20111129 biopod page 1 4 A3 - FOGALE nanotech · The bioPOD multi fermentor platform can be used to...
Transcript of 20111129 biopod page 1 4 A3 - FOGALE nanotech · The bioPOD multi fermentor platform can be used to...
BIOPOD seriesThe Protein Machine on the Bench
What makes the difference ?What makes the difference ?
Expandable with No Compromise Do it all Faster and Better
Access Incredible Benefits with the BIOPODThe protein machine on the bench
Proven Scalable Results
Scalability to larger conventional pilot fermentor has been proved with similar
sensors, parameter control ability and performance.
The bioPOD multi fermentor platform can be used to scale-up or scale down
existing fermentation models (process optimization) with biological recipes built
in the system, customised or other define biological model implemented into the
software platform.
The Advantages
Compare to traditional scale-up or scale-down operation in fermentation, the biopod platform is offering significant
advantages:
- The fermentation is driven by a remote biological model
- Insight and Improvement of the robustness of a process ( for PAT and Quality by Design initiatives)
- Independent fermentor to study at same time different biological strategies and enhance the production of the
targeted protein
- New design of recipes is validated before use in routine conditions by the end user
- Culture are started any time without an operator when fermentor are used in an remote mode with a built in recipe
Introduction
The challenge for Process Development, Media optimization, Process optimization or clone screening lies in the limited
performance of available equipment using traditional scale–up or scale-down methods and equipments at bench scale. Culture
model as shake flask, well plates or disposable multi reactors do not provide so far the automated biological culture models or
recipes of culture to get faster results. The bioPOD is at bench scale, representative of a true scale up method and recipes able to
be transferred at a manufacturing stage with biological control on the process.
Reduce and Optimize R&D and Process Development Efforts
The bioPOD is a multi fermentation platform (POD) which enable multi-factorial fermentation runs based on
built-in biological recipes of culture for aerobic or anaerobic fermentation. The existing built-in recipes are
capable of performing fully automated batch, fed-batch or chemostat processes with pre-defined standard
culture recipes but also able to be customized due to the modularity of the software platform.
Compact Modular Design
Available in single, twin, triple and up to 64 units, the bioPOD design is based on the use of an ergonomic,
modular construction housing the local controller as well as additional gassing, pump and various modules.
This result in considerable savings in valuable benchtop space.
The powerful local controller built in a tower arrangement or separate as modules (shelf stackable) offers a
comprehensive range of control and display facilities, like as up to 9 automated biological recipes and for
classic remote control cascadable PID control loops, x-stage cascade control for pO2, alarm monitoring, recipe
control and password protection for critical system parameters
In addition there are software modules like specific biological
recipes customized and customizable to the targeted protein to
be expessed:
In combination with its software capabilities, the bioPOD is a
concept fully designed to change the way fermentor
performance must be achieved with less running time and less
complexity.
Unique Benefits
Modular and Scalable from 100 ml up to 10 L Working Volume
Biological Recipes Monitoring the Fermentation Processes
Optical and capacitance sensors Coupled for enhanced fermentation control
Fully Remote Process control customizable to Specific Fermentation Needs
Ready to Use Induction models built in the Software Package
Easy Connection to Sensors
Simple Modular Design on the Bench or Wall Mounted
Dual Peltier module with Built-In OD Sensors
Advanced Software
The software platform is designed to support biological models based on physical parameters
to control automatically the growth of cells.
Basically designed for protein production with E. coli the bioPOD can be also used with any
bacteria strength and yeast such as Pichia Pastoris or Saccharomyces.
This innovative and unique method of supervision lead to a concept where cells are managing
the process instead of using only the physical parameters as the way to manage and control a
fermentation run.
Data storage of graphs and recipes with retrieval functions for an easy access to existing data.
F200 unit with 2 x 100 ml bioreactors
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Vessel Biopod serie F200 F1 F3 F5 F10
Min Working Volume (liter) 0,03 0,2 0,4 1 3,5
Max Working Volume (liter) 0,1 1 2,9 6 11
Material Borosilicated Glass, 316 L S/S
Control unit Control Panel Color Touch Panel
Calibration Local Easy Interface
Communication Port RS 485
Program Storage Full Embedded Software
Log Data Storage Remote Controlling Software.
Cabinet material ABS
Dimension (inch) 14.6'' x 28.8" x 9.1" (W x H x D)
Aeration Inlet Gas Flowmeter TMFC for Air/O2 up to 2.5 VVM
Sparger Sintered
Temperature Control System Chiller, Heating Belt
Range 4 or 14 C up to 65 C
Resolution 0,1 C
Probe Platinum RTD Probe (PT100)
Control mode Manual, Auto (PID), Remote
Agitation Drive Removable Top Brushless Motor
Speed Range 30 – 1200 RPM
Resolution 1 RPM
Impeller 1,2 or 3 Rushton Impellers
Control mode Manual, Auto
F200 system Gas lift
pH Range 2 – 12 pH
Resolution 0,01 Unit
Electrode Gel Filled, Autoclavable
Control Mode On/Off, Adjustable PID 1Way, 2 Ways, Manual, Auto, Remote
DO Range 0 – 470 % (100% for air)
Resolution 0,1 %
Probe Polarographic or optical, Autoclavable
Control Mode Adjustable PID, Cascade, Manual, Auto, Remote
OD Range 0 – 4 AU
Resolution
Probe Optical, Autoclavable, USP Class VI Materials.
Control Mode Adjustable via Recipe Control
F200 system Integrated external sensor
Capacitance Range 1g/L – 200g/L DCW for Yeast, Bacteria and Fungi
5g/L min with Highly Aerated Media
0 - 700pF/m Max Permittivity; 0.5 – 100mS/cm Conductance
Resolution 0.02g/L DCW for Yeast
Probe Capacitive, Autoclavable, USP Class VI Materials.
Control Mode Adjustable via Recipe Control
Foam Probe 316 L S/S Height Adjustable
Control Mode ON/OFF Control
Peristaltic pumps Pump number 6 Built-in Pumps, 2 External pumps
Motor Type Pulse Driven Motor for
Flow Rate minimum 0.15μL each 46h up to nominal flow rate.
Speed 47 RPM, 100RPM (other on request)
Nominal Flow rate 3.2ml/min (built in), 75ml/min (external). (other on request)
Resolution 0.15μL
Control mode On/Off, Manual, Auto, Remote
Pump(s) can be Assigned for Acid, Base, Inoculum, Inducer, Anti-Foam and Substrates
Off Gas Device type Glass or 316 L S/S Condenser
Utility requirement Power Supply 110V or 230V; 50/60 Hz with Safety Cut off Switch
Water No Water supply Connection with chiller
Gas 1.2 bar Max. Medical Grade, dry, oil free and filtered
Specifications
BIOPODThe protein machine on the bench
Europe FOGALE nanotechNîmes, FrancePhone: +33 (0)4 66 62 05 [email protected]
USA / Asia FOGALE biotech, Inc.Cambridge, MA 02140Phone: (818) 206 [email protected]