©2011 Waters Corporation

Waters OverviewWaters Overview

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Introduction to WatersIntroduction to Waters

Established in 1958

Publicly traded corporation (NYSE:WAT)

$1.84 billion in annual revenue

Headquartered in Milford, Massachusetts

Manufacturing and distribution centers worldwide

5,700 employees many of whom were once customers

– Average of >10 years of service (>16 yrs in the field)

> 2,400 sales and service within the field organization

– maintains direct link with end users

Greatest proportion of revenue derived from new products

320$M

795$M

1.84$B

1994 2000 2012

All facilities cGMP-compliant & ISO 9001-2000 certified20,000 instruments180,000 columns1,400,000 parts / year

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1963First liquidseparations SystemGPC-150

1973High-performance liquid chromatography M6000 HPLC Pump

1978Solid phase ExtractionSep-Pak®

Cartridges

1988PC-based Chromatography softwareMaxima Software

1996Quadrupole Time-of-flight MSQ-TofTM

Mass Spectrometer

2004First UPLC® System ACQUITY UPLC® System

2006High-Definition MSSYNAPT HDMSTM

System

2008/2009XEVOTM TQ MSXEVO QTof MS

Breakthrough TechnologyBreakthrough Technology

2010ACQUITY H-class

Patrol

2012UPC2

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Application FocusApplication Focus

Pharmaceutical

Biopharmaceuticals

Chemical/Industrial

Environmental

Clinical

Food Safety

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Waters Technologies

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Waters Technologies

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“Demand for UHPLC systems has continued to outpace demand for other HPLC techniques. UHPLC systems are fast becoming the standard tool for research and routine applications.”

– Strategic Directions International

The ACQUITY Difference

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UPLC: Bridging the Gap From UPLC: Bridging the Gap From Laboratory to Manufacturing FloorLaboratory to Manufacturing Floor

Discovery/DevelopmentLab

QC Lab Process Lab ManufacturingFloor

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La più ampia scelta di colonne per UPLCLa più ampia scelta di colonne per UPLC

BEH C18

BEH C8

BEH Phenyl

BEH Shield RP18

BEH HILIC

BEH Amide

CSH C18

CSH Fluoro-Phenyl

HSS T3

HSS C18

HSS C18 SB

Cinque substrati differenti

• 130Å, 200Å e 300Å BEH [Ethylene Bridged Hybrid], HSS [High Strength Silica] e CSH [Charged Surface Hybrid]

• Tutte disponibili per HPLC e UPLC

Ampio spettro fasi stazionarie

• 18 fasi stazionarie

• BEH 130Å C18, C8, Shield RP18, Phenyl, HILIC ed Amide, IEX

• BEH 300Å C18 e C4

• BEH 200Å SEC

• HSS-C18, HSS-T3, HSSC18-SB, HSS-PFP, HSS-CN

• CSH C18, Fluoro-Phenyl e Phenyl-Hexyl

Colonne per applicazioni speciali

• AAA, OST, PST, PrST e Glycan

Transferibilità tra HPLC ed UPLC

XBridge HPLC = ACQUITY BEH

HSS HPLC and= ACQUITY HSS

XSelect HPLC = ACQUITY CSH CSH Phenyl-Hexyl

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Evolution of Separation TechnologyEvolution of Separation Technology

Gas Chromatography Liquid Chromatography Convergence Chromatography

GC

Capillary GC

HPLC

UPLC

SFC

UPC2

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The Advent of The Advent of Convergence ChromatographyConvergence Chromatography

Convergence Chromatography

UltraPerformance Convergence Chromatography is the result of significant technological advancements in Supercritical Fluid Chromatography instrumentation and chemistry design while providing exceptional increases in available selectivity

SFC UltraPerformanceConvergence Chromatography

Data courtesy of Davy Guillarme, Jean-Luc Veuthey LCAP, University of Geneva, Switzerland

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ACQUITY UPC² SystemACQUITY UPC² System

Built upon proven UPLC Technology– Quantifiable increase in productivity

Exceptional increase in available selectivity– Solve routine and complex separation challenges

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Where is CC Applicable?Where is CC Applicable?

Pharmaceutical and Life Sciences– Metabolite ID, stability-indicating, impurity

profiling

– Purity assessment, final product analysis

– Chiral

– Orthogonal method screening (vs. RPLC)

Food and environment– Vitamins, essential oils, pesticides, lipids,

triglycerides, food additives

Chemical materials– Polymers, organometallics, dyes,

petroleum, surfactants, petrochemicals, biodiesel

Clinical research– Vitamin D metabolites

– Steroids

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SQD2 XevoTQDXevoTQXevoTQ-S

Xevo G2 TofXevo G2 QTof

Synapt G2-SSynapt G2 HDMS

Soluzioni MS

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Sessioni Sessioni

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Grazie