Application of Endocrine Techniques in Fish Spawning
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Application of Endocrine Techniques in Fish Spawning CONCLUSION Induced spawning of cultured tropical finfishes Background Factors contributing to successful spawning in captivity • Closing the reproductive cycle of farmed species would eliminate the reliance on wild stocks for brooder fish or fingerlings and hence improve the sustainability of commercial aquaculture production. • Hormonal manipulation of reproductive function in cultured fishes is a reliable way to produce fertilized eggs all year round according to hatchery operation plans. Asian Seabass (Lates calcarifer) Sexual maturity Nutrition Health Water quality Photoperiod Tank size Water volume Water flow rate Stocking density Spawning substrate Type of hormone Hormone dosage Hormone preparation and storage Fish handling Fish injection techniques Sterile needles, syringes and saline water Fish-related Environment-related Procedure-related The brain-pituitary-gonad (BPG) axis in fish Asian seabass (aka barramundi) is a tropical marine finfish commonly farmed in Singapore and the SEA region. Suitable biological attributes for aquaculture: Euryhaline (can be farmed in fresh, brackish and marine conditions) Relatively hardy High fecundity (up to 2-3 million eggs per female per spawning) Grow rapidly Flexible harvest size (700 g – 3 kg) Hormone: LHRH-a; Injection method: intra-muscular; Dosage: Male: 20 μg /kgBW; Female: 40 μg /kgBW Broodstock holding tanks Sex checking by cannulation Hormone injection Pompano (Trachinotus blochii) • MAC recorded its 1st successful spawning of pompano in 2011. • Project won Minister’s Team Award 2012. • MAC worked with a local hatchery to transfer the technology and developed a pompano breeding programme for the farm. Hormone: LHRH-a + HCG; Injection method: intra-muscular; Dosage: Male: 187.5 IU hCG/kgBW + 7.5 μg LHRH-a/kgBW Female: 250 IU hCG/kgBW + 10 μg LHRH-a/kgBW Fertilised Egg Day 1 Day 6 Day 13 Day 20 Jade Perch (Scortum barcoo) • Jade perch is a freshwater finfish species native to Australia. • It is hardy and is a fast grower (reaches market size in 7 months). Hormone: Ovaprim; Injection method: Intra-peritoneal; Dosage: 0.5 ml /kgBW for both male and female Male (top) & Female (btm) Cannulation Stripping Cobia (Rachycentron canadum) MAC had assisted a local farm to develop cobia spawning and larviculture protocol. Hormone: LHRH-a; Injection method: intra-muscular; Dosage: Male: 10 μg /kgBW; Female: 15 μg /kgBW Broodstock selection Hormone injection Fertilised eggs Larvae development Spawning and larviculture methods are developed and transferred to local hatcheries Mylonas et al., 2009 Aquaculture Department, Urban Food Solutions, SFA
Transcript of Application of Endocrine Techniques in Fish Spawning
Application of Endocrine Techniques in Fish Spawning
CONCLUSION
Induced spawning of cultured tropical finfishes
Background
Factors contributing to successful spawning in captivity
• Closing the reproductive cycle of farmed species would eliminate the reliance on wild stocks for brooder fish or fingerlings and hence improve the sustainability of commercial aquaculture production.
• Hormonal manipulation of reproductive function in cultured fishes is a reliable way to produce fertilized eggs all year round according to hatchery operation plans.
Asian Seabass (Lates calcarifer)
Sexual maturity
Nutrition
Health
Water quality
Photoperiod
Tank size
Water volume
Water flow rate
Stocking density
Spawning substrate
Type of hormone
Hormone dosage
Hormone preparation and storage
Fish handling
Fish injection techniques
Sterile needles, syringes and saline water
Fish-related Environment-related Procedure-related
The brain-pituitary-gonad (BPG) axis in fish
Asian seabass (aka barramundi) is a tropical marine finfish commonlyfarmed in Singapore and the SEA region.Suitable biological attributes for aquaculture: Euryhaline (can be farmed in fresh, brackish and marine conditions) Relatively hardy High fecundity (up to 2-3 million eggs per female per spawning) Grow rapidly Flexible harvest size (700 g – 3 kg)
Hormone: LHRH-a; Injection method: intra-muscular;Dosage: Male: 20 µg /kgBW; Female: 40 µg /kgBW
Broodstock holding tanks Sex checking by cannulation Hormone injection
Pompano (Trachinotus blochii)
• MAC recorded its 1st successful spawning of pompano in 2011.• Project won Minister’s Team Award 2012.• MAC worked with a local hatchery to transfer the technology and
developed a pompano breeding programme for the farm.Hormone: LHRH-a + HCG; Injection method: intra-muscular;Dosage: Male: 187.5 IU hCG/kgBW + 7.5 µg LHRH-a/kgBWFemale: 250 IU hCG/kgBW + 10 µg LHRH-a/kgBW
Fertilised Egg Day 1 Day 6 Day 13 Day 20
Jade Perch (Scortum barcoo)
• Jade perch is a freshwater finfish species native to Australia.• It is hardy and is a fast grower (reaches market size in 7 months).Hormone: Ovaprim; Injection method: Intra-peritoneal;Dosage: 0.5 ml /kgBW for both male and female
Male (top) & Female (btm) Cannulation Stripping
Cobia (Rachycentron canadum)
MAC had assisted a local farm to develop cobia spawning and larvicultureprotocol.Hormone: LHRH-a; Injection method: intra-muscular;Dosage: Male: 10 µg /kgBW; Female: 15 µg /kgBW
Broodstock selection Hormone injection Fertilised eggs Larvae development
Spawning and larviculture methods are developed and transferred to local hatcheries
Mylonas et al., 2009
Aquaculture Department, Urban Food Solutions, SFA
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