John Crane Proprietary

- SMITHS CONFIDENTIAL -

The Oil Field Microbiome – How Can Metagenomics Help the Oil Industry?Isaac Newton Institute Open for Business event - 14th April 2014

Richard Johnson – Senior Technologist

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What are microbes doing in oil fields? - Souring

H2S

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What are microbes doing in oil fields? - Souring

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What are microbes doing in oil fields? - Corrosion

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What are microbes doing in oil fields? - Corrosion

From: Larsen, J., Rasmussen, K., Pedersen, H., Sørensen, K., Lundgaard, T. and Skovhus, T.L. 2010. “Consortia of MIC Bacteria and Archaea Causing Pitting Corrosion in Top Side Oil Production Facilities”, NACE Corrosion, 10252.

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What are microbes doing in oil fields? - Other

Filter plugging Reservoir plugging Reducing separation efficiency Reducing product quality

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An oil field cycle

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Monitoring and mitigation

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Analysis – A paradigm shift – Bug Bottles

SRB Test

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GAB/GAnB Test

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Analysis – A paradigm shift - qPCR

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Analysis – A paradigm shift - NGS

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How does this help?

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How can metagenomics help the oil industry?

Answer the questions...

What microorganisms are in there?How many are there (how big is the problem)?

What are they doing?How can we stop them?

Are there any trends/patterns?How interrelated are oil reservoirs/parts of a system?

John Crane Proprietary - SMITHS CONFIDENTIAL -

Thank you