The Impact of Platinum Production from UG2 Ore on Ferrochrome
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Transcript of 100428 ug2
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2:18
What’s DEP?
• Electrophoresis– Charged particles moving in uniform field
D ie lec tric pa rtic le
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3:18
What’s DEP?
• Dielectrophoresis– Dielectric particles moving in non-uniform field
Dielectric particle
Die lectric particle
Positive DEP Negative DEP
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4:18
What’s DEP?
• Theory bit– (the only time an equation appears in this
presentation… promise!)
232F E)K(Rer m
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**
2)(
mp
mpK
j
*
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5:18
What’s DEP used for?
• Cell movement can be manipulated by frequency changes, so DEP is good for cell collection and sorting
Morgan et al. Biophysical Journal 77 (1999) 516-525
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6:18
What’s DEP used for?
• Compare cell viability
G.H. Markx, C.L. Davey / Enzyme and Microbial Technology 25 (1999) 161–171
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7:18
What’s DEP used for?
• Compare effects of chemicals on cells
F.H. Labeed et al. / Biochimica et Biophysica Acta 1760 (2006) 922–929
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8:18
What’s DEP used for?
• Predict cell properties
232F E)K(Rer m
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9:18
What’s DEP used for?
• Everything’s fine and dandy BUT DEP is not available commercially, nor used widely– Despite being described back in 1950s
• …but why?– Small assays only
• Restricted to research use
– Time consuming• One spectrum takes 40-60 minutes
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10:18
What’s DEP used for?
• The answer(?): Multiple experiments at the same time!– Different
frequencies, or– Same frequencies
10kHz 2MHz
300um
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11:18
http://www.youtube.com/watch?v=lLZbr3vP-Zs
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12:18
DEP: The setup
• What’s needed:
Imaging/recording device
Microscope
Electrode
Cells
Signal generator
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13:18
DEP: The (new) setup
• What’s (really) needed:
Imaging/recording device
Microscope
Electrode
Cells
Multiple output signal generator
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14:18
Real-time DEP
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15:18
Real-time DEP
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-1
-0.8
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-0.4
-0.2
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Frequency (Hz)
Nor
mal
ised
ligh
t in
tens
ity
DEP plots for Jurkat cells treated with valinomycin 100402 (10 mS/m)
Control
Valinomycin
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16:18
Real-time DEP
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25-1
0
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Frequency (Hz)
DEP spectrum for Jurkat cells treated with valinomycin 091216
Time (min)
Mod
al s
hift
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17:18
Real-time DEP
• That’s it! (for now, hopefully)
• Any questions: – [email protected]– facebook.com/nahrizuladib