FigureS1.Gandhirajanetal A B Control LPS BTP2...
4
% Nuclear NFATc3 GFP Control LPS BTP2 LPS+BTP2 *** *** Wild-type Control LPS BTP2 LPS+BTP2 A Figure S1. Gandhirajan et al B
Transcript of FigureS1.Gandhirajanetal A B Control LPS BTP2...
%N
ucle
arN
FATc
3G
FP
Control
LPSBTP2
LPS+BTP2
*** ***
Wild
-type
Control LPS BTP2 LPS+BTP2AFigure S1. Gandhirajan et al
B
LPSUntreated
Hyp
er(f.
a.u.
)
**
Untreate
dLPS
LPS+DPI
LPS+EGTA
LPS+BTP2
(F/F
0)
B
** ******
Untreate
dLPS
Untreated LPS
CLPS+DPI
LPS+BTP2
Time (s)
Untreated LPSA
0
4096
LPS+EGTA
[Ca2+
] c(F
Fluo
-4;f
.a.u
.)H
yPer
Cyt
o
Osc
illat
ions
(min
- 1)
Time (s)[Ca2+
] c(F
Fluo
-4;f
.a.u
.)
D E F
G H I
[Ca2+
] c(F
Fluo
-4;f
.a.u
.)[C
a2+] c
(FFl
uo-4
;f.a
.u.)
LPS
*
LPS+DPI
LPS
Osc
illat
ions
(min
- 1)
Untreate
d
*
J
K
LTime (s)
Time (s)
[Ca2+
] c(F
Fluo
-4;f
.a.u
.)
MEF untreated A549 untreated J774A.1 untreated
Time (s)
MEF A549 J774A.1
J774A.1 + DPI J774A.1
Figure S2. Gandhirajan et al
untreate
d wt
gp91phox -/-
InsP 3R
II-/-
Stim1∆
EC
LPS
gp91phox -/-
F
LPSwt
[Ca2+
] c(F
Fluo
-4;f
.a.u
.)Untreated
A
InsP3R II -/-
LPS
Time (s)
LPSwt
Stim1∆EC
(F/F
0)
***
LPS (1 µg/ml)
[Ca2+
] c(F
Fluo
-4;f
.a.u
.)
Time (s)
***
B C
D E
Figure S3. Gandhirajan et al
B
Unt
reat
ed
gp91phox -/-Wild-type STIM1 KD
LPS
STIM1
STIM1 shRNA
Wt 1 2 3 4 5
ββ actin
AFigure S4. Gandhirajan et al
