Histopathology Results of biodistribution of SPIO in

Apo E Mouse

After 24 hours

Aortic Root (a and B) Demonstrating 8 Labeled Macrophages Adhering Circumferentially to Atherosclerotic Plaque. One Macrophage Containing 10 Fluorescent microspheres (C and D) Is Seen Adhering to a Fibrous Plaque(with hematoxylin with the use of a xylene-free method)

One macrophage containing 10 fluorescent microspheres (c and d) is seen adhering to a fibrous plaque. One aggregation of 4 labeled macrophages (e and f) is seen attached to an advanced plaque. Numerous macrophages (g and h) are seen adhering to this isolated fatty streak, which is mostly comprised of foam cells. A collection of foam cells overlying a fibrous plaque attracts labeled macrophages (i and j). A few microspheres are seen within the area of foam cells, suggesting transmigration of the macrophage across the endothelial cell layer.

Transvers section Descending Aorta

Movat’s stainingPearl’s

Transvers section Descending Aorta(diaphragm level)

H&EPearl’s

Transvers section Descending Aorta (diaphragm level)

CD60Pearl’s

Transvers section Descending Aorta (diaphragm level)

CD6o H&EPearl’s

Transverse Section of Aorta Valve Region

Movat’sPearl’s

Transverse Section of Aorta Valve Region

H&EPearl’s

Transverse Section of Aorta Valve Region

CD60Pearl’s

Transverse Section of Aorta Valve Region

CD60 H&EPearl’s

Liver