1 Varian 920-LC Integrated Analytical HPLC. 2 Varian 920-LC What Is It? Compact, stylish automated...
-
Upload
peregrine-davidson -
Category
Documents
-
view
229 -
download
3
Transcript of 1 Varian 920-LC Integrated Analytical HPLC. 2 Varian 920-LC What Is It? Compact, stylish automated...
1
Varian 920-LC Integrated Analytical HPLC
2
Varian 920-LC What Is It?
• Compact, stylish automated analytical HPLC
– Pre-plumbed and factory tested for the highest confidence
– Single cable communications
– Each system comes with solvent delivery system, autosampler and either UV Vis or PDA detector
– Options of a second Fl or RI detector and column heater
• Virtually pulse-free dual piston pump
– Does not require a pulse damper
• State-of-the-art low pressure quaternary gradient module with built-in degasser
3
Varian 920-LC What Is It?
• Autosampler with cooling option
– Synchronised injection for outstanding retention time reproducibility
– Compatible with 200 x 2 mL HPLC vials or 3 microplates
• Either sensitive dual UV-Vis or PDA detector
– Optional second detector - fluorescence or RI
• High precision (<0.15 °C) dual column heater available
– Low delay solvent pre-heater for improved heat transfer
– Optional column switching and electric injection valve
• NEW improved instrument interface on GalaxieTM software
4
Varian 920-LC Silver Bullets
• Fast LC with superb performance optimised for sub 3 m columns
– Improved throughput without compromising robustness
• Pulse-free solvent delivery– No need for a pulse damper
• High sensitivity detection• Full system level computer controlled AIQ (validation)
5
0.1 0.2 0.3 0.4 0.60.5 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8
mAU
RT [min]
100
90
80
70
60
110
40
30
50
20
0
10
Conditions
Mobile Phase: Water Acetonitrile GradientDetection: UV DetectionTemperature: 60°CFlow-Rate: 1,6mL/minColumn: Varian Ultra 3um 50x2,0mm
acet
ophe
none
Pro
piop
heno
ne
vale
roph
enon
e
benz
ophe
none
hexa
noph
enon
e
hept
ophe
none
octa
noph
enon
e
Varian 920-LC Fast LC Phenones
6
Varian 920-LC Fast LC PAHs
7
Varian 920-LC Fast LC Performance
• Dual piston quaternary pump produces fast gradients – even at 200 L/min
• Virtually pulse-free pumping without pulse damper
• Outstanding gradient compositional accuracy give retention time precision at the <1% level
• Built-in vacuum degas maintains pump prime and enhanced injection reliability
• Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns
• Overlay of 10 chromatograms
• 10% steps at 1 mL/min
8
Varian 920-LC Autosampler
• Needle-in-flow path geometry for low carry-over (<0.01%)
• Typical inj precision (< 0.3% at 10 L)
• Optimized flow paths for fast injection turn-around
• Synchronised injection for best RT reproducibility
• Programmable wash setting for low carry-over
• Injection volumes from 1 uL to 50 L
• Cooled option available
9
Varian 920-LC Superb Washout
Blank injection <0.01% carryover
125 µg/mL Caffeine
10
920-LC Autosampler How Does it Work?
11
Varian 920-LC Injection Synchronization
-20%
0%
20%
40%
60%
80%
100%
120%
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2
12
Sensitive UV-Vis Detectors
Typical Agilent VWD S/N ~ 25
Typical Varian 920-LC UV Vis S/N ~ 38
13
Varian 920-LC Fluorescence Detector
• Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method)
• Time programmable for increase sensitivity• Automatic validation with built-in Hg lamp• Low 12 L dead volume flowcell for improved
chromatography
14
Heated Column Compartment
• Temperature range from ambient + 10 °C up to 65 °C
– Temperature reproducibility +0.15 °C
– Ensures excellent analytical reproducibility
– Uses a low delay volume pre-heater for efficient heat transfer to mobile phase
– Spacious column compartment easily accommodates 2 x 250 mm columns
– Option with electrically actuated column switching valve and injection valve
15
Outstanding Stability
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
11:4
6:00
12:2
2:00
12:5
8:00
13:3
4:00
14:1
0:00
14:4
6:00
15:2
2:00
15:5
8:00
16:3
4:00
17:1
0:00
17:4
6:00
18:2
2:00
18:5
8:00
19:3
4:00
20:1
0:00
20:4
6:00
21:2
2:00
21:5
8:00
22:3
4:00
23:1
0:00
23:4
6:00
0:22
:00
0:58
:00
1:34
:00
2:10
:00
2:46
:00
3:22
:00
3:58
:00
4:34
:00
5:10
:00
5:46
:00
6:22
:00
6:58
:00
7:34
:00
8:10
:00
8:46
:00
9:22
:00
Time
Te
mp
era
ture
(d
eg
ree
s C
)
Mobile phase input Temp
Mobile phase temp inside column
Oven Temp
16
System Reproducibility of 50 Injections
Reproducibility <0.8% over 50 injections and 12 hours
17
Varian 920-LC Software Usability
• User friendly GalaxieTM software
• Simplified, modern, consistent Instrument interface
• Modular 11 pages
• 920-LC 4 pages!!
• One page real-time status and chromatogram
• Advanced integration and calibration
• New IMS capabilities
• Automated instrument validation
• Reduces downtime and operating costs
• Fusion AE
18
Varian 920-LC AIQ (Validation)
AIQ (Analytical Instrument Qualification)• Fully PC controlled IQ and OQ (not PQ)
• Puts the Varian 920-LC at the forefront of regulated capabilities
• Consistent, reproducible methodology• Includes pumps, autosampler, detectors, column
heater
• Chargeable option
• Fully documented
19
Varian 920-LC Ordering Matrix
pump inject detector Oven Detector 2
option
Options
Modular Isocratic 210-LC 10mL Manual 325 PDA, RI, ELSD
Isocratic
00-100890-00Dual piston pump 10 mL head
Auto Dual UV/Vis
Opt FL, RI ELSD
Gradient
00-100891-00Quat, Grad, Degas Auto Dual
UV/VisOpt FL, RI ELSD opt
High Performance Gradient
00-100892-00
Quat, Grad, Degas Auto cooled Dual UV/Vis
Opt FL, RI ELSD opt
Development
00-100893-00Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt
Research
00-100894-00Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt
20
Varian 920-LC Target Markets
• Strong historical position for Varian in analytical
• Varian has the leading range of analytical technologies: spectroscopy, chromatography including consumables, data and services
• Target Users
• Environmental
• Industrial
• Generic Pharmaceutical *
• Academia and Biotech
• Same (but wider) target groups with a stronger product offering
*40% of Regulated Pharma has significant competitive barriers due to installed software base
21
Varian 920-LC Silver Bullets
• Fast LC with superb performance optimised for sub 3 µm columns
– Improved throughput without compromising robustness
• Pulse-free solvent delivery– No need for a pulse damper
• High sensitivity detection• Full system level computer controlled AIQ (validation)
22
Varian 920-LC Integrated Analytical HPLC