Alcolyzer Plus Beer

7/22/2019 Alcolyzer Plus Beer

1/129

Reference Manual

Alcolyzer Plus Beer

Beer Analyzing SystemFirmware Version: V5.02.09


2/129


3/129

Reference Manual

Alcolyzer Plus Beer

Beer Analyzing SystemFirmware Version: V5.02.09


4/129

Anton Paar GmbH assumes no liability for technical or printing errors or omissions in this document.

Nor is any liability assumed for damages resulting from information contained in the document.

Anton Paar GmbH reserves the right to content changes. This also extends to changes to delivery

volumes or any features of delivered parts.

All rights reserved (including translation). This document, or any part of it, may not be reproduced,

changed, copied, or distributed by means of electronic systems in any form (print, photocopy, microfilm

or any other process) without prior written permission by Anton Paar GmbH.

Trademarks, registered trademarks, trade names, etc. may be used in this manual without being marked

as such. They are the property of their respective owner.

Published by Anton Paar

Printed: Anton Paar, Austria

Copyright 2009 Anton Paar GmbH, Graz, Austria

Address: Anton Paar GmbH

Anton-Paar-Str. 20

A-8054 Graz / Austria Europe

Tel: +43 316 257-0

Fax: +43 316 257-257

E-Mail: [email protected]

Web: www.anton-paar.com

Date: 06.03.2009

Document number C27IB02M.fm


5/129

C27IB02 5

Contents

1 About the Reference Manual .......................................................................................................... 9

2 Safety Instructions........................................................................................................................... 9

2.1 General Safety Instructions ..................................................................................................... 9

3 About Alcolyzer Plus Beer............................................................................................................ 11

4 Testing the Supplied Parts............................................................................................................ 11

5 Putting into Operation................................................................................................................... 14

5.1 Installing the Instruments....................................................................................................... 14

5.2 Mounting the pH Electrode .................................................................................................... 16

5.2.1 Preparing and Mounting the pH Electrode "Hamilton Polilyte Lab"........................ 16

5.2.2 Preparing and Mounting the pH Electrode "Orion model 91-63"............................ 175.3 Mounting the Hoses without Option pH/Color........................................................................ 19

5.4 Mounting the Hoses with Option pH/Color............................................................................. 20

5.5 Completing the Installation .................................................................................................... 21

5.6 Connecting a Printer or PC.................................................................................................... 24

5.6.1 Connecting Alcolyzer Plus Beer to a Printer .......................................................... 24

5.6.2 Connecting Alcolyzer Plus Beer to a PC................................................................ 24

6 Functional Components................................................................................................................ 25

6.1 Display and Keypad............................................................................................................... 25

6.1.1 Display in the Measuring Mode.............................................................................. 25

6.1.2 Soft Keys in the Measuring Mode .......................................................................... 27

6.2 Rear View .............................................................................................................................. 28

7 General Instrument Settings......................................................................................................... 29

7.1 Setting the Display Contrast .................................................................................................. 29

7.2 Setting Date and Time ........................................................................................................... 29

7.3 Configuring the Measuring System........................................................................................ 29

7.3.1 Setting the General Parameters............................................................................. 29

7.3.2 Defining the Peripheral Devices ............................................................................. 30

7.3.3 Defining the Interface Settings ............................................................................... 30

7.4 Setting a Custom Function .................................................................................................... 30

7.4.1 Setting a User-Defined Ethanol Table (in %V/V).................................................... 30

7.4.2 Setting a User-Defined Color Unit .......................................................................... 31

7.5 Selecting the Output Data...................................................................................................... 32

7.5.1 Selecting the Output Data for the Display .............................................................. 33

7.5.2 Selecting the Output Data for the Printer ............................................................... 33

7.5.3 Selecting the Output Data for the Data Memory..................................................... 33

7.6 Settings for the Sample Changer........................................................................................... 33

8 Setting the Measuring Methods.................................................................................................... 34

8.1 Description of the Measuring Methods .................................................................................. 348.2 Editing a Measuring Method.................................................................................................. 35


6/129

6 C27IB02

8.2.1 Editing a Predefined Method.................................................................................. 35

8.2.2 Editing a New Method ............................................................................................ 35

8.3 Selecting the Measuring Method........................................................................................... 37

9 SOP (Standard Operating Procedure).......................................................................................... 38

10 Checking the Adjustment Validity................................................................................................ 41

10.1 Checking Procedure without Option pH/Color....................................................................... 41

10.1.1 Performing a Checking Procedure with Water (0%V/V)......................................... 41

10.1.2 Performing a Checking Procedure with a Water/Ethanol Solution of Known

Concentration ........................................................................................................................ 43

10.2 Checking Procedure with Option pH/Color............................................................................ 44

10.2.1 Checking Procedure for the Measuring Parameter "alcohol" ................................. 44

10.2.2 Checking Procedure for the Measuring Parameter "pH"........................................ 44

10.2.3 Checking Procedure for the Measuring Parameter "color"..................................... 45

11 Adjusting the Instrument .............................................................................................................. 4511.1 Adjustment without Option pH/Color...................................................................................... 45

11.1.1 Adjustment with Water (Zero Point)........................................................................ 46

11.1.2 Adjustment with a Water/Ethanol Solution of Known Alcohol Concentration ......... 48

11.2 Adjustment with Option pH/Color........................................................................................... 49

11.2.1 Adjustment Alcohol................................................................................................. 49

11.2.2 Adjustment Color.................................................................................................... 49

11.2.2.1 Adjustment with Water (Zero) ................................................................. 49

11.2.2.2 Adjustment with a Color Standard (Tartrazine) of Known Concentration 51

11.2.3 Adjustment pH........................................................................................................ 5211.2.3.1 Automatic ................................................................................................ 53

11.2.3.2 Manual .................................................................................................... 54

11.2.3.3 Edit Buffer Data....................................................................................... 55

11.2.3.4 Sort Buffer Data ...................................................................................... 56

11.3 Density Adjustment................................................................................................................ 56

12 Measurement.................................................................................................................................. 58

12.1 Identifying the Sample and Assigning the Method................................................................. 59

12.1.1 Types of Sample Identification ............................................................................... 59

12.1.2 Sample Identification for "text + number" and "text"............................................... 60

12.1.3 Sample Identification for "list" and "# + list"............................................................ 60

12.1.3.1 Entering a Sample List ............................................................................ 60

12.1.3.2 Editing the Sample List during Operation................................................ 62

12.1.3.3 Deleting a Sample List ............................................................................ 62

12.2 Performing a Measurement without Option pH/Color ............................................................ 63

12.3 Performing a Measurement with Option pH/Color................................................................. 64

12.4 Correcting Calculated Analysis Results................................................................................. 64

13 Data Memory .................................................................................................................................. 66

14 Cleaning Routines ......................................................................................................................... 67

14.1 Cleaning the Measuring Cells without Option pH/Color......................................................... 67


7/129

C27IB02 7

14.1.1 Between Series of Measurements on the Same Day............................................. 67

14.1.2 At the End of the Day ............................................................................................. 67

14.1.3 Once a Week.......................................................................................................... 68

14.2 Cleaning the Measuring Cells with Option pH/Color.............................................................. 69

14.2.1 Storing the pH Electrode ........................................................................................ 69

14.2.2 Between Series of Measurements on the Same Day............................................. 70

14.2.3 At the End of the Day ............................................................................................. 70

14.2.4 Once a Week.......................................................................................................... 71

14.3 Cleaning after Wort Measurements....................................................................................... 72

14.4 Maintaining the pH Electrode................................................................................................. 72

14.4.1 Maintaining the Hamilton pH Electrode.................................................................. 72

14.4.2 Maintaining the Orion pH Electrode ....................................................................... 73

15 Menu Operation and Description ................................................................................................. 74

15.1 Menu Operation..................................................................................................................... 7415.1.1 Operating the Soft Keys on the Instrument ............................................................ 74

15.1.2 Using an External PC Keyboard............................................................................. 75

15.1.3 Using a Bar Code Reader ...................................................................................... 75

15.2 Menu Description and Function............................................................................................. 76

15.2.1 "adjustment" ........................................................................................................... 76

15.2.1.1 "adjust alcohol"........................................................................................ 76

15.2.1.2 "adjust pH" .............................................................................................. 77

15.2.1.3 "adjust color" ........................................................................................... 77

15.2.2 "instrument settings"............................................................................................... 7815.2.3 "method settings" ................................................................................................... 82

15.2.4 "user functions" ...................................................................................................... 83

15.2.5 "data memory"........................................................................................................ 83

15.2.6 "utilities".................................................................................................................. 84

15.2.7 "parameter calculator" ............................................................................................ 90

15.2.8 "service mode" ....................................................................................................... 91

15.2.9 "auto sampler settings"........................................................................................... 91

15.3 Entering and Changing a Password ...................................................................................... 91

15.4 Installing a New Firmware Version ........................................................................................ 92

16 Calculations.................................................................................................................................... 93

17 Filling Samples with the SF-1 Sample Filling Unit...................................................................... 97

Appendix A: Degassing Beer Samples.......................................................................................... 98

Appendix B: Available Parameters ................................................................................................ 99

B.1 Method Parameters............................................................................................. 99

B.2 Printer Protocol Parameters.............................................................................. 101

Appendix C: Status and Error Messages .................................................................................... 102

C.1 Status/Error Messages for the Alcohol Measurement....................................... 102

C.2 Status/Error Messages for the Color Measurement .......................................... 103

C.3 Status/Error Messages for the pH Measurement .............................................. 103


8/129

8 C27IB02

C.4 System Error Messages.................................................................................... 104

C.5 Other Messages................................................................................................ 104

Appendix D: Technical Data ......................................................................................................... 105

Appendix E: Interface Commands for Communication PC and Alcolyzer Plus Beer ............. 106

Appendix F: Configuration for Communication between DMA and Alcolyzer Plus Beer ...... 108

Appendix G: Firmware Versions .................................................................................................. 110

Appendix H: Wetted Parts............................................................................................................. 112

Appendix I: Material Safety Data Sheet for Tartrazine.............................................................. 113

Appendix J: Density/Concentration Table for Binary Aqueous Ethanol Solutions ................ 115

Appendix K: Menu Tree................................................................................................................. 126

Index................................................................................................................................................... 128


9/129

1 About the Reference Manual

C27IB02 9

1 About the Reference Manual

This reference manual informs you about the installation and the safe handling

and use of the instrument. Make sure that the reference manual is easily

accessible to all personnel involved with the instrument. Pay special attention to

the safety instructions and warnings in the manual and on the instrument.

Symbols in this reference manual

This reference manual features the following symbols:

2 Safety Instructions

Read the reference manual at hand before using Alcolyzer Plus Beer.

Follow all hints and instructions contained in this reference manual to ensure

the correct use and safe functioning of Alcolyzer Plus Beer.

2.1 General Safety Instructions

Liability

The reference manual at hand does not claim to address all safety issues

associated with the use of the instrument and samples. It is your

responsibility to establish health and safety practices and determine the

applicability of regulatory limitations.

Anton Paar GmbH only warrants the proper functioning of Alcolyzer Plus

Beer if no adjustments have been made to the mechanics, electronics, and

firmware.

Only use Alcolyzer Plus Beer for the purpose described in this reference

manual. Anton Paar GmbH is not liable for damages caused by incorrect use

of Alcolyzer Plus Beer.

Caution:

This symbol calls attention to the risk of instrument damage or

measurement errors. Do not proceed until the indicated conditions for

averting this threat are fully understood and met.

Information:

This symbol calls attention to any additional information of use to the

operator.


10/129

2 Safety Instructions

10 C27IB02

Installation and use

Alcolyzer Plus Beer is not an explosion-proof instrument and therefore must

not be operated in areas with risk of explosion.

The installation procedure shall only be carried out by authorized personnel

who are familiar with the installation instructions.

Do not use any accessories or wearing parts other than those supplied or

approved by Anton Paar GmbH.

Make sure all operators are trained to use the instrument safely and correctly

before starting any applicable operations.

In case of damage or malfunction, do not continue operating Alcolyzer Plus

Beer. Do not operate the instrument under conditions which could result in

damage to goods and/or injuries and loss of life.

Check Alcolyzer Plus Beer for chemical resistance to the samples and

cleaning agents.

Maintenance and service

The results delivered by Alcolyzer Plus Beer not only depend on the correct

function of the instrument, but also on various other factors. We therefore

recommend to have the results checked (e.g. plausibility tested) by skilled

personnel before consequential actions are taken based on the results.

Service and repair procedures may only be carried out by authorizedpersonnel or by Anton Paar GmbH.

Disposal

Concerning the disposal of Alcolyzer Plus Beer, observe the legal

requirements in your country.

Precautions for highly flammable samples and cleaning agents

Observe and adhere to your national safety regulations for handling the

measured samples (e.g. use of safety goggles, gloves, respiratory protection

etc.).

Only store the minimum required amount of sample, cleaning agents and

other inflammable materials near the Alcolyzer Plus Beer.

Do not spill sample/cleaning agents or leave their containers uncovered.

Immediately remove spilled sample/cleaning agents.

Make sure that the setup location is sufficiently ventilated. The environment

of Alcolyzer Plus Beer must be kept free of inflammable gases and vapors.


11/129

3 About Alcolyzer Plus Beer

C27IB02 11

Connect Alcolyzer Plus Beer to the mains via a safety switch located at a

safe distance from the instrument. In an emergency, turn off the power using

this switch instead of the power switch on Alcolyzer Plus Beer.

Supply a fire extinction unit.

Ensure the sufficient supervision of Alcolyzer Plus Beer during operation.

3 About Alcolyzer Plus Beer

Alcolyzer Plus Beer is based on a highly accurate and patented NIR

spectrometer, which measures the alcohol content of beer in the concentration

range of 0 - 12%V/V.

Optionally, Alcolyzer Plus Beer can also be equipped with a color measurement

device and a pH measurement device. The color value (EBC or ASBC value) is

determined by the measurement of light absorption. For the pH measurement, an

electrode Hamilton (Polilyte Lab) or a measuring cell Orion (model 91-63) is used.

In combination with an Anton Paar density meter DMA 4500/5000 and a sample

changer SP-1m, the automatic determination of relevant beer parameters can be

achieved.

4 Testing the Supplied PartsAlcolyzer Plus Beer was tested and packed carefully before shipment. However,

damage may occur during transport.

1. Keep the packaging material (box, foam piece, transport protection) for

possible returns and further questions from the transport and insurance

company.

2. Check the delivery for completion by comparing the supplied parts to those

noted in Table 4.1.

3. If a part is missing, contact your Anton Paar representative.

4. If a part is damaged, contact the transport company and your Anton Paar

representative.
http://-/?-http://-/?-


12/129

4 Testing the Supplied Parts

12 C27IB02

Table 4.1: Supplied Parts

Symbol Pcs. Item/Spare Part Mat. No.

1 Alcolyzer Plus Beer (without option pH/color)

or

Alcolyzer Plus Beer

with option color + pH Hamilton or

with option color + pH Orion

8921

8921

9918

12167

1 Instruction manualEnglish or

German

including a CD with the reference manual

12665

12666

1 Power cable

USA:

UK:

Europe:

52656

61865

65146

1 Accessory kit 9860

2m Hose 3 x 5 silicone / 1m

Hose 1.6 x 3.2 Tygon / 1m

50814

67540

5 Syringe 5 mL / 1pc. 63130

2 Filling nozzle Luer Alcolyzer Plus / 1pc. 7046

2 Adapter Luer " UNF / 1pc. 64792

2 Male Luer plug / 1pc. 63865


13/129

4 Testing the Supplied Parts

C27IB02 13

3 Adapter Luer cone / 1pc. 63863

2 Filling nozzle fitting Alcolyzer Plus / 1pc. 10759

2 Filling nozzle UNF " Alcolyzer Plus / 1pc. 10758

2 Tube d=2.1, D=2.4, L=20 1.4571 / 1pc. 67982

1 Needle (only delivered with SF-1) / 1pc. 67394

3m Hose 2 x 4 silicone /1m

(only delivered with SF-1)

51273

1 Cable male 9 Pin / male 9 Pin / 1pc. 2359

1

(option)

Backup Card DMA 4500/5000 Alcolyzer for

firmware versions DMA V4.xxx or higher;

Alcolyzer V5.xx / 1pc.

23913

1 Waste vessel 500 mL 6210

1 Option color + pH Hamilton 9918

1 Electrode Hamilton pH = 0-14 0-80 9466

1 Option color + pH Orion 12167

1 Electrode Orion model 91-63 12555




14/129

5 Putting into Operation

14 C27IB02


5.1 Installing the Instruments

1. Put SP-1m into operation as described in the SP-1m instruction manual.

2. Connect the power cord of SP-1m to a power terminal with ground

connection.

3. Place the density meter on SP-1m.

4. Connect the connecting power cable (SP-1m accessory, Mat. No. 66178) to

the power outlet of SP-1m and the power outlet of the DMA.

5. Mount the filling nozzle fittings as described in the SP-1m instruction manual.

6. Place Alcolyzer Plus Beer on top of the density meter.

7. Connect the power cord of Alcolyzer Plus Beer to a power terminal with

ground connection.

Delivered with both

options color + pH

1 Connection cable type K1 BNC 1m 62521

1 Buffer solution pH=4.01 Hamilton (500 mL) 125541 Buffer solution pH=7.00 Hamilton (500 mL) 12556

1 pH storage solution 500 mL Hamilton 12632

1 Color adjustment set

Color standard approx. 50 EBC 2x50 mL

Ultra pure water 0.0 EBC 3x50 mL

12627



Information:

Alcolyzer Plus Beer does not require any special installation conditions.

Conditions in the installation area should be those in a typical laboratory.

To guarantee temperature stability, do not place Alcolyzer Plus Beer

- near a heater

- near an air conditioner

- in direct sunlight.

For air humidity restrictions seeAppendix D.

Caution:

To ensure the correct functioning of the built-in ventilator, make sure the

airflow at the bottom and the rear of the instrument is not blocked.


15/129


C27IB02 15

8. Screw the filling nozzle fittings Alcolyzer Plus (accessory box) into the

openings of the measuring cell.

9. If the option pH/color is integrated, check that 2# Filling nozzle Luer

Alcolyzer Plus adapters are screwed into the openings of the pH measuring

cell (see Fig. 5 - 1).

Fig. 5 - 1 Mounting the adapters onto the pH measuring cell

10. Connect COM2 at the rear of Alcolyzer Plus Beer to COM1 at the rear of

DMA 4500/5000 using the connection cable (Mat. No. 2359).

11. If a printer is used, connect the printer interface cable to COM1 at the rear of

Alcolyzer Plus Beer.

12. Connect the interface cable of SP-1m to the S-Bus outlet of Alcolyzer Plus

Beer.

Caution:

Screw the adapters into the openings of the measuring cell hand-tight(without using any tools). Otherwise, the connections between the adapters

and the measuring cell are not leak-tight and liquid may enter the electronics

and affect operation.

Caution:

Before switching on Alcolyzer Plus Beer, check that the given voltage and

frequency (AC100 to 240 V, 50 to 60 Hz) corresponds with the local mains

voltage and mains frequency. If large voltage fluctuations are to be

expected, the use of a constant voltage source (UPS) is recommended.

The non-fused earth conductor of the power cord must be connected to

earth.


16/129


16 C27IB02

5.2 Mounting the pH Electrode

5.2.1 Preparing and Mounting the pH Electrode "Hamilton

Polilyte Lab"

Preparing

1. Leave the pH electrode in an upright position for at least one hour.

2. Carefully remove the watering cap.

3. Flush the electrode with distilled water.

4. Check the inside of the electrode for unwanted air bubbles. Shaking the

electrode like a clinical thermometer will cause any bubbles to rise to the top.

Mounting

1. Loosen the protective tube by twisting it counterclockwise (see Fig. 5 - 2).

Fig. 5 - 2 Loosening the protective tube

2. Screw the connecting cable (Mat. No. 62521) to the rear end of the pH

electrode.

3. Connect the other end of the cable to the rear of Alcolyzer Plus Beer (see

Chapter 6.2).

4. Insert the pH electrode into the protective tube carefully until the end of the

electrode touches the bottom of the pH measuring cell.

5. Tighten the protective tube by turning it clockwise.

The pH electrode is fixed.


17/129


C27IB02 17

Fig. 5 - 3 pH electrode Hamilton

6. Check that both Filling nozzle Luer Alcolyzer Plus are screwed into the

openings of the pH measuring cell (see Fig. 5 - 1).

5.2.2 Preparing and Mounting the pH Electrode "Orion

model 91-63"

Preparing

1. Remove the protective shipping cap from the sensing element and save for

storage.

2. Clean any salt deposits from the exterior by rinsing with distilled water.

3. Uncover the filling hole and add filling solution to the electrode.

4. Place the pH electrode in the electrode holder and suspend in air for 15

minutes to thoroughly wet the reference junction.

5. Shake the electrode (like a clinical thermometer) to remove air bubbles.

6. Soak the electrode in pH electrode storage solution for one hour.

Information:

To maintain an adequate flow rate, the level of filling solution must always

be above the reference junction.

The filling hole must be open whenever the electrode is in use.

Caution:

Once the junction is wet, do not allow the electrode to dry out.


18/129


18 C27IB02

Mounting

1. Screw the connecting cable (Mat. No. 62521) to the rear end of the pH

electrode.

2. Connect the other end of the cable to the rear of Alcolyzer Plus Beer (see

Chapter 6.2).

3. Insert the pH electrode into the pH measuring cell carefully until the end of

the electrode touches the bottom of the pH measuring cell.

Fig. 5 - 4 pH electrode Orion

4. Tighten the knurled nut (see Fig. 5 - 4). The pH electrode is fixed.

5. Check that both Filling nozzle Luer Alcolyzer Plus are screwed into the

openings of the pH measuring cell (see Fig. 5 - 1).

Knurled nut


19/129


C27IB02 19

5.3 Mounting the Hoses without Option pH/Color

Fig. 5 - 5 Hose connections without option pH/color

1. Cut a piece of the silicone hose 3 x 5 mm (1) approx. 24 cm in length.

2. Connect one end of the hose to the air pump outlet of the DMA.

3. Connect the Adapter Luer cone to the other end of the hose.

4. Connect a Tygon hose (2) approx. 26 cm in length to the upper end of the

SP-1m needle and to the front adapter of the density meter.

5. Connect a Tygon hose (3) approx. 22 cm in length to the rear adapter of the

density meter and to the lower adapter of Alcolyzer Plus Beer.

6. Connect a Tygon hose (4) approx. 22 cm in length to the upper adapter of


7. Connect the tube, Mat. No. 67982, (5) to the other end of the hose.

8. Connect a silicone hose 2x4 mm (6) to the tube.

9. Lead the silicone hose via the peristaltic pump of SP-1m to a waste vessel.

10. The length of this hose can be selected according to the distance of the

waste vessel.

4 (T)

3 (T)

2 (T)

5 (Tube)

6 (S) to waste

6

1 (S)

(T) .... Tygon

(S) .... Silicone


20/129


20 C27IB02

5.4 Mounting the Hoses with Option pH/Color

Fig. 5 - 6 Hose connections with option pH/color

1. Connect an adapter Luer cone (1) to each opening of the pH measuring cell.

2. Cut a piece of the silicone hose 3 x 5 mm (2) approx. 24 cm in length.

3. Connect one end of the hose to the air pump outlet of the DMA.

4. Connect the adapter Luer cone to the other end of the hose.

5. Connect a Tygon hose (3) approx. 26 cm in length to the upper end of the

SP-1m needle and to the front adapter of the density meter.

6. Connect a Tygon hose (4) approx. 22 cm in length to the rear adapter of the

density meter and to the lower adapter of Alcolyzer Plus Beer.

7. Connect a Tygon hose (5) approx. 22 cm in length to the upper adapter of


8. Connect the tube, Mat. No. 67982, (6) to the other end of the hose.

9. Connect a silicone hose 2x4 mm (7) approx. 50 cm in length to the tube.

10. Lead the silicone hose via the peristaltic pump of SP-1m to the front adapterof the pH measuring cell.

5 (T)

8 (S) to waste

3 (T)

6 Tube

7 (S)

4 (T)

2 (S)

1

(T) .... Tygon

(S) .... Silicone

to airpump


21/129


C27IB02 21

11. Connect a 2x4 mm silicone hose (8) to the rear adapter of the pH measuring

cell.

12. Lead this hose to a waste vessel.

13. Select the length of this hose according to the distance of the waste vessel.

5.5 Completing the Installation

1. Turn on the SP-1m and the density meter and wait for the initialization of

both instruments to be completed.

2. Configure the DMA 4500/5000 according toAppendix F.

3. Turn on Alcolyzer Plus Beer using the power switch at the rear of the

instrument.

4. Select the menu instrument settings > device listand configure the device

list as follows (for details about operating the menu, see Chapter 15.1):

Caution:

Before switching on Alcolyzer Plus Beer, check that the given voltage and

frequency (AC 100 to 240 V, 50 to 60 Hz) corresponds with the local

mains voltage and mains frequency. If large voltage fluctuations are to be

expected, the use of a constant voltage source (UPS) is recommended.

The non-fused earth conductor of the power cord must be connected to

earth.

Information:

Once switched on, Alcolyzer Plus Beer performs a system check and anattemperating procedure:

After transport, full stabilization of Alcolyzer Plus Beer might take up to

one day.

Usually Alcolyzer Plus Beer is ready for measurement after a start-up

period of one hour.

To guarantee an optimal temperature stability over a long time, always

leave the system switched on.


22/129


22 C27IB02

Fig. 5 - 7 Menu: instrument settings/device list

Now Alcolyzer Plus Beer initializes the communication between all

connected peripherals.

The COM2 interface of Alcolyzer Plus Beer is automatically configured

(connected to: PIB, baud rate: 9600, format: 8 bit No) and cannot be

changed unless densityis set to not used.

If all devices are detected properly, the following message is displayed:

Fig. 5 - 8 Device check

5. If for a certain peripheral the message "offline" is displayed, check the

interface settings and/or connection of the corresponding connection cable

(seeAppendix F).

INSTRUMENT SETTINGS

filling devicedensityCO2O2

SP-1m

device list

::::

DMA xxxx series

not usednot used

DEVICE CHECK

filling unitdensity :: SP-1mpassed


23/129


C27IB02 23

Fig. 5 - 9 Device check failed

To repeat the device check, select device check.

To edit the device list, select change device list.

6. Set the control parameters for SP-1m in the menu auto sampler settings

(see Chapter 15.2.9).

To make sure that the measured sample is fully replaced with the next

sample, we recommend using the control parameter 1100 (100 seconds

filling time, approx. 30 mL sample). For further information, see the SP-1m

instruction manual.

Fig. 5 - 10 Measuring system: Alcolyzer Plus Beer Analyzing System

BUS ERROR

filling unit : offline

device check 20 secchange device list


24/129


24 C27IB02

5.6 Connecting a Printer or PC

5.6.1 Connecting Alcolyzer Plus Beer to a Printer

Connect the printer cable (Mat. No. 64433) to the COM1 on the rear side of


Depending on the printer that you use, you can choose between 4 different printer

types for optimal communication between the connected printer and Alcolyzer

Plus.

Set the COM1 interface to the appropriate printer in the menu instrument

settings > COM1.

5.6.2 Connecting Alcolyzer Plus Beer to a PC

To transfer the measured results to a PC, connect the PC to Alcolyzer Plus Beer.

For further details, seeAppendix F.

1. Connect the interface cable DMA-PC (Mat. No. 72979) to the PC and to

COM1 on the rear side of Alcolyzer Plus Beer.

2. If you use the Anton Paar software VisioLab for Alcolyzer, set the COM1

interface to VisioLabin the menu instrument settings > COM1.

3. If you use the Anton Paar software AP-SoftPrint, set the COM1 interface to

AP-SoftPrintin the menu instrument settings > COM1.

Printer type Holding time

after 1 line

End-of-line

character

Remarks

printer_1 800 msec LF CR after 40

characters

printer_2 1500 msec LF

printer_3 800 msec LF

printer_4 10 msec LF+CR

AP-SoftPrint 10 msec LF


25/129

6 Functional Components

C27IB02 25


6.1 Display and Keypad

After switching on, Alcolyzer Plus Beer performs a self-test and the initialization.

6.1.1 Display in the Measuring Mode

Fig. 6 - 1 Display after the end of the measurement

A ... MethodThe selected method is displayed.

B ... Measuring status of the alcohol measurementFor details, see the reference manual, Appendix B.1.

C ... Time

D ... Sample nameE ... Measuring status of the color measurement

For details, see the reference manual, Appendix B.2.

F ... Measuring status of the pH measurementFor details, see the reference manual, Appendix B.3.

G ... Measuring status of the density measurementThe current status of the density measurement is displayed.

H ... Display area for measured values

I ... Information about the sample changer

J ... Soft keys

Information:

It may be necessary to adjust the contrast of the display with the black knob

"Display contrast" at the rear of the instrument.

BEERvalid09:44:21sample #validvalidvalid

SP-1m

%V/V 20 CBeer3c 7.4 EBCpH 4.43

1.00521 g/cm3

: stopped

A

B

C

D

E

F

G

H

I

J


26/129


26 C27IB02

Display of measured values:

The alcohol content is displayed in %V/V (2 trailing digits) at 20C (percent

volume per volume at the indicated temperature). If the measurement is not

finished, "....." is displayed. As long as the measurement is still in progress

("predetermined"), only one trailing digit is displayed. If the measuring resultis lower than -0.50%V/V or higher than 30.00%V/V, "low" or "high" is

displayed.

The color measurement is displayed in EBC or ASBC with one trailing digit

at 20C. If the measurement is not finished, "....." is displayed. As long as the

measurement is still in progress ("predetermined"), no trailing digit is

displayed. If the measuring result is lower than 0.5 EBC (-0.25 ASBC) or

higher than 130.0 EBC (66 ASBC), "low" or "high" is displayed.

The pH value is displayed in pH units (2 trailing digits) at 25C. If the

measurement is not finished, "....." is displayed.

Fig. 6 - 2 Display of further parameters

K ... Display area for calculated valuesSelectable, calculated parameters are displayed in this area after pressing

the key (see Chapter 7.5).

BEERvalid09:44:21sample #validvalidalcoholpErEaRDF

SP-1m

%V/V 20 CBeer3c 7.4 EBCpH 4.43 3.86 %m/m

11.12 %m/m 3.59 %m/m 1.80 %m/m 68.95 %

: stopped

KK


27/129


C27IB02 27

6.1.2 Soft Keys in the Measuring Mode

Toggle key:

To toggle between displays of measured andcalculated values

To enter the menu (the key is

available)

Selects the measuring method.

Selects the main menu for various settings and

configurations. Access to the main menu can be

protected by a password.

Starts printing.

This soft key is only displayed when interface

COM1 or COM2 is configured for a printer in the

menu instrument settings.

Switches the air pump on.

Switches the air pump off.

Starts an automatic measuring process.

If a sample changer SP-1m is connected to

Alcolyzer Plus Beer and activated, the key

on Alcolyzer Plus Beer is not displayed. Start the

measurement with the key on the sample

changer.

Starts the next measurement.

To enter the sample identification.

Selects the list for the sample identification.

Confirms the changes.

Enters the SOP menu.


28/129


28 C27IB02

6.2 Rear View

Fig. 6 - 3 Rear view

A ... Cable of the pH electrode

B ... pH electrode

C ... "air pump inlet": Connection for a desiccator

D ... "pH / color Module": Connection for the pH electrode

E ... "COM2": Serial interface RS 232 for connecting a density meter or a

printer

F ... "Keyboard" Connection for a keyboard and/or bar code reader

G ... Power inlet

H ... Power switch

I ... "COM1": Serial interface RS 232 for connecting a computer or printer

J ... "S-Bus": Connection for a sample changer

K ... "Display contrast"L ... Display contrast setting

M ... Cooling fins

A

B

C D E F G

I

J

KL

H


29/129

7 General Instrument Settings

C27IB02 29


7.1 Setting the Display Contrast

If necessary, adjust the display contrast with the "Display contrast" knob at the

rear of the instrument (see Chapter 6.2).

7.2 Setting Date and Time

Set date and time in the menu instrument settings > timeor dateusing the soft

keys , or . Press to confirm the settings.

7.3 Configuring the Measuring System

1. Press and to enter the main menu.

2. Select the menu instruments settings using the soft keys and .

Confirm with .

Fig. 7 - 4 Menu: instrument settings

7.3.1 Setting the General Parameters

Select the menu configurationto set general instrument settings.

Use the soft keys , and the key to select the parameter.

Press to confirm the settings.

Information:

For a detailed description of all setting options, see Chapter 15.2.2.

INSTRUMENT SETTINGS

device listCOM1COM2timedate

configuration


30/129


30 C27IB02

7.3.2 Defining the Peripheral Devices

Change to the menu instrument settings > device list to select the

connected peripherals like density meter, sample changer SP-1m or filling

device SF-1.

7.3.3 Defining the Interface Settings

Configure the two interfaces COM1or COM2for computer or printer in the

menu instrument settings.

7.4 Setting a Custom Function

You can define customized ethanol tables and color units.

7.4.1 Setting a User-Defined Ethanol Table (in %V/V)

If you want to use a different density/ethanol table than the OIML %V/V table, you

can edit a polynomial function (4th order) that calculates an ethanol value based

on your specific density/ethanol table.

Calculating the coefficients

The input value for the polynomial function is the ethanol %V/V value (c)

measured by Alcolyzer Plus Beer. The result of the calculation depends on the

entered coefficients according to the following formula.

Polynomial formula: Output (%V/V) = A0 + A1 x (c [%V/V]) + A2 x (c [%V/V]) 2+

A3 x (c [%V/V])3+ A4 x (c [%V/V])4

The default values are the polynomial coefficients valid for the OIML %V/V table(see Fig. 7 - 5).

Caution:

You can only use density/ethanol tables based on %V/V.
http://-/?-http://-/?-


31/129


C27IB02 31

Fig. 7 - 5 Default setting of the polynomial coefficients

1. To calculate the coefficients, take the appropriate table (%V/V) that converts

the measured density into a corresponding ethanol value.

2. By using an appropriate mathematical program, you can calculate the

polynomial coefficients which are based on the table data.

Editing the user-defined ethanol table

1. Select the menu user functions > user defined ethanol table.

2. Enter the 5 polynomial coefficients (A0 to A4) as calculated.

Activating the user-defined ethanol table

1. At Alcolyzer Plus Beer, select the menu instrument settings >

configuration > calc. parameter.

2. Select CU V/Vand press the key.

7.4.2 Setting a User-Defined Color Unit

If the color measurement according to EBC or ASBC is not desired, you can

define a user-defined color unit. This is done by correcting the EBC value c [EBC]

according to the formula shown in Fig. 7 - 6.

Editing the user-defined color unit

1. To enter the correction factor for the slope of the color measurement and the

offset, select the menu user functions > user defined color unit.

USER FUNCTIONS

user defined ethanol table

A0 : 9.982000e-01A1 : - 1.520000e-03A2 : + 1.924620e-05A3 : - 1.461290e-07A4 : - 4.574060e-09

+

Caution:

To obtain a correct alcohol adjustment of Alcolyzer Plus Beer, use the same

ethanol table in the DMA that you used for calculating the polynomial

coefficients (seeAppendix F).
http://-/?-http://-/?-


32/129


32 C27IB02

The following window is displayed:

Fig. 7 - 6 Menu: User functions/user defined color unit

2. Enter a slope value between 0 and 7.

Use the key to move the cursor. Edit the highlighted number using the and keys. Press to confirm the changes.

3. Enter an offset value between -10 and +10.

If not allowed values are entered, the slope is automatically set to 1.0 and

the offset to 00.0 after pressing .

Activating the user-defined color unit

1. Select the menu instrument settings > configuration > color unit.

2. Select CUand press the key.

7.5 Selecting the Output Data

When you select the parameters for display, printer and memory, you get a list

containing all parameters that are available for the predefined methods BEER,

CIDER, SOFT DRINK, WASH and MOLASSES (seeAppendix B.1).

If you then select your appropriate measuring method, you get only the

parameters that are of importance for this very method.

Example: If you select the parameter p (original extract) [%Plato], this parameter

is only available in the methods BEER, WASH and MOLASSES.

USER FUNCTIONS

user defined color unit

c [CU] = .000 * c [EBC] + 00.001


33/129


C27IB02 33

7.5.1 Selecting the Output Data for the Display

Change to the menu utilities > display configuration.

Select up to 5 parameters using the soft keys , and thekey.


7.5.2 Selecting the Output Data for the Printer

You can select output data for the printer and save this selection in a printer

protocol. A printer protocol consists of a head and parameters. There are 4

factory-set printer protocols and 6 free printer protocols available.

Factory-set printer protocols:

- Printer protocol No. 6, used with BEER method

- Printer protocol No. 7, used with CIDER method

- Printer protocol No. 8, used with WASH and MOLASSES method

- Printer protocol No. 9, used with SOFT DRINK method.

For details of the settings, seeAppendix B.2.

If required, you can change these pre-settings according to your demands.

To define or change a printer protocol, change to the menu utilities > output

configuration > printer protocol:

To activate/deactivate the parameters, use the key.


7.5.3 Selecting the Output Data for the Data Memory

Change to the menu utilities > output configuration > data memory.

You can activate up to 10 parameters to be stored in the data memory:

To activate/deactivate the parameters, use the key.


7.6 Settings for the Sample Changer

Enter the control parameter for SP-1m in the menu auto sampler settings

as described in the SP-1m instruction manual.

Information:

If data should be sent to a LIMS system, activate additionally the parameters

alcohol cond.and color cond.


34/129

8 Setting the Measuring Methods

34 C27IB02

This menu is only displayed if a sample changer is connected and configured

in the menu instrument settings > device list > filling device.


8.1 Description of the Measuring Methods

Alcolyzer Plus Beer provides 10 different methods:

Fig. 8 - 1 Menu: method settings

Method 1: BEER

Standard method for beers. This method can be used for almost all samples.

Method 2: CIDER

Method for ciders.

Method 3: SOFT DRINK

Method for alcohol-free beverages.

Method 4: WASH

Method for wash. Wash is an intermediate product of whisky production. It is an

alcohol containing liquid produced in the fermentation of worts by yeasts. The

alcohol content in wash varies between 7 and 10%vol.

Information:

We recommend setting the following filling times: Without option pH/color: 80 seconds (sample changer parameter "1080").

With option pH/color: 100 seconds (sample changer parameter "1100").

METHOD SETTINGS

no. 1no. 2no. 3no. 4no. 5no. 6no. 7no. 8

no. 9no. 10

::::::::

::

BEERCIDERSOFT DRINKWASH

new method

MOLASSES


35/129


C27IB02 35

Method 5: MOLASSES

Method for molasses. Molasses is a honey-like, dark-brown sugar syrup which is

used as a fermentation raw material in the production of bioethanol. With this

method, you can determine the ethanol content of fermenting or ready-fermented

molasses.

Methods 6 to 10:

These methods are sample-specific and can be edited as described in Chapter

8.2.

8.2 Editing a Measuring Method

8.2.1 Editing a Predefined Method

You can edit the predefined methods "BEER", "CIDER", "SOFT DRINK", "WASH"

or "MOLASSES" to assign another printer protocol or enter a residual CO2content.

1. In the menu method settings, select one of the predefined methods and

confirm with .

2. In protocol no.:, you can select one of 10 printer protocols to assign it to the

selected method (For editing a printer protocol, see Chapter 7.5.2).

8.2.2 Editing a New Method

You can edit a new method to assign a special printer protocol (see Chapter 7.5.2)

or to enter a sample-specific alcohol correction factor (see Chapter 12.4). For the

types "BEER", "CIDER" and "SOFT DRINK", the residual CO2can be entered

(see Chapter 8.2.1).

1. Select a row between 6 and 10 in the menu method settings.

"new method" is displayed.

2. Confirm with .

3. Enter a name for the method and confirm the method name with .

Information:

If you measure fermenting molasses, we recommend to filtrate the sample

prior to measurement.

Information:The menu item residual co2 is only used with PBA-B and the methods

"BEER", "CIDER" and "SOFT DRINK".


36/129


36 C27IB02

4. Select a type"BEER", "CIDER", "SOFT DRINK", "WASH" or "MOLASSES"

for the new method.

The type has to correspond with the sample to be measured.

5. If necessary, enter an offset between 0.99%vol and +0.99%vol (seeChapter 12.4).

6. If necessary, enter a pH-offset between -0.99 and +0.99 in pH-deg. offset.

7. In protocol no.:,you can assign a printer protocol from 1 to 10 to the new

edited method.

For details about editing a printer protocol, see Chapter 7.5.2.

8. Press to confirm the settings.

Fig. 8 - 2 Menu: method settings/new method/name

EDIT METHOD no. 6

typeoffsetpH-deg.offset

BEER:::

+0.50 %V/V

name special beer:

protocol no.residual co2

::

+0.50050.00 g/l

Information:

The menu item residual co2 is only used with PBA-B and the methods

"BEER", "CIDER" and "SOFT DRINK".


37/129


C27IB02 37

8.3 Selecting the Measuring Method

1. Press the key in the measuring window.

The following window is displayed:

Fig. 8 - 3 Selecting a method

2. Use the key to toggle between the different methods.

3. Select the appropriate method (e.g. "BEER") with the key.

The selected method is displayed in the left upper corner.

BEER

BEERvalid09:44:21

validvalid

method :

%V/V 20 C

c 7.4 EBCpH 4.43

Information:

If you have assigned another method to a certain sample in the sample list,

this method instead of the selected method is used to measure the sample

(see Chapter 12.1.3.1).


38/129

9 SOP (Standard Operating Procedure)

38 C27IB02


To ensure that Alcolyzer Plus Beer gives correct results, check the adjustments

of the measured parameters regularly.

The integrated SOP (Standard Operating Procedure) allows you to perform an

automated control measurement of all measuring parameters as well as an

adjustment afterwards.

Carrying out the SOP

1. Select the menu utilities > SOP configuration and choose SOP #1 or

SOP #2.

"SOP #1" is defined as the "routine" SOP and contains the parameters water,

alcohol, pH1 and pH2.

2. Place the appropriate control solutions in the correct order into the sample

changer magazine (for preparing the control solutions, see Chapter 10).

The following control solutions are used for the SOP:

- Bi-distilled water to check the zero point of the alcohol and the color

adjustment

- A binary ethanol/water solution with an alcohol content of 8 to 12%V/V to

check the "known concentration" of the alcohol adjustment

- pH buffer pH 7.00 and

- pH buffer pH 4.01 to check the pH adjustment

3. Press the key in the measuring window of Alcolyzer Plus Beer to

access the following menu:

Information:

The SOP can only be used in combination with a sample changer.

Information:

Carry out the "routine" SOP once a day. If desired, an additional SOP

(SOP #2) can be defined as an "extended" SOP with additional parameters

(e.g. color standard).


39/129


C27IB02 39

Fig. 9 - 1

Use the key to toggle between "SOP #1" and "SOP #2".

4. Press the key to start the SOP.

5. Press the key on the sample changer.

The control solutions are measured automatically.

After the last measurement, the following window is displayed:

Fig. 9 - 2

6. If a measured value deviates significantly from the desired value, this value

can be readjusted now.

STANDARD OPERATING PROCEDURE #1

#1:2:

3:4:

#

##

wateralcohol

pH 1pH 2

adjust ?adjust ?

adjust ?


water

alcoholpH 1pH 2

SP-1m :

-0.01 %V/V 0.0710.01

EBC %V/V

4.02 pH 6.99 pH

stopped

adjust ?

10: 32: 54


40/129


40 C27IB02

7. Highlight the respective row "adjust?" and press the key (see Fig. 9 - 2).

8. Enter the desired value and press .

Fig. 9 - 3

The following window is displayed (in this example, the alcohol value has

been readjusted):

Fig. 9 - 4

Information:

The adjustment of the known concentration for the alcohol can only be

performed successfully if an accurate density meter and an appropriate

density/alcohol concentration table are available.

If the known concentration for the alcohol shall be adjusted, the alcohol

value measured by the DMA is displayed in a line below the SOP

parameters after pressing the key. This value can be taken directlyfor the readjustment.


water

alcoholpH 1pH 2

adjust ?adjust ?

adjust ?

10: 34: 27

-0.01 %V/V 0.0710.01

EBC %V/V

4.02 pH 6.99 pH

1concentration: 0.05 %V/V

adjust ?adjustedadjust ?


water

alcoholpH 1pH 2

-0.01 %V/V 0.0710.05

EBC %V/V

4.02 pH 6.99 pH

adjust ?

10: 35: 16


41/129

10 Checking the Adjustment Validity

C27IB02 41

9. Finish the SOP by pressing the key or the key when all

parameters are readjusted.


If no SOP (see Chapter 9) is carried out, check the adjustments regularly as

described below.

10.1 Checking Procedure without Option pH/Color

Before each series of measurements, check the zero point using degassed,

bi-distilled water.

To check the validity of the slope of the adjustment, use a water/ethanol

solution of known concentration. Perform a slope check at least once everyweek.

10.1.1 Performing a Checking Procedure with Water (0%V/V)

1. Boil fresh, bi-distilled water for several minutes to remove dissolved gas.

2. Fill a clean glass flask full with the boiled water and cover it.

3. Wait until the water has cooled down to 20 2C.

4. Clean the measuring cell as described in Chapter 14.

Information:

If one (or both) pH buffer deviates too much from the correct value, the

measured pH value and the measured temperature will be displayed in a

line below the SOP parameters after confirming the question "adjust ?".

The pH value can then be corrected according to the pH/temperature

table of the corresponding buffer.

The readjustment for the pH measurement is carried out for both

adjustment points simultaneously. Therefore, only one "adjust? is

displayed for both buffers.

Information:

For the color adjustment, it is only necessary to adjust the zero point with

bi-distilled water regularly. If it is desired to check also the known concentration for the color

measurement (e.g. with a standard beer or color standard) this parameter

can be added to the SOP. If the known concentration for the color

measurement shall be adjusted, the color value of the color standard can

be entered in a line below the SOP parameters after pressing the key. Color standards are available at Anton Paar. Contact your local distributor.


42/129


42 C27IB02

5. Fill a sample vial with degassed, bi-distilled water and put it into the

magazine of the sample changer.


7. Wait until the measuring status displays "valid".

8. If the displayed value is 0 0.03%V/V and is stable for a few minutes,

measurements can be performed.

9. If the displayed value is outside this concentration range, clean the

measuring cell and perform another control measurement.

10. If the measuring result is still out of range (0 0.03%V/V) perform a zero

point adjustment (see Chapter 11.1.1).

11. If "no sample" is displayed, clean the measuring cells according to Chapter

14.1.3or Chapter 14.2.4(with option pH/color).

12. Then perform an adjustment for the zero point and for the known

concentration.

Checking the validity of the density adjustment

1. As soon as the DMA gives a valid result (flashing display), check the density

reading.

2. If the result deviates more than 0.0001 g/cm3(DMA 4500) or 0.00005 g/cm3

(DMA 5000) from the reference value (0.998203 g/cm3), perform a cleaning

procedure according to Chapter 14.

3. Repeat the checking procedure.

4. If the density result still exceeds the max. allowed deviation, perform an air/

water adjustment for the density meter (see Chapter 11.3).

Information:

If a density meter (DMA 4500, DMA 5000) is connected to Alcolyzer Plus

Beer, check also the validity of the density adjustment as described below.


43/129


C27IB02 43

10.1.2 Performing a Checking Procedure with a Water/

Ethanol Solution of Known Concentration

1. Prepare a water/ethanol solution with a concentration between 8 and

12%V/V.

2. Cover the flask and leave the solution for several hours at a temperature of

20 3C.

Before filling the measuring cell, the sample must be free of air bubbles.

3. Clean the measuring cell, if necessary (see Chapter 14).

4. Fill a sample vial with the water/ethanol solution and put it into the magazine

of the sample changer.


6. Wait until Alcolyzer Plus Beer and the DMA show "valid".

7. If the difference between the measuring values from Alcolyzer Plus Beer and

the DMA is less than 0.03%V/V and the results remain stable within this

range for a few minutes, the adjustment of Alcolyzer Plus Beer is correct.

8. If the displayed value is out of this range, clean the measuring cell andperform another control measurement.

9. If the measuring result is still out of range, perform an adjustment using a

water/ethanol solution (see Chapter 11.1.2).

Information:

This measurement can only be performed successfully if an accurate density

meter and an appropriate density / alcohol concentration table is available.

Caution:

Only use high-proof ethanol without denaturant!

Information:

If Alcolyzer Plus Beer is used in combination with a DMA 4500/5000, the

alcohol concentration is directly displayed on the density meter.


44/129


44 C27IB02

10.2 Checking Procedure with Option pH/Color

10.2.1 Checking Procedure for the Measuring Parameter

"alcohol"

The checking procedure for the measuring parameter "alcohol" is performed as

described in Chapter 10.1.

10.2.2 Checking Procedure for the Measuring Parameter "pH"

Check the validity of the pH adjustment with buffers pH 7 and pH 4 once a day

(before starting the routine measurements).

Control measurement for buffer pH 7

1. Fill a sample vial with buffer pH 7 and place it in the sample changer

magazine.


After filling is completed, the measurement starts automatically.

3. Wait until the pH value is displayed in the measuring window ("valid").

This value is valid for 25C.

4. If the difference between the measured pH value and the set point is less

than 0.05 pH units, the pH adjustment is correct.

5. If the displayed value is out of this range, fill the pH measuring cell with the

same buffer and repeat the control measurement.

Caution:

Always use fresh pH buffers.

Information:

Instead of using the sample changer, one of the following filling procedures

can be used:

Disconnect the silicone hose that is plugged to the front adapter of the pH

measuring cell. Fill a 20 mL syringe with the buffer. Connect the syringe to

the front adapter of the pH measuring cell and fill in the buffer.

Or:

Remove the pH electrode from the pH measuring cell and immerse the

electrode into a vessel filled with pH buffer. Ensure that the buffer

temperature does not deviate significantly from the temperature in the pH

measuring cell as the temperature sensor for the pH measurement is

located in the pH measuring cell.


45/129

11 Adjusting the Instrument

C27IB02 45

6. If the displayed value is still out of range, perform an adjustment (see

Chapter 11.2.3).

Control measurement for buffer pH 4

The control measurement is performed in the same way as for buffer pH 7.

10.2.3 Checking Procedure for the Measuring Parameter

"color"

Checking procedure with water (0.0 EBC)

This procedure should preferably be performed together with the checking

procedure for 0% alcohol (see Chapter 10.1.1)

1. If the displayed value is 0 0.3 EBC (0 0.15 ASBC), measurements can beperformed.

2. If the displayed value is out of this range, clean the measuring cells and

repeat the control measurement.

3. If the measuring result is still out of range, perform a "zero" adjustment (seeChapter 11.2.2).


11.1 Adjustment without Option pH/Color

Regular adjustments are performed with degassed bi-distilled water (0%V/V

alcohol, zero point).

Extended adjustments are performed with degassed bi-distilled water and a

water/ethanol solution of exactly known concentration in the range of 2 to

30 %V/V, preferably 8 to 12%V/V.

An extended adjustment is necessary if the checking procedure with a water/

ethanol solution of known concentration shows deviations of more than

0.03 %V/V from the reference value, even after correct zero point

adjustment.

Information:

During the first months, usually a drift of the color value of approx. 0.1 EBC

per day can be observed. This drift (in the positive direction) is a result of a

decreasing LED intensity.


46/129


46 C27IB02

If the adjustment of Alcolyzer Plus Beer is properly performed, it is sufficient

to check only the zero point before each series of measurements.

11.1.1 Adjustment with Water (Zero Point)

1. Boil fresh, bi-distilled water for several minutes to remove dissolved gas.

2. Fill a clean glass flask full with the boiled water and cover it.

3. Wait until the water has cooled down to approx. 20 2C.

4. Select Alcolyzer Plus Beer menu adjustment > adjust alcohol > zero.

5. Answer the question "Do you really want to adjust?" with .

If there is no bi-distilled water in the measuring system, press on

Alcolyzer Plus Beer. Then press the key on the sample changer.

The bi-distilled water is now filled into the measuring system by the sample

changer. After the filling procedure is completed, the adjustment procedure

starts automatically.

If bi-distilled water has already been filled into the measuring system, press

the key instead of the key to start the adjustment

procedure immediately.

6. Wait until the adjustment has been completed.

Information:

Before the adjustment, Alcolyzer Plus Beer has to be turned on for at leastone hour. However, after transport full stabilization of Alcolyzer Plus Beer

might take up to one day.

The ambient temperature should be stable to < 3C.

Caution:

Before starting the adjustment, ensure that the measuring cells are properly

cleaned (see Chapter 14).


47/129


C27IB02 47

Fig. 11 - 1 Successful adjustment

7. If the measurement value is stable to 0.02%V/V, the adjustment is

successful and "adjustment saved" is displayed (see Fig. 11 - 1).

Press to confirm the adjustment.

Press the key three times to return to the measuring window.

8. If "no stability - restart" is displayed after the adjustment, the required stability

of the measuring result could not be achieved. Problems during sample

filling (for example, air bubbles in the cell) could be the reason.

Clean the cells again and repeat the adjustment with bi-distilled water.

ADJUSTMENT ALCOHOL

S1 = 87.30 % of factory adjustment

adjustment 0.00 %V/V

adjustment saved

Information:

Additionally, the value S1 gives information about the measured signal

intensity of the current adjustment compared to the signal intensity at the

factory adjustment. This value decreases by-and-by due to ageing of the LED

and/or coating of the measuring cell.

If the S1 value is below 80%, clean the measuring cell thoroughly (see

Chapter 14) and repeat the zero adjustment. If the S1 value is still too low,

contact your Anton Paar representative.

Information:

After this adjustment we recommend performing the zero adjustment for the

optional color measurement because the bi-distilled water which is inside the

cells can be used for the zero adjustment of the color measurement.
http://-/?-http://-/?-


48/129


48 C27IB02

11.1.2 Adjustment with a Water/Ethanol Solution of Known

Alcohol Concentration

1. Prepare a water/ethanol solution.

2. Cover the flask and leave the solution for several hours at 20 3C. Before

filling the measuring cell, the sample must be free of gas bubbles.

3. Select the Alcolyzer Plus Beer menu adjustment > adjust alcohol > known

concentration.


5. If there is no water/ethanol solution in the measuring system, press

on Alcolyzer Plus Beer.


The water/ethanol solution is now filled into the measuring system by the

sample changer. After the filling procedure is completed, the adjustment

procedure starts automatically.

If a DMA 4500/5000 is connected and configured properly (seeAppendix F),

"DMA: measuring" is displayed on Alcolyzer Plus Beer. As soon as "DMA:

valid" is displayed, the alcohol concentration that comes from the DMA is

displayed in the first line of Alcolyzer Plus Beer display and can be used

directly as the "known concentration" value for the adjustment.

7. Press the key to continue the adjustment.

8. Wait until the adjustment is finished.

Caution:

Only use high-proof ethanol without denaturant.

Information:

The concentration of the water/ethanol solution for the adjustment must be

between 2 and 30%V/V, preferably between 8 and 12%V/V alcohol.

Caution:



Information:

If a water/ethanol solution has already been filled into the measuring system,

press the key instead of the key.


49/129


C27IB02 49

9. If the measurement is stable to 0.02%V/V, the adjustment was successful

and "adjustment saved" is displayed.



10. If "adjustment failed" is displayed after the adjustment, the required stability



Clean the cells again and repeat the adjustment with a water/ethanol

solution.

11.2 Adjustment with Option pH/Color

11.2.1 Adjustment Alcohol

The adjustment for the measuring parameter "alcohol" is performed as described

in Chapter 11.1.

11.2.2 Adjustment Color

For the color adjustment, use the delivered color adjustment set.

11.2.2.1 Adjustment with Water (Zero)

1. Fill a clean sample vial with the delivered water standard (ultra pure water)

and place the vial in the sample magazine.

2. Select the menu adjustment > adjustment color > zero.


4. Press the key on Alcolyzer Plus Beer.


The ultra pure water is filled into the measuring system.

Information:

Alcohol solutions have a tendency to form gas bubbles. Handle the sample

with care.

Caution:




50/129


50 C27IB02

After the filling procedure is completed, the adjustment procedure starts

automatically.


Fig. 11 - 2 Successful adjustment

If the measurement value is stable, the adjustment is successful,

"adjustment saved" is displayed (see Fig. 11 - 2).



7. If "no stability - restart" is displayed after the adjustment, the required stability



Clean the cells again and repeat the adjustment with bi-distilled water.

ADJUSTMENT COLOR

S = 98.30 % of factory adjustment

adjustment 0.00 EBC adjustment saved

Information:

Additionally, the value S gives information about the measured signal intensity

of the current adjustment compared to the signal intensity at the factory

adjustment. This value decreases by-and-by due to ageing of the LED and/orcoating of the measuring cell.

If the S value is below 80%, clean the measuring cell thoroughly (see Chapter

14) and repeat the zero adjustment. If the S value is still too low, contact your

Anton Paar representative.

Information:

After the color adjustment has been done properly, it is only necessary to

check the zero point adjustment with bi-distilled water regularly.
http://-/?-http://-/?-


51/129


C27IB02 51

11.2.2.2 Adjustment with a Color Standard (Tartrazine) of Known

Concentration

1. Fill a sample vial with the delivered color standard (tartrazine) and place it in

the sample changer magazine.

2. Select the menu adjustment > adjust color > known concentration.


4. Press the key on Alcolyzer Plus Beer.


The standard is filled into the measuring cells.

6. Enter the EBC value of the delivered standard in the line "color".

7. Press the key to continue with the adjustment.


9. If the measurement is stable, the adjustment was successful and

"adjustment saved" is displayed.



10. If "adjustment failed" is displayed after the adjustment, the required stability



Clean the cells again and repeat the adjustment with the color standard.

Information:

The concentration of the color standard for the adjustment must be between1.00 and 60.00 EBC (0.50 to 30.48 ASBC), preferably at approx. 50.00 EBC

(25.40 ASBC).

Information:

After the color adjustment has been done properly, it is only necessary to

check the zero point adjustment with bi-distilled water regularly.


52/129


52 C27IB02

11.2.3 Adjustment pH

The adjustment of the pH electrode is performed with two buffers (pH 7 and pH 4).

Check the validity of the pH adjustment with buffers pH 7 and pH 4 once a day

(before starting the routine measurements). For details, see Chapters 9 and

10.2.2.

For the pH buffers "Hamilton Duracal" the buffer data (pH value depending on the

temperature) are integrated in Alcolyzer Plus Beer.

If the pH electrode is adjusted with these buffers, the correct buffer data are

selected automatically.

If you use a different buffer to "Hamilton Duracal", the buffer data (pH value

depending on the temperature) can be entered in the menu edit buffer data. If

the pH electrode is then adjusted with these buffers, the correct buffer data areselected automatically.

Caution:

Always use fresh pH buffers.

Temperature (C) Buffer pH 7.00 Buffer pH 4.01

5 7.09 4.01

10 7.06 4.00

15 7.04 4.00

18 7.03 4.00

20 7.02 4.00

22 7.01 4.00

25 7.00 4.01

30 6.99 4.01

35 6.98 4.02

40 6.97 4.03

45 6.97 4.04

50 6.97 4.05

Information:

For the adjustment, the first buffer should be pH 7, the second buffer should

be pH 4.


53/129


C27IB02 53

11.2.3.1 Automatic

The adjustment is usually performed with "Hamilton Duracal" buffers. However,

the adjustment can be performed with other buffers. The data of these buffers

have to be entered in the menu edit buffer data. The edited buffers have to be

selected in the menu sort buffer data.

1. Select menu adjustment > adjust pH > automatic.

"clean pH sensor/expose to buffer 1" is displayed.

2. To fill buffer pH 7 in the measuring cells using the sample changer SP-1m,

press first the key on the Alcolyzer and then the key on

the sample changer.

3. After the filling procedure is completed, wait until the voltage and the pH

value is stable (also check if the pH value is plausible) before pressing the

key to start the adjustment.

"Standard 7.00" (or the name of your buffer) as well as a number (in row"adjust") is displayed.

4. Press the key.

The adjustment with the first buffer is finished.


5. Fill buffer pH 4 in the measuring cells and wait until the voltage and the pH

value is stable (also check if the pH value is plausible).

6. Then press the key to start the adjustment.

Information:

Instead of using the sample changer, one of the following filling procedurescan be used:



the front adapter of the pH measuring cell and fill in the buffer. Use at least

15 mL buffer to make sure that the previous liquid in the pH measuring

cell is fully replaced.

Or:Remove the pH electrode from the pH measuring cell and immerse the

electrode into a vessel filled with pH buffer. Ensure that the buffer




Information:

If "no buffer saved" is displayed, the measured voltage deviates from the

stored tables (Hamilton or your own tables).


54/129


54 C27IB02

"Standard 4.01" (or the name of your buffer) is displayed. The adjustment is

finished. The voltages of the adjustment data (for the offset and the slope)

are displayed.

7. Press the key to finish the adjustment.

11.2.3.2 Manual

The pH adjustment can also be performed manually. To do this, any buffer can be

used.

1. Select menu adjustment > adjust pH > manual.


2. To fill buffer pH 7 in the measuring cells using the sample changer SP-1m,

press first the key on the Alcolyzer and then the key onthe sample changer.

3. After the filling procedure is completed, wait until the voltage and the pH

value is stable (also check if the pH value is plausible) before pressing the

key to start the adjustment.

4. Enter the theoretical pH value of the buffer (pH value depending on the

current temperature) using either the , , keys or a keyboard.

5. Then press .

6. Wait until the adjustment with the first buffer is finished.

7. Then press the key.


8. Fill buffer pH 4 in the measuring cells and wait until the voltage and the pH

value is stable (also check if the pH value is plausible).

9. Then press the key to start the adjustment.

Information:

Instead of using the sample changer, one of the following filling procedures

can be used:



the front adapter of the pH measuring cell and fill in the buffer. Use at least

15 mL buffer to make sure that the previous liquid in the pH measuring

cell is fully replaced.

Or:Remove the pH electrode from the pH measuring cell and immerse theelectrode into a vessel filled with pH buffer. Ensure that the buffer





55/129


C27IB02 55

10. Enter the theoretical pH value of the buffer (pH value depending on the

current temperature) using either the , , keys or a keyboard.

11. Then press .

12. Wait until the adjustment with the second buffer is finished.

The voltages of the adjustment data (offset and slope) are displayed.

13. Press the key to finish the adjustment.

11.2.3.3 Edit Buffer Data

If you use a different buffer to "Hamilton Duracal" for the adjustment, the buffer

data (pH value depending on the temperature) can be edited in this menu.

In the first two rows of the list the buffers "Hamilton Duracal" are predefined. Thedata of these buffers are already programmed and cannot be edited.

The rows 3 10 are individually editable. For any buffer the buffer data can be

entered and stored using a certain name for the new edited buffer.

Entering buffer data

1. Select a row between 3 and 10 using the keys and .

"new buffer" appears on the display.

2. Press to enter the designation (e.g. "buffer pH 4") of the new bufferusing the , , keys or a keyboard.

3. Press the key.

Now the list can be entered (temperature and corresponding pH value).

4. Use the key to enter the data. Use the key to move the cursor.Use the key to go to the next row. Press to save all entered data.

5. Answer the question "save new pH buffer?" with the key.

Deleting buffer data

1. To delete a newly edited buffer, press the key.

2. Answer the question "really clear buffer data?" with and press the

key.

Caution:At least two pairs of temperature and corresponding pH value that enclose the

temperature, at which the pH is later measured, have to be entered.


56/129


56 C27IB02

Alcolyzer Plus Beer

Documents

Transcript of Alcolyzer Plus Beer