100515 presentation seed support methods

Priming Technologies Introduction

Dormancy Alleviation Vigor Improvement Stress Resistance Uniformity and Quality of

Seedlings and Plants Potentially higher Yield

(sugarbeet – sugar yield)

By:

Embryonic Priming Non-Embryonic Priming Additives used in Priming

Protocols

Wide range of protocols have been developed: Vegetable seeds: lettuce, tomato, cucurbits, carrot,

fennel, brassica, spinach Agricultural seeds: grass seeds, sugarbeets, rape Flower seeds: begonia, pansy, sunflower Industrial crop seeds: tobacco

for priming, separation, disinfection and vernalizationIncluding:• Quality Testing methods• Production control methods

Priming Protocols

Flower seeds: begonia, pansy, sunflower Begonia priming: control – priming 1, 2 and 3

Priming Protocols

Sunflower seed

Priming Technologies

Priming of vegetable seeds: examples

Pepper


Priming of vegetable seeds: examples eggplant

Eggplant


Priming of vegetable seeds: examples rootstock seed (Lagenaria sp.)

Rootstock seed (Lagenaria sp.)


Priming of vegetable seeds: examples

Melon

Priming Protocols

Grass seed

Sanitation Technologies

Our aim is to sanitize the seed while at the same time to improve the germination of the seedlot :

Wet Sanitation (wet film, vapor) Priming Sanitation (in priming type processes)

Short duration Longer duration

Large scale sanitation (for larger volumes – new project) Min. capacity of large scale sanitation is 500 kg/1000 lbs per hour.

Electron beam seems too expensive

Level of sanitation (depending on the problem):

Surface sanitation Deep sanitation (incl. embryo)


Canola Sanitation for Xanthomas c.c. and other pathogens (in general a better germination possible)


Fusarium infected tomato related species:

PR = control (primed) S1 – S4 are 4 different

sanitation treatments Later additional treatments

were applied. Further potential candidates

available.


Fusarium infected tomato related species:

PR = control (primed); Left S5 is new sanitation treatment

Germination speed and Good Plants:

Object 1 d 2 d 3 d 4 d 5 d 6 d

primed 20 48 70 72 78 80

primed + San5 15 50 90 98 100 100

Object Usable Pl. 1st Qlt 2nd Qlt AbnNo Germ

primed 80 60 20 0 20

primed + San5 98 80 18 2 0

Sanitation Technologies Spinach – Verticillium control: Seed Support I and II

Sanitation Technologies Spinach – Verticillium control: Seed Support II


Germination (high)


Corn Sanitation for Fusarium and other pathogens

Biological Technologies

Aim to control some pathogens and saphrophytes during the treatment, possibly reducing mycotoxins at harvest.

And especially to reduce the chance for re-infection after germination

Endophytes to fill intracellular spaces (e.g. Bacilllus spp to control re-infection with Fusarium spp)

Trichoderma spp to cover the root system externally. Mycorrhizeae

By:

Priming-based inoculation Coating based inoculation Additives Controlled drying processes

Technologies for Parentlines(Seed Production)

Aim is to clean the seed as much as possible, even if the result is some drop in germination.

Vernalization of parentline seed in cold dependent species for improvement and synchronization of flowering

Sanitation of parentline seed for clean start of seed production

By: Priming type processes

Vernalization Protocols

Vernalization of spinach parentlines from front till back: control – stronger vernalization

Fine-tuning of seed technologies

Seed physiology is variable and effect of priming and other seed technologies may depend on varietal differences, seed production areas etc…

When priming recipe available: fine-tuning of recipe for specific genetics and green seed,

dormancy, uniformity, shelf-life, germination in stress Combinations with new technologies, e.g. upgrading,

sanitation-disinfection etc… Combinations with new coatings

Project ConsultancyEquipment, Facilities and Personnel

Facility Design Long term experience in operations allows Seed Support to offer

practical advice. For the best priming and other seed technology conditions. For optimal working conditions

Equipment Advice on selection of equipment for priming and other seed

technologies Monitoring techniques, process control techniques

Personnel Advice on suitable personnel for execution of seed technologies Advice on particular personnel if required.

100515 presentation seed support methods

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