Finding a Therapeutic For AtherosclerosisKYLE MURRAYWESTERN MICHIGAN UNIVERSITY

Outline What is Atherosclerosis?

Objective

OxPAPC

Methods

Collected Data and Discussion

Future

What is Atherosclerosis? Initial Event of Heart Disease

What are the risk factors?

What are we Doing Differently?

Atherosclerotic Lesions

PAPC and OxPAPC

Biotin Tagged Lipids

MethodsSynthesize

Biotin Tagged Lipids

Solid Phase Extraction

Phosphorus Assay

Oxidation of PAPC

Phosphorus Assay

Western Blotting

Monitoring Oxidation

Key Proteins GRP78

EP2

VEGFR2

Western Blotting

Gel Electrophoresis Transfer Imaging

Identifying Binding to GRP78

Ideas For the Future Identify Key Proteins

Identify Gene Expression

Find Blocking Agent

Sizing LDL particles

Acknowledgments Dr. James Springstead

Dr. Andrew Kline

Dr. Betsey Aller

Abbie Brackman

QUESTIONS?