ALTERNATIVE SPLICING OF mRNA. MECHANISMS ROLE IN GENE EXPRESION CONSEQUENCES by Michał Mlącki.
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Transcript of ALTERNATIVE SPLICING OF mRNA. MECHANISMS ROLE IN GENE EXPRESION CONSEQUENCES by Michał Mlącki.
PLAN OF THE PRESENTATION:
• ANATOMY OF THE EUCARIOTIC GENE;
• THE PROCESS OF TRANSCRIPTION;
• EVENTS AFTER TRANSCRIPTION (CAPPING, SPLICING, POLIADENYLATION);
• ALTERNATIVE SPLICING (MECHANISMS AND CONSEQUENCES OF PATTERN CHANGING)
TYPES OF RNA POLYMERASES:
• POL I – 5,8S , 18S , 28S rRNA GENES;
• POL II – ALL GENES ENCODING PROTEINS snoRNA GENES;
• POL III – tRNA GENES 5S rRNA GENES SOME snRNA GENES AND OTHER SMALL RNA GENES.
CO-TRANSCRIPTIONAL EVENTS...
„GenesVIII” B. Lewin
„The mRNA assembly line: transcription and processing mechines in the same factory” D. Bentley
COCB 2002,14:336-342
„Mechanisms of fidelity in pre-mRNA splicing” Robin ReedCurrent Opinion in Cell Biology 2000, 12:340-345
„The mRNA assembly line: transcription and processing mechines in the same factory” D. Bentley
COCB 2002,14:336-342
CONSEQUENCES (1)(GOOD SIDE)
DIFFRENT PATTERN OF SPICING:
• IN DIFFRENT TISSUES;
• IN DIFFRENT STAGES OF
DEVELOPMENT;
• SOME OTHER (e.g. : SEX DETERMINATION IN DROSOPHILA)
CONSEQUENCES (2)(BAD SIDE)
• MUTATIONS IN SEQUENCES INVOLVED IN REMOVING OF INTRONS MAY CAUSE UNPHYSIOLOGICAL SPLICING AND THEREFORE DISEASES
• MAINLY NEURODEGENERATIVE DISEASES (MYOTROIC DYSTROPHY,SPIRAL MUSCULAR ATROPY ETC.)
QUESTIONS FOR THE FUTUR:
• WHY THE ALTERNATIVE SPLICING SOMETIMES IS PHISIOLOGICAL AND SOMETIMES CAUSES DISEASES?
• HOW TO FIGHT THEM?
• HOW CAN WE USE THIS PROCESS IN GENETIC AND BIOCHEMICAL MANIPULATIONS?