Doc. Simone Bellassai

R & D Chemist

s.bellassai@cdr-mediared.it

WineLab: fast, simple and reliable wine testing system

CDR s.r.l.

CDR, development and engineering company

The fields of application of CDR systems are:

Telematics: automatic tools for motorway stations and parking places

Medical diagnostics: hematology, hemostasis and veterinary systems

Food diagnostics: instruments and reagents for rapid, accurate and safe food analysis

Wine diagnostics

WineLab

Wine analysis

Must analysisGrapes analysis

WineLabAntocyanes

Free SO2

Total SO2

Malic Acid

Lactic Acid

Glucose and FructoseAlcohol Content

pH

Total acidity

Volatile acidity

Polyphenols

Y.A.N.

Wine diagnostics

System overview

WineLab system is constituted by:

• A thermostated analyzer based on photometric technology which uses solid-state emitters

• Kits with pre-filled cuvettes and reagents, developed by our research laboratory, ready to use

• Pipettes• Print Manager, a software which enables to connect

the analyzer to the PC • User's manual• Illustrated data sheets• 3 years warranty

Features of the analyzer

• System: 4 wavelenghts and 12 incubation cells designed to heat samples at a temperature of 37°C

• Measurement: based on the reading of absorbance

• Photometric range: 0.0-2.3 Abs; (0.0-4.0 Abs) ampl.

• Hardware and Software: designed and manufactured by CDR S.r.l.

• Maintenance: not necessary periodic calibration

Tests

Tests

Competitors matrix

WineLab NIR External Laboratory

TraditionalSystems

Simplicity -

Analyzer price

Analysis price

Analysis speed

Calibration Not required Required - Required

- -Very HighLow

MediumMedium Low

High High Very Low

Medium

LowMedium High Low

Very Important!!

ISVEA validation

● CdR – WineLab system is validated by ISVEA

● This system doesn't need calibration. It is already calibrated and well correlated to the official methods

ISVEA validation

Acido Acetico (acidità volatile) su vino

R2 = 0,9709

0

0,2

0,4

0,6

0,8

1

1,2

1,4

0 0,2 0,4 0,6 0,8 1 1,2 1,4

WineLab (g/l ac. Acetico)

OIV

(g

/l a

c. A

ce

tic

o)

R2=0,97

R2=0,97

Volatile acidity (acetic acid)

Sugars

Acido L-Malico su vino

R2 = 0,9837

0

0,2

0,4

0,6

0,8

1

1,2

1,4

1,6

1,8

2

0 0,5 1 1,5 2

WineLab (g/l ac. Malico)

HP

LC

(g

/l ac.

Malic

o)

Malic Acid

R2=0,98

Glucose and fructose in wine

Fructose in wine

R2 = 0,9755

0

1

2

3

4

5

6

7

0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0

WineLab (g/l fructose)

HP

LC

(g

/l f

ruc

tos

e)

Glucose in wine

R2 = 0,9704

0

0,5

1

1,5

2

2,5

3

3,5

0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5

WineLab (g/l glucose)

HP

LC

(g

/l g

luc

ose

) R2=0,97R2=0,97

Glucose + ATP + NAD+ ADP + gluconate-6-P + NADH HK + G6P-DH

HK + G6P-DH + PGIFructose + ATP + NAD+ ADP + gluconate-6-P + NADH

Fructose in wine Glucose in wine

● Determination of assimilable sugars! Pentose sugars are excluded

● You have a detection limit of 0.1 g/L! You can know with high precision, in the alcohol fermentation, when the sugars are finished, to avoid any risk of acetic acid.

● You can do up to 30 analysis/hour.

CdR-WineLab's advantages

Glucose and fructose in wine

Free sulfur dioxide

Step 1: taking and incubation of the sample

Free sulfur dioxide

Step 2: addition of the R2 and reading of the sample

Inorganic Nitrogen(ammonium)

Organic nitrogen (α-aminic)

Yeast Assimilable Nitrogen

OHO

O

OHO

N+

H

H

HH

NADH

OHO

NH2

OHO

Gl-DHNAD

+

keto-glutarate

Ammonium

Glutammate

NH2

R O

OH

O

O

ONH

SH

OH

CH3

O

Amino acid OPA NAC isoindolic derivative

NR

OOH

S

R

Yeast Assimilable Nitrogen

● You can check separately the amount of inorganic nitrogen and organic nitrogen.

● The test is not affected by proline and hidroxy-proline interference.

● You can do up to 30 tests/hour.

CdR-WineLab's advantages

User Advantages

Small and medium wineries

•carry out independently the most important analysis on wine•affordable analyzer price•lower test price compared to laboratories

Big wineries

•carry out analysis on a small samples quantity without using expensive and complicated automatic analysis systems

Enological consultants•check the most important parameters during wine production in the wineries•create a “portable laboratory”

Thanks to its simplicity and reliability, WineLab can be used by:

Users

The NEXT NEW TEST

● It is available by the beginning of june

● The polyphenol index is a reading of absorbance at 280nm. So you can do this without using an UV lamp and without quarz cuvettes

● You can use this test in the mixture of wines to control and keep the right polyphenols level

TOTAL POLYPHENOL INDEX

WineColor

• WineColor is a complementar analyzer to do Colour Intensity and Tonality reading the absorbance at 420, 520, and 620 nm

• You can do these analysis without dilution of the sample

• You can control and correct the colour of the wine

Summarizing....

● WineLab is a rapid simple and reliable system. This analyzer is able to control each step of the vinification from grapes to wine in bottle

● The winery is autonomous for the analysis and you can have a result in real time without waiting for the result from external laboratory

● WineLab gives an enological consultant the opportunity to work without opening a laboratory

CdR- WineLab

Thanks for your attention

Doc. Simone Bellassai

R & D Chemist

s.bellassai@cdr-mediared.it