Application of Fine Bubbles in Enzyme Catalyzed Multi-Phase Reaction Systems … · 2019. 9....
1
Application of Fine Bubbles in Enzyme Catalyzed Multi-Phase Reaction Systems B. Thomas 1 , D. Ohde 1 , S. Mattes 2 , K. Gupta 1 , C. Engelmann 1 , P. Bubenheim 1 , K. Terasaka 3 , M. Schlüter 2 , A. Liese 1 1 Hamburg University of Technology, Institute of Technical Biocatalysis, Hamburg, Germany 2 Hamburg University of Technology, Institute of Multiphase Flows, Hamburg, Germany 3 Keio University, Department for Applied Chemistry, Tokyo, Japan References: [1] Tsuge, Hideki.. Boca Raton, Florida : CRC Press; Pan Stanford Publishing, (2015). [2] Terasaka, Koichi, et al. obic activated sludge. Chemical Engineering Science. 2011, Bd. 66, 14, S. 3172–3179. [3] Khuntia, Snigdha, Majumder, Subrata Kumar und Ghosh, Pallab.. Reviews in Chemical Engineering. 2012, Bd. 28, 4-6. [4] http://hdwpro.com/free-raspberry-wallpapers.html [5] Wu,et al, 2006.. AIChE J. 52, 2323–2332 [6] Doran, 2013, 2nd ed. Waltham, MA Academic Press Acknowledgement: We are grateful to the Deutsche Forschungsgemeinschaft (DFG) for financial support (DFG, LI 899/10-1) and to all our cooperation partners, especially c-LEcta for the supply of the NADH oxidase. What are Fine Bubbles? • Investigation of mass transport phenomena in gas/liquid reactions is of major importance in chemical and biochemical applications • High demand for new technologies with elevated mass transfer performance [1] • One option to achieve this goal is the aeration with fine bubbles, whose diameters is typically less than 100 micrometers [2,3] • Application of different bubble size classes by three sparger types • Microbubbles: 0.5 μm sinter stone • Submilllibubbles: 10 μm sinter stone • Macrobubbles: 1 x d = 6 mm open pipe Enhancement of Reaction Rate by Fine Bubble Technology Fig. 1: Definition of fine bubbles size classes [1] • Detailed investigation of size and power input on enzymatic rhododendrol oxidation • The aim is to improve the mass transfer and enzyme stability regarding oxygen consuming enzymatic reactions to overcome limitations of conventional aeration systems Mass transfer and enzyme stability is the key! Comparison of Aeration Systems Fig. 2: Raspberry ketone formation in a multiphase system ADH = alcohol dehydrogenase, NOX = NADH oxidase (two phase system with indication of boundary layer, dashed lines) [4] Contact: Benjamin Thomas Institute of Technical Biocatalysis Hamburg University of Technology Denickestr. 15; 21073 Hamburg Office: DE15, 2504 Tel. +49-40-42878-2864 E-mail: [email protected] Highest analytical yield of Y = 89 % after 3h with submilllibubble aeration (10 μm sinter stone) and axial flow regime at low power input of 2.62 W/L Microbubble aeration is independent of power input, shear induced gas dispersion and flow regime Pitch blade turbine induced axial flow regime shows highest efficiency due to low power input, resulting in power number of 1.36 and shear constant 5.4 Fig. 4: Raspberry ketone formation in axial flow regime ( V = 250 mL, c rhododendrol = 10 mM, ѵ ADH-97L = 0.3 U/mL media , ѵ NOX-34 = 0.3 U/mL media , c NAD+ = 0.2 mM, 25 °C, 0.03 vvm, 10 mM Tris-HCl pH = 8) Flow Regime Macrobubble Submillibubble Microbubble [5] [5] Radial Axial Fig. 3: Raspberry ketone formation in radial flow regime ( V = 250 mL, c rhododendrol = 10 mM, A ADH-97L = 0.3 U/mL media , A NOX-34 = 0.3 U/mL media , c NAD+ = 0.2 mM, 25°C, 0.03 vvm, 10 mM Tris-HCl pH = 8) Based on calculation for pure water at 25 °C Based on calculation for pure water at 25 °C
Transcript of Application of Fine Bubbles in Enzyme Catalyzed Multi-Phase Reaction Systems … · 2019. 9....
Application of Fine Bubbles in Enzyme
Catalyzed Multi-Phase Reaction Systems
B. Thomas1, D. Ohde1, S. Mattes2, K. Gupta1, C. Engelmann1, P. Bubenheim1, K. Terasaka3, M. Schlüter2, A. Liese1
1Hamburg University of Technology, Institute of Technical Biocatalysis, Hamburg, Germany2Hamburg University of Technology, Institute of Multiphase Flows, Hamburg, Germany
3Keio University, Department for Applied Chemistry, Tokyo, Japan
References:[1] Tsuge, Hideki.. Boca Raton, Florida : CRC Press; Pan Stanford Publishing,
(2015).
[2] Terasaka, Koichi, et al. obic activated sludge. Chemical Engineering Science.
2011, Bd. 66, 14, S. 3172–3179.
[3] Khuntia, Snigdha, Majumder, Subrata Kumar und Ghosh, Pallab.. Reviews in
Chemical Engineering. 2012, Bd. 28, 4-6.
[4] http://hdwpro.com/free-raspberry-wallpapers.html
[5] Wu,et al, 2006.. AIChE J. 52, 2323–2332
[6] Doran, 2013, 2nd ed. Waltham, MA Academic Press
Acknowledgement:
We are grateful to the Deutsche Forschungsgemeinschaft (DFG) for financial support (DFG, LI
899/10-1) and to all our cooperation partners, especially c-LEcta for the supply of the NADH oxidase.
What are Fine Bubbles?
• Investigation of mass transport phenomena in gas/liquid reactions is of major importance in
chemical and biochemical applications
• High demand for new technologies with elevated mass transfer performance [1]
• One option to achieve this goal is the aeration with fine bubbles, whose diameters is typically less
than 100 micrometers [2,3]
• Application of different bubble size classes by three sparger types
• Microbubbles: 0.5 µm sinter stone
• Submilllibubbles: 10 µm sinter stone
• Macrobubbles: 1 x d = 6 mm open pipe
Enhancement of Reaction Rate by Fine Bubble Technology
Fig. 1: Definition of fine bubbles size classes [1]
• Detailed investigation of size and power input on enzymatic
rhododendrol oxidation
• The aim is to improve the mass transfer and enzyme
stability regarding oxygen consuming enzymatic reactions to
overcome limitations of conventional aeration systems
Mass transfer and enzyme stability is the key!
Comparison of Aeration Systems
Fig. 2: Raspberry ketone formation in a multiphase system ADH = alcohol dehydrogenase, NOX = NADH oxidase (two phase
system with indication of boundary layer, dashed lines) [4]
Contact: Benjamin Thomas
Institute of Technical Biocatalysis
Hamburg University of Technology
Denickestr. 15; 21073 Hamburg
Office: DE15, 2504
Tel. +49-40-42878-2864
E-mail: [email protected]
Highest analytical yield of Y = 89 % after 3h with submilllibubble aeration (10 µm sinter stone) and axial flow regime at low power input of 2.62 W/L
Microbubble aeration is independent of power input, shear induced gas dispersion and flow regime
Pitch blade turbine induced axial flow regime shows highest efficiency due to low power input, resulting in power number of 1.36 and shear constant 5.4
Fig. 4: Raspberry ketone formation in axial flow regime ( V = 250 mL, crhododendrol = 10 mM, ѵADH-97L = 0.3 U/mLmedia, ѵNOX-34 = 0.3 U/mLmedia, cNAD+ = 0.2 mM, 25 °C, 0.03 vvm, 10 mM Tris-HCl pH = 8)
Flow Regime Macrobubble Submillibubble Microbubble
[5]
[5]
Radial
Axial
Fig. 3: Raspberry ketone formation in radial flow regime ( V = 250 mL, crhododendrol = 10 mM, AADH-97L = 0.3 U/mLmedia, ANOX-34 = 0.3 U/mLmedia, cNAD+ = 0.2 mM, 25°C, 0.03 vvm, 10 mM Tris-HCl pH = 8)
Based on calculation for pure water at 25 °C
Based on calculation for pure water at 25 °C
