Post on 05-Dec-2014
description
Doc. Simone Bellassai
R & D Chemist
s.bellassai@cdr-mediared.it
WineLab: fast, simple and reliable wine testing system
CDR s.r.l.
CDR, development and engineering company
The fields of application of CDR systems are:
Telematics: automatic tools for motorway stations and parking places
Medical diagnostics: hematology, hemostasis and veterinary systems
Food diagnostics: instruments and reagents for rapid, accurate and safe food analysis
Wine diagnostics
WineLab
Wine analysis
Must analysisGrapes analysis
WineLabAntocyanes
Free SO2
Total SO2
Malic Acid
Lactic Acid
Glucose and FructoseAlcohol Content
pH
Total acidity
Volatile acidity
Polyphenols
Y.A.N.
Wine diagnostics
System overview
WineLab system is constituted by:
• A thermostated analyzer based on photometric technology which uses solid-state emitters
• Kits with pre-filled cuvettes and reagents, developed by our research laboratory, ready to use
• Pipettes• Print Manager, a software which enables to connect
the analyzer to the PC • User's manual• Illustrated data sheets• 3 years warranty
Features of the analyzer
• System: 4 wavelenghts and 12 incubation cells designed to heat samples at a temperature of 37°C
• Measurement: based on the reading of absorbance
• Photometric range: 0.0-2.3 Abs; (0.0-4.0 Abs) ampl.
• Hardware and Software: designed and manufactured by CDR S.r.l.
• Maintenance: not necessary periodic calibration
Tests
Tests
Competitors matrix
WineLab NIR External Laboratory
TraditionalSystems
Simplicity -
Analyzer price
Analysis price
Analysis speed
Calibration Not required Required - Required
- -Very HighLow
MediumMedium Low
High High Very Low
Medium
LowMedium High Low
Very Important!!
ISVEA validation
● CdR – WineLab system is validated by ISVEA
● This system doesn't need calibration. It is already calibrated and well correlated to the official methods
ISVEA validation
Acido Acetico (acidità volatile) su vino
R2 = 0,9709
0
0,2
0,4
0,6
0,8
1
1,2
1,4
0 0,2 0,4 0,6 0,8 1 1,2 1,4
WineLab (g/l ac. Acetico)
OIV
(g
/l a
c. A
ce
tic
o)
R2=0,97
R2=0,97
Volatile acidity (acetic acid)
Sugars
Acido L-Malico su vino
R2 = 0,9837
0
0,2
0,4
0,6
0,8
1
1,2
1,4
1,6
1,8
2
0 0,5 1 1,5 2
WineLab (g/l ac. Malico)
HP
LC
(g
/l ac.
Malic
o)
Malic Acid
R2=0,98
Glucose and fructose in wine
Fructose in wine
R2 = 0,9755
0
1
2
3
4
5
6
7
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0
WineLab (g/l fructose)
HP
LC
(g
/l f
ruc
tos
e)
Glucose in wine
R2 = 0,9704
0
0,5
1
1,5
2
2,5
3
3,5
0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5
WineLab (g/l glucose)
HP
LC
(g
/l g
luc
ose
) R2=0,97R2=0,97
Glucose + ATP + NAD+ ADP + gluconate-6-P + NADH HK + G6P-DH
HK + G6P-DH + PGIFructose + ATP + NAD+ ADP + gluconate-6-P + NADH
Fructose in wine Glucose in wine
● Determination of assimilable sugars! Pentose sugars are excluded
● You have a detection limit of 0.1 g/L! You can know with high precision, in the alcohol fermentation, when the sugars are finished, to avoid any risk of acetic acid.
● You can do up to 30 analysis/hour.
CdR-WineLab's advantages
Glucose and fructose in wine
Free sulfur dioxide
Step 1: taking and incubation of the sample
Free sulfur dioxide
Step 2: addition of the R2 and reading of the sample
Inorganic Nitrogen(ammonium)
Organic nitrogen (α-aminic)
Yeast Assimilable Nitrogen
OHO
O
OHO
N+
H
H
HH
NADH
OHO
NH2
OHO
Gl-DHNAD
+
keto-glutarate
Ammonium
Glutammate
NH2
R O
OH
O
O
ONH
SH
OH
CH3
O
Amino acid OPA NAC isoindolic derivative
NR
OOH
S
R
Yeast Assimilable Nitrogen
● You can check separately the amount of inorganic nitrogen and organic nitrogen.
● The test is not affected by proline and hidroxy-proline interference.
● You can do up to 30 tests/hour.
CdR-WineLab's advantages
User Advantages
Small and medium wineries
•carry out independently the most important analysis on wine•affordable analyzer price•lower test price compared to laboratories
Big wineries
•carry out analysis on a small samples quantity without using expensive and complicated automatic analysis systems
Enological consultants•check the most important parameters during wine production in the wineries•create a “portable laboratory”
Thanks to its simplicity and reliability, WineLab can be used by:
Users
The NEXT NEW TEST
● It is available by the beginning of june
● The polyphenol index is a reading of absorbance at 280nm. So you can do this without using an UV lamp and without quarz cuvettes
● You can use this test in the mixture of wines to control and keep the right polyphenols level
TOTAL POLYPHENOL INDEX
WineColor
• WineColor is a complementar analyzer to do Colour Intensity and Tonality reading the absorbance at 420, 520, and 620 nm
• You can do these analysis without dilution of the sample
• You can control and correct the colour of the wine
Summarizing....
● WineLab is a rapid simple and reliable system. This analyzer is able to control each step of the vinification from grapes to wine in bottle
● The winery is autonomous for the analysis and you can have a result in real time without waiting for the result from external laboratory
● WineLab gives an enological consultant the opportunity to work without opening a laboratory
CdR- WineLab
Thanks for your attention
Doc. Simone Bellassai
R & D Chemist
s.bellassai@cdr-mediared.it