Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600...

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Agilent Infinity II UV Agilent Infinity II Key words Authors

Transcript of Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600...

Page 1: Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600 nm 500 nm 600 nm 310 nm 500 nm 600 nm 310 nm sudan orange G patent blue VF sodium

Agilent Infinity II

UV

Agilent Infinity II

Key words

Authors

Page 2: Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600 nm 500 nm 600 nm 310 nm 500 nm 600 nm 310 nm sudan orange G patent blue VF sodium

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1260 Agilent Infinity II HPLC

1260 Infinity II Quaternary Pump (G7111B)

1260 Infinity II Multisampler (G7167A)

1260 Infinity II Diodearray Detector (G7115A)

1260 Infinity II Fraction Collector (G1364F)

OpenLAB CDS ChemStation C01.08

UV

UV

1.

Agilent Delay and Checkout Calibrant (P/N:5190-8223)

patent blue VF sodium,

sudan orange G sunset yellow FCF 2 mg/mL DMSO

Patent blue VF sodium 600 nm Sudan orange

500 nm Sunset yellow FCF 400 nm

310 nm

2. ①

3. ②

HPLC

移動相A 0.1%ギ酸

移動相B アセトニトリル

流速 5.0 mL/min

グラジエント 10%B (0min)→95%B(5min)

分析時間 5分(ポストラン2分)

カラムZORBAX SB-C18,9.4×150 mm, 5μm(P/N:728975-302)

カラム温度 室温

注入 1μL

検出 A: 600 nm, B: 500 nm, C: 400 nm, D 310 nm

Signal A 600 nm D 310 nm

ピーク検出モード

Threshold Threshold

Threshold 100mAU 100mAU

Signal A 600 nm B 500 nm

ピーク検出モード

Threshold Threshold

Threshold 100mAU 100mAU

Page 3: Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600 nm 500 nm 600 nm 310 nm 500 nm 600 nm 310 nm sudan orange G patent blue VF sodium

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1.

600 nm, 500 nm

310 nm

2.

3.

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A 600 nm 100 mAU

B 310 nm 100 mAU

4.

600 nm

500 nm

600 nm

310 nm

500 nm

600 nm

310 nm

sudanorange G

patent blue VF sodium

Page 4: Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600 nm 500 nm 600 nm 310 nm 500 nm 600 nm 310 nm sudan orange G patent blue VF sodium

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UV

4.

5.

Agilent Isocratic Standard P/N: 01080-68704

dimethyl phthalate 1.192

g/L, diethyl phthalate 1.189 g/L, biphenyl 0.0793 g/L, o-

terphenyl 0.238 g/L

5 2

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5.

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A B

6.

Agilent Infinity II

HPLC

移動相 0.1% ギ酸/ アセトニトリル = ( 45/55 , v/v )

流速 1.0 mL/min

分析時間 5 分

カラムZORBAX Eclipse Plus C18 4.6×50 mm, 5 μm( P/N : 959946-902 )

カラム温度 室温

注入 1μL

検出 A: 210 nm

HPLC

移動相A 0.1%ギ酸

移動相B アセトニトリル

流速 1.0 mL/min

グラジエント 30%B (0min)→90%B(5min)

分析時間 7分(ポストラン2分)

カラムZORBAX Eclipse Plus C18 4.6×50 mm, 5 μm

( P/N : 959946-902 )

カラム温度 室温

注入 1μL

検出 210 nm

Page 5: Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600 nm 500 nm 600 nm 310 nm 500 nm 600 nm 310 nm sudan orange G patent blue VF sodium

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0-3 6 3 7

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Signal 210 nm

ピーク検出モード Slope

Up Slope 20 mAU /s

Down Slope 5 mAU /s

Signal 210 nm

ピーク検出モード Slope

Up Slope 1 mAU /s

Down Slope 1 mAU /s

Signal 210 nm

ピーク検出モード Threshold and Slope

Threshold 1 mAU

Up Slope 50 mAU /s

Down Slope 5 mAU /s

Time/min 0-1.0 1.0-4.5 4.5-

ピーク検出モード OffThreshold

and Slope

Threshold

and Slope

Threshold - 1 mAU - 500 mAU

Up Slope - 50 mAU /s 50 mAU /s

Down Slope - 5 mAU /s 5 mAU /s

Page 6: Agilent Infinity II3 1 1. 600 nm, 500 nm 310 nm 2. 3. 3. 4 A 600 nm 100 mAU B 310 nm 100 mAU 4. 600 nm 500 nm 600 nm 310 nm 500 nm 600 nm 310 nm sudan orange G patent blue VF sodium

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Agilent Infinity II

www.agilent.com/chem/jp

0120-477-111

[email protected]

© Agilent Technologies, Inc. 2018

Printed in Japan, March 29, 2018

LC-201803ND-001