FRANK E. INSCORE (Curriculum Vitae)

(Home) 53 Lufkin Lane, Bristol CT 06010 860.899.4943 feinscore@gmail.com www.linkedin.com/in/finscore2014

RESEARCH and DEVELOPMENT PROFESSIONAL Director & Manager / Scientist & Chemist / Leader & Innovator

Visionary, high-performing senior level R&D professional offers over 15 years in cutting-edge research, strategic planning, program management, new application & product development, and customer interface

solid practical experience as an energetic take-charge hands-on lead scientist of unquestionable loyalty integrity, with a broad scope of analytical chemical experimental laboratory technical and management expertise, who leads by example (informally or by authority), works well in groups or independently, and drives innovation in cross-functional / matrix environments

experienced synthetic chemist in multistep/total synthesis, reaction methodology development, bioorganic, organometallic chemistry and catalysis, plus verifiable use and understanding of advanced organic and inorganic synthesis/purification/characterization techniques; + expert sol-gels/processing, nano-materials/coating

∗ Top performer who can evaluate organizational/departmental issues or needs; ID lucrative growth opportunities ∗ Integral leader who excels in optimization; includes conceptualizing creating and implementing forward-thinking programs projects processes toward new innovation & discovery to achieve continued key growth & profitability ∗ Prof. manager who delivers success via ability to adapt +building/leading/inspiring teams to realize performance ∗ Proven organizer/planner who is attentive to details; effective interpersonal skills, superb communicator listener ∗ Valued problem-solver who is analytical, creative, flexible; open to new ideas, not adverse to risk, but pragmatic ∗ Possess investigative mindset; digest large complex volumes of information, summarize/synthesize/disseminate ∗ Passionate researcher eager to contribute in all endeavors, leverage advanced skills and knowledge to succeed

PROFESSIONAL PROFILE (QUALIFICATIONS) ∗ Ph.D. in Chemistry (MS/BS/BA across the chemistry-fields/biological-sciences math/physics/comp-sci. behavior) ∗ Plus13 yrs high-profile high-impact research, new technology application product method development, Mgmt 3yrs postdoctoral bioorganic methodology/synthesis, molecular models; structure/function, enzymatic catalysis 10yrs direct/led industry R&D teams/programs/projects; synthesis nanomaterial/sensing-devices new products ∗ Extensive exposure in design construction buildup of analytic/instrument/synthetic/biologic/nano-material labs ∗ Expert in current analytical methods, cutting edge synthesis, advanced purification/characterization techniques ∗ Expert in realizing new applications / innovative-solutions; applies multidisciplinary/spectroscopic approach ∗ Skilled in Project and Program Management methods; R&D Management System / New Product Development Idea & Innovation Mgmt, apply to leadership and PM for controlled sustainable approach to innovative projects ∗ Exceptional science/technical knowledge enables superb planning/execution; sustain strategy/remains attentive ∗ Fosters relationships/cross-functional collaborations across industry/government/academia/investment sectors Targeted markets - DoD/DHS/law/food/forensic/environment/med-health/biotech/pharm/chem/petro industries ∗ Visionary strategist excels in innovative solutions, strong negotiator and influencer with verifiable achievement record creating developing new R&D strategies and program plans resulting in explosive growth/biz profitability ∗ Dedicated; desire and commitment to succeed, see accomplishment driven career, tenured record of increasing responsibility, rapid advancement, rewards ∗ Productive; see management & research record, 50+ programs projects contracts completed on time / in budget providing over $20 million in revenue, 48 publications, 50 presentations (+supported 50), 4 patents (+assisted 4) Results show clear evidence of exemplary problem-solving / organizational / verbal written communication skills

PROFESSIONAL EXPERIENCE

R&D Consultant (New Product & Application Development Scientist, Chemist), Hartford CT 2013 - present • Engaged as private consultant; leveraging progressive knowledge/skills for success, New materials / processes for New applications / products; e.g. medical devices / sensors for biomarker / drug analysis in clinical samples

• Developed new business relationships via online network by promoting credentials as technical expert & leader over diverse scientific disciplines, with proven track record for strict confidentiality of client / project details through NDAs; increased contact base from 500 to 2000 in 12 months, 48 new technical consultations - global recognition as analytical spectroscopy specialist led to business discussions - market drivers targets/cost - invited to sit on executive board of new start-up based on material research, chemistry, spectral library expertise - recognized by peers world wide, accepting editorial board seat on SOJ Biosensors Biomarkers and Diagnostics • Performed self-assessment to address core competencies, expanded capabilities in software & program project management methodologies to meet future R&D challenges and diverse needs in changing global environment • Organize analyze data for reports articles patents proposals presentations; e.g. continued bioagent assay work with RTA & US Army ECBC led to publication & front cover of Analyst 12/21/14 • Adapted industrial manager leader skills as STEM science technology engineering mathematics School mentor • Designed & tested consumables - sampling kits to collect / treat fluid or solid for rapid target release / extraction - continued comprehensive analysis of real street drug samples, such as bulk / surface / forensic powder cocaine measured by Raman-SERS & subsequent measurements of clinical biofluid samples (e.g. saliva) collected from patients before & after consuming known quantities of bulk (e.g. via nasal inhalation) over 24hr period by SERS - optimized method development & validated results, by parallel analysis with standard GC-MS HPLC procedures - discriminated powder samples with respect to purity - nature / number adulterants, by Raman chemometrics

Principle Scientist / Director of R&D Real-Time Analyzers, Inc. Middletown CT, 2006-2013 promoted to Director based on success leading team/managing complex programs projects; reporting to CEO/VP empowered as R&D Director (dept) to identify/implement new initiatives towards accelerating growth & profitability 2008 assumed expanded R&D executive leadership role/function of Chief Scientist (organization) to facilitate this.

performed dual hands-on role as Principle Investigator or Sr. Program Manager for 20+ R&D contracts initiated independently as PI or collaboratively as coPI/CI. Leveraged acquired skills built upon previous positions

developed R&D organization strategies/strategic initiatives/operating plans to achieve company mission / goals ∗ collaborated in R&D organization Strategy / Strategic Plan development; plus directed Program Planning, while maintaining continuous alignment of program scope with strategic objectives and business goals ∗ drove multiple programs to ensure budget/timeline/quality/staffing & other project goals / milestones met ∗ e.g. 2010 directed 8 complex concurrent programs/projects; delivering results in this fast-paced deadline driven environment; success achieved by ability to multi-task, effective prioritization organization and a well-trained team

leveraged R&D efforts in development of new revenue streams and innovative products ∗ very productive output; increased number new clients, sales, deliverables; increased revenue & profit ∗ developed new types analyzers/sensors, complete solutions; new applications with private/public sectors

directed led R&D to identify create develop new relations/collaborations/markets; expand product pipeline ∗ e.g. business relationships with INESCO, Kraft Foods, Colgate-Palmolive; new contacts, contracts, revenue ∗ e.g. collaborations with Harvard Medical School researchers, Army ECBC; new applications/expand technology ∗ e.g. initiated global market entry, SERS-ID; chemicals Swedish DoD/airports, drugs UK Police/roadside ∗ e.g. opened up profitable markets with new innovative bioagent/pathogen SERS-based assays, funding/patent ∗ e.g. independently developed commercialized 1st SERS Lib publicly available; value add-on for analyzers

drove R&D - led teams in acquiring new funding/contracts - to develop & commercialize new products ∗ 13 new SBIR-programs/private-contracts, initiated formulated independently as PI ∗ additional $7.5 million secured, doubled #contracts, improved Phase I to II conversions from 55% to 90% ∗ typical: timelines 6mo to 4yrs, budgets $75K - $1.3 million, staff/project 1-8; efficiently effectively managed

provided support as expert-consultant/top-scientist/troubleshooter representing company internally/externally ∗ tracked scientific community/papers, technical developments/patents/product launches/market trends in industry ∗ drove presence via publications, presentations conferences tradeshows, professional associations/groups ∗ recommended new research opportunities/technological ventures; supported new marketing initiatives/sales

combined leadership & management skills to build develop maintain world class R&D team / culture ∗ served represented company; to actively seek-out recruit top R&D talent, immediate/long range staffing plans ∗ developed qualities of team members; performed annual reviews, use of individual development plans ∗ conducted meetings on regular basis to provide feedback recognition of team accomplishments/teamwork ∗ provided clear vision to goal (members embrace product’s vision strategy business goals) motivated team ∗ empowered team members with varying degrees of responsibility; improved confidence, job satisfaction ∗ Result, highly-productive loyal 12-member cross-functional team, and future leader source

utilized R&D Management System, directing/managing R&D process towards New Product Development ∗ implemented this process model for taking new products from conceptualization to commercialization ∗ used modifiable R&D-NPD template developed in-house; full documentation in our research proposals ∗ performed feasibility studies, plus inclusion of phase-gate (stop/go) decision points; e.g. for private projects ∗ e.g. output - new prototypes/deliverables, complete systems for detecting analyzing chemicals and biologicals

collaborated in Product Development with Product Lifecycle Management / Product Life Cycle Mgmt. ∗ provided R&D/NPD interface with engineering/marketing/sales/manufacturing; engaged marketing through NPD ∗ directed coordinated R&D resources in cross-functional matrix, helped plan action items/milestones/schedules ∗ e.g. Portable Fuel Analyzers (prototype/final validated product) delivered to USMC, with demilitarized versions commercialized marketed for public, end result of PLM/PLCM plan; other roles + led fuel analysis/chemometrics + benchmarked analyzer performance, defined settings-optimal system response, best preprocessing method + established specifications, design optimize analysis/decision-tree, stream-lined user interface, ASTM validated

supported Production both internal/external, analyzed raw materials-inputs/intermediates/process-outputs ∗ e.g. pharmaceutical drugs/ APIs/ excipients /counterfeits, with FT-IR/MIR/1064-Raman/785-SERS; Infratac ∗ e.g. developed complex multistep synthesis new proprietary drugs for a law firm, original patent just expired, only small window of opportunity to submit new patent; required new precursors/intermediates; Successful ∗ e.g. analyzed by SERS, chemicals in new tobacco line/any carcinogens produced if smoked, R.J. Reynolds ∗ e.g. analyzed by FT-Raman (at 1064nm/785nm), fluorescent polyolefin formulations for Chevron Phillips ∗ e.g. petro products; raw materials/fuel components, crude oil/blends, refined fuel/oil/fluid/synthetics, chemicals 1064-FT-Raman / ASTM std. methods - Parameters: TBP-distillation/distillation/kinematic viscosity/flash-point pour-point/acid number/density/ total sulfur/cloud-point/aromatics; LIB 2000+ unique fuel/blend/nonfuel samples PCA/PLS/DA/SIMCA to ID/discriminate/predict-props of fuels/contaminates: jet/diesel/gas/ft-sasol/biodiesel/mgo

enhanced existing products/processes, improving bottom line (e.g. in SERS-substrate sensor fabrication) ∗ designed clean room modules, authored SOPs, part of QMS, to standardize/automate steps in sensor mfg. ∗ included optimization/refinement, all steps in precursor / sol-gel / nanoparticle / substrate preparation + applied experimental design methods to implement valid efficient experiments, in process optimization + used organic process methodology with break-down process optimization; added value - scalable/purity + assessed all system components; identify resolve any compatibility purity stability degradation issues e.g. revealed advanced fluorinated-polymer materials/wares necessary to ensure no leaching at conditions ∗ improved chemistry/packaging; less contaminants, more reproducible, shelf-life 2wks extended to 3months ∗ benefit, sensor Quality improved by removing variations from production processes; plus reduced waste ∗ net effect; cut product replacement cost 25%/decreased losses due to returns shipping faulty sensors 95% cut time to shipping by 50%; initiated customer follow-up for feedback, satisfaction ~98% with new sensors

continued development of technology/applications expanding building from previous results/innovations ∗ developed unique materials, nanocomposites, new methods; extract detect target analytes in various media

need to explore new materials for more efficient cost effective means to manufacture chips

fabricated poly(methyl methacrylate) chips, investigate PMMA / UV-cure initiator benzoin methylether (BME) best at 60 ºC, stir for 4min to initiate curing used poly(dimethyl siloxane) as more efficient cost effective substitute to manufacture chips / lab-on-chips PDMS LOC substrates too porous/leaky, not compatible with our strong sodium borohydride reducing agent replaced PDMS by a silicone, a two-part solution (Dow Corning Sylgard 184 silicone / elastomer) after mixing see cure in ~ 48 hours at room temperature, or in ~ 1 hour at 100 ºC etched channels on silicon-wafer micro-chip and on glass-plates; collaborative effort - RTA/NASA/ASTID/JPL best approach but expensive, time-consuming, complicated manufacturing process loaded Ag-doped MTMS sol-gel plug into length of PFTE (poly(fluoro tetraethylene)) tubing, cure and reduce binding/sensitivity good; but plastic flexibility/bending affects reproducibility along sol-gel plug axis

need innovative cure method towards useful application of our new coating formulations / technology; chips applied UV curing (photochemical process) as alternative to conventional heat/air-drying, e.g. instantly cure dry coatings or adhesives with advantage production speed, improved properties, bonding; contrast, to solvent evaporation, pollutants, coat thickness loss, volume loss due to shrinkage e.g. used adhesives such as polyurethane, and epoxies, for manufacturing chips, sealing components; lock threads against vibration, secure instrument components

need innovative reduction method towards useful application of our SER-active sol-gels/new coating formulations applied UV light as alternative to wet chemical borohydride reduction that is difficult to control, not uniform with messy byproducts; advantage simple, more consistent, amenable to mass production and long shelf life; e.g. could uniformly simultaneously reduce blocks of gold SG microplate wells; optimized process by DOE varying wavelength, intensity, distance, cure depth; power duration repetition, order, for given chemistry; contrast, reproducibility and stability less in borohydride reduced microplates, prone to spectral artifacts

need universal all-purpose alternative to our Ag SG chemistries; more active/sensitive over entire category/class synthesized developed outstanding gold-SG chemistry; robust, easy to use, easy to manufacture in quantities e.g. could detect identify 300 drugs/metabolite; priority abused drugs from saliva ~100-1 ng/mL (100-1ppb)/8min contrast Ag SG chemistries at best ~175 drugs detected, with sensitivity ~100,000 -1000 ng/mL (100-1ppm)

need new materials/methods to provide specificity - identify discriminate spores/bacteria at genera species levels led development new hybrid nanomaterials/functionalized nanoparticles; SER-based bioagent/pathogen assays synthesized novel SG capture matrix/immobilized Ag Au nanoparticles/functionalized with peptides/antibodies highly-specific molecular recognition elements; developed assay steps to improve sensor selectivity sensitivity e.g. could detect single-digit Bacillus anthracis spores in under 15-min; assay discriminates B. cereus spores contrast, without functionalization, all species Bacillus spores would give same undifferentiated DPA SERS

need new nanomaterials incorporating functionality to modify polarity hydrophobicity, improve physical properties applied sol-gel process to form polymer-inorg hybrid nanocomposites (PDMS, polypropylene & other polymers); prepared organo-functional silanes, bind polymer (e.g. PEG), and incorporate directly into sol-gel structure e.g. used in-situ sol-gel method with metal nanoparticles, yielded new polymer - inorganic Ag or Au NP hybrids contrast, just mixing non-surface reactive polymer into sol-gel process, is less effective, inhomogeneous

need new chromatographic materials apply; small samples in our capillaries/more economical control particle size developed new IEX/SPE sol-gel based materials - separation/purification in 800-200nm i.d. capillaries/channels e.g. SG-based anion-exchange materials for coating inner surface of capillaries using N-octadecyldimethyl[3- (trimethoxysily)propyl]ammonium chloride with TMOS and TFA catalyst; see bisphosphonate drugs 10ppb e.g. used similar approach with polymer-modified sol-gels, flow 10-0.01mL samples, detect 50 pesticides/10ppb contrast, without flowing or use of such IEX materials that can pre-concentrate isolate, sensitivity ~1 ppm

need standard method for reliable performance evaluation and comparison of our SER-substrates with others led team synthesized/characterized new nanosubstrates/structures +reported e.g. fused Ag Au on Si spheres, nanorods various colloids, functionalized nanoparticle/Si surfaces, commercial Klarite, Van-Duyne FON/pillars; e.g. used ROC analysis/calculated enhancement; showed our hot-spot SERS substrates to be more sensitive; results published - publicity increased sales / industry presence contrast, without standard method, comparison biased w/r to substrate / sample / measurement conditions

leveraged innovation, additional funding based on extension to new applications ∗ developed SERS to measure bioagents and pathogens; SERS-based assays NSF P1/P2/P3 as PI, detected Category A/B bioagents spores/bacteria/toxins in water/fluid/aerosol/surface e.g. P1 led to following three 3rd party investment/commercialization opportunities; two additional SBIR fund

∗ Kraft Foods, R&D contract, PI, same technology applied to food borne pathogens Listeria/Salmonella value - added antibody capability; how to functionalize metal particles with antibodies ∗ Colgate-Palmolive, 2 R&D contracts plus an analyzer purchase (identical to prototype built), as PD value - added tooth decay bacteria detection capability, customer feedback improved detector /software ∗ Viagen, R&D contract, cPI/PM, use of SERS plate reader to detect embryo viability value - added chemometric capability to SERS analysis (differentiate viable non-viable livestock embryos) ∗ DARPA P1/P2, PI, anthrax, bubonic plague (Y. pestis) botulism (Clostridium) tularemia (F. tularensis) assays e.g. used ROC validated system performance prototype SERS-capture assay P2 (microchip/microplate) URI ∗ NSF P1(P2) food-borne pathogens as coPI, feasibility study bilayer-membranes e.g. developed filter-microplate for bidirectional high flow/throughput assays, new product great potential

e.g. leveraged investments/obtained NSF-P2B matching funds, field-testing ECBC Biohazard Level 3

∗ developed SERS to measure drugs metabolites and biomarkers in biofluids NASA P2, as PI, prior success with SERS capillaries to measure biomarkers in urine indicative of bone/muscle loss and renal stone formation, plus drugs to treat these deleterious effects, e.g. led to $1.2 million UK contract

∗ UK Home Office R&D contract P1/P2/P3, as PI, develop road-side drug-in-saliva analyzer prototype delivered Oct 2012 as scheduled, 3 publications; e.g. success led to the following/contracts below +successful field validation of new drug analyzer/method, achieved with rehab center patient saliva samples ∗ NSF Harvard Med detect drugs blood/urine/saliva, consortium-PI, our SER-methods/purchase analyzer ∗ NIH SDS drugs as coPI/PM; Yale Med Psychiatry for clinical studies saliva, success cocaine/diazepam

∗ developed SERS to measure pesticides, toxic chemicals on and in food USDA, as CI/PM, SER-detection of pesticides on and in fruit/vegetable matrices, parallel study std GC method performed exhaustive study pure actives and formulations, degradation/stability, residue analysis

∗ developed SERS - dye analysis paintings Metro Museum Art NY / exhaled breath condensates Miami Med

Results 33 papers 3 chapters 71 presentations, 4 patents, completed 45 programs/contracts $18 million revenue Sr. Scientist / R&D Program Project Manager Real-Time Analyzers Middletown CT, 2003-2006 As a Senior Research Scientist/Chemist, recruited by RTA to Lead and Manage R&D Programs and Projects involving Raman-SERS Applications, New Product and Detection Platform Development

1st yr demonstrated experimental expertise, technical/managerial ability, could take charge, immediate effective control of challenging project (make mine), multiple projects involving new concepts (multitask, adapt master) e.g. jump-started 3 stalled R&D projects1st yr; creative thinking – innovative solutions overcame challenges

∗ as PM, managed daily operations, coordinated all activities of R&D team, updated stakeholders ∗ engaged clients/monitors to define clear priorities for success: identify constraints/drivers/degrees of freedom w/r to project cost, schedule, quality, staffing, functionality features; flexibility diagram used to visualize choices ∗ planned R&D projects, set activities, timelines, milestones for project completion - redefine conditions if changed ∗ documented work/changes implemented (why/result); continuous review process - results / make adjustments ∗ led team of chemist/chem.eng/research scientist/ application, product development, software specialist ∗ analyzed data, wrote technical reports, presented progress results, led team in weekly performance meetings ∗ in program manager role also responsible/accountable for coordinated Mgmt of multiple projects, made certain projects align with company goals, to maximize benefits/minimize risk + ensured quality of project deliverables

∗ as a senior research scientist/chemist, conceived planned organized complex highly original scientific research

∗ compiled required science technical sources, analyze, direct integration of info into decision process ∗ provided technical expertise, proposals, publication of scientific research, professional presentations ∗ applied knowledge and skills as an accomplished synthetic and experimental research chemist in the lab e.g. organic/inorganic synthesis; new ligands, transition-metal/organometallics; - precursors/catalytic systems e.g. expanded to nanomaterials/nanoparticles/nanocomposites/polymer-hybrids/sol-gels; - sensors/separation ∗ enforced quality and safety; ensure equipment calibrated, labs up-to-date/compliant + staff properly trained e.g. understand familiar with regulatory requirements/compliance/quality systems e.g. FDA, GMP GLP; QA CDC/NIH biosafety & EPA/OSHA procedures, prepared for IRBs e.g. clinical/human subject trials/samples maintained all records/documentation; MSDS, SOW, authored/followed SOPs e.g. method validation

pushed company technology forward in trace-chemical detection, nanotechnology; sampling/sensing/analyzing ∗ developed unique nanomaterials, SER-active Ag/Au silicon-alkoxide based sol-gel chemistries / in 6 domains 1st NASA P1/P2 as PI, capillary/chip to detect biomarkers indicating muscle bone-loss / renal stone-formation, + drugs metabolites used to counter adverse effects; establish calibration curves/analytical method validation extracted identified from urine at required ng/mL mg/L levels under 10-min, for smart toilet ISIS space station 2nd NASA P1/P2, +P3 as SI/CI with ASTID-JPL micro structured sol-gel & PMMA patterned chips / lab-on-chips targeting biologicals/ biochemical signatures of life on surfaces/soils/liquids; LOCs for future Mars mission 3rd EPA P1/P2, +P3 as SI/CI, NeSSI application with Parker-Hannifin, modular miniaturized automated sampling manifold for online process chemical analysis; condition/regulate sample stream into SER-capillary cartridge targeting chemical agents pesticides TICs PAHs explosives at ppb in water, for NY Kensico Resevoir; CPAC 4th DoD P2 as SI/CI, Edgewood facilities, detect chemical warfare agents/hydrolysis products, water/surface/air plus characterization of oligonucleotide series/perturbations, relevant to anthrax, with Prof. Elliot UT Austin 5th NIH NSF as SI, with cancer research group Yale Med, detect chemotherapy and prescription drugs in saliva 6th USDA as SI, rapid field analysis, detect pesticide residues on food feed and baby formula at tolerance levels

∗ new chemistries/substrates superior to prior RTA SER-products; detect more chemicals/lower levels1ppm-1ppb e.g. microplates 96/384 glass BK7/quartz plastic polypropylene/polystyrene well bottoms; small fixed-vol sample capillaries variable volume flow, small microL to large mL samples, selectively extract and concentrate e.g. functionalized by incorporating different polar groups/Si-alkoxides, and hydrophobic/hydrophilic polymers e.g. optimized reaction, conditions, catalyst; via combinatorial chemistry, high-throughput screening (HTS) ∗ produced novel chemical means to modify surface morphology, particle size, during metal reduction process ∗ developed new sol-gel processing methods, in-situ and ex-situ; preparation/reaction/curing/post-treatment steps ∗ developed new coating methods (spin/dip/spray/flow) on glass/plastic vials/disks/plates/tubes/capillaries, new coating formulations (sol-gels/nanoparticles/polymers) e.g. spin-coated vials/disks, open tubular capillaries ∗ polymer modified Ag sol-gel, minimized cracks with cure+detachment after reduced; made plates viable product e.g. later partnered with DigiLab, our plates in their compact 785/532 Raman plate-reader systems (see paper) e.g. later with new method to seal vacuum pack, see various groups purchase multiple plates, repeat orders ∗ enhanced developed new applications by coupling new SERS products with existing upfront technology e.g. pre-treat extract separate concentrate via SPE IEX LLE, provides pristine sample to sol-gel sensor e.g. capillaries combined in-line, flow through pre-treat capillary directly into SERS sol-gel capillary sensor e.g. used ion-retardation resins, micro-filters, syringe or peristaltic pumps for controlled flowing e.g. conix plate-reader, pre-treat columns fixed above plate simultaneous collect aliquots in wells/measure SER ∗ developed proprietary reagents for releasing anthrax spore and bacterial spectral signatures (new patent) ∗ used thermal decomposition, ultrasound activation, photolysis, microwave; alternate synthesis of Au/Ag NPs

contributed in application / product development of Raman spectroscopy / new robust Raman analyzers ∗ tested new spectrometers; showed analytical, suitable for on-demand or continuous process monitoring e.g. on-demand fuel monitoring, buildup model +additives; see validation, fluorescence minimal at1064nm e.g. analytical tool to monitor control chemistry in small scale reactors (microL-mL) esterfication/drug synthesis e.g. in support of PAT, improve by using analytical NeSSI sampling and input systems coupled to microreactors later develop feedback controls, monitor biomass conversions; catalyst/enzyme; biofuel synthesis; UCONN

engaged in continuous professional self-development plan to supplement or expand core knowledge ∗ confident - self-driven, can master new difficult concepts, critically assess potential + risk; utilize e-Learning example set -where acquired expertise experience later benefited company; new capabilities/opportunities ∗ proper handling/culturing/purifying BSL-2,3 pathogens at URI, web-seminars/on-line at Yale e.g. motivated new SERS sensors/bioassay immunoassay development, functionalize for specificity e.g. biopan phage-display Lib/ID binding sequence; peptide synthesis/FMOC/add protected cys to C-term ∗ biofluid properties; storage handling characterization (saliva urine mucus plasma), on-line/papers e.g. motivated pharmacokinetics/stability of biomarkers, drugs, metabolites in biofluids; on-line/NIH/papers e.g. obtaining storing measuring clinical specimens (NIH protocols, Internal Review Board) ∗ new pathogen/drug capabilities later provided over $3 million additional government/private contracts +patents and new contracts with new collaborations expanded our technology, product pipeline, and public recognition

applied statistical analysis/validation methods as performance tools for R&D products/processes ∗ used analytical figures of merit (FOM), to routinely define or optimize system/method performance e.g. screened specific analytes on SERS-substrates; accuracy precision selectivity sensitivity LOD/range standard performance measures for analytical method development and validation ∗ developed receiver operating characteristic (ROC) curve analysis with ECBC to evaluate SERS substrates e.g. best tool; define - reproducibility specificity sensitivity confidence, to select optimal drug and bioassays ∗ utilized statistical design of experiments, advanced Six Sigma method, to screen factors (inputs)/effects on output (responses, performance measures)/establish maintain QC/optimize process/design robust product e.g. achieved significant improvements in capillary sensor quality & sol-gel process efficiency ∗ used principle component of analysis, combine with DOE (OVAT)/or just DOE multivariable factorial method

applied project management techniques (PMI, best practices, principles, tools) to R&D projects programs ∗ used PM-method software (initiate/plan/execute/control/close); tools to measure progress/track task ∗ e.g. M123-PMM, Clarizen (SAAS on-line), Microsoft suite (Office, Excel, PowerPoint, Word, Project) ∗ towards performance Mgmt used standard R&D measures adopted in industry and government, e.g. tracked Earned Value metrics -schedule variance, cost variance, schedule performance index, cost performance index forecasted -estimate at completion/estimate to completion; other -variance at completion/budget at completion ∗ implemented continuous risk assessment; project selection/evaluation prioritization, terminate if required e.g. in-house project of undeniable potential/detecting explosives by SERS, was unable to surpass nominal performance of current technology (or prior results of supplemental work initiated in a funded EPA project); made decision - no improvement over initial results, terminate rather than consume anymore time / RTA-funds complications: pressure to apply more resources/persistent contractual request to develop explosive analyzers

promoted successful project completions by developing and using standard templates/tools/methods ∗ created project schedules, cost summaries, milestone charts, other key management reporting tools; include provisions for alternate vendors/suppliers, +organize/obtain/schedule - resources/materials/services upfront ∗ e.g. PM Methodology123, all templates for initiation planning execution closure, can modify each as needed ∗ to further ensure success, managed tasks daily; 1st review project schedule, 2nd have 15min informal kickoff meeting, 3rd do walkabouts and inspect, be visible and energetic not fixed behind desk, 4th review team timesheets/ time spent on projects; applied a more informal Mgmt approach, most effective for this team ∗ prioritized work for team, go over tasks assignments, bring them in-line with big picture, so not overwhelmed

improved project performance by creating organizing maintaining historical information/data on all projects ∗ tracked key performance measures during life of project, collect most of required information by completion e.g. quantitative costs/schedule/work effort/scope/quality; qualitative variances/risks/organization resources ∗ for completed projects, final values of measures sufficient; BUT found best practice is collect measures at interim points during project life cycle; invaluable in identifying where problems occurred for off-track projects e.g. made standard practice of analyzing evaluating data compiled daily for each project on a weekly basis ∗ prepared agenda and held project reviews before formal reports due (monthly quarterly semiannual basis) provided effective means to capture crucial feedback while fresh in each team members mind

∗ after every project took time to summarize important observations, measures, best practices to implement ∗ lessons learned – acknowledged mistakes, analyzed causes, identified means to prevent in future projects ∗ based on historical similarities (scope, tasks, resources, cost profiles, potential risks), reused well-designed docs created for past projects as time-saving templates; develop better estimates based on past experience ∗ by analogy implemented common tasks in R&D projects, general form/structure, into most research proposals ∗ embraced external project reviews via presentations (to monitors) monthly/quarterly; confirms on-track or not

developed PM control system to deliver on expectations; improve cost, time, work effort, duration estimates ∗ use task-based approach; WBD -break R&D project into small components, estimate each task separately ∗ e.g. time: bottom-up, consider each task incrementally-add; top-down, overview past timeline/+tasks by analogy ∗ e.g. in our std R&D proposal format, used range estimates in schedule for each task/associated milestones ∗ e.g. time-box, estimate fixed schedule, force completion by deadline, focused on cost/scope so date met ∗ combined multiple project estimation techniques for best results, found no single approach to suit all projects

incorporated Total Quality Management (TQM) tools/templates involving continuous improvement (CI) ∗ utilized quality performance and problem solving tools / Quality Control tools; with PM methodology ∗ e.g. cause-effect diagram/flowchart/histogram/scatter diagram/pareto chart/control chart/checklist ∗ to implement CI applied PSDA cycle, benchmarking, root-cause-analysis for problem solving ∗ e.g. ID problem, root causes, determine alternatives/impacts, select best alternate, resolve problem and validate ∗ familiar with ISO 9001 standard for QMS, RC 14001 respond care technical std; also ASTM/NIST/MIL-STD

combined techniques to develop project methodology that works best for RTA culture/environment/strategy ∗ ID specific PM issues/review organization factors/define scope plans phases, WBS; risk/change Mgmt; planning ∗ e.g. rigorous PM problematic, best suited for mfg where innovation is incremental vs. potential break-through or disruptive innovation and inherent uncertainty/variance in novel R&D; accepted PM limits/adapt modify for R&D ∗ analyzed tools techniques to capture map our baseline; current and best practices, develop process map ∗ exploited best practices and standards; develop and validate process improvements ∗ created tools/templates/forms that facilitated use, ensured consistency, allowed appropriate flexibility creativity

integrated Idea and Innovative Management methods for application to R&D and Innovative projects, as well ∗ applied to PM/Leadership for more controlled sustainable approach ∗ plus create environment that fosters sustainable measurable innovation in new product development (NPD)

Results, 6 papers 31 presentations 9 projects/programs (initiated/awarded NASA R&D contract as PI, 1st yr) Research Associate (Post-Doctorate) University of Arizona, Tucson AZ, 2000-2003 Recruited as a Research Scientist/Chemist for Post-Doctorate, by world-renowned synthetic chemist, enzymologist spectroscopist Prof. J.H. Enemark; to take charge improve bio/clinical specimen management of valuable enzyme/protein samples (human/animal/bacterial sources) AND conduct NIH independent research in design synthesis characterization of high-valent transition-metal catalytic organometallic systems / biomimetics

developed new synthetic routes and complexes, demonstrating innovation/creativity; see big picture/best path ∗ synthesized complicated organic ligands, inorganic, organometallic systems; bioorganic/small molecule models ∗ used Schlenk/glove-box methods excluding ppb H2O/O2 in synthesis purification air/moisture-sensitive materials ∗ utilized automation robotics, dispensers, parallel methods to perform experiment sets in less time when possible ∗ designed optimized realized new pathways/precursors towards biological relevant small molecules and catalysis e.g. Mo/W oxo/sulfido/NO dithiolene/dithiolate and thiol systems; different oxidation states, coordination spheres e.g. isolated {O=M(S-S)} moiety with tripodal TPz-borate ligand; quaternary-salts/cyclopentadienyl complexes e.g. advanced purification method development; ensure sample integrity, provide quality single crystals for XRD ∗ defined org/inorganic/organometallic reaction mechanisms; isolate synthesize characterize intermediates ∗ e.g. new and effective catalytic routes/precursors towards obtaining target compounds of industrial importance

established and managed equipment/instrumentation for general chemical studies ∗ employed analytical techniques, wide range data analysis, characterization tools (e.g. IR, MS, NMR, CV) ∗ modified rebuilt synthetic/spectroscopy labs (UV/vis/NIR, FT-IR; Schlenk lines, pumps, hoods, stills, waste)

developed applied high-level computational platforms to correlate theory with experimental results ∗ used Gaussian/ADF computations to evaluate spectroscopic results, model geometric electronic structure mentored coached members in research group towards providing positive contribution to programs ∗ provided clear vision long-term goals/3 yr plan to graduate students; led directed their research, success

coordinated equipment use for biological studies e.g. enzyme purification / sample preparation ∗ maintained GC/FPLC/HPLC; chromatography, centrifuges, assay and pure-water equipment in 3°C cold room e.g. 2 week nonstop method, 9mg active sulfite oxidase collected isolated purified from 20kg chicken liver ∗ managed biospecimen inventory (old and new samples), cryogenic storage, biosample integrity, tracking

very productive output, made significant contributions to field, recognition - important high-caliber research ∗ Raman rR, XRD, PES, EPR, UVvisNIR, MCD; probed redox/geometry change w/r to electronic structure ∗ Results provided spectroscopic derived active-site mechanisms, new syntheses, 6 papers, 3 abs, 5 prof. talks includes invited papers e.g. with National Academy of Sciences; JACS paper with Northwest National Lab

OTHER EXPERIENCE

Ph.D. Research University of New Mexico, Albuquerque NM Recruited as a Experimental Research Chemist / Spectroscopist for Ph.D., by new assistant Prof. M L. Kirk; Developed conducted research, designed managed equipment instrumentation funded by NSF, Sandia National Labs, Petroleum Research Fund, in syntheses and spectroscopic characterization of various small molecule organometallic precursors, catalytic models, of e.g. molybdenum / tungsten pyranopterin enzyme active sites

applied expertise in analytical/physical/organic/inorganic chemistry, biochemistry, instrumental analysis ∗ designed constructed fully functional analytical synthetic lab from scratch; Schlenk lines, glove boxes ∗ major role design/construction state-of-art spectroscopy lab(VT-Raman/rR IR VT-UVvisNIR VT/VH-MCD) e.g. Ar, Kr, Ti:sapphire tunable ring dye, HeNe, CW/pulsed lasers, optics, cryostat, spectrometer, CCD e.g. CD, polarization optics, Oxford 7T magnet (VT/VH) cooled optical cryostat pumped 1.85K LHe, PMT e.g. UVvisNIR absorption 185-3200nm, with VT-Jasco optical cryostat LN2 77K / LHe 4K, in custom cradle

applied programming knowledge experience for developing new software analysis and enabling tools ∗ developed programs, e.g. performing normal coordinate analysis, Fortran code compiled PC/MAC formats developed novel combined multidisciplinary approach for probing small molecule electronic structure ∗ rR profiles provided mechanistic insight how enzyme regenerated after catalysis “Oxo-Gate-Hypothesis” ∗ Results help provide funding, tenure for new Prof.; 3 publications, 2 abs, 3 prof. presentations at ACS/Gordon instructed organic & inorganic labs in advanced synthetic/characterization methods as part of DOE fellowship Education Post-doctorate organic/inorganic/organometallic synthetic chemistry, catalytic systems, enzymology, UAZ PhD in Chemistry, at University of New Mexico, Albuquerque NM for M.S. fulfilled requirements in both Physical and Inorganic Chemistry, UNM for B.S. fulfilled Chemistry major requirements, minor Applied Mathematics/Computer Science, UNM B.A. in Biology / Biological Sciences (minor Organic/Biochemistry) Austin College, Sherman TX

ADDENDUM - PROFESSIONAL EDUCATION / DEVELOPMENT - FRANK E. INSCORE ADDENDUM 1

EDUCATION Post Doctorate Materials Science Bioorganic-inorganic / Organometallic chemistry University Arizona Tucson AZ Postdoc, advanced in synthesis, extraction, separation, purification, characterization and modeling of new molecules & materials; expert in applying combined multidisciplinary theoretical & spectroscopic approach Doctor of Philosophy (Ph.D.) in Chemistry, University of New Mexico, Albuquerque NM PhD awarded / MS fulfilled, in both Physical Chemistry & Inorganic Chemistry (analytical chemistry minor) Bachelor’s degree, Biology / Biological Sciences (organic / biochemistry minor) Austin College Sherman TX BS & BA include strong background in multistep organic / inorganic synthetic chemistry & characterization, applied mathematics, quantum chemistry & physics, computer science & programming, behavioral science PROFESSIONAL AWARDS, HONORS & RECOGNITION NSF+ Frost & Sullivan awards for developing & commercializing new nanotechnology, sensors & materials NASA Innovative Research Award in Medical Technology for SERS, lab-on-chip with Jet Propulsion Labs Chemistry Dept. Travel awards to ACS & Gordon Conferences, and 2 PhD Research Excellence awards Prestigious DOE GANN Fellowship awarded as entering graduate student in Dept. Chemistry PhD program Dean Uhl Award for Excellence in Chemistry Inducted Kappa Mu Epsilon National Mathematics Honorary Presidents Honor Roll; Golden Key National Honor Society, Biology award, National Merit Scholar PROJECTS, PUBLICATIONS, PATENTS & PRESENTATIONS (see full description / lists in support appendices) 15 proposals awarded as Principle Investigator (Principle Scientist), 3 as co-PI, 6 as SI (Senior Scientist), 50 projects programs completed on-time and within budget, providing over $15,000,000 in revenue to RTA 51 peer-reviewed publications, 4 patents, 50 presentations PROFESSIONAL AFFILIATIONS Sigma-Aldrich Global Advisors, Society Applied Spectroscopy, Coblentz Society, UAZ and UNM Alumni TRAINING & SELF-DEVELOPMENT (includes online tutorials, webinars, eBooks - documents) Performed core competency 360° self-assessment, expanding program project management methodology / software capabilities to meet R&D challenges + future needs for changing global economy / cultural diversity and in preparation of PM certification as consultant 2013-2014; 1st-aid CPR RC-certified; BSL1-3 and ATCC-BEI trained at URI & Yale online; protein peptide synthesis online at NEP; multivariate chemometrics webinars CAMO; chemical safety training instruction @UNM in Hazardous Material Handling (EPA), First Responder Procedures (ER, Fire Dept), Laboratory Safety (Chem. Dept), Laser Safety (Los Alamos National Lab); Patent writing, market analysis, product commercialization @NSF-DCN 2009; SEM, patterned microchip fabrication @AFR 2004-2006 PROFESSIONAL GLOBAL NETWORKS / WEBSITES / URLS / CONTACTS LinkedIn: 9 professional recommendations, 450+ peer endorsements across 50 expertise & skill areas, 1500+ networking members/contacts, profile top 1% viewed 2012/featured 2013; www.linkedin.com/in/finscore2014 see additional sites - http://frank-inscore.branded.me, www.slideshare.net/inscore - feinscore@gmail.com

ADDENDUM 2

LinkedIn 50 Groups (representative groups / associations participate in): Analytical Chemistry, Applied Multivariate Analysis, CAMO, Sensors, Catalysis, Center Biosensors Biochips, Chemistry World, Combinatorial Chemistry, Medicinal Chemistry, Drug Discovery, Insight Pharma Reports, Homeland Security, Los Alamos National Laboratory, Molecular Spectroscopy, Nanotechnologists Nanotech Pesticide Analysis, Professional Chemists, Raman, Spectroscopy, Spectroscopy, Surface-enhanced Raman Open Photonics, Quality Regulatory Guidelines, Chemical Professionals Network, Raman Research Institute

LinkedIn 50 Skills (with 450 peer endorsements of): spectroscopy, chemistry, analytical chemistry, nanotechnology, organic chemistry, characterization, nanomaterials, Raman, chromatography, biochemistry, UV/Vis, materials science, nanoparticles, NMR, inorganic chemistry, laboratory, science, scientific writing, physical chemistry, electrochemistry, assay development, R&D, research, data analysis, biotechnology, molecular biology, SERS, catalysis, HPLC, sensors, powder X-ray diffraction, purification, IR, surface chemistry, gas chromatography, FTIR, mass spectrometry, biosensors, sol-gel, lifesciences, optics, physics, formulation, design of experiments, experimentation, organic synthesis, fluorescence, chemometrics, computational chemistry, organometallic chemistry

ADDENDUM 3

OTHER SKILLS and TRAINING from COURSES

FOR GRADUATE / MS DEGREE: ∗ Chemistry - Advanced Quantum Physics, Quantum Mechanics 1-2, Molecular Quantum Chemistry, Computational Chemistry, Statistical Thermodynamics, Physical Methods for Chemists 1-2, Spectroscopy and bonding, Bioinorganic Chemistry, Advanced Organic Chemistry and Mechanisms, Research1-4

FOR UNDERGRADUATE / BS and BA DEGREES: ∗ Chemistry - Physical Chemistry 1-2 +Lab 1-2, Group Theory, Inorganic Chemistry, Adv Inorganic synthesis and characterization +Lab, Quantitative Analytical Chemistry +Lab, Instrumental Analysis +Lab, Biochemistry +Lab, Spectroscopic Methods for Characterizing Organic Compounds, Organic Chemistry1-2 +Lab 1-2, General Chemistry 1-2 +Lab 1-2 ∗ Applied Math - Complex variable Analysis; Advanced Partial Differential Equations, Applications of Partial Differential Equations, Ordinary Differential Equations, Advanced Vector Analysis, Combinatorial mathematics, Discrete Mathematics, Linear Algebra, Calculus 1-4, Trigonometry, Plane geometry, Advanced college algebra, Advanced Elements of Logic 1-2, General accounting, Statistics and business ∗ Computer Science - Fortran for engineers, Abstract Data Programming 1-2, Computer science 1-2 ∗ Physics -Advanced engineering mathematics, Mathematical methods for physicist, University physics1-2 +Lab 1-2, General Physics +Lab ∗ Biology - Advanced Systems Physiology +Lab, Anatomy-Physiology 1-2 +Lab 1-2, Developmental biology +Lab, Molecular Biology +Lab, Genetics +Lab, Cell Biology, Immunobiology +Lab, Microbiology +Lab, Advanced Invertebrate Zoology, Comparative Vertebrae Analysis +Lab, Neurochemistry and psychopharmacology, Pathophysiology, Biology 1-2 +Lab 1-2, General Botany +Lab ∗ Behavior - Cognition and Perception, Psychology 1-2, Sociology, Bioethics in Medicine

FOR UNDERGRADUATE / RESEARCH INTERNSHIPS: ∗ Clinical studies (drug test, ELISA assays) of emphysema patients UT Health Center Dr. Cohen MD PhD ∗ Retinal/vision studies at Southern Methodist University (advanced systems physiology) ∗ Hormonal studies and behavioral effects at Austin College (advanced physiology, applied medicine) ∗ Recombinant molecular biology studies at Austin College (genetics) ∗ Spectroscopic characterization of organic compounds Austin College (organic chemistry)

ADDENDUM 3 continued, OTHER SKILLS / KNOWLEDGE AREAS / TECHNICAL / COMPETENCIES CORE COMPETENCIES, SKILLS & KNOWLEDGE AREAS Fortran/C++, Mathmatica, Unix, Microsoft Office Suite, NSI LabView, CAMO Unscrambler, PCA PLS SIMCA DA chemometrics, design of experiments, statistical analysis, ROC curve analysis, Six-Sigma methods RCA DOE CI; Cmap, Smartdraw, Gaussian, Clarizen, MPMM123, Stage-gate, Tableau visual analytics, Chemdraw Pro, Wordle, multistep synthesis, combinatorial chemistry, chemical physical properties; industrial monitoring, proposal writing, nanotechnology, biotech, nanomaterials, department manager, research and development, product development, product formulation, cost reductions analysis, team management building, quality manager, QA/QC/TQM, design, process product improvements, hazardous chemical waste, GMP cGMP GLP FDA OSHA EPA CDC regulations, internal review board, ICH, lab safety, technical services, finance, production, ISO, internal rollout, cost out, SOP NDA, documentation, management tools templates, PAT, NeSSI; spectroscopy, electrochemistry, crystallography OTHER KEY GENERAL MANAGEMENT and TECHNICAL COMPETENCIES Technical report writing, Presentations, Grants, Proposals, Contracts, Patents, Product improvements, Process improvements, Program management, Project management, R&D Director, Manager, Research, Development, Execution, Data analysis, Data management, Biospecimen management, Analytical Mgmt, Analytical methods, Analytical Lab operations, NIH clinical sample human subject protocols, Internal review board (IRB), regulatory compliance, OSHA, Project management principles Budget planning, Strategic planning, Support marketing sales develop client relations collaborations industry govt. academia

Chemistry, Biochemistry, Organic chemistry, Inorganic chemistry, Physical chemistry, Analytical chemistry, Sol-gels, Surface chemistry, Materials science, Nanotechnology, Nanomaterials, Catalysis, Synthetic chemistry, Purification, Chromatography (flash, SE IEX), Extraction (LLE SPE Soxhlet), Fuel analysis

Statistical design of experiments (DOE), Chemometrics, Multivariate analysis, High throughput screening, Combinatorial Parallel synthesis, Computational Theoretical chemistry (Gaussian, ADF, DFT), Informatics, Chemical databases, Molecular modeling, Spectral libraries, Process analytical technology (PAT), Chemical and Physical properties, Instrument calibration, Method development, Verification and Validation, Analytical Figures of Merit (FOM), Receiver Operating Characteristic (ROC) curve analysis

Life sciences, Healthcare science, Medical devices, Biosensors, Biology, Molecular biology, Pathogens, Biological warfare agents, Biofluids, Biomarkers, Nucleic acids, Peptides, Proteins, Enzymes, Antibodies, Oligonucleotides, Biochemicals, Toxins, Biotech, Chemical Industry, Defense, Environment, Food and beverage, Chemical agents, Pesticides, Toxic industrial chemicals, Pharma R&D drugs, Pharmaceuticals, Pharmacokinetics, Drugs, Metabolites, Illicit abused drugs, Assay development, Clean room, Biofuels

Spectroscopy, Characterization, Raman, resonance Raman, SERS, SERRS, IR, FT-IR, UV/Vis-NIR, MIR, UPS/XPS, XAS/EXAFS, XRD, XRF, TEM, SEM, EDS, AFM, NMR, EPR, MCD, CD, CV, CE, LC, GC, MS, MS/MS, HPLC, FPLC, FID, ICP, AA FAA, BET, TGA/DSC, Fluorescence, Bomb calorimeter

Independently initiated, formulated, wrote, won following 15 SBIR SERS proposals contracts as PI (Principle Investigator) in addition to managing and performing key experiments for each; as PI/PD wrote interim and final reports:

A Label-Free SERS Capture Assay in Microchips for Biological Warfare Agents This NSF SBIR program (through Phase III) is developing an innovative surface-enhanced Raman spectroscopy (SERS)-capture assay that can detect, identify and quantify the presence of Category A (or B) spores, pathogens and bio-toxins in aerosols at required sensitivity (104 spores/m3, 100 organisms/ m3, 2-300 mg toxin/m3) within 10 minutes. Role: PI NSF Phase I IIP-0810335 Inscore (PI) 07/01/08-12/31/08 $100,000 NSF Phase II IIP-0956170 Inscore (PI) 04/01/10-03/31/12 $650,000 NSF Phase IIB IIP-0956170B Inscore (PI) 04/01/12-03/31/13 $250,000 total $1million

In Phase I performed measurements and developed method for peptide-functionalization of silver sol-gel and subsequent protocols for SERS-capture Bacillus cereus spore assay. Feasibility for capture and discrimination was demonstrated by SERS. In Phase II, developed-tested modified filtered microplate that can detect, identify and quantify presence of Bacillus anthracis-sterne spores in aerosols at required sensitivity 104 within 10 min. PI, by his own initiative, obtained several private contracts based on this work, which was matched and awarded by NSF in Phase IIB, allowing assay validation with real bioagents at URI/ECBC (BA-ames, Yersinia pestis, Francisella tularensis, Clostridium botulinum A, Ricin and SEB). A Chip-Scale Sensor for High-throughput Detection of Multiple Waterborne Pathogens This DARPA SBIR program (through Phase III) is developing a chip-scale SERS-based assay that can detect, identify, and quantify the presence of Category A (or B) bioagents in water at required sensitivity (e.g. 400 B. anthracis spores per liter water) within 10 minutes. Role: PI DARPA Phase I N10PC20077 Inscore (PI) 02/08/10-08/08/10 $100,000 DARPA Phase II N11PC20171 Inscore (PI) 06/30/11-06/29/13 $750,000 total $850k

In Phase I performed measurements and further developed the method and chemistry for peptide-functionalization of silver sol-gel and subsequent protocols for the SERS-capture Bacillus anthracis (Sterne) spore assay: capillary assay stable for 6-mo and could detect-discriminate single-digit BA endospores within 10-min. In Phase II, modified-optimized chemistry and conditions to minimize non-specificity, and designed multiplexed-assay on chip/cartridge and measure 103 bacteria/mL SERS Food-Borne Pathogen Analyzer This program contract for Kraft Foods developed a novel food analyzer for measuring discriminating pathogens at genera level, Listeria and Salmonella at 1-10 cells, with no culture pre-enrichment from food matrices within 8-hrs. Role: PI Kraft Foods 580008 Inscore (PI) 11/01/10-03/31/11 $60,000 total $60k

Applied assay protocols/ peptide-functionalization methods developed in NSF above to food-borne pathogens. Directed assay development incorporating antibodies as alternative molecular recognition elements (MRE’s) for functionalizing the SER-active sol-gels in this program. These AB assays (L. monocytogenes, S. typhimurium, E. coli O157: H7) were found to be more conducive for selectively detecting whole bacterial cells, and were applied to the DARPA program as a result. Saliva SERS Drug Detector This three year program contract with the United Kingdom’s Home Office developed a surface-enhanced Raman spectroscopy (SERS) based roadside screening device for detection of drugs (RSDD). The proposed SERS-RSDD can extract, identify, and quantify the presence of drugs (and metabolites) in driver saliva at the requisite 10-8M within 8 minutes. The SERS-RSDD consist of a saliva sample collector, plastic syringe containing a reagent, a SERS-based lab-on-a-chip (LOC), and a field-usable Raman analyzer. Role: PI UK Phase I 580004 Inscore (PI) 09/01/09 -02/28/10 $100,000 UK Phase II 580005 Inscore (PI) 04/07/10 -03/30/11 $750,000 UK Phase III 580010 Inscore (PI) 04/01/11 -09/30/12 $350,000 total $1.2million

Performed necessary measurements to demonstrate feasibility in Phase I; key contribution in developing extraction method and testing saliva analysis in Phase II as well as expanding SERS Library to over 300 drugs; further directed the design and testing of saliva analyzer in Phase III, which was delivered to UK on schedule.

Embryo Viability This program (subcontract) for Viagen’s NSF Phase I-II SBIR developed a rapid, non-invasive method for bovine embryo viability assessment using SERS. Role: PI Viagen NSF Phase I subcontract Inscore (PI) 07/01/11-09/01/11 $20,000 Viagen NSF Phase II subcontract Inscore (PI) 2012-2013 $200,000 total $220k

Performed necessary measurements/ chemometric analysis to demonstrate feasibility in Phase I, wrote Phase II subcontract.

feinscore@gmail.com

1. A1.1

Appendix 1: Research Projects & Awarded Support at RTA

Rapid Identification of Blood, Urine, Saliva Toxins & Bacterial Infections with SERS/LSS Flexible System This 4 year program (subcontract) for NSF with Harvard Medical School, Prof. L.T. Perelman (at Beth Israel Deaconess Medical Center) will measure toxic substances in saliva, concurrent with measurements in blood plasma and urine, which will provide valuable information to the ambulance, emergency room, and hospital medical staff. Role: consortium PI Harvard NSF 4 year subcontract Inscore (consortium PI) 09/01/12-08/31/16 $280,000 total $280k

Independently performed feasibility measurements, wrote RTA’s contribution to proposal and obtained subcontract. SERS-ID Instrument for Trace chemical Analysis This 6-month private development contract provided the Swedish DoD Research Division with a SERS-based Raman analyzer for detecting drugs, chemical agents, and explosives. Role: PI Swedish DoD contract/purchase Inscore (PI) 07/01/11-12/21/11 $100,000 total $100k

Independently pursued and obtained contract for developing SERS analyzer, and 1st sale of our SERS-ID system. On-Demand Urine Analyzer This NASA SBIR program (through Phase III) developed an analyzer that can be integrated into International Space Station (ISS) toilets to measure key chemicals in urine. The analyzer employed a novel metal-doped sol-gel material to both separate these key chemicals from urine and provide surface-enhanced Raman spectra to identify and quantify these chemicals. Role: PI NASA Phase I NNC04CA50C Inscore (PI) 01/16/04-07/19/04 $100,000 NASA Phase II NNC05CA09C Inscore (PI) 11/01/04-11/01/06 $650,000 total $750k

Independently wrote and won this Phase I-II program, performed 95% of work to demonstrate feasibility (drugs biomarkers detected 10 ng/mL); deliver LOCs to selectively extract detect biomarkers and associated counter drugs in urine in 10-min. Provided significant contribution in the following 9 SERS programs by helping to initiate, formulate, write and win these SBIR proposals and contracts as the coPI/PD, and in managing and performing critical measurements for each:

A Rapid Food-borne Pathogen Analyzer This NSF SBIR program (through Phase III) will develop a Food-borne Pathogen Analyzer that can extract, detect, identify, and quantify presence of food-borne pathogens in 1-2hr at required sensitivity (1-100 cfu/g). Role: coPI/PM NSF Phase I IIP-1214986 Inscore (coPI/PD) 07/01/12-12/31/12 $150,000 NSF Phase II (awarded) $850,000 total $1million

For Phase I, feasibility will be demonstrated by developing a novel sampling system consisting of a bilayer that provides selective pathogen extraction and SERS activity to detect 103-4 cfu/g Salmonella typhimurium in spinach within 2 hours. This proposal result of resubmission and refinement of initial USDA, EPA and NSF proposals put forth. Saliva Analyzer for Diagnosing SRD Patient Drug Use This NIH SBIR program will develop a surface-enhanced Raman spectroscopy (SERS) based device for on-site analysis of drugs in saliva. This device will allow immediate assessment of both medicinal and illicit drug use by patients with substance-related disorders (SRDs), and provide medical professionals and management with quantitative data so that treatment decisions can be made. The proposed SERS Saliva Analyzer (SSA) will extract, identify and quantify the presence of drugs (and metabolites) at requisite levels in saliva (1-50 ng/mL), within 10 minutes. Role: coPI/PM NIH NIDA Phase I 1R43DA032178-01 Inscore (coPI/PD) $150,0000 7/01/12-12/31/12 NIH Phase II (awarded) $850,000 total $1million

This Phase I program will demonstrate feasibility by detecting two priority drugs (cocaine and diazepam) in saliva at physiologically relevant concentrations and analysis time. This program was taken directly from UK to continue R&D. Analysis of Biofilms, Bacteria in Oral Cavity 3-phase contract in 2010 plus instrument sale in 2011 and continued support for analyzing biofilms/bacteria/proprietary agents in oral cavity by SERS. Role: coPI/PM Colgate-Palmolive contract Phase I-III 2010-2012 (support continues) $150,000 total $250k

Performed initial feasibility studies, and continues to manage this program. Field-Portable Pesticide Residue Analyzer The goal of this program was to develop a field-usable analyzer capable of measuring pesticide residues on or in food in 5-minutes or less. Role: coPI/PM USDA/ Phase I 2006-33610-16834 05/01/06 - 12/31/06 $100,000 total $100k Developed new gold substrate for flowing larger sample volume to extract pre-concentrate analytes and improve sensitivity.

feinscore@gmail.com

2. A1.2

Research Projects continued

Multiplexed Chemical Sensor for Water Security Phase I-III contracts provided EPA with chemical sensors that can be multiplexed into water distribution systems to provide early warning of poisoned water supplies (chemical warfare agents, pesticides, toxic industrial chemicals) Role: coPI/PM EPA/ Phase I EP-D-05-034 03/01/05-08/31/05 $100,000 EPA/ Phase II EP-D-06-084 05/01/06-04/30/08 $400,000 EPA/ Phase IIB EP-D-06-084B 04/30/08-05/01/09 $250,000 total $750k

Developed chemical sol-gel sensors that can be multiplexed into water distribution systems to provide early warning of poisoned water supplies at ng/mL, developed Search and ID algorithms and libraries, and with assistance, helped to partner with Parker-Hannifen, using their IntraFlow system (NeSSi application). Wrote all technical interim reports, and contributed significantly to the final reports. PhaseIIB awarded for validating system at NY Kensico Resevoir. Provided a significant contribution to the following 8 SERS programs as PM/CI, performing measurements and experiments critical to the success of each, and to writing the final technical reports.

Lab-on-a-chip Astrobiology Analyzer The goal of this program is to develop a surface-enhanced Raman (SER) sensor capable of detecting and identifying amino acids and other bio-chemicals in exobiology applications. Role: CI NASA/ARC Phase I NNA04CB35C 01/16/04-07/15/04 $100,000 NASA/ARC Phase II NNA05CP04C 11/23/04-11/23/06 $600,000 ASTID/JPL Phase III, 1280799 2007-2010. Role: CI; Dr. E. Wong (PI) JPL, to design and test LOC’s. $400,000 total $1.1million As CI/PM, performed measurements, and helped to develop method for soil analysis with LOC. Plus 3 year JPL program developed LOC’s for performing soil analysis of extracting various biological chemicals (e.g. amino acids, nucleobases, nucleosides, nucleotides, nucleic acids, peptides, proteins, DNA, RNA, polyaromatic hydrocarbons, oligonucleotides, etc.) Nanomaterial for Microchip Chemical Sensors The goal of this program was to develop a novel microchip chemical analyzer that incorporates a new nano-material that performs both separation and detection of small quantities of chemicals and biochemicals. Role: CI/SI NSF/ Phase II DMI-0215819 09/15/03-02/28/05 $600,000 total $600k

Developed new Au sol-gel chemistry in capillaries, combination of Au-Ag, as commercial SERS products to separate drugs Chemotherapy Drug Analyzer The goal of this program was to develop a microchip-based analyzer capable of providing rapid identification and quantification of chemotherapy drugs and metabolites in saliva. Role: CI/SI NIH/NCI Phase I 1R43CA94457-01 09/27/03-09/26/04 $100,000 total $100k

Developed new protocols for extracting drugs from saliva, and use of gold sol-gels, 2 patents awarded. Combinatorial Synthesis and Screening of Sensing Nanomaterial for Warfare Agents The goal of this program was to provide military personnel with a hand-held analyzer capable of detecting chemical and biological agents, as well as toxic industrial chemicals. Role: CI/SI DoD/Army Phase II DAAD13-02-C-0015 03/25/02-09/30/05 $750,000 total $750k

Developed new sol-gel chemistry in microplates and capillaries as commercial SERS products; independently investigated hydrolysis products, which led to over 10 papers. Also carried out separate programs on oligonucleotides, and more importantly developed protocols for extracting and detecting DPA from Bacilli. This also led to numerous papers and a patent. Wrote all interim reports, and contributed significantly to the final technical report. Rapid Pesticide Residue Analyzer The goal of this program was to develop a hand-held pesticide analyzer capable of identifying and quantifying pesticide residues on food or feed at tolerance levels (ppm) in five minutes or less. Role: CI/SI USDA/SBIR Phase I 2002-33619-11815 05/15/02-11/14/03 $100,000 total $100k

As PM performed measurements towards method development for pesticide in food analysis. LSPR Nanostructures for SERS to develop sensitive and reproducible SERS substrates on discs or wafers. Role: PS DoD (US Army) 05/2011-12/2011 $100,000 total $100k

Helped to supervise and manage this program, provided technical and experimental expertise, and organize final results. feinscore@gmail.com

3. A1.3

Research Projects continued

Provided a significant contribution to the following Raman programs as PM/CI, performing measurements and experiments critical to the success of each. Contributed specific sections to the final reports Analytical Control of Micro-Reactors The goal of this program was to develop Raman spectroscopy as a novel analytical tool to monitor and control chemistry in small-scale reactors (µL to mL). Role: CI NSF/ Phase I DMI-0512701 07/01/05-12/31/05 $100,000 total $100k Performed measurements, and helped to supervise and manage this program, present final results and prepare paper Anthrax Detector for Mail Sorting Systems The goal of this program was to develop a real-time anthrax Raman detector for mail sorting systems that can also identify hoax materials without interference from fluorescence. Role: CI NSF/ Phase II DMI-0349687 01/15/04-12/31/05 $500,000 total $500k Provided expertise in vibrational analysis of Bacillus and Clostridium endospores, chemical signatures and interferents High Sensitivity Raman Spectrometer The goal of this program was to design and test a hybrid Raman spectrometer suitable for 'on-demand' or continuous process monitoring. Role: CI NSF/ Phase II DMI-0296116 12/10/01-07/31/03 $100,000 total $100k Performed quantitative/statistical figures-of-merit studies as defined by the FDA Portable Raman Instrument for Fuel Characterization The goal of this program was to design and test a Raman spectrometer suitable for 'on-demand' fuel monitoring. Role: CI DoD/Navy Phase II M67854-C-5043 03/25/03-06/11/06 plus Navy contracted RTA to measure diesel samples total ~$1.6million

Provided chemistry support of synthetic fuels/additives, and helped to benchmark instrument analytical performance. Portable Fuel Analyzer (Dispersive Raman) This program will develop a rugged portable Raman analyzer that can determine 18 properties of fuels from Raman spectra. Role: PS DOD/USMC Phase II M67854-11-C-6542 07/22/11-01/24/13 total $750k Performed initial chemometric analysis on previous dispersive systems that determined feasibility of transferring models from FT-PFA; then helped confirm models working with RTAs new small portable 1064nm dispersive system Portable Fuel Analyzer (FT-Raman) Developed portable fuel analyzer (PFA) using 1064nm FT-Raman for identifying an unknown liquid/solution as a fuel or not a fuel, predicting all required critical properties, and determining if the fuel is contaminated Role: CI/SI Navy BAA contract and US Marines Phase II-III contracts 2007-present total ~$1.5million .

Responsible for developing chemometric analysis and models (SIMCA, PCA, PLS, PDA) utilizing over 1500 fuels and 500 potential contaminants; Primary focus was on kerosene based Jet fuels (e.g. JP8, JP5, JetA, JetA1, TS1, JP10) and Diesel (D1, D2, blends) collected world-wide. Also modeled synthetic (FT and Sasol) and numerous biofuels, including different blends, as well as aviation and gasoline (octane 87-123). Based on spectral measurement, Logic Tree would determine if a fuel/what fuel class (e.g. Jet or Diesel), critical and other properties (>23) and fuel type (e.g. JP5). Also tested for trace contaminants that may have effect on performance (e.g. D2 is really a B5; chlorinated solvent trace detected; contains oil). Hand-held Raman Analyzer for Chemical Identification This program is building a hand-held Raman analyzer that can perform non-contact identification of chemicals in the field. In particular to develop hand-held Raman analyzer for identifying CAIS and hazardous chemicals Role: PS DoD-CBD BAA 01/2008 DoD-Army Phase I-II W911SR-09-C-0034 05/2009-05/2011 total ~$1million

Performed measurements/analysis to help create spectral library databases on 1550/1064/976/785/633/532nm Raman systems Successful performing numerous private subcontracts e.g. Chevron-Phillips, NY Museum of Art, Miami Med School, Monsanto, over $100,000 in contracts Low Gravity Drug Stability Analyzer build a space-worthy Drug Stability Analyzer that can determine the extent of drug degradation. able to monitor the drug active pharmaceutical ingredient (API) and its degradation product concentrations as a function of time, as well as determine if a drug is suitable for use Role: CI total ~$1million NASA Phase I Nov 2012 $150,000; Phase II later awarded 2014 $850,000

feinscore@gmail.com 4. A1.4

Research Projects continued

PUBLICATIONS at RTA (2003-present) Chemical Agents/Hydrolysis 1 Farquharson, S., Gift, A., Maksymiuk, P., Inscore, F., Smith, W., Morrisey, K., Christesen, S “Chemical agent detection by surface- enhanced Raman spectroscopy” Proc. SPIE, 5269,16-22 2003 . 2 Farquharson, S., Gift, A., Maksymiuk, P., Inscore, F. “The pH dependence of methylphosphonic acid, dipicolinic acid, and cyanide by surface-enhanced Raman spectroscopy” Proc. SPIE, 5269, 117-125 2003 3 Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S “Characterization of chemical warfare G-agent hydrolysis products by surface- enhanced Raman spectroscopy” Proc. SPIE, 5585, 46-52 2004 4 Inscore, F., Farquharson, S “Detecting hydrolysis products of blister agents in water by surface-enhanced Raman spectroscopy” Proc. SPIE, 5993, 19-23 2005 . 5 Farquharson, S., Gift, A., Maksymiuk, P., Inscore, F. “Surface-enhanced Raman spectra of VX and its hydrolysis products by surface- enhanced Raman spectroscopy” Applied Spectroscopy, 59, 654-660 2005 6 S. Farquharson, F. E. Inscore and S. Christesen “Detecting chemical agents and their hydrolysis products in water” In K. Kneipp, M. Moskovits and H. Kneipp, Eds., Surface-Enhanced Raman Scattering – Physics and Applications (Springer-Verlag, Berlin) 2006 7Inscore, F., Farquharson, F. “Surface-enhanced Raman spectroscopic characterization of the chemical warfare agent vesicant HD and related mono-sulfides” in U.S. Army DAAD13-02-C-0015, Joint Service Agent Water Monitor program final report 2006 8 Farquharson, S., Inscore, F “A SERS-based analyzer for point and continuous water monitoring of chemical agents and their hydrolysis products” Internal. J. of High Speed Electronics and Systems 101-111 2007 9 Inscore, F., Farquharson, “Water security: continuous monitoring of water distribution systems for chemical agents by SERS” Proc. SPIE Optics and Photonics in Global Homeland Security III 2007 10 Huang H, C Shende, A Sengupta, F Inscore, Stuart Farquharson, “Evaluation of SERS substrates for chemical agent detection”, Proc SPIE, 8373-22 (2012) 11Christesen, S., Spencer, K., Farquharson, S., Inscore, F., Gonser, K., Guicheteau, J., “Applications of Surface-enhanced Raman detection of chemical agents in water” In: S. Farquharson, Ed. Applications of Surface- Enhanced Raman Spectroscopy (CRC Press, 2013 Chapter 2, in-press) Pesticides/Hydrolysis in water and food 1 Shende, C., Gift, A., Inscore, F., Maksymiuk, P., Farquharson, S “Inspection of pesticide residues on food by surface-enhanced Raman spectroscopy” Proc. SPIE, 5271, 28-34 2004 2 Shende, C., Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S “Analysis of pesticides on or in fruit by surface-enhanced Raman spectroscopy” Proc. SPIE, 5587 2005 . 3 Shende, C., Inscore, F., Sengupta, A., Stuart, J., and Farquharson, S. “Extraction and detection of part-per-billion Chlorpyrifos-methyl in orange juice in 12 minutes” Sensing and Instrumentation for Food Quality and Safety 4, 101–107 2010 4 Shende, C., Inscore, F., Sengupta, A., and Farquharson, S. “Surface-enhanced Raman spectroscopy: Theory and application to the analysis of Chlorpyrifos in orange juice” Applications of Vibrational Spectroscopy in Food Science, Eds. ECY Li-Chan, PR Griffiths and JM Chalmers, John Wiley & Sons, West Sussex, UK, V1, 195-209 2010 Pharmaceutics/Drugs Metabolites Biomarkers in biofluids 1 Gift, A., Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S “Five minute analysis of chemotherapy drugs and metabolites in saliva: Evaluating Dosage” Proc. SPIE, 5261, 135-141 2004 2 Farquharson S, Gift A, Shende C, Maksymiuk P, Inscore F, Murren J “Detection of 5-fluorouracil in saliva using surface-enhanced Raman spectroscopy” Vibrational Spectroscopy 38 79-84 2005 3 Farquharson, S., Shende, C., Inscore, F., Maksymiuk, P., Gift, A “Analysis of 5-fluorouracil in saliva using surface-enhanced Raman spectroscopy” J. Raman Spectrosc., 36, 208-212 2005 4 Inscore, F., Shende, C., Maksymiuk, P., Farquharson, S., “Ten-minute analysis of drugs and metabolites in saliva by surface-enhanced Raman spectroscopy” Proc. SPIE, 6007 2005 5 Gift, A., Shende, C., Inscore, F., Farquharson, S “Analysis of the chemotherapy drug 5-fluorouracil and its metabolites by surface- enhanced Raman spectroscopy” Proc. SPIE, 5588, 70-77 2005 6 Inscore, F., Shende, C., Maksymiuk, P., Farquharson, S. “Astronaut health monitoring” Proc. SPIE 2006

5. A2.1

Appendix 2: Career - Journal Articles, Publications, Papers, Book Chapters, Abstracts

feinscore@gmail.com

7 Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S. “Rapid analysis of drugs in saliva using a disposable lab-on-a-chip” Proc. SPIE 2006 8 Farquharson, S., Gift, A., Shende, C., Inscore, F., Ordway, B., Farquharson, C., Murren, J., “Surface-enhanced Raman spectral measurements of 5-fluorouracil in saliva” Molecules, 13, 2608-2627 2008 9 Inscore, F., Shende, C., Farquharson, S. “Astronaut health monitoring and Urine Analysis” NASA Medical tech briefs (2010) 10 Inscore, F., Shende, C., Sengupta, A., Huang, H., and Farquharson, S “Detection of Drugs of Abuse in Saliva by Surface-enhanced Raman spectroscopy” Applied Spectroscopy 65, 1004-1008 2011 11Farquharson, S, Shende, C., Sengupta, A., Huang, H., and Inscore, F “Rapid Detection and Identification of Overdose Drugs in Saliva by Surface-enhanced Raman Scattering Using Fused Gold Colloids” Pharmaceutics, 3, 425-439 2011 12Inscore, F., Shende, C., Huang, H., Sengupta, A., and Farquharson, S “Detection and identification of drugs of abuse in saliva” In: S. Farquharson, Ed., Applications of Surface-Enhanced Raman Spectroscopy (CRC Press, 2013, Chapter 9, in press) Biological Agents/Pathogens Assays in air, surfaces, water, food, biofluids 1 Farquharson, S., Gift, A., Maksymiuk, P., Inscore, F. “Rapid dipicolinic acid extraction from Bacillus spores detected by surface- enhanced Raman spectroscopy” Applied Spectroscopy., 58, 351- 354 2004 2 Inscore, F., Gift, A., Farquharson, S “Detect-to-treat: development of analysis of Bacilli spores in nasal mucus by surface-enhanced Raman spectroscopy” Proc. SPIE, 5585, 53-57 2005 3 Farquharson, S., Smith, W., Brouillette, C., Inscore, F “Detecting Bacillus spores by Raman and surface-enhanced Raman spectroscopy”, Spectroscopy., June supplement, 8-15 2005 4 Farquharson, S., Inscore, F “Detection of invisible bacilli spores on surfaces using a portable SERS-based analyzer” Internal. J. of High Speed Electronics and Systems 296-306 2007 5 S. Farquharson, C. Shende, A. Gift, and F. Inscore “Detection of Bacillus spores by surface-enhanced Raman spectroscopy” In Bioterrorism, Stephen S. Morse, Ed., ISBN 978-953-307-636-2 (2012) 6 C. Shende, A. Sengupta, H. Huang, S. Farquharson, F. Inscore “Detection of Bacillus spores within 15 minutes by SERS” Proc SPIE, 8358-15 (May 2012) 7 Atanu Sengupta, Chetan Shende, Stuart Farquharson, and Frank Inscore, “Detection of Bacillus anthracis-Sterne Spores Using Peptide Functionalized SERS-Active Substrates” Int J Spectrosc, on-line August 2012 8 Sengupta A, C Shende, H Huang, S Farquharson, F Inscore, “Rapid analysis of foodborne pathogens by SERS” Proc SPIE, 8369-19 (2012) 9 Inscore, F., Sengupta, A., Shende, C., Dana, K., Huang, H., and Farquharson, S, “SERS assay for Bacillus anthracis, Yersinia pestis, Clostridium botulinum toxin A and other bioagents” (in 2013 DARPA P2 final report) 10 S Farquharson, C Shende, W Smith, H Huang, F Inscore, A Sengupta, J Sperry, T Sickler, A Prugh, J Guicheteau; “Selective detection of 1000 B. anthracis spores within 15 minutes using a peptide functionalized SERS assay” Analyst 139 6366 (2014) Food Adulterants 1 Hermes Huang, Chetan Shende, Atanu Sengupta, Frank Inscore, Carl Brouillette, Wayne Smith and Stuart Farquharson “SERS of Melamine and Other Chemicals Using a 1550 nm (Retina-Safe) Laser” Journal of Raman Spectroscopy 43, 01-705, 2012 Microplates/DNA,RNA, Biologicals, Proteims, Drugs, Chemical agents, Explosives 1 Drapcho, D., Zlatkin, I., Inscore, F., Shende, C., Sengupta, A., Huang, H., and Farquharson, S. “High Throughput Trace Analysis Using SERS-Coated Microtiter Plates with a Raman Plate Reader” Spectroscopy (Special issue), 42-50 June 2010 Microreactors/Drug synthesis 1 Farquharson, S., Shende, C., Maksymiuk, P., Inscore, F “Real-time control of a small-scale reactor by Raman spectroscopy (a NeSSI application)” Proc. SPIE 6371 2006 Chemometrics/Fuels 1 Inscore, F., Farquharson, S., Smith, W “Determination of jet and diesel fuel properties by chemometrics using a portable Raman analyzer”, Proc. SPIE 6371 (2006) feinscore@gmail.com 6. A2.2

Publications continued

PUBLICATIONS (and Abstracts/Presentations) as Post-doc/Graduate Student 1 Inscore, F., Knottenbelt, S., Rubie, N., Joshi, H., Kirk, M., Enemark, J., “Understanding the origin of metal-sulfur vibrations in an oxo molybdenum dithiolene complex: relevance to sulfite oxidase”, Inorg. Chem., 45, 967 (2006) 2 Joshi, H.K., Cooney, J.J.A., Inscore, F.E., Gruhn, N.E., Lichtenberger, D.L., Enemark, J.H. “Investigation of Metal-Dithiolate Fold Angle Effects: Implications for Molybdenum and Tungsten Enzymes”, Proc. Nat. Acad. Sci., 100, 3719-3724, (2003) 3 Wang, X.B., Inscore, F.E., Yang, X., Cooney, J.J.A., Enemark, J.H., Wang, L.S., “Probing the electronic structure of [MoOS4]- centers using anionic photoelectron spectroscopy”, J. Am. Chem. Soc., 124, 10182, (2002) 4 Inscore, F.E., McNaughton, R., Westcott, B.L., Helton, M., Jones, R., Dhawan, I.K., Enemark, J.H., Kirk, M.L.,“Spectroscopic Evidence for a Unique Bonding Interaction in Oxo-Molybdenum Dithiolate Complexes: Implications for sigma- Electron Transfer Pathways in the Pyranopterin Dithiolene Centers of Enzymes”, Inorg. Chem. 38, 1401-1410, (1999) 5 Jones, R.M., Inscore, F.E., Hille, R., Kirk, M.L., “Freeze-quench magnetic circular dichroism spectroscopic study of the very rapid intermediate in xanthine oxidase”, Inorg. Chem., 38, 4963, (1999) 6 Malamatari, D., Hitou, P., Hatzidimitriou, A., Gourdon, A., Inscore, F., Kirk, M., Kessissoglou, D., “First example of a mixed valence MnIIIMnIIMnIII Schiff-Base polymeric complex having a trimeric repeat unit, crystal structure of [Mn3(HL)2(acetato)2(5-Cl salicylato)2]n”, Inorg. Chem., 34, 2493-2494, (1995) INVITED PAPERS (Special Dedication Editions) as Post-doc 1 Inscore, F.E., Joshi, H., McElhaney, A., Enemark, J.H., “Remote ligand substituent effects on the properties of Oxo-Mo(V) centers with a single ene-1,2-dithiolate ligand”, Inorg. Chim. Acta., 2002, 331, 246-256 (Geof Sykes) 2 Hemant K. Joshi, Frank E. Inscore, Julien T. Schirlin, Ish K. Dhawan, Michael D. Carducci, Tonja G. Bill and John H. Enemark, “Six- coordinate molybdenum nitrosyls with a single ene-1,2-dithiolate ligand”, Inorg. Chim. Acta., 2002, 337, 275-286 (Karl Wieghardt) 3 McElhaney, A.E., Inscore, F.E., Schirlin, J.T., Enemark, J.H., “Electron transfer studies of dithiolate complexes: effects of ligand variation and metal substitution”, Inorg. Chim. Acta., 2002, 341, 85-90 (Kenneth Raymond) ABSTRACTS for ACS Conferences as Graduate/Post-doc 1 John H. Enemark, Hemant K. Joshi, Frank E. Inscore, Julien T. Schirlin, Michael D. Carducci, Anne E. McElhaney, Ish K. Dhawan, Tonja G. Bill, Nadine E Gruhn, and Dennis Lichtenberger, “Dithiolate fold angle variations in trispyrazolylborate molybdenum compounds containing axial oxo or nitrosyl ligands”, ACS abstract, INOR, presented at ACS National Meeting, New Orleans, LA, May 23, 2003 2 Hemant K. Joshi, J. Jon A. Cooney, Frank E. Inscore, Nadine E. Gruhn, Dennis L. Lichtenberger, and John H. Enemark, “Metallacycle Folding in Metal-Dithiolate Compounds: Implications for Molybdenum and Tungsten Enzymes”, ACS abstract, INOR, presented at ACS National Meeting, New Orleans, LA, May 23, 2003 3 Schirilin, J.T., Inscore, F.E., Joshi, H.K., Dhawan, I.K., and Enemark, J.H.,“Molecular structure and properties of molybdenum nitrosyls with ene-1,2-dithiolate ligands” ACS abstract, INOR 505, 221 at ACS National Meeting, San Diego, CA, April 1-5, 2001. 4 Inscore, F.E., Hill, J.P., Young, C.G., Westcott, B.L., Enemark, J.H., Kirk, M.L.,“Electronic Structure Studies of Mo and W Dithiolates”, Abstracts of Papers, The American Chemical Society, V217,(pt1) pp. 473-INOR, presented at the National ACS Meeting- Anaheim CA, 3/21-3/25 1999 5 Inscore, F.E., Dhawan, I.K., Westcott, B.L. Enemark, J.H. Kirk, M.L.,“Electronic Structure Studies of Monooxo-Mo(V) Dithiolene Complexes: Insight into the Electron Transfer Pathway in DMSO Reductase”, Abstracts American Chemical Society, v.213 pp. 481b- INOR, National ACS, San Francisco, CA, 4/13-4/17 1997 ABSTRACTS for Gordon Conferences as Graduate/Post-doc 1 Joshi, H.K., Inscore, F.E., Schirilin, J.T., Gruhn, N.E., and Enemark, J.H.,“Fold Angle Variation Effects on Electronic Properties of Oxo- Mo, Oxo-W and Nitrosyl-Mo Ene-1,2-Dithiolate Systems: A Possible Regulatory Role within the Pyranopterin Mo and W Enzymes”, presented at the Gordon Conference, Ventura, CA, 2002 2 Inscore, F., Hill, J., Young, C., Enemark, J., Kirk, M.,“Electronic Structure Contributions to Reduction Potentials and Oxygen Atom Transfer Reactivity in Oxo-Mo and Oxo-W Dithiolates: Insight into Tungsten and Molybdenum Pyranopterin Enzymatic Reactivity Differences”, Gordon Conference, Ventura, CA, 2001 3 Inscore, F.E., Jones, R., Helton, M.E., Basu, P., Enemark, J.H., Kirk, M.L.,“Electronic Structure Contributions to Reactivity in Pterin- Containing Molybdenum Enzymes”, presented at the Metals in Biology Gordon Conference, Oxnard, CA, January 1998 feinscore@gmail.com 7. A2.3

Publications continued

SPIE: The International society for optical Engineering

2012 (Philadelphia, PA) 1. Huang H, C Shende, A Sengupta, F Inscore, S Farquharson, “Evaluation of SERS substrates for chemical agent detection” 2. C. Shende, A. Sengupta, H. Huang, S. Farquharson, F. Inscore “Detection of Bacillus spores within 15 minutes by SERS” 3. Sengupta A, C Shende, H Huang, S Farquharson, F Inscore, “Rapid analysis of food-borne pathogens by SERS” 2007 (Orlando, Fla) 1. Inscore, F., Farquharson, S., “Water security: continuous monitoring of water distribution systems for chemical agents by SERS” Optics and Photonics in Global Homeland Security III 2006 (Boston, MA) 1. Inscore, F., Farquharson, S., “A SERS-based analyzer for point and continuous water monitoring of chemical agents and their hydrolysis products” 2. Inscore, F., Shende, C., Maksymiuk, P., Farquharson, S., “Rapid analysis of drugs in saliva using a disposable lab-on-a-chip” 3. Inscore, F., Shende, C., Farquharson, S., “Trace detection of bacilli spores on surfaces using a portable SERS-based analyzer” 4. Inscore, F., Shende, C., Maksymiuk, P., Farquharson, S., “Astronaut health monitoring” 5. Farquharson, S., Shende, C., Maksymiuk, P., Inscore, F., “Real-time control of a small-scale reactor by Raman spectroscopy (a NeSSI application)” 6. Inscore, F., Farquharson, S., Smith, W. “Determination of jet and diesel fuel properties by chemometrics using a portable Raman analyzer” 2005 (Boston, MA) 1. Inscore, F., Farquharson, S., “Detecting hydrolysis products of chemical agents in water by surface-enhanced Raman spectroscopy” 2. Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S., “Ten-minute analysis of drugs and metabolites in saliva by surface-enhanced Raman spectroscopy” 2004 (Philadelphia, Pa) 1. Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S., “Characterization of chemical warfare agent hydrolysis products by surface- enhanced Raman spectroscopy” 2. Inscore, F., Shende, C., Gift, A., Maksymiuk, P., Farquharson, S., “Analysis of Bacilli spores in nasal swabs by surface-enhanced Raman spectroscopy” 3. Shende, C., Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S., “Analysis of pesticides on or in fruit by surface-enhanced Raman spectroscopy” 4. Gift, A., Shende, C., Inscore, F., Farquharson, S., “Analysis of the chemotherapy drug 5-fluorouracil and its metabolites by surface- enhanced Raman spectroscopy” 2003 (Providence, RI) 1. Shende, C., Gift, A., Inscore, F., Maksymiuk, P., Farquharson, S., “Inspection of pesticide residues on food by surface-enhanced Raman spectroscopy” 2. Gift, A., Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S., “Five minute analysis of chemotherapy drugs and metabolites in saliva: Evaluating Dosage” 3. Farquharson, S., Gift, A., Maksymiuk, P., Inscore, F., Smith, W., Morrisey, K., Christesen, S., “Chemical agent detection by surface- enhanced Raman spectroscopy” 4. Farquharson, S., Gift, A., Maksymiuk, P., Inscore, F., Smith, W., “pH dependence of methyl phosphonic acid, dipicolinic acid, and cyanide by surface-enhanced Raman spectroscopy” PITTCON: The Pittsburg Conference March 2013 Philadelphia, PA 1. Frank Inscore, Chetan Shende, A. Sengupta, H. Huang, K. Dana, S. Farquharson, “B. anthracis spores, Y. pestis bacteria and C. botulinum A toxin detection by field usable SERS assay” 2. Atanu Sengupta, Chetan Shende, Hermes C Huang, Stuart Farquharson, Frank E Inscore, “Detection of Pathogens in Food Using a SERS-Based Assay” 3. Hermes Huang, Stuart Farquharson, Chetan Shende, Atanu Sengupta, Frank E Inscore, “Evaluation of SERS Substrates and Enhancement Factors Used to Characterize Them” 2012 Orlando, Fla 1. F Inscore, C Shende, A Sengupta, S Farquharson “Detection of Single-Digit Bacillus Anthracis Spores in 15 Minutes by SERS” 2. F Inscore, C Shende, A Sengupta, S Farquharson “Rapid Detection and Identification of Overdose Drugs in Saliva by SERS” 3. H. Huang, C. Shende, A. Sengupta, F. Inscore, S Farquharson “Raman Spectroscopy Using Retina-Safe (1550 nm) Laser Excitation” 4. F. Inscore, C. Shende, A. Sengupta, S Farquharson “Rapid Analysis of Food-borne Pathogens” 2011 Atlanta, GA 1. S. Farquharson, F. Inscore “Field Analysis by SERS: Drugs, Explosives, Poisons and More”

Appendix 3: Professional Conferences and Presentations with RTA

feinscore@gmail.com 8. A3.1

2010 Orlando, Fla 1. Frank Inscore, Chetan Shende, Atanu Sengupta and Stuart Farquharson “Rapid Analysis of Drugs, Biomarkers and Pathogens in Urine, Saliva and Nasal Mucus Using a Disposable Lab-on-a-chip” 2. RTA staff, “Accurate Identification of Powders: the Power of x-axis Stability” 3. RTA staff, “A Portable Fuel Analyzer” 4. RTA staff, “Biodiesel Reactors: Raw Material Analysis, Process Control, and Product Quality” 5. F. Inscore, C. Shende, A. Sengupta, “Rapid Analysis of Drugs in Saliva using a Disposable Lab-on-a-Chip” 6. F. Inscore, C. Shende, “Water Security: Detecting Chemical Agents by SERS” 7. RTA staff, “Highly Sensitive SER-active Sol-gel Substrates” 2009 1. Frank Inscore, Chetan Shende, Atanu Sengupta, S Farquharson “Securing Our Nations Water Supplies: Detecting Poisons by SERS” 2. C Shende, Frank Inscore, S Farquharson, J Stuart “Detection of Part-per-billion Chlorpyrifos-Methyl in Orange Juice” 2008 (New Orleans, La) 1. Frank Inscore, C Shende, A. Sengupta, S Farquharson “Water Security: Detecting Chemical Agent Hydrolysis Products by SERS” 2007 1. Frank Inscore, C Shende, S Farquharson “Monitoring Astronaut Health: Detection of Preventative Drugs in Urine by a SERS-Chip” 2006 (Orlando, Fl) 1. Inscore, F., Farquharson, S., “Detecting hydrolysis products of chemical agents in water by surface-enhanced Raman spectroscopy” 2. Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S., “Five-minute analysis of drugs and metabolites in saliva by SERS” 2004 (Chicago, Il) 1. Farquharson, S., Maksymiuk, P., Gift, A., Inscore, F., “Chemical and biological agent detection by surface-enhanced Raman spectroscopy” (Oral, F.I.). 2. Shende, C., Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S., “Inspection of pesticide residues on food by surface-enhanced Raman spectroscopy” 3. Shende, C., Gift, A., Inscore, F., Maksymiuk, P., Smith, W., Farquharson, S., “Reproducible SERS: chemical agent detection”. 4. Farquharson, S., Griegly, L., Maksymiuk, P., Gift, A., Inscore, F., Shende, C., Smith, W., Sperry, J.Fenerty, G., “Detection of anthrax on a mail sorting system using Raman (and surface-enhanced Raman spectroscopy)” 5. Inscore, F., Shende, C., Gift, A., Maksymiuk, P., Farquharson, M., Farquharson, S., “Rapid chemical separation with simultaneous surface-enhanced Raman spectral analysis” 6. Gift, A., Shende, C., Inscore, F., Farquharson, S., “Speeding drugs through clinical trials, Five-minute analysis of drugs and metabolites in saliva” FACSS: The Federation of Analytical Chemistry and Spectroscopy Studies 2004 (Portland, OR) 1. Inscore, F., Gift, A., Farquharson, S., “Rapid detection of chemical agent hydrolysis products by surface-enhance Raman spectroscopy” 2. Inscore, F., Shende, C., Gift, A., Farquharson, S., “Monitoring astronaut health: urinalysis by surface-enhanced Raman spectroscopy” 3. Farquharson, S., Gift, A., Inscore, F., “Analysis of Bacilli spores in nasal swabs by surface-enhanced Raman spectroscopy” (Poster). 4. Gift, A., Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S., “Chemotherapy drug analyzer: fast chemical analysis by surface- enhanced Raman spectroscopy” (Poster). 5. Shende, C., Gift, A., Inscore, F., Farquharson, S., “Rapid saliva analysis for clinical trials” (Poster). EAS: Eastern Analytical Symposium 2011 NJ 1. F. Inscore, C. Shende “Drugs in Saliva” 2. RTA Staff, plus Montgomery Shaw, Chitra Subramanian, University of Connecticut “Monitoring Cure of Compression Molded Composites using Raman Spectroscopy” 3. RTA Staff plus Montgomery Shaw, Chitra Subramanian, University of Connecticut “Cure of Compression Molded Composites using Raman Spectroscopy” 4. RTAStaff plus James Stuart, Richard Parnas, University of Connecticut “Calibrating Batch Process Biodiesel Production with Raman Spectroscopy” 5. RTA Staff, “Pharmaceutical Drug Synthesis and Process Monitoring by Raman Spectroscopy” 6. RTA Staff, “A Raman-Based Portable Fuel Analyzer” 7. RTAStaff, “Eye Safe Raman at 1550 nm” 2010 NJ 1. RTA Staff, “Pharmaceutical Analysis from Start to Finish by Raman Spectroscopy” 2. RTA Staff, “High-Throughput Trace Analysis using SERS-Active Microplates” 3. RTA Staff, “Forensic Analysis by SERS: Drugs, Poisons, Explosives, and More” 4. RTA Staff, “Fuel Analysis: 18 Chemical and Physical Properties in less than 5 Minutes, Anywhere” feinscore@gmail.com 9. A3.2

Presentations continued

2008 (Somerset, NJ) 1. F Inscore, Chetan Shende, A Sengupta, S Farquharson “Water Security: Detecting Chemical Agent Hydrolysis Products by SERS” 2. C Shende, F Inscore, A Sengupta S Farquharson “LIFE ON MARS: amino acid detection using SERS-ACTIVE LAB-ON-CHIP” 2006 (Somerset, NJ) 1. Inscore, F., Farquharson, S., “A SERS-based analyzer for point and continuous water monitoring of chemical agents and their hydrolysis products” 2. Shende, C., Inscore, F., Maksymiuk, P., Farquharson, S., “Rapid analysis of drugs in saliva using a disposable lab-on-a-chip” 3. Inscore, F., Farquharson, S., “Trace detection of bacilli spores on surfaces using a portable SERS-based analyzer” (Oral, F.I.). 4. Inscore, F., Shende, C., Maksymiuk, P., Farquharson, S., “Astronaut health monitoring”. 5. Farquharson, S., Shende, C., Maksymiuk, P., Inscore, F., “Real-time control of a small-scale reactor by Raman spectroscopy (a NeSSI application)” 6. Shende, C., Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S., “Analysis of pesticides on or in fruit by surface-enhanced Raman spectroscopy” 2005 (Somerset, NJ) 1. Inscore, F., Farquharson, S., “Detection of chemical agents and their hydrolysis products in water by SERS 2. Inscore, F., Chende, C., Maksymiuk, P., Farquharson, S., “Five-minute analysis of drugs and metabolites in saliva; speeding drugs through clinical trials” 2004 (Somerset, NJ) 1. Inscore, F., Gift, A., Maksymiuk, P., Farquharson, S. “Rapid analysis of chemical agent hydrolysis products by surface-enhance Raman spectroscopy” 2. Inscore, F., Chende, C., Maksymiuk, P., Gift, A., Farquharson, S., “Rapid analysis of urine by surface-enhanced Raman spectroscopy: Monitoring astronaut health” IFPAC: PAT 2013 (Process Analytical Technology) 1. Chetan Shende, Hermes, Huang, Stuart Farquharson, and Frank Inscore, “Rapid Identification of Overdose Drugs in Saliva by Surface-enhanced Raman Spectroscopy” 2. Atanu Sengupta, Chetan Shende, Stuart Farquharson and Frank Inscore, “Detection of Pathogens in Food using SERS-based Assay” 2012 (Process Analytical Technology) 1. RTA staff, “Retina Safe (1550 nm) Portable Raman Spectroscopy” 2. C Bruollette, Frank Inscore, M Donahue, S Farquharson, W Smith “18 Fuel Properties Determined in 3 Minutes by Raman Spectra” 3. A Sengupta, C Shende, H Huang, S Farquharson Frank Inscore “Foodborne Pathogen Analysis by SERS” 4. Chetan Shende, Frank Inscore, Atanu Sengupta, Hermes Huang and Stuart Farquharson “Detection of DEHP, Melamine, and Pesticides in Liquid and Solid Food Samples by SERS” 5. RTA staff, “Monitoring Biodiesel Production: From Lab to Plant” 6. F Inscore, C. Shende, A Sengupta, H Huang S Farquharson “Detection of Illicit Drugs in Impaired Driver Saliva by Handheld SERS” 2011 (Process Analytical Technology) 1. RTA Staff, “Biodiesel Reactors: Raw Material Analysis, Process Control, and Product Quality” 2. RTA Staff “Pharmaceutical Analysis from Start to Finish by Raman Spectroscopy” 3. RTA Staff, “Forensic Analysis by SERS: Drugs, Poisons, Explosives, and More” 4. RTA Staff, “Eye Safe Raman at 1550 nm” 5. RTA Staff, “High Throughput Trace Analysis using SERS-Active Microplates” 6. RTAStaff, “Rapid Analysis of Food Contamination by SERS: Melamine & Pesticides” 2010 (Process Analytical Technology) 1. RTA Staff, “Biodiesel Reactors: Raw Material Analysis, Process Control, and Product Quality” 2. RTA Staff, “Accurate Identification of Powders: The Power of X-Axis Stability” 3. RTA Staff, “A Portable Fuel Analyzer” 4. Inscore, Shende, Farquharson, “Water Security: Detecting Chemical Agents by SERS using NeSSI” OTHER PRESENTATIONS, CONFERENCES and PROFESSIONAL MEETINGS for RTA NSF DMII Grantees Conference: 2005 (Tempe, AZ) 1. F. Inscore (CI) and S. Farquharson (PM), NSF Phase II SBIR presentation of results and commercialization plans for “Anthrax detector for mail sorting systems” 2. F. Inscore (CI) and S. Farquharson (PM), NSF Phase II SBIR presentation to potential investment partners for “Anthrax detector for mail sorting systems” Yale University (Dept. of Physics, Prof. Richard Chang): 2005 (New Haven, CT) 3. S. Farquharson, W. Smith, and F. Inscore; spore detection and SERS presentation (Collaboration Meeting). NASA Glenn Research Space Center: 2004 (Cleveland, OH) 4. F. Inscore (PI) and S. Farquharson (PM), NASA Phase I SBIR results "On-demand urine analyzer"

Presentations continued

feinscore@gmail.com 10. A3.3

Patents - hold 4 patents at RTA: “Method & apparatus for 2 step SERS Assays” #20130157254, June 2013 “Method effecting rapid release of signature chemical from bacterial endospores, and Detection thereof” #7713914, May 2010 “SERS method and apparatus for rapid extraction and analysis of drugs in saliva” U.S. Patent Number 7393691, July 2008 “SERS method rapid pharmacokinetic analysis drugs in saliva” U.S. Patent Number 7393692, July 2008 +1 in progress / +assisted in additional 5 awarded to RTA 2003 – 2015 Title Ph.D. Dissertation Published: “Electronic Structure Studies of Mo- and W-dithiolate Complexes: Electronic Structure Contributions to Reactivity in Pyranopterin Molybdenum and Tungsten Enzymes” PhD / Doctor of Philosophy / Chemistry, conferred on May 5, 2000 Doctorate Defense Committee: Prof. M. Kirk, (Mentor/Advisor) Dept. Chem.; Bio-Inorganic Chemistry UNM Prof. M. Ondrias, (Bell Lab Fellow) Dept. Chem.; Physical Chemistry UNM & Sandia National Laboratories (SNL) NM Prof. E. Walters, (Regent Chair) Dept. Chem.; Physical Chemistry UNM Prof. G. Kynner, (Emeritus, Brown Chair) Dept. Math & Computer Programming for Engineers; Applied Mathematics UNM Prof. M. Hampden-Smith, Dept. Chem.; Inorganic Chemistry UNM & National Institute of Materials and Surfaces NM Prof. F. Allen, (Dept Chair), Dept. Chem.; Analytical and Physical Chemistry UNM, founder of Chromex (bought out by Kaiser) Dr. P.J. Hay, Theoretical Division; Computational Chemistry Group, Los Alamos National Laboratory (LANL) NM

Appendix 4: Patents Awarded with RTA

feinscore@gmail.com 11. A4.1 A5.1

Appendix 5: PhD Dissertation & Defense

APPENDIX 6 Contact References for FEI (2015) Mr. Craig Silverman Analytical Instrumentation Sales csilv@aol.com 860-985-0782 Craig was previously the senior sales and marketing director at rta, I worked with him closely. He has worked for a number of large corporations (Jasco, Thermo, etc.), and is well known in the Raman and the GC/MS/HPLC industry for chemistry applications. I have known Craig for over 5yrs Mr. Chetan Shende Senior research chemist (MS) at RTA, Middletown CT 860-202-0321 I have supervised and been intimately associated with Chetan over 10 years. Dr. Hemant K. Joshi (colleague and collaborator) hkjoshi@gmail.com 913-952-6291 Scientist, Entrepreneur, Author 7908 W 142 St. Overland Park, KS 66223 520-903-1705 and Principle Investigator, Nika Biochemicals Inc., Olathe, Kansas I have known Hemant for 14 years since my Post-Doc at UAZ, where he was a top honors graduate student I had the privilege to mentor and coach in the Enemark Group. He has worked at a number of large biotech companies, started his own business (using SBIR funding and private investments), and now seeking a tenure track faculty position in theoretical / computational chemistry. . Mr. Wayne Smith VP at RTA (product development, soft-ware development) Wayne Smith <wwsmith2@gmail.com> 860-918-0441 I have worked with Wayne over last 10 yrs, reported to / and collaborated with him Regents Prof. John Enemark for 3 years (2000-2003) postdoc at the University of Arizona, Dept of Chem. Tucson AZ. Prof Enemark is now Emeritus, but can be reached by: Cell 520-425-7210 Lab 520-621-2245 Email jenemark@email.arizona.edu

APPENDIX 7 RECOMMENDATIONS in my LinkedIn Profile www.linkedin.com/in/finscore2014

LinkedIn 50 Skills (with 450 peer endorsements of): spectroscopy, chemistry, analytical chemistry, nanotechnology, organic chemistry, characterization, nanomaterials, Raman, chromatography, biochemistry, UV/Vis, materials science, nanoparticles, NMR, inorganic chemistry, laboratory, science, scientific writing, physical chemistry, electrochemistry, assay development, R&D, research, data analysis, biotechnology, molecular biology, SERS, catalysis, HPLC, sensors, powder X-ray diffraction, purification, IR, surface chemistry, gas chromatography, FTIR, mass spectrometry, biosensors, sol-gel, lifesciences, optics, physics, formulation, design of experiments, experimentation, organic synthesis, fluorescence, chemometrics, computational chemistry, organometallic chemistry

LinkedIn 9 Recommendations:

- Dr. Frank Inscore, Director R&D, Raman and SERS Applications, Real-Time Analyzers, Inc.

Wayne Smith, Raman Spectroscopy Specialist & Certified LabVIEW Programmer “ Frank is a detail-oriented manager and researcher. His knowledge of chemistry and mathematics, and his research experience enabled him to successfully lead the SERS group. With his leadership, the group developed some of the best commercially available SERS substrates on the market. October 7, 2013, Wayne worked with Frank at Real-Time Analyzers, Inc.

Carl Brouillette, Product Development Engineer at Real-Time Analyzers “Frank is a very enthusiatic and energetic researcher. His work always fascinates me! December 1, 2012, Carl worked with Frank at Real-Time Analyzers, Inc.

Paul Maksymiuk, Chemistry Tutor at Central New Mexico Community College “ While working at Real-Time Analyzers, Dr. Frank Inscore served as my supervisor as well as mentor. Dr. Inscore is an excellent leader who knows how to lead numerous research projects and delegate tasks so that high quality research is always being completed. His hard work and dedication are a pronounced driving force that has propelled research resulting in the completion of numerous patents and publications. Under his direction I was constantly pushed to be a more objective, analytical, and an overall better scientist. Furthermore, Dr. Inscore has clear communication with his team and always knows and recognizes where everyone’s contribution fits. Dr. Inscore is an superior team leader and is definitely a valued asset to the Real-Time Analyzers Company. March 12, 2012, Paul reported to Frank at Real-Time Analyzers, Inc.

Craig Silverman, Analytical Instrumentation Sales “ Frank is an outstanding scientist and colleague who was a pleasure to work with during my time at RTA. He consistently demonstrated strong technical and interpersonal skills and was a vital member of RTA's R & D department. He brings a strong work ethic and a high level of enthusiasm to every project he is involved with.

I highly recommend Frank as a colleague and would be happy to work with him again. January 16, 2012, Craig worked with Frank at Real-Time Analyzers, Inc.

Michael Patient, Software Engineer at Space Exploration Technologies “ Frank works tirelessly and plays a crutial role in the development of our company. December 22, 2011, Michael worked with Frank at Real-Time Analyzers, Inc.

Dr. Atanu Sengupta, Senior Scientist, Group Lead, Spectroscopy, Dr. Reddy's Laboratories “ I know Frank for the last five years and it has been a great time working with him. Frank's contribution to the development of SERS at RTA is huge... and his approach to critical problems is also worth praising.... He has this unique ability to disect a problem and come up with a solution... As a team leader he has been very successful in motivating his fellow team members. In a nutshell, I can describe Frank as an extremely talented researcher, a great team leader and a fun person to work with. December 13, 2011, Atanu reported to Frank at Real-Time Analyzers, Inc.

Dr. Mike Donahue, Applied spectroscopist “ Frank Inscore has played a vital role in Real-Time Analyzer's development of Surface Enhanced Raman Spectroscopy (SERS) substrates and applications. Having been a program manager on several successful products utilizing SERS, he has brought practical commercial potential to the forefront of this emerging technology. Further, having been involved with chemometric software development for Real-Time Analyzer's Portable Fuel Analyzer, Frank has demonstrated adept skill for modeling of spectral data toward property prediction and screening for trace contamination. Frank is now bridging these data processing capabilities to enable modeling and library analytics to the SERS Analyzer. Frank's ability to assist both the core science and practical commercial aspects has helped to make Real-Time Analyzer's a world leader in SERS applications. December 12, 2011, Mike reported to Frank at Real-Time Analyzers, Inc.

Chetan Shende, Research Chemist at Real-Time Analyzers, Inc. “ Dr. Frank Inscore is an extremely well qualified, talented, intelligent and hard working person. His detail oriented and forward thinking approach ensures successful and timely completion of projects. Dr. Inscore's problem solving and leadership abilities are a big asset to our research group. To sum it up Dr Inscore is a compassionate and smart person who is a pleasure to work with. December 6, 2011, Chetan reported to Frank at Real-Time Analyzers, Inc.

- Dr. Frank Inscore, Post-doctoral Research Fellow (Senior Scientist/Chemist) University of Arizona

Dr. Hemant Joshi, CEO/ Co-Founder/ Nika Biochemicals “ Dr. Inscore is very proactive ensuring that he is abreast with the latest in his field of application. He is one of the top scientists in the country in Raman spectroscopy and its applications. He was an excellent coach/mentor providing cutting edge training, guidance and timely advice during our two years of professional interaction. He takes full ownership of task at hand, communicates in very timely manner and is a very reliable resource. He is pleasant to work with and his willingness to help is remarkable. November 26, 2011, Hemant worked directly with Frank at University of Arizona

APPENDIX 8

Leadership Statement

“My philosophy of leadership is one that exemplifies an effective leader as a strong role-model for subordinates & employees across the organization, who demonstrates vision, courage, confidence and

commitment while connecting with and inspiring others to achieve extraordinary results through teamwork”

Leadership is about influencing people. The effective leader must have the ability to inspire a shared vision of purpose (provide team with clear vision and strategic direction), provide an environment which enables others to act effectively, demonstrate to others that their commitment can and does make a difference, and provide the self-satisfaction rewards that promote commitment and dedication.

I am recognized as a tireless take-charge individual lifelong learner, who shares experiences with newest to most senior staff to make all part of total operational goal of department, recognizes own limitations and prepared to be a skilled follower. NOTE possess specific but essential skills and comprehensive knowledge of the field; first rate communicator and listener, self promotes core values accountability discipline ethical behavior and employee empowerment – thus ensures credibility and relationships maintained with workers; realize that effective leaders learn from those around them, and adapt their leadership style (e.g. modify lead by example/participatory model) as necessary to deal with diverse personalities and evolving situations, and must be sensitive of actions and their impact on followers. RESULT can identify and leverage strengths of individuals to improve team performance / spirit, producing future leaders

In summary; leadership is the accomplishment of goals (vision) through others. It is the ability to help individuals (or organizations) surpass themselves and their abilities, and to motivate them to exceed and succeed in their personal as well as the organizations goals. I believe a leader develops visions for the organization and a roadmap on how to get there. A leader views roadblocks only as hurdles to overcome, and continues on and remains on course. A leader must get commitment on the visions with others in the organization so all are in line “following the same path”