Analysis of 18 Polycyclic Aromatic Hydrocarbons using a Varian 920-LC Analytical HPLC and Pursuit ®...
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Transcript of Analysis of 18 Polycyclic Aromatic Hydrocarbons using a Varian 920-LC Analytical HPLC and Pursuit ®...
Analysis of 18 Polycyclic Aromatic Hydrocarbons using a Varian 920-LC Analytical
HPLC and Pursuit® 3 PAH Column
By Phuong TruongVarian Australia Pty Ltd
679 Springvale Rd Mulgrave VIC Australia
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Introduction
• PAH’s are carcinogenic contaminants produced as a by-product of combustion
– Found in drinking water, soils and sludges
• Can be analysed by GC, GC-MS• Compared to GC, HPLC offers
• Easier sample preparation
• Improved sensitivity with FLD
• Better selectivity
• PAH’s are difficult to analyse because of their structural similarity
• Results in long gradient runs of 25 to 30 minutes
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Instrumentation
• Varian 920-LC Analytical HPLC (part no 00100xxx00)• Quaternary gradient pump with 4 channel degasser
• 200 position autosampler with 100 L syringe
• Thermostated column compartment
• Diode Array detector
• Optional Fluorescence detector (part no 99100xxx00)• Varian Pursuit® 3 PAH 100 x 4.6 mm column with
3m packing material (part no A7001100X046)
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Instrument Conditions - Gradient Conditions
Time (min) Flow rate
(ml/min)
% Water % Acetonitrile
0.0 1.3 40 60
3.0 1.3 40 60
7.0 1.3 10 90
13.3 1.3 0 100
16.0 1.3 0 100
16.1 1.3 40 60
21.0 1.3 40 60
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Instrument Conditions - Fluorescence Detector Conditions
Time (min) Excitation
(nm)
Emission
(nm)
0.0 270 323
5.6 250 375
7.1 270 410
8.5 265 380
10.5 280 420
14.7 293 498
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Results
Component UV LOD
(g/L)
Fluoro LOD
(g/L)
USEPA LOD
(g/L)
Naphthalene 2 0.04
Acenaphthylene 5
1-Methylnaphthalene
5 0.05
2-Methylnaphthalene
5 0.06
Acenaphthene 8 0.03
Fluorene 2 0.003
Phenanthrene 0.5 0.04
Anthracene 0.5 0.06
Fluoranthene 1 0.14
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Results
Component UV LOD
(g/L)
Fluoro LOD
(g/L)
USEPA LOD
(g/L)
Pyrene 1 0.02
Benzo(a)anthracene 1 0.01
Chrysene 0.5 0.04
Benzo(b)fluoranthene 1 0.01
Benzo(k)fluoranthene 1 0.01
Benzo(a)pyrene 1 0.01
dibenzo(ah)anthracene
2 0.01
Benzo(ghi)perylene 2 0.01
Indenol(123-cd)pyrene 0.5 0.02
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Conclusion
• The Varian 920-LC and Pursuit 3 PAH column are capable of separating all 18 PAH’s in 15 minutes
• The reproducible gradient delivery and thermostated column compartment ensure excellent retention time reproducibility
• With the optional built-in fluorescence detector, detection limits exceed the requirements of USEPA method 8310
• Offers 30% improvements in throughput compared to alternative HPLC separations (Grace Vydac 201 TP 5 column)