1. Always carry a microscope with one hand on the ________ and the other hand on the _________.

2. A microscope should be stored with the ________ objective in place.

3. Always bring a specimen into focus using the ___________ objective.

4. Never use the ____________ adjustment to focus the high-power objective.

5. Use only the __________ adjustment when focusing with the high-power objective.

6. Lenses should be cleaned with __________.

7.How is the magnification of a compound microscope determined?

NAME LOCATION DESCRIPTION FUNCTION

OCULAR TOP OF MICROSCOPE SINGLE LENS (10X)CAN BE REMOVED

ENLARGES IMAGE OF SPECIMEN FOR VIEWING

BINOCULAR HEAD

BETWEEN OCULAR AND NOSEPIECE

CONTAINS: OCULARSINTERPUPILLARY ADJUSTMENT

INDIVIDUAL EYE FOCUS

SPLITS IMAGE IN TWOALLOWS ADJUSTMENT FOR

USER’S EYES

ROTATING NOSEPIECE

ATTACHED TO UNDERSIDE OF

BINOCULAR HEAD

CHROME WHEEL INTO WHICH OBJECTIVES ARE SET

ALLOWS CHANGING OF OBJECTIVES FOR DIFFERENT POWERS OF MAGNIFICATION

OBJECTIVES ATTACHED TO NOSEPIECE

4 METAL CYLINDERS WITH DIFFERENT MAGNIFYING POWERS (4X-SCANNING, 10X-LOW POWER, 40X-HIGH DRY, 100X-OIL IMMERSION

MAGNIFY IMAGE OF THE SPECIMEN FOR VIEWING

(ALWAYS START WITH 4X OR 10X)

STAGE (PLATFORM)

UNDER THE OBJECTIVES FLAT SURFACE WITH HOLE IN THE CENTER

MICROSCOPE SLIDE IS PLACED UPON THIS SURFACE FOR OBSERVATION

MECHANICAL STAGE

ATTACHED TO STAGE ADJUSTABLE BY KNOBS AT SIDE; CLIP USED TO HOLD AND CONTROL

MOVEMENT OF MICROSCOPE SLIDE

MOVES THE SPECIMEN SLIDE UP AND DOWN, SIDE-TO-SIDE WITH KNOBS AT THE SIDE

SUBSTAGE CONDENSER

UNDER HOLE IN STAGE AN ADJUSTABLE (UP-AND-DOWN) SET OF LENSES (LIKE A “FLAT”

OBJECTIVE)

FOCUSES LIGHT FROM UNDERNEATH THROUGH THE

SPECIMEN FOR VIEWING

IRIS DIAPHRAGM INSIDE THE CONDENSER CAMERA-LIKE OPENING WHICH ADJUSTS WITH SMALL LEVER

REGULATES THE AMOUNT OF LIGHT FOCUSED UPON THE

SPECIMEN

ARM AT BACK (“SPINE” OF THE MICROSCOPE

STRONG SUPPORTING PART OF MICROSCOPE

USED AS A “HANDLE” FOR CARRYING THE MICROSCOPE

BASE(FOOT)

BOTTOM-MOST PART OF THE MICROSCOPE

PART UPON WHICH THE MICROSCOPE SITS

CONTAINS A LIGHT SOURCE AND SWITCH(ES) TO CONTROL THE

BRIGHTNESS

COARSEFOCUS

BOTH SIDES OF ARM NEAR LOWER PART OF

MICROSCOPE

LARGEST KNOBS ON BOTH SIDES OF THE ARM

INITIAL, RAPID FOCUSING OF THE IMAGE OF THE SPECIMEN

FINE FOCUS AT THE TIPS OF THE COARSE FOCUS KNOB

SMALLER KNOBS WHICH TURN MORE EASILY THAN COARSE FOCUS KNOBS

CRITICAL FOCUSING TO SHARPEN THE IMAGE OF THE SPECIMEN

(“FINE-TUNING”)

E. coli

B. cereusS. aureus S. epidermidis