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Supporting Information Near Infrared Dye-Conjugated Oxidative Stress Amplifying Polymer Micelles for Dual Imaging and Synergistic Anticancer Phototherapy Wonseok Yang, Jeongyoun Noh, Hoyeon Park, Sian Gwon, Berwin Singh, Chulgyu Song, Dongwon Lee* 1
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Supporting Information
Near Infrared Dye-Conjugated Oxidative Stress Amplifying Polymer Micelles for Dual Imaging and Synergistic Anticancer Phototherapy
Wonseok Yang, Jeongyoun Noh, Hoyeon Park, Sian Gwon, Berwin Singh, Chulgyu Song, Dongwon Lee*
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Figure S1. A synthetic and degradation route of HRGP-IR.
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Figure S2. 1H NMR spectrum of HRGP recorded in CDCl3.
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(b)
Figure S3. Characterization of HRGP-IR. (a) Gross photographs of HRGP and HRGP-IR. (b)
1H NMR spectrum of HRGP-IR recorded in CDCl3.
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Figure S4. Temperature change of HRGP-IR micelles with different contents of IR820 during NIR laser irradiation. (a) IR820 of 7 wt %. (b) IR820 of 15 wt %. (c) IR820 of 30 wt %. Values are mean±SD (n=4).
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Figure S5. Representative fluorescence images of SW620 cells treated with HRGP-IR micelles.
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Figure S6. Intracellular generation of ROS by HRGP-IR micelles. (A) Effects of the concentration of HRGP-IR micelles on the intracellular ROS generation. (B) Quantitative analysis of ROS generation in cells treated with HRGP micelles. Values are mean±SD (n=4). *p<0.05, ***p<0.001.
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Figure S7. Cytotoxicity of HRGP-IR micelles in the presence of catalase and thiourea. Values are mean±SD (n=4). ***p<0.001 relative to a group of HRGP-IR200 µg/mL.
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Figure S8. Flow cytometric analysis of apoptotic cell death of DU145 cells after co-treatment of HRGP-IR with NIR laser irradiation.
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Figure S9. Blood circulation of HRGP-IR micelles in mice. (a) Fluorescence images of blood containing capillary tubes. (b) Quantified fluorescent intensity of blood containing capillary tubes. Mice were injected with HRGP-IR micelles and blood (20 L) was taken into a capillary tube at given time points. The fluorescence image of blood was made and the fluorescence intensity per unit volume of blood was quantified. Values are meanSD. (n=4).
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NH2
OH
HN NHO
O
O
OO
OOn
O
ON
OH
O
ON N
m
O
O
O
On
O
OO
O
ON
O
O
ON N
m
O
O
O
On
O
OO
N
H3C CH3
SO
OO
N
H3C CH3
SO
OONa
Poly-IR820
Figure S10. A synthetic route and chemical structure of poly-IR820.
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Figure S11. Representative images of TUNEL stained tumor tissues.
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Figure S12. Representative images of H&E stained tissues.
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