Population Stratification in Association Analysis.-Haplotype Inference.-Multi-SNP Haplo-type Analysis Methods for Association Analysis.-Detecting Rare Variants.-The Analysis of Ethnic Mixtures.-IDENTIFYING GENE INTERACTION NETWORKS.-Structural Equation Modeling.-Genotype Calling for the Affymetrix Platform.-Genotype Calling for the Illumina Platform. [...]

Fields of interestAnimal Genetics and Genomics; Human Genetics; Biostatistics

Target groupsProfessional/practitioner

Discount group P

 

Due January 2012

2012. XV, 538 p. 102 illus., 45 in color. (Methods in Molecular Biology, Volume 850) Hardcover7 $159.00ISBN 978-1-61779-554-1

9   

G. S. Hall, Cleveland Clinic Foundation, Cleveland, OH, USA (Ed.)

Interactions of Yeasts, Moulds, and Antifungal AgentsHow to Detect Resistance

The incidence of fungal infections increases with the increase in antibiotic usage and increasing immunosuppressed populations. There is no lon-ger only one antifungal agent  and the response of fungi to various agents is not always predictable. The need for standardized antifungal susceptibility testing, and standardized interpretation of results in conjunction with studies that describe clinical outcomes based on those tools is ever important. Interactions of Yeasts, Moulds, and Antifungal Agents: How to Detect Resistance covers the available antifungal agents, how to perform in vitro testing and  how those results should be in-terpreted for the most common fungal pathogens.

Features 7 References about antifungal agents and how to test for their resistance and interpret re-sults 7 Standardized up-to-date methods for susceptibility testing 7 Provides a clear overview of the basics

Contents Antifungal Agents.- Antifungal Susceptibility Testing: Clinical Laboratory and Standards Insti-tute (CLSI) Methods.- Antifungal Susceptibility Testing Methods – Non CLSI Methods for Yeast and Moulds.- Susceptibility Testing of Dermato-phytes.- Usual Susceptibility Patterns of Common Yeasts.- Usual Susceptibility Patterns of Common of Moulds and Systemic Fungi.- Usual Susceptibi-lity Patterns for Systemic Dimorphic Fungi.- Utili-ty of Antifungal Susceptibility Testing and Clinical Correlations.

Fields of interestMicrobiology

Target groupsResearch

Discount group P

 

Due December 2011

2012. XII, 138 p. 2 illus. in color. Hardcover7 $139.00ISBN 978-1-58829-847-8

9   

J. S. Hartig, University of Konstanz, Germany (Ed.)

RibozymesMethods and Protocols

Contents Introduction.-Characterization of Hammerhead Ribozyme Reactions.-Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Me-thods for RNA Preparation, Structure Mapping, Solvent Isotope Effects, and Co-transcriptional Cleavage.-Kinetic Characterization of Hairpin Ribozyme Variants.-Characterization of RNase P RNA Activity.-Group I Intron Ribozymes.-Kinetic Characterization of Group II Intron Folding and Splicing.-Mechanism and Distribution of glmS Ribozymes.-Structure-based Search and in vitro Analysis of Self-cleaving Ribozymes.-Discovery of RNA Motifs using a Computational Pipeline that Allows Insertions in Paired Regions and Filte-ring of Candidate Sequences.-Crystallographic Analysis of Small Ribozymes and Riboswitches.-Functional Dynamics of RNA Ribozymes Studied by NMR Spectroscopy.-Deoxyribozyme-based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a Macrolide Antibiotic Resistance Peptide.-Probing Functions of the Ribosomal Peptidyl Transferase Center by Nucleotide Analog Interference.-Single Mole-cule FRET Characterization of Large Ribozyme Folding.-Metal Ion-RNA Interactions Studied via Multinuclear NMR.-Analysis of Catalytic RNA Structure and Function by Nucleotide Analog Interference Mapping.-In Vitro Selection of Metal-Ion-Selective DNAzymes.-In vitro Selection of Allosteric Ribozymes.-Screening Effective Target Sites on mRNA: A Ribozyme Library Approach.-A Computational Approach to Predict Suitable Tar-get Sites for Trans-acting Minimal Hammerhead Ribozymes.-Targeting mRNAs by Engineered Sequence-specific RNase P Ribozymes. [...]

Fields of interestBiochemistry, general

Target groupsProfessional/practitioner

Discount group P

 

Due January 2012

2012. X, 537 p. 102 illus., 27 in color. (Methods in Molecular Biology, Volume 848) Hardcover7 $139.00ISBN 978-1-61779-544-2

9   

www.springer.com/978-1-61779-554-1www.springer.com/978-1-58829-847-8www.springer.com/978-1-61779-544-2

News 11/2011 Life Sciences.

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V. M. Heine, VU University Medical Center, The Netherlands; S. Dooves, D. Holmes, J. Wagner, VU University Medical Center

Induced Pluripotent Stem Cells in Brain DiseasesUnderstanding the Methods, Epigenetic Basis, and Applications for Regenerative Medicine.

Brain diseases can have a large impact on patients and society, and treatment is often not availa-ble. A new approach in which somatic cells are reprogrammed into induced pluripotent cells (iPS cells) is a significant breakthrough for regenerative medicine.

Features 7 Up-to-date review on the latest reprogramming techniques 7 Comprehensive overview of the epigenetic models and how these will help us to map cell identity, plasticity and fate 7 Current state and issues on induced pluripotent stem cells in cell therapies for brain diseases

Contents I. Introduction.- II. Cell Reprogramming: A New Era in Regenerative Medicine.- a. Stem Cells.- b. Reprogramming Methods.- c. Measuring Pluripo-tency, Efficiency, and Identity.- d. Induced Pluri-potent Stem Cells versus Embryonic Stem Cells.- III. Understanding Epigenetic Memory is the Key to Successful Reprogramming.- a. Pre-fertilization to Embryo.- b. Epigenetic Control in De-, Re-, and Trans-differentiation.- IV. Prospects for Cell Replacement Therapies for Brain Diseases.- a. Parkinson’s Disease.- b. Childhood Brain White Matter Disorders.- V. Conclusions.- VI. Acknow-ledgement.- VII. References.

Fields of interestStem Cells; Neurology; Pediatrics

Target groupsResearch

Discount group P

Available

2012. XI, 71 p. 7 illus., 6 in color. (SpringerBriefs in Neuroscience) Softcover7 $49.95ISBN 978-94-007-2815-8

9   

A. Hübel, T. Schmelcher, U. Storz, Duesseldorf, Germany

Biopatent Law: Patent Strategies and Patent ManagementPatents protecting biotechnological invention are becoming ever more important. Because biotech-nology has many differences with respect to other technologies, lessons learned in other fields of technology cannot simply be transferred to adopt a suitable strategy for dealing with biotechnology inventions. In this volume, general aspects of bio-patent law will be discussed. This involves ques-tions of patentability, including ethical issues and issues of technicality, as well as questions of patent exhaustion in cases were reproducible subject matter, like cells or seeds, is protected. Moreover, active and passive patent strategies are addressed. Further, insight will be given into patent lifetime management and additional protective measures, like supplementary protection certificates and data exclusivity. Here, strategies are discussed how market exclusivity can be extended as long as possible, which is particularly important for biopharmaceutical drugs, which create high R&D costs.

Features 7 Improves the understanding of patent issues 7 Written by internationally active ex-perts 7 Provides a clear overview of the basics of patent management and strategies

Contents Andreas Huebel: General Issues of Biotech Patents.- Thilo Schmelcher: Active and Passive Patent Strategies.- Ulrich Storz: Patent Lifetime Management, SPCs and Data Exclusivity.

Fields of interestCell Biology; Pharmaceutical Sciences/Technolo-gy; Microbiology

Target groupsResearch

Discount group P

Available

2012. XII, 44 p. 1 illus. in color. (SpringerBriefs in Biotech Patents) Softcover7 $49.95ISBN 978-3-642-24845-0

9 

C. López-Larrea, A. López Vázquez, B. Suárez Álvarez, Hospital Universitario Central de Asturias, Oviedo, Spain (Eds)

Stem Cell TransplantationOrgan transplantation has been the most impor-tant therapeutic advance in the last third of the 20th century.

Features 7 Focuses on the guidance cues and molecular pathways regulating HSC trafficking throughout the lifetime of the organism 7 Summarizes some ongoing approaches of stem cell regenerative medicine 7 Introduces recent findings from published studies and clinical trials

Contents The Frontiers of Organ Transplantation and Cell Therapy.- Organ Transplantation in the 21st Century.- Immunology and the Challenge of Transplantation.- Cellular Immunotolerance in the Transplant.- Immunosuppression in the Era of Biological Agents.- Transgenic Organs and Xenotransplants.- Cell and Tissue Therapy in Regenerative Medicine.- Characteristics of Adult Stem Cells.- Bone Marrow Transplantation Extends its Scope.- Biology of Stem Cells: The Role of Microenvironments.- Mobilization and Homing of Hematopoietic Stem Cells.- Great Promise of Tissue-Resident Adult Stem/Progenitor Cells in Transplantation and Cancer Therapies.- Multipotent Mesenchymal Stromal Cells: Clinical Applicat Ions and Cancer Modeling.- Neural Stem Cells and Transplantation Studies in Parkinson’s Disease.- Biological Impact of Humanembryoni C Stem Cells.- Epigenetics of Embryoni C Stem Cells.- New Tools in Regenerative Medicine: Gene Therapy.- Therapeutic Cloning and Cellular Re-programming.- Advances in Stem Cell Therapy.- Tissue Bioengineering and Artificial Organs.

Fields of interestStem Cells; Cancer Research

Target groupsResearch

Discount group P

Due February 2012

2012. XXVI, 340 p. 48 illus., 17 in color. (Advances in Experimental Medicine and Biology, Volume 741) Hardcover7 approx. $209.00ISBN 978-1-4614-2097-2

9   

www.springer.com/978-94-007-2815-8www.springer.com/978-3-642-24845-0www.springer.com/978-1-4614-2097-2

Life Sciences. springer.com/NEWSonline

82

E. M. Sigurdsson, New York University School of Medicine, NY, USA; M. Calero, Instituto de Salud Carlos III, Madrid, Spain; M. Gasset, Consejo Superior de Investigaciones Cientificas, Madrid, Spain (Eds)

Amyloid ProteinsMethods and Protocols

Contents Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-redox Cycling.-Appli-cation of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins.-PREPARATION OF STABLE AMY-LOID β-PROTEIN OLIGOMERS OF DEFINED ASSEMBLY ORDER.-Purification and Fibrilla-tion of full-Length Recombinant PrP.-Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies .-Quasielastic Light Scattering Study of Amyloid b -Protein Fibrillogenesis.-Conformations of Microtubule-Associated Protein Tau  Mapped by Fluorescence Resonance Energy Transfer (FRET).-Measuring the Kinetics of Amyloid Fibril Elongation using Quartz Crystal Microbalances (QCM).-X-ray Fibre Diffraction Studies of Amyloid Fibrils.-Structural Characterization of Pre-fibrillar Intermediates and Amyloid Fibrils by Small-angle X-ray Scattering.-Atomic Force Fluorescence Mi-croscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates.Investiga-ting Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy.-Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR.-Cyclic Amplification of Prion Protein Misfolding .-Search for Amyloid Binding Proteins by Affinity Chromatography.-Using Biophysical Approaches for Establishing the Links Between Aβ Aggregation and Cytotoxicity in Vitro.-Preparati-on of Cultured Human Vascular Cells. [...]

Fields of interestProtein Science

Target groupsProfessional/practitioner

Discount group P

 

Due January 2012

2nd ed. 2012. X, 650 p. 115 illus., 46 in color. (Methods in Molecular Biology, Volume 849) Hardcover7 $159.00ISBN 978-1-61779-550-3

9   

K. Turksen, Ottawa Hospital Research Institute, ON, Canada (Ed.)

Planar Cell PolarityMethods and Protocols

Contents Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Lo-cations.- Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell.- Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus.- Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity.- Analysis of Cell Shape and Polarity during Zebra-fish Gastrulation.- Analyzing Planar Cell Polarity during Zebrafish Gastrulation.- Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastru-lation.- Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling.- Gene Loss-of-Function and Live Imaging in Chick Embryos.- Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation.- Convergent Extension Ana-lysis in Mouse Whole Embryo Culture.- Analysis of PCP Defects in Mammalian Eye Lens.- Exami-ning Planar Cell Polarity (PCP) in the Mammalian Cochlea.- Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells.- The Planar Cell Polarity Pathway and Parietal Endo-derm Cell Migration.- Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells.- Regulation of Focal Adhesion Dynamics by Wnt5a Signaling.- The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity.- Assessing PCP in the Cochlea of Mammalian Ciliopathy Models.- Morphometric Analysis of Centrosome Position in Tissues.

Fields of interestCell Biology; Cell Physiology

Target groupsProfessional/practitioner

Discount group P

 

Due January 2012

2012. XVIII, 244 p. 59 illus., 27 in color. With online files/update. (Methods in Molecular Biology, Volume 839) Hardcover7 $119.00ISBN 978-1-61779-509-1

9     

G. Witzany, Telos, Bürmoos, Austria; F. Baluška, University of Bonn, Germany (Eds)

Biocommunication of PlantsFeatures 7 This volume documents how plant ecosphe-re inhabitants communicate with each other to coordinate their behavioral patterns and shows the role of viruses in this highly dynamic interactional networks 7 State-of-the-art contentstate-of-the-art contents 7 Gives a modern approach

Contents Keylevels of Biocommunication in Plants.- Infor-mation and Communication in Higher Plants.- Plant Hormones and Metabolites as Universal Vo-cabulary in Pathogen Defense Signaling.- Gravity Sensing/Interpretation and Response Behavior.- Wound Response to Mechanical Damage.- Trans-port and Signaling via the Phloem.- Intercellular Communication During Floral Development.- Cell Wall Signaling.- Plant Root Signaling Between Same, Related and Non-related Plant Roots.- PCD and Plant Life Cycle.- Herbivore- and Pathogen-derived Signals that Induce or Suppress direct and Indirect Defenses in Plants.- Nematode-Plant Communication and Rhizobial-Plant Communi-cation.- Plant Virus Operations Control Centers.- Plant-Microbe Interactions.- Recent Trends on the Olfactory Responses of Insect Natural Enemies to Plant Volatiles.- Plant Defense Against Insect Herbivore Attack.- Volatiles Mediating Infor-mation Between Bacteria and Plants.- Infection of Plants by the Human Pathogen Salmonella typhimurium.- Co-adaptationary Aspects of the Underground Communication Between Plants and Other Organisms.- Mutual Communication of Plants, Animals, Fungi and Bacteria.

Fields of interestPlant Ecology; Plant Sciences; Plant Biochemistry

Target groupsResearch

Discount group P

Available

2012. XV, 373 p. 27 illus., 15 in color. (Signaling and Communication in Plants, Volume 14) Hardcover7 $209.00ISBN 978-3-642-23523-8

9   

www.springer.com/978-1-61779-550-3www.springer.com/978-1-61779-509-1www.springer.com/978-3-642-23523-8