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www.pan-biotech.com Page 3
Overview
1. SerumIntroductionCertificate of SuitabilityBovine SerumFBS Good Product FamilySpecial Fetal Bovine SerumCertificate of AnalysisTreated SerumOther Animal SerumHuman SerumSerum Services
2 - 133
3 - 45
5 - 67
8 - 910 - 11
1112
12 - 132. Serum-free Systems
Introduction & Product NumbersSerum SubstitutesSerum-free Media
14 - 3515 - 1718 - 2021 - 35
3. MediaIntroductionCell Culture MediaCell Specific Media
36 - 7737
38 - 7172 - 77
4. Serum-free Stem Cell MediaIntroductionStem Cell Media
78 - 9179
80 - 915. Reagents
Media SupplementsBuffered Salt SolutionAmino Acids and VitaminsAntibiotics and Antifungal DrugsEnzymes for Cell DissociationAttachment FactorsSeperating SolutionsSeparating Solutions Pre-FilledPAN SL-S Product LineCryo PreservationDisinfectants
92 - 11393
94 - 9697
98 - 99100 - 101102 - 104
105106107108
109 - 1136. Biologicals
IntroductionHuman Cytokines and Growth FactorsOther Species Cytokines and Growth FactorsChemokines
114 - 121115
116 - 118119120
7. Molecular BiologyPolymerasesMolecular Biology Reagents
122 - 133123 - 128129 - 133
8. PANsysIntroductionPANsys 3000
134 - 136135
135 - 136
Dear valued customer
We think that the current biotech industry favors experienced, mid-sized and flexible pro-ducers with a broad product portfolio, strong research and development capabilities, best-in-class quality, strong business partner relationships and a wide geographic reach. During the last 26 years PAN-Biotech demonstrated its strength with innovative, first-class quality products and excellent services for cell culture around the globe.
For you, as users and customers of cell culture products, the supply situation has changed rapidly in first quarter of 2013. Due to several mergers and acquisitions of bigger as well as smaller companies there were frictions and changes in supply chains. The customer prob-lems and frustrations were highlighted with the question(s):Can you deliver / support us / rescue our production...? YES, WE CAN !
We can supply FBS, media, reagents and all other products presented in this catalogueWe can support you We can develop / optimise your media and much more
Our market prediction for 2013 was more frictions and turbulences. Some of the recent ac-quisitions went through the same route: logistic changes, lack of customer responsiveness, delivery problems, price increases, intransparency and so on. The overall results for custo-mers was a limited choice of vendors and huge international conglomerates with reduced customer service.
PAN-Biotech still remains as one of a few privately owned, ownership managed companies. We value our customers, we value their needs and requirements, we value product quality, we value openness and transparency, we value service and commitments, we value trust and partnership. We followed along these values for the last 26 years and we intend to keep these values for the next 26 years.
We thank all our existing customers, our dedicated and highly motivated staff and our sup-pliers and industry partners who made possible
26 years biotech made in Germany by PAN-Biotech
And we cordially welcome all our new customers, OEM partners and interested internati-onal distributors as well as new business partners. We guarantee you the values described above and our personal commitment for excellent products and services.
Yours sincerely
Christian Niewolik & Jens HartmannOwners & Managing Directors
March, 2014PAN-Biotech GmbH
Phone: +49 (0)8543/6016-30 Fax: +49 (0)8543/6016-49www.pan-biotech.de www.pan-biotech.com
Introduction
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Serum
The function of serum in cell cultures• Stimulates cell growth, proliferation and differentia-
tion through hormonal factors• Adhesion factors facilitate and enhance cell attach-
ment on culture dishes (bio-matrix)• Transport and binding proteins provide hormones,
minerals and lipids• Inhibition of toxic substances by binding to serum
proteins
Animal serumThe dose of serum added to a cell culture as nutrient sour-ce depends on factors such as cell type, primary culture or cell line, adherent or suspension culture, and usually is in the range of 5 % to 15 % of the total liquid volume and most times used at 10 %.
Serum is produced from animal blood and fetal bovine serum is the most widely used because it contains an especially high amount of growth factors due to its ori-gin – the blood of fetuses is a by-product of slaughtered cattle.
Advantages of PAN-Biotech• Own raw material resources in different coun-
tries: Australia, South Africa, South America (Brazil) and the United States of America (USA)
• Certificate of Suitability (COS) no. R1-CEP 2002-167-Rev 00
• Licensed according to the EU-decree no. 1774/2002 with vet. no. DE 09 275 0001 14
• Every single batch is fully documented – from the country of origin to the end product
• Every process, from collection of raw serum to pro-duction, is specified in standard operating instruc-tions (SOP) which will be provided upon request
• We offer special types of serum: charcoal absor-bed, delipidized, dialyzed, gamma irradiated, heat inactivated and gamma globulin reduced
• Highest production and safety standards for serum manufacturing
• Best references from industry and research• Extensive analyses and tests are presented in Certifi-
cate of Analysis (CoA)
We exclusively use serum from guaranteed BSE-free collection areas. In addition, we can also confirm that South Africa, as a country of origin, is free from scrapie. No serum batches from Great Britain as the country of origin are processed by PAN-Biotech. We warrant the submission of a complete documentation, consisting of a certificate of origin and a veterinary certificate, shipping documents and a certificate of analysis. Furthermore, every single procedure during an individual production process is documented and then summarized in a pro-duction protocol.
Certificate of Suitability
DeclarationThe manufacturing process and quality control testing are performed in accordance with the submitted records and with a suitable quality assurance system in compli-ance with ISO 9001 quality standards. This quality as-surance system verifies traceability and batch consistency. PAN-Biotech conducts internal and external audits for its quality system on an annual basis. In addition, PAN-Bio-tech audits its raw material serum suppliers on a cyclical basis and reviews the facilities, manufacturing processes and documentation for the collection, handling, storage and transport of raw serum. PAN-Biotech is willing to be inspected, in accordance with the relevant legislation, on request of a relevant authority before and/or after being granted a certificate of suitability.
Production site: PAN-Biotech GmbHAm Gewerbepark 1394501 Aidenbach / GERMANY
Serum Hotline: +49(0)8543/6016-55
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Chapter 1
Serum
Introduction 3Certificate of Suitability 3-4Bovine Serum 5FBS Good Product Family 5-6Special Fetal Bovine Serum 7Certificate of Analysis 8-9Treated Serum 10-11Other Animal Serum 11Human Serum 12Serum Services 12-13
1 1
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Serum
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Serum
1 1Bovine Serum
Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse
and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany. All serum lots are virus and mycoplasma tested.
Fetal Bovine Serum, Australia origin 100 ml500 ml
P30-1301P30-1302
Fetal Bovine Serum, South Africa origin 100 ml500 ml
P30-1505P30-1506
Fetal Bovine Serum, South America origin 100 ml500 ml
P30-3305P30-3306
Fetal Bovine Serum, US admissible 100 ml500 ml
P30-1701P30-1702
Fetal Bovine Serum, US origin 100 ml500 ml
P30-1401P30-1402
Fetal Bovine Serum Premium, South Africa origin 100 ml500 ml
P30-1501P30-1502
Fetal Bovine Serum Premium, South America origin 100 ml500 ml
P30-3301P30-3302
Sera Pro, Fetal bovine serum, low Endotoxin, EU approved
100 ml500 ml
P30-5100P30-5500
FBS EU Professional, Filtrated bovine serum, EU approved
100 ml500 ml
P30-8100P30-8500
Bovine Serum, variable origins 100 ml500 ml
P30-0601P30-0602
Calf serum - newborn, variable origins 100 ml500 ml
P30-0401P30-0402
FBS Good Product Family
The FBS Good product family contains specially processed serum products. Serum of selected batches is filtrated and separated into individual components by a sophisticated chromatographic method. The growth promoting components contained in the serum are then combined and restored in a defined process. Compared to conventional fetal bovine serum the FBS Good product family has been shown to support and promote cell growth of many different cell types equally well or even better.
Advantages
• Innovative new products• Minor batch to batch variation• Once tested – always same quality• No batch testing required• No lot reservation required
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Serum
FBS Good Product Family1
By developing FBS Good Biotech we wanted to create a naturally defined serum with a sustained growth promoting property and a higher safety. It is tested according to EMA (also known as EMEA) and Ph. Eur. guidelines for use in the bio-pharmaceutical industry. FBS Good Biotech only contains serum of highest quality from defined countries as specified. It is not blended or enhanced by addition of growth factors or proteins.
FBS Good Biotech advantages
• Tested according to EMEA CPMP/BWP/1793/02• Tested according to Ph. Eur. 01/2008:2262• Very low endotoxin level• Suitable for bio-pharmaceutical processing• Expanded quality control
By developing FBS Good Forte we wanted to create a naturally defined serum with an increased growth promoting property and a higher safety. Therefore, additional growth fortifying compounds have been added to increase cell proliferation. FBS Good Forte only contains serum of highest quality from defined countries as specified. In addition, growth promoting and stabilizing compounds (e.g. proteins, salts, sugars, vitamins) have been added to further enhance the stability of the serum as well as the proliferation of many different cell types.
FBS Good Forte advantages
• Reproducible enhanced growth properties• Very low endotoxin level• Suitable for many different cell types• Continued high quality• No more batch testing required
Fetal Bovine Serum Biotech
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Serum
1FBS Good, Filtrated bovine serum, Australia origin 100 ml
500 mlP40-39100P40-39500
FBS Good, Filtrated bovine serum, EU approved 100 ml500 ml
P40-37100P40-37500
FBS Good, Filtrated bovine serum, US origin 100 ml500 ml
P40-38100P40-38500
FBS Good Forte, Filtrated bovine serum with Additive Fortifier, Australia origin
100 ml500 ml
P40-49100P40-49500
FBS Good Forte, Filtrated bovine serum with Additive Fortifier, EU approved
100 ml500 ml
P40-47100P40-47500
FBS Good Forte, Filtrated bovine serum with Additive Fortifier, US origin
100 ml500 ml
P40-48100P40-48500
FBS Good Biotech, Filtrated bovine serum, Australia origin, tested acc. EMEA 1793 and Ph. Eur. 2262*
100 ml500 ml
P40-59100P40-59500
FBS Good Biotech, Filtrated bovine serum, US origin, tested acc. EMEA 1793 and Ph. Eur. 2262*
100 ml500 ml
P40-58100P40-58500
*tested upon request and produced after receipt of order
FBS GoodBy developing FBS Good we wanted to create a naturally defined serum with a sustained growth promoting property and a higher safety. FBS Good only contains serum of highest quality from defined countries as specified. It is not blended or enhanced by addition of growth factors or proteins.
FBS Good advantages
• Reproducible growth properties• Very low endotoxin level• Suitable for a great variety of cells• Continuous quality control
FBS Good Forte FBS Good Biotech
Special Fetal Bovine Serum
Pansera ES, Fetal bovine serum, Australia origin, special designed for embryonic stem cells
100 ml500 ml
P30-2605P30-2606
Pansera ES, Fetal bovine serum, EU approved,special desgined for embryonic stem cells
100 ml500 ml
P30-2601P30-2602
Pansera ES, Fetal bovine serum, US origin, special designed for embryonic stem cells
100 ml500 ml
P30-2608P30-2609
Fetal Bovine Serum Biotech, Australia origin, tested acc. EMEA 1793 and Ph. Eur. 2262*
100 ml500 ml
P40-1301P40-1302
Fetal Bovine Serum Biotech, US origin, tested acc. EMEA 1793 and Ph. Euro. 2262*
100 ml500 ml
P40-1401P40-1402
*tested upon request and produced after receipt of order
Pansera ES
Our specially developed, proprietary processing methodology for serum enables us to offer a special fetal bovine serum for embryonic stem cells (ES).
Advantages
• Reproducible constant growth properties• Improved cloning efficiency• More undifferentiated clones• Permanent strict quality control• No need for further testing of different batches
The bio-pharmaceutical industry is facing a constantly growing demand for high quality, extensively tested fetal bovine serum originating from FDA-approved regions. PAN-Biotech is meeting this demand and offers a new product, Fetal Bovine Serum Biotech, which is tested according to EMA (also known as EMEA) and Ph. Eur. guidelines.
Fetal Bovine Serum Biotech originates exclusively from Australia or the USA, both of which are approved regions by FDA´s CFR. The entire production process is followed by close inspections and quality controls – from the collection of raw material to the final production and sterile filtration procedure, all steps are documented and traceable.
Especially a possible contamination with bovine viruses has to be excluded. Therefore, a multitude of tests is performed to meet highest safety requirements.
This product is tested according to EMEA CPMP/BWP/1793/02 and Ph. Eur. 01/2008:2262 upon request. In addition, EMA/410/01 rev. 3 and EMA/CHMP/ BWP/457920/2012 rev. 1 are also included as guidelines for testing procedures. Besides extensive testing for viral contamination, supplementary sterility testing is performed before, during and after filling of the product.
ApplicationFetal Bovine Serum Biotech is particularly suited for the production of virus, vaccine, monoclonal antibodies, recombinant protein and growth factors, as well as the manufacture of other bio-pharmaceutical products.
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Serum
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Serum
1 1Treated Serum
Fetal Bovine Serum, activated charcoal absorbed, EU approved
100 ml500 ml
P30-2301P30-2302
Fetal Bovine Serum, delipidized, EU approved 100 ml500 ml
P30-3401P30-3402
Fetal Bovine Serum, dialyzed, EU approved 100 ml500 ml
P30-2101P30-2102
Fetal Bovine Serum, gamma irradiated, US origin 100 ml500 ml
P30-2008P30-2009
FBS EU Professional, gamma irradiated, EU approved 100 ml500 ml
P30-2081P30-2085
Fetal Bovine Serum, heat inactivated, Australia origin 100 ml500 ml
P30-1905P30-1906
Fetal Bovine Serum, heat inactivated, US origin 100 ml500 ml
P30-1908P30-1909
FBS EU Professional, heat inactivated, EU approved 100 ml500 ml
P30-1981P30-1985
Ultra low IgG, Fetal bovine serum, EU approved 100 ml500 ml
P30-2801P30-2802
Tetracycline-free serum, Fetal bovine serum,EU approved
100 ml500 ml
P30-3601P30-3602
Activated charcoal treated serum Dialyzed serum
Fetal bovine serum is heated in a water bath with dextran and activated charcoal. The activated charcoal, together with the substances bound to it, is then removed by centrifugation and filtration.
Application
• Work involving reduced hormone content (steroids)• Work involving reduced growth factors (prevention
of cell differentiation)• Receptor studies (e. g. estrogens)• Minimizes lot-to-lot variations in serum
Serum is dialyzed with a 10,000 Dalton exclusion membrane against physiological saline solution (alternative DPBS) until the glucose content is below 10 mg/100 ml.
Application
• Radioactive labeling studies• Hormone-free applications• Tests intolerant for small molecules such as
nucleotides (hypoxanthine, thymidine), amino acids (serine, alanine etc.), sugars or metabolites
Delipidized serum
Lipids are removed from serum by affinity chromatography.
Application
• Lipid metabolism studies• Arteriosclerosis research
Gamma irradiated serum
Serum is exposed to irradiation > 25 kGy
Application
• Biopharmaceutical production• Virus production• Vaccine production• Manufacturing of diagnostic products
Heat inactivated serum Ultra low IgG serumThe average IgG level in fetal bovine serum is in the range of 70 to 330 µg/ml. The IgG content in our Ultra low IgG serum is reduced by affinity chromatography (protein-G affinity column) to max. 5 µg/ml. The biological activity of serum is not affected.
Application
• Antibody production• Monoclonal antibodies• Radioactive labeling
Serum is heated for 30 min to 56 °C in a water bath under repeated gentle mixing.
Application
• Measurements of lactate dehydrogenase in the culture supernatant as a marker for cell damage (serum LDH is inactivated by heat)
• Minimizes lot-to-lot variations in serum (all thermo-labile components are removed)
• Studies on vitamins and growth factors• Enhance viral safety, since heat-labile viruses are
inactivated• Tests that do not tolerate presence of complement
(complement destruction) Tetracycline-free serum
Fetal bovine serum is tested for absence of tetracycline using the TET-off system (luciferase).
Application
• TET-on / TET-off regulated gene expression• Transfections• Expression studies
Chicken serum 100 ml500 ml
P30-0301P30-0302
Donkey serum 100 ml500 ml
P30-0101P30-0102
Goat serum 100 ml500 ml
P30-1001P30-1002
Hamster serum 10 ml P30-0210
Horse serum 100 ml500 ml
P30-0701P30-0702
Lamb serum 100 ml500 ml
P30-0801P30-0802
Mouse serum 10 ml100 ml
P30-0200P30-0201
Pig serum 100 ml500 ml
P30-0901P30-0902
Rabbit serum 100 ml500 ml
P30-1101P30-1102
Rat serum 10 ml100 ml
P30-01901P30-01901E
Sheep serum 100 ml500 ml
P30-4101P30-4102
Other Animal Serum
All serum tested for virus and mycoplasma. Other serum upon request.
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Serum
Serum testing
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Serum
Human serum is manufactured from human plasma by addition of calcium chloride. This results in clotting of the plasma. After removing the clot, the human serum is washed and concentrated by ultra-filtration and finally filtered through a combination of depth- and membrane- filters.
Human Serum1 1Off-the-clot serum (True human serum)Off-the-clot serum is prepared from human whole blood collected without anti-coagulant, allowed to clot at room temperature and then centrifuged to remove the clot. We provide single donor units as well as pooled off-the-clot serum. Off-the-clot serum is filtered through depth and membrane filters before filling.
Human serum 100 ml500 ml
P30-2401P30-2402
Human AB serum 100 ml500 ml
P30-2501P30-2502
Human AB serum (male) 100 ml500 ml
P30-2901P30-2902
Human serum off-the-clot 100 ml500 ml
P30-2701P30-2702
Serum Services
PAN-Biotech offers a variety of services and test procedu-res for your serum. We deliver these services fast and cost efficient, using the latest up-to-date techniques.
Profit from our expertise! If you need further special tes-ting or particular services please contact PAN-Biotech. In most cases we can find a solution.
Special processing of serum lots
Treatment Method
Charcoal absorbedSerum is heated in a water bath with dextran and activated charco-al. The activated charcoal, together with the substances bound to it, is then removed by centrifugation and filtration.
Delipidized Lipids are removed from serum by affinity chromatography
Dialyzed Serum is dialyzed with a 10,000 Dalton exclusion membrane against physiological saline solution
Heat inactivated Serum is heated for 30 min to 56 °C in a water bath under repeated gentle mixing
IgG reduced The IgG content in serum is reduced by affinity chromatography to max. 5 µg/ml. The biological activity of serum is not affected.
Sterile filtered Serum passes a series of filters with decreasing pore sizes. The last filtration step is done with a 0.2 µm pore size sterile filter.
Test MethodAlbumin, Globulins Serum protein electrophoresis
Bacterial count
Detection of total number of viable aerobic germs will be either done by membrane filtration or plate-flush-method or as surface method. The microorganisms are detected as colony forming units per ml (CFU/ml) on Caso agar plates.
Cell growth Growth test of murine myeloma cells (SP2/0-Ag14) and murine fibroblasts (L929)
Cholesterol Colorimetric test (CHOD-PAP)
Cloning efficiency
Murine myeloma cells (SP2/0-Ag14) are plated on microtiter pla-tes (one cell per well). After 7 days of incubation the developed cell colonies are counted (= absolute cloning efficiency). The results are normalized to a previously tested reference serum (= relative cloning efficiency).
Endotoxin Kinetic limulus amoebocyte lysate test (LAL)Glucose Colorimetric test (Trinder reaction)Hemoglobin Determined spectrophotometrically at three different wave lengthsIgG Radial immune diffusion
MycoplasmaThree different detection systems are used: DNA-binding fluore-scence dye (DAPI), microscopic analysis of microbial cultures and test kits which detect mycoplasma specific enzymes
Osmolality Analyzed by freezing point depressionpH value Measured with pH-electrode
Plating efficiency
Murine fibroblasts (L929) are plated into a Petri dish. After 14 days of incubation the fixed cell colonies are stained with Giemsa and counted (= absolute plating efficiency). The results are normalized to a previously tested reference serum (= relative plating efficien-cy).
SterilityThe absence of bacterial or fungal contamination is verified by dual incubation with Caso-Bouillon or Thioglycolat-Bouillon ac-cording to Ph. Eur. at 32 °C and 20 °C
TetracyclineTested by a TET-off system of a CHO-luc cell line. Absence of tetracyline induces expression of luciferase, which is quantified using the luciferase test system from Promega.
Total protein Colorimetric test (Biuret reaction)
Triglycerides Colorimetric test (Trinder reaction)
Virus testingThe following viruses and the presence of their antibodies are tes-ted by cytopathic effect: Bovine viral diarrhoea virus (BVDV), bo-vine herpes virus (BHV 1) and parainfluenza virus (PI-3)
Virus testing according EMEA guidelinesThe following virus tests are performed according to EMEA guideline CPMP/BWP/1793/02:• Bluetongue and related orbi viruses• Bovine adenovirus• Bovine parvovirus
• Bovine respiratory syncytial virus (BRSV)• Bovine viral diarrhoea virus (BVDV)• Rabies virus (rabies)• Reo virus• Bovine polyoma virus (BPyV)
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MediaChapter 3
Introduction
You only want highest quality media for cell cultivation?
At PAN-Biotech, perfect raw materials combined with state-of-the-art technologies guarantee a first-class quality of our media.
Water is the most important component of liquid media, which is why water purity is of outstanding importance for the quality of media. The water we use generally has a very low endotoxin level of < 0.005 EU/ml, and therefore is of highest purity.
Our media are placed in quarantine until quality control procedures are finished. This guarantees an excellent quality of the final product.
Advantages of our cell culture media
• Raw materials used are tested according to the highest possible quality standards
• Standard filling in sterile, high-class PET bottles• Batches of 10 litre up to 4000 litre• Custom service of product optimisation and further
development for specific applications and purposes• CE-label according to medical product law available
upon request
Other sizes and custom formulation
Almost all media available from PAN-Biotech can be filled in special containers as per customer requirement. Besides standard bottles in 100, 500, and 1000 ml, medium can be filled in cans (up to 10 L), bags (up to 500 L), or other containers with fittings according to customer specifications for special applications such as continous feed process or production purposes.
Delivery time
Standard media:In principle within 3 working days in Germany; otherwise we will inform you.Special media and custom products:Within Germany in 4 to 6 weeks after receipt of order.
Shelf life
Powder media 2 yearsLiquid media without Glutamine 2 yearsLiquid media with stable Glutamine 2 yearsLiquid media with L-Glutamine 1 year
Liquid media with L-Glutamine can be used also after the expiry date, but have to be supplemented with new L-Glutamine in this case. Shelf life starts on date of production!
Storage
Powder media 2 – 8° CLiquid media 2 – 8° C protected from light
Benefit from the experience and know-how of PAN-Biotech. Our state-of-the-art production facilities, with a production line specifically installed for these requirements, allow us to produce the formulations especially developed for your needs in constant high quality also for longer periods of time, and to make batch sizes adapted to your need. Our team of scientists will be pleased to advise you regarding your proprietary formulation.
For further information regarding the dependency of ph-values in media on the CO2 concentration in the incubator please refer to our website at www.pan-biotech.com.
3
MediaIntroduction 37Alpha MEM 38-39BME with Hank´s Salts 40BME with Earle´s Salts 40CMRL-1066 Medium 41Dulbecco´s Modified Eagle Medium 42-45DMEM / F12 46-47Glasgow MEM (BHK21) 48Grace´s Insect Medium 49Ham´s F10 Medium 50Ham´s F12 Medium 51Iscove´s Modified Dulbecco´s Medium 52-53IPL-41 Insect Medium 54Joklik-MEM 55Leibovitz´s L-15 Medium 56MC Coy´s 5A Medium 57MCDB Medium 58Medium 199 with Earle´s Salts 59Medium 199 with Hank´s Salts 60MEM with Earle´s Salts 61-62MEM with Hank´s Salts 63RPMI 1640 64-66Schneider´s Drosophila Medium 67TC 100 Insect Medium 68TNM-FH Medium 69Waymouth´s MB 752/1 Medium 70William´s Medium E 71Endopan 3 72Endopan MV 72Endopan PRO 73Pantum 74Hepatopan 74Melanopan 74Neuropan 75Stempan 75EMEM Fibroblasts 75Amniopan 76Amniopan S2 76Marrowpan 77Marrowpan S2 77
3
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Media
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Media
Alpha MEMComposition
Components mg/LInorganic Salts
Calcium chloride x 2H2OMagnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O
264.92139.52400.00
6,800.00140.00
OtherCompo-nents
D(+)-Glucose anhydrousHepesLipoic acidPhenol redSodium pyruvate
1,000.005,958.00
0.2010.00
110.00AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
25.00126.64
50.0030.00
100.0024.00
292.0075.0050.0042.0052.4052.4072.4715.0032.0040.0025.0048.0010.0036.2046.00
Vitamins L-Ascorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HClVitamine B12
50.000.101.001.001.002.001.001.000.101.001.33
Ribonu-cleosides
AdenosineCytidineGuanosineUridine
10.0010.0010.0010.00
Deoxy-ribonu-cleosides
2’-Deoxyadenosine x H2O2’-Deoxycytidine x HCl2’-Deoxyguanosine2’-Deoxythymidine
10.0011.0010.0010.00
Powder Media
Alpha MEM Eagle(1) 10 L P03-2410without L-Glutamine 50 L P03-2450with Ribonucleosideswith Deoxyribonucleosides without NaHCO3 Alpha MEM Eagle(1) 10 L P03-2510with L-Glutamine 50 L P03-2550with Ribonucleosideswith Deoxyribonucleosides without NaHCO3
Alpha MEM Eagle(1) 10 L P03-2310with L-Glutamine 50 L P03-2350without Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3
Alpha MEM Eagle(1) 10 L P03-2610with L-Glutamine 50 L P03-2650with 25 mM Hepes with Ribonucleosideswith Deoxyribonucleosideswithout NaHCO3
Alpha MEMDescriptionAlpha MEM is a different formulation of MEM Eagle and contains a higher concentration of amino acids. It also has a higher concentration of lipoic acid, vitamins and pyruvate. Primarily it was developed for the cultivation of hamster kidney cells, but today it is used for a broad range of mammalian cells. Among others the alpha MEM promotes the growth and progeny of bone marrow cells in suspension culture and monolayer. A further possibility is the use as a separation medium or for the out-breeding of amniotic cells.
Liquid Media
Alpha MEM Eagle(1)
without L-Glutaminewithout Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21050 Alpha MEM Eagle(1)
with L-Glutamine with Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21500
Alpha MEM Eagle(1)
with stab. Glutaminwith Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21250
Special Media
Alpha MEM Eagle(2)
without L-Glutaminewith Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21150
Alpha MEM Eagle(2)
with L-Glutaminewithout Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21060
Alpha MEM Eagle (2)
with stab. Glutaminewithout Ribonucleosidswithout Deoxyribonucleosidswith 2.2 g/L NaHCO3 500 ml P04-21350
Alpha MEM Eagle (2)
with L-Glutaminewithout Glucosewith Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21502
Alpha MEM Eagle (2)
without Glutaminewithout Phenol redwithout Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21051
3 3
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Media
CMRL-1066 MediumCompositionDescription
The CMRL is a nucleosid- and vitamin-rich medium. In the past it was developed to clone monkey-kidney cells and as long time culture medium for L-cells. It is suitable for many types of human and monkey cells and also for other mammalian cells, especially by using horse and calf serum.
Liquid Media
CMRL – 1066(1)
without L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-84600
Special Media
CMRL – 1066(2)
with L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-84500
Media
Page 42 www.pan-biotech.com Page 43
Media
Components mg/LInorganic Salts
Calcium chloride x 2H2OPotassium chlorideMagnesium sulfate driedSodium acetate anhydrousSodium chloridedi-Sodium hydrogen phosphate x H2O
264.92400.00139.55
50.006,799.00
139.75
OtherCompo-nents
CholesterolD(+)-Glucose anhydrousGlutathione (red.)Sodium glucuronate x H2OTween 80
0.201,000.00
10.004.005.00
Coenzy-me
Cocarboxylase x HClCoenzyme A Trilithiumsalt x 2H2ONADNADP sodium saltUTP
1.002.60
7.001.001.00
AminoAcids
L-AlanineL-Arginine x HClL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
25.0070.0030.00
260.0020.00
100.0075.0050.0020.0010.0020.0060.0070.0015.0025.0040.0025.0030.0010.0040.0025.00
Vitamins L-Ascorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HClVitamine B12
50.000.101.001.001.002.001.001.000.101.001.33
Deoxy-ribonu-cleosides
2’-Deoxyadenosine x H2O2’-Deoxycytidine x HCl2’-Deoxyguanosine2’-Deoxythymidine
10.0011.0010.0010.00
BME with Earle´s SaltsDescriptionIn the fifties of the last century it was found that mammalian cells do not only need the 10 essential amino acids, but also cystine, tyrosine and glutamine. In addition to these three amino acids BME includes
When 5,958.00 mg/L HEPES is included there is only 6,300.00 mg/L sodium chloride.
Composition
Liquid Media
BME with HBSS(1)
without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-26050
When 5,958.00 mg/L HEPES is included there is only 7,500.00 mg/L sodium chloride. Liquid Media
BME with EBSS(1)
without L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-25050
Special Media
BME with EBSS(2)
with L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-25500
BME with Hank´s Salts
Components mg/LInorganic Salts
Calcium chloride x 2H2O Magnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O
264.92139.52400.00
6,800.00140.00
OtherCompo-nents
D(+)-Glucose anhydrousHepesPhenol red
1,000.005,958.00
10.00AminoAcids
L-Arginine x HClL-CystineL-GlutamineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine
21.0012.00
292.008.00
26.0026.0036.47
7.5016.5024.00
4.0018.0023.50
Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl
1.001.001.001.002.001.001.000.101.00
Components mg/LInorganic Salts
Calcium chloride x 2H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphate anhydrousSodium chloridedi-Sodium hydrogen phosphate
185.44139.52400.00
60.00
8,000.0047.88
OtherCompo-nents
D(+)-Glucose anhydrousHepesPhenol red
1,000.005,958.00
10.00AminoAcids
L-Arginine x HClL-CystineL-GlutamineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine
21.0012.00
292.008.00
26.0026.0036.47
7.5016.5024.00
4.0018.0023.50
Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl
1.001.001.001.002.001.001.000.101.00
Composition
also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa cells. With its many variations it is used in many fields of science today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells.
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Media
Liquid Media with 4.5 g/L Glucose
DMEM with 4.5 g/L Glucose(1)
without L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03500
DMEM with 4.5 g/L Glucose(1)
without L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03600
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03550
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03590
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewith 1.5 g/L NaHCO3 500 ml P04-03596
DMEM with 4.5 g/L Glucose(1)
with stab. Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-04500
DMEM with 4.5 g/L Glucose(1)
with stab. Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-04510
DMEM with 4.5 g/L Glucose(1)
without L-Glutaminewithout Sodium pyruvate without Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01161
DMEM with 4.5 g/L Glucose(1)
without Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01158
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-03591
Media
Page 45Page 44
Special Media with 1.0 g/L Glucose
DMEM with 1.0 g/L Glucose(2)
without L-Glutaminewith Sodium pyruvatewithout Calciumwith 3.7 g/L NaHCO3 500 ml P04-01501
SILAC-DMEM(2)
with 1.0 g/L Glucosewith stab. Glutaminewith Sodium pyruvatewithout L-Argininwithout L-Lysinwith 3.7 g/L NaHCO3 500 ml P04-02501
DMEM with 1.0 g/L Glucose(2)
without L-Glutaminewithout Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-03556
DMEM with 1.0 g/L Glucose(2)
without L-Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01159
DMEM with 1.0 g/L Glucose(2)
with stab. Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-02500S1
DMEM with 1.0 g/L Glucose(2)
with L-Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01516
DMEM with 1.0 g/L Glucose(2)
with L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01555
Dulbecco´s Modified Eagle MediumDulbecco´s Modified Eagle MediumDescriptionIntrinsically developed for the cultivation of murine embryonic cells, DMEM is tailor-made for the cultivation of a broad range of cells, especially if the medium is supplemented with FBS. DMEM is an Eagle medium modification with a four-fold content of amino acids and vitamins. DMEM with 1.0 g/L Glucose is the standard medium, whereas DMEM with 4.5 g/L Glucose is for cells which have a high energy demand.
Liquid Media without Glucose
DMEM without Glucose(1)
without L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01548S1
DMEM without Glucose(1)
without L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01549
DMEM without Glucose(1)
without L-Glutaminewithout Sodium pyruvatewithout Phenol red with 3.7 g/L NaHCO3 500 ml P04-01548
Special Media without Glucose
DMEM without Glucose(2)
with L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01551
Powder Media without Glucose
DMEM without Glucose(1)
without L-Glutaminewithout Sodium pyruvatewithout Phenol red 10 L P03-0010without NaHCO3 50 L P03-0050
Liquid Media with 1.0 g/L Glucose
DMEM with 1.0 g/L Glucose(1)
without L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01500
DMEM with 1.0 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01550
DMEM with 1.0 g/L Glucose(1)
with stab. Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-02500
DMEM with 1.0 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01515
DMEM with 1.0 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewith 25 mM Hepeswith 3.7 g/L NaHCO3 500 ml P04-05551
Powder Media with 1.0 g/L Glucose
DMEM with 1.0 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvate 10 L P03-0510with 3.7 g/L NaHCO3 50 L P03-0550
DMEM with 1.0 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewithout Phenol red 10 L P03-01510with 3.7 g/L NaHCO3 50 L P03-01550
Media
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MediaMedia
Dulbecco’s Modified Eagle Medium
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Media
When 5,957.50 mg/L HEPES is included there is only 5,400.00 mg/L sodium chloride.
CompositionPowder Media with 4.5 g/L Glucose
DMEM with 4.5 g/L Glucose(1)
without L-Glutamine without Sodium pyruvate 10 L P03-6510without NaHCO3 50 L P03-6550
DMEM with 4.5 g/L Glucose(1)
with L-Glutamine without Sodium pyruvate 10 L P03-0710without NaHCO3 50 L P03-0750
DMEM with 4.5 g/L Glucose(1)
with L-Glutamine with Sodium pyruvate 10 L P03-0810without NaHCO3 50 L P03-0850
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewithout Sodium pyruvate with 25 mM Hepes 10 L P03-0910without NaHCO3 50 L P03-0950
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewith Sodium pyruvatewith 25 mM Hepes 10 L P03-1010without NaHCO3 50 L P03-1050
Media
Page 46
Media
Page 47
Components mg/LInorganic Salts
Calcium chloride anhydrous Iron(III) nitrate x 9H2OMagnesium sulfate anhydrousPotassium chlorideSodium chlorideSodium dihydrogen phosphate anhydrous
200.000.10
97.66400.00
6,400.00108.69
OtherCompo-nents
D(+)-Glucose anhydrousHepesPhenol redSodium pyruvate
4,500.005,958.00
15.00110.00
AminoAcids
L-Arginine x HClL-Cystine x 2HClL-GlutamineGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2NaL-Valine
84.0062.58
584.0030.0042.00
104.80104.80146.20
30.0066.0042.0095.2016.00
103.7993.60
Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HCl
4.004.004.007.004.004.000.404.00
Dulbecco´s Modified Eagle Medium
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewith 25 mM Hepeswithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-05540
DMEM with 4.5 g/L Glucose(1)
with L-Glutaminewith 25 mM Hepeswith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-05550
Special Media with 4.5 g/L Glucose
DMEM with 4.5 g/L Glucose(2)
with stab. L-Glutaminewith 25 mM Hepeswithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-04550
DMEM with 4.5 g/L Glucose(2)
with L-Glutaminwithout Sodium pyruvatewith 25 mM Hepeswithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-05545
DMEM with 4.5 g/L Glucose(2)
with stab. Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-03588
DMEM with 4.5 g/L Glucose(2)
without L-Glutaminewith 25 mM Hepeswith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01597
DMEM (10 x)(3) with 4.5 g/L Glucosewithout L-Glutaminewithout Sodium pyruvate with NEAAwithout NaHCO3 500 ml P04-03510
DMEM with 4.5 g/L Glucose(2)
without L-Glutaminewith Sodium pyruvate without L-Argininewith 3.7 g/L NaHCO3 500 ml P04-03598
DMEM with 4.5 g/L Glucose(2)
with L-Glutaminewith 25 mM Hepeswithout Sodium pyruvatewith 2.2 g/L NaHCO3 500 ml P04-04057
DMEM with 4.5 g/L Glucose(2)
with L-Glutaminewithout Sodium pyruvatewithout Sodium chloridewithout NaHCO3 500 ml P04-03560
DMEM with 5.5 g/L Glucose(2)
with L-Glutmaminwithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03551
DMEM with 4.5 g/L Glucose(2)
with stab. Glutaminewith Sodium pyruvatewith 25 mM Hepeswithout Phenol redwith 0.5 g/L NaHCO3 500 ml P04-01163
DMEM with 4.5 g/L Glucose(2)
with L-Glutaminewithout Sodium pyruvatewithout Isoleucinewith 3.7 g/L NaHCO3 500 ml P04-03503
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Media
DMEM/F12Powder Media
DMEM/F12 (1:1)(1)
without L-Glutamine 10 L P03-6010without NaHCO3 50 L P03-6050
DMEM/F12 (1:1)(1)
with L-Glutamine 10 L P03-1110without NaHCO3 50 L P03-1150
DMEM/F12 (1:1)(1)
with L-Glutaminewith 15 mM Hepes 10 L P03-6110without NaHCO3 50 L P03-6150
DMEM/F12 (1:1)(1)
with L-Glutaminewith 25 mM Hepes 10 L P03-1210without NaHCO3 50 L P03-1250
Media
Page 48
Media
Page 49
Liquid Media
DMEM/F12 (1:1)(1)
without L-Glutaminewith 1.2 g/L NaHCO3 500 ml P04-41450
DMEM/F12 (1:1)(1)
with L-Glutaminewith 1.2 g/L NaHCO3 500 ml P04-41500
DMEM/F12 (1:1)(1)
with stab. Glutaminewith 1.2 g/L NaHCO3 500 ml P04-41150
DMEM/F12 (1:1)(1)
without L-Glutaminewith 15 mM Hepeswith 1.2 g/L NaHCO3 500 ml P04-41550
DMEM/F12 (1:1)(1)
with L-Glutaminewith 15 mM Hepeswith 1.2 g/L NaHCO3 500 ml P04-41250
Special Media
DMEM/F12 (1:1)(2)
without L-Glutaminewithout Glucosewith1.2 g/L NaHCO3 500 ml P04-41151
DMEM/F12 (1:1)(2)
with L-Glutaminewith 25 mM Hepeswith 1.2 g/L NaHCO3 500 ml P04-41252
DMEM/F12 (1:1)(2)
with L-Glutaminewithout Phenol red with 1.2 g/L NaHCO3 500 ml P04-41650
DMEM/F12 (1:1)(2)
with stab. Glutaminewith 15 mM Hepeswithout Calcium chloridewith 1.2 g/L NaHCO3 500 ml P04-41251
DMEM/F12DescriptionThis medium supports the growth of almost all cell lines. For example, it is used for pancreas cells, Sertoli cells or to culture cells, which are used for human protein production. It combines the advantages of both media DMEM (high concentration of amino acids and vitamins) and Ham‘s F12 (higher concentration of zinc sulphate, putrescine and linoleic acid).
Composition
Components mg/LInorganic Salts
Calcium chloride x 2H2OIron(III)-nitrate x 9H2OIron(II)-sulfate x 7H2OPotassium chlorideCopper(II)-sulfate x 5H2OMagnesium chlorideMagnesium sulfateSodium chlorideSodium dihydrogen phosphatedi-Sodium hydrogen phosphateZinc sulfate x 7H2O
154.450.050.42
311.830.00128.5748.85
6,999.5054.3570.98
0.43OtherCompo-nents
D(+)-Glucose anhydrousHypoxanthine Linoleic acidDL-68-Lipoic acidSodium pyruvatePhenol redPutrescin x 2HClThymidine
3,151.02.040.04
0.103110.00
8.100.081
0.36AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cystine x 2HClL-Cysteine x HCl x H2OL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2Na x 2H2OL-Valine
4.45147.35
7.506.65
31.2917.56
365.007.35
18.7531.4854.3758.9691.3717.2435.4817.2726.2553.55
9.0255.8153.00
Vitamins D-(+)-BiotineD-Calcium pantothenateCholin chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamine B12
0.0042.128.982.66
12.512.022.030.222.170.68
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Media
Grace´s Insect MediumDescriptionThe Grace‘s Insect Medium was originally developed to culture insect cells including SF9 and SF21 cells. Moreover it supports a broad range of lepidopteran cells.
Composition
Special Media
Grace‘s Insect Medium(2)
without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-81500
Grace’s Insect Medium(2)
with L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-82500
Powder Media
Grace’s Insect Medium(1)
without L-Glutamine 10 L P03-9010without NaHCO3 50 L P03-9050
Grace’s Insect Medium(1)
with L-Glutamine 10 L P03-9110without NaHCO3 50 L P03-9150
Media
Page 50
Media
Page 51
Components mg/LInorganic Salts
Calcium chloride x 2H2OPotassium chlorideMagnesium chloride x 6H2OMagnesium sulfate drieddi-Sodium hydrogen phosphate
1,324.62,240.002,278.861,939.81
876.92
OtherCompo-nents
DL-Malic acidSuccinic acidFructoseFumaric acidD(+)-Glucose anhydrousα-Ketoglutaric acid sodium saltD-Sucrose
670.0060.00
400.0055.00
700.00425.66
26,680.00AminoAcids
ß-AlanineL-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
200.00200.00700.00350.00350.00
19.18600.00600.00650.00
2,500.0050.0075.00
625.0050.00
150.00350.00550.00175.00100.00
50.00100.00
Vitamins p-Aminobenzoic acidD(+)-BiotinD-Ca-PantothenateCholine chlorideFolic acidmyo-InositolNiacinPyridoxine x HClRiboflavinThiamine x HCl
0.020.010.020.200.020.020.020.020.020.02
Glasgow MEM (BHK 21)DescriptionThe GMEM was developed as a modification of BME to culture primary baby hamster kidney cells. This version has twice the concentration of vitamins and amino acids.
Composition
Liquid Media
Glasgow-MEM (BHK 21)(1)
without L-Glutaminewithout Tryptose phosphate with 2.75 g/L NaHCO3 500 ml P04-97500
Glasgow-MEM (BHK 21)(1) with L-Glutaminewith Tryptose phosphate with 2.75 g/L NaHCO3 500 ml P04-96500
Special Media
Glasgow-MEM (BHK 21)(2) without L-Glutaminewith Tryptose phosphate with 2.75 g/L NaHCO3 500 ml P04-98500
Powder Media
Glasgow-MEM (BHK 21)(1) without L-Glutamine without Tryptose phosphate 10 L P03-3110without NaHCO3 50 L P03-3150
Glasgow-MEM (BHK 21) (1) with L-Glutamine with Tryptose phosphate 10 L P03-6910without NaHCO3 50 L P03-6950
Glasgow-MEM (BHK 21)(1) with L-Glutamine without Tryptose phosphate 10 L P03-6810without NaHCO3 50 L P03-6850
Components w/oTryptosePhosph-ate mg/L
withTrytose
Phosph-ate mg/L
Inor-ganic Salts
Calcium chloride x 2H2OIron(III) nitrate x 9H2OMagnesium sulfate driedPotassium chlorideSodium chloridedi-Sodium hydrogen phosphateSodium dihydrogen phosphate x H2O
264.920.10
139.57400.00
6,400.000.00
124.00
238.430.09
125.64360.00
6,260.00250.00
111.60
OtherCom-po-nents
D(+)-Glucose anhydrousPhenol redPepton from CaseinPepton from meatYeast extract
4,500.0015.00
0.000.000.00
4,250.0013.50
1,000.00500.00500.00
Ami-noAcids
L-Arginine x HClL-CystineL-GlutamineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine
42.0024.00
292.0021.0052.4052.4073.1015.0033.0047.60
8.0036.2046.80
37.8021.60
262.8018.9047.1647.1665.7913.5029.7042.84
7.2032.5242.12
Vita-mins
D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl
2.002.002.003.602.002.000.202.00
1.801.801.803.241.801.800.181.80
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MediaMedia
DescriptionIn the past Ham‘s F12 was the first choice for a serum-free cultivation of CHO-cells and is now substituted through better serum-free systems like our Panserin C6000, which is protein-free in addition. However, Ham‘s F12 is an appropriate medium for mammalian cells when it is supplemented with FBS. It contains a high concentration of vitamins, amino acids and trace elements. The content of zinc sulphate is increased and it contains putrescine and linoleic acid.
Ham´s F12 MediumComposition
Liquid Media
Ham’s F12 Medium(1)
without L-Glutaminewith 1.176 g/L NaHCO3 500 ml P04-14550
Ham’s F12 Medium(1)
with L-Glutaminewith 1.176 g/L NaHCO3 500 ml P04-14500
Ham’s F12 Medium(1)
with stab. Glutaminewith 1.176 g/L NaHCO3 500 ml P04-15500
Special Media
Ham’s F12 Medium(2)
with L-Glutaminewithout Phenol redwith 25 mM Hepeswith 1.176 g/L NaHCO3 500 ml P04-14501
Ham’s F12 Medium(2)
without L-Glutaminewithout Phenol redwith 1.176 g/L NaHCO3 500 ml P04-14559
Ham´s F12K Medium(2)
with L-Glutaminewith 2.5 g/L NaHCO3 500 ml P04-15600
Powder Media
Ham’s F12 Medium(1)
with L-Glutamine 10 L P03-4110without NaHCO3 50 L P03-4150
When 5,958.00 mg/L HEPES is included there is only 7,099.00 mg/L sodium chloride.
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MediaMedia
Page 52
Media
Page 53
Components mg/LInorganic Salts
Calcium chloride anhydrous Copper(II) sulfate x 5H2OIron(II) sulfate x 7H2OMagnesium chloride x 6H2OPotassium chlorideSodium chloridedi-Sodium hydrogen phosphate anhydrousZinc sulfate x 7H2O
33.300.0030.834
122.00223.657599.9142.04
0.86OtherCompo-nents
D(+)-Glucose anhydrousHepesHypoxanthineLinoleic acidDL-Lipoic acidPhenol redPutrescine x 2HClSodium pyruvateThymidine
1801.605958.00
4.080.084
0.211.200.16
110.000.73
AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cysteine x HCl x H2OL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
8.91210.70
15.0113.3135.12
146.2014.71
7.5120.96
3.9413.1236.54
4.484.96
34.5310.5111.91
2.045.44
11.71Vitamins D(+)-Biotin
D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12
0.0070.24
13.961.32
18.000.0370.0620.038
0.341.36
Ham´s F10 MediumDescriptionHam‘s F10 is an alternative to Ham‘s F12 and it was used primarily to culture CHO-cells. Today, Ham‘s F10 can be used with or without FBS for many different cell cultures. It is used for example for primary cells of rat and chicken, but also for human diploid cells.
Composition
When 5,958.00 mg/L HEPES is included there is only 6,900.00 mg/L sodium chloride.
Liquid Media
Ham’s F10 Medium(1)
with L-Glutaminewith 1.2 g/L NaHCO3 500 ml P04-12500
Special Media
Ham‘s F10 Medium(2)
without L-Glutaminewith 1.2 g/L NaHCO3 500 ml P04-12050
Ham’s F10 Medium(2)
with stab. Glutaminewith 1.2 g/L NaHCO3 500 ml P04-13500
Ham’s F10 Medium(2)
with L-Glutaminewith 25 mM Hepeswith 1.2 g/L NaHCO3 500 ml P04-13050
Ham’s F10 Medium(2)
without L-Glutaminewithout Phenol redwith 1.2 g/L NaHCO3 500 ml P04-12049
Powder Media
Ham‘s F-10 Medium(1) without L-Glutamine 10 L P03-5010 without NaHCO3 50 L P03-5050
Ham’s F-10 Medium(1) with L-Glutamine 10 L P03-3910without NaHCO3 50 L P03-3950
Ham’s F-10 Medium(1)
with L-Glutamine with 25 mM Hepes 10 L P03-4010without NaHCO3 50 L P03-4050
Components mg/LInorganic Salts
Calcium chloride x 2H2OCopper(II) sulfate x 5H2OIron(II) sulfate x 7H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium chloridedi-Sodium hydrogen phosphateZinc sulfate x 7H2O
44.090.0030.834
106.06285.00
83.00
7400.00153.70
0.029OtherCompo-nents
D(+)-Glucose anhydrousHypoxanthineDL-α-Lipoic acidHepesPhenol redSodium pyruvate2´- Deoxythymidine
1100.004.080.21
5958.001.20
110.000.73
AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cysteine x HCl x H2OL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
8.91211.00
15.0013.3035.12
146.2014,70
7.5121.00
2.6013.1029.30
4.484.96
11.5010.50
3.570.601.813.50
Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12
0.0240.7150.698
1.320.5410.615
0.210.376
1.011.36
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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MediaMedia
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Media
Iscove´s Modified Dulbecco´s MediumDescriptionThe IMDM is a modified DMEM, with a higher content of vitamins, selenium and amino acids. As it is supplemented with albumin, transferrin and soy lipids it can be excellently applied for culturing lymphocytes, marrow cells or hybridoma cells. Note: for hybridomas there is a better and highly efficient protein-free medium available: our Panserin H4000.
Composition
When 5,958.00 mg/L HEPES is included there is only 4,505.00 mg/L sodium chloride.
Liquid Media
IMDM(1)
without L-Glutaminewith 3.024 g/L NaHCO3 500 ml P04-20250
IMDM(1)
with L-Glutaminewith 3.024 NaHCO3 500 ml P04-20350
IMDM(1)
without L-Glutaminewith 25 mM Hepeswith 3.024 g/L NaHCO3 500 ml P04-20050
IMDM(1) with L-Glutaminewith 25 mM Hepeswith 3.024 g/L NaHCO3 500 ml P04-20150
Media
Page 54 Page 55
Components mg/LInorganic Salts
Calcium chloride anhydrousPotassium chloridePotassium nitrateMagnesium sulfate anhydrousSodium chlorideSodium dihydrogen phosphate x H2OSodium selenite x 5H2O
165.00330.00
0.07697.66
5,005.00125.00
0.01OtherCompo-nents
D(+)-Glucose anhydrousHepesSodium pyruvatePhenol red
4500.005958.00
110.0015.00
AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cystine x 2HClL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2Na x 2H2OL-Valine
25.0084.0028.4030.0091.24
584.0075.0030.0042.00
105.00105.00146.00
30.0066.0040.0042.0095.0016.00104.294.00
Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12
0.01304.004.004.007.204.004.000.404.00
0.013
Iscove´s Modified Dulbecco´s MediumSpecial Media
IMDM(2) without L-Glutaminewith 1.0 g/L Glucosewith 3.024 g/L NaHCO3 500 ml P04-20259
IMDM(2)
with stab. Glutaminewith 25 mM Hepeswithout Phenol red315 mOsmwith 3.024 g/L NaHCO3 500 ml P04-20451S1
IMDM(2) with L-Glutaminewith 25 mM Hepes320 mOsmwith 3.024 g/L NaHCO3 500 ml P04-20150S2
IMDM(2)
with L-Glutaminewith 1.5 g/L NaHCO3 500 ml P04-20351
IMDM(2) with stab. Glutaminewith 25 mM Hepeswith 3.024 g/L NaHCO3 500 ml P04-20450
IMDM(2)
with stab. Glutaminewith 25 mM Hepeswithout Phenol redwith 3.024 g/L NaHCO3 500 ml P04-20451
Powder Media
IMDM(1)
without L-Glutamine 10 L P03-5210without NaHCO3 50 L P03-5250
IMDM(1) with L-Glutamine 10 L P03-1310without NaHCO3 50 L P03-1350
IMDM(1) with L-Glutaminewith 25 mM Hepes 10 L P03-1410without NaHCO3 50 L P03-14503 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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MediaMedia
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Media
IPL-41 Insect MediumDescriptionIPL-41 is primarly used for the growth and maintenance of lepidopteran cells and for the propagation of viruses in these cells lines. The medium is also used for long time culture of baculo-virus infected spodotera cells.
Composition
Special Media
IPL-41 Insect Medium(2)
with L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-85600
Powder Media
IPL-41 Insect Medium(1)
without L-Glutamine 10 L P03-9210without NaHCO3 50 L P03-9250
Media
Page 56 Page 57
Components mg/LInorganic Salts
Ammonium heptamolybdate x 4H2OCalcium chloride x 2H2OCobalt(II) chloride x 6H2OCopper chloride x 2H2OIron(II) sulfate x 7H2OMagnesium sulfate driedManganese chloride x 4H2OPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2OZinc chloride
0.04
662.310.050.200.55
1311.400.02
1200.002850,001160.00
0.04OtherCompo-nents
Fumaric acidD(+)-Glucose anhydrousα-Ketoglutaric acid sodium saltDL-Malic acidD-Maltose x H2OSuccinic acidSucrose
4.402500.00
34.0553.60
1052.584.80
1650.00AminoAcids
β-AlanineL-Arginine x HClL-Aspartic acidL-Asparagine x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
300.00800.00
1300.001477.14
100.001000.001500.00
200.00200.00800.00750.00250.00700.00
1000.001000.00
500.00200.00200.00100.00250.02500.00
Vitamins p-Aminobenzoic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinic acidNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamine B12
0.320.16
0.00820.00
0.080.400.160.160.400.080.080.24
Joklik-MEMDescriptionJoklik´s MEM is a modification of MEM for suspension cultures. Due to the absence of calcium chloride in this formulation the attachment of cells is reduced.
Composition
Liquid Media
Joklik – MEM(1)
Hepes Mediumwith L-Glutaminewith 3.6 g/L Hepes 500 ml P04-21300
Special Media
Joklik - MEM(2) modified for spinner culturewith EBSS (modified)without L-Glutaminewithout Antibioticawithout Calciumchloridewith 2.0 g/L NaHCO3 500 ml P04-21200
Powder Media
Joklik - MEM(1)
modified for spinner culturewith EBSS (modified)without L-Glutaminewithout Antibioticawithout Calciumchloride 10 L P03-02010Pwithout NaHCO3 50 L P03-02050P
Components mg/LInorganic Salts
Magnesium chloride x 6H2OPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O
200.00400.00
6,500.001,327.00
OtherCompo-nents
D(+)-Glucose anhydrousPhenol red
2,000.0010.00
AminoAcids
L-Arginine x HClL-CystineL-GlutamineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x H2OL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine
126.0024.00
294.0031.0052.0052.0065.0015.0032.0048.0010.0032.6046.00
Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl
1.001.001.002.001.001.000.101.00
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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Media
Leibovitz´s L-15 MediumDescriptionL-15 contains no sodium hydrogen carbonate and no bicarbonate, because it is buffered already by a high concentration of amino acids. The L-15 medium supports the growth of established cells like Hep-2, but also human nerve cells and primary tissue explants. With 10 % tryptose phosphate broth it is also ideally suited for the cultivation of insect cell lines.
Composition
Liquid Media
Leibovitz’s L-15 Medium(1)
without L-Glutaminewithout NaHCO3 500 ml P04-27055
Leibovitz’s L-15 Medium(1) with L-Glutaminewithout NaHCO3 500 ml P04-27500
Special Media
Leibovitz’s L-15 Medium(2) with stab. Glutaminewithout NaHCO3 500 ml P04-27050
Leibovitz’s L-15 Medium(2) without L-Glutaminewithout Phenol redwithout NaHCO3 500 ml P04-27054
Powder Media
Leibovitz’s L-15 Medium(1)
with L-Glutamine 10 L P03-1510without NaHCO3 50 L P03-1550
Leibovitz’s L-15 Medium(1)
with L-Glutaminewith 25 mM Hepes 10 L P03-1610without NaHCO3 50 L P03-1650
Media
Page 58 Page 59
Components mg/LInorganic Salts
Calcium chloride x 2H2OMagnesium chloride x 6H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium chloridedi-Sodium hydrogen phosphate
185.44200.00139.53400.00
60.00
8,000.00190.00
OtherCompo-nents
D(+)-Galactose anhydrousHepesPhenol redSodium pyruvate
900.005,958.00
10.00550.00
AminoAcids
L-AlanineL-Arginine BaseL-Asparagine x H2OL-CysteineL-GlutamineGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
225.00500.00250.00120.00300.00200.00250.00250.00125.00
93.7575.00
125.00200.00300.00
20.00300.00100.00
Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavin-5’-phosphate sodium salt x 2H2OThiamine monophosphate chloride x2 H2O
1.001.001.002.001.001.00
0.1075
1.00
When 5,958.00 mg/L HEPES is included there is only 7,500.00 mg/L sodium chloride.
Mc Coy´s 5A MediumDescriptionMc Coy‘s 5A Medium is a complete medium with all amino acids and vitamins. It is used for growing primary cultures. This group contains marrow cells, gingival cells, adrenal cells, spleen cells, lung cells, rat embryos and other cell types.
Composition
When 5,958.00 mg/L HEPES is included there is only 5,960.00 mg/L sodium chloride.
Powder Media
McCoy’s 5A Medium (modified)(1) with L-Glutamine 10 L P03-1710without NaHCO3 50 L P03-1750
McCoy’s 5A Medium (modified)(1)
with L-Glutamine with 25 mM Hepes 10 L P03-1810without NaHCO3 50 L P03-1850
Liquid Media
Mc Coy’s 5A Medium (modified)(1)
with L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-05500
Mc Coy’s 5A Medium (modified)(1)
with stab. Glutaminewith 2.2 g/L NaHCO3 500 ml P04-06500
Special Media
Mc Coy’s 5A Medium (modified)(2)
with L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-05050
Mc Coy’s 5A Medium(2) without L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-05610
Components mg/LInorganic Salts
Calcium chloride x 2H2OMagnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O
132.46139.53400.00
6,460.00580.00
OtherCompo-nents
D(+)-Glucose anhydrousGlutathione (red.)HepesBacto - PeptonePhenol red
3,000.000.50
5,958.00600.00
10.00AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CysteineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
13.3642.1045.0019.9724.24
219.2022.10
7.5020.7619.7039.3639.3636.5014.9016.5017.3026.3017.90
3.1018.1017.60
Vitamins p-Aminobenzoic acidAscorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamideNicotinic acidPyridoxal x HClPyridoxine x HClRiboflavinThiamine x HClVitamin B12
1.000.500.200.205.00
10.0036.00
0.500.500.500.500.200.202.00
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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Page 60 Page 61
DescriptionMCDB 131 is a medium for the cultivation of human micro-vascular endothelial cells under reduced serum content. For this purpose it has be supplemented with dialyzed serum, EGF and hydrocortisone.
Liquid Media
MCDB 131(1) without L-Glutaminewith 1.176 g/L NaHCO3 500 ml P04-80057
Special Media
MCDB 131(2) with L-Glutaminewith 1.176 g/L NaHCO3 500 ml P04-80053
MCDB 131(2)
without Glutamine with 25 mM Hepeswith 1.176 g/L NaHCO3 500 ml P04-80054
Components mg/LInorganic Salts
Ammonium MetavandateCalcium Chloride x 2H2OCopper(II) Sulfate x 5H2OIron (III) sulfate x 7H2OMagnesiumsulfate driedManganese Sulfate x H2OAmmonium Molybdate x 4H2ONickel Chloride x 6H2OPotassium ChlorideSodium ChlorideSodium Metasilicate x 5H2ODi-Sodium hydrogen phosphateSodium Selenite anhydrousZinc Sulfate x 7H2O
0.0006235.050.0012
0.2831565.20
0.00020.00370.0007298.00
6,430.002.09
71.000.00390.0003
OtherCompo-nents
AdenineD-GlucoseDL-α-Lipoic acid Phenol RedPutrescine x 2HClSodium pyruvate2‘Deoxythymidine
0.1351,000.00
0.002110.000.002
110.000.024
AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic AcidL-Cysteine x HCl x H2OL-Glutamic AcidL-GlutamineGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
2.7063.2015.0013.3035.00
4.001,461.00
2.3042.0066.00
131.00182.00
15.0033.0011.5032.0012.00
4.1018.10
117.10Vitamins D-Biotin
Choline ChlorideFolic Acidmyo-Inositol NiacinamideD-Calcium-pantothenate Pyridoxine x HClRiboflavinThiamine x HClVitamin B12
0.007313.96
0.607.206.10
12.002.10
0.00383.40
0.0136
MCDB 131 Medium Medium 199 with Earle´s SaltsDescriptionThe M199 was originally developed to assay the nutrient demand of embryonic chicken fibroblasts. But it works very well with cells from many different animal species. For example, it is used for vaccine production in virology. For long term cultures serum should be added.
Composition
When 5,958.00 mg/L HEPES is included there is only 6,300.00 mg/L sodium chloride.
Liquid Media
M199 with EBSS(1)
without L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-07500
Special Media
M199 with EBSS(2)
with L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-07050
M199 with EBSS(2)
with stab. Glutaminewith 2.2 g/L NaHCO3 500 ml P04-07250
M199 with EBSS(2)
with L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-07150
Powder Media
M199 with EBSS(1)
with L-Glutamine 10 L P03-1910without NaHCO3 50 L P03-1950
Components mg/LInorganic Salts
Calcium chloride x 2H2OIron(III) nitrate x 9H2OMagnesium sulfate driedPotassium chlorideSodium acetate x 3H2OSodium chlorideSodium dihydrogen phosphate
264.920.72
139.52400.00
82.956,800.00
140.00OtherCompo-nents
Adenine sulfateAMPATPCholesterol2’-DeoxyriboseD(+)-Glucose anhydrousGlutathione (red,)Guanine x HClHepesHypoxanthinePhenol redD-RiboseThymineTween 80UracilXanthine
10.000.201.000.200.50
1,000.000.050.30
5,958.000.30
10.000.500.304.900.300.30
AminoAcids
L-AlanineL-Arginine x HClL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
25.0070.0030.00
0.1020.00
100.0067.0050.0021.8810.0020.0060.0070.0015.0025.0040.0025.0030.0010.0040.0025.00
Vitamins p-Aminobenzoic acidAscorbic acidD(+)-BiotinCalciferolD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolMenadioneNicotinic acidNicotinamidePyridoxal x HClPyridoxol x HClRiboflavinDL-α-Tocopherol phosphate- Na2Thiamine x HClVitamin A acetate
0.050.050.010.100.010.500.010.050.01
0.0250.0250.0250.025
0.010.01
0.010.14
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
Composition
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Medium 199 with Hank´s SaltsSpecial Media
M199 with HBSS(2)
without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-07753
M199 with HBSS(2)
with L-Glutaminewith 25 mM Hepeswith 0.35 g/L NaHCO3 500 ml P04-07450
M199 with HBSS (10X)(2)
without L-Glutaminewithout NaHCO3 500 ml P04-07600
Powder Media
M199 with HBSS(1)
with L-Glutamine 10 L P03-2110 without NaHCO3 50 L P03-2150
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MediaMedia
MEM with Earle´s SaltsDescriptionMEM is an advancement of the BME and the base medium of many further modifications. Because BME did not fulfil all requirements for some mammalian and HeLa cells, a better variation had to be developed. Today, MEM is one of the most used synthetic media and shows its versatility by supplementing with amino acids including Hank´s or Earle´s salts. Even the addition of only small amounts of FBS results in a positive effect on cell growth.
Composition
When 5,958.00 mg/L HEPES is included there is only 6,300.00 mg/L sodium chloride.
Liquid Media without Glutamine
MEM Eagle with EBSS(1)
without L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-08050
MEM Eagle with EBSS(1) without L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-08150
Special Media without Glutamine
MEM Eagle with EBSS(2)
without L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-00507
MEM Eagle with EBSS(2)
without L-Glutaminewith NEAAwith 2.2 g/L NaHCO3 500 ml P04-08509
MEM Eagle with EBSS(2)
without L-Glutaminewithout NaHCO3 500 ml P04-09050
Powder Media without Glutamine
MEM Eagle with EBSS(1)
without L-Glutamine 10 L P03-7410without NaHCO3 50 L P03-7450
Page 62
Media
CompositionComponents mg/L
Inorganic Salts
Calcium chloride x2H2OIron(III) nitrate x 9H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium acetate x 3H2OSodium chloridedi-Sodium hydrogen phosphate
185.450.72
139.68400.00
60.00
83.008,000.00
47.68OtherCompo-nents
Adenine sulfateAMPATPCholesterol2’-DeoxyriboseD(+)-Glucose anhydrousGlutathione (red.)Guanine x HClHepesHypoxanthinePhenol redD-RiboseThymineTween 80UracilXanthine
10.000.201.000.200.50
1,000.000.050.30
5,958.000.30
10.000.500.304.900.300.30
AminoAcids
L-AlanineL-Arginine x HClL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
25.0070.0030.00
0.1020.00
100.0067.0050.0021.8810.0020.0060.0070.0015.0025.0040.0025.0030.0010.0040.0025.00
Vitamins p-Aminobenzoic acidAscorbic acidD(+)-BiotinCalciferolD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolMenadioneNicotinic acidNicotinamidePyridoxal x HClPyridoxol x HClRiboflavinDL-α-Tocopherol phosphate- Na2Thiamine x HClVitamin A acetate
0.050.050.010.100.010.500.010.050.01
0.0250.0250.0250.025
0.010.01
0.010.14
Components mg/LInorganic Salts
Calcium chloride x2H2OMagnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O
264.92139.53400.00
6800.00140.00
OtherCompo-nents
D(+)-Glucose anhydrousHepesPhenol red
1000.005958.00
10.00AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
8.90126.00
13.2013.3024.00
292.0014.70
7.5042.0052.0052.0072.5015.0032.0011.5010.5048.0010.0036.0046.00
Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl
1.001.001.002.001.001.000.101.00
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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Media
Page 64
MEM with Earle´s SaltsLiquid Media with L-Glutamine
MEM Eagle with EBSS(1)
with L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-08500
MEM Eagle with EBSS(1)
with L-Glutaminewith 1.5 g/L NaHCO3 500 ml P04-00509
Special Media with L-Glutamine
MEM Eagle with EBSS(2)
with L-Glutaminewithout Phenol redwith 1.5 g/L NaHCO3 500 ml P04-00508
MEM Eagle with EBSS(2)
with L-Glutaminewith 20 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-08549
MEM Eagle with EBSS(2)
with L-Glutaminewith NEAAwith 2.2 g/L NaHCO3 500 ml P04-08510
MEM Eagle with EBSS(2)
with 2 mM Glutaminewith 1 mM Pyruvatewith NEAAwith 1.5 g/L NaHCO3 500 ml P04-08056
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MediaMedia
MEM with Hank´s SaltsComposition
When 5,958.00 mg/L HEPES is included there is only 7,500.00 mg/L sodium chloride.
Special Media
MEM Eagle with HBSS(2)
without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-10050
MEM Eagle with HBSS(2)
with L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-10500
MEM Eagle with HBSS(2)
with L-Glutaminewith 0.60 g/L NaHCO3 500 ml P04-10599
Powder Media
MEM Eagle with HBSS(1)
with L-Glutamine 10 L P03-3310without NaHCO3 50 L P03-3350
Powder Media with L-Glutamine
MEM Eagle with EBSS(1)
with L-Glutaminewith NEAA 10 L P03-2910without NaHCO3 50L P03-2950
MEM Eagle with EBSS(1)
with L-Glutaminewith NEAA with 25 mM Hepes 10 L P03-3010with NaHCO3 50 L P03-3050
MEM Eagle with EBSS(1)
with L-Glutamine 10 L P03-2710without NaHCO3 50 L P03-2750
MEM Eagle with EBSS(1)
with L-Glutaminewith 25 mM Hepes 10 L P03-2810without NaHCO3 50 L P03-2850
Liquid Media with stab. Glutamine
MEM Eagle with EBSS(1)
with stab. Glutaminewith 2.2 g/L NaHCO3 500 ml P04-09500
MEM Eagle with EBSS(1)
with stab. Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-08250
Components mg/LInorganic Salts
Calcium chloride x 2H2OPotassium chloridePotassium dihydrogen phosphate anhydrousMagnesium sulfate driedSodium chloridedi-Sodium hydrogen phosphate
185.44400.00
60.00
139.538,000.00
47.88OtherCompo-nents
D(+)-Glucose anhydrousHepesPhenol red
1,000.005,958.00
10.00AminoAcids
L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
8.90126.00
13.2013.3024.00
292.0014.70
7.5042.0052.0052.0072.5015.0032.0011.5010.5048.0010.0036.0046.00
Vitamins D-Calcium pantothenateCholin chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl
1.001.001.002.001.001.000.101.00
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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Media
Page 66
RPMI 1640DescriptionThe medium was developed for culture of normal and neoplastic leukocytes, but also marrow cells and hybridoma cells. Meanwhile there are better, serum-free media for hybridoma cells such our Panserin H4000. Just by supplementing RPMI 1640 with varying amounts of FBS, a very good medium for many different cell lines can be obtained.
Composition
When 5,958.00 mg/L HEPES is included there is only 5,000.00 mg/L sodium chloride.
Liquid Media without Glutamine
RPMI 1640(1)
without L-Glutaminewith 2.0 g/L NaHCO3 500 ml P04-17500
RPMI 1640(1) without L-Glutaminewithout Phenol redwith 2.0 g/L NaHCO3 500 ml P04-16516
RPMI 1640(1)
without L-Glutaminewith 25 mM Hepeswith 2.0 g/L NaHCO3 500 ml P04-18000
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MediaMedia
RPMI 1640Special Media without Glutamine
RPMI 1640(2) without L-Glutamine without Calciumwith 2.0 g/L NaHCO3 500 ml P04-16151
RPMI 1640(2) without L-Glutaminewithout L-Tryptophanwith 2.0 g/L NaHCO3 500 ml P04-17599
RPMI 1640(2)
without L-Glutaminewithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-17550
RPMI 1640(2)
without L-Glutaminewith 15 mM Hepeswithout Phosphatewith 2.0 g/L NaHCO3 500 ml P04-21049
RPMI 1640 (10X)(2)
without L-Glutaminewithout NaHCO3 500 ml P04-17510
RPMI 1640(2)
without L-Glutaminewith 25 mM Hepeswithout NaHCO3 500 ml P04-17850
RPMI 1640(2) without L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-22500
RPMI 1640(2)
without L-Glutaminewith 20 mM Hepeswith 0.85 g/L NaHCO3 500 ml P04-19500
Powder Media without Glutamine
RPMI 1640(1) without L-Glutamine 10 L P03-7210without NaHCO3 50 L P03-7250
RPMI 1640(1)
without L-Glutamine without Phenol red 10 L P03-7710without NaHCO3 50 L P03-7750
RPMI 1640(1)
without L-Glutaminewith 25 mM Hepes 10 L P03-4410without NaHCO3 50 L P03-4450
Components mg/LInorganic Salts
Calcium nitrate x 4H2OPotassium chlorideMagnesium sulfate anhydrousSodiumchloridedi-Sodium hydrogen phosphate
100.00400.00
48.836000.00
800.49OtherCompo-nents
D(+)-Glucose anhydrousGlutathion (red.)HepesPhenol red
2000.001.00
5958.005.00
AminoAcids
L-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cystine x 2HClL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2NaL-Valine
241.8650.0020.0065.19
300.0020.0010.0020.2720.0050.0050.0040.0015.0015.0020.0030.0020.00
5.0028.8320.00
Vitamins p-Aminobenzoic acidD-(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12
1.000.200.253.001.00
35.001.001.000.201.00
0.005
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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Media
Page 68
RPMI 1640
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MediaMedia
Schneider´s Drosophila MediumDescriptionOriginally developed for the culture of Drosophila cells, this medium is also suitable for the culture of other dipteran cell lines.
Composition
Liquid Media
Schneider’s Drosophila Medium(1)
without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-90500
Schneider’s Drosophila Medium(1)
with L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-91500
Powder Media
Schneider’s Drosophila Medium(1)
without L-Glutamine 10 L P03-9310without NaHCO3 50 L P03-9350
Components mg/LInorganic Salts
Potassium chloridePotassium dihydrogen phosphateMagnesium sulfate driedSodium chloridedi-Sodium hydrogen phosphate
1600.00450.00
2585.712100.00
700.00OtherCompo-nents
DL-Malic acidSuccinic acidFumaric acidD(+)-Glucose anhydrousYeast extractα-Ketoglutaric acid sodium saltD(+)-Trehalose x 2H2O
600.0060.0060.00
2000.002000.00
402.662210.00
AminoAcids
β-AlanineL-Arginine BaseL-Asparatic acidL-Cysteine free baseL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
500.00600.00400.00
60.0016.60
1800.00800.00250.00400.00150.00150.00
1650.00150.00
1700.00250.00350.00100.00500.00300.00
Liquid Media with L-Glutamine
RPMI 1640(1)
with L-Glutaminewith 2.0 g/L NaHCO3 500 ml P04-16500
RPMI 1640(1) with L-Glutaminewithout Phenol redwith 2.0 g/L NaHCO3 500 ml P04-16515
RPMI 1640(1)
with 2 mM L-Glutaminewith 1 mM Sodium pyruvatewith 4.5 g/L Glucosewith 10 mM Hepeswith 1.5 g/L NaHCO3 500 ml P04-18047
RPMI 1640(1) with L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-22100
Special Media with L-Glutamine
RPMI 1640(2) with L-Glutaminewithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-17545
RPMI 1640(2) with L-Glutaminewithout L-Argininewith 2.0 g/L NaHCO3 500 ml P04-16598
RPMI 1640(2)
with L-Glutaminewithout L-Tryptophanwith 2.0 g/L NaHCO3 500 ml P04-17598
RPMI 1640(2) with L-Glutaminewith 20 mM Hepeswith 0.85 g/L NaHCO3 500 ml P04-19550
Liquid Media with stab. Glutamine
RPMI 1640(1)
with stab. Glutaminewith 2.0 g/L NaHCO3 500 ml P04-18500
RPMI 1640(1) with stab. Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-18050
Special Media with stab. Glutamine
RPMI 1640(2) with stab. L-Glutaminewithout Phenol redwith 2.0 g/L NaHCO3 500 ml P04-16520
RPMI 1640(2) with stab. Glutaminewithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-17546
RPMI 1640(2) with stab. Glutaminewithout Phenol redwithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-16530
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
Powder Media with L-Glutamine
RPMI 1640(1) with L-Glutamine 10 L P03-4310without NaHCO3 50 L P03-4350
RPMI 1640(1)
with L-Glutaminewith 25 mM Hepes 10 L P03-7310without NaHCO3 50 L P03-7350
RPMI 1640(1)
with L-Glutaminewithout Phenol red 10 L P03-7610without NaHCO3 50 L P03-7650
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Media
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MediaMedia
TC 100 Insect MediumDescriptionThe TC 100 Insect Medium is an absolutely serum-free formula (Oxford formulation) for the growth of insect cells, especially for SF9 cells and the breeding of viruses. If you would like to work with a modern protein-free insect medium, our Spodopan is the ideal choice.
Composition
Liquid Media
TC 100 Insect Medium(1)
with L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-92500
Special Media
TC 100 Insect Medium(2)
without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-93500
Powder Media
TC 100 Insect Medium(1)
with L-Glutamine 10 L P03-9610without NaHCO3 50 L P03-9650
Components mg/LInorganic Salts
Calcium chloride x 2H2OPotassium chlorideMagnesium chloride x 6H2OMagnesium sulfate driedSodium dihydrogen phosphate x H2O
1298.132900.002282.591781.00
970.00
OtherCompo-nents
D(+)-Glucose anhydrousBacto - Tryptose
1000.002600.00
AminoAcids
L-AlanineL-Arginine BaseL-Aspartic acidL-Asparagine x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
225.00550.00350.00391.97
20.00600.00600.00650.00
3400.0050.0075.00
630.0050.00
150.00350.00550.00180.00100.00
55.00100.00
Vitamins p–Amino benzoic acidD-(+)-BiotinD-Calcium pantothenateFolic acidmyo-InositolNicotinic acidPyridoxine x HClRiboflavinThiamine x HClVitamin B12
0.020.010.110.020.020.020.020.020.020.01
TNM-FH MediumDescriptionThe TNM-FH is a variation of the Grace medium. This modification has proved as a good culture medium for many lepidopteran cells.
Liquid Media
TNM-FH Medium(1)
with L-Glutaminewith Lactalbumine-Hydrolysatewith Yeast extractwith 0.35 g/L NaHC03 500 ml P04-80500
Special Media
TNM-FH Medium(2)
with L-Glutaminewith Lactalbumin-Hydrolysatewith Yeast extractwith 10 % Fetal Bovine Serumwith 0.35 g/L NaHCO3 500 ml P04-83500
Powder Media
TNM-FH Insect Medium(1)
without L-Glutaminewith Lactalbumine-Hydrolysatewith Yeast extract 10 L P03-9710without NaHCO3 50 L P03-9750
Components mg/L
Inorganic Salts
Calcium chloride x 2H2OPotassium chlorideMagnesium chloride x 6H2OMagnesium sulfate drieddi-Sodium hydrogen phosphate
1,324.622,240.002,278.861,939.80
876.92
OtherCompo-nents
DL-Malic acidSuccinic acidD-FructoseFumaric acidD(+)-Glucose anhydrousYeast extractα-Ketoglutaric acid sodium saltLactalbumin HydrolysateSucrose
670.0060.00
400.0055.00
700.003,333.33
425.66
3,333.3326,680.00
AminoAcids
β-AlanineL-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
200.00225.00700.00350.00350.00
19.18600.00600.00650.00
2,500.0050.0075.00
625.0050.00
150.00350.00550.00175.00100.00
50.00100.00
Vitamins p-Aminobenzoic acidD-(+)-BiotinD-Ca-PantothenateCholin chlorideFolic acidmyo-InositolNicotinic acidPyridoxol x HClRiboflavinThiamine x HCl
0.020.010.020.200.020.020.020.020.020.02
Composition
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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MediaMedia
Waymouth´s MB 752/1 MediumDescriptionWaymouth‘s MB 752/1 Medium was developed for studies concerning nutrition and metabolism. It also can be used for growing strain L sub-lines, NCTC clone 929.
Composition
When 4,766.40 mg/L HEPES is included there is only 5,500.00 mg/L sodium chloride.
Special Media
Waymouth’s MB 752/1 Medium(2)
with L-Glutaminewith 2.24 g/L NaHCO3 500 ml P04-28500
Powder Media
Waymouth’s MB 752/1 Medium(1)
with L-Glutamine 10 L P03-4510without NaHCO3 50 L P03-4550
Components mg/L
Inorganic Salts
Calcium chloride x 2H2OMagnesium chloride x 6H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium chloridedi-Sodium hydrogen phosphate anhydrous
120.02240.00130.96150.00
80.00
6,000.00300.00
OtherCompo-nents
D(+)-Glucose anhydrousGlutathione (red.)HepesHypoxanthinePhenol red
5,000.0015.00
4,766.4025.0010.00
AminoAcids
L-Arginine x HClL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-ThreonineL-TryptophanL-TyrosineL-Valine
75.0060.00
100.2615.00
350.00150.00
50.00164.10
25.0050.00
240.0050.0050.0050.0075.0040.0040.0065.00
Vitamins L-Ascorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12
17.500.021.00
250.000.401.001.001.001.00
10.000.20
Composition
When 5,958.00 mg/L HEPES is included there is only 6,300.00 mg/L sodium chloride.
Components mg/L
Inorganic Salts
Calcium chloride x 2H2OIron(III)-nitrat x 9H2OPotassium chlorideCopper(II)-sulfate x 5H2OMagnesium sulfate driedManganese chloride x 4H2OSodium chlorideSodium dihydrogen phospha-te x H2OZinc sulfate x 7H2O
264.920.0001400.000.0001139.570.0001
6,800.00140.00
0.0002OtherCompo-nents
D(+)-Glucose anhydrousHepesGlutathion (red.)MethyllinoleatSodium pyruvatePhenol red
2,000.005,958.00
0.050.03
25.0010.00
AminoAcids
L-AlanineL-Arginine free baseL-Asparagine x H2OL-Aspartic acidL-CysteineL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine
90.0050.0020.0030.0040.0020.00
292.0050.0050.0015.0050.0075.0087.5015.0025.0030.0010.0040.0010.0035.0050.00
Vitamins L-Ascorbic acidD(+)-BiotinCalciferolD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolMenadion sodium bisulfiteNikotinamidPyridoxal x HClRiboflavinThiamine x HClDL-α-Tocopherol phospha-te-Na2Vitamin A acetateVitamin B12
2.000.500.101.001.501.002.000.011.001.000.101.000.01
0.100.20
William´s Medium EDescriptionThe William‘s Medium E is used for long-term cultivation of adult rat liver epithelial cells.
Liquid Media
William’s Medium E(1)
without L-Glutaminewith 2.24 g/L NaHCO3 500 ml P04-29050
William’s Medium E(1)
with L-Glutaminewith 2.24 g/L NaHCO3 500 ml P04-29500
William’s Medium E(1)
with stab. Glutaminewith 2.24 g/L NaHCO3 500 ml P04-29150
William’s Medium E(1)
without L-Glutaminewithout Phenol redwith 2.24 g/L NaHCO3 500 ml P04-29510
Special Media
William´s Medium E(2)
without L-Glutaminewithout Glucosewith 2.24 g/L NaHCO3 500 ml P04-29050S1
Powder Media
William’s Medium E(1)
with L-Glutaminewith 25 mM Hepes 10 L P03-4810without NaHCO3 50 L P03-4850
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
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Media
Page 74
Endopan 3Large Vessel Endothelial Cell Medium
BackgroundEndothelial cells line the blood and lymphatic vessels and the internal cavities of the heart. They display a strongly flattened, polygonal form and mostly rest on a basal membrane. They adhere to each other by desmosomes and tight-junctions.
With a total cell number of about one trillion (1012), the endothelium is one of the biggest organs of the body and plays a key role in many physiological and patho-physiological processes (e.g. cell-based immune response, wound healing, inflammation, allergy, cardiovascular diseases, tumour growth). A huge number of soluble factors circulating in the blood or released by neighbouring cells, control proliferation or apoptosis of endothelial cells and the invasion and migration of leucocytes to the endothelium, thereby regulating the maintenance, degeneration, or regeneration of blood vessels.
The endothelium constitutes a highly specialized organ that lines the vascular system and lymphatic channels in a complex network of arteries, veins, and micro-vessels which differ in size, structure, and function. The cultivation of endothelial cells from large vessels, predominantly from human umbilical vein, is a routine procedure in many laboratories, and this has contributed huge to the development of modern vascular biology. However, there is convincing evidence that micro-vascular endothelial cells display a number of important functional differences, compared to large vessel-derived
CompositionEndopan 3 ready-to-use is a specially developed medium for the in vitro culture of human endothelial cells containing all components necessary for optimal growth. It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere.
Endopan 3 ready-to-use(1) 500 ml P04-00100Endopan 3 kit with 9 supplements(1) 500 ml P04-0010K
Endopan MVMicrovascular Endothelial Cell Medium
Endopan MV ready-to-use(3) 500 ml P04-00200Endopan MV kit with 8 supplements(3) 500 ml P04-0020K
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MediaMedia
Endopan PROEndothelial Progenitor Medium
BackgroundEndothelial cells line blood and lymphatic vessels and the internal cavities of the heart. They display a strongly flattened, polygonal form and mostly rest on a basal membrane. With a total number of about 1012 cells, the endothelium is one of the biggest organs of the body and plays a key role in many physiological and patho-physiological processes. A number of factors control proliferation or apoptosis of endothelial cells, thereby regulating the maintenance, degeneration, or regeneration of blood vessels.
New blood vessel formation occurs via angiogenesis or vasculogenesis, a process thought to be restricted to embryonic development. In 1997, postnatal vasculogenesis has been proposed as an important mechanism for angiogenesis via blood or bone marrow derived circulating progenitor endothelial cells (PEC) (Asahara et al, Science 1997). Consequently, PECs have been extensively studied as a potential cell therapy for the repair of damaged blood vessels. Animal studies clearly demonstrated that administration of PECs partially rescued cardiovascular dysfunction or myocardial injury with evidence for PEC contribution to new vessel growth.In most studies, PECs are defined by cell surface expression of CD34, CD133, or VEGF-R2 (KDR). Because these molecules are also present on hematopoietic progenitors, relying only on surface markers can not exclude a contamination with hematopoietic linage cells. More recently, a PEC population has been identified which shows expression of endothelial as well as progenitor, but not hematopoietic cell markers (Ingram et al, Blood. 2004;104:2752). Importantly, these cells have been tested for a high proliferative potential in clonogenic assays and additionally characterized by formation of functional blood vessels in vivo (Yoder et al, Blood. 2007;109:1801).
CompositionEndopan PRO ready-to-use is a complete medium specially developed for the in vitro culture of human progenitor endothelial cells (hPEC) containing all components necessary for optimal colony formation, clonogenic growth, and rapid proliferation.
Endopan PRO kit is provided with FBS growth supplement (pre-screened and tested for progenitor cells) and additional supplements in separate sterile packing. This will enable the user to prepare a medium for special application.
Endopan PRO ready-to-use(3) 500 ml P04-00700Endopan PRO kit with 6 supplements(3) 500 ml P04-0070K
endothelial cells, with regard to their growth factor response and their regulation of adhesion molecule expression.
They serve as the barrier separating circulating blood from the extracellular matrix and interstitium in the body. Cells involved in the pathogenesis of tumor angiogenesis, wound healing, and acute or chronic inflammation are predominantly of micro-vascular origin. Several functions associated with the micro-vasculature in situ are expressed by micro-vascular endothelial cells in cell culture.
Micro-vessels are not simply tubes but have also a second cellular component, the mural cell or pericyte. Little is known about later stages of vessel growth, including the addition of pericytes to the capillary and its influence on endothelial growth and function. In vivo, pericytes form an incomplete envelopment around the endothelial cells within the micro-vascular basement membrane of capillaries and post-capillary venules.
Evidence clearly indicates that differences exist between endothelial cells of the microvasculature and those that line large vessels. These include differences in secreted products, in the expression of cell adhesion molecules, and in cytokine-induced regulation of cell adhesion molecules. Thus, a precise delineation of the biology of micro-vascular endothelial cells is crucial to our understanding of such important processes as inflammation, tumor progression, cardiac microcirculation, and blood-brain barrier function.
Endopan
Endopan 3 kit is provided with FBS and supplements in separate sterile packing. This will enable the user to prepare a medium for special application. For example, FBS, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings.
hPEC colony (P1) with outgrowing cells in Endopan PRO
hPEC in Endopan PRO (P6)
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
NEWNEW
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Media
Page 76
HepatopanHuman Hepatocyte Medium
DescriptiomLike every human organ, the liver consists of a complicated compound of different cells with varying functions. Hepatocytes represent – in terms of figures – with 75 % of the total number of liver cells the most important component. The metabolism, this means the chemical transformation of almost all substances which are taken in by the body, takes place in the liver.
CompositionThe hepatocyte medium from PAN-Biotech is supplied as a basal medium with four supplements (storage of the supplements at -20° C). The supplements have to be ad-ded to the medium before use. The medium does not con-tain fetal bovine serum.
Hepatopan with 4 supplements(3) 500 ml P04-00600
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MediaMedia
MelanopanMelanocyte Medium
DescriptionMelanocytes are embedded in the basal and spike cell lay-er of the epidermis. They produce the pigment melanin and take care of the protective function of the skin against UV damages. If the solar radiation is too strong, the me-lanocytes are damaged and can develop into tumour cells.
CompositionThe melanocyte growth medium from PAN-Biotech is supplied as a basal medium with seven supplements (storage of the supplements at -20° C). The supplements have to be added to the medium before use. The medium does not contain fetal bovine serum.
Melanopan with 7 supplements(3) 500 ml P04-740500
StempanES-Cell Medium
DescriptionStem cells are non-specialized cells with the ability (potency) to develop into different organo-typic cell types (e. g. heart, nerve, blood, muscle and cartilage cells). Depending on their origin, they are divided into embryonic and adult stem cells.
CompositionFor the cultivation of embryonic stem cells, PAN-Biotech has developed a complete ready-to-use medium. The medium contains fetal bovine serum.
Stempan DMEM(2)
with L-Glutaminewith 3.7 g/L NaHCO3without LIF
500 ml P08-50500
Stempan GMEM(2)
with L-Glutaminewith 2.75 g/L NaHCO3without LIF
500 ml P08-50600
EMEM FibroblastsFibroblast Medium
DescriptionBased on EMEM, this medium was supplemented with amino acids and vitamins and optimized for an improved growth of fibroblasts.
EMEM Fibroblasts(1) 500 ml P04-08049
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
Neuropan-Basal Media (Basicmedia)(2) 500 ml P04-00900
Neuropan 27 supplement 20x(2) 100 ml10 ml
P07-07100P07-07010
Neuropan 27 supplement 50x(2) 100 ml10 ml
P07-07200P07-07210
Neuropan 27 supplement 20xwithout Antioxidant(2)
100 ml10 ml
P07-10100P07-10010
Neuropan 2 supplement 100x(2) 100 ml10 ml
P07-11100P07-11010
NS21 Supplement 50x sterile(3) 10 ml P07-20021NS21 Supplement 50x non-sterile(3) 10 ml P07-20001
NeuropanNeuronal Cell Medium
Neuropan 27 is a concentrate for the serum-free cultivation of neural cells.
NS21 SupplementTo culture neurons in the absence of serum, defined supplements such as B27 are widely used. However, available supplements exhibit some variability in their capability to support neurons in culture. NS21 Supplement is a newly developed serum substitute for neuronal cultures of cells from the central and peripheral nervous system.
Neuropan Basal MediumNeuropan basal medium supports the growth of hippocampus cells and many other neuronal cells of the central nervous system. A feeder layer of astrocytes is not required. Neuropan basal medium does not contain glutamate which should be added for the initial culture (25 µM). Before use, Neuropan basal medium is supplemented with serum or for a serum-free culture with Neuropan 27 or NS21 Supplement.
Pantum
Pantum 386(3) 500 ml P04-00386
Pantum 586A(3) 500 ml P04-00586
Pantum L24(3) 500 ml P04-00024
Pantum T64(3) 500 ml P04-00064
DescriptionPantum are ready-to-use growth media which contain purified plasma proteins and lipids, such as serum albumin and cholesterol, specific growth factors, components of soybean extract, an iron transport protein and enriched trace elements. The new formulations result in stable cell growth under defined culture conditions. No addition of serum or growth factors is necessary.
Pantum 386 for epithelial cellsPantum 386 is a modified formulation of DMEM and specially developed to optimize the growth of epithelial cell lines.
Pantum 586A for adherent cellsPantum 586A is particularly suited for culture of adherent cells to stimulate their growth. It is a modification of Iscove’s MEM.
Pantum L24 for lymphocytesPantum L24 is suited for culture of peripheral blood lymphocytes. Adult lymphocytes lack the ability to proliferate. Therefore, Pantum L24 contains a mitogen (phytohemagglutinin, PHA) specifically acting on the cell cycle. It is a modified formulation of RPMI 1640.
Pantum T64 for tumor cellsPantum T64 is specially developed for culture of tumor cells to stimulate the growth of this cell type. It is a modification of RPMI 1640.
For the cultivation of fibroblasts, this medium has to be supplemented with 10% FBS before use.
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Media
Page 78
AmniopanPrenatal Cytogenetics Medium
DescriptionAmniopan is a complete ready-to-use medium intended for in vitro diagnostic use with a short term culture of human fetal cells from amniotic fluid or chorion villi biopsy (CVS) material for a standardized application in cytogenetic studies.Amniopan is intended for in vitro use and has been de-signed for establishing cultures of human fetal cells from amniotic fluid or chorion villi biopsies (CVS), which then can be used in karyotyping, fluorescence in-situ hybridisation (FISH) or other cytogenetic procedures.The media formulation of Amniopan was optimized on human fetal cells from amniotic fluid and CVS, with special emphasis on fast attachment of cells to the cell culture substrate and efficient cell growth to facilitate rapid diagnostic findings.
CompositionAmniopan is supplied frozen as a complete medium, ready-to-use in a 100 ml format. It is based on al-pha-MEM and contains antibiotics, L-glutamine, foetal bovine serum (FBS), hormones and growth factors.
SuitabilityAmniopan is a complete medium (ready-to-use) for the cultivation of human fetal cells from amniotic fluid and chorion villi biopsy samples. It is suitable for a rapid ex-pansion of amniotic cells in order to investigate chromo-somal disorders. The number and quality of metaphases in Amniopan are significantly higher and independent of individual batches as compared to other media.
Amniopan(1) 100 ml P04-70100
Amniopan S2Prenatal Cytogenetics Medium
DescriptionAmniopan S2 is a complete ready-to-use medium inten-ded for in vitro diagnostic use with a short term culture of human fetal cells from amniotic fluid or chorion villi biopsy (CVS) material for a standardized application in cytogenetic studies.Amniopan S2 is intended for in vitro use and has been designed for establishing cultures of human fetal cells from amniotic fluid or chorion villi biopsies (CVS), which then can be used in karyotyping, fluorescence in-situ hybridisation (FISH) or other cytogenetic proce-dures. The media formulation of Amniopan S2 was further optimized on human fetal cells from amniotic fluid and CVS, with special emphasis on fast attachment of cells to the cell culture substrate and efficient cell growth to facilitate rapid diagnostic findings.
CompositionAmniopan S2 is supplied frozen as a complete medi-um, ready-to-use in a 100 ml format. It is based on alpha-MEM and contains antibiotics, L-glutamine, fetal bovine serum (FBS), hormones and an increased amount of growth factors.
SuitabilityAmniopan S2 is a ready-to-use medium for the cultivati-on of human fetal cells from amniotic fluid and chorion villi biopsy samples. It is suitable for a rapid expansion of amniotic cells in order to investigate chromosomal disorders. The number and quality of metaphases in Amniopan S2 are significantly higher and independent of individual batches as compared to other media.
Amniopan S2(1) 100 ml P04-70101
www.pan-biotech.com Page 79
MediaMedia
MarrowpanMarrow Cell Medium
DescriptionMarrowpan is a complete ready-to-use medium inten-ded for in vitro diagnostic procedures with a short term culture of bone marrow and other hematopoietic cells for cytogenetic studies.Marrowpan is intended for in vitro use and has been designed for establishing cultures of bone marrow and leukemic blood cells, which then can be used in karyoty-ping, fluorescence in-situ hybridisation (FISH) or other cytogenetic procedures.Marrowpan can be used as a neutral medium to culture different haematopoietic cells (myeloid and lymphoid lineages) present in bone marrow or leukemic blood samples. Marrowpan is also used together with a mito-gen specific for B or T lymphocytes where these particu-lar lineages are being investigated.
CompositionMarrowpan is supplied frozen as a complete medium, ready-to-use in a 100 ml format. It is based on al-pha-MEM and contains antibiotics, L-glutamine, fetal bovine serum (FBS), hormones and growth factors.
SuitabilityMarrowpan is a complete medium (ready-to-use) for the cultivation of cells from peripheral blood or bone marrow. It is suitable for a rapid expansion of blood cells in order to investigate leukemic diseases (e.g. ALL, AML, CLL, CML, MPN, MDS). The number and quality of metaphases in Marrowpan are significantly higher and independent of individual batches as compared to serum-containing media.
Marrowpan(1) 100 ml P04-70200
Marrowpan S2Marrow Cell Medium
DescriptionMarrowpan S2 is a complete ready-to-use medium intended for in vitro diagnostic procedures with a short term culture of bone marrow and other hematopoietic cells for cytogenetic studies. Marrowpan S2 is intended for in vitro use and has been designed for establishing cultures of bone marrow and leukemic blood cells, which then can be used in karyotyping, fluorescence in-situ hybridisation (FISH) or other cytogenetic proce-dures.Marrowpan S2 can be used as a neutral medium to culture different haematopoietic cells (myeloid and lymphoid lineages) present in bone marrow or leukemic blood samples. Marrowpan S2 is also used together with a mitogen specific for B or T lymphocytes where these particular lineages are being investigated.
CompositionMarrowpan S2 is supplied frozen as a complete me-dium, ready-to-use in a 100 ml format. It is based on alpha-MEM and contains antibiotics, L-glutamine, Foetal Bovine Serum (FBS), hormones and an increased amount of growth factors.
SuitabilityMarrowpan S2 is a ready-to-use medium for the culti-vation of cells from peripheral blood or bone marrow. It is suitable for a rapid expansion of blood cells in order to investigate leukemic diseases (e.g. ALL, AML, CLL, CML, MPN, MDS). The number and quality of metaphases in Marrowpan S2 are significantly higher and independent of individual batches as compared to serum-containing media.
Marrowpan S2(1) 100 ml P04-70201
3 3
(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request
NEW
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Index
Alphabetical Product Index Alphabetical Product Index
Product PageAAccutaseAgaroseAlbumin, bovineAlbumin, humanAlpha MEMAmino AcidsAmniopanAmniopan S2Amphotericin BAnimal SerumAntibioticsAntifungal DrugsAttachment Factors
100131104104
3897767698119898
102
BBacitracin BBarrycidal BarrydinBiologicalsBME SolutionBME VitaminsBME with Earle´s SaltsBME with Hank´s SaltsBovine SerumBovine Serum Albuminβ-Mercapthoethanol
98110111115
97974040
5104
93
CChemokinesCMRL-1066 MediumCollagen ACollagen RCollagenaseCryo PreservationCryopan
12041
102102100108108
DDemecolcin SolutionDesipureDimethylsulfoxideDisinfectantsDispaseDMEM/F12 MediumDMSOdNTP MixdNTP SetsDulbecco´s Modified Eagle MediumDulbecco´s Phosphate Buffered Salt Solution
93112108109101
46108133132
42
94
Product PageEEarl´s Buffered Salt SolutionEDTAEMEM Fibroblast MediumEndopan 3Endopan 300 SLEndopan MVEndopan PROEnzymes for Cell Dissociation
94101
7572347273
100
FFBSFBS BiotechFBS GoodFibronectinFreezing Medium
565
103108
GGelatine SolutionGentamycin SulphateGrey´s Balanced Salt SolutionGlasgow MEM (BHK 21)Grace´s Insect MediumGrowth Factors
10298964849
116
HHam´s F10 MediumHam´s F12 MediumHank´s Balanced Salt SolutionHAT SupplementHT SupplementHepatopanHepes - Sodium SaltHepes BufferHuman CytokinesHuman SerumHuman Serum AlbuminHuman Transferrin apoHygromycin B
5051959393749393
11612
1049398
IInsulin HumanIPL-41 Insect MediumIscove´s Modified Dulbecco´s MediumITS Solution
935452
93
JJoklik-MEM 55
KKanamycin Sulphate 98
Product PageLLaminin MouseLeibovitz´s L-15 MediumL-Glutamine
1035697
MMarrowpanMarrowpan S2Mc Coy´s 5A MediumMCDB MediumMedia SupplementsMedium 199 with Earle´s SaltsMedium 199 with Hank´s SaltsMelanopanMEMMEM Vitamine SolutionMEM with Earle´s SaltsMEM with Hank´s SaltsMinocyclinMolecular Biology Mycorase
77775758935960749797616398
12399
NNeomycin SulphateNeuropanNystatin Solution
987598
PPAN DNA CleanPAN Hot Start DNA-PolymerasePAN Ladder IPAN SL-S Product LinePancollPanserin T3Paneticin 420Panexin BMMPanexin NTAPanexin NTSPanexin SL-SPANScript DNA-PolymerasePANScript Red DNA-PolymerasePansera ESPanserin 293APanserin 293SPanserin 401Panserin 411Panserin 411SPanserin 412Panserin 413Panserin 416Panserin 701Panserin 801
130126129107105
2899201819
107124125
727272122222323232929
Product PagePPanserin C6000Panserin H4000Panserin H8000Panserin ProVeroPanserin PX10Panserin PX40Panserin S2Panserin T3PANsys 3000PantumPenicillinPluronicPolymerasesPolymycin BPowerScript DNA-Polymerase Long RangePowerScript DNA-Polymerase Short RangePowerStem ESPro 1PowerStem ESPro2PowerStem ESTPowerStem HE1PowerStem HE2PowerStem HPSCPowerStem iPS1PowerStem iPS2PowerStem MSC1PowerStem PEC1 PowerStem PEC1 kitProteinase KPuck´s Salt Solution A
2624252830313328
135749993
12399
128
127
8182838485898687889090
12996
RRPMI 1640 64
SSchneider´s Drosophila MediumSeparating SolutionsSeparating Solutions Pre-FilledSL-S CollagenSL-S CryopanSL-S MediumSL-S TrypsinSodium BicarbonateSodium ChlorideSodium PyruvateSpodopanStempanSterile WaterStreptomycin
67105106107107107107
93939332759399
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Index
Alphabetical Product Index
Product PageTTC 100 Insect MediumTiamulinTNM-FH MediumTreated SerumTryode´s Salt SolutionTrypsinTryptose Phosphate
6899691096
10193
VVitamins 97
WWaymouth´s MB 752/1 MediumWilliam´s Medium E
7071
ZZeocin 99
Numeric Product Index
Product No. Page Product No. Page Product No. Page Product No. Page36000036005036010136040036100036500046500066000070050070100071050072050072050173001074001027050052705001SCB-11000050CB-1101001CB-1101002CB-1101003CB-1102010CB-1102011CB-1102021CB-1102023CB-1102024CB-1104112CB-1104113CB-1104201CB-1104202CB-1105001CB-1106001CB-1108002CB-1108003CB-1108010CB-1108011CB-1108100CB-1109200CB-1109201CB-1109301CB-1110000CB-1110001CB-1110002CB-1111122CB-1111123CB-1111131CB-1111151CB-1111153CB-1111162
109109109109109109109109109109109109109109109103103119116116116116116116116116116116116116117117116116116116116118118118118118118118118118118118118
CB-1112011CB-1112012CB-1113008CB-1113011CB-1113012CB-1114002CB-1114011CB-1114013CB-1114100CB-1114102CB-1115000CB-1115001CB-1115002CB-1115006CB-1116001CB-1117001CB-1117001MCB-1117007CB-1117008CB-1118005CB-1118008CB-1119000CB-1119001CB-1122001CB-1125032CB-1127000CB-1127100CB-1200000CB-1210000CB-1212011 CB-1212011MCB-1214000CB-1214001CB-1214120CB-1214121CB-1232701CB-1300058CB-1300118CB-1407003CB-1412011CB-1515001CB-2015001CB-2110000CB-2110002CB-2110003CB-2110005CB-2110100CB-2110101CB-2120100
118118116116116118118118118118116116116116116117117119119120120116116120117118118119119119119119119119119120117118120119116116116116116116116116117
CB-2120101CB-2120110CB-2120112CB-2120121CB-2130120CB-2130121CB-2130123CB-2130202CB-2130203CB-2130300CB-2130301CB-2130405CB-2130407CB-2130501CB-2130600CB-2130603CB-2131000CB-2131001CB-2131201CB-2131500CB-2131501CB-2210000CB-2210001CB-2210002CB-2230030CB-2230031CB-2230111CB-2230120CB-2230121CB-2230220CB-2230221CB-2230300CB-2230301CB-2230302CB-2230403CB-2230600CB-2230601CB-2230602CB-2231202CB-2232000CB-2232002CB-2250001CB-2310015CB-2310016CB-2330000CB-2331001CB-2420031CB-2420032CB-2430122
117117117117117117117117117117117117117117117117117117117117117119119119119119119119119119119119119119119119119119119120120119119119119119119119119
CB-2800001CB-2800002CB-2800007CB-2800008CB-2800010CB-2800011CB-3410010CB-3410011CB-4070010LS0002100LS0004174LS0004176LS0004180LS0004182LS0004186LS0004188LS0004194LS0004196MB-1100500MB-1100600MB-1120250MB-1120500MB-1860250MB-1860500MB-1865000MB-30010250MB-30020250MB-4300002P03-0010P03-0050P03-01510P03-01550P03-02010PP03-02050PP03-0510P03-0550P03-0710P03-0750P03-0810P03-0850P03-0910P03-0950P03-1010P03-1050P03-1110P03-1150P03-1210P03-1250P03-1310
116116116116116116118118117101100100100100100100100100124125128128126126126123123129
424242425555424245454545454545454747474753
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Index
Numeric Product Index
Product No. Page Product No. Page Product No. Page Product No. PageP03-1350P03-1410P03-1450P03-1510P03-1550P03-1610P03-1650P03-1710P03-1750P03-1810P03-1850P03-1910P03-1950P03-2110P03-2150P03-2310P03-2350P03-2410P03-2450P03-2510P03-2550P03-2610P03-2650P03-2710P03-2750P03-2810P03-2850P03-2910P03-2950P03-3010P03-3050P03-3110P03-3150P03-3310P03-3350P03-3910P03-3950P03-4010P03-4050P03-4110P03-4150P03-4310P03-4350P03-4410P03-4450P03-4510P03-4550P03-4810P03-4850
53535356565656575757575959606039393939393939396262626262626262484863635050505051516666656570707171
P03-5010P03-5050P03-5210P03-5250P03-6010P03-6050P03-6110P03-6150P03-6510P03-6550P03-6810P03-6850P03-6910P03-6950P03-7210P03-7250P03-7310P03-7350P03-7410P03-7450P03-7610P03-7650P03-7710P03-7750P03-9010P03-9050P03-9110P03-9150P03-9210P03-9250P03-9310P03-9350P03-9610P03-9650P03-9710P03-9750P04-00024P04-00064P04-00100P04-0010KP04-00200P04-0020KP04-00386P04-00507P04-00508P04-00509P04-00586P04-00600P04-00650
50505353474747474545484848486565666661616666656549494949545467676868696974747272727274616262747434
P04-0065KP04-00700P04-0070KP04-00900P04-01158P04-01159P04-01161P04-01163P04-01500P04-01501P04-01515P04-01516P04-01548 P04-01548S1P04-01549P04-01550P04-01551P04-01555P04-01597P04-02500P04-02500S1P04-02501P04-03500P04-03503P04-03510P04-03550P04-03551P04-03556P04-03560P04-03588P04-03590P04-03591P04-03596P04-03598P04-03600P04-04057P04-04500P04-04510P04-04550P04-05050P04-05500P04-05540P04-05545P04-05550P04-05551P04-05610P04-06500P04-07050P04-07150
34737375434343444243424342424242424344424343434444434443444443434344434443434457574444444257575959
P04-07250P04-07450P04-07500P04-07600P04-07753P04-08049P04-08050P04-08056P04-08150P04-08250P04-08500P04-08509P04-08510P04-08549P04-09050P04-09500P04-10050P04-10500P04-10599P04-12049P04-12050P04-12500P04-13050P04-13500P04-14500P04-14501P04-14550P04-14559P04-15500P04-15600P04-16151P04-16500P04-16515P04-16516P04-16520P04-16530P04-16598P04-17500P04-17510P04-17545P04-17546P04-17550P04-17598P04-17599P04-17850P04-18000P04-18047P04-18050P04-18500
59605960607561626162626162626162636363505050505051515151515165666664666666646566666566656564666666
Numeric Product Index
Product No. Page Product No. Page Product No. Page Product No. PageP04-19500P04-19550P04-20050P04-20150P04-20150S2P04-20250P04-20259P04-20350P04-20351P04-20450P04-20451P04-20451S1P04-21049P04-21050P04-21051P04-21060P04-21150P04-21200P04-21250P04-21300P04-21350P04-21500P04-21502P04-22100P04-22500P04-25050P04-25500P04-26050P04-27050P04-27054P04-27055P04-27500P04-28500P04-29050P04-29050S1P04-29150P04-29500P04-29510P04-30010PP04-300500P04-30050PP04-30500P04-31500P04-32010PP04-32050PP04-32100P04-32105P04-32500P04-32505
656652525352535253535353653838383855385538383866654040405656565670717171717194
107949494959595959595
P04-33010PP04-33050PP04-33100P04-33500P04-34100P04-341000P04-34500P04-35500P04-360010BP04-36050PP04-361000P04-362500P04-3625CP04-36500P04-3650CP04-37500P04-38500P04-39500P04-41150P04-41151P04-41250P04-41251P04-41252P04-41450P04-41500P04-41550P04-41650P04-43100P04-44100P04-47500P04-48010PP04-48050PP04-48500P04-49100P04-49105P04-49500P04-49505P04-50100P04-50105P04-50500P04-50505P04-51100P04-51500P04-53500P04-54010PP04-54050PP04-54500P04-60100P04-60125
9595959595959494949494949494949494474747474747474747939394969696969595959595959595969694969696
105106
P04-60225P04-60500P04-63100P04-63225P04-63500P04-64100P04-64500P04-65100P04-65500P04-67100P04-67500P04-68100P04-68500P04-70100P04-70101P04-70200P04-70201P04-710100P04-710110P04-710200P04-710210P04-710401P04-71040MP04-710411P04-710411MP04-710412 P04-710412MP04-710413P04-710416P04-710608P04-710608MP04-710609 P04-710609MP04-710613 P04-710613MP04-710701 P04-710701MP04-710801P04-710PX10P04-710PX40P04-714000 P04-714000MP04-71411SP04-716000 P04-716000MP04-718000 P04-718000MP04-740500P04-7411S0
106105105106105105105105105105105105105
767677772828333321212222232323232727272728282929293031242422262625257422
P04-7411S1P04-77010KP04-7701KP04-77020KP04-7702KP04-77110KP04-7711KP04-77120KP04-7712KP04-77130KP04-7713KP04-77140KP04-7714KP04-77210KP04-77250KP04-77310KP04-77350KP04-77410KP04-77450KP04-7751P04-77510KP04-77620KP04-7762KP04-777500P04-77750KP04-80025PP04-80050P04-80053P04-80054P04-80057P04-80100P04-80100PP04-80500P04-80500PP04-81500P04-82010PP04-82050P04-82100P04-82500P04-83500P04-84500P04-84600P04-850100P04-850500P04-85600P04-90065SP04-90500P04-91500P04-92500
228181828284848585868687878383888889898181828290909797585858979769974997979749694141323254
107676768
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Numeric Product Index
Product No. Page Product No. Page Product No. Page Product No. PageP04-93500P04-951SA2P04-95700P04-95750P04-95800P04-95850P04-96500P04-97500P04-98500P04-991000P04-991500P04-992000P05-01100P05-01100PP05-01500P05-01500PP05-39500P06-01001P06-01005P06-01050P06-01050PP06-01100P06-01100PP06-01225PP06-01250PP06-02010PP06-02025PP06-02100P06-03001P06-03001PP06-03004P06-03005P06-03010PP06-03025PP06-03050P06-03100P06-04001P06-04005P06-04010PP06-04050P06-04050PP06-04100P06-05050P06-05100P06-06010PP06-06025PP06-06050P06-06100P06-06100P
68201818191948484893939393939393939898989898989898989898989898989898989898989898989898989898989898
P06-07001P06-07005P06-07050P06-07100P06-07300P06-07301P06-07305P06-07350P06-07800P06-080050PP06-080100PP06-08020P06-08025PP06-08100P06-08100PP06-0849010P06-0849050P06-09050P06-11025PP06-11050PP06-11100PP06-12001P06-12005P06-12050P06-12100P06-13001P06-13005P06-13050P06-13100P06-138110P06-138150P06-138210P06-138250P06-138310P06-138350P06-138410P06-138450P06-1391050P06-1391100P06-1391500P06-139210P06-139250P06-139310P06-139350P06-139410P06-139450P06-14001P06-14005P06-14050
999999999999999998989898999899
104104
999999999999999998989898
104104104104104104104104104104104104104104104104104
989898
P06-14100P06-15001P06-15005P06-15050P06-15100P06-16001PP06-16005PP06-16010PP06-16020P06-16100P06-16220P06-20020P06-20030P06-20100P06-20166P06-20410P06-20501P06-20650P06-21000P06-21100P06-21500P06-25200P06-26025P06-26050P06-28010P07-01100P07-02100P07-03100P07-03110P07-03200P07-03210P07-03300P07-03310P07-03400P07-03410P07-04100P07-04200P07-04300P07-05020P07-05100P07-07010P07-07100P07-07200P07-07210P07-10010P07-10100P07-11010P07-11100P07-20001
9898989898999999999999
102102102102102103107
939393
102104104
99939393939393939393939393939393757575757575757575
P07-20021P07-90050P07-91010P07-92010P07-92050P07-94050P08-02100P08-2000P08-30100P08-31100P08-32100P08-40100P08-41100P08-50500P08-50600P10-019100P10-019500P10-020100P10-020500P10-021100P10-021500P10-022100P10-022500P10-023100P10-0231SFP10-0231SPP10-023500P10-0235SPP10-024100P10-024500P10-025025PP10-025100P10-025100PP10-025500P10-025500PP10-026100P10-026500P10-027100P10-027500P10-028100P10-029100P10-029500P10-031100P10-032100P10-033100P10-0331SFP10-038100P10-038500P10-040100
75108
93108108107
939797979797977575
101101101101101101101101101107101101101101101101101101101101101101101101101101101
93101101107101101101
Numeric Product Index
Product No. Page Product No. Page Product No. Page Product No. PageP10-040500P10-100100P10-21100P-2060020P-2060100P-2061000P-2360011P-2360012P-2360013P-2701001P-2701002P30-0101P30-0102P30-01901P30-01901EP30-0200P30-0201P30-0210P30-0301P30-0302P30-0401P30-0402P30-0601P30-0602P30-0701P30-0702P30-0801P30-0802P30-0901P30-0902P30-1001P30-1002P30-1101P30-1102P30-1301P30-1302P30-1401P30-1402P30-1501P30-1502P30-1505P30-1506P30-1701P30-1702P30-1905P30-1906P30-1908P30-1909P30-1981
101101100117117117117117117116116
111111111111111111
5555
11111111111111111111
5555555555
1010101010
P30-1985P30-2008P30-2009P30-2081P30-2085P30-2101P30-2102P30-2301P30-2302P30-2401P30-2402P30-2501P30-2502P30-2601P30-2602P30-2605P30-2606P30-2608P30-2609P30-2701P30-2702P30-2801P30-2802P30-2901P30-2902P30-3301P30-3302P30-3305P30-3306P30-3401P30-3402P30-3601P30-3602P30-4101P30-4102P30-5100P30-5500P30-8100P30-8500P-3340002P-3610002P-3860001P-3860002P40-1301P40-1302P40-1401P40-1402P40-37100P40-37500
10101010101010101012121212
777777
121210101212
5555
101010101111
5555
120116119119
777766
P40-38100P40-38500P40-39100P40-39500P40-58100P40-58500P40-59100P40-59500P-4390002P-4390010P60-15840100P60-36720100PAN721064PAN721065PAN733025PAN733026PAN737042PAN737046PAN739025PAN739026PAN739028PAN739043PAN741025PANsys3000
66666666
119119108108127127129129130130132132133133131135
PAN-Biotech GmbHAm Gewerbepark 13D-94501 Aidenbach
GERMANY
Phone: +49 (0)8543/6016-30Fax: +49 (0)8543/6016-49
www.pan-biotech.dewww.pan-biotech.com
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