Wayne State Presentation 10-26-2014 (2)
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Transcript of Wayne State Presentation 10-26-2014 (2)
![Page 1: Wayne State Presentation 10-26-2014 (2)](https://reader036.fdocuments.in/reader036/viewer/2022062902/58eed8ae1a28ab1c1b8b460f/html5/thumbnails/1.jpg)
Finding a Therapeutic For AtherosclerosisKYLE MURRAYWESTERN MICHIGAN UNIVERSITY
![Page 2: Wayne State Presentation 10-26-2014 (2)](https://reader036.fdocuments.in/reader036/viewer/2022062902/58eed8ae1a28ab1c1b8b460f/html5/thumbnails/2.jpg)
Outline What is Atherosclerosis?
Objective
OxPAPC
Methods
Collected Data and Discussion
Future
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What is Atherosclerosis? Initial Event of Heart Disease
What are the risk factors?
What are we Doing Differently?
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Atherosclerotic Lesions
![Page 5: Wayne State Presentation 10-26-2014 (2)](https://reader036.fdocuments.in/reader036/viewer/2022062902/58eed8ae1a28ab1c1b8b460f/html5/thumbnails/5.jpg)
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PAPC and OxPAPC
![Page 7: Wayne State Presentation 10-26-2014 (2)](https://reader036.fdocuments.in/reader036/viewer/2022062902/58eed8ae1a28ab1c1b8b460f/html5/thumbnails/7.jpg)
Biotin Tagged Lipids
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MethodsSynthesize
Biotin Tagged Lipids
Solid Phase Extraction
Phosphorus Assay
Oxidation of PAPC
Phosphorus Assay
Western Blotting
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Monitoring Oxidation
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Key Proteins GRP78
EP2
VEGFR2
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Western Blotting
Gel Electrophoresis Transfer Imaging
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Identifying Binding to GRP78
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Ideas For the Future Identify Key Proteins
Identify Gene Expression
Find Blocking Agent
Sizing LDL particles
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Acknowledgments Dr. James Springstead
Dr. Andrew Kline
Dr. Betsey Aller
Abbie Brackman
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QUESTIONS?