Activities on sweet cherry genetic

resources at CRA-FRF and DEMETRA

Daniela Giovannini

CRA-FRF, Unità di Ricerca per la

Frutticoltura di Forlì

Annalisa Marchese

Dipartimento DEMETRA

Università di Palermo

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

Italy displays many, heterogeneous climatic conditions

hundreds of local varieties

progressive loss of traditional varieties

links with the territory

source of useful traits for breeding

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

Sweet cherry in Italy has been cultivated for more than 2000 years

Initiatives, at the local level, to preserve heritage varieties

Corniola

Morphological & phenological characterization of the collected germplasm

Fiore

Duroncino di Cesena

Morphological & phenological characterization of the collected germplasm

Don Antoni

Cappuccia di Chiusa Sclafani

Morphological & phenological characterization of the collected germplasm

Kronio

Morphological & phenological characterization of the collected germplasm

Naturally self-compatible (Marchese et al., 2007)

S5’S6, where S5’ derives from a mutation in the S5 pollen component

Morphological & phenological characterization of the collected germplasm

Bombardune

Carrammendula 1

Zuccaredda

Caddusa Moscatella C. Sclafani

Cappuccia Castelbuono Nucigliera

Cappuccia Imperiale Niredda Cappuccia

Carrubbedda Nireddi

Cristallina Puntalazzese

Furticchiara Quarantana

Itala S.Mela Raffiuna S.Mela

Kronio

Maiolina S.Mela

Sant’Angelo S.Mela

San Pitrisa Zappia

Corniola A Durone di Cesena B

Corniola B Durone Nero I

Corniola C Durone Nero II

Durella A Fiore A

Durella B Fiore B

Durona di S.Giovanni Gemella

Duroncino di Cesena Morandina

Durone Compatto di Vignola Morena A

Durone di Cesena A Morena B

Accessions studied & their territory of origin

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

Abenavoli bianco Citra Napoletana

Abenavoli nero Cuore Aspromonte Niredda

Amarena grecanica Cuore Serre Piede corto

Bombardune Grammendula 1 San Pietro 1

Cannamele Grammendula 2 San Pietro 2

Cappucciarica 1 Iancuzza napoletana Santa Nutrice

Cappucciarica 2 Lombardune Semenzale durone

Carraffune Maiatica Semenzale morbido

Carraffune bianco Maiatica rossa Vallescura 1

Carraffune biondo Maiolina Piana Vallescura 2

Carraffune nero Maiolina Serre Zuccaredda

Carrammendula A Moddacchia 1 Zuccarigna

Carrammendula B Moddacchia2

(Tobutt & Clarke, 2009)

Standard set of primers

& Reference cvs.

Genetic diversity assessment

64 accessions

5 Reference cvs.

59 Italian accessions

16 from E.Romagna

14 from Sicilia

29 from Calabria

References

(source EMR)

Noble

Noir de Meched

Napoleon

Goodnestone Black

Colney

Primer Rank

CPPCT006 1

CPPCT22 2

EMPA017 3

EMPA002 4

EMPaS10 5

EMPaS12 6

EMPaS01 7

BPPCT037 8

PceGA34 9

EMPaS06 10

EMPaS02 11

UDP98-412 12

EMPaS14 13

EMPA026 14

PS05C03 15

EMPA003 16

10 out of 16 SSRs used

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

http://www.bordeaux.inra.fr/euprunusdb/

EPDB Portal

0.1

(1) Durone di Cesena I (4) Noir de Meched (3) Cristallina (2) Cuore Aspromonte (2) Vallescura II (2) Maiatica (2) Vallescura I (4) Goodnestone Black (4) Colney (3) Quarantana (1) Durella II (3) Kronio (1) Duroncino (1) Durone Nero I (4) Noble (1) Durone Compatto Vignola (1) Durone di Cesena II (1) Durone Nero II (3) San Pitrisa Zappia (2) Grammendula II (1) Durona Sangiovanni (1) Cornetta I (1) Cornetta II (1) Fiore I (1) Fiore II (1) Durella I (1) Morandina (2) Amarena grecanica (1) Morena (3) Cappuccia Castelbuono (3) Carrubbedda (2) San Pietro San Lorenzo (2) Zuccaredda (3) Caddusa (3) Itala S. Mela (2) Maiatica rossa (3) Moscatella Chiusa Scalfani (3) Maiolina S. Mela (2) Piede Corto (3) Nireddi (3) Puntalazzese (2) Carraffune bianco (2) Lombardune (3) Niredda Cappuccia (2) Semenzale Morbido (1) Gemella (3) Furticchiara (4) Napoleon (2) Grammendula I (3) Cappuccia Imperiale (2) Iancuzza Napoletana (2) Carraffune (3) Raffiuna S. Mela (2) Abenavoli bianco (2) Zuccarigna (2) Bombardune (2) Carrammendula II (2) Moddacchia Aspromonte (3) Nucigliera (3) Sant'Angelo S.Mela (2) Citra (2) San Pietro Cardeto (2) Cappucciarica (2) Cappucciarica San Lorenzo

54 distinct profiles

17 non-distinguishable profiles

2 to 15 alleles/SSR locus (mean = 7.6);

He range 0.37-1.00 (mean = 0.77);

PIC range 0.38-0.75 (mean= 0.60);

Most polymorphic SSRs in the set:

BPPCT037 (15) and PceGA34 (12)

Least polymorphic:

EMPA002 (2), EMPA003 (4) and EMPA017 (4)

Genetic diversity assessment

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

45 Italian accessions (37 unique genotypes)

Cvs. of known S-genotype

amplification of S-RNase and SFB genes

0,00

0,03

0,06

0,09

0,12

0,15

0,18

Fre

qu

en

cy

13 S-alleles detected

17 groups represented

5 ‘new’ groups

2 universal donors

1 self-compatible (S5’)

(In)compatibility group assignment*

S3, S6 , S13 and S16 are well represented across Italian germplasm;

S1, S2 are rare;

S4 is absent (the only accession carrying S4 is most probably a synonym of Napoleon);

*according to Tobutt et al. (2004); Marchese et al. (2007), Schuster et al. (2007), Ipek et al. (2011).

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

Future breeding goals

Self-compatibility

Environmental adaptability

low chilling requirement

Extended ripening season

very early, late

Better fruit quality

good taste

high flesh firmness

high sugar content

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

Availability of diverse germplasm as a source of useful traits

Kronio self-progeny

People

Thanks for your attention

WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference

Daniela Giovannini

Annalisa Leone

Sandro Sirri

Alessandro Liverani

Franco Marra

Annalisa Marchese

Antonino Raimondo

Tiziano Caruso

Rocco Mafrica

Sonia Pangallo

Paolo Pellegrino