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Angela SanchezMentor: Pamela Larsen

Inhibition of temperature-regulated proteostasis genes causes an early onset progressive dysfunction in motorneurons

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Cool temperature extends lifespan in C. elegans

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Cool core temperature longevity is conserved

Drosophila

30c18c(Miquel 1976)

Mouse

(Conti 2006)

(Hunter 1999)Ames Dwarf

(Lane 1996)Caloric Restriction

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Cool temperature is neuro and cardio protective Enhance survival after stroke (Kammersgaard LP J Am Heart Assoc 2002)(Roth 2002)

Enhance survival after heart attack (Varon J Chest 2008)

32C37C33C 37C(Campos 2012)(Chenoune 2010)

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Universality of cool temperature improving health and survivalUnderlying mechanism not understood

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Approach to understanding healthy long-term survivalMicroarrayAffymetrix C. elegans array

Genome wide snapshot

UNBIASED

PRIMARY biological response to a chronic change

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Primary cause occurs prior to evidence of aging

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Experimental design of samples for microarrayL1L2L3L4Immature Adult

Development Total RNATotal RNAMature Adult Visible Developmental Stage 15C15C25CFour biological samples

Explain that we are starting at the beginning (day one chronic response) acute is what saves you in a pinch but chronic is what correlates with lifespan acute is not tolerable long-term8

Temperature regulated genes

19,762 Predicted Genes

16,896 Probes383 Differentially Expressed179P