2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20
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Instrument Name: DE24802783 Firmware: Serial#: Assay Information: C.01.069 DE24802783 Instrument Information: Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100 expert\assays\RNA\Eukaryote Total RNA Nano Series II.xsy Assay Class: Version: Assay Comments: Eukaryote Total RNA Nano 2.6 Total RNA Analysis ng sensitivity (Eukaryote) © Copyright 2003 - 2009 Agilent Technologies, Inc. Chip Information: Chip Comments: Type: G2938B Chip Lot #: Reagent Kit Lot #: C1 RIN: 4.10 C2 RIN: 9.20 C3 RIN: 8.80 BC1 RIN: 2.50 BC2 RIN: 9.20 BC3 RIN: 8.50 SN1 RIN: 9.90 SN2 RIN: 7.30 SN3 RIN: 9.50 V1 RIN: 8.50 V2 RIN: 9.80 V3 RIN: 7.90 2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14 2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of 1 17 Created: Modified: 10/08/2011 15:29:20 10/08/2011 15:53:27 Data Path: Eukaryote Total RNA Nano C:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Assay Class: Electrophoresis File Run Summary
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Transcript of 2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20
Instrument Name: DE24802783 Firmware:Serial#:Assay Information:
C.01.069DE24802783
Instrument Information:
Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100expert\assays\RNA\Eukaryote Total RNA Nano Series II.xsy
Assay Class:Version:Assay Comments:
Eukaryote Total RNA Nano2.6Total RNA Analysis ng sensitivity (Eukaryote) © Copyright 2003 - 2009 Agilent Technologies, Inc.
Chip Information:
Chip Comments:
Type: G2938B
Chip Lot #:Reagent Kit Lot #:
C1RIN: 4.10
C2RIN: 9.20
C3RIN: 8.80
BC1RIN: 2.50
BC2RIN: 9.20
BC3RIN: 8.50
SN1RIN: 9.90
SN2RIN: 7.30
SN3RIN: 9.50
V1RIN: 8.50
V2RIN: 9.80
V3RIN: 7.90
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of1 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electrophoresis File Run Summary
Sample Name Sample Comment Status Result Label Result ColorC1 RIN: 4.10C2 RIN: 9.20C3 RIN: 8.80BC1 RIN: 2.50BC2 RIN: 9.20BC3 RIN: 8.50SN1 RIN: 9.90SN2 RIN: 7.30SN3 RIN: 9.50V1 RIN: 8.50V2 RIN: 9.80V3 RIN: 7.90
Chip Lot # Reagent Kit Lot #
Chip Comments :
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of2 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electrophoresis File Run Summary (Chip Summary)
Ladder
Overall Results for LadderRNA Area: 171.4 RNA Concentration: 150 ng/µl
Result Flagging Color:Result Flagging Label: All Other Samples
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of3 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary
Fragment table for sample 1 : C1Name Start Time [s] End Time [s] Area % of total Area18S 41.71 42.93 5.4 2.428S 48.90 50.92 4.8 2.1
C1
Overall Results for sample 1 : C1RNA Area: 227.7 RNA Concentration: 199 ng/µlrRNA Ratio [28s / 18s]: 0.9
RNA Integrity Number (RIN): 4.1 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 4.10
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of4 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 2 : C2Name Start Time [s] End Time [s] Area % of total Area18S 41.10 43.70 29.3 16.728S 47.46 51.73 60.6 34.4
C2
Overall Results for sample 2 : C2RNA Area: 176.0 RNA Concentration: 154 ng/µlrRNA Ratio [28s / 18s]: 2.1
RNA Integrity Number (RIN): 9.2 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 9.20
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of5 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 3 : C3Name Start Time [s] End Time [s] Area % of total Area18S 41.06 43.01 25.1 14.928S 48.66 51.20 42.9 25.4
C3
Overall Results for sample 3 : C3RNA Area: 168.7 RNA Concentration: 148 ng/µlrRNA Ratio [28s / 18s]: 1.7
RNA Integrity Number (RIN): 8.8 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 8.80
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of6 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
BC1
Overall Results for sample 4 : BC1RNA Area: 136.7 RNA Concentration: 120 ng/µlrRNA Ratio [28s / 18s]: 0.0
RNA Integrity Number (RIN): 2.5 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 2.50
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of7 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 5 : BC2Name Start Time [s] End Time [s] Area % of total Area18S 40.61 43.44 21.1 15.028S 47.17 51.70 48.2 34.3
BC2
Overall Results for sample 5 : BC2RNA Area: 140.5 RNA Concentration: 123 ng/µlrRNA Ratio [28s / 18s]: 2.3
RNA Integrity Number (RIN): 9.2 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 9.20
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of8 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 6 : BC3Name Start Time [s] End Time [s] Area % of total Area18S 40.90 43.33 18.6 13.928S 48.00 51.05 35.2 26.3
BC3
Overall Results for sample 6 : BC3RNA Area: 133.8 RNA Concentration: 117 ng/µlrRNA Ratio [28s / 18s]: 1.9
RNA Integrity Number (RIN): 8.5 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 8.50
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of9 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 7 : SN1Name Start Time [s] End Time [s] Area % of total Area18S 40.90 43.31 30.9 19.028S 46.35 51.37 74.3 45.7
SN1
Overall Results for sample 7 : SN1RNA Area: 162.6 RNA Concentration: 142 ng/µlrRNA Ratio [28s / 18s]: 2.4
RNA Integrity Number (RIN): 9.9 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 9.90
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of10 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 8 : SN2Name Start Time [s] End Time [s] Area % of total Area18S 40.07 42.51 22.3 10.228S 47.54 50.32 32.5 14.9
SN2
Overall Results for sample 8 : SN2RNA Area: 217.3 RNA Concentration: 190 ng/µlrRNA Ratio [28s / 18s]: 1.5
RNA Integrity Number (RIN): 7.3 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 7.30
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of11 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 9 : SN3Name Start Time [s] End Time [s] Area % of total Area18S 40.80 42.97 31.8 17.828S 46.73 50.84 71.5 40.0
SN3
Overall Results for sample 9 : SN3RNA Area: 178.7 RNA Concentration: 156 ng/µlrRNA Ratio [28s / 18s]: 2.3
RNA Integrity Number (RIN): 9.5 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 9.50
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of12 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 10 : V1Name Start Time [s] End Time [s] Area % of total Area18S 40.46 42.85 41.1 15.228S 47.74 50.24 64.9 24.0
V1
Overall Results for sample 10 : V1RNA Area: 270.8 RNA Concentration: 237 ng/µlrRNA Ratio [28s / 18s]: 1.6
RNA Integrity Number (RIN): 8.5 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 8.50
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of13 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 11 : V2Name Start Time [s] End Time [s] Area % of total Area18S 41.28 42.79 62.8 19.028S 47.16 50.59 142.7 43.2
V2
Overall Results for sample 11 : V2RNA Area: 330.5 RNA Concentration: 289 ng/µlrRNA Ratio [28s / 18s]: 2.3
RNA Integrity Number (RIN): 9.8 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 9.80
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of14 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
Fragment table for sample 12 : V3Name Start Time [s] End Time [s] Area % of total Area18S 40.62 42.89 16.9 11.828S 47.57 50.34 24.7 17.3
V3
Overall Results for sample 12 : V3RNA Area: 143.1 RNA Concentration: 125 ng/µlrRNA Ratio [28s / 18s]: 1.5
RNA Integrity Number (RIN): 7.9 (B.02.07) Result Flagging Color:Result Flagging Label: RIN: 7.90
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of15 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Electropherogram Summary Continued ...
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of16 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Gel Image
Standard Curve
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 10/08/2011 16:46:14
2100 expert_Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad Page of17 17
Created:Modified:
10/08/2011 15:29:2010/08/2011 15:53:27Data Path:
Eukaryote Total RNA NanoC:\...Eukaryote Total RNA Nano_DE24802783_2011-08-10_15-29-20.xad
Assay Class:
Curves
