دکتر محسن علیپور 3.doc - pdfMachine from Broadgun Software, http ... ·...

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ƶºƬŬƯ ƱŚººŬƳŻ ƾĪºƃżě ƭƺºƬƗ ƵŚĮºƄƳřŵ ƾºƄƷƹĦě ƾºưƬƗ ƽ ƹŵ ƵŹ ƽ ÏÎ ƵŹŚưƃ ƽ ÕÕ ƽŵ ƹ Źŷō ÎÐÖÏ šŚŰƠƇ ÏÏ Śţ ÐÒ ŚƤƯ ƿ ƶƀ ƽ šřźŧř ƯƺƳŚƳ ǀ ƶƬ ŚƷ ƽ ƀĩř ǀ Ŷ ƲƷō Śŝ Ƃƃƺě ŚƷ ƽ ƵŹƹř ƹ Ƭě ƾ ţř ǀ ƲƬ Ƭĭ ǀ ƩƺĪ źŝ Ż ƿ Ţƀ Ÿě ƿ ź ƽ Ɵ ǀ ƁƺƯ ŢſLjŝƹźŞ źſ ƿ Źƺě ƮſŚƣ ƶ Î ƬƗŶưŰƯ źŤĩŵ ƾ ĭźĪƃ Źřż Ï źŤĩŵ ƣŹ ǀ ƮſŚƣ ƶ Źƺě Ð źƠƘū ŚƋŹŶưŰƯ źŤĩŵ ƽ Ñ ƬƗ ƲƀŰƯ źŤĩŵ ǀ ŹƺĜ Ò ƵŶƴƀƿƺƳ ƽ ƩƹƺƀƯ ƱŚŬƳŻ ƾĪƃżě ƭƺƬƗ ƵŚĮƄƳřŵ ƱŚŬƳŻ ƵŶĪƄƳřŵ ƽ ƾĪƃżě Ƶƹźĭ ƽĥƺƫƺƿżǀƟ [email protected] ŢƟŚƿŹŵ ÎÖ Ð ÖÏ ƁźƿŸě ÏÎ Ò ÖÏ Īģ ǀ ƵŶ ƯŻ ǀ ƝŶƷ ƹ ƶƴ ŚƷŹŚŤųŚſƺƳŚƳ ƵŻƹźƯř ƽ ĩř ƀ ǀ ƶŝ ƲƷō Ŷ ƱřƺƴƗ ƿ ŹĨ ƿ ŸƜƯ ż ƽ ĮƟŶƷ ƹ ƩźŤƴĩ Źŵ ƮƸƯ ǀ ź ƽ ŚºƷŹ ƿ ƳŚºƯŹŵ ŚºƯźĭ ƹŹřŵ Ƃ ƾ ƹ ŴƄºţ ǀ ƹƆ ƱŚƏźſ ƱŚƯŹŵ Ư Źřźƣ ƵŵŚƠŤſř ŵŹƺƯ ƾ ĭ ǀ ŵź ƶƘƫŚƐƯ ƽ ưſ ǀ ƫƺƬſ Ţ ƾ ŚƷŹŚŤųŚſƺƳŚƳ ƽ ƶŝ ŹƺĩŸƯ ƷřŹ ŹƺƔƴƯ ºǀ ŝŚ ƾ ř ƶºŝ ƿ ºƴư ƾ Ʊō ŵźŝŹŚºĩ ř ƝŶºƷ ŚºƷ ºƿ Ʋ ƂƷƹĦě ŵƺŝ ſŹźŝ ƁƹŹ ƾ ƯƺƳŚƳ ǀ ƶƬ ŚƷ ƽ ƀĩř ǀ Ʒō Ŷ ŚƷŻƹŵ Źŵ ƵŹƹř Ƃƃƺě Śŝ Ʋ ƽ ÎÍÍ ƹ ÑÍÍ ƹźƨǀƯ ƾƬǀƯ Źŵ ƭźĭ źŤǀƫ Řţƹźě Śŝ ǀ Ʋ ŚƷ ƽ ƹŚºĭ ƭźſ ƽ ŵźĭ ŭLjºƇř ºƿ Ŷ ưſ ǀ řŢ ƿ Ư ƺƳŚƳ Ʋ ǀ ƶƬ ŚƤƯ Źŵ ŚƷ ƿ ƵŶƄºƳ ŭLjºƇř ƕƺºƳ Śŝ ƶƀ ƽ Ţƀºţ Śºŝ Ʊō Ŭƴſ ǀ Ŷƃ ƵŶ ưſ ǀ ƯƺƳŚƳ Ţ ǀ ƶƬ ě Śŝ ŚƷ Ƃƃƺ PEG Poly Ethylene Glycol Ƴ ǀ ŚƷŻƹŵ Źŵ ż ƽ ƯƺƳŚƳ Śŝ ƶŝŚƄƯ ǀ ƶƬ ŚƷ ƽ Żř ƵŹƹř Śºŝ ƵŶºƃ Źřŵ Ƃƃƺě źƏ ƿ Ţƀţ Ƣ MTT ŚƤƯ ƿ ŵźĭ ƶƀ ƿ Ŷ ƯŚưţ ƾ Ţƀţ ƹŹ źŝ ŚƷ ƽ ƵŵŹ ƽ ƫƺƬſ ƾ LÖÏÖ ƳŚƯŻ ƪƇřƺƟ Źŵ ƹ ƾ ÏÑ ƹ ÑÕ Ŷƃ ƭŚŬƳř ŢƗŚſ ƿ ƶŤƟŚ ŚƷ ƩƺƬſ ŚƷ ƿ ƾ ƯƺƳŚƳ ƉźƘƯ Źŵ ƶĩ ǀ ƶƬ ŚƷ ƽ Źřźƣ ƵŶƃ ŭLjƇř ŶƳŵƺŝ ƶŤƟźĭ ŚƤƯ Źŵ ƿ ƵŶƄƳ ŭLjƇř Śŝ ƶƀ ƽ Ż Ʊō ƿ Ÿºě Ţƀ ƿ ź ƽ ŝ ºǀ źŤƄ ƽ ƯŚºưţ Źŵ ƾ ƱŚƯŻ ƹ ŚƷŻƹŵ ŶƳŵřŵ ƱŚƄƳ ŚƷ ƯƺƳŚƳ ǀ ƶƬ ŚƷ ƽ řŹřŵ ƽ Ƃƃƺě PEG ƱŚƯŻ ƹ ŚƷŻƹŵ ƭŚưţ Źŵ ƫƺƬſ īźƯ ŚƷ ƾ ŝ ǀ źŤƄ ƽ Ʊō ƶŝ ŢŞƀƳ řŹ ŚƷ ƾƿ ƵŹƹř Ƃºƃƺě ƶĩ ŶƳŵřŵ ƱŚƄƳ ŶƴŤƃřŵ ŤƳ ǀ ƶŬ ĭ ǀ ź ƽ ƯƺƳŚƳ ǀ ƶƬ ŚƷ ƽ ƀƳ ƵŶƄƳ ŭLjƇř ưſ ƵŶƃ ŭLjƇř ƕƺƳ ƶŝ ŢŞ ǀ ŝŢ ǀ źŤƄ ƽ ƶĩ ŶƳŵřŵ ƱŚƄƳ LJŚưŤůř ƃŚƳ ƾ ĪƄºţ Żř ǀ ƪ ºƿ ƶºƤƬů Ĩ ƽ Řţƹźě ǀ ºƴ ƾ ƱřƺƴƗ ŢŰţ Hard Corona ř Źƹŵ ƶŝ ƿ ƯƺƳŚƳ Ʋ ǀ ƶƬ ŰƯ Źŵ ŚƷ ǀ ƹŚů Ǝ ƽ Řţƹźě ǀ ĪƄţ ƭŶƗ ƹ Ʋ ǀ ƵŶƃ ŭLjƇř ƕƺƳ Źŵ Ʊō ƪ ƾƯ ŶƃŚŝ ř źŝ ƵƹLjƗ ƿ ƂƷŚĩ Ʋ ƫƺƬſ īźƯ ƾ ƯƺƳŚƳ Ǝſƺţ ǀ ƶƬ ŚƷ ƽ řŹřŵ ƽ ƵŹƹř Ƃƃƺě ŚƤƯ Źŵ ƿ Śŝ ƶƀ PEG ŝ ǀ ŧŚţ źĮƳŚ ǀ ƵŻřŶƳř ƶŝ ƶūƺţ Śŝ Ƃƃƺě ƕƺƳ ź ƽ ưſ źŝ šřŹŷ ǀ ƫƺƬſ Ţ ƾ Ʊō ŚºƷ Ţſř ĥřƹ ƱŚĭ Ƭĩ ǀ Ŷ ƽ ƯƺƳŚƳ ǀ ƶƬ ŚƷ ƽ ƀĩř ǀ Ƃƃƺě ƵŹƹř Ƃƃƺě ƲƷō Ŷ PEG MTT Ż ƿ Ÿě Ţƀ ƿ ź ƽ ƵŵŹ ƽ ƫƺƬſ ƾ LÖÏÖ ƶƯŶƤƯ ƵŻƹźƯř Źŵ ƽŹƹŚºƴƟ ƺƳŚºƳ ƵŻƺºů ƽ Żř ƾĪºƃżě ƹ ƽĥƺºƫƺǀŝ Źŵ ƶƬưū ƹŹřŵ ƂƿŚƷŹ ƬƯŚů ǀ ƹŹřŵ Ʋ ƽŻŚºſřŶū ŢºƟŚŝ ƮǀƯźţ ƩƺƬſ ƲǀŘţƹźě ƹ ŚƷ žƳŚºƳŻŹ ƽŹřŵźŝźƿƺƈºţ ƹ ƾƳŚƯŹŵ ŚƯźĭ ŚƷ ƱŚƏźººſƱŚººƯŹŵ ƹƆǀŴƄººţ ƾƀººǀƏŚƴƜƯ Ï ƹ Î ƲǀººƴĤưƷ Î ƽĥƺƫƺƿżǀƟ ŶƃŹř ƾſŚƴƃŹŚĩ ƽƺŬƄƳřŵ ƽĥƺƫƺƿżǀƟ Ƶƹźĭ ƱŚŬƳŻ ƾĪƃżě ƭƺƬƗ ƵŚĮƄƳřŵ Ï ƵŵŹƹōźƟ ƾƈƈŴţ ƽřźŤĩŵ ĥƺƫƺǀŝ ƽŚƷ ƫƺƬſ ĨƳŚŝ Ʊřźƿř ŹƺŤſŚě ƺŤǀŤƀƳř ŵŚŤſř Ĩƿ ƾ Ʊřźƿř Ð ƈƈŴţ ƽřźŤĩŵ ƾ ŹŚƿŵŚŤſř ƽŹƹŚƴƟ ƺƳŚƳ ƵŚĮƄƳřŵ ƱřźƸţ Ñ ƈƈŴţ ƽřźŤĩŵ ƽĥƺƫƺĩŚƯŹŚƟ ƾ ŹŚǀƄƳřŵ ƱŚŬƳŻ ƾĪƃżě ƭƺƬƗ ƵŚĮƄƳřŵ Ò ƽĥƺƫƺƿżǀƟ ƾƈƈŴţ ƽřźŤĩŵ ŹŚǀƄƳřŵ ƱŚŬƳŻ ƾĪƃżě ƭƺƬƗ ƵŚĮƄƳřŵ 3- (4 ,5 -D imethylthiazol -2 -yl) -2, 5- diphenyltetrazolium bromide (MTT)

Transcript of دکتر محسن علیپور 3.doc - pdfMachine from Broadgun Software, http ... ·...

Page 1: دکتر محسن علیپور 3.doc - pdfMachine from Broadgun Software, http ... · ƱŚººŬƳŻ ƾĪºƃżě ƭƺºƬƗ ƵŚĮºƄƳřŵ ƾºƄƷƹĦě ƾºưƬƗ ƽƶ ºƬŬƯ

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35

The Effect of Urea and Polyethylene Glycol Coated Iron Oxide Nanorods on Viability of

Mouse Fibroblasts

Ghasempour S1, Shokrgozar MA2, Ghasempour R3, Jafari MR1, Alipour M1 1Dept. of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

2Pasteur Institute of Iran, National Cell Bank, Tehran, Iran 3Dept. of Renewable Energy of Environment Engineering, Faculty of New Sciences of Technology, University of Tehran, Tehran, Iran

Corresponding Author: Alipour M, Dept. of Physiology & Pharmacology, School of Medicine, Zanjan University of

Medical Sciences, Zanjan, Iran

E-mail: [email protected]

Received: 9 Jun 2013 Accepted: 21 Aug 2013

Background and Objective: Currently, as an important trace element, is increasingly used in drug delivery,

hyperthermia and diagnosis and treatment of cancer. The main objective of this study was to determine the

cytotoxicity effects of nanostructure iron oxide and to implement the findings for its safe applications.

Materials and Methods: Urea coated iron oxide nanorods were modified with fetal bovine serum� Toxicity

at doses of 100 and 400ìg/ml in modified nanorods vs. non-modified nanorods was tested via (3-(4, 5-

dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)�assay. Toxicity of Polyethylene glycol (PEG)

coated iron oxide nanorods with identical doses were compared with Urea coated iron oxide nanorods by

MTT assay. All the procedures were carried out on L929 cell lines for periods of 24 and 48 h.

Results: Cells on exposure to modified nanorods had more viability than non-modified ones at all doses and

all the time points. PEG coated in comparison to urea coated nanorods exhibiteda higher cell death at all

doses and all time points.

Conclusion: Based on the obtained results, unmodified nanorods in comparison to modified ones

demonstrated more toxicity which appears to stem from formation of a protein ring called Hard Corona

around these nanorods in a protein contained media. Protein rings are not formed in the modified nanorods.

Meanwhile, the reduction of apoptosis in urea coated nanorods as compared with PEG, verifies that the type

of coating depending on size is effective on cellular toxicity.

Keywords: Iron oxide nanorods, MTT, Viability, Urea coated, L929 cell

��