Apoptosis Catalog - R&D Systems

R&D Systems Apoptosis Catalog

USA & Canada R&D Systems, Inc. 614 McKinley Place NE

Minneapolis, MN 55413, USATEL: (800) 343-7475 (612) 379-2956

FAX: (612) 656-4400E-MAIL: [email protected]

www.RnDSystems.com

UK & Europe R&D Systems Europe Ltd. 19 Barton Lane, Abingdon Science Park

Abingdon OX14 3NB, UKTEL: +44 (0)1235 529449FAX: +44 (0)1235 533420

E-MAIL: [email protected]. RnDSystems.com

China R&D Systems China Co., Ltd. 25/G Hua Min Empire Plaza

726 West Yan An RoadTEL: (21) 52380373FAX: (21) 52371001

E-MAIL: [email protected]

International Distributors Please visit www.RnDSystems.com for a full list of international distributors.

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Tools for Cell Biology Research™

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Apoptosis Detection Kits

Apoptosis Detection KitsCell Viability & Proliferation Assays ........................................................................3-4

• TACS™ MTT ..........................................................................................................3

• TACS XTT .............................................................................................................3

• Calcein AM ..........................................................................................................4

• Resazurin .............................................................................................................4

Annexin V Assays ..............................................................................................................5

• TACS Annexin V-Biotin in situ ........................................................................5

• TACS Annexin V-Fluorescein in situ ............................................................5

DePsipher™ Mitochondrial Membrane Potential Assay .....................................6

ApoStat Apoptosis Detection Kit ................................................................................6

Caspase Colorimetric and Fluorometric Activity Assays ....................................7

PARP Activity Assay ..........................................................................................................7

Single Cell Gel Electrophoresis Assay/CometAssay™ ..........................................8

• CometAssay-Silver ............................................................................................8

• CometAssay-SYBR® ..........................................................................................8

TACS DNA Laddering Detection Kits ........................................................................9

• Ethidium Bromide Assay ................................................................................9

• Isotopic Assay.....................................................................................................9

• Chemiluminescent Assay ...............................................................................9

• Colorimetric Assay ............................................................................................9

TUNEL Labeling Kits ............................................................................................... 10-13

Light & Electron Microscope-based Kits .................................................. 10-11

Fluorescence & Flow Cytometry-based Kits ..................................................11

Microplate Reader-based Kits ..............................................................................12

Tissue type-specifi c Colorimetric Kits ...............................................................12

• CardioTACS™ .....................................................................................................12

• DermaTACS™ ....................................................................................................12

• NeuroTACS™......................................................................................................13

• TumorTACS™ .....................................................................................................13

• VasoTACS™ .........................................................................................................13

Supplemental Apoptosis Reagents .........................................................................14

Proteome Profi ler™ Apoptosis Array Kit ............................................................ 15-16

Apoptosis-related Molecule FamiliesCaspases & Regulators ........................................................................................... 18-23

Inhibitors of Apoptosis & Regulators ............................................................... 24-27

Extrinsic Pathway of Caspase Activation ........................................................ 28-62

• TNF Superfamily ....................................................................................... 28-57

• Apoptosis Adaptor Proteins ................................................................. 58-62

Intrinsic Pathway of Caspase Activation ......................................................... 63-82

• Bcl-2 Family ................................................................................................ 63-69

• Mitochondrial Proteins .......................................................................... 70-72

• Oxidative Stress ........................................................................................ 73-78

• Heat Shock Proteins ................................................................................ 79-82

Regulation of Pro- and Anti-Apoptotic Signaling Pathways ...............................................................................................83-110

• Intracellular Kinases ................................................................................ 83-96

• NFκB ...........................................................................................................97-100

• p53 Family ............................................................................................. 101-104

• Other Transcription Factors ............................................................. 105-110

Granzyme-mediated Cell Death ................................................................... 111-114

• Cytolytic Granule Proteins ............................................................... 111-114

Other Apoptosis Molecules ............................................................................ 115-117

Index............................................................................................................. 118-120

IntroductionStrategies for Apoptosis Detection ............................................................................2

U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373

2 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373

Apoptosis, also called programmed cell death, is a coordinated and step-wise series of biochemical reactions resulting in the ordered disassembly and removal of a cell from an organism. Initiation of apoptosis leads to the activation of intracellular proteases, endonucleases, and other enzymes which proteolytically dismantle the cell by cleaving key target substrates. These intracellular events lead to signifi cant cellular changes characteristic of apoptosis, including cytoplasmic condensation and cell shrinkage, chromatin condensation, membrane blebbing, nuclear fragmentation, and the formation of apoptotic bodies which are cleared by phago-cytes. Apoptosis is a widespread biological process needed for proper organ development during embryogenesis and removal of cells damaged by exposure to pathogens or events associated with oncogenic transformation. When apoptosis occurs on schedule, neighboring cells and the organism itself are not adversely affected. Errors in apoptosis, however, such as mistiming or resistance, can have dire effects on an organism. For example, if unscheduled apoptosis occurs in irreplaceable cells, functions critical to the organism will be lost. This is the case in some

neurodegenerative disorders. In contrast, cells that resist apoptosis may cause developmental abnormalities or unregulated cell growth. As is the case in some cancers, cells may contain and accumulate mutations but will continue to undergo DNA replication and divide due to defects in apoptotic signaling pathways. Therefore, apoptotic fi delity is critical to the develop-ment and well-being of an organism.

Several cellular changes occur during apoptosis that allow for its detection, including changes in the phospholipid content of the outer leafl et of the plasma membrane, disruption of the electrochemical gradient across the mitochondrial membrane, activation of intracellular caspases, cleavage of Poly(ADP-ribose) Polymerase, DNA fragmentation, and activation of apoptotic signaling cascades. R&D Systems offers an extensive range of kits and reagents to optimize the detection of apoptosis in a variety of different research models. The most appropriate strategy will ultimately depend on the cell or tissue type being analyzed and the method used for inducing apoptosis.

Introduction

PROLIFERATION & VIABILITY ASSAYS

EARLY SIGNS OF APOPTOSIS

LATE EVENTS OF APOPTOSIS

DNA Fragmentation PARP Cleavage

Cell Surface Changes Disruption of Mitochondrial Membrane Potential Caspase Activation

TACS DNA Laddering Kits(pg. 9)

Resazurin(pg. 4)

Calcein AM(pg. 4)

TACS XTT(pg. 3)

TACS MTT(pg. 3)

Colorimetric/Fluorometric Assay

(pg. 7)

ApoStat(pg. 6)

PARP Activity Assay(pg. 7)

DePsipher Kit(pg. 6)

Annexin V(pg. 5)

General Kits

TACS TdT(pg. 10)

Specific Kits

TUNEL Assays(pg. 10-13)

Flow Cytometry

FlowTACS(pg. 11)

High throughput Screening

TiterTACS(pg. 12)

Tissue or Cell-typeSpecific Applications

CardioTACS

DermaTACS

NeuroTACS

TumorTACS

VasoTACS(pgs. 12-13)

FluorescenceLight

CometAssay(pg. 8)

CometAssay-Silver(pg. 8)

CometAssay-SYBR(pg. 8)

Interested in Apoptotic Signaling Pathways?

R&D Systems Proteome Profiler™ Apoptosis Array (page 15),Antibodies, Proteins, & ELISA Kits (see product listings in the Apoptosis-Related Molecule Families section of this catalog).

Strategies for Apoptosis Detection

R&D Systems Tools for Cell Biology Research™


3For research use only. Not for use in diagnostic procedures. www.RnDSystems.com

Cell Viability and Proliferation AssaysCell viability and proliferation assays can be used to evaluate whether apoptosis may be occurring in an experimental system. Cell viability refers to the number of healthy cells in a sample, while proliferation is the number of dividing cells. Cells undergoing apoptosis demonstrate a decrease in both cell proliferation and viability. While counting cells directly using a hemacytometer may be the most straightforward approach, there are other more rapid, quantitative, and accurate assays and reagents available for measuring cell viability and proliferation. Since proliferating cells are metabolically more active than quiescent cells, the following assays are suitable fordetermining proliferation, as well as cell viability and cytotoxicity.

TACS™ MTT AssayCells undergoing proliferation or apoptosis demonstrate an increase or decrease, respectively, in their ability to reduce the tetrazolium salt, MTT. In metabolically active cells, MTT is converted to insoluble purple formazan dye crystals. Addition of detergent solubilizes the crystals, which can then be measured by absorbance using a spectrophotometer. This microplate-based assay allows for the safe, sensitivemeasurement of cell proliferation and viability and is ideal for high throughput platforms. The rate of tetrazolium reduction is proportional to the rate of proliferation.

KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE

Colorimetric Assay For the measurement of cell proliferation based on the reduction of the tetrazolium salt, MTT. Cells undergoing apoptosis have a decreased ability to reduce MTT.

3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)

• Microplate reader • Unfi xed cells TA5355TA5412

2500 Tests5000 Tests

TACS™ XTT AssayXTT is a modifi ed tetrazolium salt that is cleaved to form a soluble orange formazan dye in metabolically active cells. This dye can be directly measured by absorbance without an additional solubilization step. This microplate-based assay allows for the safe, sensitivemeasurement of cell proliferation and viability and is ideal for high throughput platforms. The rate of tetrazolium reduction is proportional to the rate of proliferation.

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1.0

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Corre

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Abs

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A490

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630 n

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250

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50 75 100 125

Seeding Density (Cells/mL x 104)

Relative Cell Number Measured with XTTMurine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densi-ties. Cells were incubated with XTT (Catalog # 4891-025-K) for 6 hours at 37° C and absorbances were read using an ELISA plate reader.


Colorimetric Assay For the measurement of cell proliferation based on the reduction of the tetrazolium salt, XTT. Cells undergoing apoptosis have a decreased ability to reduce XTT.

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)

• Microplate reader • Unfi xed cells 4891-025-K 2500 Tests




Calcein AMCalcein AM is a non-fl uorescent, hydrophobic compound that easily permeates intact, living cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fl uorescent compound that is retained in the cytoplasm of cells with intact membranes. Measuring fl uorescence provides an indication of viable cell number. Calcein AM is suitable for use with both cells in suspension and adherent cells to measure cell viability. It may be utilized in conjunction with fl ow cytometry, immunocytochemistry, and in vivo cell tracing techniques.

KIT SPECIES TESTING FORMAT SAMPLE TYPE CATALOG # SIZE

Calcein AM Cell Viability Assay Multi-species • Microplate reader • Unfi xed cells 4892-010-K 1000 Tests

Resazurin Resazurin, also known as AlamarBlue®, is a non-toxic, water soluble, redox-sensitive dye that changes from its blue/non-fl uorescent state to a pink/highly-fl uorescent state upon reduction to resorufi n in viable cells. Resazurin may be added to cells at any time during the culture period, but for proliferation assays, it is optimal to add it during log-phase growth. Incubation times may vary depending on the metabolic rates of the cells being tested. Resazurin can be utilized as a simple and quantitative method for measuring cell proliferation, viability, and cytotoxicity.

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Measurement of Cell Proliferation using ResazurinCCR2A-transfected murine Baf-3 cells were grown in the presence of Resazurin (Catalog # AR002). Resorufi n fl uorescence was read in a fl uorescent plate reader.

KIT DESCRIPTION CATALOG # SIZE

Resazurin For use with Cell Proliferation/Viability Assays AR002 100 mL



Annexin V AssaysEarly in the apoptotic process, the characteristic cell surface phospholipid asymmetry is disrupted. This leads to the exposure of phosphatidylserine (PS) on the outer leafl et of the cell membrane. Annexin V is an anticoagulant protein that preferentially binds PS and, when conjugated to a reporter molecule, can be used as an indicator of apoptosis. The Annexin V conjugate may be used in fl ow cytometry or in other in situ detection methods to facilitate the rapid and early detection of cell surface changes associated with apoptosis. Annexin V binding to PS occurs only in dying cells, is calcium-dependent, and reversible. Each Annexin V molecule binds approximately 50 exposed PS monomers.

Annexin V

Induction of Apoptosis

Annexin V Binding

Plasma Membrane

phosphatidylserine

phosphatidylserinePhospholipid Flipping

Normal Cell Apoptotic Cell

Ca2+Ca2+

Ca2+

Schematic Representation of Apoptosis-induced Membrane Changes Recognized by Annexin VReorganization of the plasma membrane occurs early in the apoptotic process disrupting phospholipid asymmetry and leading to the exposure of phosphatidylserine on the outer leafl et of the cytoplasmic membrane. In the presence of calcium, Annexin V binds to phosphatidylserine allowing apoptotic cells to be easily identifi ed by fl ow cytometry or fl uorescence microscopy.

ViableViable

EarlyApoptotic

Late Apoptotic

EarlyApoptotic

Late Apoptotic

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Annexin V-FITC

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Annexin V-FITC

Detection of Apoptotic Dexamethasone-treated Thymocytes by Annexin V StainingThymocytes were left untreated (A) or treated with 100 nM dexamethasone for 15.5 hours (B) and then stained using Annexin V-FITC and propidium iodide provided in the Annexin V-FITC Apoptosis Detection Kit (Catalog # TA4638). The combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Analysis courtesy of Dr. C.M. Knud-son, Howard Hughes Medical Institute, St. Louis, MO.

KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE

TACS Annexin V - Biotin in situ Streptavidin-Fluorochrome Conjugate (not provided)

• Flow Cytometry• Fluorescence microscopy

• Fixed Cells* TA4619 100 Tests

TACS Annexin V - Fluorescein in situ Fluorescein • Flow Cytometry• Fluorescence microscopy

• Unfi xed cells TA4638TA5532

100 Tests250 Tests

*Cells are fi xed after labeling with Annexin V-Biotin.

BA



DePsipher™ Mitochondrial Membrane Potential AssayDisruption of the electrochemical gradient across the mitochondrial membrane is one of the fi rst intracellular changes to occur following the onset of apoptosis. The DePsipher Assay uses a lipophilic cation to identify changes in the potential of the mitochondrial membrane.In normal, healthy cells, the DePsipher reagent aggregates in the mitochondria to form an orange fl uorescent compound. In apoptotic cells, when the mitochondrial membrane potential is disrupted, the DePsipher reagent remains in its monomeric form and fl uoresces green. These changes in fl uorescence can be monitored by either microscopy or fl ow cytometry. The DePsipher Assay Kit is useful for evaluating cellular viability, estimating the effect of drugs or other cytotoxic treatments on cells, and detecting early apoptosis in known models.

Identifi cation of Apoptotic INT407 Cells using the DePsipher KitINT407 human embryonic intestinal cells were treated with 25µM etoposide for 8 hours and then incubated with the DePsipher reagent in reaction buffer for 30 minutes (both provided in the DePsipher Kit, Catalog # TA700). Healthy cells (containing red aggregates) were differentiated from apoptotic cells (containing only green monomers, indicated by the blue arrow) by fl uorescence microscopy.


DePsipher™ Assay Lipophilic cation • Flow Cytometry• Fluorescence Microscopy

• Unfi xed Cells TA700 100 Tests

ApoStat Apoptosis Detection KitApoStat is designed to identify and quantitate caspase activity in apoptotic cells by fl ow cytometry. Cells undergoing apoptosis are irreversibly labeled with a cell permeable, FITC-conjugated pan-caspase inhibitor (ApoStat). Any unbound reagent diffuses out of the cell and is washed away. Cells are then analyzed by fl ow cytometry for the presence of bound reagent. Increased fl uorescence is an indicator of caspase activity within cells. The detection of active caspases by fl ow cytometry is a rapid method for identifying cells undergoing apoptosis.

47.8% Positive Events

10.2% Positive Events

Positive Staining Region

Rela

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101100 102 103 104

ApoStat Staining

Detection of Caspase Activation in Jurkat CellsHuman Jurkat leukemic T cells were cultured in the absence (green histogram) or presence (purple histogram) of 1 µM staurosporine for 3.5 hours and then stained with ApoStat (Catalog # FMK012) for 30 minutes. The cells were harvested, washed, and assayed by fl ow cytometry to determine the percentage of cells expressing active caspases.


ApoStat Assay FITC-VD-FMK FITC • Flow Cytometry • Cultured cells FMK012 100 Tests



Caspase Colorimetric & Fluorometric Activity AssaysThe Caspase Colorimetric and Fluorometric Activity Assays* can be used to determine the enzymatic activity of specifi c caspases in apoptotic cells. Cell lysates are tested for protease activity by the addition of a caspase-specifi c peptide substrate that is conjugated to a colorimetric or a fl uorescent reporter molecule. Cleavage of the peptide by a specifi c caspase releases the chromophore or the fl uorochrome which can subsequently be measured spectrophotometrically. The fl uorochrome can be excited to emit fl uorescence at a specifi c wavelength and detected by a fl uorimeter or a fl uorescence microplate reader. The colorimetric or fl uorometric signal detected in these assays is directly proportional to the enzymatic activity of the caspase. *Please refer to the product listing under the Caspases & Regulators section of this catalog for colorimetric and fl uorometic activity assays available for individual caspases.

PARP Activity AssayPoly (ADP-ribose) Polymerase (PARP) is an abundant enzyme present in all somatic cells that is activated upon DNA damage. It attaches to regions of damaged DNA and catalyzes the NAD-dependent ADP-ribosylation of itself and adjacent nuclear proteins. Poly ADP-ribosylation plays a role in regulating the activities of proteins involved in DNA repair, genomic stability, and cell cycle arrest. During apoptosis, PARP is specifi cally cleaved by caspases and is no longer activated by DNA damage, preventing apoptotic cells from repairing their DNA. The PARP Universal Colorimetric Assay measures the activity of PARP in cells and tissues by detecting the incorporation of biotinylated poly(ADP-ribose) onto histone proteins. This assay is useful for testing whether damaged DNA is from non-apoptotic cells or to assay the effectiveness of PARP inhibitors.

Histones

PARP Cleavage ADP-ribosylation

DNA Damage

Sites of damage

poly(ADP-ribose)

Apoptosis DNA Repair

Repaired DNADNA Fragmentation

PARP

PARP

PARP PARPPARP

PARPPARP

PARP

PARPPARP

PARPPARP

X

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PAR P PAR P

PAR P

Schematic Representation of the Function of PARP Following DNA DamageFollowing DNA damage, PARP catalyzes the ADP-ribosylation of itself and adjacent nuclear proteins. This modifi cation is involved in signaling DNA damage and aiding in DNA repair in normal, viable cells (right). During apoptosis, PARP is cleaved by caspases and can no longer be activated by DNA damage, preventing apoptotic cells from repairing their DNA (left).


PARP Universal Colorimetric Assay

TACS-Sapphire™ • Colorimetric Assay • Unfi xed cells• Fresh tissues

4677-096-K 96 Tests



Single Cell Gel Electrophoresis Assay/CometAssay™The CometAssay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage. The principle of the assay is based upon the ability of denatured cleaved DNA fragments to migrate out of the cell under the infl uence of an electric potential (the “comet tail”), whereas undamaged supercoiled DNA remains within the confi nes of the cell membrane (the “comet head”) when a current is applied. The method involves the immobilization of cells in a bed of low melting point agarose, followed by gentle cell lysis. After treatment with alkali to denature the DNA and hydrolyze sites of damage, the samples are submitted to electrophoresis. Samples are subsequently stained with a fl uorescent DNA-intercalating dye or silver stain and visualized using the appropriate microscope.

CometAssay-Silver

Visualization of DNA Damage using the CometAssay and Silver StainingMurine WEHI 7.1 lymphoma cells were treated with potassium permanganate and processed using the CometAssay – Silver Staining Kit (Catalog # TA4251) under alkali conditions. Following electrophoresis,samples were fi xed in cold ethanol and air dried. After drying, the slides were stained as recommended in the kit instructions and analyzed by microscopy. DNA damage is indicated by a “comet tail” shape and the overall migration pattern of the DNA.


CometAssay – Silver Silver • Light microscopy • Unfi xed cells• Fresh tissues

TA4251 50 Tests

CometAssay-SYBR®

Visualization of DNA Damage using the CometAssay – SYBR KitMurine WEHI 7.1 lymphoma cells were treated with potassium permanganate and DNA fragmentation was visualized using the CometAssay – SYBR Kit (Catalog # TA800) under alkali conditions. Following electro-phoresis the samples were fi xed and dried. The slides were subsequently stained following the instructions provided in the kit, and the cells were analyzed by microscopy. Undamaged DNA does not migrate far from the origin, while damaged DNA appears as a fl uorescent “comet tail”.


CometAssay – SYBR® SYBR Green I • Fluorescence microscopy • Unfi xed cells• Fresh tissues

TA800 50 Tests



TACS™ DNA Laddering Detection KitsDNA laddering is a hallmark of apoptotic cells. It is caused by internucleosomal cleavage of the DNA by an endonuclease called Caspase activated DNase (CAD) or DNA Fragmentation Factor 40 (DFF40) which is activated during the later stages of apoptosis. Cleavage of the DNA by CAD/DFF40 forms a characteristic ladder of DNA monomers (180-200 bp) and multimers. R&D Systems offers four different DNA Laddering Kits that allow DNA fragmentation to be detected by agarose gel electrophoresis. DNA is visualized by either luminescent or colorimetric-based assays, by ethidium bromide staining, or by isotope-labeling. Kit selection depends on the number of cells available and the percentage of cells that are apoptotic.

Label & Sample Requirements

Untreated IR treated

Detection of DNA Laddering by Isotopic Labeling of DNA from IR-treated ML-1 CellsDNA from human ML-1 myeloblastic leukemia cells that were either untreated or treated with ionizing radiation (20 Gy) and allowed to recover 48 hours, was isotopically labeled according to the protocol in the TACS DNA Laddering Detection Kit (Catalog # TA4631), and then separated by agarose gel electrophoresis. The gel was subsequently fi xed, dried, and exposed to fi lm for 2 hours. DNA laddering in the cells that were treated with IR is indicative of apoptosis.


Ethidium Bromide Assay Ethidium bromide (not provided) • Gel electrophoresis • Unfi xed cells• Fresh tissues

TA4630 20 Tests

Isotopic Assay [α-32P]-label (not provided) • Gel electrophoresis • Unfi xed cells• Fresh tissues

TA4631 20 Tests

Chemiluminescent Assay PeroxyGlow™ • Gel electrophoresis • Unfi xed cells• Fresh tissues

TA4632 20 Tests

Colorimetric Assay Blue Membrane Solution • Gel electrophoresis • Unfi xed cells• Fresh tissues

TA4633 20 Tests

Ethidium Bromide

Minimum Cell Number 1 x 106

Sensitivity 20% apoptotic

Colorimetric Assay



Chemiluminescent Assay



Isotopic Assay





TUNEL Labeling KitsApoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). R&D Systems offers kits designed for several different formats including light and fl uorescence microscopy, fl ow cytometry, and colorimetric microplate reader-based assays. We also offer colorimetric kits optimized for specifi c tissue types including cardiac, skin, neuronal, tumor, and vascular cells and tissues. For more detailed information, please see our website at www.RnDSystems.com/go/TUNEL.

Light & Electron Microscope-based Kits

Detection of Apoptosis in Post-weaning Mouse Breast Tissue using TACS™ TdT DAB KitPost-weaning mouse breast tissue was fi xed in 10% neutral buffered formalin, embedded in paraffi n, and sec-tioned at fi ve microns. Deparaffi nized and rehydrated sections were subsequently processed using the TACS TdT DAB Kit protocol (Catalog # TA4625) and visualized by light microscopy. Apoptotic nuclei stain brown (arrows) using this protocol.

DNA Fragmentation in Mouse Lung Parenchyma Tissue Detected using the TACS•XL® Blue KitMouse lung parenchyma tissue was fi xed and embedded in paraffi n. Five micron sections were prepared and immobilized on slides. The tissue was labeled as described in the TACS•XL protocol (Catalog # TA300) and ana-lyzed by light microscopy for DNA fragmentation indicated by the blue stained cells (arrows). Data was provided courtesy of F. D’Agostini, University of Genova Via A. Pastore, Genova, Italy.


TACS TdTIn situ Apoptosis Detection Kit - Core Reagents

Provides the core reagents necessary for the TUNEL reaction. Includes 10X TdT Labeling Buffer, TdT-dNTP mix, TdT Enzyme, 10X TdT Stop Buffer, Streptavidin-HRP, 50X Co2+, 50X Mg2+, and 50X Mn2+.

Biotinylated Nucleotides and Streptavidin-HRP. User supplied label

• Light microscopy• Double labeling• Electron

microscopy

• Fixed cells• Frozen tissues• Paraffi n-embedded cells and

tissues• Resin-embedded cells and

tissues

TA4628 30 Tests

TACS TdTIn situ Apoptosis Detection Kit - DAB

A complete kit supplied with Streptavidin-HRP, DAB, Methyl Green counterstain, and reagents necessary for generating positive controls.

Biotinylated Nucleotides & Streptavidin-HRP/DAB


microscopy



tissues

TA4625 30 Tests

TACS TdTIn situ Apoptosis Detection Kit - Blue Label

A complete kit supplied with Streptavidin-HRP, TACS BlueLabel™, Nuclear Fast Red counterstain, and reagents necessary for generating positive controls.

Biotinylated Nucleotides & Streptavidin-HRP/TACS BlueLabel.


microscopy



tissues

TA4626 30 Tests

TACS TdTIn situ Apoptosis Detection Kit - Replenisher

Provides limiting core reagents necessary for the TUNEL reaction. Includes Proteinase K Solution, TdT-dNTP Mix, TdT Enzyme, Streptavidin-HRP, 50X Co2+, 50X Mg2+, and 50X Mn2+.

Biotinylated Nucleotides & Streptavidin-HRP


microscopy



tissues

TA4629 30 Tests



Light & Electron Microscope-based Kits continued


TACS•XLIn situ Apoptosis Detection Kit - Basic

Provides the core reagents necessary for the TUNEL reaction. Includes Cytonin, Proteinase K Solution, 10X TdT Labeling Buffer, 10X TdT Stop Buffer, B-dNTP Mix, TdT Enzyme, Streptavidin-HRP, Streptavidin Diluent, and Anti-BrdU antibody.

Brominated Nucleotides, Biotinylated anti-BrdU, Streptavidin-HRP. User supplied label


microscopy



tissues

TA100 30 Tests

TACS•XL In situ Apoptosis Detection Kit - DAB

A complete kit supplied with biotin-conjugated anti-BrdU, Streptavidin-HRP, DAB, Methyl Green counterstain, and reagents necessary for generating positive controls.

Brominated Nucleotides, Biotinylated anti-BrdU antibody, Streptavidin-HRP/DAB


microscopy



tissues

TA200 30 Tests

TACS•XL In situ Apoptosis Detection Kit - Blue Label

A complete kit supplied with biotin conjugated anti-BrdU, Streptavidin-HRP, TACS Blue Label, Nuclear Fast Red counterstain, and reagents necessary for generating positive controls.

Brominated Nucleotides, Biotinylated anti-BrdU antibody, Streptavidin-HRP/TACS BlueLabel.


microscopy



tissues

TA300 30 Tests

TACS•XL In situ Apoptosis Detection Kit - Replinisher

Provides the limiting, core reagents necessary for the TUNEL reaction. Includes Proteinase K Solution, B-dNTP Mix, TdT Enzyme, Streptavidin-HRP, Streptavidin Diluent, and Anti-BrdU antibody.

Brominated Nucleotides, Biotinylated anti-BrdU antibody, Streptavidin-HRP.


microscopy



tissues

TA400 30 Tests

Fluorescence & Flow Cytometry-based Kits

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Analysis of DNA Fragmentation in Cyclohexamide- or Dexamethasone-treated Murine ThymocytesMurine thymocytes were treated with 10 µg/mL cyclohexamide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled accordingto the FlowTACS protocol (Catalog # TA5354), and analyzed by fl ow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.


TACS TdT In situ Apoptosis Detection Kit - Fluorescein

A complete kit supplied with Streptavidin-Fluorescein and all the reagents necessary for generating positive controls.

Biotinylated Nucleotides and Streptavidin-Fluorescein

• Fluorescence microscopy

• Double labeling• Electron microscopy



tissues

TA4627 30 Tests

FlowTACS™ In situ Apoptosis Detection Kit

A complete kit that comes with all the necessary reagents required for labeling samples and generating positive controls for analysis in Flow Cytometry.

Biotinylated Nucleotides and Streptavidin-Fluorescein

• Flow Cytometry• Fluorescence

microscopy• Double labeling

• Fixed cells TA5354 60 Tests

A B



Microplate Reader-based Kits

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0.2

Detection of Apoptosis in Staurosporine-treated ML-1 CellsHuman ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fi xed and labeled according to the protocol in the TiterTACS Kit (Catalog # TA600) prior to colorimetric analysis. The reac-tion was stopped with 2N HCl.


TiterTACS™ In situ Apoptosis Detection Kit

A complete kit supplied with TACS-Sapphire optimized for colorimetric detection in 96-well plates, and all the reagents necessary for generating positive controls.

Biotinylated Nucleotides and Streptavidin-HRP/TACS Sapphire

• Microplate reader • Suspension or monolayer cell cultures

TA600 96 Tests

Tissue Type-Specifi c Colorimetric Kits

CardioTACS™

Visualization of an Apoptotic Rat Cardiac Myocyte using the CardioTACS In situ Detection KitRat heart tissue was fi xed in 4% formaldehyde overnight and paraffi n-embedded. Five micron sections were prepared and placed onto glass microscope slides. Samples were processed following the CardioTACS Kit protocol (Catalog # TA5353) and visualized by light microscopy. An apoptotic cardiac myocyte is indicated by the arrow. Photo was provided courtesy of Dr. J. Zhang, FDA.


CardioTACS™ In situ Apoptosis Detection Kit

A complete kit supplied with TACS Blue Label and Nuclear Fast Red counterstain. Optimized for labeling samples and generating positive controls in cardiac tissues.

Biotinylated Nucleotides and Streptavidin-HRP/TACS BlueLabel

• Light microscopy• Double labeling• Electron microscopy

• Fixed cells• Frozen tissues• Paraffi n-embedded cells

and tissues• Resin-embedded cells and

tissues

TA5353 30 Tests

DermaTACS™

A

B

C

Detection of DNA Fragmentation in the UVB-irradiated Human Skin Model, EpiDerm™ using the DermaTACS In situDetection KitSkin tissue samples, untreated (A), treated with 180 mJ/cm2 irradiation (B), or with TACS-Nuclease (C), were fi xed in 10% buffered formalin one hour following treatment. Fixed samples were embedded in paraffi n and six micron sections were labeled for DNA fragmentation using the DermaTACS Kit protocol (Catalog # TA4829). The dark blue stained cells exhibit the punctuate morphology indicative of apoptosis (B; arrow). The TACS-Nuclease treated sample shows the diffuse blue staining of fragmented, uncondensed DNA (C; arrows). Samples were provided courtesy of Dr. Patrick Hayden, MatTek Corporation.


DermaTACS™ In situ Apoptosis Detection Kit

A complete kit supplied with TACS Blue Label and Red Counterstain C. Optimized for labeling samples and generating positive controls in skin tissues.

Brominated Nucleotides and Biotinylated anti-BrdU, Streptavidin-HRP/TACS BlueLabel




tissues

TA4829 30 Tests



NeuroTACS™

Neonatal Mouse Cortex Labeled for Apoptosis using the NeuroTACS In situ Apoptosis Detection KitThe cortex of a neonatal mouse, damaged by a two hour hypoxic-ischemic insult to the forebrain, was labeled 24 hours later for in situ apoptosis (brown stained nuclei; arrows) using the NeuroTACS II Kit protocol (Catalog # TA900) and for GFAP using TACS Red label.


NeuroTACS™ II In situ Apoptosis Detection Kit

A complete kit supplied with DAB and Blue counterstain. Optimized for labeling samples and generating positive controls in neuronal samples.

Biotinylated Nucleotides and Streptavidin-HRP/DAB


• Paraffi n-embedded cells and tissues

• Resin-embedded cells and tissues

TA900 30 Tests

TumorTACS™

Apoptotic Cells within a Mouse Mammary Tumor Identifi ed using the TumorTACS KitMammary tumor was fi xed in 4% paraformaldehyde overnight and paraffi n-embedded. Five micron sections were pre-pared and placed onto glass microscope slides. The sample was processed following the TumorTACS Kit protocol (Cata-log # TA5411). Brown stained nuclei indicate apoptotic cells (arrows).


TumorTACS™ In situ Apoptosis Detection Kit

A complete kit supplied with DAB and Methyl Green counterstain. Optimized for labeling samples and generating positive controls in tumor samples.

Biotinylated Nucleotides and Streptavidin-HRP/DAB




tissues

TA5411 30 Tests

VasoTACS™

Visualization of Apoptotic Cells in Vascular TissueTissue from the small artery of a rat was formalin-fi xed and paraffi n-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (Catalog # 4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicate apoptotic cells (arrows). Data was provided courtesy of Dr. Jun Zhang, FDA.


VasoTACS™ In situ Apoptosis Detection Kit

A complete kit supplied with TACS Blue Label and Red Counterstain C. Optimized for labeling samples and generating positive controls in vascular samples.

Biotinylated Nucleotides and Streptavidin-HRP/TACS BlueLabel




tissues

4826-30-K 30 Tests



Supplemental Apoptosis ReagentsREAGENT DESCRIPTION CATALOG # SIZE

4-Amino-1,8-Naphthalimide Inhibitor of poly(ADP-ribose) polymerase (PARP). Can be used with the PARP Assay. 4667-50-09 100 µL

6(5H)-Phenanthridinone Inhibits poly(ADP-ribose) polymerase (PARP) and displays immunosuppressive activity. Can be used with the PARP Assay.

4667-50-10 100 µL

6-Biotin-17-NAD Non-isotopic alternative to radiolabeled NAD for studies requiring this substrate. Can beused with the PARP Assay.

4670-500-01 500 µL

Benzamide Inhibits poly(ADP-ribose) polymerase (PARP). It is able to prevent nuclear fragmentation and apoptotic-body formation without affecting DNA fragmentation during apoptosis. Can be used with the PARP Assay.

4667-50-11 100 µL

Cell Culture Control Slides Slides with fi xed apoptotic cells to confi rm a positive reaction with any of the TACS or TACS•XL in situ apoptosis detection kits.

4800-30-21 5 Each

Cobalt Cation For optimizing TdT labeling in TACS in situ apoptosis detection kits. Cobalt is the standard cation for labeling all cell types and tissues.

4810-90-09 3 x 30 µL

CometSlide For use with the CometAssay. 4250-200-03 100 Slides

Cytonin Protease-free, saponin-based permeabilization and blocking reagent designed for use with any of the TACS or TACS•XL apoptosis detection kits.

4876-05-02 2 x 5 mL

Magnesium Cation For optimizing TdT labeling in TACS in situ apoptosis detection kits. Magnesium is useful for tissue that demonstrates high background.

4810-90-10 3 x 30 µL

Manganese Cation For optimizing TdT labeling in TACS in situ apoptosis detection kits. Manganese may increase the signal to noise ratio providing improved labeling.

4810-90-14 3 x 30 µL

NeuroPore Non-proteolytic permeabilization and blocking reagent developed for use with the NeuroTACS kit.

4820-60-01 2 x 5 mL

PARP Activated DNA May be used with the PARP Assay as an activator of Poly(ADP-ribose) Polymerase. 4667-50-06 500 µL

Peroxynitrite For use in the preparation of positive control proteins and cell lysates for immunoreactivity assays.

AR006 5 mL

Poly(ADP-ribose) Polymer/pADPr

Western blotting standard. 4336-100-01 100 µL

Proteinase K Permeabilization reagent. 4800-30-01 30 µL

Streptavidin-Fluorescein For detection of biotin incorporation with fl uorescent detection. 4800-30-14 30 µL

Streptavidin-HRP For detection of biotin incorporation with colorimetric detection. 4800-30-06 30 µL

TACS Blue Label Substrate For use with TACS in situ apoptosis detection kits. 4800-30-BL 30 Tests

TACS Red Label Substrate For use with TACS in situ apoptosis detection kits. 4825-30-RL 30 Tests

TdT Enzyme/Buffer For use with TACS in situ apoptosis detection kits. 9510-01K-EB 1000 U

TdT dNTP Mix Biotinylated nucleotides for use in TACS apoptosis detection. 4810-30-04 30 µL

Tissue Control Slides Slides with fi xed apoptotic tissues to confi rm a positive reaction with any of the TACS or TACS•XL in situ apoptosis detection kits.

4800-30-404800-30-41

2 Each5 Each



Proteome Profi ler™ ArraysProteome Profi ler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment and eliminate the need for multiple immunoprecipitation/Western blot experiments. For more detailed information, please see our website at www.RnDSystems.com/go/ProteomeProfi ler.

Human Apoptosis Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Human Apoptosis Array Kit Contains 4 arrays - each spotted in duplicate with 35 different apoptosis-related antibodies ARY009 1 Kit

Bad, Bax, Bcl-2, Bcl-x, Pro-Caspase-3, Cleaved Caspase-3, Catalase, Claspin, Clusterin, Cytochrome c, FADD, Fas/TNFSF6, HIF-1α, HO-1/HMOX1/HSP32, HO-2/HMOX2, HSP27, HSP60, HSP70, HTRA2/Omi, cIAP-1, cIAP-2, Livin, p21/CIP1/CDNK1A, p27/Kip1, PON2, Phospho-p53 (S15), Phospho-p53 (S46), Phospho-p53 (S392), Phospho-Rad17 (S635), SMAC/Diablo, Survivin, TNF RI/TNFRSF1A, TRAIL R1/DR4, TRAIL R2/DR5, XIAP

1PC

1

2 23

3

44

5

5

6

6

0

10000

20000

30000

40000

50000

60000

Bcl-

x

Cyto

c

HSP

27

HTR

A2/

Om

i

SMAC

/D

iabl

o

XIAP

Pixe

l Int

ensi

ty

Human Phospho-RTK Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit

Contains 4 arrays - each spotted in duplicate with 42 different RTK antibodies ARY001 1 Kit

Axl, Dtk, EGF R, EphA1, EphA2, EphA3, EphA4, EphA6, EphA7, EphB1, EphB2, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FGF R1, FGF R2α, FGF R3, FGF R4, Flt-3, HGF R, IGF-I R, Insulin R, M-CSF R, Mer, MSP R, MuSK, PDGF Rα, PDGF Rβ, c-Ret, ROR1, ROR2, SCF R, Tie-1, Tie-2, TrkA, TrkB, TrkC, VEGF R1, VEGF R2, VEGF R3

A B

C

Detection of Multiple Apoptosis-related Proteins in Cell Lysates using the Proteome Profi ler Human Apoptosis Antibody ArrayA. Cell lysates from human K562 myelogenous leukemia cells were incubated with the Proteome Profi ler Human Apoptosis Antibody Array (Catalog # ARY009). The relative levels of 35 different apoptosis-related proteins were visualized simultaneously by chemiluminescent detection. PC = Positive Control B. Histogram profi les for select analytes were generated by quantifying the mean spot pixel densities from the Apoptosis Antibody Array using image analysis software. C. Analytes and their coordinate locations on the Proteome Profi ler Apoptosis Antibody Array.

COORDINATE TARGET/CONTROL

A1, A2 Positive Control

A23, A24 Positive Control

B1, B2 Bad

B3, B4 Bax

B5, B6 Bcl-2

B7, B8 Bcl-x

B9, B10 Pro-Caspase-3

B11, B12 Cleaved Caspase-3

B13, B14 Catalase

B15, B16 cIAP-1


B17, B18 cIAP-2

B19, B20 Claspin

B21, B22 Clusterin

B23, B24 Cytochrome c

C1, C2 TRAIL R1/DR4

C3, C4 TRAIL R2/DR5

C5, C6 FADD

C7, C8 Fas/TNFSF6

C9, C10 HIF-1α

C11, C12 HO-1/HMOX1/HSP32


C13, C14 HO-2/HMOX2

C15, C16 HSP27

C17, C18 HSP60

C19, C20 HSP70

C21, C22 HTRA2/Omi

C23, C24 Livin

D1, D2 PON2

D3, D4 p21/CIP1/CDNK1A

D5, D6 p27/Kip1

D7, D8 Phospho-p53 (S15)




D13, D14 Phospho-Rad17 (S635)

D15, D16 SMAC/Diablo

D17, D18 Survivin

D19, D20 TNF RI/TNFRSF1A

D21, D22 XIAP

D23, D24 PBS (Negative Control)

E1, E2 Positive Control



Human Phospho-MAPK Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Human Phospho-Mitogen Activated Protein Kinase (MAPK) Array Kit

Contains 4 arrays - each spotted in duplicate with 21 different antibodies recognizing MAPKs and other kinases.

ARY002 1 Kit

Akt1, Akt2, Akt3, Akt pan, p38δ, ERK1, ERK2, GSK-3α/β, GSK-3β, HSP27, JNK1, JNK2, JNK3, JNK pan, MSK2, p38α, p38β, p38γ, p70 S6 Kinase, RSK1, RSK2

Human Phospho-Kinase Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Human Phospho-Kinase Array Kit Contains 4 sets of 2 membranes - each spotted in duplicate with 46 different kinase antibodies ARY003 1 Kit

Akt (S473), Akt (T308), AMPKα1, AMPKα2, β-Catenin, Chk-2, CREB, ERK1/2, FAK, Fgr, Fyn, GSK-3α/β, Hck, HSP27, JNK pan, c-JUN, Lck, Lyn, MEK1/2, MSK1/2, eNOS, p27 (T198), p27 (T157), p38α, p53 (S15), p53 (S46), p53 (S392), p70 S6 Kinase (T229), p70 S6 Kinase (T389), p70 S6 Kinase (T421/S424), Paxillin, PLCγ-1, Pyk2, RSK1/2 (S221), RSK1/2/3 (S380), Src, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5a/b, STAT5b, STAT6, TOR, Yes

Human Phospho-Immunoreceptor Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Human Phospho-Immunoreceptor Array Kit

Contains 4 arrays - each spotted in duplicate with 59 different antibodies recognizing proteins involved in immunoreceptor signaling.

ARY004 1 Kit

2B4, BLAME, BTLA, CD3ε, CD5, CD6, CD28, CD84, CD229, CEACAM-1, CLEC-1, CLEC-2, CRACC, CTLA-4, DCIR, Dectin-1, DNAM-1, Fcε RII, Fcγ RIIA, Fcγ RIIIA, FcRH1, FcRH2, FcRH4, FcRH5, ILT2, ILT3, ILT4, ILT5, ILT6, Integrin β3, KIR2DL4, LAIR-1, LAIR-2, LMIR1, LMIR2, LMIR3, LMIR6, MDL-1, NKp30, NKp44, NKp46, NKp80, NTB-A, PD-1, PECAM, SHIP-1, SHP-1, SHP-2, Siglec-2, Siglec-3, Siglec-5, Siglec-7, Siglec-9, Siglec-10, SIRP-β1, SLAM, TREM-1, TREM-2, TREML1

Human Cytokine Antibody Array, Panel APRODUCT DESCRIPTION CATALOG # SIZE

Human Cytokine Array Kit, Panel A Contains 4 arrays - each spotted in duplicate with 36 different cytokine antibodies ARY005 1 Kit

C5a, CD40 Ligand, G-CSF, GM-CSF, GROα, I-309, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 p70, IL-13, IL-16, IL-17, IL-17E, IL-23, IL-27, IL-32α, IP-10, I-TAC, MCP-1, MIF, MIP-1α, MIP-1β, RANTES, SDF-1, Serpin E1, TNF-α, sTREM-1

Mouse Cytokine Antibody Array, Panel APRODUCT DESCRIPTION CATALOG # SIZE

Mouse Cytokine Array Kit, Panel A Contains 4 arrays - each spotted in duplicate with 40 different cytokine antibodies ARY006 1 Kit

BLC, C5a, G-CSF, GM-CSF, I-309, Eotaxin, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-13, IL-16, IL-17, IL-23, IL-27, IP-10, I-TAC, KC, M-CSF, JE, MCP-5, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, SDF-1, TARC, TIMP-1, TNF-α, TREM-1

Rat Cytokine Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Rat Cytokine Array Kit, Panel A Contains 4 arrays - each spotted in duplicate with 29 different cytokine antibodies ARY008 1 Kit

CINC-1, CINC-2α/β, CINC-3, CNTF, Fractalkine, GM-CSF, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-10, IL-13, IL-17, IP-10, LIX, MIG, MIP-1α, MIP-3α, RANTES, L-Selectin, Thymus Chemokine, TIMP-1, TNF-α, VEGF

Human Angiogenesis Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE

Human Angiogenesis Array Contains 4 arrays - each spotted in duplicate with 55 different angiogenesis antibodies ARY007 1 Kit

Activin A, ADAMTS-1, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin/Plasminogen, Amphiregulin, Artemin, Coagulation Factor III/TF, CXCL16, DPPIV/CD26, EGF, EG-VEGF, Endoglin, Endostatin/Collagen 18, ET-1, FGF acidic, FGF basic, FGF-4, FGF-7/KGF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IL-1β, CXCL8/IL-8, LAP (TGF-β1), Leptin, MCP-1/CCL2, MIP-1α/CCL3, MMP-8, MMP-9, NRG1-β1/HRG1-β1, Pentraxin 3, PD-ECGF, PDGF-AA, PDGF-AB/BB, Persephin, PF4/CXCL4, PlGF, Prolactin, Serpin B5, Serpin E1/PAI-1, Seprin F1/PEDF, TIMP-1, TIMP-4, TSP-1, TSP-2, uPA, Vasohibin, VEGF, VEGF-C


p53 p53

DNA Damage

xx x

x

p53P

PP

P

PP

P

Caspase Recruitment Domain (CARD)

Cytochrome c

BID

SMAC/Diablo HTRA2/Omi

Endo G

AIF

Cytochrome c

Cytochrome c

BID

Caspase-2

Cytochrome c tBID

tBIDtBID

tBID

tBID

tBID

tBID

tBID

tBID

tBID

Bcl-xL

Bcl-2

Bcl-2

Bcl-2

Bax

Bax

Bax Bad

Bad

Bad

Bad

Bad

Bcl-xL

Bcl-xL

Bax

BAK

BAK

BAK

BAK

Bax

Bax

Caspase-8,-10

Caspase-9

Caspase-3

TRAF-2

TRAF-2

TRADD

TRADDTRADD

FADD

FADDFADD

Pro-caspase-8, -10

Pro-caspase-8, -10

TRADD

RIP1

RIP1

DEDDD

Death Domain (DD) Death Effector Domain (DED)

Caspase-7

Caspase-6

IAPs

IAPs

HSPs

Target Molecules (e.g. Actin, Nuclear lamins,ICAD, PARP)

Pro-caspase-7

Pro-caspase-6Pro-caspase-9

Apoptosome

PIDDosome

APAF-1

DNA Fragmentation

ARC

PIDD

CRADD/RAIDD

Pro-caspase-2

Pro-apoptotic: Bad, Bax, BID, Noxa, PUMA, PIDD, othersAnti-apoptotic: 14-3-3, p21, others

Pro-caspase-3

14-3-3

Bad

14-3-3

FADDFADD

TNF-α

TNF RI

Fas Ligand

TRAIL

TWEAK

DR3 (or another TWEAK R)

TRAIL R1TRAIL R2

Fas

FLIP

Pro-caspase-8, -10

DD

DDCARD

Apoptosis-related Molecule Families


Extrinsic & Intrinsic Pathways of Apoptotic Caspase ActivationApoptosis is initiated by an extrinsic or intrinsic death signal which ultimately leads to the activation of intracellular caspases. Multiple protein families are

involved in the signaling pathways that promote or inhibit caspase activation. The extrinsic pathway of caspase activation (green arrows) involves ligand-

binding to cell surface death receptors, belonging to the TNF receptor superfamily, while the intrinsic pathway (purple arrows) involves a loss of the

integrity of the outer mitochondrial membrane which is regulated by members of the Bcl-2 family of proteins. In addition to these two families of proteins,

numerous other intracellular proteins, kinases, and transcription factors play a role in promoting cell death, or acting to prevent it. R&D Systems has

developed and validated a wide variety of research reagents useful for the characterization of these pro- and anti-apoptotic signaling pathways.

Note: this diagram conveys a general overview of selected processes and should neither be considered comprehensive nor defi nitive. The details of these processes are understood to be subject to interpretation.

Extrinsic Pathway of Caspase Activation

Intrinsic Pathway of

Caspase Activation

Caspases & Regulators


.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037318

Caspases are a family of cysteine proteases found in the cytosol

which act as the primary mediators of apoptosis. Mammalian

caspases can be subdivided into three functional groups, apoptotic

initiator caspases (Caspase-2, -8, -9, -10), apoptotic effector

caspases (Caspase-3, -6, -7), and caspases involved in inflammatory

cytokine processing (Caspase-1, -4, -5, and -12L/12S). All caspases

are synthesized as inactive zymogens containing a variable length

pro-domain, followed by a large (20 kDa) and a small subunit

(10 kDa; Figure A). The apoptotic caspases are activated upon the

receipt of either an extrinsic or an intrinsic death signal. The extrin-

sic signaling pathway is initiated by ligand binding to cell surface

death receptors followed by receptor oligomerization. The intrinsic

signaling pathway is activated by conditions such as DNA damage,

which lead to changes in the mitochondrial membrane that result

in the release of pro-apoptotic proteins, such as Cytochrome c and

Smac/Diablo. Signaling initiated by either the extrinsic or the intrin-

sic apoptotic pathway results in the processing and activation of

specific initiator caspases. These caspases subsequently cleave and

activate downstream effector caspases.

Apoptotic initiator caspases are activated in three distinct protein

complexes, the death-inducing signaling complex (DISC), the

apoptosome, and the PIDD osome. Pro - caspase -8 and

Pro-caspase-10 are recruited by way of their death effector do-

mains (DED) to ligand-bound, oligomerized death receptors

(TNF RI, Fas, DR3, TRAIL R1/DR4, TRAIL R2/DR5) by the adaptor pro-

tein, FADD. Clustering of pro-caspases in the cytosol near the death

receptor leads to the formation of the DISC and the subsequent

cleavage of Pro-caspase-8 and Pro-caspase-10. A second death sig-

naling complex is formed following the release of Cytochrome c

from the mitochondria. Cytochrome c interacts with APAF-1 in the

cytosol which recruits Pro-caspase-9 by way of its caspase recruit-

ment domain (CARD). Together these three proteins form a

complex called the apoptosome which leads to the cleavage and

activation of Caspase-9. Formation of a third protein complex leads

to the activation Pro-caspase-2. Following DNA damage, p53 in-

duces the expression of PIDD (p53-induced protein with a death

domain) which associates with the adaptor protein, CRADD/RAIDD

and Pro-caspase-2 to form the PIDDosome. Within this complex,

Pro-caspase-2 is processed and activated. Formation of the DISC,

apoptosome, or the PIDDosome leads to the autocatalytic cleavage

of the respective initiator pro-caspases at aspartic acid residues lo-

cated after the pro-domain, and in between the large and the small

subunits. Upon cleavage, mature caspases form a proteolytically

active heterotetramer consisting of two small and two large sub-

units (Figure B). These activated initiator caspases can then cleave

and activate the effector caspases which proteolytically dismantle

the cell by cleaving specific target substrates, including structural

proteins, DNA repair proteins, and proteins involved in signal

transduction pathways. Caspase activity can be negatively regu-

lated by the FLIP protein and members of the Inhibitors of

Apoptosis (IAP) family.

For more information on Caspase-related products, please visit our

website at www.RnDSystems.com/go/Caspases

Domain Organization and Activation of the Apoptotic Caspases. (A) Initiator pro-caspases consist of a large pro-domain containing either the death effector domains

(DED) or the caspase recruitment domain (CARD), followed by a large (p20) and a small (p10) subunit. Effector pro-caspases contain a significantly shorter pro-domain followed

by the p20 and p10 subunits. (B) Proteolytic cleavage of the pro-caspases at aspartic acid residues located after the pro-domain and in between the large and the small subunit

leads to the formation of a proteolytically active caspase heterotetramer.

For antibody application key, see page 120.

APAF-1ANTIBODIES

ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE

APAF-1aa 10-214

Human None 94408 Mouse IgG2B IP, WB MAB868 100 µg

ARCRECOMBINANT & NATURAL PROTEINS

PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE

ARCWestern Blot Standard

Human Solution without a carrierprotein

None E. coli WBC009 1 Vial

ANTIBODIES


ARCaa 176-195

Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF636 100 µg

ASCANTIBODIES


ASCaa 112-195

Human None Polyclonal Goat IgG WB AF3805 100 µg


For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 19

Detection of Pro-caspase-7 Cleavage in Human

Jurkat and Mouse DA3 Cells. Human Jurkat T cells and

mouse myeloid DA3 cells were either untreated or

treated with staurosporine (STS; 1 µM) for the indicated

times. The pro-caspase and cleaved large subunit forms

of Caspase-7 were detected by Western blot analysis us-

ing anti-human/mouse Caspase-7 monoclonal antibody

(Catalog # MAB823).

Activation of Pro-caspase-3 by Caspase-8. Recombi-

nant human Caspase-8 (Catalog # 705-C8) was

incubated with increasing amounts of Pro-caspase-3

(Catalog #731-C3) for one hour prior to the addition of

the Caspase-3 peptide substrate, DEVD-afc. Caspase-3

activity was determined by monitoring DEVD-afc cleav-

age in a fluorescence plate reader (blue line).

Background activity was measured by monitoring

DEVD-afc cleavage by Pro-caspase-3 in the absence of

active Caspase-8 (red line).

Caspase-3 Expression in Jurkat Cells treated with

Anti-Fas antibodies. Human Jurkat leukemic T cells

were treated with anti-Fas antibodies to induce

apoptosis. Caspase-3 was detected using anti-hu-

man/mouse Active Caspase-3 polyclonal antibody

(Catalog # AF835) followed by Cy3-conjugated anti-rab-

bit secondary antibody (red). Cells were counterstained

with FluoroNissI™ Green (green).


CaspasePEPTIDES & PEPTIDE-BASED ASSAYS

PRODUCT SPECIES SEQUENCE CATALOG # SIZE

Caspase InhibitorPan Specific

Multi-species Z-VAD-FMK FMK001 1 mg

Caspase InhibitorBiotinylatedPan Specific

Multi-species Z-VKD-biotin-FMK FMK011 500 µg

Caspase InhibitorPan Specific

Multi-species Q-VD, Non-omethylated OPH001 1 mg

Caspase Inhibitor ControlPan Specific

Multi-species Z-FA-FMK FMKC01 5 mg

Caspase InhibitorSampler PackPan Specific

Multi-species Z-VAD-FMK, Z-WEHD-FMK, Z-VDVAD-FMK, Z-DEVD-FMK,Z-YVAD-FMK, Z-VEID-FMK, Z-IETD-FMK, Z-LEHD-FMK,Z-AEVD-FMK, Z-LEED-FMK

FMKSP01 100 µg ofeach

Caspase-1ELISAs/ASSAYS

ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE

Caspase-1 Human � Cell Culture Supernate 1.24 pg/mL 6.25-400 pg/mL DCA100 1 Kit

PEPTIDES & PEPTIDE-BASED ASSAYS


Caspase-1Colorimetric CaspaseActivity Assay

Multi-species WEHD BF14100 100 Tests

Caspase-1Fluorometric CaspaseActivity Assay


Caspase-1 Inhibitor Multi-species Z-WEHD-FMK FMK002 1 mg

Caspase-2RECOMBINANT & NATURAL PROTEINS


Caspase-2Active

Human Solution with a carrier protein None E. coli 702-C2-010 10 µg

ANTIBODIES


Caspase-2aa 414-428

Human/Mouse None Polyclonal Rabbit IgG WB AF826 80 µg




Multi-species VDVAD BF8100 100 Tests


Multi-species VDVAD BF5100 100 Tests

Caspase-2 Inhibitor Multi-species Z-VDVAD-FMK FMK003 1 mg



Caspase-3Active


Caspase-3Pro Form

Human Lyophilized without a carrier protein None E. coli 731-C3-005 5 µg


All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).


Caspase-3 continuedANTIBODIES


Caspase-3aa 29-175

Human None 84803 Mouse IgG2B WB MAB707 100 µg

Caspase-3 Human/Mouse None Polyclonal Goat IgG IP, WB AF-605-NA 100 µg

Caspase-3Activeaa 163-175 (human)

Human/Mouse None Polyclonal Rabbit IgG IHC AF835 50 µg

Caspase-3Cleaved (Asp175)

Human/Mouse None 269518 Rabbit IgG IHC, WB MAB835 50 µg

ELISAs/ASSAYS


Caspase-3Active

Human � Cell Extracts 0.1 ng/mL 0.31-20 ng/mL KM300 1 Kit




Multi-species DEVD BF3100 100 Tests


Multi-species DEVD BF1100 100 Tests

Caspase-3 Inhibitor Multi-species Z-DEVD-FMK FMK004 1 mg

Caspase-4PEPTIDES & PEPTIDE-BASED ASSAYS


Caspase-4 Inhibitor Multi-species Z-YVAD-FMK FMK005 1 mg




Multi-species VEID BF9100 100 Tests


Multi-species VEID BF6100 100 Tests

Caspase-6 Inhibitor Multi-species Z-VEID-FMK FMK006 1 mg



Caspase-7Active


ANTIBODIES


Caspase-7 Human/Mouse None MCH3101.62 Mouse IgG1 WB MAB823 100 µg

Caspase-7aa 271-285 (human)








Caspase-8Active


ANTIBODIES


Caspase-8aa 234-391

Human None 84131 Mouse IgG2A WB MAB704 100 µg

Caspase-8 Human/Mouse None Polyclonal Rabbit IgG WB AF1650 100 µg

Caspase-8 Human/Mouse None Polyclonal Goat IgG WB AF705 100 µg




Multi-species IETD BF4100 100 Tests


Multi-species IETD BF2100 100 Tests

Caspase-8 Inhibitor Multi-species Z-IETD-FMK FMK007 1 mg

Caspase-9ANTIBODIES


Caspase-9aa 1-134

Human None LAP6 Mouse IgG1 ELISA(Cap), IHC, WB MAB8301 100 µg

Caspase-9aa 139-330

Human None Polyclonal Goat IgG IHC, WB AF8301 100 µg




Multi-species LEHD BF10100 100 Tests


Multi-species LEHD BF7100 100 Tests

Caspase-9 Inhibitor Multi-species Z-LEHD-FMK FMK008 1 mg



Caspase-10Active Isoform a

Human Solution without a carrier protein None E. coli 834-CP-010 10 µg

ANTIBODIES


Caspase-10Isoform a


Caspase-10Isoform baa 487-504

Human None Polyclonal Rabbit IgG WB AF834 100 µg




Caspase-10 continuedPEPTIDES & PEPTIDE-BASED ASSAYS





Multi-species AEVD BF16100 100 Tests

Caspase-10 Inhibitor Multi-species Z-AEVD-FMK FMK009 1 mg

Caspase-12ANTIBODIES


Caspase-12 Mouse/Rat None Polyclonal Rabbit IgG WB AF1456 100 µg



Caspase-13 Inhibitor Multi-species Z-LEED-FMK FMK010 1 mg

FLIPRECOMBINANT & NATURAL PROTEINS


FLIPWestern Blot Standard

Human Lyophilized without a carrier protein None E. coli WBC001 1 Vial

ANTIBODIES


FLIPaa 432-450


NALP2ANTIBODIES


NALP2aa 1-208

Human None Polyclonal Sheep IgG WB AF4684 100 µg





Inhibitors of Apoptosis (IAPs) & Regulators



As their name implies, a primary role for members of the Inhibitors

of Apoptosis (IAP) family lies in their ability to suppress apoptosis.

This occurs through the inhibition of pro-apoptotic members of

the Caspase family. Some IAPs also act as ubiquitin ligases, target-

ing specific proteins for proteasomal degradation. IAP proteins are

characterized structurally by the presence of at least one BIR

(Baculoviral IAP Repeat) domain. Binding of the BIR domain of IAPs

to active caspases inhibits caspase activity. Eight IAPs have been

identified in humans including Apollon/BRUCE, cIAP-1, cIAP-2, ILP-2,

Livin, NAIP, Survivin, and XIAP. The activities of IAPs are themselves

suppressed by mitochondrial proteins released into the cytosol dur-

ing apoptosis, such as HTRA2/Omi and Smac/Diablo. These proteins

bind to the BIR domain of IAPs and reduce their ability to interact

with caspases.

For more information on IAP-related products, please visit our website

at www.RnDSystems.com/go/IAPs

The IAP Family of Proteins. Members of the Inhibitors of Apoptosis (IAP) family of proteins contain one or more baculoviral IAP repeat (BIR) domain through which they can di-

rectly bind to Caspase-3, -7, or -9 to inhibit their activity. Some IAPs also contain domains that are characteristic of enzymes involved in ubiquitin-mediated proteasomal

degradation, such as the UBC domain found in E2 ubiquitin-conjugating enzymes, or the RING finger domain found in many E3 ubiquitin ligases. In addition, cIAP-1 and cIAP-2,

like Pro-caspase-2 and Pro-caspase-9, contain a caspase-recruitment domain (CARD).

Inhibition of Caspase Activity by XIAP. Recombinant

human Caspase-3 (Catalog # 707-C3; purple line),

Caspase-7 (Catalog # 823-C7; gray line), or Cytochrome c

and dATP-activated Jurkat cell extracts (green line) were

mixed with increasing concentrations of recombinant

human XIAP (Catalog # 822-XF) prior to the addition of

the caspase peptide substrate, DEVD-afc. DEVD-afc

cleavage was monitored in a fluorescence plate reader.

Survivin in Human THP-1 and HeLa Cell Lysates. Hu-

man THP-1 monocytic leukemia cells and human HeLa

cells were either untreated (-) or treated (+) with

200 ng/mL nocodazole for 16 hours. Cells were lysed

and Survivin was assayed using the human Survivin

DuoSet® IC ELISA (Catalog # DYC647). Similar results

were obtained by Western blot analysis using anti-hu-

man Survivin polyclonal antibody (Catalog # AF886).

cIAP-1 in Human Lymphoma Tissue. cIAP-1 was de-

tected in paraffin-embedded human lymphoma tissue

sections using anti-human/mouse cIAP-1 polyclonal

antibody (Catalog # AF818). Tissues were stained using

anti-rabbit HRP-DAB Cell and Tissue Staining Kit

(Catalog # CTS005; brown) and counterstained with

hematoxylin (blue).

cIAPANTIBODIES


cIAPPan Specific

Human/Mouse None 315301 Mouse IgG2A WB MAB3400 100 µg

cIAP-1/HIAP-2RECOMBINANT & NATURAL PROTEINS


cIAP-1/HIAP-2 Human Solution without a carrier protein None E. coli 818-IA-050 50 µg

ANTIBODIES


cIAP-1/HIAP-2 Human None Polyclonal Goat IgG IHC, WB AF8181 100 µg

cIAP-1/HIAP-2aa 527-546 (human)


cIAP-2/HIAP-1RECOMBINANT & NATURAL PROTEINS


cIAP-2/HIAP-1 Human Solution without a carrier protein None E. coli 817-P2-050 50 µg

ANTIBODIES


cIAP-2/HIAP-1 Human None Polyclonal Goat IgG IHC, WB AF8171 100 µg

cIAP-2/HIAP-1 Human None 315304 Mouse IgG1 IHC, WB MAB817 100 µg

HTRA2/OmiRECOMBINANT & NATURAL PROTEINS


HTRA2/Omi Human Solution without a carrier protein None E. coli 1458-HT-100 100 µg

ANTIBODIES


HTRA2/Omi Human None 229926 Mouse IgG2B IHC, WB MAB1458 100 µg

HTRA2/Omi Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1458 100 µg

LivinRECOMBINANT & NATURAL PROTEINS


Livin� Isoform

Human Solution without a carrier protein None E. coli 1161-LV-100 100 µg

Livin� Isoform

Human Solution without a carrier protein None E. coli 787-LV-100 100 µg

ANTIBODIES


Livin Human None Polyclonal Goat IgG WB AF1161 100 µg

Livin Human Biotin Polyclonal Goat IgG WB BAF1161 50 µg





Livin continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS

ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*

LivinTotal Protein

Human 31.2 - 2000 pg/mL DYC1161-2 2 Plates

* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.

NAIPANTIBODIES

ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZ

NAIPaa 503-520


SMAC/DiabloRECOMBINANT & NATURAL PROTEINS


SMAC/Diablo Human Solution without a carrier protein None E. coli 789-SM-100 100 µg

ANTIBODIES


SMAC/Diablo Human None Polyclonal Rabbit IgG WB AF789 100 µg

SMAC/Diablo Human None Polyclonal Goat IgG IHC, WB AF7891 100 µg

SMAC/Diablo Human Biotin Polyclonal Goat IgG WB BAF789 50 µg

DUOSET IC ELISA DEVELOPMENT SYSTEMS


SMAC/DiabloTotal Protein



SurvivinRECOMBINANT & NATURAL PROTEINS


SurvivinWestern Blot Standard

Human Lyophilized without a carrier protein None N/A WBC010B 1 Vial

ANTIBODIES


Survivin Human None Polyclonal Goat IgG FC, WB AF6471 100 µg

Survivin Human None Polyclonal Rabbit IgG IHC, WB AF886 100 µg

Survivin Human Biotin Polyclonal Goat IgG FC, WB BAF6471 50 µg

Survivin Human Fluorescein 91630 Mouse IgG1 FC IC6472F 100 Tests

Survivin Human PE 91630 Mouse IgG1 FC IC6472P 100 Tests

Survivin Human None 91630 Mouse IgG1 FC MAB886 100 µg




Survivin continuedELISAs/ASSAYS


Survivin Human � Serum

� EDTA and Heparin Plasma

� Cell Culture Supernate

� Urine

9.96 pg/mL 31.2-2000 pg/mL DSV00 1 Kit

SurvivinTotal ProteinSurveyor IC ELISA

Human � Cell Lysate N/A 62.5-4000 pg/mL SUV647 1 Kit



SurvivinTotal Protein



PRIMER PAIRS

PRODUCT SPECIES CATALOG # SIZE

Survivin Human RDP-204-025 25 Tests

XIAPRECOMBINANT & NATURAL PROTEINS


XIAPBIR2 Domain

Human Solution without a carrier protein None E. coli 786-XB-050 50 µg

XIAPBIR3 Domain

Human Solution without a carrier protein None E. coli 895-XB-050 50 µg

XIAPFull Length

Human Solution without a carrier protein None E. coli 822-XF-050 50 µg

XIAPWestern Blot Standard

Human Solution without a carrier protein None E. coli WBC002 1 Vial

ANTIBODIES


XIAP Human None 117318 Mouse IgG1 B/N, IHC MAB8221 100 µg

XIAP Human/Mouse None 117320 Mouse IgG2A WB MAB822 100 µg

XIAP Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF8221 100 µg



XIAPTotal Protein

Human 312 - 20000 pg/mL DYC822-2 2 Plates








The Tumor Necrosis Factor (TNF) superfamily is a group of proteins

with important functions in immunity, inflammation, cell prolifera-

tion, differentiation, and apoptosis. Most members of the TNF

superfamily are type II transmembrane proteins. The extrinsic path-

way of caspase activation is mediated by death receptors

belonging to the TNF superfamily of receptors, including TNF RI,

Fas, DR3, TRAIL R1/DR4, and TRAIL R2/DR5. Ligand binding to

these receptors triggers the recruitment of adaptor proteins which

subsequently recruit pro-caspases, resulting in their activation. For

example, TNF-� binds to TNF RI and recruits the adaptor proteins,

TRADD and FADD leading to the subsequent formation of two sig-

naling complexes. One complex contains Pro-caspase-8 and

Pro-caspase-10 and culminates in the activation of the caspase cas-

cade leading to apoptosis. The second complex, containing TRADD

and TRAF-2, promotes the activation of the NF�B and MAPK path-

ways. Other members of the TNF superfamily are involved in

making specific cells more susceptible to death receptor-mediated

cell death, or in preventing apoptotic signaling.

For more information on TNF superfamily products, please visit our

website at www.RnDSystems.com/go/TNFSF

Death Receptors and Adaptor Proteins Involved in Apoptotic Signaling. Receptors of the TNF superfamily, including TNF RI, Fas, TRAIL R1 and R2, and DR3 activate

apoptotic signaling cascades upon ligand binding by recruiting adaptor proteins containing death domains (DD), such as TRADD and FADD. These adaptor proteins recruit

Pro-caspase-8, and -10 through their shared death effector domains (DED).

Fas Ligand-induced Apoptosis of Human Jurkat Cells

& Antibody Neutralization. The ability of recombinant

human Fas Ligand (Catalog # 126-FL) to induce

apoptosis was determined by measuring 3H-thymidine

incorporation into the DNA of human Jurkat T cells incu-

bated with increasing concentrations of Fas Ligand (blue

line). This effect was neutralized by incubating the Fas

Ligand (2 ng/mL) with increasing concentrations of

anti-human Fas Ligand polyclonal antibody (Catalog #

AF126) prior to its addition to Jurkat cells (orange line).

Intracellular Detection of TNF-� in EL-4 Cells by Flow

Cytometry. EL-4 mouse lymphoma cells were stained

with PE-conjugated anti-mouse TNF-� monoclonal anti-

body (Catalog # IC410P; filled histogram) or with

PE-conjugated rat IgG isotype control antibody (Catalog

# IC005P; open histogram)

TRAIL-induced Cytoxicity in Mouse L929 Cells. The

cytotoxicity of recombinant human TRAIL (Catalog #

375-TL) was determined by incubating mouse L929

fibroblast cells with increasing concentrations of the

cross-linked protein in the presence of the metabolic in-

hibitor actinomycin D. The cytotoxic effect induced by

TRAIL (20 ng/mL) was neutralized by incubating the

protein with increasing concentrations of anti-human

TRAIL/TNFSF10 polyclonal antibody (Catalog # AF375)

prior to its addition to L929 cells (inset).

TNF Superfamily

Extrinsic Pathway of Caspase Activation

4-1BB/TNFRSF9RECOMBINANT & NATURAL PROTEINS


4-1BB/TNFRSF9/Fc Chimera Human Lyophilized without a carrier protein None NS0 838-4B-100 100 µg

4-1BB/TNFRSF9/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 937-4B-050 50 µg

ANTIBODIES


4-1BB/TNFRSF9 Human None Polyclonal Goat IgG ELISA(Cap), FA, FC,IHC, WB

AF838 100 µg

4-1BB/TNFRSF9 Human Biotin Polyclonal Goat IgG ELISA(Det), FC, IHC,WB

BAF838 50 µg

4-1BB/TNFRSF9 Human PE 145501 Mouse IgG2B FC FAB838P 100 Tests

4-1BB/TNFRSF9 Human None 145501 Mouse IgG2B FC, WB MAB838 500 µg

4-1BB/TNFRSF9 Mouse None Polyclonal Goat IgG FA, FC, WB AF937 100 µg

4-1BB/TNFRSF9 Mouse Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF937 50 µg

4-1BB/TNFRSF9 Mouse None 158332 Rat IgG2A ELISA(Cap), WB MAB937 500 µg

4-1BB/TNFRSF9 Mouse None 158321 Rat IgG2A FA MAB9371 500 µg

4-1BB/TNFRSF9 Mouse None 158306 Rat IgG2B B/N, FC, WB MAB9372 500 µg

DUOSET ELISA DEVELOPMENT SYSTEMS


4-1BB/TNFRSF9 Human 31.2 - 2000 pg/mL DY838 15 Plates

4-1BB/TNFRSF9 Mouse 31.2 - 2000 pg/mL DY937 15 Plates


4-1BB Ligand/TNFSF9RECOMBINANT & NATURAL PROTEINS


4-1BB Ligand/TNFSF9 Human Lyophilized with a carrier protein None E. coli 2295-4L-025 25 µg

4-1BB Ligand/TNFSF9 Mouse Lyophilized with a carrier protein None NS0 1246-4L-025 25 µg

ANTIBODIES


4-1BB Ligand/TNFSF9 Human PE 282220 Mouse IgG2B FC FAB2295P 100 Tests

4-1BB Ligand/TNFSF9 Human None 282220 Mouse IgG2B FC, WB MAB2295 100 µg

4-1BB Ligand/TNFSF9aa 71-254

Human None Polyclonal Goat IgG FC, WB AF2295 100 µg

4-1BB Ligand/TNFSF9aa 71-254

Human Biotin Polyclonal Goat IgG FC, WB BAF2295 50 µg

4-1BB Ligand/TNFSF9 Mouse None Polyclonal Goat IgG WB AF1246 100 µg

4-1BB Ligand/TNFSF9 Mouse Biotin Polyclonal Goat IgG WB BAF1246 50 µg

4-1BB Ligand/TNFSF9 Mouse APC 203942 Rat IgG2B FC FAB1246A 100 Tests

4-1BB Ligand/TNFSF9 Mouse Fluorescein 203942 Rat IgG2B FC FAB1246F 100 Tests

4-1BB Ligand/TNFSF9 Mouse PE 203942 Rat IgG2B FC FAB1246P 100 Tests

4-1BB Ligand/TNFSF9 Mouse None 203942 Rat IgG2B FC, WB MAB1246 500 µg




TNF Superfamily

APRIL/TNFSF13RECOMBINANT & NATURAL PROTEINS


APRIL/TNFSF13 Human Lyophilized with a carrier protein None NS0 884-AP-010 10 µg

ANTIBODIES


APRIL/TNFSF13 Human None Polyclonal Goat IgG FC, WB AF884 100 µg

APRIL/TNFSF13 Human Biotin Polyclonal Goat IgG FC, WB BAF884 50 µg

APRIL/TNFSF13 Human None 101115 Mouse IgG2A WB MAB884 500 µg

PRIMER PAIRS


APRIL/TNFSF13 Human RDP-127-025 25 Tests

BAFF/TNFSF13BRECOMBINANT & NATURAL PROTEINS


BAFF/TNFSF13B Human Lyophilized with a carrier protein None NS0 2149-BF-010 10 µg

BAFF/TNFSF13B Mouse Lyophilized with a carrier protein None NS0 2106-BF-010 10 µg

ANTIBODIES


BAFF/TNFSF13B Human None Polyclonal Goat IgG IHC, WB AF124 100 µg

BAFF/TNFSF13B Human Biotin Polyclonal Goat IgG WB BAF124 50 µg

BAFF/TNFSF13B Human Fluorescein 137314 Mouse IgG1 FC IC1241F 100 Tests

BAFF/TNFSF13B Human PE 137314 Mouse IgG1 FC IC1241P 100 Tests

BAFF/TNFSF13B Human None 148725 Mouse IgG2B B/N MAB124 500 µg

BAFF/TNFSF13B Human None 137314 Mouse IgG1 FC, WB MAB1241 500 µg

BAFF/TNFSF13B Mouse None Polyclonal Goat IgG B/N, WB AF2106 100 µg

BAFF/TNFSF13B Mouse Biotin Polyclonal Goat IgG IHC, WB BAF2106 50 µg

BAFF/TNFSF13B Mouse PE 121808 Rat IgG2A FC IC1357P 100 Tests

BAFF/TNFSF13B Mouse None 121808 Rat IgG2A FC, WB MAB1357 500 µg

ELISAs/ASSAYS


BAFF/TNFSF13B Human � Serum (S)

� EDTA and Heparin Plasma (P)

� Cell Culture Supernate (C)

11.9 pg/mL (S P)6.67 pg/mL (C)

62.5-4000 pg/mL (S P)31.2-2000 pg/mL (C)

DBLYS0 1 Kit

BAFF/TNFSF13B Mouse � Serum



7.8 pg/mL 46.9-3000 pg/mL MBLYS0 1 Kit

PRIMER PAIRS


BAFF/TNFSF13B Human RDP-124-025 25 Tests




BAFF R/TNFRSF13CRECOMBINANT & NATURAL PROTEINS


BAFF R/TNFRSF13C/Fc Chimera

Human Lyophilized without a carrier protein None NS0 1162-BR-050 50 µg

BAFF R/TNFRSF13C/Fc Chimera

Mouse Lyophilized without a carrier protein None NS0 1357-BR-050 50 µg

ANTIBODIES


BAFF R/TNFRSF13C Human None Polyclonal Goat IgG B/N, FC, WB AF1162 100 µg

BAFF R/TNFRSF13C Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), WB AF1357 100 µg

BAFF R/TNFRSF13C Mouse Biotin Polyclonal Goat IgG WB BAF1357 50 µg

BAFF R/TNFRSF13C Mouse Fluorescein 204406 Rat IgG2A FC FAB1755F 100 Tests

BAFF R/TNFRSF13C Mouse None 204406 Rat IgG2A FC MAB1755 500 µg



BAFF R/TNFRSF13C Mouse 78.1 - 5000 pg/mL DY1357 15 Plates


BCMA/TNFRSF17RECOMBINANT & NATURAL PROTEINS


BCMA/TNFRSF17/Fc Chimera

Human Lyophilized without a carrier protein None NS0 193-BC-050 50 µg

BCMA/TNFRSF17/Fc Chimera

Mouse Lyophilized without a carrier protein None NS0 593-BC-050 50 µg

ANTIBODIES


BCMA/TNFRSF17 Human None Polyclonal Goat IgG B/N, ELISA(Cap), FC,WB

AF193 100 µg

BCMA/TNFRSF17 Human Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF193 50 µg

BCMA/TNFRSF17 Human Fluorescein Polyclonal Goat IgG FC FAB193F 100 Tests

BCMA/TNFRSF17 Human PE Polyclonal Goat IgG FC FAB193P 100 Tests

BCMA/TNFRSF17 Human None 335004 Rat IgG2A B/N, WB MAB193 500 µg

BCMA/TNFRSF17 Mouse None Polyclonal Goat IgG B/N, WB AF593 100 µg

BCMA/TNFRSF17 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF593 50 µg

BCMA/TNFRSF17 Mouse Fluorescein 161616 Rat IgG1 FC FAB593F 100 Tests

BCMA/TNFRSF17 Mouse None 161616 Rat IgG1 FC MAB593 500 µg

BCMA/TNFRSF17 Mouse None 161608 Rat IgG2A B/N, ELISA(Cap), WB MAB5931 500 µg



BCMA/TNFRSF17 Human 31.2 - 2000 pg/mL DY193 15 Plates


PRIMER PAIRS


BCMA/TNFRSF17 Human RDP-140-025 25 Tests




TNF Superfamily

CD27/TNFRSF7RECOMBINANT & NATURAL PROTEINS


CD27/TNFRSF7/Fc Chimera Human Lyophilized without a carrier protein None NS0 382-CD-100 100 µg

CD27/TNFRSF7/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 574-CD-050 50 µg

ANTIBODIES


CD27/TNFRSF7 Human None Polyclonal Goat IgG B/N, FC, WB AF382 100 µg

CD27/TNFRSF7 Human Biotin Polyclonal Goat IgG FC, IHC, WB BAF382 50 µg

CD27/TNFRSF7 Human APC 57703 Mouse IgG1 FC FAB382A 100 Tests

CD27/TNFRSF7 Human Fluorescein 57703 Mouse IgG1 FC FAB382F 100 Tests

CD27/TNFRSF7 Human PE 57703 Mouse IgG1 FC FAB382P 100 Tests

CD27/TNFRSF7 Human None 57703 Mouse IgG1 B/N, FC, WB MAB382 500 µg

CD27/TNFRSF7 Mouse None Polyclonal Goat IgG FC, WB AF574 100 µg

CD27/TNFRSF7 Mouse Biotin Polyclonal Goat IgG WB BAF574 50 µg

CD27/TNFRSF7 Mouse Biotin 137925 Rat IgG2A ELISA(Det) BAM5741 250 µg

CD27/TNFRSF7 Mouse None 137910 Rat IgG2A FC, WB MAB574 100 µg

CD27/TNFRSF7 Mouse None 137915 Rat IgG2A ELISA(Cap), WB MAB5741 500 µg



CD27/TNFRSF7 Mouse 62.5 - 4000 pg/mL DY574 15 Plates

* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .

PRIMER PAIRS


CD27/TNFRSF7 Human RDP-301-025 25 Tests

CD27 Ligand/TNFSF7RECOMBINANT & NATURAL PROTEINS


CD27 Ligand/TNFSF7 Mouse Lyophilized with a carrier protein None NS0 783-CL-050 50 µg

ANTIBODIES


CD27 Ligand/TNFSF7 Human None 301731 Mouse IgG2B IHC, WB MAB2738 100 µg

CD27 Ligand/TNFSF7 Mouse None Polyclonal Goat IgG B/N, WB AF783 100 µg

CD27 Ligand/TNFSF7 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF783 50 µg

CD27 Ligand/TNFSF7 Mouse None 118510 Rat IgG1 B/N, ELISA(Cap) MAB783 500 µg



CD27 Ligand/TNFSF7 Mouse 31.2 - 2000 pg/mL DY783 15 Plates


PRIMER PAIRS


CD27 Ligand/TNFSF7 Mouse RDP-302-025 25 Tests




ss





ANTIBODIES


CD30/TNFRSF8 Human None Polyclonal Goat IgG FA, WB AF229 100 µg

CD30/TNFRSF8 Human Biotin Polyclonal Goat IgG WB BAF229 50 µg

CD30/TNFRSF8 Human Fluorescein 81337 Mouse IgG2B FC FAB229F 100 Tests

CD30/TNFRSF8 Human PE 81337 Mouse IgG2B FC FAB229P 100 Tests

CD30/TNFRSF8 Human None 81337 Mouse IgG2B FA, FC, WB MAB229 500 µg

CD30/TNFRSF8 Human None 81316 Mouse IgG1 WB MAB2291 500 µg

CD30/TNFRSF8 Mouse None Polyclonal Goat IgG FA, IHC, WB AF852 100 µg

CD30/TNFRSF8 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF852 50 µg

CD30/TNFRSF8 Mouse None 115705 Rat IgG2A ELISA(Cap), WB MAB8521 500 µg



CD30/TNFRSF8 Mouse 15.6 - 1000 pg/mL DY852 15 Plates


PRIMER PAIRS



CD30/TNFRSF8 Mouse/Rat RDP-304-025 25 Tests



CD30 Ligand/TNFSF8 Human Lyophilized without a carrier protein None NS0 1028-CL-050 50 µg

CD30 Ligand/TNFSF8 Mouse Lyophilized with a carrier protein None NS0 732-CL-050 50 µg

ANTIBODIES


CD30 Ligand/TNFSF8 Human None Polyclonal Goat IgG FC, WB AF1028 100 µg

CD30 Ligand/TNFSF8 Human Biotin Polyclonal Goat IgG WB BAF1028 50 µg

CD30 Ligand/TNFSF8 Human APC 116614 Mouse IgG2B FC FAB1028A 100 Tests

CD30 Ligand/TNFSF8 Human PE 116614 Mouse IgG2B FC FAB1028P 100 Tests

CD30 Ligand/TNFSF8 Human None 116614 Mouse IgG2B B/N, FC MAB1028 500 µg

CD30 Ligand/TNFSF8 Human None 116615 Mouse IgG2A WB MAB774 500 µg

CD30 Ligand/TNFSF8 Mouse None Polyclonal Goat IgG B/N, WB AF732 100 µg

CD30 Ligand/TNFSF8 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF732 50 µg

CD30 Ligand/TNFSF8 Mouse None 82414 Rat IgG2A ELISA(Cap), WB MAB732 500 µg




TNF Superfamily

CD30 Ligand/TNFSF8 continuedDUOSET ELISA DEVELOPMENT SYSTEMS




PRIMER PAIRS


CD30 Ligand/TNFSF8 Human RDP-305-025 25 Tests





ANTIBODIES


CD40/TNFRSF5 Human None Polyclonal Goat IgG FA, WB AF632 100 µg

CD40/TNFRSF5 Human Biotin Polyclonal Goat IgG WB BAF632 50 µg

CD40/TNFRSF5 Human APC 82111 Mouse IgG2B FC FAB6321A 100 Tests

CD40/TNFRSF5 Human PE 82111 Mouse IgG2B FC FAB6321P 100 Tests

CD40/TNFRSF5 Human None 82105 Mouse IgG2B WB MAB632 500 µg

CD40/TNFRSF5 Human None 82111 Mouse IgG2B FA, FC, IHC MAB6321 500 µg

CD40/TNFRSF5 Human None 82102 Mouse IgG2B B/N, WB MAB6322 500 µg

CD40/TNFRSF5 Mouse None Polyclonal Goat IgG WB AF440 100 µg

CD40/TNFRSF5 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF440 50 µg

CD40/TNFRSF5 Mouse Biotin 1C10 Rat IgG2A IF, IP BAM440 250 µg

CD40/TNFRSF5 Mouse PE 65924 Rat IgG1 FC FAB4401P 100 Tests

CD40/TNFRSF5 Mouse None 1C10 Rat IgG2A FA, FC, IP MAB440 500 µg

CD40/TNFRSF5 Mouse None 65924 Rat IgG1 ELISA(Cap), FC, WB MAB4401 500 µg



CD40/TNFRSF5 Mouse 15.6 - 1000 ng/mL DY097 15 Plates


PRIMER PAIRS








CD40 Ligand/TNFSF5aa 108-261

Human Lyophilized with a carrier protein None E. coli 617-CL-050 50 µg

CD40 Ligand/TNFSF5Histidine-taggedaa 108-261

Human Lyophilized with a carrier protein None E. coli 2706-CL-025 25 µg

CD40 Ligand/TNFSF5aa 61-260

Mouse Lyophilized with a carrier protein None NS0 1163-CL-025 25 µg

ANTIBODIES


CD40 Ligand/TNFSF5 Human None Polyclonal Goat IgG FC, IHC, WB AF617 100 µg

CD40 Ligand/TNFSF5 Human Biotin Polyclonal Goat IgG FC, WB BAF617 50 µg

CD40 Ligand/TNFSF5 Human APC 40804 Mouse IgG2B FC FAB617A 100 Tests

CD40 Ligand/TNFSF5 Human Fluorescein 40804 Mouse IgG2B FC FAB617F 100 Tests

CD40 Ligand/TNFSF5 Human PE 40804 Mouse IgG2B FC FAB617P 100 Tests

CD40 Ligand/TNFSF5 Human None 40804 Mouse IgG2B B/N, FC, WB MAB617 500 µg

CD40 Ligand/TNFSF5 Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,WB

AF1163 100 µg

CD40 Ligand/TNFSF5 Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1163 50 µg

CD40 Ligand/TNFSF5 Mouse APC 208109 Rat IgG2A FC FAB1163A 100 Tests

CD40 Ligand/TNFSF5 Mouse Fluorescein 208109 Rat IgG2A FC FAB1163F 100 Tests

CD40 Ligand/TNFSF5 Mouse PE 208109 Rat IgG2A FC FAB1163P 100 Tests

CD40 Ligand/TNFSF5 Mouse None 208109 Rat IgG2A B/N, FC MAB1163 500 µg

ELISAs/ASSAYS


CD40 Ligand/TNFSF5 Human � Serum

� Platelet-poor EDTA andHeparin Plasma


10.1 pg/mL 62.5-4000 pg/mL DCDL40 1 Kit



CD40 Ligand/TNFSF5 Human 15.6 - 1000 pg/mL DY617 15 Plates



PRIMER PAIRS


CD40 Ligand/TNFSF5 Human RDP-307-025 25 Tests

CD40 Ligand/TNFSF5 Mouse/Rat RDP-308-025 25 Tests

CRMBRECOMBINANT & NATURAL PROTEINS


CRMB Viral Lyophilized with a carrier protein None NS0 4778-CR-010 10 µg




TNF Superfamily

DcR3/TNFRSF6BRECOMBINANT & NATURAL PROTEINS


DcR3/TNFRSF6B/Fc Chimera Human Lyophilized without a carrier protein None Sf 21 142-DC-100 100 µg

ANTIBODIES


DcR3/TNFRSF6B Human None Polyclonal Goat IgG B/N, IHC, WB AF142 100 µg

DcR3/TNFRSF6B Human Biotin Polyclonal Goat IgG IHC, WB BAF142 50 µg

DcR3/TNFRSF6B Human None 229812 Mouse IgG1 B/N, WB MAB2135 500 µg

DcR3/TNFRSF6B Human None 229817 Mouse IgG1 ELISA(Cap), WB MAB21351 500 µg



DcR3/TNFRSF6B Human 187 - 12000 pg/mL DY142 15 Plates


PRIMER PAIRS


DcR3/TNFRSF6B Human RDP-115-025 25 Tests

DcTRAIL R1/TNFRSF23ANTIBODIES


DcTRAIL R1/TNFRSF23 Mouse None Polyclonal Goat IgG IHC, WB AF2378 100 µg

DcTRAIL R1/TNFRSF23 Mouse Biotin Polyclonal Goat IgG WB BAF2378 50 µg

DcTRAIL R1/TNFRSF23 Mouse Biotin 270012 Rat IgG2A IHC BAM2378 100 µg

DcTRAIL R1/TNFRSF23 Mouse None 270012 Rat IgG2A WB MAB2378 100 µg

DcTRAIL R1/TNFRSF23 Mouse None 270028 Rat IgG2A WB MAB23781 100 µg

DcTRAIL R2/TNFRSF22RECOMBINANT & NATURAL PROTEINS


DcTRAIL R2/TNFRSF22 Mouse Lyophilized with a carrier protein None NS0 1816-DT-050 50 µg

ANTIBODIES


DcTRAIL R2/TNFRSF22 Mouse None Polyclonal Goat IgG WB AF1816 100 µg

DcTRAIL R2/TNFRSF22 Mouse Biotin Polyclonal Goat IgG WB BAF1816 50 µg

DcTRAIL R2/TNFRSF22 Mouse None 263310 Rat IgG2B WB MAB1816 100 µg




DR3/TNFRSF25RECOMBINANT & NATURAL PROTEINS


DR3/TNFRSF25/Fc Chimera Human Lyophilized without a carrier protein None NS0 943-D3-050 50 µg

DR3/TNFRSF25/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 2437-D3-050 50 µg

ANTIBODIES


DR3/TNFRSF25 Human None Polyclonal Goat IgG FC, WB AF943 100 µg

DR3/TNFRSF25 Human Biotin Polyclonal Goat IgG FC, WB BAF943 50 µg

DR3/TNFRSF25 Human None 59204 Mouse IgG1 WB MAB943 500 µg

DR3/TNFRSF25 Mouse None Polyclonal Goat IgG IHC, WB AF2437 100 µg

DR3/TNFRSF25 Mouse Biotin Polyclonal Goat IgG WB BAF2437 50 µg

PRIMER PAIRS


DR3/TNFRSF25 Human RDP-65-025 25 Tests

DR6/TNFRSF21RECOMBINANT & NATURAL PROTEINS


DR6/TNFRSF21/Fc Chimera Human Lyophilized without a carrier protein None Sf 21 144-DR-100 100 µg

ANTIBODIES


DR6/TNFRSF21 Human None Polyclonal Goat IgG ELISA(Cap), WB AF144 100 µg

DR6/TNFRSF21 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF144 50 µg

DR6/TNFRSF21 Human None 103402 Mouse IgG1 WB MAB1441 500 µg



DR6/TNFRSF21 Human 62.5 - 4000 pg/mL DY144 15 Plates

* DuoSet IC Kits also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits also available in 45 plate Economy Packs.

PRIMER PAIRS


DR6/TNFRSF21 Human/Mouse/Rat RDP-123-025 25 Tests

EDARECOMBINANT & NATURAL PROTEINS


EDA Human Lyophilized with a carrier protein None NS0 3944-ED-010 10 µg

EDA Mouse Lyophilized with a carrier protein None NS0 191-ED-010 10 µg

ANTIBODIES


EDA Human Biotin 174817 Mouse IgG2B ELISA(Det) BAM9221 100 µg

EDA Human None 174806 Mouse IgG2B ELISA(Cap) MAB9222 500 µg




TNF Superfamily

EDA continuedDUOSET ELISA DEVELOPMENT SYSTEMS


EDA Human 93.75 - 6000 pg/mL DY922 15 Plates


EDA-A2RECOMBINANT & NATURAL PROTEINS


EDA-A2aa 179-389

Human Lyophilized with a carrier protein None NS0 922-ED-010 10 µg

ANTIBODIES


EDA-A2aa 179-389

Human None Polyclonal Goat IgG B/N, WB AF922 100 µg

EDA-A2aa 179-389

Human Biotin Polyclonal Goat IgG WB BAF922 50 µg

EDA-A2aa 179-389

Human None 174813 Mouse IgG1 WB MAB922 500 µg

EDARRECOMBINANT & NATURAL PROTEINS


EDAR/Fc Chimera Human Lyophilized without a carrier protein None NS0 157-ER-100 100 µg

EDAR/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 745-ED-050 50 µg

ANTIBODIES


EDAR Human None Polyclonal Goat IgG B/N, WB AF157 100 µg

EDAR Human Biotin Polyclonal Goat IgG WB BAF157 50 µg

EDAR Mouse None Polyclonal Goat IgG B/N, WB AF745 100 µg

EDAR Mouse Biotin Polyclonal Goat IgG WB BAF745 50 µg

EDAR Mouse None 132102 Rat IgG2A WB MAB745 500 µg



EDAR Mouse 312.5 - 20000 pg/mL DY745 15 Plates


Fas/TNFRSF6RECOMBINANT & NATURAL PROTEINS


Fas/TNFRSF6/Fc Chimera Feline Lyophilized without a carrier protein None NS0 2267-FA-050 50 µg

Fas/TNFRSF6/Fc Chimera Human Lyophilized with a carrier protein None NS0 326-FS-050 50 µg

Fas/TNFRSF6/Fc Chimera Mouse Lyophilized with a carrier protein None Sf 21 435-FA-050 50 µg

Fas/TNFRSF6/Fc Chimera Rat Lyophilized without a carrier protein None NS0 2159-FA-050 50 µg




Fas/TNFRSF6 continuedANTIBODIES


Fas/TNFRSF6 Feline None Polyclonal Goat IgG FC, IHC, WB AF2267 100 µg

Fas/TNFRSF6 Feline Biotin Polyclonal Goat IgG FC, IHC, WB BAF2267 50 µg

Fas/TNFRSF6 Feline None 431014 Mouse IgG1 FC, WB MAB2267 100 µg

Fas/TNFRSF6aa 25-172

Feline None 431006 Mouse IgG1 WB MAB22671 100 µg

Fas/TNFRSF6 Human None Polyclonal Goat IgG WB AF326 100 µg

Fas/TNFRSF6 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF326 50 µg

Fas/TNFRSF6 Human Fluorescein DX2 Mouse IgG1 FC FAB142F 100 Tests

Fas/TNFRSF6 Human None DX2 Mouse IgG1 IHC, WB MAB142 500 µg

Fas/TNFRSF6 Human None 50830 Mouse IgG1 ELISA(Cap) MAB144 500 µg

Fas/TNFRSF6 Mouse None Polyclonal Goat IgG ELISA(Cap), FC, IHC,WB

AF435 100 µg

Fas/TNFRSF6 Mouse Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF435 50 µg

Fas/TNFRSF6 Mouse None 169732 Rat IgG2B FC MAB435 500 µg

Fas/TNFRSF6 Rat None Polyclonal Goat IgG FC, IHC, WB AF2159 100 µg

Fas/TNFRSF6 Rat Biotin Polyclonal Goat IgG WB BAF2159 50 µg

ELISAs/ASSAYS


Fas/TNFRSF6 Human � Serum

� Citrate, EDTA, andHeparin Plasma


20 pg/mL 31.2-2000 pg/mL DFS00 1 Kit



Fas/TNFRSF6 Human 62.5 - 4000 pg/mL DY326 15 Plates

Fas/TNFRSF6 Mouse 62.5 - 4000 pg/mL DY435 15 Plates


PRIMER PAIRS


Fas/TNFRSF6 Human/Mouse RDP-59-025 25 Tests




TNF Superfamily

Fas Ligand/TNFSF6RECOMBINANT & NATURAL PROTEINS


Fas Ligand/TNFSF6 Human Lyophilized with a carrier protein None CHO 126-FL-010 10 µg

Fas Ligand/TNFSF6 Mouse Lyophilized with a carrier protein None NS0 526-SA-050 50 µg

Fas Ligand/TNFSF6 Rat Lyophilized with a carrier protein None NS0 1614-FL-025 25 µg

ANTIBODIES


Fas Ligand/TNFSF6 Human None Polyclonal Total Goat IgG B/N, WB AB126 1 mg

Fas Ligand/TNFSF6 Human None Polyclonal Goat IgG B/N, FC, IHC, WB AF126 100 µg

Fas Ligand/TNFSF6 Human Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF126 50 µg

Fas Ligand/TNFSF6 Human None 100419 Mouse IgG2B B/N, ELISA(Cap), FC MAB126 500 µg

Fas Ligand/TNFSF6 Human None 154922 Mouse IgG1 WB MAB1261 500 µg

Fas Ligand/TNFSF6 Mouse None Polyclonal Goat IgG B/N, WB AF526 100 µg

Fas Ligand/TNFSF6 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF526 50 µg

Fas Ligand/TNFSF6 Mouse None 101624 Rat IgG1 B/N, WB MAB526 500 µg

Fas Ligand/TNFSF6 Mouse None 101626 Rat IgG1 B/N, ELISA(Cap), WB MAB5262 500 µg

Fas Ligand/TNFSF6 Rat None Polyclonal Goat IgG IHC, WB AF1858 100 µg

Fas Ligand/TNFSF6 Rat Biotin Polyclonal Goat IgG WB BAF1889 50 µg

Fas Ligand/TNFSF6 Rat None 252029 Mouse IgG2B IHC, WB MAB1858 500 µg

ELISAs/ASSAYS


Fas Ligand/TNFSF6 Human � Serum



� Cell Lysate

8.05 pg/mL 15.6-1000 pg/mL DFL00 1 Kit

Fas Ligand/TNFSF6 Mouse � Serum

� EDTA Plasma


3.9 pg/mL 31.2-2000 pg/mL MFL00 1 Kit



Fas Ligand/TNFSF6 Human 31.2 - 2000 pg/mL DY126 15 Plates

Fas Ligand/TNFSF6 Mouse 15.6 - 1000 pg/mL DY526 15 Plates


PRIMER PAIRS


Fas Ligand/TNFSF6 Human/Mouse RDP-58-025 25 Tests





GITR/TNFRSF18RECOMBINANT & NATURAL PROTEINS


GITR/TNFRSF18/Fc Chimera Human Lyophilized without a carrier protein None NS0 689-GR-100 100 µg

GITR/TNFRSF18/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 524-GR-050 50 µg

ANTIBODIES


GITR/TNFRSF18 Human None Polyclonal Goat IgG B/N, FC, IHC, WB AF689 100 µg

GITR/TNFRSF18 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF689 50 µg

GITR/TNFRSF18 Human APC 110416 Mouse IgG1 FC FAB689A 100 Tests

GITR/TNFRSF18 Human Fluorescein 110416 Mouse IgG1 FC FAB689F 100 Tests

GITR/TNFRSF18 Human PE 110416 Mouse IgG1 FC FAB689P 100 Tests

GITR/TNFRSF18 Human None 110416 Mouse IgG1 B/N, ELISA(Cap), FC,WB

MAB689 500 µg

GITR/TNFRSF18 Mouse None Polyclonal Goat IgG WB AF524 100 µg

GITR/TNFRSF18 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF524 50 µg

GITR/TNFRSF18 Mouse Fluorescein 108619 Rat IgG2A FC FAB5241F 100 Tests

GITR/TNFRSF18 Mouse PE 108619 Rat IgG2A FC FAB5241P 100 Tests

GITR/TNFRSF18 Mouse None 108626 Rat IgG1 ELISA(Cap), WB MAB524 500 µg

GITR/TNFRSF18 Mouse None 108619 Rat IgG2A FC, WB MAB5241 500 µg



GITR/TNFRSF18 Human 62.5 - 4000 pg/mL DY689 15 Plates

GITR/TNFRSF18 Mouse 62.5 - 4000 pg/mL DY524 15 Plates


PRIMER PAIRS


GITR/TNFRSF18 Human RDP-128-025 25 Tests

GITR Ligand/TNFSF18RECOMBINANT & NATURAL PROTEINS


GITR Ligand/TNFSF18 Human Lyophilized with a carrier protein None Sf 21 694-GL-025 25 µg

GITR Ligand/TNFSF18 Mouse Lyophilized with a carrier protein None NS0 2177-GL-025 25 µg

ANTIBODIES


GITR Ligand/TNFSF18 Human None Polyclonal Goat IgG B/N, FC, WB AF694 100 µg

GITR Ligand/TNFSF18 Human Biotin Polyclonal Goat IgG FC, WB BAF694 50 µg

GITR Ligand/TNFSF18 Human Biotin 109117 Mouse IgG1 ELISA(Det) BAM6943 250 µg

GITR Ligand/TNFSF18 Human APC 109101 Mouse IgG1 FC FAB6941A 100 Tests

GITR Ligand/TNFSF18 Human Fluorescein 109101 Mouse IgG1 FC FAB6941F 100 Tests

GITR Ligand/TNFSF18 Human PE 109101 Mouse IgG1 FC FAB6941P 100 Tests

GITR Ligand/TNFSF18 Human None 109101 Mouse IgG1 B/N, FC MAB6941 500 µg

GITR Ligand/TNFSF18 Human None 109114 Mouse IgG1 B/N, ELISA(Cap), FC MAB6942 500 µg



TNF Superfamily

GITR Ligand/TNFSF18 continuedANTIBODIES


GITR Ligand/TNFSF18 Mouse None Polyclonal Goat IgG WB AF2177 100 µg

GITR Ligand/TNFSF18 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF2177 50 µg

GITR Ligand/TNFSF18 Mouse None 337122 Rat IgG2A B/N, ELISA(Cap), WB MAB2177 500 µg

GITR Ligand/TNFSF18 Mouse None 337112 Rat IgG2A WB MAB21771 100 µg



GITR Ligand/TNFSF18 Human 125 - 8000 pg/mL DY694 15 Plates

GITR Ligand/TNFSF18 Mouse 31.25 - 2000 pg/mL DY2177 15 Plates


HVEM/TNFRSF14RECOMBINANT & NATURAL PROTEINS


HVEM/TNFRSF14/Fc Chimera Human Lyophilized with a carrier protein None NS0 356-HV-100 100 µg

ANTIBODIES


HVEM/TNFRSF14 Human None Polyclonal Goat IgG WB AF356 100 µg

HVEM/TNFRSF14 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF356 50 µg

HVEM/TNFRSF14 Human APC 94801 Mouse IgG1 FC FAB356A 100 Tests

HVEM/TNFRSF14 Human None 94801 Mouse IgG1 ELISA(Cap), FC, WB MAB356 500 µg

HVEM/TNFRSF14 Human None 94804 Mouse IgG1 IHC, WB MAB3561 500 µg

HVEM/TNFRSF14 Mouse None Polyclonal Goat IgG WB AF2516 100 µg



HVEM/TNFRSF14 Human 15.625 - 1000 pg/mL DY356 15 Plates


LIGHT/TNFSF14RECOMBINANT & NATURAL PROTEINS


LIGHT/TNFSF14 Human Lyophilized with a carrier protein None NS0 664-LI-025 25 µg

LIGHT/TNFSF14 Mouse Lyophilized with a carrier protein None NS0 1794-LT-025 25 µg

ANTIBODIES


LIGHT/TNFSF14 Human None Polyclonal Goat IgG B/N, WB AF664 100 µg

LIGHT/TNFSF14 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF664 50 µg

LIGHT/TNFSF14 Human APC 115520 Mouse IgG1 FC FAB664A 100 Tests

LIGHT/TNFSF14 Human PE 115520 Mouse IgG1 FC FAB664P 100 Tests

LIGHT/TNFSF14 Human None 115520 Mouse IgG1 ELISA(Cap), FC MAB664 500 µg

LIGHT/TNFSF14 Mouse None Polyclonal Goat IgG WB AF1794 100 µg

LIGHT/TNFSF14 Mouse Biotin Polyclonal Goat IgG WB BAF1794 50 µg

LIGHT/TNFSF14 Mouse None 261639 Rat IgG2A WB MAB1794 100 µg




LIGHT/TNFSF14 continuedELISAs/ASSAYS


LIGHT/TNFSF14 Human � Serum



� Saliva

16.5 pg/mL 31.2-2000 pg/mL DLIT00 1 Kit



LIGHT/TNFSF14 Human 62.5 - 4000 pg/mL DY664 15 Plates


PRIMER PAIRS


LIGHT/TNFSF14 Human RDP-122-025 25 Tests

Lymphotoxin �

RECOMBINANT & NATURAL PROTEINS


Lymphotoxin �1/�2 Human Lyophilized with a carrier protein None Sf 21 678-LY-010 10 µg

Lymphotoxin �2/�1 Human Lyophilized with a carrier protein None Sf 21 679-TX-010 10 µg

Lymphotoxin �2/�1 Mouse Lyophilized with a carrier protein None Sf 21 1008-LY-010 10 µg

ANTIBODIES


Lymphotoxin �/� Human None 135125 Mouse IgG1 B/N MAB1370 500 µg

Lymphotoxin �/TNFSF3ANTIBODIES


Lymphotoxin �/TNFSF3 Human None 135105 Mouse IgG2B WB MAB1684 500 µg

Lymphotoxin � R/TNFRSF3RECOMBINANT & NATURAL PROTEINS


Lymphotoxin � R/TNFRSF3/Fc Chimera

Human Lyophilized without a carrier protein None NS0 629-LR-100 100 µg

ANTIBODIES


Lymphotoxin � R/TNFRSF3 Human None Polyclonal Goat IgG FA, FC, WB AF629 100 µg

Lymphotoxin � R/TNFRSF3 Human Biotin Polyclonal Goat IgG FC, WB BAF629 50 µg

Lymphotoxin � R/TNFRSF3 Human APC 71319 Mouse IgG1 FC FAB629A 100 Tests

Lymphotoxin � R/TNFRSF3 Human PE 71319 Mouse IgG1 FC FAB629P 100 Tests

Lymphotoxin � R/TNFRSF3 Human None 71319 Mouse IgG1 FC, WB MAB629 500 µg

Lymphotoxin � R/TNFRSF3 Mouse None Polyclonal Goat IgG FC, IHC, WB AF1008 100 µg

Lymphotoxin � R/TNFRSF3 Mouse Biotin Polyclonal Goat IgG FC, IHC, WB BAF1008 50 µg

Lymphotoxin � R/TNFRSF3 Mouse None 157105 Rat IgG2B WB MAB1008 500 µg

Lymphotoxin � R/TNFRSF3 Mouse None 157108 Rat IgG2A FC, WB MAB10081 500 µg




TNF Superfamily

NGF R/TNFRSF16RECOMBINANT & NATURAL PROTEINS


NGF R/TNFRSF16/Fc Chimera

Human Lyophilized with a carrier protein None Sf 21 367-NR-050 50 µg

NGF R/TNFRSF16/Fc Chimera

Mouse Lyophilized without a carrier protein None NS0 1157-NR-050 50 µg

ANTIBODIES


NGF R/TNFRSF16 Human None Polyclonal Goat IgG WB AF367 100 µg

NGF R/TNFRSF16 Human Biotin Polyclonal Goat IgG WB BAF367 50 µg

NGF R/TNFRSF16 Human None 74902 Mouse IgG1 IHC, WB MAB367 100 µg

NGF R/TNFRSF16 Mouse None Polyclonal Goat IgG IHC, WB AF1157 100 µg

NGF R/TNFRSF16 Mouse Biotin Polyclonal Goat IgG IHC, WB BAF1157 50 µg

PRIMER PAIRS


NGF R/TNFRSF16 Human RDP-112-025 25 Tests

NGF R/TNFRSF16 Mouse/Rat RDP-113-025 25 Tests

Osteoprotegerin/TNFRSF11BRECOMBINANT & NATURAL PROTEINS


Osteoprotegerin/TNFRSF11B

Human Lyophilized with a carrier protein None NS0 185-OS-025 25 µg

Osteoprotegerin/TNFRSF11B/Fc Chimera

Human Lyophilized with a carrier protein None Sf 21 805-OS-100 100 µg

Osteoprotegerin/TNFRSF11B/Fc Chimera

Mouse Lyophilized without a carrier protein None NS0 459-MO-100 100 µg

ANTIBODIES



Human None Polyclonal Goat IgG B/N, IHC, WB AF805 100 µg


Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF805 50 µg


Human None 69146 Mouse IgG1 B/N MAB805 500 µg


Human None 69127 Mouse IgG2A ELISA(Cap) MAB8051 500 µg


Mouse None Polyclonal Goat IgG B/N, WB AF459 100 µg


Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF459 50 µg


Mouse None 110517 Rat IgG2A WB MAB459 500 µg


Mouse None 110511 Rat IgG2A ELISA(Cap), WB MAB4591 500 µg





Osteoprotegerin/TNFRSF11B continuedELISAs/ASSAYS



Mouse � Serum

� EDTA Plasma


4.5 pg/mL 31.2-2000 pg/mL MOP00 1 Kit




Human 62.5 - 4000 pg/mL DY805 15 Plates


Mouse 62.5 - 4000 pg/mL DY459 15 Plates


PRIMER PAIRS


Osteoprotegerin/TNFRSF11B Human/Mouse RDP-87-025 25 Tests

OX40/TNFRSF4RECOMBINANT & NATURAL PROTEINS


OX40/TNFRSF4/Fc Chimera Human Lyophilized without a carrier protein None NS0 3388-OX-050 50 µg

OX40/TNFRSF4/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 1256-OX-050 50 µg

ANTIBODIES


OX40/TNFRSF4 Human None Polyclonal Sheep IgG FC, WB AF3388 100 µg

OX40/TNFRSF4 Human Biotin Polyclonal Sheep IgG FC, WB BAF3388 50 µg

OX40/TNFRSF4 Human None 443318 Rat IgG2A FC, WB MAB3388 100 µg

OX40/TNFRSF4 Mouse None Polyclonal Goat IgG FA, WB AF1256 100 µg

OX40/TNFRSF4 Mouse Biotin Polyclonal Goat IgG WB BAF1256 50 µg

OX40 Ligand/TNFSF4RECOMBINANT & NATURAL PROTEINS


OX40 Ligand/TNFSF4 Human Lyophilized without a carrier protein None NS0 1054-OX-010 10 µg

OX40 Ligand/TNFSF4 Mouse Lyophilized with a carrier protein None NS0 1236-OX-025 25 µg

ANTIBODIES


OX40 Ligand/TNFSF4 Human None Polyclonal Goat IgG B/N, FC, WB AF1054 100 µg

OX40 Ligand/TNFSF4 Human Biotin Polyclonal Goat IgG WB BAF1054 50 µg

OX40 Ligand/TNFSF4 Human None 159408 Mouse IgG1 WB MAB1054 500 µg

OX40 Ligand/TNFSF4 Human None 159403 Mouse IgG1 B/N, FC, IHC MAB10541 500 µg

OX40 Ligand/TNFSF4 Mouse None Polyclonal Goat IgG B/N, IHC, WB AF1236 100 µg

OX40 Ligand/TNFSF4 Mouse Biotin Polyclonal Goat IgG WB BAF1236 50 µg

OX40 Ligand/TNFSF4 Mouse None 182601 Rat IgG2A B/N, WB MAB1236 500 µg



TNF Superfamily

RANK/TNFRSF11ARECOMBINANT & NATURAL PROTEINS


RANK/TNFRSF11A/Fc Chimera Human Lyophilized without a carrier protein None NS0 683-RK-100 100 µg

RANK/TNFRSF11A/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 692-RK-100 100 µg

ANTIBODIES


RANK/TNFRSF11A Human None Polyclonal Goat IgG FA, FC, WB AF683 100 µg

RANK/TNFRSF11A Human Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF683 50 µg

RANK/TNFRSF11A Human None 80704 Mouse IgG1 ELISA(Cap), FC, WB MAB683 500 µg

RANK/TNFRSF11A Human None 80707 Mouse IgG2A IHC, WB MAB6831 500 µg

RANK/TNFRSF11A Mouse None Polyclonal Goat IgG FA, IHC, WB AF692 100 µg

RANK/TNFRSF11A Mouse Biotin Polyclonal Goat IgG WB BAF692 50 µg

RANK/TNFRSF11A Mouse None 100024 Rat IgG2A WB MAB692 500 µg



RANK/TNFRSF11A Human 62.5 - 4000 pg/mL DY683 15 Plates


PRIMER PAIRS


RANK/TNFRSF11A Human RDP-67-025 25 Tests

RANK/TNFRSF11A Mouse RDP-72-025 25 Tests

RANK Ligand, see page 55.

RELT/TNFRSF19LRECOMBINANT & NATURAL PROTEINS


RELT/TNFRSF19L/Fc Chimera Human Solution without a carrier protein None NS0 1385-RT-050 50 µg

ANTIBODIES


RELT/TNFRSF19L Human None Polyclonal Goat IgG WB AF1385 100 µg

RELT/TNFRSF19L Human Biotin Polyclonal Goat IgG WB BAF1385 50 µg

RELT/TNFRSF19L Human None 238104 Mouse IgG2B FC MAB1385 500 µg





TACI/TNFRSF13BRECOMBINANT & NATURAL PROTEINS


TACI/TNFRSF13B/Fc Chimera

Human Lyophilized without a carrier protein None NS0 174-TC-050 50 µg

TACI/TNFRSF13B/Fc Chimera

Mouse Lyophilized without a carrier protein None NS0 1041-TC-050 50 µg

ANTIBODIES


TACI/TNFRSF13B Human None Polyclonal Goat IgG B/N, IHC, WB AF174 100 µg

TACI/TNFRSF13B Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF174 50 µg

TACI/TNFRSF13B Human APC 165604 Mouse IgG1 FC FAB1741A 100 Tests

TACI/TNFRSF13B Human PE 165604 Mouse IgG1 FC FAB1741P 100 Tests

TACI/TNFRSF13B Human None 165609 Mouse IgG1 B/N, IHC, WB MAB174 500 µg

TACI/TNFRSF13B Human None 165604 Mouse IgG1 B/N, FC MAB1741 500 µg

TACI/TNFRSF13B Mouse None Polyclonal Goat IgG B/N, FC, IHC, WB AF1041 100 µg

TACI/TNFRSF13B Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1041 50 µg

TACI/TNFRSF13B Mouse PE 166010 Rat IgG2A FC FAB1041P 100 Tests

TACI/TNFRSF13B Mouse None 166010 Rat IgG2A ELISA(Cap), FC, WB MAB1041 500 µg



TACI/TNFRSF13B Human 93.8 - 6000 pg/mL DY174 15 Plates

TACI/TNFRSF13B Mouse 46.9 - 3000 pg/mL DY1041 15 Plates


PRIMER PAIRS


TACI/TNFRSF13B Human RDP-142-025 25 Tests

TL1A/TNFSF15RECOMBINANT & NATURAL PROTEINS


TL1A/TNFSF15 Human Lyophilized with a carrier protein None E. coli 1319-TL-010 10 µg

TL1A/TNFSF15 Mouse Lyophilized with a carrier protein None E. coli 1896-TL-010 10 µg

ANTIBODIES


TL1A/TNFSF15 Human None Polyclonal Goat IgG WB AF744 100 µg

TL1A/TNFSF15 Human Biotin Polyclonal Goat IgG WB BAF744 50 µg

TL1A/TNFSF15 Human/Mouse None 293327 Rat IgG1 WB MAB7441 100 µg



TNF Superfamily

TNF-�/TNFSF1ARECOMBINANT & NATURAL PROTEINS


TNF-�/TNFSF1A Bovine Lyophilized with a carrier protein None E. coli 2279-BT-025 25 µg

TNF-�/TNFSF1A Canine Lyophilized with a carrier protein None E. coli 1507-CT-025 25 µg

TNF-�/TNFSF1A Cotton Rat Lyophilized with a carrier protein None E. coli 1011-CR-025 25 µg

TNF-�/TNFSF1A Equine Lyophilized with a carrier protein None E. coli 1814-ET-025 25 µg

TNF-�/TNFSF1A Feline Lyophilized with a carrier protein None E. coli 2586-FT-025 25 µg

TNF-�/TNFSF1A Human Lyophilized with a carrier protein None E. coli 210-TA-010 10 µg

TNF-�/TNFSF1A Human Lyophilized with a carrier protein None E. coli 210-TA-050 50 µg

TNF-�/TNFSF1APro Form

Human Lyophilized without a carrier protein None E. coli 1012-PS-010 10 µg

TNF-�/TNFSF1A Mouse Lyophilized with a carrier protein None E. coli 410-MT-010 10 µg

TNF-�/TNFSF1A Mouse Lyophilized with a carrier protein None E. coli 410-MT-050 50 µg

TNF-�/TNFSF1AN-Terminal Truncated Form

Mouse Lyophilized with a carrier protein None E. coli 410-TRNC-010 10 µg

TNF-�/TNFSF1AN-Terminal Truncated Form

Mouse Lyophilized with a carrier protein None E. coli 410-TRNC-050 50 µg

TNF-�/TNFSF1A Porcine Lyophilized with a carrier protein None E. coli 690-PT-025 25 µg

TNF-�/TNFSF1A Primate Lyophilized with a carrier protein None E. coli 1070-RM-025 25 µg

TNF-�/TNFSF1A Rat Lyophilized with a carrier protein None E. coli 510-RT-010 10 µg

TNF-�/TNFSF1A Rat Lyophilized with a carrier protein None E. coli 510-RT-050 50 µg

ANTIBODIES


TNF-�/TNFSF1A Bovine None Polyclonal Goat IgG IHC, WB AF2279 100 µg

TNF-�/TNFSF1A Bovine Biotin Polyclonal Goat IgG IHC, WB BAF2279 50 µg

TNF-�/TNFSF1A Canine None Polyclonal Goat IgG B/N, IHC, WB AF1507 100 µg

TNF-�/TNFSF1A Canine Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1507 50 µg

TNF-�/TNFSF1A Canine None 236812 Mouse IgG1 B/N, ELISA(Cap), IHC MAB1507 500 µg

TNF-�/TNFSF1A Cotton Rat None Polyclonal Goat IgG B/N, WB AF1011 100 µg

TNF-�/TNFSF1A Cotton Rat Biotin Polyclonal Goat IgG WB BAF1011 50 µg

TNF-�/TNFSF1A Cotton Rat None 159811 Mouse IgG2B B/N, WB MAB1011 500 µg

TNF-�/TNFSF1A Cotton Rat None 159813 Mouse IgG1 B/N, WB MAB10111 500 µg

TNF-�/TNFSF1A Equine None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,WB

AF1814 100 µg

TNF-�/TNFSF1A Human None Polyclonal Goat IgG B/N, IHC, WB AF-210-NA 100 µg

TNF-�/TNFSF1A Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF210 50 µg

TNF-�/TNFSF1A Human Fluorescein 6402 Mouse IgG1 FC IC210F 100 Tests

TNF-�/TNFSF1A Human PE 6402 Mouse IgG1 FC IC210P 100 Tests

TNF-�/TNFSF1A Human None 1825 Mouse IgG1 B/N MAB210 500 µg

TNF-�/TNFSF1A Human None 6401 Mouse IgG1 B/N, FC MAB2101 500 µg

TNF-�/TNFSF1A Human None 28401 Mouse IgG1 B/N, ELISA(Cap), IHC,WB

MAB610 500 µg

TNF-�/TNFSF1AMembrane Form

Human Fluorescein 6401 Mouse IgG1 FC FAB210F 50 Tests

TNF-�/TNFSF1A Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), FC,IHC, WB

AF-410-NA 100 µg

TNF-�/TNFSF1A Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF410 50 µg

TNF-�/TNFSF1A Mouse Fluorescein MP6-XT22 Rat IgG1 FC IC410F 100 Tests

TNF-�/TNFSF1A Mouse PE MP6-XT22 Rat IgG1 FC IC410P 100 Tests

TNF-�/TNFSF1A Mouse None TN319 Hamster IgG WB MAB410 500 µg

TNF-�/TNFSF1A Mouse None MP6-XT22 Rat IgG1 B/N MAB4101 100 µg





TNF-�/TNFSF1A continuedANTIBODIES


TNF-�/TNFSF1A Porcine None Polyclonal Goat IgG B/N, IHC, WB AF690 100 µg

TNF-�/TNFSF1A Porcine Biotin Polyclonal Goat IgG ELISA(Det), WB BAF690 50 µg

TNF-�/TNFSF1A Porcine None 77938 Mouse IgG1 WB MAB690 500 µg

TNF-�/TNFSF1A Porcine None 103314 Mouse IgG1 B/N MAB6901 500 µg

TNF-�/TNFSF1A Porcine None 103304 Mouse IgG1 ELISA(Cap) MAB6902 500 µg

TNF-�/TNFSF1A Porcine None 103302 Mouse IgG1 B/N, IHC, WB MAB6903 100 µg

TNF-�/TNFSF1A Primate None Polyclonal Goat IgG WB AF1070 100 µg

TNF-�/TNFSF1A Primate Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1070 50 µg

TNF-�/TNFSF1A Primate None 182303 Rat IgG2B WB MAB1070 500 µg

TNF-�/TNFSF1A Primate None 182309 Rat IgG1 B/N MAB10701 500 µg

TNF-�/TNFSF1A Primate None 182312 Rat IgG2A ELISA(Cap) MAB10702 500 µg

TNF-�/TNFSF1A Rat None Polyclonal Goat IgG B/N, IHC, WB AF-510-NA 100 µg

TNF-�/TNFSF1A Rat Biotin Polyclonal Goat IgG ELISA(Det), WB BAF510 50 µg

TNF-�/TNFSF1A Rat None 45418 Mouse IgG1 B/N, ELISA(Cap), WB MAB510 500 µg

ELISAs/ASSAYS


TNF-�/TNFSF1A Canine � Serum



4.2 pg/mL 7.8-500 pg/mL CATA00 1 Kit

TNF-�/TNFSF1A Human � Serum



5.5 pg/mL 15.6-1000 pg/mL DTA00C 1 Kit

TNF-�/TNFSF1AChemiluminescent

Human � Serum



0.736 pg/mL 2.2-7000 pg/mL QTA00B 1 Kit

TNF-�/TNFSF1AHigh Sensitivity

Human � Serum


0.191 pg/mL 0.5-32 pg/mL HSTA00D 1 Kit

TNF-�/TNFSF1A2 Plate

Mouse � Serum


5.1 pg/mL 23.4-1500 pg/mL MTA00 1 Kit

TNF-�/TNFSF1A Porcine � Serum



5 pg/mL 23.4-1500 pg/mL PTA00 1 Kit

TNF-�/TNFSF1A Primate � Serum

� EDTA Plasma


6.2 pg/mL 15.6-1000 pg/mL RHMTA0 1 Kit

TNF-�/TNFSF1A2 Plate

Rat � Serum



5 pg/mL 12.5-800 pg/mL RTA00 1 Kit



TNF Superfamily

TNF-�/TNFSF1A continuedDUOSET ELISA DEVELOPMENT SYSTEMS


TNF-�/TNFSF1A Canine 15.6 - 1000 pg/mL DY1507 15 Plates

TNF-�/TNFSF1A Equine 31.2 - 2000 pg/mL DY1814 15 Plates

TNF-�/TNFSF1A Feline 15.625 - 1000 pg/mL DY2586 15 Plates

TNF-�/TNFSF1A Human 15.6 - 1000 pg/mL DY210 15 Plates

TNF-�/TNFSF1A Mouse 31.2 - 2000 pg/mL DY410 15 Plates

TNF-�/TNFSF1A Porcine 125 - 8000 pg/mL DY690 15 Plates

TNF-�/TNFSF1A Primate 31.2 - 2000 pg/mL DY1070 15 Plates

TNF-�/TNFSF1A Rat 62.5 - 4000 pg/mL DY510 15 Plates


ELISPOT KITS

ANALYTE SPECIES CATALOG # SIZE**

TNF-�/TNFSF1A Human EL210 1 Kit

TNF-�/TNFSF1A Mouse EL410 1 Kit

TNF-�/TNFSF1A Porcine EL690 1 Kit

TNF-�/TNFSF1A Primate EL1070 1 Kit

** ELISpot Kits are also available in 6 and 50 plate packs and development modules containing enough reagents for 5 plates, please inquire.

PRIMER PAIRS


TNF-�/TNFSF1A Human RDP-10-025 25 Tests

TNF-�/TNFSF1A Mouse RDP-60-025 25 Tests

TNF-�/TNFSF1BRECOMBINANT & NATURAL PROTEINS


TNF-�/TNFSF1B Human Lyophilized with a carrier protein None E. coli 211-TB-010 10 µg

TNF-�/TNFSF1B Human Lyophilized with a carrier protein None E. coli 211-TB-050 50 µg

TNF-�/TNFSF1B Mouse Lyophilized with a carrier protein None NS0 749-TB-025 25 µg

ANTIBODIES


TNF-�/TNFSF1B Human None Polyclonal Goat IgG B/N, WB AF-211-NA 100 µg

TNF-�/TNFSF1B Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF211 50 µg

TNF-�/TNFSF1B Human Fluorescein 5808 Mouse IgG1 FC IC2111F 100 Tests

TNF-�/TNFSF1B Human PE 5808 Mouse IgG1 FC IC2111P 100 Tests

TNF-�/TNFSF1B Human None 5802 Mouse IgG1 IHC MAB211 500 µg

TNF-�/TNFSF1B Human None 5807 Mouse IgG2A B/N, ELISA(Cap) MAB621 1 mg

TNF-�/TNFSF1B Mouse None Polyclonal Goat IgG WB AF749 100 µg

TNF-�/TNFSF1B Mouse Biotin Polyclonal Goat IgG IHC, WB BAF749 50 µg

TNF-�/TNFSF1B Mouse None 168717 Rat IgG2B WB MAB749 500 µg

ELISAs/ASSAYS


TNF-�/TNFSF1B Human � Cell Culture Supernate 7 pg/mL 31.2-2000 pg/mL DTB00 1 Kit




TNF-�/TNFSF1B continuedDUOSET ELISA DEVELOPMENT SYSTEMS


TNF-�/TNFSF1B Human 31.2 - 2000 pg/mL DY211 15 Plates


PRIMER PAIRS


TNF-�/TNFSF1B Human RDP-22-025 25 Tests

TNF RI/TNFRSF1ARECOMBINANT & NATURAL PROTEINS


TNF RI/TNFRSF1A Canine Lyophilized with a carrier protein None E. coli 4017-TR-025 25 µg

TNF RI/TNFRSF1A Human Lyophilized with a carrier protein None E. coli 636-R1-025 25 µg

TNF RI/TNFRSF1A/Fc Chimera

Human Lyophilized with a carrier protein None NS0 372-RI-050 50 µg

TNF RI/TNFRSF1A Mouse Lyophilized with a carrier protein None E. coli 425-R1-050 50 µg

TNF RI/TNFRSF1A/Fc Chimera

Mouse Lyophilized with a carrier protein None NS0 430-RI-050 50 µg

ANTIBODIES


TNF RI/TNFRSF1A Human None Polyclonal Goat IgG FA, FC, IHC, WB AF225 100 µg

TNF RI/TNFRSF1A Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF225 50 µg

TNF RI/TNFRSF1A Human Fluorescein 16803 Mouse IgG1 FC FAB225F 100 Tests

TNF RI/TNFRSF1A Human PE 16803 Mouse IgG1 FC FAB225P 100 Tests

TNF RI/TNFRSF1A Human None 16803 Mouse IgG1 B/N, FC, WB MAB225 500 µg

TNF RI/TNFRSF1A Human None 16805 Mouse IgG1 B/N, ELISA(Cap), WB MAB625 500 µg

TNF RI/TNFRSF1A Mouse None Polyclonal Goat IgG IHC, WB AF-425-PB 100 µg

TNF RI/TNFRSF1A Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF425 50 µg

TNF RI/TNFRSF1A Mouse None 47803 Rat IgG1 ELISA(Cap), WB MAB425 500 µg

TNF RI/TNFRSF1A Mouse None 55R170 Hamster IgG B/N, IP, WB MAB430 500 µg

ELISAs/ASSAYS


TNF RI/TNFRSF1A Human � Serum



� Urine

1.2 pg/mL 7.8-500 pg/mL DRT100 1 Kit

TNF RI/TNFRSF1A Mouse � Serum


5 pg/mL 15.6-1000 pg/mL MRT10 1 Kit



TNF RI/TNFRSF1A Human 12.5 - 800 pg/mL DY225 15 Plates

TNF RI/TNFRSF1A Mouse 7.81 - 500 pg/mL DY425 15 Plates





TNF Superfamily

TNF RI/TNFRSF1A continuedPRIMER PAIRS


TNF RI/TNFRSF1A Human RDP-61-025 25 Tests

TNF RI/TNFRSF1A Mouse RDP-80-025 25 Tests

TNF RII/TNFRSF1BRECOMBINANT & NATURAL PROTEINS


TNF RII/TNFRSF1Baa 24-206

Human Lyophilized with a carrier protein None E. coli 1089-R2-025 25 µg

TNF RII/TNFRSF1B/Fc Chimera

Human Lyophilized without a carrier protein None NS0 726-R2-050 50 µg

TNF RII/TNFRSF1B Mouse Lyophilized with a carrier protein None E. coli 426-R2-050 50 µg

ANTIBODIES


TNF RII/TNFRSF1B Human None Polyclonal Total Goat IgG IHC, WB AB-226-PB 1 mg

TNF RII/TNFRSF1B Human None Polyclonal Goat IgG B/N, FC, WB AF726 100 µg

TNF RII/TNFRSF1B Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF726 50 µg

TNF RII/TNFRSF1B Human Fluorescein 22235 Mouse IgG2A FC FAB226F 100 Tests

TNF RII/TNFRSF1B Human PE 22235 Mouse IgG2A FC FAB226P 100 Tests

TNF RII/TNFRSF1B Human None 22221 Mouse IgG2A B/N, IHC MAB226 500 µg

TNF RII/TNFRSF1B Human None 22235 Mouse IgG2A FC MAB2261 500 µg

TNF RII/TNFRSF1B Human None 22210 Mouse IgG1 B/N, ELISA(Cap), WB MAB726 500 µg

TNF RII/TNFRSF1B Mouse None Polyclonal Goat IgG IHC, WB AF-426-PB 100 µg

TNF RII/TNFRSF1B Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF426 50 µg

TNF RII/TNFRSF1B Mouse None TR7554 Hamster IgG ELISA(Cap), WB MAB426 500 µg

TNF RII/TNFRSF1B Mouse None TR7532 Hamster IgG WB MAB4261 500 µg

TNF RII/TNFRSF1B Mouse None TR75-89.29 Hamster IgG IP, WB MAB4262 500 µg

ELISAs/ASSAYS


TNF RII/TNFRSF1B Human � Serum



� Urine

2.3 pg/mL 7.8-500 pg/mL DRT200 1 Kit

TNF RII/TNFRSF1B Mouse � Serum

� EDTA Plasma


5 pg/mL 15.6-1000 pg/mL MRT20 1 Kit



TNF RII/TNFRSF1B Human 7.81 - 500 pg/mL DY726 15 Plates

TNF RII/TNFRSF1B Mouse 7.81 - 500 pg/mL DY426 15 Plates


PRIMER PAIRS


TNF RII/TNFRSF1B Human/Mouse RDP-62-025 25 Tests





TRAIL/TNFSF10RECOMBINANT & NATURAL PROTEINS


TRAIL/TNFSF10 Human Lyophilized with a carrier protein None NS0 375-TL-010 10 µg

TRAIL/TNFSF10 Human Lyophilized with a carrier protein None E. coli 375-TEC-010 10 µg

TRAIL/TNFSF10 Mouse Lyophilized with a carrier protein None E. coli 1121-TL-010 10 µg

ANTIBODIES


TRAIL/TNFSF10 Human None Polyclonal Goat IgG B/N, IHC, WB AF375 100 µg

TRAIL/TNFSF10 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF375 50 µg

TRAIL/TNFSF10 Human PE 75402 Mouse IgG1 FC FAB687P 100 Tests

TRAIL/TNFSF10 Human None 75411 Mouse IgG1 B/N, IHC MAB375 500 µg

TRAIL/TNFSF10 Human None 124723 Mouse IgG1 ELISA(Cap) MAB3751 500 µg

TRAIL/TNFSF10 Human None 75402 Mouse IgG1 FC, IHC, WB MAB687 500 µg

TRAIL/TNFSF10 Mouse None Polyclonal Goat IgG IHC, WB AF1121 100 µg

TRAIL/TNFSF10 Mouse Biotin Polyclonal Goat IgG IHC, WB BAF1121 50 µg

TRAIL/TNFSF10 Mouse None 170533 Rat IgG2A WB MAB1121 500 µg

ELISAs/ASSAYS


TRAIL/TNFSF10 Human � Serum



� Saliva

� Cell Lysate

7.87 pg/mL 15.6-1000 pg/mL DTRL00 1 Kit



TRAIL/TNFSF10 Human 23.4 - 1500 pg/mL DY375 15 Plates


PRIMER PAIRS


TRAIL/TNFSF10 Human/Mouse RDP-63-025 25 Tests

TRAIL R1/TNFRSF10ARECOMBINANT & NATURAL PROTEINS


TRAIL R1/TNFRSF10A/Fc Chimera Human Lyophilized with a carrier protein None Sf 21 347-DR-100 100 µg

ANTIBODIES


TRAIL R1/TNFRSF10A Human None Polyclonal Goat IgG B/N, IHC, WB AF347 100 µg

TRAIL R1/TNFRSF10A Human Biotin Polyclonal Goat IgG WB BAF347 50 µg

TRAIL R1/TNFRSF10A Human PE 69036 Mouse IgG1 FC FAB347P 100 Tests

TRAIL R1/TNFRSF10A Human None 69036 Mouse IgG1 FC MAB347 500 µg

PRIMER PAIRS


TRAIL R1/TNFRSF10A Human RDP-64-025 25 Tests



TNF Superfamily

TRAIL R2/TNFRSF10BRECOMBINANT & NATURAL PROTEINS


TRAIL R2/TNFRSF10B/Fc Chimera

Human Lyophilized with a carrier protein None NS0 631-T2-100 100 µg

TRAIL R2/TNFRSF10B/Fc Chimera

Mouse Lyophilized without a carrier protein None Sf 21 721-DR-100 100 µg

ANTIBODIES


TRAIL R2/TNFRSF10B Human None Polyclonal Goat IgG FA, WB AF631 100 µg

TRAIL R2/TNFRSF10B Human Biotin Polyclonal Goat IgG WB BAF631 50 µg

TRAIL R2/TNFRSF10B Human PE 71908 Mouse IgG2B FC FAB6311P 100 Tests

TRAIL R2/TNFRSF10B Human None 71903 Mouse IgG1 FA MAB631 500 µg

TRAIL R2/TNFRSF10B Human None 71908 Mouse IgG2B FC MAB6311 500 µg

TRAIL R2/TNFRSF10B Mouse None Polyclonal Goat IgG B/N, FC, WB AF721 100 µg

TRAIL R2/TNFRSF10B Mouse Biotin Polyclonal Goat IgG FC, WB BAF721 50 µg

TRAIL R2/TNFRSF10B Mouse None 118929 Rat IgG2A IHC, WB MAB1540 500 µg

PRIMER PAIRS


TRAIL R2/TNFRSF10B Human RDP-68-025 25 Tests

TRAIL R3/TNFRSF10CRECOMBINANT & NATURAL PROTEINS


TRAIL R3/TNFRSF10C/Fc Chimera

Human Lyophilized with a carrier protein None NS0 630-TR-100 100 µg

ANTIBODIES


TRAIL R3/TNFRSF10C Human None Polyclonal Goat IgG B/N, IHC, WB AF630 100 µg

TRAIL R3/TNFRSF10C Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF630 50 µg

TRAIL R3/TNFRSF10C Human PE 90906 Mouse IgG1 FC FAB6302P 100 Tests

TRAIL R3/TNFRSF10C Human None 90903 Mouse IgG1 B/N MAB630 500 µg

TRAIL R3/TNFRSF10C Human None 90905 Mouse IgG1 ELISA(Cap), IHC, WB MAB6301 500 µg

TRAIL R3/TNFRSF10C Human None 90906 Mouse IgG1 FC MAB6302 500 µg



TRAIL R3/TNFRSF10C Human 62.5 - 4000 pg/mL DY630 15 Plates


PRIMER PAIRS


TRAIL R3/TNFRSF10C Human RDP-69-025 25 Tests




TRAIL R4/TNFRSF10DRECOMBINANT & NATURAL PROTEINS


TRAIL R4/TNFRSF10D/Fc Chimera

Human Lyophilized without a carrier protein None NS0 633-TR-100 100 µg

ANTIBODIES


TRAIL R4/TNFRSF10D Human None Polyclonal Goat IgG B/N, FC, IHC, WB AF633 100 µg

TRAIL R4/TNFRSF10D Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF633 50 µg

TRAIL R4/TNFRSF10D Human PE 104918 Mouse IgG1 FC FAB633P 100 Tests

TRAIL R4/TNFRSF10D Human None 104918 Mouse IgG1 B/N, ELISA(Cap), FC,WB

MAB633 500 µg



TRAIL R4/TNFRSF10D Human 93.8 - 6000 pg/mL DY633 15 Plates


PRIMER PAIRS


TRAIL R4/TNFRSF10D Human RDP-79-025 25 Tests

TRANCE/RANK L/TNFSF11RECOMBINANT & NATURAL PROTEINS


TRANCE/TNFSF11Truncated

Human Lyophilized with a carrier protein None NS0 390-TN-010 10 µg

TRANCE/TNFSF11 Mouse Lyophilized with a carrier protein None NS0 462-TR-010 10 µg

TRANCE/TNFSF11 Mouse Lyophilized with a carrier protein None E. coli 462-TEC-010 10 µg

ANTIBODIES


TRANCE/TNFSF11 Human None Polyclonal Total Goat IgG AB626 1 mg

TRANCE/TNFSF11 Human None Polyclonal Goat IgG B/N, WB AF626 100 µg

TRANCE/TNFSF11 Human Biotin Polyclonal Goat IgG WB BAF626 50 µg

TRANCE/TNFSF11 Human None 70525 Mouse IgG2B B/N, IHC MAB626 500 µg

TRANCE/TNFSF11 Human None 70513 Mouse IgG1 WB MAB6261 500 µg

TRANCE/TNFSF11 Human None 175707 Mouse IgG1 B/N MAB6262 500 µg

TRANCE/TNFSF11 Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,WB

AF462 100 µg

TRANCE/TNFSF11 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF462 50 µg

TRANCE/TNFSF11 Mouse None 88227 Rat IgG2A WB MAB462 500 µg

ELISAs/ASSAYS


TRANCE/TNFSF11 Mouse � Serum


5 pg/mL 31.2-2000 pg/mL MTR00 1 Kit



TRANCE/TNFSF11 Mouse 62.5 - 4000 pg/mL DY462 15 Plates





TNF Superfamily

TRANCE/RANK L/TNFSF11 continuedPRIMER PAIRS


TRANCE/TNFSF11 Human/Mouse RDP-66-025 25 Tests

TROY/TNFRSF19RECOMBINANT & NATURAL PROTEINS


TROY/TNFRSF19/Fc Chimera Human Lyophilized without a carrier protein None NS0 1548-TR-100 100 µg

TROY/TNFRSF19/Fc Chimera Mouse Lyophilized without a carrier protein None Sf 21 723-TR-100 100 µg

ANTIBODIES


TROY/TNFRSF19 Human None Polyclonal Goat IgG WB AF1548 100 µg

TROY/TNFRSF19 Human Biotin Polyclonal Goat IgG WB BAF1548 50 µg

TROY/TNFRSF19 Mouse None Polyclonal Goat IgG ELISA(Cap), IHC, WB AF723 100 µg

TROY/TNFRSF19 Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF723 50 µg

TROY/TNFRSF19 Mouse None 323606 Rat IgG2A WB MAB723 100 µg



TROY/TNFRSF19 Mouse 62.5 - 4000 pg/mL DY723 15 Plates


TWEAK/TNFSF12RECOMBINANT & NATURAL PROTEINS


TWEAK/TNFSF12 Human Lyophilized with a carrier protein None E. coli 1090-TW-025 25 µg

TWEAK/TNFSF12 Mouse Lyophilized with a carrier protein None E. coli 1237-TW-025 25 µg

ANTIBODIES


TWEAK/TNFSF12 Human None Polyclonal Goat IgG B/N, WB AF1090 100 µg

TWEAK/TNFSF12 Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1090 50 µg

TWEAK/TNFSF12 Human None 173830 Mouse IgG1 ELISA(Cap) MAB1090 500 µg

TWEAK/TNFSF12 Mouse None Polyclonal Goat IgG B/N, WB AF1237 100 µg

TWEAK/TNFSF12 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1237 50 µg

TWEAK/TNFSF12 Mouse None 189803 Rat IgG2A ELISA(Cap) MAB1237 500 µg





TWEAK/TNFSF12 continuedDUOSET ELISA DEVELOPMENT SYSTEMS


TWEAK/TNFSF12 Human 62.5 - 4000 pg/mL DY1090 15 Plates

TWEAK/TNFSF12 Mouse 15.6 - 1000 pg/mL DY1237 15 Plates


PRIMER PAIRS


TWEAK/TNFSF12 Mouse/Rat RDP-120-025 25 Tests

TWEAK R/TNFRSF12RECOMBINANT & NATURAL PROTEINS


TWEAK R/TNFRSF12/Fc Chimera

Human Lyophilized without a carrier protein None NS0 1199-TW-050 50 µg

TWEAK R/TNFRSF12/Fc Chimera

Mouse Lyophilized without a carrier protein None Sf 21 1610-TW-050 50 µg

ANTIBODIES


TWEAK R/TNFRSF12 Human None Polyclonal Goat IgG B/N, IHC, WB AF1199 100 µg

TWEAK R/TNFRSF12 Human Biotin Polyclonal Goat IgG WB BAF1199 50 µg

TWEAK R/TNFRSF12aa 28-79

Human None 314502 Rat IgG2A WB MAB1199 100 µg

TWEAK R/TNFRSF12 Mouse None Polyclonal Goat IgG ELISA(Cap), FC, IHC,WB

AF1610 100 µg

TWEAK R/TNFRSF12 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1610 50 µg

TWEAK R/TNFRSF12 Mouse None 314615 Rat IgG2B WB MAB1610 100 µg

TWEAK R/TNFRSF12 Mouse None 314636 Rat IgG2A FC, WB MAB16101 100 µg



TWEAK R/TNFRSF12 Mouse 312 - 20000 pg/mL DY1610 15 Plates


XEDARRECOMBINANT & NATURAL PROTEINS


XEDAR/Fc Chimera Human Lyophilized without a carrier protein None NS0 1093-XD-050 50 µg

ANTIBODIES


XEDAR Human None Polyclonal Goat IgG B/N, WB AF1093 100 µg

XEDAR Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1093 50 µg

XEDAR Human None 173111 Mouse IgG1 B/N, WB MAB1093 500 µg

XEDAR Human None 173103 Mouse IgG1 ELISA(Cap), WB MAB10931 500 µg



XEDAR Human 15.625 - 1000 pg/mL DY1093 15 Plates




TNF Superfamily

ss

Apoptosis Adaptor Proteins

Pro-Apoptotic Signaling Adaptors

CRADD/RAIDDANTIBODIES


CRADD Human/Mouse/Rat None Polyclonal Goat IgG WB AF4680 100 µg

CrkANTIBODIES


Crkaa 205-304




Apoptotic adaptor proteins play a critical role in regulating pro-

and anti-apoptotic signaling pathways following activation of the

death receptors. Adaptor proteins, such as FADD (Fas-associated

death domain) and TRADD (TNF receptor-associated death do-

main), are recruited to ligand-activated, oligomerized death

receptors to mediate apoptotic signaling pathways. Apoptotic ad-

aptors associate with the cytoplasmic portion of the death

receptors through a domain common to both known as the death

domain (DD). Following association with the ligand-bound recep-

tor, the adaptor proteins recruit Pro-caspase-8 or Pro-caspase-10

by way of their death effector domains (DED) to form the DISC

(death-inducing signaling complex). Formation of the DISC initi-

ates a caspase signaling cascade that ultimately induces apoptosis.

Other adaptor proteins mediate apoptotic signaling through dis-

tinct mechanisms. CRADD/RAIDD (caspase and RIP adaptor with a

death domain), and PIDD (p53-induced protein with a death do-

main) are two adaptor proteins that associate with Pro-caspase-2

to form the PIDDosome following DNA damage. Formation of this

complex leads to the cleavage and activation of Caspase-2. DAXX

(Fas death domain-associated-xx) and RIP1 (receptor interacting

protein 1) are two additional adaptor proteins containing a death

domain that are recruited to Fas or to TNF RI respectively, upon

ligand binding. These two proteins are involved in mediating

caspase-independent apoptotic signaling pathways. Like TNF RI

and Fas, TRAIL receptors (TRAIL R1/DR4 or TRAIL R2/DR5) and DR3

also contain intracellular death domains and can induce apoptosis

by way of adaptor-mediated Caspase-8 or Caspase-10 recruitment.

In addition to promoting apoptosis, ligand binding to TNF RI

(TNF�), or DR3 (TWEAK) can cause the intracellular adaptor pro-

teins to recruit TRAF-2 (TNF Receptor-associated factor 2). TRAF-2

signaling subsequently leads to the activation of NF�B which in-

duces the expression of anti-apoptotic genes such as FLIP and

Bcl-2.

Fo r m o r e i n f o r m a t i o n o n p r o d u c t s r e l a t e d t o A p o p t o s i s

Ad a p t o r Pr o t e i n s, p l e a s e v i s i t o u r w e b s i t e a t

www.RnDSystems.com/go/ApoptosisAdaptor

Detection of FADD in Human Cell Lysates. FADD was detected in lysates prepared

from human A431 epithelial carcinoma cells, Ramos and Raji Burkitt’s lymphoma-

derived cells, and MCF-7 breast cancer cells by Western blot analysis using anti-human

FADD polyclonal antibody (Catalog # AF2938).

Intracellular Detection of TRADD in Human Lymphocytes by Flow Cytometry.

Human lymphocytes were stained with APC-conjugated anti-human TRADD

monoclonal antibody (Catalog # IC2658A; filled histogram) or with APC-conjugated

mouse IgG2A isotype control antibody (Catalog # IC003A; open histogram).

DAXXANTIBODIES


DAXXaa 497-626


FADDANTIBODIES


FADD Human None Polyclonal Goat IgG WB AF2938 100 µg

RIP1ANTIBODIES


RIP1 Human/Mouse/Rat None 334640 Mouse IgG1 WB MAB3585 100 µg

TRADDANTIBODIES


TRADD Human None Polyclonal Goat IgG IHC, WB AF2658 100 µg

TRADD Human APC 313203 Mouse IgG2A FC IC2658A 100 Tests

TRADD Human Fluorescein 313203 Mouse IgG2A FC IC2658F 100 Tests

TRADD Human PE 313203 Mouse IgG2A FC IC2658P 100 Tests

TRADD Human None 313203 Mouse IgG2A FC, WB MAB2658 100 µg

Anti-Apoptotic Signaling Adaptors

14-3-3ANTIBODIES


14-3-3 Human/Mouse/Rat/Xenopus

None Polyclonal Rabbit IgG WB PPS043 100 µL

Phospho-14-3-3 (S58) Human/Mouse/Rat/Xenopus

None Polyclonal Rabbit IgG WB PPS042 100 µL

14-3-3�ANTIBODIES


14-3-3� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4724 100 µg

14-3-3�ANTIBODIES



14-3-3�ANTIBODIES







14-3-3�ANTIBODIES



14-3-3�ANTIBODIES



14-3-3�RECOMBINANT & NATURAL PROTEINS


14-3-3�HRP-conjugated

Human Solution with a carrier protein HRP E. coli HRP2669 250 µL

ANTIBODIES


14-3-3� Human/Mouse None Polyclonal Goat IgG WB AF2669 100 µg

CD2APANTIBODIES


CD2APaa 423-580

Human/Mouse/Rat None Polyclonal Sheep IgG WB AF4474 100 µg

DFF45/ICADANTIBODIES


DFF45/ICAD Human None Polyclonal Goat IgG WB AF4679 100 µg

FLIPRECOMBINANT & NATURAL PROTEINS


FLIPWestern Blot Standard

Human Lyophilized without a carrier protein None E. coli WBC001 1 Vial

ANTIBODIES


FLIPaa 432-450


SODD/BAG4ANTIBODIES


SODD/BAG4aa 122-457


SODD/BAG4 Mouse/Rat None Polyclonal Goat IgG WB AF4349 100 µg




Other Apoptosis-related Signaling Molecules

MyD88ANTIBODIES


MyD88 Human None Polyclonal Goat IgG FC, IHC, WB AF2928 100 µg

MyD88 Human None 316603 Rat IgG2A IHC MAB2928 100 µg

MyD88 Human None 316628 Rat IgG2B FC MAB29281 100 µg

MyD88 Mouse None 316902 Rat IgG2A IHC MAB3109 100 µg

MyD88 Mouse/Rat None Polyclonal Goat IgG FC, IHC, WB AF3109 100 µg



MyD88 Human 312.5 - 20000 pg/mL DY2928 15 Plates


TANKANTIBODIES


TANKaa 157-448

Human/Mouse None Polyclonal Goat IgG WB AF4755 100 µg

TRAF-1ANTIBODIES


TRAF-1 Human None Polyclonal Goat IgG WB AF3276 100 µg

TRAF-2ANTIBODIES



TRAF-2 Human/Mouse/Rat None 324522 Mouse IgG2A WB MAB3277 100 µg

TRAF-3ANTIBODIES


TRAF-3Isoform 2


TRAF-3Isoform 2

Human/Mouse/Rat None 433113 Rat IgG2A WB MAB3278 250 µg





TRAF-4ANTIBODIES



TRAF-4 Human None 433709 Rat IgG2A WB MAB3279 250 µg

TRAF-6ANTIBODIES



TRAM/TICAM2ANTIBODIES


TRAM/TICAM2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF4348 100 µg




Intrinsic Pathway of Caspase Activation



The intrinsic pathway of caspase activation can be initiated in re-

sponse to DNA damage, growth factor withdrawal, loss of contact

with the extracellular matrix, or glucocorticoids. These conditions

promote signaling pathways that lead to a loss in the integrity of

the mitochondrial membrane and caspase activation. The Bcl-2

family comprises a group of structurally related proteins that play a

fundamental role in the regulation of the intrinsic pathway by con-

trolling mitochondrial membrane permeability and the release of

the pro-apoptotic factor, Cytochrome c. Bcl-2 proteins are grouped

into three classes: those that inhibit apoptosis (Bcl-2, Bcl-xL, Bcl-w,

Mcl-1, Bcl-10, and Bcl-2 related protein A1); those that promote

apoptosis (BAK, Bax, Bcl-rambo, Bcl-xs, BOK/Mtd); and the

pro-apoptotic BH3-only proteins that bind and regulate the

anti-apoptotic Bcl-2 proteins (Bad, BID, Bik/Nbk, BIM, BLK, Bmf,

Hrk/DP5). Bag-1, BNIP3, BNIP3L are Bcl-2-interacting proteins that

are also involved in modulating apoptosis. The balance between

pro- and anti-apoptotic family members determines whether or

not a cell will undergo apoptosis. Bcl-2 and Bcl-xL

inhibit apoptosis

by binding to the pro-apoptotic Bax and BAK proteins. In healthy

cells, BAD is phosphorylated and sequestered in the cytoplasm by

the adapter protein, 14-3-3. When cytoplasmic levels of free Bad in-

crease, Bcl-2 and Bcl-xL

bind to Bad and release Bax and BAK. Bax

and BAK, or processed forms of these proteins, can then insert into

the mitochondrial membrane and cause the release of

Cytochrome c. Cleavage of BID as a result of Caspase-8, or

Caspase-10 activation following death receptor ligation also pro-

motes Bax/BAK-mediated release of Cytochrome c.

For more information on products related to the Bcl-2 Family, please

visit our website at www.RnDSystems.com/go/Bcl2

Alignment of the BH Domains in the Bcl-2 Family. (A) Position of the Bcl-2 homology regions (BH1-4) and �-helices in Bcl-2 and Bcl-xL. The regions forming the hydrophobic

pocket, the BH3-ligand, and pore are indicated. (B) Alignment of the BH regions in the Bcl-2 family. TM indicates a membrane targeting sequence.

Bcl-2 Family



Bcl-xL Inhibition of tBID-induced Cytochrome c Release. Isolated mouse liver mito-

chondria were incubated with Caspase-8-cleaved recombinant human BID (Catalog #

882-B8; purple line) or with cleaved recombinant mouse BID (Catalog # 883-M8; blue

line) and increasing concentrations of Bcl-xL (Catalog # 878-BC). The amount of

Cytochrome c released was measured using the rat/mouse Cytochrome c ELISA Kit

(Catalog # MCTC0). The blank sample (filled diamond) indicates spontaneously re-

leased Cytochrome c; the total sample (X) indicates the total amount of Cytochrome c

in the mitochondria at the start of the experiment. Results are the averages of triplicate

measurements +/- SEM.

Quantification of Bcl-2 in Cell Lysates. The levels of Bcl-2 were assessed in lysates

prepared from human KG1 myelogenous leukemia cells and human MCF-7 breast can-

cer cells using the human Bcl-2 DuoSet® IC ELISA Development System (Catalog #

DYC827B; histogram). The same lysates were also analyzed by Western blot using

anti-human Bcl-2 monoclonal antibody (Catalog # MAB827; inset).

Quantification of Bcl-xL in Cell Lysates. The levels of Bcl-xL were assessed in lysates

prepared from mouse NIH/3T3 fibroblast cells and human A431 carcinoma cells using

the human/mouse Bcl-xL DuoSet® IC ELISA Development System (Catalog # DYC894;

histogram). The same lysates were also analyzed by Western blot using anti-hu-

man/mouse Bcl-xL monoclonal antibody (Catalog # MAB827; inset).

Neutralization of tBID-induced Cytochrome c Release. Caspase-8-cleaved recombi-

nant human BID (Catalog # 882-B8; hBID), or cleaved recombinant mouse BID (Catalog

# 883-M8; mBID) was incubated alone or with anti-human/mouse BID polyclonal anti-

body (Catalog # AF860) for 30 (gray bars) or 60 minutes (yellow bars) prior to the

addition of isolated mouse liver mitochondria. The amount of Cytochrome c released

was measured using the rat/mouse Cytochrome c ELISA Kit (Catalog # MCTC0). The

blank sample indicates spontaneously released Cytochrome c; the total sample indi-

cates the total amount of Cytochrome c in the mitochondria at the start of the

experiment. Results are the averages of triplicate measurements.


BadANTIBODIES


Badaa 1- 21


BadN-Terminus


PRIMER PAIRS


Bad Human/Mouse/Rat RDP-41-025 25 Tests

Bag-1ANTIBODIES


Bag-1 Human None GP3.10G3E2 Mouse IgG1 IP, WB MAB852 100 µg

Bag-1 Mouse None Polyclonal Goat IgG IP, WB AF815 100 µg

PRIMER PAIRS


Bag-1 Human/Mouse RDP-81-025 25 Tests

BAKRECOMBINANT & NATURAL PROTEINS


BAKBH3 Domainaa 72-87

Human Lyophilized without a carrier protein None Synthetic 881-BA-100 100 µg

BAKL78A BH3 Domainaa 72-87

Human Lyophilized without a carrier protein None Synthetic 879-BK-100 100 µg

ANTIBODIES


BAKaa 53-72


PRIMER PAIRS


BAK Human/Mouse RDP-42-025 25 Tests

BaxANTIBODIES


Baxaa 3-16

Human None YTH-2D2 Mouse IgG1 IP, WB 2282-MC-100 100 µg

BaxMinus C-Terminus

Human None Polyclonal Total Goat IgG IHC, WB AB820 400 µg

BaxMinus C-Terminus


Baxaa 12-33 (human)

Human/Mouse None Polyclonal Rabbit IgG IHC, WB AF820 30 µg

BaxMinus C-Terminus

Human/Mouse None 127602 Mouse IgG2A WB MAB883 100 µg

Baxaa 12-24 (human)

Human/Mouse/Rat None YTH-6A7 Mouse IgG1 IP, WB 2281-MC-100 100 µg

Baxaa 3-16

Mouse None YTH-5B7 Mouse IgG1 IP, WB 2280-MC-100 100 µg



Bcl-2 Family

Bax continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS


BaxTotal Protein



PRIMER PAIRS


Bax Human/Mouse/Rat RDP-43-025 25 Tests

Bcl-10ANTIBODIES


Bcl-10 Human None Polyclonal Goat IgG WB, IHC AF2889 100 µg

Bcl-10 Human/Mouse/Rat None 384202 Mouse IgG1 WB, IHC MAB2889 100 µg

Bcl-2RECOMBINANT & NATURAL PROTEINS


Bcl-2Minus C-Terminus

Human Solution without a carrier protein None E. coli 827-BC-050 50 µg

ANTIBODIES


Bcl-2aa 41-54

Human None YTH-8C8 Mouse IgG1 IP, WB 2291-MC-100 100 µg





Bcl-2aa 1-208 (mouse)

Human/Mouse None Polyclonal Goat IgG IHC, IP, WB AF810 100 µg

Bcl-2aa 4-22



Mouse None 121529 Rat IgG2B WB MAB810 100 µg

Bcl-2aa 61-76 (mouse)

Mouse/Rat None YTH-10C4 Mouse IgG1 IP, WB 2290-MC-100 100 µg



Bcl-2Total Protein

Human 62.5 - 4000 pg/mL DYC827B-2 2 Plates


PRIMER PAIRS


Bcl-2 Human/Mouse/Rat RDP-44-025 25 Tests





Bcl-2 related protein A1RECOMBINANT & NATURAL PROTEINS


Bcl-2 related protein A1Minus C-terminusaa 1-152

Human Solution without a carrier protein None E. coli 1160-A1-050 50 µg

Bcl-2 related protein A1Western Blot Standard

Mouse Solution without a carrier protein None N/A WBC011 1 Vial

ANTIBODIES


Bcl-2 related protein A1aa 1-152


Bcl-wRECOMBINANT & NATURAL PROTEINS


Bcl-wMinus C-Terminus

Human Solution without a carrier protein None E. coli 824-BW-050 50 µg

Bcl-wWestern Blot Standard


ANTIBODIES


Bcl-waa 1-172


Bcl-waa 1-172


Bcl-waa 1-172

Human/Mouse None Polyclonal Goat IgG IHC AF8241 100 µg

Bcl-waa 32-51


PRIMER PAIRS


Bcl-w Human/Mouse RDP-125-025 25 Tests

Bcl-xANTIBODIES


Bcl-xaa 49-68

Human None Polyclonal Sheep IgG IP, WB AF800 50 µg

Bcl-xL

RECOMBINANT & NATURAL PROTEINS


Bcl-xL

Minus C-TerminusHuman Solution without a carrier protein None E. coli 894-BX-050 50 µg

Bcl-xL

Minus C-TerminusMouse Solution without a carrier protein None E. coli 878-BC-050 50 µg

ANTIBODIES


Bcl-xL

aa 3-14 (human)Human/Mouse/Rat None YTH2H12 Mouse IgG2A IP, WB 2300-MC-100 100 µg



Bcl-2 Family

Bcl-xL continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS


Bcl-xL

Total ProteinHuman 625 - 20000 pg/mL DYC894-2 2 Plates


BIDRECOMBINANT & NATURAL PROTEINS


BID Human Solution without a carrier protein None E. coli 846-BD-050 50 µg

BIDCaspase-8-cleaved

Human Solution without a carrier protein None E. coli 882-B8-050 50 µg

BID Mouse Solution without a carrier protein None E. coli 860-MB-050 50 µg

BIDCaspase-8-cleaved

Mouse Solution without a carrier protein None E. coli 883-M8-050 50 µg

ANTIBODIES


BIDaa 34-52


BID Human/Mouse None Polyclonal Goat IgG IP, WB AF860 100 µg

BID Mouse Biotin Polyclonal Goat IgG WB BAF860 50 µg

BID Mouse None 91508 Rat IgG1 WB MAB860 100 µg

PRIMER PAIRS


BID Human RDP-110-025 25 Tests

BID Mouse RDP-111-025 25 Tests

Bik-like Killer ProteinRECOMBINANT & NATURAL PROTEINS


Bik-like Killer ProteinWestern Blot Standard

Mouse Solution without a carrier protein None E. coli WBC007 1 Vial

ANTIBODIES


Bik-like Killer Proteinaa 25-42

Mouse None Polyclonal Rabbit IgG WB AF849 100 µg

BIMRECOMBINANT & NATURAL PROTEINS


BIMLong Form

Human Solution without a carrier protein None E. coli 1325-BL-050 50 µg

BNIP3ANTIBODIES


BNIP3 Human None Polyclonal Goat IgG WB AF4147 100 µg




BNIP3LANTIBODIES


BNIP3Laa 2-184

Human/Mouse/Rat None Polyclonal Goat IgG WB AF4030 100 µg

BOKRECOMBINANT & NATURAL PROTEINS


BOKWestern Blot Standard

Rat Lyophilized without a carrier protein None E. coli WBC004 1 Vial

ANTIBODIES


BOKaa 41-61

Rat None Polyclonal Rabbit IgG WB AF825 80 µg

HrkRECOMBINANT & NATURAL PROTEINS


HrkWestern Blot Standard


ANTIBODIES


Hrkaa 47-66


Mcl-1RECOMBINANT & NATURAL PROTEINS


Mcl-1Western Blot Standard


ANTIBODIES


Mcl-1aa 175-194


PRIMER PAIRS


Mcl-1 Human/Mouse RDP-82-025 25 Tests




Bcl-2 Family

Mitochondrial Proteins



An alteration in the permeability of the mitochondrial membrane is

a hallmark of the intrinsic pathway of caspase activation. This

change in permeability leads to the cytosolic release of multiple

proteins, including Cytochrome c, Smac/Diablo, HTRA2/Omi,

Apoptosis Inducing Factor (AIF), and Endonuclease G from the

intermembrane space of the mitochondria. The release of these

proteins, which are normally sequestered, has severe conse-

quences for the cell. For example, cytoplasmic Cytochrome c and

dATP initiate the assembly of the apoptosome with APAF-1 and

Pro-caspase-9. The assembly of this complex promotes the activa-

tion of Caspase-9 which subsequently leads to the activation of

Caspase-3, -6, and -7 to induce apoptosis. In addition, Smac/Diablo

and HTRA2/Omi interact with the IAP proteins disrupting their abil-

ities to inhibit caspase activity, while AIF and Endonuclease G

translocate to the nucleus upon mitochondrial release and pro-

mote chromatin condensation and DNA fragmentation.

For more information on Mitochondrial Protein products, please visit

our website at www.RnDSystems.com/go/Mitochondria

Pro-apoptotic Proteins Released from the Mitochondria. The intrinsic pathway of caspase activation or Caspase-8-dependent cleavage of BID causes a change in the perme-

ability of the mitochondrial membrane leading to the release of multiple pro-apoptotic proteins, including Cytochrome c, SMAC/Diablo, Apoptosis Inducing Factor (AIF) and

Endonuclease G (Endo G).


AIFANTIBODIES


AIF Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1457 100 µg

Cytochrome cRECOMBINANT & NATURAL PROTEINS


Cytochrome cNatural Source

Human Lyophilized without a carrier protein None Human Heart 709-CCH-010 10 µg

ANTIBODIES


Cytochrome c Human/Equine None 2B5F8 Mouse IgG2A IHC, IP MAB898 100 µg

Cytochrome c Human/Mouse/Rat None 4CytC-21 Mouse IgG1 WB 6370-MC-100 100 µg

Cytochrome c Human/Mouse/Rat None 7H8.2C12 Mouse IgG2B WB MAB897 100 µg

Cytochrome c Human/Mouse/Rat None 2.7D5 Mouse IgG2A IP MAB899 100 µg



Kinetics of tBID-induced Cytochrome c Release.

Isolated mouse liver mitochondria were untreated (orange

line), or incubated with either 52 nM (light blue line), or

5.2 nM (green line) Caspase-8-cleaved recombinant hu-

man BID (Catalog # 882-B8) for the indicated times. The

amount of Cytochrome c released was determined using

the rat/mouse Cytochrome c ELISA (Catalog # MCTC0).

Inhibition of XIAP Activity by SMAC/Diablo.

Cytochrome c and dATP-activated human Jurkat T cell

extracts were treated with recombinant human XIAP

(Catalog # 822-XF; 500 nM) and increasing concentra-

tions of recombinant human SMAC/Diablo (Catalog

#789-SM) prior to the addition of the caspase peptide

substrate, DEVD-afc. DEVD-afc cleavage was monitored

in a fluorescence plate reader.

SMAC/Diablo in Jurkat and CHP-100 Cells. Cell lysates

from human Jurkat T cells and CHP-100 neuroblastoma

cells were assayed for levels of total SMAC/Diablo using

the human SMAC/Diablo DuoSet® IC ELISA Development

System (Catalog # DYC789; histogram). The lysates were

also analyzed by Western blot using an anti-human

SMAC/Diablo polyclonal antibody (Catalog # AF789; inset).

Mitochondrial Proteins

Cytochrome c continuedELISAs/ASSAYS


Cytochrome c Human � Cell Lysate

� Subcellular Fractions(optimal protocol to bedetermined by end user)

0.31 ng/mL 0.625-20 ng/mL DCTC0 1 Kit

Cytochrome c Mouse/Rat � Cell Lysate

� Subcellular Fractions(optimal protocol to bedetermined by end user)

0.5 ng/mL 0.78-25 ng/mL MCTC0 1 Kit



Cytochrome cTotal Protein-Short Format

Mouse/Rat N/A DYC897-2 2 Plates


Holocytochrome cANTIBODIES


Holocytochrome c Human/Mouse/Rat None 2CtyC-199 Mouse IgG1 IP, WB 6380-MC-100 100 µg

HTRA2/OmiRECOMBINANT & NATURAL PROTEINS


HTRA2/Omi Human Solution without a carrier protein None E. coli 1458-HT-100 100 µg

ANTIBODIES


HTRA2/Omi Human None 229926 Mouse IgG2B IHC, WB MAB1458 100 µg

HTRA2/Omi Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1458 100 µg

SMAC/DiabloRECOMBINANT & NATURAL PROTEINS


SMAC/Diablo Human Solution without a carrier protein None E. coli 789-SM-100 100 µg

ANTIBODIES


SMAC/Diablo Human None Polyclonal Rabbit IgG WB AF789 100 µg

SMAC/Diablo Human None Polyclonal Goat IgG IHC, WB AF7891 100 µg

SMAC/Diablo Human Biotin Polyclonal Goat IgG WB BAF789 50 µg



SMAC/DiabloTotal Protein



UCP2ANTIBODIES


UCP2 Human/Mouse None Polyclonal Goat IgG WB AF4739 100 µg




Oxidative Stress



Oxidative stress is a condition characterized by elevated levels of

intracellular reactive oxygen species (ROS). ROS either are, or break

down to form, free radicals. ROS include superoxide anion (O2-),

hydrogen peroxide (H2O

2), and hydroxyl radicals (OH-) that are

capable of reacting with, and damaging DNA, proteins, and lipids.

Under normal conditions, ROS are cleared from the cell by the ac-

tions of superoxide dismutase, catalase, glutathione peroxidase,

thioredoxin, and other molecules. Low levels of intracellular ROS

have also been identified as second messengers in signaling path-

ways and implicated in transcriptional regulation to promote cell

proliferation, but higher doses of ROS result in growth arrest and

cell death. Bcl-2 inhibits free-radical induced apoptosis. Whether

the result is cell survival or apoptosis, oxidative stress activates nu-

merous intracellular signaling pathways such as Akt, NF�B, p53,

Jak/Stat, and MAPK to affect changes in gene expression.

For more information on products related to Oxidative Stress, please

visit our website at www.RnDSystems.com/go/OxStress

Oxidative Stress and the Mitochondria. Mitochondrial-derived ROS, such as H2O2, generated by defects in the electron transport chain (ETC Complex I) can react with iron or

copper in the cytoplasm to generate free radicals. ROS can also be generated by nitric oxide synthase (NOS) which is activated by faulty cytoplasmic calcium homeostasis and

mitochondrial calcium accumulation. In addition, mitochondrial calcium accumulation and a decrease in mitochondrial ROS scavenging capability leads to the opening of the

mitochondrial transition pore (MTP) and the release of Cytochrome c. Free radicals and cytoplasmic Cytochrome c promote apoptosis.

Detection of SOD1 in Human Cell Lysates. SOD1 was detected in cell lysates pre-

pared from human Jurkat T cells and Ramos Burkitt’s lymphoma cells by Western blot

analysis using anti-human SOD1 monoclonal antibody (Catalog # MAB3418; left). This

antibody failed to recognize recombinant human SOD2 or SOD3, demonstrating

specificity for SOD1 (right).

eNOS Expression in Human Placenta. eNOS was detected in paraffin-embedded

tissue sections of human placenta using anti-human eNOS polyclonal antibody

(Catalog # AF950). Tissues were stained using anti-goat HRP-AEC Cell and Tissue Stain-

ing Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).

Oxidative Stress

Carboxymethyl LysineANTIBODIES


Carboxymethyl Lysine Multi-species None 318003 Mouse IgG2B WB MAB3247 100 µg

CatalaseANTIBODIES


Catalase Human/Mouse/Rat None Polyclonal Goat IgG WB AF3398 100 µg

Glutaredoxin 1ANTIBODIES


Glutaredoxin 1 Human/Mouse None Polyclonal Goat IgG WB AF3399 100 µg

Glutaredoxin 1 Mouse None Polyclonal Goat IgG WB AF3119 100 µg

Glutathione Peroxidase 1ANTIBODIES


Glutathione Peroxidase 1 Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF3798 100 µg

Glutathione Peroxidase 3ANTIBODIES


Glutathione Peroxidase 3aa 74-226

Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF4199 100 µg

Glyoxalase IRECOMBINANT & NATURAL PROTEINS


Glyoxalase I Human Lyophilized without a carrier protein None E. coli 4959-GL-02M 2500 µg

HO-1/HMOX1/HSP32ANTIBODIES


HO-1/HMOX1/HSP32aa 1-261 (human)



Human/Mouse None 412811 Rat IgG2B WB MAB3776 100 µg

HO-1/HMOX1/HSP32 Mouse/Rat None Polyclonal Goat IgG WB AF3169 100 µg



HO-1/HMOX1/HSP32Total Protein

Human N/A DYC3776-2 2 Plates


HO-2/HMOX2ANTIBODIES


HO-2/HMOX2 Human/Mouse None 322913 Mouse IgG2A WB MAB3170 100 µg

HO-2/HMOX2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3170 100 µg




4-HydroxynonenalANTIBODIES


4-Hydroxynonenal Multi-species None 198960 Mouse IgG2B IHC, WB MAB3249 100 µg

MyeloperoxidaseRECOMBINANT & NATURAL PROTEINS


Myeloperoxidase Human Lyophilized without a carrier protein None NS0 3174-MP-250 250 µg

ANTIBODIES


Myeloperoxidase Human None Polyclonal Goat IgG IHC, WB AF3174 100 µg

Myeloperoxidase Human Biotin Polyclonal Goat IgG WB BAF3174 50 µg

Myeloperoxidase Human/Mouse None Polyclonal Goat IgG IHC, WB AF3667 100 µg

Myeloperoxidase Human/Mouse None 392105 Mouse IgG2B IHC, WB MAB3174 100 µg

ELISAs/ASSAYS


Myeloperoxidase Human � Serum



� Urine

� Saliva

� Cell Lysate

� Breast Milk

0.618 ng/mL 1.56-100 ng/mL DMYE00 1 Kit

Nitric OxideELISAs/ASSAYS


Nitric Oxide Multi-species � Serum



� Urine

0.78 nmol/mL 3.12-200 nmol/mL KGE001 1 Kit

NitrotyrosineANTIBODIES


Nitrotyrosine Multi-species None 306507 Mouse IgG3 WB MAB3248 100 µg

eNOSANTIBODIES


eNOSaa 1030-1203

Human None Polyclonal Goat IgG IHC, IP, WB AF950 100 µg

eNOSaa 1030-1203

Human Biotin Polyclonal Goat IgG IHC, WB BAF950 50 µg

ELISAs/ASSAYS


eNOS Human � Cell Lysate 25 pg/mL 62.5-4000 pg/mL DEN00 1 Kit




Oxidative Stress

eNOS continuedPRIMER PAIRS


eNOS Human/Mouse RDP-94-025 25 Tests

iNOSANTIBODIES


iNOSaa 781-798

Human None 2D2-B2 Mouse IgG1 IHC, WB MAB9502 100 µg

ELISAs/ASSAYS


iNOS Human � Cell Lysate 0.46 U/mL 1.25-80 U/mL DNS00 1 Kit

PRIMER PAIRS


iNOS Human/Mouse RDP-101-025 25 Tests

nNOSANTIBODIES


nNOS Human None Polyclonal Goat IgG IHC, WB AF2416 100 µg

PRIMER PAIRS


nNOS Human/Mouse RDP-93-025 25 Tests

Park7/DJ-1ANTIBODIES


Park7/DJ-1 Human None Polyclonal Goat IgG IHC, WB AF3995 100 µg

Park7/DJ-1 Human Biotin Polyclonal Goat IgG WB BAF3995 50 µg

Park7/DJ-1 Human/Mouse None Polyclonal Goat IgG WB AF3668 100 µg

Peroxiredoxin 1ANTIBODIES


Peroxiredoxin 1 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3488 100 µg






Peroxiredoxin 5 Human/Mouse None Polyclonal Goat IgG WB AF3774 100 µg







PON2ANTIBODIES


PON2aa 30-354


PON3ANTIBODIES


PON3aa 30-354


SOD1/Cu-Zn SODANTIBODIES


SOD1/Cu-Zn SOD Human None 348808 Mouse IgG2A IHC, WB MAB3418 100 µg

SOD1/Cu-Zn SOD Human/Mouse None Polyclonal Goat IgG WB AF3418 50 µg

SOD1/Cu-Zn SOD Mouse/Rat None Polyclonal Goat IgG WB AF3787 100 µg

SOD2/Mn-SODANTIBODIES


SOD2/Mn-SODaa 25-222

Human/Mouse/Rat None 349810 Mouse IgG1 IHC, WB MAB3419 100 µg

SOD3/EC-SODANTIBODIES


SOD3/EC-SODaa 25-251

Mouse None Polyclonal Goat IgG WB AF4817 100 µg

Superoxide DismutaseELISAs/ASSAYS


Superoxide Dismutase Multi-species � Cell Extracts N/A N/A 7500-100-K 100 Tests

Thioredoxin-1RECOMBINANT & NATURAL PROTEINS


Thioredoxin-1 Human Lyophilized without a carrier protein None E. coli 1970-TX-500 500 µg

ANTIBODIES


Thioredoxin-1 Human None Polyclonal Goat IgG IHC, WB AF1970 100 µg

Thioredoxin-1 Human Biotin Polyclonal Goat IgG WB BAF1970 50 µg




Oxidative Stress

Thioredoxin-2ANTIBODIES


Thioredoxin-2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3254 100 µg

Thioredoxin-80RECOMBINANT & NATURAL PROTEINS


Thioredoxin-80 Human Lyophilized without a carrier protein None E. coli 2329-TX-100 100 µg

Thioredoxin-like 5/TRP14ANTIBODIES


Thioredoxin-like 5/TRP14 Human None Polyclonal Goat IgG WB AF3504 100 µg

Thioredoxin-like 5/TRP14 Mouse/Rat None Polyclonal Goat IgG WB AF3769 100 µg

TXNDC5ANTIBODIES


TXNDC5aa 110-432





Heat Shock Proteins (HSPs)



Heat shock proteins (HSPs) are a highly conserved family of stress

response proteins that are induced upon exposure to environmen-

tal stresses such as heat, cold, or oxidative stress. Most HSPs act as

molecular chaperones to facilitate the folding of cellular proteins,

prevent protein aggregation, and target improperly folded pro-

teins to specific degradative pathways. In addition, a subset of HSPs

regulate apoptotic signaling pathways. While some HSPs are

anti-apoptotic (HSP27, HSP70, HSP90), others promote apoptosis

(HSP10, HSP60). HSP27, HSP70, and HSP90 all inhibit the forma-

tion of the apoptosome by binding to either Cytochrome c

(HSP27), or APAF-1 (HSP70, HSP90), subsequently preventing the

maturation and activation of Caspase-9. HSP70 can also inhibit

apoptosis by blocking the release of Apoptosis Inducing Factor

(AIF) from the mitochondria. HSP60, with or without HSP10, pro-

motes the proteolytic processing of Pro-caspase-3 to induce

apoptosis.

For more information on HSP-related products, please visit our website

at www.RnDSystems.com/go/HSPs

Regulation of Apoptotic Signaling by the Heat Shock Proteins. Some heat shock proteins inhibit apoptosis by binding to Cytochrome c or APAF-1 to prevent the formation

of the apoptosome. Other heat shock proteins promote apoptosis by stimulating the activation of Caspase-3.

HSP27 Inhibits Caspase Activity in Activated Cell Ex-

tracts. Human Jurkat T cell extracts were incubated with

Cytochrome c, dATP, and increasing concentrations of re-

combinant human HSP27 (Catalog # 1580-HS) prior to

the addition of the DEVD-afc caspase peptide substrate.

DEVD-afc cleavage was monitored in a fluorescence

plate reader.

Detection of HSP70 in Human Jurkat and Mouse

NIH/3T3 Cells by Western Blot. Human Jurkat T cells

and mouse NIH/3T3 fibroblast cells were either un-

treated (-), or heat shocked (+) for 30 minutes at 42° C

and then allowed to recover for 3 hours. HSP70 in lysates

from treated and non-treated cells was detected by

Western blot analysis using anti-human/mouse/rat

HSP70 monoclonal antibody (Catalog # MAB1663).

Quantification of Phosphor ylated HSP27 in

UV-treated mouse C2C12 Cells by ELISA. Mouse

C2C12 myoblast cells were left untreated or were

treated with UV (200 J/m2). Cell lysates were assessed for

HSP27 phosphorylation on serines 78 and 82 using the

human/mouse/rat Phospho-HSP27 (S78/82) DuoSet® IC

ELISA Development System (Catalog # DYC2314; histo-

gram). The same lysates were also analyzed by Western

blot ( inset ) using anti -human/mouse/rat

Phospho-HSP27 (S78/S82) polyclonal antibody (Catalog

# AF2314) and anti-human/mouse/rat HSP27 polyclonal

antibody (Catalog # AF15801).

Heat Shock Proteins

Clusterin/ApolipoproteinANTIBODIES


Clusterin Human None Polyclonal Goat IgG IHC, IP, WB AF2937 100 µg

Clusterin Human None 350227 Mouse IgG2B IP, WB MAB2937 100 µg

Clusterin Human None 350217 Mouse IgG2B IHC MAB29371 100 µg

Clusterin Mouse None Polyclonal Goat IgG IHC, IP, WB AF2747 100 µg

Clusterin Mouse Biotin Polyclonal Goat IgG WB BAF2747 50 µg

Clusterin� Chain

Mouse None 327020 Rat IgG2A IP, WB MAB2747 100 µg

�A Crystallin/CRYAAANTIBODIES


�A Crystallin/CRYAA Human/Mouse None Polyclonal Goat IgG WB AF4848 100 µg

�B Crystallin/CRYABANTIBODIES


�B Crystallin/CRYAB Human/Mouse/Rat None Polyclonal Goat IgG WB AF4849 100 µg

GRP75/HSPA9BANTIBODIES


GRP75/HSPA9Baa 542-679


GRP75/HSPA9Baa 645-649

Human/Mouse/Rat None 419612 Mouse IgG3 WB MAB3584 100 µg

GRP78/HSPA5ANTIBODIES


GRP78/HSPA5 Human/Mouse/Rat None Polyclonal Goat IgG WB AF4846 100 µg

HO-1/HMOX1/HSP32ANTIBODIES





Human/Mouse None 412811 Rat IgG2B WB MAB3776 100 µg

HO-1/HMOX1/HSP32 Mouse/Rat None Polyclonal Goat IgG WB AF3169 100 µg



HO-1/HMOX1/HSP32Total Protein






HSP10/EPFANTIBODIES


HSP10/EPF Human None 330629 Mouse IgG2B WB MAB3298 100 µg

HSP10/EPFaa 2-102


HSP10/EPF Mouse Biotin Polyclonal Goat IgG WB BAF2584 50 µg

HSP10/EPFaa 2-102

Mouse None Polyclonal Goat IgG WB AF2584 100 µg

HSP20/HSPB6ANTIBODIES


HSP20/HSPB6 Rat None Polyclonal Goat IgG WB AF4200 100 µg

HSP27RECOMBINANT & NATURAL PROTEINS


HSP27 Human Solution without a carrier protein None E. coli 1580-HS-050 50 µg

ANTIBODIES


HSP27 Human/Mouse None Polyclonal Rabbit IgG WB AF1580 50 µg

HSP27 Human/Mouse/Rat None Polyclonal Goat IgG WB AF15801 50 µg

Phospho-HSP27 (S78/S82) Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF2314 50 µg



HSP27Total Protein


Phospho-HSP27 (S78/S82) Human/Mouse/Rat 62.5 - 4000 pg/mL DYC2314-2 2 Plates


HSP40/DNAJB1ANTIBODIES


HSP40/DNAJB1 Human/Mouse None Polyclonal Sheep IgG WB AF4145 100 µg

HSP40/DNAJB1 Human/Mouse/Rat None 419401 Mouse IgG2B WB MAB4145 100 µg

HSP60ANTIBODIES


HSP60 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1800 50 µg

HSP60 Human/Mouse/Rat None 264233 Mouse IgG2B IHC, WB MAB1800 100 µg



HSP60Total Protein






Heat Shock Proteins

HSP70ANTIBODIES


HSP70 Human/Mouse None 242707 Mouse IgG2A IHC, WB MAB1663 100 µg

HSP70 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1663 50 µg

ELISAs/ASSAYS


HSP70Total ProteinSurveyor IC ELISA

Human/Mouse/Rat � Cell Lysate N/A 156-10000 pg/mL SUV1663 1 Kit



HSP70Total Protein

Human/Mouse/Rat 156 - 10000 pg/mL DYC1663-2 2 Plates


CELL-BASED ASSAYS

ANALYTE SPECIES CATALOG # SIZE

HSP70Cell-Based ELISA

Human/Mouse KCB1663 1 Kit

HSP90ANTIBODIES


HSP90 Human None Polyclonal Goat IgG WB AF3286 100 µg

HSP90 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3775 100 µg

HSP90 Human/Mouse/Rat None 341320 Mouse IgG2B WB MAB3286 100 µg

HSP90Isoform HSP90�

Human/Mouse/Rat None 341346 Mouse IgG2A WB MAB32861 100 µg

HSPA8/HSC71ANTIBODIES


HSPA8/HSC71aa 534-615


HSPH1ANTIBODIES


HSPH1aa 595-733







Intracellular signaling pathways regulate the decision of whether a

cell lives or dies. While death ligands such as Fas and TNF-� bind to

their receptors to promote caspase activation and cell death,

anti-apoptotic ligands, such as growth factors, bind to cell surface

receptors and activate intracellular kinases that promote cell sur-

vival. These kinases include PI 3-K, Akt/PKB, PKA, PKC, MEKs,

ERK1/2, p90RSK, and p38 MAP kinases. Intracellular kinases ulti-

mately activate or inhibit other cellular proteins involved in

apoptosis, or transcription factors that inhibit the expression of

pro-apoptotic proteins, or promote the expression of cell survival

proteins. For example, activation of PI 3-K leads to the

phosphorylation and activation of Akt/PKB resulting in the Akt-de-

pendent phosphorylation and inhibition of pro-apoptotic

molecules such as Bad, GSK-3, Caspase-9, and the Forkhead family

of transcription factors (FKHR). Similarly, other intracellular kinases

activate transcription factors such as CREB and STAT family mem-

bers which induce the expression of Bcl-2 and Bcl-xL, two Bcl-2

family members that protect the cell from apoptosis.

For more information on Intracellular Kinase products, please visit our

website at www.RnDSystems.com/go/ApoptosisICKinases

Intracellular Kinases Activated by Survival Signals Suppress Apoptosis. The binding of growth factors or cytokines to their receptors activates intracellular kinases that

phosphorylate and inhibit pro-apoptotic members of the Bcl-2 family, GSK-3, Caspase-9, and the Forkhead family of transcription factors. Intracellular kinases can also activate

transcription factors which induce the expression of Bcl-2 and Bcl-xL to promote cell survival (not shown).

Intracellular Detection of Phosphorylated Akt (S473)

in Jurkat Cells by Flow Cytometry. Human Jurkat T

cells, untreated (green histogram) or treated with the PI

3-kinase inhibitors LY294002 and wortmannin (purple

histogram), were stained using carboxyfluorescein

(CFS)-conjugated anti-human/mouse/rat Phospho-Akt

(S473) polyclonal antibody (Catalog # IC887F). Treated

cells were also stained with CFS-conjugated rabbit IgG

isotype control antibody (Catalog # IC105F; open histo-

gram).

Phosphorylated ERK1/ERK2 in Rat Brain Cortex.

Phosphorylation of ERK1/ERK2 was detected in cryostat

tissue sections of rat brain cortex using anti-hu-

man/mouse/rat Phospho-ERK1 (T202/Y204)/ERK2

(T185/Y187) polyclonal antibody (Catalog # AF1018). Tis-

sues were stained using anti-rabbit HRP-DAB Cell and

Tissue Staining Kit (Catalog # CTS005; brown) and

counterstained with hematoxylin (blue).

Phosphorylated MEK1/2 in PMA-treated Human

HeLa Cells. Human HeLa cells were uninduced or

treated with PMA (200 nM) for 20 minutes. Cell lysates

were assessed for MEK1 (S218/S222) and MEK2

(S222/S226) phosphorylation using the human

Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) DuoSet®

IC ELISA Development System (Catalog # DYC2506; his-

togram). The same lysates were also immunoblotted

(inset) using anti-human/mouse/rat Phospho-MEK1

(S218/S222)/MEK2 (S222/S226) polyclonal antibody

(Catalog # AF2506) and anti-human/mouse/rat

MEK1/MEK2 monoclonal antibody (Catalog # MAB2678).

Regulation of Pro- & Anti-Apoptotic Signaling PathwaysIntracellular Kinases

AktANTIBODIES


AktPan Specificaa 75-90


AktPan Specific

Human/Mouse/Rat APC 281046 Mouse IgG2B FC IC2055A 100 Tests

AktPan Specific

Human/Mouse/Rat Fluorescein 281046 Mouse IgG2B FC IC2055F 100 Tests

AktPan Specific

Human/Mouse/Rat PE 281046 Mouse IgG2B FC IC2055P 100 Tests

AktPan Specific

Human/Mouse/Rat None 281046 Mouse IgG2B FC, IHC, WB MAB2055 100 µg

Phospho-Akt (S473) Human/Mouse/Rat None Polyclonal Rabbit IgG FC, IHC, WB AF887 100 µg

Phospho-Akt (S473) Human/Mouse/Rat Fluorescein Polyclonal Rabbit IgG FC IC887F 100 Tests

Phospho-Akt (S473) Human/Mouse/Rat PE Polyclonal Rabbit IgG FC IC887P 100 Tests

ELISAs/ASSAYS


Phospho-Akt (S473)Pan SpecificSurveyor IC ELISA

Human/Mouse/Rat � Cell Lysate N/A 0.31-20 pg/mL SUV887 1 Kit



Phospho-Akt (S473)Pan Specific



CELL-BASED ASSAYS


Phospho-Akt (S473)Pan SpecificCell-Based ELISA

Human/Mouse/Rat KCB887 1 Kit

Akt1RECOMBINANT & NATURAL PROTEINS


Akt1Active

Human Solution without a carrier protein None Sf 9 1775-KS-010 10 µg

ANTIBODIES


Akt1 Human APC 302407 Mouse IgG1 FC IC17751A 100 Tests

Akt1 Human Fluorescein 302407 Mouse IgG1 FC IC17751F 100 Tests

Akt1 Human PE 302407 Mouse IgG1 FC IC17751P 100 Tests

Akt1 Human None 302407 Mouse IgG1 FC, IHC MAB17751 100 µg

Akt1 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1775 50 µg

Akt1 Human/Mouse/Rat None 232790 Mouse IgG2A WB MAB1775 100 µg

Akt1aa 1-149

Human/Mouse/Rat None Polyclonal Sheep IgG WB AF17751 100 µg




Akt1 continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS


Phospho-Akt1 (S473) Human/Mouse 312 - 20000 pg/mL DYC2289B-2 2 Plates

Akt1Total Protein



Akt2ANTIBODIES


Akt2 Human APC 302501 Mouse IgG1 FC IC23152A 100 Tests

Akt2 Human PE 302501 Mouse IgG1 FC IC23152P 100 Tests

Akt2 Human None 302501 Mouse IgG1 FC, IHC MAB23152 100 µg

Akt2 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF2315 50 µg

Akt2 Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF23151 100 µg

Akt2 Human/Mouse/Rat None 302514 Mouse IgG3 WB MAB2315 100 µg

Akt3ANTIBODIES


Akt3 Human None 199864 Mouse IgG1 WB MAB1463 100 µg

Akt3 Human None 199822 Mouse IgG1 FC MAB14631 100 µg

ASK1RECOMBINANT & NATURAL PROTEINS


ASK1Activeaa 649-946


ANTIBODIES


ASK1aa 1011-1196

Human None Polyclonal Sheep IgG IHC, WB AF3575 100 µg

CaMKK�ANTIBODIES


CaMKK�aa 1-132

Human/Rat None Polyclonal Sheep IgG WB AF4536 100 µg

ERK1/ERK2ANTIBODIES


ERK1/ERK2 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1576 100 µg

ERK1/ERK2 Human/Mouse/Rat None 216703 Mouse IgG1 IHC, WB MAB1576 100 µg

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)



Human/Mouse/Rat None 269434 Rabbit IgG WB MAB1018 100 µg




Intracellular Kinases

ERK1/ERK2 continuedELISAs/ASSAYS


Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)Surveyor IC ELISA







CELL-BASED ASSAYS


Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)Cell-Based ELISA


ERK1RECOMBINANT & NATURAL PROTEINS


ERK1Active


ANTIBODIES


ERK1 Human None Polyclonal Goat IgG WB AF1879 100 µg

ERK1 Human Biotin Polyclonal Goat IgG WB BAF1879 50 µg

ERK1aa 1-28


ERK1aa 1-28

Human None 250603 Mouse IgG2A IHC, WB MAB1940 100 µg

ERK1 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1575 50 µg



ERK1Total Protein


Phospho-ERK1 (T202/Y204) Human/Mouse/Rat 125 - 8000 pg/mL DYC1825-2 2 Plates


ERK2RECOMBINANT & NATURAL PROTEINS


ERK2Active

Human Solution without a carrier protein None E. coli 1230-KS-010 10 µg

ANTIBODIES


ERK2 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1230 100 µg

ERK2 Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF12301 100 µg

ERK2 Human/Mouse/Rat None 191801 Mouse IgG2A IHC, WB MAB1230 100 µg

ELISAs/ASSAYS


Phospho-ERK2 (T185/Y187)Surveyor IC ELISA

Human/Mouse/Rat N/A 0.19-12 ng/mL SUV1483 1 Kit




ERK2 continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS


ERK2Total Protein

Human/Mouse/Rat 312.5 - 20000 pg/mL DYC1230B-2 2 Plates

Phospho-ERK2 (T185/Y187) Human/Mouse/Rat 187 - 12000 pg/mL DYC1483-2 2 Plates


G�LANTIBODIES


G�L Human/Mouse/Rat None Polyclonal Goat IgG WB AF4004 100 µg

GSK-3�ANTIBODIES


GSK-3�aa 90-483

Human None 288901 Mouse IgG2B IHC MAB2986 100 µg

Phospho-GSK-3� (S21) Human/Mouse/Rat None Polyclonal Rabbit IgG2B IHC, WB AF4125 100 µg



Phospho-GSK-3� (S21) Human 156.25 to 10,000 pg/mL DYC4125-2 2 Plates


GSK-3�/�ANTIBODIES


GSK-3�/� Human/Mouse/Rat None Polyclonal Rabbit IgG FC, WB AF2157 50 µg

Phospho-GSK-3�/� (S21/S9) Human/Mouse/Rat None Polyclonal Rabbit IgG FC, IHC, WB AF1590 100 µg



GSK-3�/�Total Protein


Phospho-GSK-3�/� (S21/S9) Human/Mouse/Rat 156 - 10000 pg/mL DYC2630-2 2 Plates


GSK-3�RECOMBINANT & NATURAL PROTEINS


GSK-3�Active


ANTIBODIES


GSK-3� Human Fluorescein 272536 Rat IgG2A FC IC2506F 100 Tests

GSK-3� Human PE 272536 Rat IgG2A FC IC2506P 100 Tests

GSK-3� Human None 272535 Rat IgG2B IHC MAB25061 100 µg

GSK-3� Human/Mouse/Rat None 272536 Rat IgG2A FC, WB MAB2506 100 µg



Phospho-GSK-3� (S9) Human/Mouse/Rat 312 - 20000 pg/mL DYC1590-2 2 Plates






ss

Jak1ANTIBODIES


Phospho-Jak1 (Y1022/Y1023) Human None Polyclonal Rabbit IgG WB AF4510 100 µg

Jak1aa 20-274 (human)

Human/Mouse/Rat None 413104 Rat IgG2B FC, IHC, WB MAB4260 100 µg

Jak1aa 14-55 (mouse)

Mouse/Rat None Polyclonal Goat IgG IHC, WB AF602 100 µg

Jak2ANTIBODIES


Jak2aa 2-63 (mouse)

Mouse/Rat None Polyclonal Goat IgG WB AF2988 100 µg

Jak3ANTIBODIES


Jak3aa 46-209


JNKANTIBODIES


JNKPan Specific


JNKPan Specific


Phospho-JNK (T183/Y185) Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1205 50 µg



JNKTotal ProteinPan Specific


Phospho-JNK (T183/Y185)Pan Specific



JNK1/JNK2ANTIBODIES


JNK1/JNK2 Human/Mouse/Rat None 252323 Mouse IgG2A IHC, WB MAB2076 100 µg

JNK1RECOMBINANT & NATURAL PROTEINS


JNK1Active

Mouse Solution without a carrier protein None Sf 9 1776-KS-010 10 µg




JNK1 continuedANTIBODIES


JNK1 Human/Mouse/Rat None 228611 Mouse IgG1 WB MAB1776 100 µg

JNK1 Human/Mouse/Rat None 228601 Mouse IgG1 IHC, WB MAB17761 100 µg



JNK1Total Protein



JNK2ANTIBODIES


JNK2 Human/Mouse/Rat None 252320 Mouse IgG2B IHC, WB MAB1846 100 µg

JNK2aa 267-287

Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1846 100 µg



JNK2Total Protein


Phospho-JNK2 (T183/Y185) Human/Mouse/Rat 125 - 8000 pg/mL DYC2236-2 2 Plates


LMTK2ANTIBODIES


LMTK2 Human None 465222 Mouse IgG2B WB MAB5147 100 µg

MEK1ANTIBODIES


MEK1 Human/Mouse/Rat None Polyclonal Goat IgG WB AF2079 100 µg

MEK1 Human/Mouse/Rat None 239421 Mouse IgG2A WB MAB2079 100 µg

Phospho-MEK1 (T386) Human/Mouse/Rat/Bovine/Canine/Chicken/Primate/Xenopus

None Polyclonal Rabbit IgG WB PPS076 100 µg

Phospho-MEK1 (T292) Human/Mouse/Rat/Bovine/Canine/Primate

None Polyclonal Rabbit IgG WB PPS075 100 µg

MEK1/MEK2ANTIBODIES


MEK1/MEK2 Human/Mouse/Rat None 239416 Mouse IgG2B IHC, WB MAB2678 100 µg

Phospho-MEK1 (S218/S222)/MEK2 (S222/S226)




Phospho-MEK1 (S218/S222)/MEK2 (S222/S226)







ss

MEK1/MEK2 continuedCELL-BASED ASSAYS


Phospho-MEK1 (S218/S222)/MEK2 (S222/S226)Cell-Based ELISA


MEK2ANTIBODIES


MEK2 Human None Polyclonal Goat IgG WB AF2855 100 µg

MEK2 Human Biotin Polyclonal Goat IgG WB BAF2855 50 µg

MEK2 Human/Mouse/Rat None 300317 Mouse IgG2A IHC, WB MAB2855 100 µg

MKK3ANTIBODIES


MKK3 Human/Mouse/Rat None 275909 Mouse IgG2B IHC, WB MAB2515 100 µg

MKK3/MKK6ANTIBODIES


Phospho-MKK3 (S218/T222)/MKK6 (S207/T211)


MKK3/MKK6 Human/Mouse/Rat None 275922 Mouse IgG1 WB MAB2514 100 µg

MKK4ANTIBODIES


MKK4 Human None 323042 Mouse IgG2B IHC MAB3390 100 µg

Phospho-MKK4 (S257/T261) Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF2990 100 µg

MKK6RECOMBINANT & NATURAL PROTEINS


MKK6Active

Human Lyophilized without a carrier protein None E. coli 1604-MK-010 10 µg

ANTIBODIES


MKK6 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1604 50 µg

MKK6 Human/Mouse/Rat None Polyclonal Goat IgG WB AF16041 100 µg

MKK6 Human/Mouse/Rat Biotin Polyclonal Goat IgG WB BAF16041 50 µg

MKK6 Human/Mouse/Rat None 304328 Rat IgG2B IHC, WB MAB1604 100 µg

MKK7ANTIBODIES


MKK7 Human None Polyclonal Goat IgG IHC, WB AF3579 100 µg

MKK7 Human None 324302 Mouse IgG2B IHC MAB3579 100 µg




MLK3ANTIBODIES


MLK3aa 85-207


MLK4�ANTIBODIES


MLK4�aa 560-570


MSK1ANTIBODIES


MSK1 Human None 252608 Mouse IgG2B IHC MAB2518 100 µg

Phospho-MSK1 (S212) Human None Polyclonal Rabbit IgG IHC, WB AF1036 100 µg

MSK1 Human/Mouse None Polyclonal Goat IgG IHC, WB AF2518 100 µg

MSK1/MSK2ANTIBODIES


Phospho-MSK1 (S376)/MSK2 (S360)


MSK2ANTIBODIES


MSK2 Human/Mouse None 261034 Rat IgG2A WB MAB2310 100 µg

Phospho-MSK2 (S196) Human/Mouse None Polyclonal Rabbit IgG IHC, WB AF189 100 µg

MST1/STK4ANTIBODIES


MST1/STK4 Human None Polyclonal Goat IgG WB AF4939 100 µg

MST2/STK3ANTIBODIES


MST2/STK3aa 339-456


p38ANTIBODIES


Phospho-p38 (T180/Y182) Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF869 100 µg





p38 continuedCELL-BASED ASSAYS


Phospho-p38 (T180/Y182)Cell-Based ELISA


p38�RECOMBINANT & NATURAL PROTEINS


p38�Active

Human Solution without a carrier protein None E. coli 1806-P3-010 10 µg

p38�Unactive

Human Solution without a carrier protein None E. coli 869-P3-050 50 µg

ANTIBODIES


p38� Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF8691 100 µg

p38� Human/Mouse/Rat None 142102 Mouse IgG2B IHC, WB MAB869 100 µg

ELISAs/ASSAYS


Phospho-p38� (T180/Y182)Surveyor IC ELISA




p38�Total Protein

Human/Mouse/Rat 312 - 20000 pg/mL DYC8691B-2 2 Plates

Phospho-p38� (T180/Y182) Human/Mouse/Rat 62.5 - 4000 pg/mL DYC869-2 2 Plates


p38�ANTIBODIES


p38� Human None 280071 Mouse IgG2B IHC MAB3274 100 µg

p38ANTIBODIES


p38 Human None Polyclonal Rabbit IgG IHC, WB AF1519 100 µg

p38 Human None 197202 Mouse IgG1 WB MAB1519 500 µg



p38Total Protein


Phospho-p38 (T180/Y182) Human 156 - 10000 pg/mL DYC2124-2 2 Plates





p38ANTIBODIES


p38 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1347 100 µg

p38 Human/Mouse/Rat None Polyclonal Goat IgG WB AF1644 100 µg

p38 Human/Mouse/Rat None 212464 Mouse IgG1 WB MAB1347 100 µg



Phospho-p38 (T183/Y185) Human/Mouse 125 - 8000 pg/mL DYC1664-2 2 Plates

p38Total Protein



p70 S6 KinaseRECOMBINANT & NATURAL PROTEINS


p70 S6 KinaseActive


ANTIBODIES


Phospho-p70 S6 Kinase (T229) Human None Polyclonal Goat IgG IHC, WB AF896 100 µg

Phospho-p70 S6 Kinase (T229) Human None Polyclonal Rabbit IgG WB AF8961 100 µg

Phospho-p70 S6 Kinase (T389) Human None Polyclonal Rabbit IgG WB AF8963 100 µg

p70 S6 Kinase Human/Mouse/Rat None Polyclonal Rabbit IgG FC, IHC, WB AF8962 100 µg

p70 S6 Kinase Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF8964 100 µg

p70 S6 Kinase Human/Mouse/Rat None 215247 Mouse IgG1 FC, IHC, WB MAB8962 100 µg

Phospho-p70 S6 Kinase(T421/S424)




Phospho-p70 S6 Kinase (T389) Human 312 - 20000 pg/mL DYC896-2 2 Plates

p70 S6 KinaseTotal Protein

Human/Mouse/Rat 15.6 - 1000 pg/mL DYC8962-2 2 Plates

Phospho-p70 S6 Kinase(T421/S424)



CELL-BASED ASSAYS


Phospho-p70 S6 Kinase (T389)Cell-Based ELISA


p70 S6 Kinase�ANTIBODIES


p70 S6 Kinase� Human None 303421 Mouse IgG2B WB MAB2987 100 µg





PDK-1ANTIBODIES


PDK-1aa 296-314


PI 3-Kinase p85�ANTIBODIES


PI 3-Kinase p85�aa 328-431


PI 3-Kinase p110ANTIBODIES


PI 3-Kinase p110 Human/Mouse/Rat None 376315 Mouse IgG1 WB MAB4426 100 µg

PI 3-Kinase p110�ANTIBODIES


PI 3-Kinase p110�aa 153-349




PI 3-Kinase p110aa 391-542




PI 3-Kinase p110aa 298-467

Human None 304103 Rat IgG2A WB MAB2999 100 µg

PKA CANTIBODIES


PKA CPan Specific


PKA C�ANTIBODIES


PKA C� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4268 100 µg

PKA RI�ANTIBODIES


PKA RI� Human/Mouse/Rat None Polyclonal Sheep IgG WB AF4177 100 µg




RictorANTIBODIES


Rictoraa 1-250


RSKANTIBODIES


Phospho-RSK (S380)Pan Specific


Phospho-RSK (T573)Pan Specific


RSKPan Specificaa 171-193









RSK1RECOMBINANT & NATURAL PROTEINS


RSK1Active


ANTIBODIES


RSK1 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF992 100 µg



Phospho-RSK1 (S380) Human/Mouse/Rat 125 - 8000 pg/mL DYC892-2 2 Plates


RSK1/RSK2ANTIBODIES


Phospho-RSK1 (S221)/RSK2 (S227)


RSK2ANTIBODIES


RSK2 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1518 50 µg

RSK3ANTIBODIES


Phospho-RSK3 (S218) Human/Mouse None Polyclonal Rabbit IgG IHC, WB AF893 100 µg





RSK4ANTIBODIES


RSK4 Human None 374521 Rat IgG2A WB MAB3916 100 µg

SrcRECOMBINANT & NATURAL PROTEINS


SrcActive

Viral Solution without a carrier protein None Sf 9 3389-KS-010 10 µg

SrcActive

Human Solution without a carrier protein None E. coli 4595-KS-010 10 µg

ANTIBODIES


Phospho-Src (T419) Human None Polyclonal Rabbit IgG IHC, WB AF2685 100 µg

Src Human None 327554 Mouse IgG2A IHC MAB3807 100 µg

Srcaa 1-79


Srcaa 1-79




Phospho-Src (Y419) Human 3.9 - 250 pg/mL DYC2685-2 2 Plates


TORANTIBODIES


Phospho-TOR (S2448) Human None Polyclonal Rabbit IgG WB AF1665 100 µg

TOR Human/Mouse None 303728 Rat IgG2A IHC MAB1537 100 µg

TORaa 1223-1290 (rat)

Human/Mouse/Rat None Polyclonal Total Rabbit IgG WB AB1537 100 µg

TORaa 1720-2020 (human)

Human/Mouse/Rat None Polyclonal Goat IgG IP, WB AF15371 100 µg

ELISAs/ASSAYS


Phospho-TOR (S2448)Surveyor IC ELISA

Human � Cell Lysate N/A 156-10000 pg/mL SUV1665 1 Kit



Phospho-TOR (S2448) Human 156 - 10000 pg/mL DYC1665-2 2 Plates


Tyk2ANTIBODIES


Tyk2aa 2-51





NF�B Pathway



NF�B1 and NF�B2 are members of the Rel/NF�B family of tran-

scription factors that also includes RelA, c-Rel, and RelB. Rel/NF�B

members regulate the expression of genes that participate in im-

mune, apoptotic and oncogenic processes. NF�B is predominantly

localized in the cytoplasm as a complex with inhibitory I�B pro-

teins and is released and translocated to the nucleus after

phosphorylation of I�B. Both NF�B1 (105 kDa) and NF�B2

(100 kDa) are synthesized as precursor molecules that are

proteolytically cleaved to 50 and 52 kDa active subunits. NF�B ap-

pears to have contradictory functions in apoptosis and cell survival.

NF�B mediates the survival response of many signals by inhibiting

p53-dependent apoptosis, and up-regulating anti-apoptotic mem-

bers of the Bcl-2 family and caspase inhibitors, such as XIAP and

FLIP. In contrast, NF�B is also activated by apoptotic stimuli in-

volved in DNA damage and mediates up-regulation of

pro-apoptotic genes such as TRAIL R2/DR5, Fas, and Fas ligand.

For more information on NFκB-related products, please visit our

website at www.RnDSystems.com/go/NFkB

NF�B Promotes the Expression of Pro- and Anti-apoptotic Genes. In addition to the recruitment of FADD and Pro-caspase-8, -10, the TRADD adaptor protein can recruit

TRAF-2 leading to the activation of the NF�B transcription factor. NF�B activates the expression of both pro- and anti-apoptotic genes.

Detection of NF�B2 in Daudi Cells. Exponentially growing human Burkitt's lymphoma Daudi cells were either unstimulated (-)

or stimulated (+) with recombinant human CD40 Ligand (Catalog # 617-CL) for 4 hours. NF�B2 was detected in cytoplasmic and

nuclear extracts by Western blot analysis using anti-human NF�B2 monoclonal antibody (Catalog # MAB28881). A whole cell ex-

tract from Burkitt's lymphoma-derived Raji cells is shown as a control in lane 5.

NF�B

c-RelANTIBODIES


c-Rel Human None 321422 Rat IgG2A IHC, WB MAB4606 50 µg

c-Relaa 1-588

Human/Mouse None Polyclonal Goat IgG IHC, WB AF2699 100 µg

c-Relaa 1-588

Mouse Biotin 290512 Rat IgG2A IHC BAM2699 100 µg

c-Relaa 1-588


CARMA1/CARD11ANTIBODIES


CARMA1/CARD11aa 256-435


I�B-�ANTIBODIES


I�B-� Human None 417208 Mouse IgG1 WB MAB4299 50 µg

Phospho-I�B-� (S32/S36) Human None Polyclonal Rabbit IgG WB AF4809 100 µg

I�B-� Human/Mouse None Polyclonal Sheep IgG WB AF4299 100 µg

I�B-�ANTIBODIES


I�B-� Human/Rat None 347428 Mouse IgG1 WB MAB3425 100 µg

I�B-�ANTIBODIES


I�B-�aa 141-500


I�B-� Mouse None Polyclonal Sheep IgG WB AF4637 100 µg

IKK�ANTIBODIES


IKK�aa 375-745

Human/Mouse/Rat None Polyclonal Sheep IgG IHC, WB AF3768 100 µg

IKK�ANTIBODIES


IKK�aa 374-756





IKK�ANTIBODIES


IKK� Human None Polyclonal Goat IgG IHC, WB AF3199 100 µg

IKK� Human/Mouse/Rat None 346909 Mouse IgG1 IHC, WB MAB3199 100 µg

IKKANTIBODIES


IKK Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF2684 100 µg

IKK Human/Mouse/Rat None Polyclonal Sheep IgG WB AF4365 100 µg

NF�B1ANTIBODIES


NF�B1aa 317-437 (human)



Human/Mouse None 285412 Mouse IgG1 WB MAB2697 100 µg

NF�B2ANTIBODIES


NF�B2aa 1-447

Human None 291319 Mouse IgG1 IHC, WB MAB28881 100 µg

Pin1RECOMBINANT & NATURAL PROTEINS


Pin1WW Domainaa 2-163HRP-conjugated


ANTIBODIES


Pin1aa 2-163 (human)

Human/Mouse None 257417 Mouse IgG2B IHC, IP, WB MAB2294 100 µg

RelBANTIBODIES


RelBaa 382-579

Human None 315206 Mouse IgG2B IHC, WB MAB2698 100 µg

SUMO1ANTIBODIES


SUMO1 Human None Polyclonal Goat IgG WB AF3289 100 µg




NF�B

SUMO2/3/4ANTIBODIES


SUMO2/3/4 Human/Mouse None Polyclonal Goat IgG WB AF3020 100 µg

SUMO3ANTIBODIES


SUMO3 Human/Mouse None Polyclonal Goat IgG WB AF2959 100 µg

UbiquitinRECOMBINANT & NATURAL PROTEINS


Ubiquitin Human Solution without a carrier protein None E. coli 701-UB-025 25 µg

ANTIBODIES


Ubiquitin/Ubiquitin+1 Human None 83406 Mouse IgG2B IHC, WB MAB701 100 µg

Ubiquitin+1RECOMBINANT & NATURAL PROTEINS


Ubiquitin+1 Human Solution without a carrier protein None E. coli 703-UB-025 25 µg

ANTIBODIES


Ubiquitin+1 Human None Polyclonal Rabbit IgG WB AF703 100 µg

Ubiquitin+1 Human Biotin 83426 Mouse IgG2B ELISA(Det), WB BAM7032 250 µg

Ubiquitin+1 Human None 82001 Mouse IgG2A IHC, WB MAB703 100 µg

Ubiquitin+1 Human None 83456 Mouse IgG2B ELISA(Cap), WB MAB7031 500 µg



Ubiquitin+1 Human 125 - 8000 pg/mL DY703 15 Plates





ss



p53 is a Key Regulator in Determining the Fate of a Cell following DNA Damage. Under normal conditions, p53 is ubiquitinated by MDM2 and rapidly degraded by the

proteasome. When DNA is damaged, p53 phosphorylation stabilizes the protein by preventing its interaction with MDM2. Phosphorylated p53 can promote cell cycle arrest

and DNA repair, or apoptotic signaling pathways.

The p53 tumor suppressor protein is a multi-functional transcrip-

tion factor that regulates cellular processes affecting proliferation,

cell cycle checkpoints, and apoptosis. Binding of MDM2 regulates

p53 activity by targeting p53 for degradation and inhibits

p53-induced cell cycle arrest and apoptosis. In response to

genotoxic stress signals or DNA damage, p53 is phosphorylated, in-

hibiting its interaction with MDM2. p53 can cause cell cycle arrest

to allow for DNA damage repair or can cause the cell to undergo

apoptosis if the damage cannot be repaired. In addition, p53 has

been shown to trigger apoptotic pathways independent of its ac-

tivity as a transcription factor.

For more information on p53-related products, please visit our website

at www.RnDSystems.com/go/p53

Detection of Phosphorylated p53 in CPT-treated

Human MCF-7 Cells. Human MCF-7 breast cancer cells

were left untreated or treated with camptothecin (CPT;

1 µM) for the indicated times. A portion of the six hour

CPT-treated sample was subsequently treated with

�-phosphatase (�-PPase). Cell lysates were assessed for

p53 phosphorylation on serine 46 using the human

Phospho-p53 (S46) DuoSet® IC ELISA Development System

(Catalog # DYC1489; histogram). The same lysates were

also analyzed by Western blot (inset) using anti-human

Phospho-p53 (S46) polyclonal antibody (Catalog #

AF1489) and HRP-conjugated anti-human/mouse/rat

p53 polyclonal antibody (Catalog # HAF1355).

Dete c ti o n o f Phos ph o r ylate d p53 (S15) i n

CPT- treate d Os teo s arco ma Ce lls. Human

osteosarcoma cells were treated with camptothecin

(1µM; 4 hours). p53 phosphorylation on S15 was de-

tected using anti-human Phospho-p53 (S15) polyclonal

antibody (Catalog # AF1043). Cells were stained with a

FITC-conjugated secondary antibody (green) and

counterstained with propidium iodide (red).

Detection of p53 Binding to the p21 Promoter by

Chromatin Immunoprecipitation. Human MCF-7

breast cancer cells were treated with camptothecin

(300nM), fixed, and lysed. p53 binding to the p21 pro-

moter was assessed using the p53 ExactaChIP™

Chromatin IP Kit (Catalog # ECP1355). Briefly, cell lysates

were incubated with biotinylated anti-human p53

polyclonal antibody or biotinylated anti-goat IgG

polyclonal antibody (both provided in the kit) followed

by MagCellect Streptavidin Ferrofluid (Catalog #

MAG999) . DNA was puri f ied from the

immunoprecipitates and the p21 promoter was de-

tected by standard PCR .

p53 Pathway

p53 Pathway

53BP1ANTIBODIES


53BP1 Human None Polyclonal Goat IgG WB AF1877 100 µg

Phospho-53BP1 (S25) Human None Polyclonal Rabbit IgG WB AF3405 100 µg

ATMANTIBODIES


ATM Human None 245403 Mouse IgG3 WB MAB2290 100 µg

Phospho-ATM (S1981) Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1655 100 µg



Phospho-ATM (S1981) Human 3.12 - 200 ng/mL DYC1655-2 2 Plates


CBPANTIBODIES


CBP Human/Mouse/Rat None 257003 Mouse IgG2B IHC, WB MAB2676 100 µg

MDM2ANTIBODIES


MDM2aa 3-492

Human None 197702 Mouse IgG2B IP MAB1244 100 µg

MDM2 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1244 100 µg

MDM2 Human/Mouse/Rat Biotin Polyclonal Rabbit IgG WB BAF1244 50 µg

p53ANTIBODIES


p53 Human APC 184727 Mouse IgG2B FC IC13551A 100 Tests

Phospho-p53 (S15) Human PE 261352 Mouse IgG1 FC IC1839P 100 Tests

Phospho-p53 (S15) Human None Polyclonal Rabbit IgG IHC, IP, WB AF1043 100 µg

Phospho-p53 (S15) Human None 261352 Mouse IgG1 WB MAB1839 100 µg

Phospho-p53 (S15) Human None 261366 Mouse IgG1 IHC MAB18391 100 µg

Phospho-p53 (S20) Human None Polyclonal Rabbit IgG WB AF2286 100 µg



Phospho-p53 (S46) Human None Polyclonal Rabbit IgG IHC, WB AF1489 100 µg

p53 Human/Mouse/Rat None Polyclonal Goat IgG IP, WB AF1355 100 µg

p53 Human/Mouse/Rat Biotin Polyclonal Goat IgG WB BAF1355 50 µg

p53 Human/Mouse/Rat Agarose Polyclonal Goat IgG IP GAF1355 250 µL

p53 Human/Mouse/Rat HRP Polyclonal Goat IgG WB HAF1355 100 µL

p53 Human/Mouse/Rat None 184721 Mouse IgG2B IP, WB MAB1355 100 µg

p53 Human/Mouse/Rat None 184727 Mouse IgG2B FC MAB13551 100 µg

p53 Mouse None 197643 Rat IgG2B IP MAB1746 100 µg

Phospho-p53 (S18) Mouse None Polyclonal Rabbit IgG WB AF2887 100 µg




p53 continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS & TRANSCRIPTION FACTOR ACTIVITY ASSAYS


p53Total Protein


Phospho-p53 (S15) Human 312 - 20000 pg/mL DYC1839-2 2 Plates


P53Transcription Factor Assay

Human/Mouse N/A DYC1355-2 2 Plates

p53Total Protein

Mouse 312 - 20000 pg/mL DYC1746-2 2 Plates


EXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS


p53 Human ECP1355 1 Kit

PRIMER PAIRS


p53 Human/Rat RDP-144-025 25 Tests

p53R2ANTIBODIES


p53R2aa 1-180


p300ANTIBODIES


p300aa 2283-2414





Pin1RECOMBINANT & NATURAL PROTEINS


Pin1WW Domainaa 2-163HRP-conjugated


ANTIBODIES


Pin1aa 2-163

Human/Mouse None 257417 Mouse IgG2B IHC, IP, WB MAB2294 100 µg

RTVP-1ANTIBODIES


RTVP-1 Mouse None Polyclonal Goat IgG WB AF4468 100 µg




p53 Pathway

WISP-1/CCN4RECOMBINANT & NATURAL PROTEINS


WISP-1/CCN4 Human Lyophilized without a carrier protein None NS0 1627-WS-050 50 µg

WISP-1/CCN4 Mouse Lyophilized without a carrier protein None NS0 1680-WS-050 50 µg

ANTIBODIES


WISP-1/CCN4 Human None Polyclonal Goat IgG IHC, WB AF1627 100 µg

WISP-1/CCN4 Human Biotin Polyclonal Goat IgG WB BAF1627 50 µg

WISP-1/CCN4 Human None 213611 Mouse IgG2A WB MAB1627 500 µg

WISP-1/CCN4 Mouse None Polyclonal Sheep IgG IHC, WB AF1680 100 µg

WISP-1/CCN4 Mouse Biotin Polyclonal Sheep IgG IHC, WB BAF1680 50 µg

WISP-1/CCN4 Mouse None 214203 Rat IgG2B WB MAB1680 500 µg






In addition to NF�B and p53, the STAT family of transcription fac-

tors and Mad, Max, and c-Myc are also involved in regulating

proliferative and pro-apoptotic gene expression. The STAT family

of transcription factors is activated by intracellular kinases such as

Jak (1-3), TyK2, and Src. Upon activation, STATs are free to hetero-

or homo-dimerize and translocate to the nucleus where they pro-

mote the expression of Bcl-2 and Bcl-xL. Mad, Max, and c-Myc

belong to the basic helix-loop-helix/leucine zipper family of tran-

scription factors. Both c-Myc and Mad form heterodimers with

Max to regulate transcription. Mad-Max heterodimers are associated

with transcriptional repression and cellular differentiation, while

Myc-Max heterodimers are associated with transcriptional activa-

tion and cellular proliferation.

For more information on products related to Transcription Factors

i n v o l v e d i n a p o p t o s i s , p l e a s e v i s i t o u r w e b s i t e a t

www.RnDSystems.com/go/ApoptosisTxn

Apoptosis Transcription Factors

Other Transcription Factors Regulating Pro- and Anti-Apoptotic Signaling

Active STAT1 p91 in Human U937 Cells. Human monocytic U937

cells were treated with 100 ng/mL recombinant human IFN-� (Cata-

log # 285-IF) for the indicated times. A. Nuclear extracts were

prepared and assayed using the human/mouse Active STAT1 p91

DuoSet® IC Transcription Factor Assay (Catalog # DYC1490). B. The

same extracts (20 µg) were analyzed by Western blot. Total STAT p91

was immunoprecipitated using anti-human/mouse STAT1 p91

polyclonal antibody (Catalog # PAF-ST1) and immunoblotted using

anti-rabbit Phospho-STAT1 (Y701) polyclonal antibody (Catalog #

AF2894). C. EMSA was performed by incubating the same extracts

with 30 fmol biotinylated double-stranded oligonucleotide followed

by electrophoresis on a 5% non-denaturing polyacrylamide gel.

Detection of STAT5 Binding to the Bcl-x Pro-

moter by Chromatin Immunoprecipitation.

Mouse lymphoid Baf/3 cells were grown in the ab-

sence (-) or presence (+) of IL-3 , fixed, and lysed.

STAT5 binding to the Bcl-x promoter was assessed

using the STAT5 ExactaChIP™ Chromatin IP Kit

(Catalog # ECP2168). Briefly, immunoprecipitation

was performed by incubating cell lysates with

biotinylated anti-human/mouse STAT5 polyclonal

antibody or biotinylated anti-rabbit IgG polyclonal

antibody (both provided in the kit) followed by

MagCellect Streptavidin Ferrofluid (Catalog #

MAG999) . DNA was puri f ied from the

immunoprecipitates and the Bcl-x promoter was

detected by standard PCR. Lane 1 = DNA marker.

Intracellular Detection of STAT3 in Human

Jurkat Cells by Flow Cytometry. Human Jurkat

T cells were stained with PE-conjugated anti-hu-

man STAT3 monoclonal antibody (Catalog #

IC1799P; filled histogram) or with PE-conjugated

mouse IgG2B isotype control antibody (Catalog #

IC0041P; open histogram).

CBPANTIBODIES


CBP Human/Mouse/Rat None 257003 Mouse IgG2B IHC, WB MAB2676 100 µg

CREBANTIBODIES


Phospho-CREB (S133) None Polyclonal Rabbit IgG WB AF2510 100 µg

Human CREBaa 136 - 341


Human CREBaa 136 - 341

Mouse None 321120 Mouse IgG1 IHC MAB2989 100 µg



Phospho-CREB (S133) Human/Mouse/Rat 31.25-2000 pg/mL DYC2510-2 2 Plates

*DuoSet IC Kits are also available in 5 plate packs and 15 plate Economy Packs.

EXACTAChIP CHROMATIN IP KITS


CREB Human ECP2989 1 Kit

FosB/G0S3ANTIBODIES


FosB/G0S3 Human None Polyclonal Goat IgG IHC, WB AF2214 100 µg

FosB/G0S3 Human Biotin Polyclonal Goat IgG WB BAF2214 50 µg

FosB/G0S3 Human/Mouse None 269928 Mouse IgG2B WB MAB2214 100 µg

JunBANTIBODIES


JunB Human None Polyclonal Sheep IgG WB AF4456 100 µg

MAD1L1ANTIBODIES


MAD1L1aa 1-350


MaxANTIBODIES


Maxaa 37-141





c-MycANTIBODIES


c-Mycaa 66-201


c-Myc Human Biotin Polyclonal Goat IgG WB BAF3696 50 µg



c-Myc Human ECP3696 1 Kit

NF�B1ANTIBODIES





Human/Mouse None 285412 Mouse IgG1 WB MAB2697 100 µg

NF�B2ANTIBODIES


NF�B2aa 1-447

Human None 291319 Mouse IgG1 IHC, WB MAB28881 100 µg

p53ANTIBODIES


p53 Human APC 184727 Mouse IgG2B FC IC13551A 100 Tests

Phospho-p53 (S15) Human PE 261352 Mouse IgG1 FC IC1839P 100 Tests

Phospho-p53 (S15) Human None Polyclonal Rabbit IgG IHC, IP, WB AF1043 100 µg

Phospho-p53 (S15) Human None 261352 Mouse IgG1 WB MAB1839 100 µg

Phospho-p53 (S15) Human None 261366 Mouse IgG1 IHC MAB18391 100 µg




Phospho-p53 (S46) Human None Polyclonal Rabbit IgG IHC, WB AF1489 100 µg

p53 Human/Mouse/Rat None Polyclonal Goat IgG IP, WB AF1355 100 µg

p53 Human/Mouse/Rat Biotin Polyclonal Goat IgG WB BAF1355 50 µg

p53 Human/Mouse/Rat Agarose Polyclonal Goat IgG IP GAF1355 250 µL

p53 Human/Mouse/Rat HRP Polyclonal Goat IgG WB HAF1355 100 µL

p53 Human/Mouse/Rat None 184721 Mouse IgG2B IP, WB MAB1355 100 µg

p53 Human/Mouse/Rat None 184727 Mouse IgG2B FC MAB13551 100 µg

p53 Mouse None 197643 Rat IgG2B IP MAB1746 100 µg

Phospho-p53 (S18) Mouse None Polyclonal Rabbit IgG WB AF2887 100 µg





p53 continuedDUOSET IC TRANSCRIPTION FACTOR ELISAS AND ACTIVITY ASSAYS


p53Total Protein




p53Transcription FactorActivity Assay


p53Total Protein

Mouse 312 - 20000 pg/mL DYC1746-2 2 Plates



ANALYTE SPECIES CATALOG SIZE


PRIMER PAIRS


p53 Human/Rat RDP-144-025 25 Tests

c-RelANTIBODIES


c-Rel Human None 321422 Rat IgG2A IHC, WB MAB4606 50 µg

c-Relaa 1-588

Human/Mouse None Polyclonal Goat IgG IHC, WB AF2699 100 µg

c-Relaa 1-588

Mouse Biotin 290512 Rat IgG2A IHC BAM2699 100 µg

c-Relaa 1-588


RelBANTIBODIES


RelBaa 382-579

Human None 315206 Mouse IgG2B IHC, WB MAB2698 100 µg

STAT1ANTIBODIES


STAT1aa 686-750 (human)

Human Biotin Polyclonal Goat IgG ELISA(Det) BAF1490 50 µg

Phospho-STAT1 (Y701) Human/Mouse None Polyclonal Rabbit IgG FC, WB AF2894 50 µg


Human/Mouse None Polyclonal Goat IgG IP, WB PAF-ST1 100 µg

DUOSET IC TRANSCRIPTION FACTOR ACTIVITY ASSAYS


STAT1Transcription FactorActivity Assay







STAT2ANTIBODIES


Phospho-STAT2 Human Fluorescein Polyclonal Rabbit IgG FC IC2890F 100 Tests

STAT2aa 679-851

Human Biotin Polyclonal Goat IgG ELISA(Det) BAF1666 50 µg

STAT2aa 679-851

Human None Polyclonal Goat IgG IP, WB PAF-ST2 100 µg

Phospho-STAT2 (Y689) Human/Mouse None Polyclonal Rabbit IgG FC, WB AF2890 50 µg






STAT3ANTIBODIES


Phospho-STAT3 (Y705) Human None Polyclonal Rabbit IgG WB AF4607 100 µg

STAT3 Human PE 232209 Mouse IgG2B FC IC1799P 100 Tests

STAT3aa 1-175


STAT3aa 1-175

Human Fluorescein 232209 Mouse IgG2B FC IC1799F 100 Tests

STAT3aa 1-175

Human None 232209 Mouse IgG2B FC, IHC, IP, WB MAB1799 100 µg

STAT3aa 688-770 (mouse)

Human/Mouse/Rat None Polyclonal Goat IgG IHC, IP AF1799 100 µg



Phospho-STAT3 (Y705) Human/Mouse N/A DYC4607-2 2 Plates




STAT3 Human/Mouse ECP1799 1 Kit

STAT4ANTIBODIES


Phospho-STAT4 (Y693) Human None Polyclonal Rabbit IgG WB AF4319 100 µg


Human/Mouse Biotin Polyclonal Goat IgG ELISA(Det) BAF1574 50 µg


Human/Mouse None Polyclonal Goat IgG IP, WB PAF-ST4 100 µg


Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST4 200 µL






STAT5a/bANTIBODIES


Phospho-STAT5a/b (Y699) Human None Polyclonal Rabbit IgG WB AF4190 100 µg

STAT5a/baa 219-390 (human)





STAT5a/b continuedEXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS


STAT5a/b Human/Mouse ECP2168 1 Kit

STAT5aANTIBODIES


STAT5a Human Fluorescein 251610 Mouse IgG1 FC IC21741F 100 Tests

STAT5aaa 774-794

Human PE 251610 Mouse IgG1 FC IC21741P 100 Tests

STAT5aaa 774-794

Human None 251610 Mouse IgG1 FC, IHC MAB21741 100 µg

STAT5aaa 774-794 (human)

Human/Mouse None 251619 Mouse IgG3 IHC, WB MAB2174 100 µg

STAT5aaa 775-794 (mouse)

Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST5A 200 µL

STAT5bANTIBODIES


STAT5baa 777-787

Human None 389215 Mouse IgG2B FC, WB MAB1584 100 µg

STAT5baa 769-782 (human)

Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST5B 200 µL

STAT5baa 777-787 (human)

Human/Mouse None Polyclonal Rabbit IgG FC, IP, WB AF1584 50 µg

STAT6ANTIBODIES


Phospho-STAT6 (Y641) Human None Polyclonal Rabbit IgG FC, WB AF3717 50 µg

Phospho-STAT6 (Y641) Human APC Polyclonal Rabbit IgG FC IC3717A 100 Tests

Phospho-STAT6 (Y641) Human Fluorescein Polyclonal Rabbit IgG FC IC3717F 100 Tests

Phospho-STAT6 (Y641) Human PE Polyclonal Rabbit IgG FC IC3717P 100 Tests

STAT6aa 342-640

Human APC 253906 Mouse IgG2A FC IC2167A 100 Tests

STAT6aa 342-640

Human Fluorescein 253906 Mouse IgG2A FC IC2167F 100 Tests

STAT6aa 342-640

Human PE 253906 Mouse IgG2A FC IC2167P 100 Tests


Human None 253906 Mouse IgG2A FC, WB MAB2167 100 µg




Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST6 200 µL


Human/Mouse/Rat None Polyclonal Goat IgG FC, WB AF2167 100 µg

STAT6aa 627-837





Mouse N/A DYC2169-2 2 Plates


CELL-BASED ASSAYS


Phospho-STAT6 (Y641)Cell-Based ELISA








Cytolytic Granule Proteins

Cytotoxic cells, such as cytotoxic T lymphocytes (CTLs) and natural

killer (NK) cells, play critical roles in the immune response to viruses

and cellular transformation. This results from their ability to recog-

nize and initiate apoptosis in affected cells. One mechanism

cytotoxic cells utilize is the release of lysosomal granules containing

proteins capable of entering the cytoplasm of the target cell and

initiating cascades of events that ultimately result in its controlled

destruction. These include the granzymes, a family of serine pro-

teases that exhibit differences in substrate specificity and

species-specific expression patterns. Several are capable of initiat-

ing apoptotic cascades by caspase-dependent and independent

mechanisms. Granulysin is a granule protein known for its actions

as an anti-microbial agent and may also have a direct cytotoxic ef-

fect on mammalian cells. Other granule-associated proteins include

additional apoptosis mediators, proteins responsible for activating

pro-enzymes, and those with putative involvement in protecting

the effector cell from self destruction (Table 1).

Protein Function

Granzymes Serine proteases with roles in caspase-dependent and-independent apoptosis

Perforin Membrane perturbing protein important for granzymeentry into target cell cytoplasm

Calreticulin Binds Perforin and may inhibit Perforin-mediateddamage to the effector cell

Cathepsin B Appears on the cell surface following degranulation andmay offer protection from Perforin-mediatedself-destruction

Cathepsin C Processes granzyme pro-enzymes

Granulysin Human membrane perturbing microbicidal protein thatcan initiate cytochrome c release and apoptosis

Serglycin Proteoglycan that non-covalently binds granzymes andmay act as a molecular chaperone

Chemokines Small proteins capable of mediating chemotaxis and/orcell activation

LAMPs Protection of lysosomal membranes from enzymaticattack or mediate fusion of phagosomes with lysosomes

WIP Cytolytic granule transport

FAS Ligand/TNFSF6 Potent apoptosis-inducing TNF superfamily member

Granzymes are structurally related serine proteases that differ in

their substrate specificity. To date, five different granzymes have

been described in humans: Granzymes A, B, H, K and M. In

mice, clear orthologues of four of these Granzymes (A, B, K and M)

can be found, and Granzyme C seems the most probable murine

orthologue of Granzyme H. The murine genome encodes several

additional orphan Granzymes (D, E, F, G, L and N). Of these, D, E,

F, and G are expressed by cytotoxic lymphocytes, but not much

else, other than their proposed specificities, is known about them.

Their ubiquitous expression in cytotoxic lymphocytes suggests

that, like the more well-characterized granzymes, these orphan

granzymes also play a role in host protection.

The pathways by which different granzymes carry out cell death are

widely varied. Table 2 shows a description of the activity of the

granzymes for which a function in cytotoxicity has been reported.

For more information on Granzyme-related products, please visit our

website at www.RnDSystems.com/go/Granzymes

Table 1. Cytolytic granule contents and their functions

Table 2. Activities and Mechanisms of Cell Death Induced by Cytotoxic Granzymes

Cytotoxic Granule Proteins

Granzyme-mediated Cell Death

Granzyme A B H/C K M

Activity Tryptase Chymase Chymase Tryptase Metase

Predicted Specificities Arg/Lys/Phe Asp/Asn/Met/Ser Phe/Leu Arg/Lys/Phe Met/Leu

Chromatin Condensation Yes Yes Yes Yes Yes

DNA Fragmentation No Yes No No No

DNA Nicks Yes No Yes Yes Not Determined

Caspase Dependent No Yes No No No

Inhibition by Bcl-2 No Yes Not Determined Yes No

Cytochrome c Release No Yes Yes No No

Mitochondrial Swelling Yes Yes Yes Yes Yes



Granzyme-mediated Cell Death Involves Caspase-dependent and Caspase-independent Pathways. Cytotoxic cells release cytolytic granules containing proteins such as

Perforin, and granzymes that can enter the cytoplasm of target cells and promote caspase-dependent, or caspase-independent cell death signaling pathways. Granzyme A in-

duces caspase-independent cell death by targeting components of the SET complex which release NM23-H1 to elicit single-stranded DNA nicks. Other Granzyme A substrates

include the core and H1 histones and the nuclear lamins. Granzyme B directly targets Pro-caspase-3 and Pro-caspase-7 to activate apoptotic cell death. In addition, Granzyme B

targets BID leading to the release of Cytochrome c from the mitochondria and the subsequent formation of the apoptosome. Granzyme B may also directly target Lamin B, PARP,

and ICAD, and other cellular substrates to promote cell death.

G ra n z ym e A in H u m a n Pe rip h e ra l B l o o d

Mononuclear Cells. Granzyme A was detected in

human peripheral blood mononuclear cells using

anti-human Granz yme A monoclonal antibody

(Catalog # MAB29051) followed by anti-mouse

NorthernLights™-557 secondary antibody (Catalog #

NL007; red). Cells were counterstained with FluoroNissl™

Green (green).

Intracellular Detection of Granzyme A in CD56+

Whole Blood Cells by Flow Cytometry. CD56+ whole

blood cells were stained with CFS-conjugated anti-hu-

man Granzyme A monoclonal antibody (Catalog #

IC29051F; filled histogram) or with CFS-conjugated

mouse IgG2B isotype control antibody (Catalog #

IC0041F; open histogram).

Detection of Granzyme B-secreting Cells by ELISpot.

Peripheral blood mononuclear cells, stimulated with

calcium ionomycin and PMA, were assessed for

Granzyme B secretion using the human Granzyme B

ELISpot Kit (Catalog # EL2906). Briefly, activated cells

were added to the ELISpot plate for 17 hours followed by

development with biotinylated anti-Granzyme B detec-

tion antibody, and alkaline phosphatase-conjugated

Streptavidin. Granzyme B-secreting cells were revealed

as purple spots in the microplate well. The inset shows a

well in which no detection antibody was added.

Granzyme ARECOMBINANT & NATURAL PROTEINS


Granzyme A Human Lyophilized without a carrier protein None NS0 2905-SE-010 10 µg

ANTIBODIES


Granzyme A Human APC 356412 Mouse IgG2B FC IC29051A 100 Tests

Granzyme A Human Fluorescein 356412 Mouse IgG2B FC IC29051F 100 Tests

Granzyme A Human PE 356412 Mouse IgG2B FC IC29051P 100 Tests

Granzyme A Human None 356422 Mouse IgG1 WB MAB2905 100 µg

Granzyme A Human None 356412 Mouse IgG2B FC, IHC, IP MAB29051 100 µg

Granzyme BRECOMBINANT & NATURAL PROTEINS


Granzyme B Human Solution without a carrier protein None NS0 2906-SE-010 10 µg

Granzyme B Mouse Solution without a carrier protein None NS0 1865-SE-010 10 µg

ANTIBODIES


Granzyme B Human None Polyclonal Goat IgG WB AF2906 100 µg

Granzyme B Human Biotin Polyclonal Goat IgG FC, WB BAF2906 50 µg

Granzyme B Human APC 351927 Mouse IgG2A FC IC2906A 100 Tests

Granzyme B Human PE 351927 Mouse IgG2A FC IC2906P 100 Tests

Granzyme B Human None 351927 Mouse IgG2A FC, IHC, WB MAB2906 100 µg

Granzyme B Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,IP, WB

AF1865 100 µg

Granzyme B Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1865 50 µg

Granzyme B Mouse None 216315 Rat IgG2A WB MAB1865 500 µg



Granzyme B Mouse 62.5 - 4000 pg/mL DY1865 15 Plates


ELISPOT KITS

ANALYTE SPECIES CATALOG # SIZE**

Granzyme B Human EL2906 1 Kit

Granzyme B Mouse EL1865 1 Kit

** ELISpot Kits are also available in 6 and 50 plate packs and development modules containing enough reagents for 5 plates, please inquire.

Granzyme DRECOMBINANT & NATURAL PROTEINS


Granzyme D Mouse Lyophilized without a carrier protein None NS0 1365-SE-010 10 µg

ANTIBODIES


Granzyme D Mouse None Polyclonal Goat IgG IHC, IP, WB AF1365 100 µg

Granzyme D Mouse Biotin Polyclonal Goat IgG WB BAF1365 50 µg

Granzyme D Mouse None 190617 Rat IgG1 WB MAB1365 500 µg




Cytotoxic Granule Proteins

Granzyme GRECOMBINANT & NATURAL PROTEINS


Granzyme G Mouse Solution without a carrier protein None Sf 21 1346-SE-010 10 µg

ANTIBODIES


Granzyme G Mouse None Polyclonal Goat IgG IP, WB AF1346 100 µg

Granzyme G Mouse Biotin Polyclonal Goat IgG WB BAF1346 50 µg

Granzyme G Mouse None 207639 Rat IgG2A WB MAB1346 500 µg

Granzyme HRECOMBINANT & NATURAL PROTEINS


Granzyme H Human Solution without a carrier protein None NS0 1377-SE-010 10 µg

ANTIBODIES


Granzyme H Human None Polyclonal Goat IgG IHC, IP, WB AF1377 100 µg

Granzyme H Human Biotin Polyclonal Goat IgG IHC, WB BAF1377 50 µg

Granzyme H Human None 185813 Mouse IgG2B WB MAB1377 500 µg




Other Apoptosis Molecules

AATKANTIBODIES


AATK Human None 456006 Mouse IgG2B FC MAB4866 100 µg

Annexin VRECOMBINANT & NATURAL PROTEINS


Annexin V Human Solution without a carrier protein Fluorescein E. coli NX50 100 Tests !

Annexin V Human Solution without a carrier protein PE E. coli NXPE 100 Tests

ANTIBODIES


Annexin V Human None Polyclonal Goat IgG WB AF399 100 µg

Annexin V Human Biotin Polyclonal Goat IgG WB BAF399 50 µg

For Annexin V apoptosis assays, see Apoptosis Detection Kits section (page 5) at the front of the catalog.

Apolipoprotein H/ApoHANTIBODIES


Apolipoprotein H/ApoH,aa 20-345


C1DANTIBODIES


C1D Human None Polyclonal Goat IgG WB AF4816 100 µg

CRF21ANTIBODIES


CRF21 Human None Polyclonal Sheep IgG WB AF5086 100 µg

CTGF/CCN2ANTIBODIES


CTGF/CCN2C-Terminus


CTGF/CCN2C-Terminus

Human Biotin Polyclonal Goat IgG WB BAF660 50 µg

CTGF/CCN2C-Terminus

Human None 88430 Mouse IgG1 IHC MAB660 500 µg

Cyr61/CCN1ANTIBODIES


Cyr61/CCN1 Human None 365108 Mouse IgG1 WB MAB4055 100 µg

Cyr61/CCN1 Mouse None Polyclonal Sheep IgG IHC, WB AF4055 100 µg

Cyr61/CCN1 Mouse None 466305 Rat IgG2B WB MAB4864 100 µg





MFG-E8RECOMBINANT & NATURAL PROTEINS


MFG-E8 Human Lyophilized without a carrier protein None NS0 2767-MF-050 50 µg

MFG-E8 Mouse Lyophilized with a carrier protein None NS0 2805-MF-050 50 µg

ANTIBODIES


MFG-E8 Human APC 278918 Mouse IgG2A FC IC27671A 100 Tests

MFG-E8 Human PE 278918 Mouse IgG2A FC IC27671P 100 Tests

MFG-E8 Human None 278901 Mouse IgG2A WB MAB2767 100 µg

MFG-E8 Human None 278918 Mouse IgG2A FC, WB MAB27671 100 µg

MFG-E8 Mouse None Polyclonal Goat IgG WB AF2805 100 µg

MFG-E8 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF2805 50 µg

MFG-E8 Mouse None 340614 Rat IgG2A ELISA(Cap), WB MAB2805 100 µg



MFG-E8 Mouse 31.25 - 2000 pg/mL DY2805 15 Plates


OAS2ANTIBODIES


OAS2 Human None HLS56/3 Mouse IgG2A IHC, IP MAB1925 100 µg

PARPANTIBODIES


PARPaa 71-329 (mouse)

Human/Mouse None Polyclonal Goat IgG IP, WB AF-600-NA 100 µg


Human/Mouse Biotin Polyclonal Goat IgG WB BAF600 50 µg



PARP Multi-species None C2-10 Mouse IgG1 IHC, WB 4338-MC-50 50 µg

PBRANTIBODIES


PBR Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB 6361-PC-100 100 µL

PBR Mouse/Rat None Polyclonal Rabbit IgG IHC, WB 6362-PC-100 100 µL

PoriminANTIBODIES


Porimin Human None Polyclonal Total Goat IgG FC, WB AB3010 1 mg

Porimin Human None 297617 Mouse IgG2B FC, WB MAB3010 100 µg




ss

PSRANTIBODIES


PSR Mouse None Polyclonal Goat IgG WB AF1994 100 µg

PSR Mouse Biotin Polyclonal Goat IgG WB BAF1994 50 µg

Thymosin �1/PTMARECOMBINANT & NATURAL PROTEINS


Thymosin �1/PTMA Human Solution without a carrier protein None Synthetic 11976-1 500 µg

Transglutaminase 2RECOMBINANT & NATURAL PROTEINS


Transglutaminase 2 Human Solution without a carrier protein None Sf 21 4376-TG-050 50 µg







4-Amino-1,8-Naphthalimide .................................. 14

6(5H)-Phenanthridinone .......................................... 14

6-Biotin-17-NAD.......................................................... 14

14-3-3 .............................................................................. 59

14-3-3b ........................................................................... 59

14-3-3e............................................................................ 59

14-3-3h ........................................................................... 59

14-3-3s ........................................................................... 60

14-3-3q ........................................................................... 60

14-3-3z ........................................................................... 60

4-1BB/TNFRSF9 ........................................................... 29

4-1BB Ligand/TNFSF9 ............................................... 29

4-Hydroxynonenal ..................................................... 75

53BP1 ............................................................................102

aA Crystallin/CRYAA .................................................. 80

AATK ..............................................................................115

aB Crystallin/CRYAB .................................................. 80

AIF .................................................................................... 71

Akt .................................................................................... 84

Akt1 ...........................................................................84-85

Akt2 ................................................................................. 85

Akt3 ................................................................................. 85

Annexin V ....................................................................115

Annexin V Assay .............................................................5

APAF-1 ............................................................................ 19

Apolipoprotein H/ApoH ........................................115

ApoStat Detection Kit ..................................................6

APRIL/TNFSF13 ............................................................ 30

ARC .................................................................................. 19

ASC .................................................................................. 19

ASK1 ................................................................................ 85

ATM ................................................................................102

Bad ................................................................................... 65

BAFF/TNFSF13B .......................................................... 30

BAFF R/TNFRSF13C .................................................... 31

Bag-1 ............................................................................... 65

BAK .................................................................................. 65

Bax .............................................................................65-66

Bcl-10 .............................................................................. 66

Bcl-2 ................................................................................. 66

Bcl-2 related protein A1 ........................................... 67

Bcl-w ................................................................................ 67

Bcl-x ................................................................................. 67

Bcl-xL ..........................................................................67-68

BCMA/TNFRSF17 ........................................................ 31

Benzamide .................................................................... 14

BID .................................................................................... 68

Bik-like Killer Protein ................................................. 68

BIM ................................................................................... 68

BNIP3............................................................................... 68

BNIP3L ............................................................................ 69

BOK .................................................................................. 69

C1D ................................................................................115

Calcein AM .......................................................................4

CaMKKa ......................................................................... 85

Carboxymethyl Lysine .............................................. 74

CARMA1/CARD11 ....................................................... 98

Caspase .......................................................................... 20

Caspase-1 ...................................................................... 20

Caspase-2 ...................................................................... 20

Caspase-3 ................................................................20-21

Caspase-4 ...................................................................... 21

Caspase-6 ...................................................................... 21

Caspase-7 ...................................................................... 21

Caspase-8 ...................................................................... 22

Caspase-9 ...................................................................... 22

Caspase-10 ..............................................................22-23

Caspase-12 .................................................................... 23

Caspase-13 .................................................................... 23

Catalase .......................................................................... 74

CBP ........................................................................102, 106

CD27/TNFRSF7 ............................................................ 32

CD27 Ligand/TNFSF7 ................................................ 32

CD2AP............................................................................. 60

CD30/TNFRSF8 ............................................................ 33

CD30 Ligand/TNFSF8 ..........................................33-34

CD40/TNFRSF5 ............................................................ 34

CD40 Ligand/TNFSF5 ................................................ 35

Cell Culture Control Slides ....................................... 14

cIAP .................................................................................. 25

cIAP-1/HIAP-2 .............................................................. 25

cIAP-2/HIAP-1 .............................................................. 25

Clusterin ......................................................................... 80

Cobalt Cation ............................................................... 14

CometAssays ...................................................................8

CometSlide ................................................................... 14

CRADD ............................................................................ 58

CREB ..............................................................................106

CRF21 ............................................................................115

Crk .................................................................................... 58

CRMB ............................................................................... 35

CTGF/CCN2 .................................................................115

Cyr61/CCN1 ................................................................115

Cytochrome c .........................................................71-72

Cytonin ........................................................................... 14

DAXX ............................................................................... 59

DcR3/TNFRSF6B .......................................................... 36

DcTRAIL R1/TNFRSF23 .............................................. 36

DcTRAIL R2/TNFRSF22 .............................................. 36

DePsipher Assay .............................................................6

DFF45/ICAD .................................................................. 60

DNA Laddering Detection Kits .................................9

DR3/TNFRSF25 ............................................................ 37

DR6/TNFRSF21 ............................................................ 37

EDA ............................................................................37-38

EDA-A2 ........................................................................... 38

EDAR ............................................................................... 38

eNOS .........................................................................75-76

ERK1 ................................................................................ 86

ERK1/ERK2 ...............................................................85-86

ERK2 ..........................................................................86-87

FADD ............................................................................... 59

Fas/TNFRSF6 ...........................................................38-39

Fas Ligand/TNFSF6..................................................... 40

FLIP ............................................................................23, 60

FosB/G0S3 ...................................................................106

GbL................................................................................... 87

GITR/TNFRSF18 ........................................................... 41

GITR Ligand/TNFSF18 .........................................41-42

Glutaredoxin 1 ............................................................. 74

Glutathione Peroxidase 1 ........................................ 74

Glutathione Peroxidase 3 ........................................ 74

Glyoxalase I ................................................................... 74

Granzyme A ................................................................113

Granzyme B ................................................................113

Granzyme D ................................................................113

Granzyme G ................................................................114

Granzyme H ................................................................114

GRP75/HSPA9B ............................................................ 80

GRP78/HSPA5............................................................... 80

GSK-3a ........................................................................... 87

GSK-3a/b ....................................................................... 87

GSK-3b ............................................................................ 87

HO-1/HMOX1/HSP32 ..........................................74, 80

HO-2/HMOX2 ............................................................... 74

Holocytochrome c...................................................... 72

Hrk.................................................................................... 69

HSP10/EPF..................................................................... 81

HSP20/HSPB6 ............................................................... 81

HSP27.............................................................................. 81

HSP40/DNAJB1 ............................................................ 81

HSP60.............................................................................. 81

HSP70.............................................................................. 82

HSP90.............................................................................. 82

Index


119

HSPA8/HSC71............................................................... 82

HSPH1 ............................................................................. 82

HTRA2/Omi .............................................................25, 72

HVEM/TNFRSF14 ......................................................... 42

IkB-a ................................................................................ 98

IkB-b ................................................................................ 98

IkB-e................................................................................. 98

IKKa ................................................................................. 98

IKKb.................................................................................. 98

IKKe .................................................................................. 99

IKKg .................................................................................. 99

iNOS................................................................................. 76

Jak1 .................................................................................. 88

Jak2 .................................................................................. 88

Jak3 .................................................................................. 88

JNK ................................................................................... 88

JNK1 ..........................................................................88-89

JNK1/JNK2 ..................................................................... 88

JNK2 ................................................................................ 89

JunB ...............................................................................106

LIGHT/TNFSF14 .....................................................42-43

Livin ...........................................................................25-26

LMTK2 ............................................................................. 89

Lymphotoxin ................................................................ 43

Lymphotoxin b/TNFSF3 ........................................... 43

Lymphotoxin b R/TNFRSF3 ..................................... 43

MAD1L1 .......................................................................106

Magnesium Cation ..................................................... 14

Manganese Cation ..................................................... 14

Max ................................................................................106

Mcl-1 ............................................................................... 69

MDM2 ...........................................................................102

MEK1 ............................................................................... 89

MEK1/MEK2 ............................................................89-90

MEK2 ............................................................................... 90

MFG-E8 .........................................................................116

MKK3 ............................................................................... 90

MKK3/MKK6 .................................................................. 90

MKK4 ............................................................................... 90

MKK6 ............................................................................... 90

MKK7 ............................................................................... 90

MLK3 ............................................................................... 91

MLK4a ............................................................................ 91

MSK1 ............................................................................... 91

MSK1/MSK2 .................................................................. 91

MSK2 ............................................................................... 91

MST1/STK4 .................................................................... 91

MST2/STK3 .................................................................... 91

MTT .....................................................................................3

c-Myc .............................................................................107

MyD88 ............................................................................ 61

Myeloperoxidase ........................................................ 75

NAIP ................................................................................. 26

NALP2 ............................................................................. 23

NeuroPore ..................................................................... 14

NFkB1 .................................................................... 99, 107

NFkB2 .................................................................... 99, 107

NGF R/TNFRSF16 ........................................................ 44

Nitric Oxide ................................................................... 75

Nitrotyrosine ................................................................ 75

nNOS ............................................................................... 76

OAS2..............................................................................116

Osteoprotegerin/TNFRSF11B ...........................44-45

OX40/TNFRSF4 ............................................................ 45

OX40 Ligand/TNFSF4 ..........................................45-46

p38 .............................................................................91-92

p38a ................................................................................ 92

p38b ................................................................................ 92

p38d ................................................................................ 92

p38g ................................................................................. 93

p53 ......................................................102-103, 107-108

p53R2 ............................................................................103

p70 S6 Kinase ............................................................... 93

p70 S6 Kinaseb ............................................................ 93

p300 ..............................................................................103

Park7/DJ-1 ..................................................................... 76

PARP ..............................................................................116

PARP Activated DNA .................................................. 14

PARP Activity Assay .......................................................7

PBR .................................................................................116

PDK-1 .............................................................................. 94

Peroxiredoxin 1 ........................................................... 76

Peroxiredoxin 2 ........................................................... 76

Peroxiredoxin 5 ........................................................... 76

Peroxiredoxin 6 ........................................................... 77

Peroxynitrite ................................................................. 14

PI 3-Kinase p85a ......................................................... 94

PI 3-Kinase p110.......................................................... 94

PI 3-Kinase p110b ....................................................... 94

PI 3-Kinase p110d ....................................................... 94

PI 3-Kinase p110g........................................................ 94

Pin1 ......................................................................... 99, 103

PKA C............................................................................... 94

PKA Ca ........................................................................... 94

PKA RIb ........................................................................... 94

Poly(ADP-ribose) Polymer/pADPr ........................ 14

PON2 ............................................................................... 77

PON3 ............................................................................... 77

Porimin .........................................................................116

Proteinase K .................................................................. 14

Proteome Profiler Antibody Arrays ................15-16

PSR .................................................................................117

RANK/TNFRSF11A ...................................................... 46

c-Rel ........................................................................ 98, 108

RelB ......................................................................... 99, 108

RELT/TNFRSF19L ......................................................... 46

Resazurin ..........................................................................4

Rictor ............................................................................... 95

RIP1 .................................................................................. 59

RSK ................................................................................... 95

RSK1 ................................................................................ 95

RSK1/RSK2 ..................................................................... 95

RSK2 ................................................................................ 95

RSK3 ................................................................................ 95

RSK4 ................................................................................ 96

RTVP-1 ..........................................................................103

SMAC/Diablo ..........................................................26, 72

SOD1/Cu-Zn SOD ....................................................... 77

SOD2/Mn-SOD ............................................................ 77

SOD3/EC-SOD .............................................................. 77

SODD/BAG4 .................................................................. 60

Src .................................................................................... 96

STAT1 .............................................................................108

STAT2 .............................................................................109

STAT3 .............................................................................109

STAT4 .............................................................................109

STAT5a ..........................................................................109

STAT5a/b .............................................................109-110

STAT5b ..........................................................................110

STAT6 .............................................................................110

Streptavidin-Fluorescein .......................................... 14

Streptavidin-HRP ........................................................ 14

SUMO1 ........................................................................... 99

SUMO2/3/4 .................................................................100

SUMO3 .........................................................................100

Superoxide Dismutase ............................................. 77

Survivin ....................................................................26-27

TACI/TNFRSF13B ......................................................... 47

TACS Blue Label Substrate ...................................... 14

TACS Red Label Substrate........................................ 14

TANK ................................................................................ 61

TdT Enzyme/Buffer .................................................... 14

TdT dNTP Mix ............................................................... 14

Thioredoxin-1 .............................................................. 77

Thioredoxin-2 .............................................................. 78

Thioredoxin-80 ............................................................ 78

Thioredoxin-like 5/TRP14 ........................................ 78

Thymosin a1/PTMA .................................................117

Tissue Control Slides ................................................. 14

TL1A/TNFSF15 ............................................................. 47

For research use only. Not for use in diagnostic procedures.

Index



TNF-a/TNFSF1A ....................................................48-50

TNF-b/TNFSF1B .....................................................50-51

TNF RI/TNFRSF1A .................................................51-52

TNF RII/TNFRSF1B ....................................................... 52

TOR .................................................................................. 96

TRADD ............................................................................ 59

TRAF-1 ............................................................................ 61

TRAF-2 ............................................................................ 61

TRAF-3 ............................................................................ 61

TRAF-4 ............................................................................ 62

TRAF-6 ............................................................................ 62

TRAIL/TNFSF10 ............................................................ 53

TRAIL R1/TNFRSF10A ................................................ 53

TRAIL R2/TNFRSF10B................................................. 54

TRAIL R3/TNFRSF10C ................................................ 54

TRAIL R4/TNFRSF10D ................................................ 55

TRAM/TICAM2 ............................................................. 62

TRANCE/RANK L/TNFSF11 .................................55-56

Transglutaminase 2 .................................................117

TROY/TNFRSF19 .......................................................... 56

TUNEL Labeling Kits ............................................10-13

TWEAK/TNFSF12 ...................................................56-57

TWEAK R/TNFRSF12 .................................................. 57

TXNDC5 .......................................................................... 78

Tyk2 ................................................................................. 96

Ubiquitin ......................................................................100

Ubiquitin+1 ................................................................100

UCP2............................................................................... 72

WISP-1/CCN4 ..............................................................104

XEDAR ............................................................................. 57

XIAP ................................................................................. 27

XTT ......................................................................................3

R&D Systems, Inc.DuoSet®

ExactaChIP™

NorthernLights™

Proteome Profiler™

R&D Systems®

Surveyor™


Trevigen, Inc.CardioTACS™

CometAssay™

DePsipher™

DermaTACS™

FlowTACS™

NeuroTACS™

PeroxyGlow™

TACS™

TACS-Blue Label™

TACS-Nuclease™

TACS-Red Label™

TACS-Sapphire™

TACS-XL®

TiterTACS™

TumorTACS™

VasoTACS™

MakTek, Inc.EpiDermT™

Vector LaboratoriesNovaRed™

Molecular ProbesFluoroNissl™

Trademark InformationThe following is a listing of trademarks used by R&D Systems, Inc. This list is subject to change at any time. Registration applications for several marks are pending and may become registered marks in the near future. Any rights not expressly granted herein are reserved.

Index continued

Antibody Application KeyAP ..........................Immunoaffinity Purification

B/N........................Blocking/Neutralizing

Adhesion Blockade

Blockade of Receptor-

Ligand Interaction

CD..........................Cell Depletion

Dot ........................Dot Blot

FA ...........................Functional Assay

ELISA Cap ............ ELISA Capture (Matched Pair)

ELISA Comp .......Competitive ELISA

ELISA Det ............ELISA Detection

(Matched Pair)

EMSA ....................Gel Supershift Assay

FC ..........................Flow Cytometry

Intracellular Staining by

Flow Cytometry

IF ............................Immunofluorescence

Immunocytochemistry

IHC ........................Immunohistochemistry

IP ...........................Immunoprecipitation

WB .........................Western Blot

R&D Systems Apoptosis Catalog

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Apoptosis Catalog - R&D Systems

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